Rmu_ssc0000114.1_g000055

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000114.1
OG0001158
Physical Location & Seq
Reverse (-)
286841 .. 292828
5988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000114.1_g000055.1.cds

Sequence Viewer

Length: 1446 bp
atgtgcaatgatagcgtcggagagggtttaaccctgaagcagatgaagcttaaaataccagcgaacgtaagtgttccggcagtgattgtgtttggagattcaatagttgatacgggcaacaataacaacaacttcataacatttgctcgctgcaatttcctcccttatggcatagacttccaaggtggacttgcaaccggacgattctcaaatggaaaggttcctgctgaccttgtagtggaagcatttggaattaaggagctgttgccaccctatatggatccatccctagagcctaatgatctccttactggtgttagctttgctgctggaggtgtaggttatgaccctttgacatcagaagtcgcggcagttgaatcactgtccgatcagctggaacaattcaaagggtacatagaaaagctgaaaggaatcgtcggagaaaagagaacaaacttcatcatatcaaatagtttgatttttgtggtagcaggaagcaacgacatttcgagtacctattttgttagtggtatcaggaaattgcatttcgatgttccttcttacaccgacttcttgctcaaccatgcttcgcaattcttcaaggaattatacggattgggtgcacgaagaataggcatttttggtgtaccagctattgggtgtgtgccatcccaaagaacaattggaggaggcatcggaagacgttgtgccgagaatcaaaaccaagcagcggagctcttcaactccaaactatctgcagagatgaatcaccttagacacacactccccaactcaaagttcgtttacatagacgtctacaacccattacttgatatcatcacaaactcaaataaatacgggtttgaagttgcagacaaagggtgttgtggtactggtttggtggaagtaacaaaactgtgcaaccagtttcagcgaacctgtttcgctgctacgtgtggcttggtaatttggatttggaatggtggtggctcttctctttcttctcccctacttggcgatgacgacgacgattggcttcgacggtgtcccatgatgttaggtttggatcgggttcgtccttttggtggatcaaagcctctgatagaggttatcttggctgtgaaggcgctagacttctcgccttcaatctcatcggtgttaggcggagctgggccttttgctttgggcaatgtcggatggggacttgatgatggcaggactgctcaagtctatttgggttcaaattcgattgggtgcactttggcttctgtctctaagatcatcggcttggatttggtgagttgtcccgatggttggatcgatgggaccggcaaccatatgactggttttcagccttggtctgggggcgacgacgattcttggttgggatctaattttcttaatgtcaaggcaggcagcggtggtgcagtggcagtgctgagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

481

Amino Acids

51.25

Weight (kDa)

5.08

Isoelectric Point (pI)

24.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 816
AccB7I CCANNNNNTGG 1 cut(s) 656
AccI GTMKAC 1 cut(s) 816
AccII CGCG 1 cut(s) 368
AciI CCGC 4 cut(s) 368, 731, 1164, 1419
AclWI GGATC 6 cut(s) 275, 288, 1074, 1096, 1325, 1396
AcsI RAATTY 1 cut(s) 1243
AcuI CTGAAG 1 cut(s) 56
AcyI GRCGYC 1 cut(s) 813
AfaI GTAC 4 cut(s) 413, 514, 648, 892
AfiI CCNNNNNNNGG 7 cut(s) 238, 656, 730, 1013, 1085, 1314, 1361
AflIII ACRYGT 1 cut(s) 953
AgsI TTSAA 8 cut(s) 102, 377, 406, 601, 742, 866, 1146, 1242
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AluBI AGCT 9 cut(s) 49, 262, 321, 394, 424, 653, 736, 1169, 1443
AluI AGCT 9 cut(s) 49, 262, 321, 394, 424, 653, 736, 1169, 1443
Alw21I GWGCWC 3 cut(s) 625, 738, 1259
Alw26I GTCTC 1 cut(s) 1276
Alw44I GTGCAC 2 cut(s) 621, 1255
AlwI GGATC 6 cut(s) 275, 288, 1074, 1096, 1325, 1396
AoxI GGCC 1 cut(s) 1172
ApaLI GTGCAC 2 cut(s) 621, 1255
ApeKI GCWGC 5 cut(s) 150, 326, 728, 947, 1416
ApoI RAATTY 1 cut(s) 1243
AspLEI GCGC 1 cut(s) 1129
AspS9I GGNCC 2 cut(s) 1172, 1326
AsuHPI GGTGA 2 cut(s) 761, 1309
AvaII GGWCC 1 cut(s) 1326
BaeGI GKGCMC 2 cut(s) 625, 1259
BamHI GGATCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 706
Bbv12I GWGCWC 3 cut(s) 625, 738, 1259
BbvI GCAGC 5 cut(s) 137, 313, 740, 934, 1428
BccI CCATC 6 cut(s) 292, 676, 1191, 1205, 1304, 1316
BcoDI GTCTC 1 cut(s) 1276
BfaI CTAG 3 cut(s) 290, 1130, 1444
BfmI CTRYAG 1 cut(s) 756
BfoI RGCGCY 1 cut(s) 1130
BisI GCNGC 6 cut(s) 151, 327, 369, 729, 948, 1417
BlpI GCTNAGC 1 cut(s) 1439
BlsI GCNGC 6 cut(s) 152, 328, 370, 730, 949, 1418
Bme18I GGWCC 1 cut(s) 1326
BmgT120I GGNCC 2 cut(s) 1172, 1326
BmiI GGNNCC 3 cut(s) 222, 282, 1327
BmsI GCATC 1 cut(s) 702
BpiI GAAGAC 1 cut(s) 706
BpmI CTGGAG 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 1439
BpuEI CTTGAG 1 cut(s) 1209
Bsa29I ATCGAT 1 cut(s) 1320
BsaAI YACGTR 1 cut(s) 954
BsaHI GRCGYC 1 cut(s) 813
BsaJI CCNNGG 2 cut(s) 181, 1355
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bsc4I CCNNNNNNNGG 7 cut(s) 238, 656, 730, 1013, 1085, 1314, 1361
Bse118I RCCGGY 1 cut(s) 1328
Bse1I ACTGG 4 cut(s) 316, 898, 925, 1348
Bse3DI GCAATG 2 cut(s) 13, 1195
BseCI ATCGAT 1 cut(s) 1320
BseDI CCNNGG 2 cut(s) 181, 1355
BseGI GGATG 3 cut(s) 284, 668, 1202
BseLI CCNNNNNNNGG 7 cut(s) 238, 656, 730, 1013, 1085, 1314, 1361
BseMI GCAATG 2 cut(s) 13, 1195
BseMII CTCAG 1 cut(s) 1430
BseNI ACTGG 4 cut(s) 316, 898, 925, 1348
BseRI GAGGAG 1 cut(s) 702
BseSI GKGCMC 2 cut(s) 625, 1259
BseXI GCAGC 5 cut(s) 137, 313, 740, 934, 1428
BseYI CCCAGC 1 cut(s) 1169
BsgI GTGCAG 1 cut(s) 1446
Bsh1236I CGCG 1 cut(s) 368
BshFI GGCC 1 cut(s) 1174
BshVI ATCGAT 1 cut(s) 1320
BsiHKAI GWGCWC 3 cut(s) 625, 738, 1259
BsiSI CCGG 3 cut(s) 77, 198, 1329
BslFI GGGAC 4 cut(s) 1032, 1215, 1290, 1339
BslI CCNNNNNNNGG 7 cut(s) 238, 656, 730, 1013, 1085, 1314, 1361
BsmAI GTCTC 1 cut(s) 1276
BsmFI GGGAC 4 cut(s) 1032, 1215, 1290, 1339
BsnI GGCC 1 cut(s) 1174
Bsp1286I GDGCHC 3 cut(s) 625, 738, 1259
Bsp143I GATC 8 cut(s) 280, 301, 388, 1066, 1088, 1278, 1317, 1388
Bsp1720I GCTNAGC 1 cut(s) 1439
BspACI CCGC 4 cut(s) 368, 731, 1164, 1419
BspANI GGCC 1 cut(s) 1174
BspCNI CTCAG 1 cut(s) 1431
BspDI ATCGAT 1 cut(s) 1320
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 3 cut(s) 222, 282, 1327
BspMAI CTGCAG 1 cut(s) 760
BspPI GGATC 6 cut(s) 275, 288, 1074, 1096, 1325, 1396
BspQI GCTCTTC 2 cut(s) 743, 997
BsrDI GCAATG 2 cut(s) 13, 1195
BsrFI RCCGGY 1 cut(s) 1328
BsrI ACTGG 4 cut(s) 316, 898, 925, 1348
BssAI RCCGGY 1 cut(s) 1328
BssECI CCNNGG 2 cut(s) 181, 1355
BssMI GATC 8 cut(s) 280, 301, 388, 1066, 1088, 1278, 1317, 1388
BssNI GRCGYC 1 cut(s) 813
BssT1I CCWWGG 2 cut(s) 181, 1355
Bst4CI ACNGT 3 cut(s) 384, 918, 1044
Bst6I CTCTTC 2 cut(s) 743, 997
BstACI GRCGYC 1 cut(s) 813
BstBAI YACGTR 1 cut(s) 954
BstC8I GCNNGC 2 cut(s) 148, 1414
BstDEI CTNAG 3 cut(s) 773, 1275, 1439
BstF5I GGATG 3 cut(s) 284, 668, 1202
BstFNI CGCG 1 cut(s) 368
BstH2I RGCGCY 1 cut(s) 1130
BstHHI GCGC 1 cut(s) 1129
BstKTI GATC 8 cut(s) 283, 304, 391, 1069, 1091, 1281, 1320, 1391
BstMAI GTCTC 1 cut(s) 1276
BstMBI GATC 8 cut(s) 280, 301, 388, 1066, 1088, 1278, 1317, 1388
BstMWI GCNNNNNNNGC 3 cut(s) 12, 46, 1124
BstSFI CTRYAG 1 cut(s) 756
BstSLI GKGCMC 2 cut(s) 625, 1259
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 5 cut(s) 137, 313, 740, 934, 1428
BstV2I GAAGAC 1 cut(s) 706
BstX2I RGATCY 2 cut(s) 280, 1388
BstXI CCANNNNNNTGG 1 cut(s) 1343
BstYI RGATCY 2 cut(s) 280, 1388
Bsu15I ATCGAT 1 cut(s) 1320
BsuRI GGCC 1 cut(s) 1174
BsuTUI ATCGAT 1 cut(s) 1320
BtgZI GCGATG 1 cut(s) 1032
BtsCI GGATG 3 cut(s) 284, 668, 1202
BtsI GCAGTG 3 cut(s) 87, 1434, 1440
BtsIMutI CAGTG 4 cut(s) 87, 380, 1434, 1440
Cac8I GCNNGC 2 cut(s) 148, 1414
CfoI GCGC 1 cut(s) 1129
Cfr10I RCCGGY 1 cut(s) 1328
Cfr13I GGNCC 2 cut(s) 1172, 1326
ClaI ATCGAT 1 cut(s) 1320
CseI GACGC 1 cut(s) 4
Csp6I GTAC 4 cut(s) 412, 513, 647, 891
CviAII CATG 2 cut(s) 584, 1051
CviQI GTAC 4 cut(s) 412, 513, 647, 891
DdeI CTNAG 3 cut(s) 773, 1275, 1439
DpnI GATC 8 cut(s) 282, 303, 390, 1068, 1090, 1280, 1319, 1390
DpnII GATC 8 cut(s) 280, 301, 388, 1066, 1088, 1278, 1317, 1388
Eam1104I CTCTTC 2 cut(s) 743, 997
EarI CTCTTC 2 cut(s) 743, 997
EciI GGCGGA 1 cut(s) 1179
Ecl136II GAGCTC 1 cut(s) 736
Eco130I CCWWGG 2 cut(s) 181, 1355
Eco24I GRGCYC 1 cut(s) 738
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 1326
Eco53kI GAGCTC 1 cut(s) 736
Eco57I CTGAAG 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 736
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 2 cut(s) 181, 1355
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 2 cut(s) 181, 1355
FaeI CATG 2 cut(s) 587, 1054
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 4 cut(s) 1032, 1215, 1290, 1339
FatI CATG 2 cut(s) 583, 1050
FauNDI CATATG 1 cut(s) 1338
FblI GTMKAC 1 cut(s) 816
Fnu4HI GCNGC 6 cut(s) 151, 327, 369, 729, 948, 1417
FokI GGATG 3 cut(s) 271, 655, 1209
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 6 cut(s) 151, 327, 369, 729, 948, 1417
FspBI CTAG 3 cut(s) 290, 1130, 1444
GlaI GCGC 1 cut(s) 1128
GluI GCNGC 6 cut(s) 151, 327, 369, 729, 948, 1417
GsaI CCCAGC 1 cut(s) 1173
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 1130
HaeIII GGCC 1 cut(s) 1174
HapII CCGG 3 cut(s) 77, 198, 1329
HgaI GACGC 1 cut(s) 4
HhaI GCGC 1 cut(s) 1129
Hin1I GRCGYC 1 cut(s) 813
Hin1II CATG 2 cut(s) 587, 1054
Hin6I GCGC 1 cut(s) 1127
HinP1I GCGC 1 cut(s) 1127
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 7 cut(s) 98, 204, 377, 432, 715, 766, 1376
HpaII CCGG 3 cut(s) 77, 198, 1329
HphI GGTGA 2 cut(s) 761, 1309
Hpy166II GTNNAC 6 cut(s) 188, 623, 647, 805, 817, 1257
Hpy188I TCNGA 7 cut(s) 20, 361, 388, 440, 698, 1101, 1196
Hpy188III TCNNGA 2 cut(s) 535, 1307
Hpy8I GTNNAC 6 cut(s) 188, 623, 647, 805, 817, 1257
Hpy99I CGWCG 7 cut(s) 20, 440, 1028, 1031, 1044, 1373, 1376
HpyAV CCTTC 3 cut(s) 567, 1117, 1152
HpyCH4III ACNGT 3 cut(s) 384, 918, 1044
HpyCH4IV ACGT 4 cut(s) 66, 703, 813, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 46, 1124
HpyF3I CTNAG 3 cut(s) 773, 1275, 1439
HpySE526I ACGT 4 cut(s) 66, 703, 813, 953
Hsp92I GRCGYC 1 cut(s) 813
Hsp92II CATG 2 cut(s) 587, 1054
HspAI GCGC 1 cut(s) 1127
Kzo9I GATC 8 cut(s) 280, 301, 388, 1066, 1088, 1278, 1317, 1388
LguI GCTCTTC 2 cut(s) 743, 997
LmnI GCTCC 3 cut(s) 259, 733, 1166
Lsp1109I GCAGC 5 cut(s) 137, 313, 740, 934, 1428
LweI GCATC 1 cut(s) 702
MaeI CTAG 3 cut(s) 290, 1130, 1444
MaeII ACGT 4 cut(s) 66, 703, 813, 953
MaeIII GTNAC 1 cut(s) 907
MalI GATC 8 cut(s) 282, 303, 390, 1068, 1090, 1280, 1319, 1390
MboI GATC 8 cut(s) 280, 301, 388, 1066, 1088, 1278, 1317, 1388
MboII GAAGA 6 cut(s) 589, 639, 711, 730, 984, 993
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 2 cut(s) 280, 1388
MhlI GDGCHC 3 cut(s) 625, 738, 1259
MmeI TCCRAC 3 cut(s) 418, 1174, 1295
MnlI CCTC 7 cut(s) 16, 170, 326, 680, 683, 1099, 1107
MseI TTAA 4 cut(s) 29, 51, 255, 1401
MslI CAYNNNNRTG 1 cut(s) 549
MspA1I CMGCKG 3 cut(s) 394, 731, 1419
MspI CCGG 3 cut(s) 77, 198, 1329
MunI CAATTG 1 cut(s) 681
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 3 cut(s) 12, 46, 1124
NdeI CATATG 1 cut(s) 1338
NdeII GATC 8 cut(s) 280, 301, 388, 1066, 1088, 1278, 1317, 1388
NlaIII CATG 2 cut(s) 587, 1054
NlaIV GGNNCC 3 cut(s) 222, 282, 1327
NmeAIII GCCGAG 1 cut(s) 736
PciSI GCTCTTC 2 cut(s) 743, 997
PfeI GAWTC 7 cut(s) 98, 204, 377, 432, 715, 766, 1376
PflFI GACNNNGTC 1 cut(s) 1044
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 6 cut(s) 152, 328, 370, 730, 949, 1418
Ppu21I YACGTR 1 cut(s) 954
Psp124BI GAGCTC 1 cut(s) 738
PspFI CCCAGC 1 cut(s) 1169
PspN4I GGNNCC 3 cut(s) 222, 282, 1327
PspPI GGNCC 2 cut(s) 1172, 1326
PstI CTGCAG 1 cut(s) 760
PsuI RGATCY 2 cut(s) 280, 1388
PsyI GACNNNGTC 1 cut(s) 1044
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 4 cut(s) 413, 514, 648, 892
RsaNI GTAC 4 cut(s) 412, 513, 647, 891
RseI CAYNNNNRTG 1 cut(s) 549
SacI GAGCTC 1 cut(s) 738
SapI GCTCTTC 2 cut(s) 743, 997
SaqAI TTAA 4 cut(s) 29, 51, 255, 1401
SatI GCNGC 6 cut(s) 151, 327, 369, 729, 948, 1417
Sau3AI GATC 8 cut(s) 280, 301, 388, 1066, 1088, 1278, 1317, 1388
Sau96I GGNCC 2 cut(s) 1172, 1326
SduI GDGCHC 3 cut(s) 625, 738, 1259
SfaNI GCATC 1 cut(s) 702
SfcI CTRYAG 1 cut(s) 756
SinI GGWCC 1 cut(s) 1326
SmiMI CAYNNNNRTG 1 cut(s) 549
SmlI CTYRAG 1 cut(s) 1224
SmoI CTYRAG 1 cut(s) 1224
SsiI CCGC 4 cut(s) 368, 731, 1164, 1419
SspMI CTAG 3 cut(s) 290, 1130, 1444
SstI GAGCTC 1 cut(s) 738
StyI CCWWGG 2 cut(s) 181, 1355
TaaI ACNGT 3 cut(s) 384, 918, 1044
TaiI ACGT 4 cut(s) 69, 706, 816, 956
TaqI TCGA 5 cut(s) 509, 549, 1039, 1247, 1320
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 7 cut(s) 98, 204, 377, 432, 715, 766, 1376
Tru1I TTAA 4 cut(s) 29, 51, 255, 1401
Tru9I TTAA 4 cut(s) 29, 51, 255, 1401
TscAI CASTG 4 cut(s) 87, 387, 1434, 1440
TseI GCWGC 5 cut(s) 150, 326, 728, 947, 1416
TspDTI ATGAA 4 cut(s) 59, 124, 448, 779
TspGWI ACGGA 1 cut(s) 627
TspRI CASTG 4 cut(s) 87, 387, 1434, 1440
Tth111I GACNNNGTC 1 cut(s) 1044
Van91I CCANNNNNTGG 1 cut(s) 656
VneI GTGCAC 2 cut(s) 621, 1255
VpaK11BI GGWCC 1 cut(s) 1326
XapI RAATTY 1 cut(s) 1243
XcmI CCANNNNNNNNNTGG 1 cut(s) 680
XmiI GTMKAC 1 cut(s) 816
XspI CTAG 3 cut(s) 290, 1130, 1444
ZraI GACGTC 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.