Rmu_ssc0000114.1_g000059

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000114.1
OG0001158
Physical Location & Seq
Reverse (-)
308396 .. 310260
1865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000114.1_g000059.1.cds

Sequence Viewer

Length: 1173 bp
atgcttcctctttggaccggaggaaattccaaaagaatgggattcctccttatagaaaccttctcttcttcttcttcctctggttccataatgtcgtcttcgaagcagctcttgatatttgtttgtagtcttgctgtggttttagggcagtaccccagaatcacggagggtcgggtgaatataccaccaggaaagtcagtgccggcggttatagtgttcggtgattcgatcatggacaccggcaacaataacaatcttaactcggctgttaaatgcaatttccctccatatggtgaaaatttcccaggaaaagtttcaaccggtagatttggcaatggaaaagtcccctcagacttcatagctcaagaactgggaatcaaagagtttgtgccggcatatgcggatactagtctaaaacctgaagatctcagcactggagtctgcttcgcttcaggtggcaccggatatgaccctcagacccccgaattagtaggagttatatcgctatcggagcagttgaacatgttcaaggagtatatagggaagctgaaagccaattttggcgagcagagaacaagcttcatcctcgccaacagtctctttctggtggtcgccggtagcgatgacattgccaacacctatttcactattggcatcagaaaggcagaatatgacgtccctgcttacactgaccttatggtcaactctgccactagtttccttcaggatttgtatgcacttggggctagaaggttcggtgttttcagtgcaccaccgatagggtgtgtaccttcacaaagaactctaggtggaggaatacaaagacagtgtgctgaagagtacaacgacgcagcgaagctattcaactctaagctgtcgtccaacatgcagtccttgaatacaaagctgcccgacagtagaatggtttatatcgatgtttacacccctctacttgatgtcattcttcaccctgccaaatacgggtttaaaatggtggagaaaggttgctgtggcacaggggcactagaggtagcgatattatgcaacaaattgtctccaacgtgtgaaaatccggcggatcatgtattttgggatagttatcaccctactgaacaagcatacagagtactcattcctccagttctccaaaaatacgtaaaccaattcttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

42.43

Weight (kDa)

6.01

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 698
AatII GACGTC 1 cut(s) 678
AccB1I GGYRCC 1 cut(s) 458
AciI CCGC 3 cut(s) 206, 401, 1076
AclWI GGATC 1 cut(s) 1086
AcsI RAATTY 2 cut(s) 25, 298
AcuI CTGAAG 4 cut(s) 435, 441, 707, 855
AcyI GRCGYC 1 cut(s) 675
AfaI GTAC 4 cut(s) 152, 789, 842, 1128
AfiI CCNNNNNNNGG 3 cut(s) 290, 779, 981
AflIII ACRYGT 2 cut(s) 522, 1061
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 5 cut(s) 318, 520, 529, 865, 898
AhlI ACTAGT 2 cut(s) 407, 713
AjnI CCWGG 2 cut(s) 187, 304
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AluBI AGCT 7 cut(s) 109, 362, 547, 579, 859, 874, 907
AluI AGCT 7 cut(s) 109, 362, 547, 579, 859, 874, 907
Alw21I GWGCWC 1 cut(s) 772
Alw26I GTCTC 2 cut(s) 602, 1059
Alw44I GTGCAC 1 cut(s) 768
AlwI GGATC 1 cut(s) 1086
ApaLI GTGCAC 1 cut(s) 768
ApeKI GCWGC 3 cut(s) 106, 851, 907
ApoI RAATTY 2 cut(s) 25, 298
AsiGI ACCGGT 1 cut(s) 320
Asp700I GAANNNNTTC 3 cut(s) 313, 524, 860
AspS9I GGNCC 1 cut(s) 15
AsuHPI GGTGA 5 cut(s) 187, 233, 305, 959, 1094
AsuII TTCGAA 1 cut(s) 101
AvaII GGWCC 1 cut(s) 15
BaeGI GKGCMC 2 cut(s) 772, 1024
BanI GGYRCC 1 cut(s) 458
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 772
BbvI GCAGC 3 cut(s) 118, 863, 894
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 2 cut(s) 189, 306
BciVI GTATCC 1 cut(s) 397
BcoDI GTCTC 2 cut(s) 602, 1059
BcuI ACTAGT 2 cut(s) 407, 713
BfaI CTAG 5 cut(s) 408, 714, 747, 806, 1025
BfuI GTATCC 1 cut(s) 397
BglII AGATCT 1 cut(s) 424
BisI GCNGC 3 cut(s) 107, 852, 908
BlsI GCNGC 3 cut(s) 108, 853, 909
BmcAI AGTACT 1 cut(s) 1128
Bme1390I CCNGG 2 cut(s) 189, 306
Bme18I GGWCC 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 85, 460
BmrFI CCNGG 2 cut(s) 189, 306
BmrI ACTGGG 1 cut(s) 380
BmsI GCATC 1 cut(s) 663
BmuI ACTGGG 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 2 cut(s) 456, 1122
Bpu14I TTCGAA 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 348
Bsa29I ATCGAT 1 cut(s) 933
BsaAI YACGTR 1 cut(s) 1156
BsaBI GATNNNNATC 1 cut(s) 1098
BsaHI GRCGYC 1 cut(s) 675
BsaJI CCNNGG 1 cut(s) 304
BsaWI WCCGGW 3 cut(s) 17, 320, 461
Bsc4I CCNNNNNNNGG 3 cut(s) 290, 779, 981
Bse118I RCCGGY 5 cut(s) 202, 239, 320, 391, 614
Bse1I ACTGG 3 cut(s) 375, 439, 1139
Bse3DI GCAATG 2 cut(s) 340, 627
Bse8I GATNNNNATC 1 cut(s) 1098
BseBI CCWGG 2 cut(s) 189, 306
BseCI ATCGAT 1 cut(s) 933
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 582
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 3 cut(s) 290, 779, 981
BseMI GCAATG 2 cut(s) 340, 627
BseMII CTCAG 3 cut(s) 363, 442, 488
BseNI ACTGG 3 cut(s) 375, 439, 1139
BseSI GKGCMC 2 cut(s) 772, 1024
BseXI GCAGC 3 cut(s) 118, 863, 894
BshNI GGYRCC 1 cut(s) 458
BshTI ACCGGT 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 933
BsiHKAI GWGCWC 1 cut(s) 772
BsiSI CCGG 8 cut(s) 18, 203, 240, 321, 392, 462, 615, 1073
BslFI GGGAC 2 cut(s) 329, 662
BslI CCNNNNNNNGG 3 cut(s) 290, 779, 981
BsmAI GTCTC 2 cut(s) 602, 1059
BsmFI GGGAC 2 cut(s) 329, 662
Bsp119I TTCGAA 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 772, 1024
Bsp143I GATC 3 cut(s) 228, 424, 1078
BspACI CCGC 3 cut(s) 206, 401, 1076
BspCNI CTCAG 3 cut(s) 362, 441, 487
BspDI ATCGAT 1 cut(s) 933
BspLI GGNNCC 2 cut(s) 85, 460
BspPI GGATC 1 cut(s) 1086
BspT104I TTCGAA 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 340, 627
BsrFI RCCGGY 5 cut(s) 202, 239, 320, 391, 614
BsrI ACTGG 3 cut(s) 375, 439, 1139
BssAI RCCGGY 5 cut(s) 202, 239, 320, 391, 614
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 3 cut(s) 228, 424, 1078
BssNI GRCGYC 1 cut(s) 675
Bst2UI CCWGG 2 cut(s) 189, 306
Bst4CI ACNGT 3 cut(s) 596, 828, 917
Bst6I CTCTTC 2 cut(s) 70, 831
BstACI GRCGYC 1 cut(s) 675
BstBAI YACGTR 1 cut(s) 1156
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 3 cut(s) 204, 393, 566
BstDEI CTNAG 4 cut(s) 349, 428, 474, 870
BstF5I GGATG 1 cut(s) 582
BstKTI GATC 3 cut(s) 231, 427, 1081
BstMAI GTCTC 2 cut(s) 602, 1059
BstMBI GATC 3 cut(s) 228, 424, 1078
BstMWI GCNNNNNNNGC 2 cut(s) 511, 743
BstNI CCWGG 2 cut(s) 189, 306
BstNSI RCATGY 2 cut(s) 526, 889
BstSCI CCNGG 2 cut(s) 187, 304
BstSLI GKGCMC 2 cut(s) 772, 1024
BstSNI TACGTA 1 cut(s) 1156
BstV1I GCAGC 3 cut(s) 118, 863, 894
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 424
BstXI CCANNNNNNTGG 1 cut(s) 37
BstYI RGATCY 1 cut(s) 424
Bsu15I ATCGAT 1 cut(s) 933
BsuI GTATCC 1 cut(s) 397
BsuTUI ATCGAT 1 cut(s) 933
BtgZI GCGATG 1 cut(s) 636
BtsCI GGATG 1 cut(s) 582
BtsIMutI CAGTG 5 cut(s) 204, 432, 687, 772, 833
Cac8I GCNNGC 3 cut(s) 204, 393, 566
Cfr10I RCCGGY 5 cut(s) 202, 239, 320, 391, 614
Cfr13I GGNCC 1 cut(s) 15
ClaI ATCGAT 1 cut(s) 933
CseI GACGC 1 cut(s) 857
Csp6I GTAC 4 cut(s) 151, 788, 841, 1127
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 4 cut(s) 232, 523, 886, 1082
CviQI GTAC 4 cut(s) 151, 788, 841, 1127
DdeI CTNAG 4 cut(s) 349, 428, 474, 870
DpnI GATC 3 cut(s) 230, 426, 1080
DpnII GATC 3 cut(s) 228, 424, 1078
DraI TTTAAA 1 cut(s) 988
DrdI GACNNNNNNGTC 1 cut(s) 698
DseDI GACNNNNNNGTC 1 cut(s) 698
Eam1104I CTCTTC 2 cut(s) 70, 831
EarI CTCTTC 2 cut(s) 70, 831
EciI GGCGGA 1 cut(s) 1091
Eco105I TACGTA 1 cut(s) 1156
Eco47I GGWCC 1 cut(s) 15
Eco57I CTGAAG 4 cut(s) 435, 441, 707, 855
EcoRII CCWGG 2 cut(s) 187, 304
FaeI CATG 4 cut(s) 235, 526, 889, 1085
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 2 cut(s) 329, 662
FatI CATG 4 cut(s) 231, 522, 885, 1081
FauNDI CATATG 2 cut(s) 289, 397
Fnu4HI GCNGC 3 cut(s) 107, 852, 908
FokI GGATG 1 cut(s) 569
Fsp4HI GCNGC 3 cut(s) 107, 852, 908
FspBI CTAG 5 cut(s) 408, 714, 747, 806, 1025
GluI GCNGC 3 cut(s) 107, 852, 908
GsuI CTGGAG 2 cut(s) 456, 1122
HapII CCGG 8 cut(s) 18, 203, 240, 321, 392, 462, 615, 1073
HgaI GACGC 1 cut(s) 857
Hin1I GRCGYC 1 cut(s) 675
Hin1II CATG 4 cut(s) 235, 526, 889, 1085
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HindIII AAGCTT 1 cut(s) 577
HinfI GANTC 5 cut(s) 42, 159, 224, 375, 438
HpaII CCGG 8 cut(s) 18, 203, 240, 321, 392, 462, 615, 1073
HphI GGTGA 5 cut(s) 187, 233, 305, 959, 1094
Hpy166II GTNNAC 5 cut(s) 703, 770, 788, 940, 1159
Hpy188I TCNGA 4 cut(s) 352, 477, 511, 659
Hpy188III TCNNGA 3 cut(s) 112, 365, 725
Hpy8I GTNNAC 5 cut(s) 703, 770, 788, 940, 1159
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 4 cut(s) 70, 731, 744, 801
HpyCH4III ACNGT 3 cut(s) 596, 828, 917
HpyCH4IV ACGT 3 cut(s) 675, 1061, 1155
HpyCH4V TGCA 5 cut(s) 276, 737, 770, 889, 1044
HpyF10VI GCNNNNNNNGC 2 cut(s) 511, 743
HpyF3I CTNAG 4 cut(s) 349, 428, 474, 870
HpySE526I ACGT 3 cut(s) 675, 1061, 1155
Hsp92I GRCGYC 1 cut(s) 675
Hsp92II CATG 4 cut(s) 235, 526, 889, 1085
KroI GCCGGC 2 cut(s) 202, 391
KroNI GCCGGC 2 cut(s) 204, 393
Kzo9I GATC 3 cut(s) 228, 424, 1078
LmnI GCTCC 1 cut(s) 511
Lsp1109I GCAGC 3 cut(s) 118, 863, 894
LweI GCATC 1 cut(s) 663
MaeI CTAG 5 cut(s) 408, 714, 747, 806, 1025
MaeII ACGT 3 cut(s) 675, 1061, 1155
MalI GATC 3 cut(s) 230, 426, 1080
MboI GATC 3 cut(s) 228, 424, 1078
MboII GAAGA 8 cut(s) 57, 60, 63, 66, 90, 434, 848, 956
MflI RGATCY 1 cut(s) 424
MhlI GDGCHC 2 cut(s) 772, 1024
MluCI AATT 7 cut(s) 25, 277, 298, 485, 556, 1049, 1163
MlyI GAGTC 1 cut(s) 447
MmeI TCCRAC 2 cut(s) 906, 1082
MroNI GCCGGC 2 cut(s) 202, 391
MroXI GAANNNNTTC 3 cut(s) 313, 524, 860
MseI TTAA 3 cut(s) 258, 270, 987
MspI CCGG 8 cut(s) 18, 203, 240, 321, 392, 462, 615, 1073
MspR9I CCNGG 2 cut(s) 189, 306
MvaI CCWGG 2 cut(s) 189, 306
MwoI GCNNNNNNNGC 2 cut(s) 511, 743
NaeI GCCGGC 2 cut(s) 204, 393
NdeI CATATG 2 cut(s) 289, 397
NdeII GATC 3 cut(s) 228, 424, 1078
NgoMIV GCCGGC 2 cut(s) 202, 391
NlaIII CATG 4 cut(s) 235, 526, 889, 1085
NlaIV GGNNCC 2 cut(s) 85, 460
NmeAIII GCCGAG 1 cut(s) 242
NspI RCATGY 2 cut(s) 526, 889
NspV TTCGAA 1 cut(s) 101
PciI ACATGT 1 cut(s) 522
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PdiI GCCGGC 2 cut(s) 204, 393
PdmI GAANNNNTTC 3 cut(s) 313, 524, 860
PfeI GAWTC 4 cut(s) 42, 159, 224, 375
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 3 cut(s) 108, 853, 909
PleI GAGTC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 446
Ppu21I YACGTR 1 cut(s) 1156
PscI ACATGT 1 cut(s) 522
Psp6I CCWGG 2 cut(s) 187, 304
PspGI CCWGG 2 cut(s) 187, 304
PspN4I GGNNCC 2 cut(s) 85, 460
PspPI GGNCC 1 cut(s) 15
PsuI RGATCY 1 cut(s) 424
RsaI GTAC 4 cut(s) 152, 789, 842, 1128
RsaNI GTAC 4 cut(s) 151, 788, 841, 1127
SaqAI TTAA 3 cut(s) 258, 270, 987
SatI GCNGC 3 cut(s) 107, 852, 908
Sau3AI GATC 3 cut(s) 228, 424, 1078
Sau96I GGNCC 1 cut(s) 15
ScaI AGTACT 1 cut(s) 1128
SchI GAGTC 1 cut(s) 447
ScrFI CCNGG 2 cut(s) 189, 306
SduI GDGCHC 2 cut(s) 772, 1024
SfaNI GCATC 1 cut(s) 663
SfuI TTCGAA 1 cut(s) 101
SinI GGWCC 1 cut(s) 15
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
SnaBI TACGTA 1 cut(s) 1156
SpeI ACTAGT 2 cut(s) 407, 713
Sse9I AATT 7 cut(s) 25, 277, 298, 485, 556, 1049, 1163
SsiI CCGC 3 cut(s) 206, 401, 1076
SspMI CTAG 5 cut(s) 408, 714, 747, 806, 1025
StyD4I CCNGG 2 cut(s) 187, 304
TaaI ACNGT 3 cut(s) 596, 828, 917
TaiI ACGT 3 cut(s) 678, 1064, 1158
TaqI TCGA 3 cut(s) 101, 227, 933
TasI AATT 7 cut(s) 25, 277, 298, 485, 556, 1049, 1163
TatI WGTACW 2 cut(s) 840, 1126
TfiI GAWTC 4 cut(s) 42, 159, 224, 375
Tru1I TTAA 3 cut(s) 258, 270, 987
Tru9I TTAA 3 cut(s) 258, 270, 987
TscAI CASTG 5 cut(s) 204, 439, 694, 772, 833
TseI GCWGC 3 cut(s) 106, 851, 907
TspDTI ATGAA 2 cut(s) 346, 571
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 5 cut(s) 204, 439, 694, 772, 833
VneI GTGCAC 1 cut(s) 768
VpaK11BI GGWCC 1 cut(s) 15
XapI RAATTY 2 cut(s) 25, 298
XceI RCATGY 2 cut(s) 526, 889
XmnI GAANNNNTTC 3 cut(s) 313, 524, 860
XspI CTAG 5 cut(s) 408, 714, 747, 806, 1025
ZraI GACGTC 1 cut(s) 676
ZrmI AGTACT 1 cut(s) 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.