pycom08g00830

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001158
Physical Location & Seq
Reverse (-)
639187 .. 642646
3460 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00830.4

Sequence Viewer

Length: 1143 bp
ATGGTATCAGGATTAATTGTTATGGAGACTTCTTCCTCTTCTTCTTGTCCTTCTCGGTTGCTGTCGTCCCGGTTGATATTATGTGTTTGTGTCGTAGTTTTTGGTGTTTTCCAGTACCCTACAGCCACAGAGGGACTGGTGAAGCTGCCACCAAACCAGACTGTTCCGGCGGTGATCGTGTTCGGCGATTCGATCATGGACACGGGCAACAACAATGATCTCGAGTCACTTGTTAGGTGCAATTTCCCTCCTTATGGACTAGATTTCCAGCAGCACAAGCCTACTGGCAGGTTTGGCAATGGCAAAGTGCCCTCAGACCTCATAGCGTCAGAATTGGGAATCCGAGAGTATTTGCCGGCATATTTGGATCCAAATCTACGACCTCAAGATCTCCCCACCGGAGTTGTCTTTGCATCAGGTGGTACCGGATTTGACCCTATGACACCCCAAATAGTGTCAGCTATATCAATGGATGATCAGTTGAAAATGTTCAAAGAGTATATAGGGAAGCTCAAGGGCGTTGTTGGAGAAGAGAGAACCAAGTTCATCCTATCCAAGGCTCTAGTTCTGGTGGTTGCCGGCAGCGATGACATCGCCAACACCTATTTTGGCATTCGCATAAGACAAGCTCAGTATAGTGTTCCTGCTTACACTGACCTCATGGTCAACTCTGCATCTAGTTTCGTCAAGGAATTGTACAAACTTGGGGTGAGAAGGATTGGCGTGTTCAGTGCACCACCAATAGGATGTGTGCCTTCGCAAAGAACTTTGGGTGGAGGAATTCTGAGAGGTTGTGCAGAAGAGCTCAACGACGCGGCAAAGTTATTCAACATCAAACTGTCCAGCTCACTCAACTCTTTGAGCTCCGCCCTGTCCGGCAGTAAAATCATCTACGTGGATGTTTACAATCCACTGCTTGATATCATTCTCAACCCTGCCAAATATGGTTTTAAAGTTGTGGAGCAAGGTTGCTGTGGTACGGGGATTATAGAGGTAGCAGTGTTATGCAACAAGTACGATTCTACGTGTGAAAATCGATCTGATTATATATTTTGGGATAGTTATCATCCTACAGAACGAACTTACAACGTACTCATTCCTTCAATTATCAAAAAATATATTAACGACTTCTTAAGCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

41.43

Weight (kDa)

5.86

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
Acc36I ACCTGC 1 cut(s) 279
Acc65I GGTACC 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 422
AccII CGCG 1 cut(s) 815
AciI CCGC 3 cut(s) 170, 815, 867
AclWI GGATC 2 cut(s) 362, 375
AcsI RAATTY 1 cut(s) 780
AfaI GTAC 6 cut(s) 116, 424, 698, 979, 1016, 1092
AfiI CCNNNNNNNGG 3 cut(s) 254, 556, 743
AflII CTTAAG 1 cut(s) 1132
AflIII ACRYGT 1 cut(s) 1025
AgsI TTSAA 4 cut(s) 484, 493, 829, 1104
AluBI AGCT 7 cut(s) 145, 461, 511, 629, 805, 846, 864
AluI AGCT 7 cut(s) 145, 461, 511, 629, 805, 846, 864
Alw21I GWGCWC 3 cut(s) 736, 807, 866
Alw26I GTCTC 1 cut(s) 20
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 2 cut(s) 362, 375
Ama87I CYCGRG 1 cut(s) 221
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 3 cut(s) 145, 271, 582
ApoI RAATTY 1 cut(s) 780
AseI ATTAAT 1 cut(s) 14
Asp700I GAANNNNTTC 1 cut(s) 488
Asp718I GGTACC 1 cut(s) 422
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 3 cut(s) 151, 184, 721
AvaI CYCGRG 1 cut(s) 221
BaeGI GKGCMC 2 cut(s) 312, 736
BamHI GGATCC 1 cut(s) 367
BanI GGYRCC 1 cut(s) 422
BanII GRGCYC 2 cut(s) 807, 866
Bbv12I GWGCWC 3 cut(s) 736, 807, 866
BbvI GCAGC 3 cut(s) 132, 283, 594
BclI TGATCA 1 cut(s) 475
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 3 cut(s) 260, 563, 678
BfmI CTRYAG 2 cut(s) 120, 1071
BfrI CTTAAG 1 cut(s) 1132
BfuAI ACCTGC 1 cut(s) 279
BglII AGATCT 1 cut(s) 388
BisI GCNGC 4 cut(s) 146, 272, 583, 816
BlsI GCNGC 4 cut(s) 147, 273, 584, 817
Bme1390I CCNGG 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 369, 424
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 2 cut(s) 422, 683
BpuEI CTTGAG 2 cut(s) 369, 497
BpuMI CCSGG 1 cut(s) 70
Bsa29I ATCGAT 1 cut(s) 1036
BsaAI YACGTR 2 cut(s) 895, 1026
BsaBI GATNNNNATC 2 cut(s) 372, 1062
BsaJI CCNNGG 1 cut(s) 555
BsaWI WCCGGW 2 cut(s) 398, 425
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 556, 743
Bse118I RCCGGY 2 cut(s) 355, 578
Bse1I ACTGG 3 cut(s) 112, 141, 289
Bse3DI GCAATG 1 cut(s) 304
Bse8I GATNNNNATC 2 cut(s) 372, 1062
BseCI ATCGAT 1 cut(s) 1036
BseDI CCNNGG 1 cut(s) 555
BseGI GGATG 5 cut(s) 478, 546, 752, 904, 1066
BseJI GATNNNNATC 2 cut(s) 372, 1062
BseLI CCNNNNNNNGG 3 cut(s) 254, 556, 743
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 3 cut(s) 327, 644, 776
BseNI ACTGG 3 cut(s) 112, 141, 289
BseSI GKGCMC 2 cut(s) 312, 736
BseXI GCAGC 3 cut(s) 132, 283, 594
BsgI GTGCAG 1 cut(s) 816
Bsh1236I CGCG 1 cut(s) 815
BshNI GGYRCC 1 cut(s) 422
BshVI ATCGAT 1 cut(s) 1036
BsiHKAI GWGCWC 3 cut(s) 736, 807, 866
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 7 cut(s) 70, 167, 356, 399, 426, 579, 876
BslFI GGGAC 2 cut(s) 52, 147
BslI CCNNNNNNNGG 3 cut(s) 254, 556, 743
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 2 cut(s) 52, 147
BsmI GAATGC 1 cut(s) 612
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 4 cut(s) 312, 736, 807, 866
Bsp1407I TGTACA 1 cut(s) 696
Bsp143I GATC 7 cut(s) 174, 192, 217, 367, 388, 475, 1037
BspACI CCGC 3 cut(s) 170, 815, 867
BspCNI CTCAG 3 cut(s) 326, 643, 777
BspDI ATCGAT 1 cut(s) 1036
BspFNI CGCG 1 cut(s) 815
BspLI GGNNCC 2 cut(s) 369, 424
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 2 cut(s) 362, 375
BspQI GCTCTTC 1 cut(s) 795
BspT107I GGYRCC 1 cut(s) 422
BspTI CTTAAG 1 cut(s) 1132
BsrDI GCAATG 1 cut(s) 304
BsrFI RCCGGY 2 cut(s) 355, 578
BsrGI TGTACA 1 cut(s) 696
BsrI ACTGG 3 cut(s) 112, 141, 289
BssAI RCCGGY 2 cut(s) 355, 578
BssECI CCNNGG 1 cut(s) 555
BssMI GATC 7 cut(s) 174, 192, 217, 367, 388, 475, 1037
BssT1I CCWWGG 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 163, 840
Bst6I CTCTTC 3 cut(s) 43, 525, 795
BstAFI CTTAAG 1 cut(s) 1132
BstAUI TGTACA 1 cut(s) 696
BstBAI YACGTR 2 cut(s) 895, 1026
BstC8I GCNNGC 2 cut(s) 357, 580
BstDEI CTNAG 3 cut(s) 313, 630, 785
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 5 cut(s) 478, 546, 752, 904, 1066
BstFNI CGCG 1 cut(s) 815
BstKTI GATC 7 cut(s) 177, 195, 220, 370, 391, 478, 1040
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 7 cut(s) 174, 192, 217, 367, 388, 475, 1037
BstMWI GCNNNNNNNGC 2 cut(s) 277, 294
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 2 cut(s) 120, 1071
BstSLI GKGCMC 2 cut(s) 312, 736
BstUI CGCG 1 cut(s) 815
BstV1I GCAGC 3 cut(s) 132, 283, 594
BstX2I RGATCY 2 cut(s) 367, 388
BstYI RGATCY 2 cut(s) 367, 388
Bsu15I ATCGAT 1 cut(s) 1036
BsuTUI ATCGAT 1 cut(s) 1036
BtgZI GCGATG 2 cut(s) 577, 600
BtsCI GGATG 5 cut(s) 478, 546, 752, 904, 1066
BtsI GCAGTG 2 cut(s) 911, 1005
BtsIMutI CAGTG 4 cut(s) 651, 736, 911, 1005
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 2 cut(s) 357, 580
Cfr10I RCCGGY 2 cut(s) 355, 578
ClaI ATCGAT 1 cut(s) 1036
CseI GACGC 2 cut(s) 315, 821
Csp6I GTAC 6 cut(s) 115, 423, 697, 978, 1015, 1091
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 2 cut(s) 196, 661
CviQI GTAC 6 cut(s) 115, 423, 697, 978, 1015, 1091
DdeI CTNAG 3 cut(s) 313, 630, 785
DpnI GATC 7 cut(s) 176, 194, 219, 369, 390, 477, 1039
DpnII GATC 7 cut(s) 174, 192, 217, 367, 388, 475, 1037
DraI TTTAAA 1 cut(s) 952
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
Eam1104I CTCTTC 3 cut(s) 43, 525, 795
EarI CTCTTC 3 cut(s) 43, 525, 795
EciI GGCGGA 1 cut(s) 856
Ecl136II GAGCTC 2 cut(s) 805, 864
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 2 cut(s) 807, 866
Eco32I GATATC 1 cut(s) 922
Eco53kI GAGCTC 2 cut(s) 805, 864
Eco88I CYCGRG 1 cut(s) 221
EcoICRI GAGCTC 2 cut(s) 805, 864
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoRI GAATTC 1 cut(s) 780
EcoRV GATATC 1 cut(s) 922
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 2 cut(s) 807, 866
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 2 cut(s) 199, 664
FaqI GGGAC 2 cut(s) 52, 147
FatI CATG 2 cut(s) 195, 660
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 4 cut(s) 146, 272, 583, 816
FokI GGATG 5 cut(s) 485, 533, 759, 911, 1053
FriOI GRGCYC 2 cut(s) 807, 866
Fsp4HI GCNGC 4 cut(s) 146, 272, 583, 816
FspBI CTAG 3 cut(s) 260, 563, 678
GluI GCNGC 4 cut(s) 146, 272, 583, 816
HapII CCGG 7 cut(s) 70, 167, 356, 399, 426, 579, 876
HgaI GACGC 2 cut(s) 315, 821
Hin1II CATG 2 cut(s) 199, 664
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 4 cut(s) 188, 224, 339, 1019
HpaII CCGG 7 cut(s) 70, 167, 356, 399, 426, 579, 876
HphI GGTGA 3 cut(s) 151, 184, 721
Hpy166II GTNNAC 3 cut(s) 667, 734, 904
Hpy188I TCNGA 5 cut(s) 316, 331, 344, 786, 1042
Hpy188III TCNNGA 3 cut(s) 9, 221, 386
Hpy8I GTNNAC 3 cut(s) 667, 734, 904
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 4 cut(s) 60, 708, 765, 1110
HpyCH4III ACNGT 2 cut(s) 163, 840
HpyCH4IV ACGT 3 cut(s) 894, 1025, 1089
HpyCH4V TGCA 6 cut(s) 240, 413, 674, 734, 797, 1008
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 294
HpyF3I CTNAG 3 cut(s) 313, 630, 785
HpySE526I ACGT 3 cut(s) 894, 1025, 1089
Hsp92II CATG 2 cut(s) 199, 664
KpnI GGTACC 1 cut(s) 426
KroI GCCGGC 2 cut(s) 355, 578
KroNI GCCGGC 2 cut(s) 357, 580
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 7 cut(s) 174, 192, 217, 367, 388, 475, 1037
LguI GCTCTTC 1 cut(s) 795
LmnI GCTCC 2 cut(s) 869, 961
Lsp1109I GCAGC 3 cut(s) 132, 283, 594
LweI GCATC 2 cut(s) 422, 683
MaeI CTAG 3 cut(s) 260, 563, 678
MaeII ACGT 3 cut(s) 894, 1025, 1089
MaeIII GTNAC 1 cut(s) 225
MalI GATC 7 cut(s) 176, 194, 219, 369, 390, 477, 1039
MboI GATC 7 cut(s) 174, 192, 217, 367, 388, 475, 1037
MboII GAAGA 5 cut(s) 24, 30, 33, 542, 812
MflI RGATCY 2 cut(s) 367, 388
MhlI GDGCHC 4 cut(s) 312, 736, 807, 866
MluCI AATT 6 cut(s) 15, 241, 332, 692, 780, 1104
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 505
MroNI GCCGGC 2 cut(s) 355, 578
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 4 cut(s) 14, 951, 1122, 1133
MslI CAYNNNNRTG 1 cut(s) 893
MspCI CTTAAG 1 cut(s) 1132
MspI CCGG 7 cut(s) 70, 167, 356, 399, 426, 579, 876
MspR9I CCNGG 1 cut(s) 70
Mva1269I GAATGC 1 cut(s) 612
MvnI CGCG 1 cut(s) 815
MwoI GCNNNNNNNGC 2 cut(s) 277, 294
NaeI GCCGGC 2 cut(s) 357, 580
NciI CCSGG 1 cut(s) 70
NdeII GATC 7 cut(s) 174, 192, 217, 367, 388, 475, 1037
NgoMIV GCCGGC 2 cut(s) 355, 578
NlaIII CATG 2 cut(s) 199, 664
NlaIV GGNNCC 2 cut(s) 369, 424
NmuCI GTSAC 1 cut(s) 225
PaeR7I CTCGAG 1 cut(s) 221
PciSI GCTCTTC 1 cut(s) 795
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 612
PdiI GCCGGC 2 cut(s) 357, 580
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 3 cut(s) 188, 339, 1019
PkrI GCNGC 4 cut(s) 147, 273, 584, 817
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Ppu21I YACGTR 2 cut(s) 895, 1026
PshBI ATTAAT 1 cut(s) 14
Psp124BI GAGCTC 2 cut(s) 807, 866
PspN4I GGNNCC 2 cut(s) 369, 424
PsuI RGATCY 2 cut(s) 367, 388
RsaI GTAC 6 cut(s) 116, 424, 698, 979, 1016, 1092
RsaNI GTAC 6 cut(s) 115, 423, 697, 978, 1015, 1091
RseI CAYNNNNRTG 1 cut(s) 893
SacI GAGCTC 2 cut(s) 807, 866
SapI GCTCTTC 1 cut(s) 795
SaqAI TTAA 4 cut(s) 14, 951, 1122, 1133
SatI GCNGC 4 cut(s) 146, 272, 583, 816
Sau3AI GATC 7 cut(s) 174, 192, 217, 367, 388, 475, 1037
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 70
SduI GDGCHC 4 cut(s) 312, 736, 807, 866
SfaNI GCATC 2 cut(s) 422, 683
SfcI CTRYAG 2 cut(s) 120, 1071
Sfr274I CTCGAG 1 cut(s) 221
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 893
SmlI CTYRAG 4 cut(s) 221, 384, 512, 1132
SmoI CTYRAG 4 cut(s) 221, 384, 512, 1132
Sse9I AATT 6 cut(s) 15, 241, 332, 692, 780, 1104
SsiI CCGC 3 cut(s) 170, 815, 867
SspMI CTAG 3 cut(s) 260, 563, 678
SstI GAGCTC 2 cut(s) 807, 866
StyD4I CCNGG 1 cut(s) 68
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 2 cut(s) 163, 840
TaiI ACGT 3 cut(s) 897, 1028, 1092
TaqI TCGA 3 cut(s) 191, 222, 1036
TasI AATT 6 cut(s) 15, 241, 332, 692, 780, 1104
TatI WGTACW 1 cut(s) 696
TauI GCSGC 1 cut(s) 818
TfiI GAWTC 3 cut(s) 188, 339, 1019
Tru1I TTAA 4 cut(s) 14, 951, 1122, 1133
Tru9I TTAA 4 cut(s) 14, 951, 1122, 1133
TscAI CASTG 4 cut(s) 658, 736, 918, 1005
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 3 cut(s) 145, 271, 582
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 1 cut(s) 535
TspRI CASTG 4 cut(s) 658, 736, 918, 1005
Vha464I CTTAAG 1 cut(s) 1132
VneI GTGCAC 1 cut(s) 732
VspI ATTAAT 1 cut(s) 14
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 1 cut(s) 780
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 3 cut(s) 260, 563, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.