RLG00000011087

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001158
Physical Location & Seq
Reverse (-)
6673575 .. 6675685
2111 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011087

Sequence Viewer

Length: 1008 bp
ATGAAGCTTAAAATACCAGCGAACGTAAGTGTTCCGGCAGTGATTGTGTTTGGAGATTCAATAGTTGATACGGGCAACAACAACAACAACTTCATAACATTTGCTCGCTGCAATTTCCTCCCTTATGGCATAGACTTCCAAGGTGGACTTCCAACCGGACGATACTCAAATGGAAAGGTTCCTGCTGACCTTATAGTGGAAGCATTGGGAATTAAGGAGCTGTTGCCCCCATATATGGATCCGTCCCTAAAGCCTAATGATCTCCTTACTGGTATTAACTTGGCTGCTGGAGGTGTAGGTTATGACCCTTTGACATCAGAAGTCGCGGCAGTTGAATCACTGTCCGATCAGCTGGAACAGTTCAAAAGCTACATAGAAAAGCTGAAAGGAATCGTCGGAGAAAAGAGAACAAACTTCATCATATCAAATAGTTTGATTTTAGTGGTAGCAGGAAGCAACGACATTTCGAATACCTATTTTCTTGCTGGTATCAGGAAATTGCATTTCGATGTTCCTTCTTACACCGACTTCATGCTCAACCATGCTTCGCAATTCGTCAAGGAATTATATGGATTGGGCGCACGAAGAATAGGCGTTTTTGGTGTACCAGCTATTGGGTGTGTGCCATCCCAAAGAACAATTGGAGGAGGCATCGGAAGACGTTGTGCCGAGAATCAAAACCAAGCAGCGGAGCTCTTCAACTCCAAACTATCTGCAGAGACGAATCACCTTAGACACACACTCCCCAACTCAAAGTTCGTTTACATAGACGTCTACAACCCATTACTTGATATCATCACAAACTCTAATAAATACGGGTTTGAAGTTGCAGACAAAGGGTGTTGTGGTACTGGTTTGCTGGAAGTAACAAAACTGTGCAACCAGTTTCAGCGAACCTGTACGGACACCTCCAAGTATGTCTTTTGGGATAGTTACCATCCAACTGAAAGAGCATACAGGATCCTTGTGCAGTCAGTCCTAACGAAATCTGCAAGCGGTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

336

Amino Acids

36.68

Weight (kDa)

7.56

Isoelectric Point (pI)

19.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 14 - 324 2.1e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 774
AccB7I CCANNNNNTGG 1 cut(s) 614
AccI GTMKAC 1 cut(s) 774
AccII CGCG 1 cut(s) 326
AciI CCGC 3 cut(s) 326, 689, 996
AclWI GGATC 4 cut(s) 233, 246, 955, 968
AcyI GRCGYC 1 cut(s) 771
AfaI GTAC 3 cut(s) 606, 850, 901
AfiI CCNNNNNNNGG 4 cut(s) 196, 235, 614, 688
AgsI TTSAA 5 cut(s) 60, 335, 364, 700, 824
AjuI GAANNNNNNNTTGG 4 cut(s) 132, 164, 740, 772
AluBI AGCT 7 cut(s) 7, 220, 352, 369, 382, 611, 694
AluI AGCT 7 cut(s) 7, 220, 352, 369, 382, 611, 694
Alw21I GWGCWC 1 cut(s) 696
Alw26I GTCTC 1 cut(s) 713
AlwI GGATC 4 cut(s) 233, 246, 955, 968
ApeKI GCWGC 3 cut(s) 108, 284, 686
AspLEI GCGC 1 cut(s) 581
AsuHPI GGTGA 1 cut(s) 719
AsuII TTCGAA 1 cut(s) 467
BamHI GGATCC 2 cut(s) 238, 960
BanII GRGCYC 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 1 cut(s) 696
BbvI GCAGC 3 cut(s) 95, 271, 698
BccI CCATC 2 cut(s) 634, 945
BcoDI GTCTC 1 cut(s) 713
BfmI CTRYAG 1 cut(s) 714
BisI GCNGC 4 cut(s) 109, 285, 327, 687
BlsI GCNGC 4 cut(s) 110, 286, 328, 688
BmiI GGNNCC 3 cut(s) 180, 240, 962
BmsI GCATC 1 cut(s) 660
BpiI GAAGAC 1 cut(s) 664
BpmI CTGGAG 1 cut(s) 309
Bpu14I TTCGAA 1 cut(s) 467
BsaHI GRCGYC 1 cut(s) 771
BsaJI CCNNGG 1 cut(s) 139
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 726, 756
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 235, 614, 688
Bse1I ACTGG 3 cut(s) 274, 856, 883
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 2 cut(s) 626, 937
BseLI CCNNNNNNNGG 4 cut(s) 196, 235, 614, 688
BseNI ACTGG 3 cut(s) 274, 856, 883
BseRI GAGGAG 1 cut(s) 660
BseXI GCAGC 3 cut(s) 95, 271, 698
BsgI GTGCAG 1 cut(s) 989
Bsh1236I CGCG 1 cut(s) 326
BsiHKAI GWGCWC 1 cut(s) 696
BsiSI CCGG 2 cut(s) 35, 156
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 4 cut(s) 196, 235, 614, 688
BsmAI GTCTC 1 cut(s) 713
BsmBI CGTCTC 1 cut(s) 713
BsmFI GGGAC 1 cut(s) 229
Bsp119I TTCGAA 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 696
Bsp143I GATC 4 cut(s) 238, 259, 346, 960
BspACI CCGC 3 cut(s) 326, 689, 996
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 3 cut(s) 180, 240, 962
BspMAI CTGCAG 1 cut(s) 718
BspPI GGATC 4 cut(s) 233, 246, 955, 968
BspQI GCTCTTC 1 cut(s) 701
BspT104I TTCGAA 1 cut(s) 467
BsrI ACTGG 3 cut(s) 274, 856, 883
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 4 cut(s) 238, 259, 346, 960
BssNI GRCGYC 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 139
Bst4CI ACNGT 3 cut(s) 342, 360, 876
Bst6I CTCTTC 1 cut(s) 701
BstACI GRCGYC 1 cut(s) 771
BstBI TTCGAA 1 cut(s) 467
BstC8I GCNNGC 2 cut(s) 106, 994
BstDEI CTNAG 1 cut(s) 731
BstF5I GGATG 2 cut(s) 626, 937
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 581
BstKTI GATC 4 cut(s) 241, 262, 349, 963
BstMAI GTCTC 1 cut(s) 713
BstMBI GATC 4 cut(s) 238, 259, 346, 960
BstSFI CTRYAG 1 cut(s) 714
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 3 cut(s) 95, 271, 698
BstV2I GAAGAC 1 cut(s) 664
BstX2I RGATCY 2 cut(s) 238, 960
BstYI RGATCY 2 cut(s) 238, 960
BtsCI GGATG 2 cut(s) 626, 937
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 45, 338
Cac8I GCNNGC 2 cut(s) 106, 994
CfoI GCGC 1 cut(s) 581
Csp6I GTAC 3 cut(s) 605, 849, 900
CviAII CATG 2 cut(s) 532, 542
CviJI RGCY 9 cut(s) 7, 220, 253, 284, 352, 369, 382, 611, 694
CviKI_1 RGCY 9 cut(s) 7, 220, 253, 284, 352, 369, 382, 611, 694
CviQI GTAC 3 cut(s) 605, 849, 900
DdeI CTNAG 1 cut(s) 731
DpnI GATC 4 cut(s) 240, 261, 348, 962
DpnII GATC 4 cut(s) 238, 259, 346, 960
Eam1104I CTCTTC 1 cut(s) 701
EarI CTCTTC 1 cut(s) 701
Ecl136II GAGCTC 1 cut(s) 694
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 696
Eco32I GATATC 1 cut(s) 793
Eco53kI GAGCTC 1 cut(s) 694
EcoICRI GAGCTC 1 cut(s) 694
EcoRV GATATC 1 cut(s) 793
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 139
Esp3I CGTCTC 1 cut(s) 713
FaeI CATG 2 cut(s) 535, 545
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 905, 937
FaqI GGGAC 1 cut(s) 229
FatI CATG 2 cut(s) 531, 541
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 4 cut(s) 109, 285, 327, 687
FokI GGATG 2 cut(s) 613, 924
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 4 cut(s) 109, 285, 327, 687
GlaI GCGC 1 cut(s) 580
GluI GCNGC 4 cut(s) 109, 285, 327, 687
GsuI CTGGAG 1 cut(s) 309
HapII CCGG 2 cut(s) 35, 156
HhaI GCGC 1 cut(s) 581
Hin1I GRCGYC 1 cut(s) 771
Hin1II CATG 2 cut(s) 535, 545
Hin6I GCGC 1 cut(s) 579
HinP1I GCGC 1 cut(s) 579
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 5 cut(s) 56, 335, 390, 673, 724
HpaII CCGG 2 cut(s) 35, 156
HphI GGTGA 1 cut(s) 719
Hpy166II GTNNAC 4 cut(s) 146, 605, 763, 775
Hpy188I TCNGA 4 cut(s) 319, 346, 398, 656
Hpy188III TCNNGA 1 cut(s) 493
Hpy8I GTNNAC 4 cut(s) 146, 605, 763, 775
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 1 cut(s) 525
HpyCH4III ACNGT 3 cut(s) 342, 360, 876
HpyCH4IV ACGT 3 cut(s) 24, 661, 771
HpyCH4V TGCA 7 cut(s) 111, 502, 716, 830, 879, 970, 992
HpyF3I CTNAG 1 cut(s) 731
HpySE526I ACGT 3 cut(s) 24, 661, 771
Hsp92I GRCGYC 1 cut(s) 771
Hsp92II CATG 2 cut(s) 535, 545
HspAI GCGC 1 cut(s) 579
Kzo9I GATC 4 cut(s) 238, 259, 346, 960
LguI GCTCTTC 1 cut(s) 701
LmnI GCTCC 2 cut(s) 217, 691
Lsp1109I GCAGC 3 cut(s) 95, 271, 698
LweI GCATC 1 cut(s) 660
MaeII ACGT 3 cut(s) 24, 661, 771
MaeIII GTNAC 2 cut(s) 865, 932
MalI GATC 4 cut(s) 240, 261, 348, 962
MboI GATC 4 cut(s) 238, 259, 346, 960
MboII GAAGA 3 cut(s) 597, 669, 688
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 2 cut(s) 238, 960
MhlI GDGCHC 1 cut(s) 696
MluCI AATT 6 cut(s) 112, 210, 497, 551, 563, 639
MmeI TCCRAC 3 cut(s) 176, 376, 965
MnlI CCTC 5 cut(s) 128, 284, 638, 641, 919
MseI TTAA 3 cut(s) 9, 213, 276
MslI CAYNNNNRTG 1 cut(s) 507
MspA1I CMGCKG 2 cut(s) 352, 689
MspI CCGG 2 cut(s) 35, 156
MunI CAATTG 1 cut(s) 639
MvnI CGCG 1 cut(s) 326
NdeII GATC 4 cut(s) 238, 259, 346, 960
NlaIII CATG 2 cut(s) 535, 545
NlaIV GGNNCC 3 cut(s) 180, 240, 962
NmeAIII GCCGAG 1 cut(s) 694
NspV TTCGAA 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 701
PfeI GAWTC 5 cut(s) 56, 335, 390, 673, 724
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 4 cut(s) 110, 286, 328, 688
Psp124BI GAGCTC 1 cut(s) 696
PspN4I GGNNCC 3 cut(s) 180, 240, 962
PstI CTGCAG 1 cut(s) 718
PsuI RGATCY 2 cut(s) 238, 960
PvuII CAGCTG 1 cut(s) 352
RsaI GTAC 3 cut(s) 606, 850, 901
RsaNI GTAC 3 cut(s) 605, 849, 900
RseI CAYNNNNRTG 1 cut(s) 507
SacI GAGCTC 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 701
SaqAI TTAA 3 cut(s) 9, 213, 276
SatI GCNGC 4 cut(s) 109, 285, 327, 687
Sau3AI GATC 4 cut(s) 238, 259, 346, 960
SduI GDGCHC 1 cut(s) 696
SfaNI GCATC 1 cut(s) 660
SfcI CTRYAG 1 cut(s) 714
SfuI TTCGAA 1 cut(s) 467
SmiMI CAYNNNNRTG 1 cut(s) 507
Sse9I AATT 6 cut(s) 112, 210, 497, 551, 563, 639
SsiI CCGC 3 cut(s) 326, 689, 996
SstI GAGCTC 1 cut(s) 696
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 3 cut(s) 342, 360, 876
TaiI ACGT 3 cut(s) 27, 664, 774
TaqI TCGA 2 cut(s) 467, 507
TasI AATT 6 cut(s) 112, 210, 497, 551, 563, 639
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 5 cut(s) 56, 335, 390, 673, 724
Tru1I TTAA 3 cut(s) 9, 213, 276
Tru9I TTAA 3 cut(s) 9, 213, 276
TscAI CASTG 2 cut(s) 45, 345
TseI GCWGC 3 cut(s) 108, 284, 686
TspDTI ATGAA 4 cut(s) 17, 82, 406, 520
TspGWI ACGGA 2 cut(s) 231, 917
TspRI CASTG 2 cut(s) 45, 345
Van91I CCANNNNNTGG 1 cut(s) 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmiI GTMKAC 1 cut(s) 774
ZraI GACGTC 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.