AT3G43570

Contains the following InterPro domains Lipase, GDSL, active site (InterPro IPR008265), Lipase, GDSL (InterPro IPR001087)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001158
Physical Location & Seq
Forward (+)
15473345 .. 15474765
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G43570.1

Sequence Viewer

Length: 963 bp
ATGAAGATTCAAATTATATGGCTCACTTTGGTTTTAATCGTCGTTGAAGCTAATGCAGTCAAACAAGGGAAAAACGCAACGATCCCGGCCCTAATAGTTTTTGGAGATTCAATAATGGATACAGGAAATAACAATAATCTTCCCACTCTTCTTAAATGTAACTTCCCTCCATACGGTAAAGATTATCCTGGAGGCTTTGCCACTGGAAGATTTTCTGATGGAAGAGTTCCTTCTGATCTTATTGCGGAAAAAATAGGATTGGCTAAGACATTACCAGCATATATGAATCCATATTTGAAGCCAGAGGATCTTCTTAAAGGTGTAACATTTGCATCAGGAGGAACTGGTTATGATCCATTGACAGCAAAAATTATGTCAGTGATATCAGTGTGGGATCAACTCATATATTTCAAAGAATATATATCAAAGATCAAGAGGCATTTCGGAGAAGAAAAAGCAAAAGATATCTTGGAACATAGTTTCTTTCTTGTGGTGTCTAGTAGCAATGACCTTGCTCACACTTATTTAGCTCAAGCTCATAGATATGATCGTACCTCTTATGCCAATTTCTTGGCTGACTCTGCTGTTCATTTCGTGAGAGAATTACACAAGCTTGGAGCTCAAAAAATTGGAGTGTTTAGTGCAGTCCCTGTTGGTTGTGTTCCACTCCAAAGAACAGTTTTTGGAGATAAAGAGTTGGATGGTGTTATCCTTTACATTAATGTTTATGATACTCTTTTCGACATGATCCAACACCCTAAAAAATACGGTTTTGAAGTGGCTGATAGAGGATGTTGCGGTAAAGGACTGCTCACGATCTCCTATTTATGTAACTCATTGAACCAATTTACGTGTTCTAACTCTTCAGCTTATATATTTTGGGATAGCTATCATCCAAGTAAAAGAGCTTATCAAGTTATTGTCGACAATTTACTTGACAAATATTTGAGCAAAGTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

320

Amino Acids

35.76

Weight (kDa)

8.36

Isoelectric Point (pI)

20.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 310 3.7e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 924
AciI CCGC 2 cut(s) 245, 798
AclWI GGATC 5 cut(s) 76, 315, 347, 402, 742
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 851
AgsI TTSAA 7 cut(s) 11, 47, 111, 298, 412, 776, 841
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 8 cut(s) 50, 530, 536, 613, 620, 869, 888, 908
AluI AGCT 8 cut(s) 50, 530, 536, 613, 620, 869, 888, 908
Alw21I GWGCWC 1 cut(s) 622
AlwI GGATC 5 cut(s) 76, 315, 347, 402, 742
AlwNI CAGNNNCTG 1 cut(s) 650
AoxI GGCC 1 cut(s) 87
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
AseI ATTAAT 1 cut(s) 720
Asp700I GAANNNNTTC 1 cut(s) 211
AspS9I GGNCC 1 cut(s) 88
AsuC2I CCSGG 1 cut(s) 86
BanII GRGCYC 1 cut(s) 622
Bbv12I GWGCWC 1 cut(s) 622
BccI CCATC 2 cut(s) 212, 695
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 1 cut(s) 112
BcnI CCSGG 1 cut(s) 86
BfaI CTAG 1 cut(s) 498
BfuI GTATCC 1 cut(s) 112
Bme1390I CCNGG 2 cut(s) 86, 189
BmgT120I GGNCC 1 cut(s) 88
BmrFI CCNGG 2 cut(s) 86, 189
BmsI GCATC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 516
BpuMI CCSGG 1 cut(s) 86
BsaAI YACGTR 1 cut(s) 852
BsaBI GATNNNNATC 1 cut(s) 888
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse1I ACTGG 2 cut(s) 208, 349
Bse3DI GCAATG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 888
BseBI CCWGG 1 cut(s) 189
BseGI GGATG 3 cut(s) 706, 797, 892
BseJI GATNNNNATC 1 cut(s) 888
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMI GCAATG 1 cut(s) 511
BseNI ACTGG 2 cut(s) 208, 349
BsgI GTGCAG 1 cut(s) 663
BshFI GGCC 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 1 cut(s) 86
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 632
BsnI GGCC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 9 cut(s) 81, 235, 307, 352, 394, 429, 547, 747, 816
BspACI CCGC 2 cut(s) 245, 798
BspANI GGCC 1 cut(s) 89
BspPI GGATC 5 cut(s) 76, 315, 347, 402, 742
BsrDI GCAATG 1 cut(s) 511
BsrI ACTGG 2 cut(s) 208, 349
BssMI GATC 9 cut(s) 81, 235, 307, 352, 394, 429, 547, 747, 816
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 3 cut(s) 176, 679, 770
Bst6I CTCTTC 3 cut(s) 153, 217, 868
BstBAI YACGTR 1 cut(s) 852
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 706, 797, 892
BstKTI GATC 9 cut(s) 84, 238, 310, 355, 397, 432, 550, 750, 819
BstMBI GATC 9 cut(s) 81, 235, 307, 352, 394, 429, 547, 747, 816
BstMWI GCNNNNNNNGC 1 cut(s) 581
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 2 cut(s) 84, 187
BstX2I RGATCY 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 571
BstYI RGATCY 1 cut(s) 307
BsuI GTATCC 1 cut(s) 112
BsuRI GGCC 1 cut(s) 89
BtsCI GGATG 3 cut(s) 706, 797, 892
BtsIMutI CAGTG 3 cut(s) 201, 384, 393
CaiI CAGNNNCTG 1 cut(s) 650
Cfr13I GGNCC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 552
CviAII CATG 1 cut(s) 745
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 1 cut(s) 264
DpnI GATC 9 cut(s) 83, 237, 309, 354, 396, 431, 549, 749, 818
DpnII GATC 9 cut(s) 81, 235, 307, 352, 394, 429, 547, 747, 816
Eam1104I CTCTTC 3 cut(s) 153, 217, 868
EarI CTCTTC 3 cut(s) 153, 217, 868
Ecl136II GAGCTC 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 622
Eco32I GATATC 2 cut(s) 384, 466
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 849
EcoICRI GAGCTC 1 cut(s) 620
EcoRII CCWGG 1 cut(s) 187
EcoRV GATATC 2 cut(s) 384, 466
EcoT38I GRGCYC 1 cut(s) 622
FaeI CATG 1 cut(s) 748
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 632
FatI CATG 1 cut(s) 744
FblI GTMKAC 1 cut(s) 924
FokI GGATG 3 cut(s) 713, 804, 879
FriOI GRGCYC 1 cut(s) 622
FspBI CTAG 1 cut(s) 498
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 1 cut(s) 89
HapII CCGG 1 cut(s) 86
Hin1II CATG 1 cut(s) 748
HincII GTYRAC 1 cut(s) 925
HindII GTYRAC 1 cut(s) 925
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 4 cut(s) 7, 107, 286, 578
HpaII CCGG 1 cut(s) 86
Hpy166II GTNNAC 1 cut(s) 925
Hpy188I TCNGA 3 cut(s) 217, 235, 446
Hpy188III TCNNGA 4 cut(s) 336, 433, 595, 814
Hpy8I GTNNAC 1 cut(s) 925
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 240
HpyCH4III ACNGT 3 cut(s) 176, 679, 770
HpyCH4IV ACGT 1 cut(s) 851
HpyCH4V TGCA 3 cut(s) 56, 332, 644
HpyF10VI GCNNNNNNNGC 1 cut(s) 581
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 851
Hsp92II CATG 1 cut(s) 748
Kzo9I GATC 9 cut(s) 81, 235, 307, 352, 394, 429, 547, 747, 816
LmnI GCTCC 1 cut(s) 617
LweI GCATC 1 cut(s) 341
MaeI CTAG 1 cut(s) 498
MaeII ACGT 1 cut(s) 851
MaeIII GTNAC 3 cut(s) 158, 322, 830
MalI GATC 9 cut(s) 83, 237, 309, 354, 396, 431, 549, 749, 818
MboI GATC 9 cut(s) 81, 235, 307, 352, 394, 429, 547, 747, 816
MboII GAAGA 8 cut(s) 16, 131, 140, 219, 234, 302, 461, 855
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 7 cut(s) 12, 369, 565, 602, 627, 845, 928
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 2 cut(s) 678, 775
MnlI CCTC 7 cut(s) 177, 185, 298, 332, 429, 565, 782
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 4 cut(s) 35, 153, 315, 720
MslI CAYNNNNRTG 1 cut(s) 543
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 2 cut(s) 86, 189
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 581
NciI CCSGG 1 cut(s) 86
NdeII GATC 9 cut(s) 81, 235, 307, 352, 394, 429, 547, 747, 816
NlaIII CATG 1 cut(s) 748
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 3 cut(s) 7, 107, 286
PfoI TCCNGGA 1 cut(s) 187
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
Ppu21I YACGTR 1 cut(s) 852
PshBI ATTAAT 1 cut(s) 720
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspPI GGNCC 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
RseI CAYNNNNRTG 1 cut(s) 543
SacI GAGCTC 1 cut(s) 622
SalI GTCGAC 1 cut(s) 923
SaqAI TTAA 4 cut(s) 35, 153, 315, 720
Sau3AI GATC 9 cut(s) 81, 235, 307, 352, 394, 429, 547, 747, 816
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 2 cut(s) 86, 189
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 531
SmoI CTYRAG 1 cut(s) 531
Sse9I AATT 7 cut(s) 12, 369, 565, 602, 627, 845, 928
SsiI CCGC 2 cut(s) 245, 798
SspI AATATT 1 cut(s) 944
SspMI CTAG 1 cut(s) 498
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 84, 187
TaaI ACNGT 3 cut(s) 176, 679, 770
TaiI ACGT 1 cut(s) 854
TaqI TCGA 2 cut(s) 741, 924
TasI AATT 7 cut(s) 12, 369, 565, 602, 627, 845, 928
TfiI GAWTC 3 cut(s) 7, 107, 286
Tru1I TTAA 4 cut(s) 35, 153, 315, 720
Tru9I TTAA 4 cut(s) 35, 153, 315, 720
TscAI CASTG 3 cut(s) 208, 384, 393
TspDTI ATGAA 3 cut(s) 17, 299, 578
TspRI CASTG 3 cut(s) 208, 384, 393
VspI ATTAAT 1 cut(s) 720
XmiI GTMKAC 1 cut(s) 924
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.