RLG00000011088

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001158
Physical Location & Seq
Reverse (-)
6715339 .. 6717183
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011088

Sequence Viewer

Length: 1149 bp
ATGGGATTCCTCTTTATAGAAACCTTCTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTGGTTCCATAATGTCGTCTTCGAAGCAGCTCTTGATATTTGTTTGTAGTCTTGCTGTGGTTTTAGGGCAGTACCCCAGAAGCACAGAGGGTCGGGTGAATATACCACCAGGAAAGTCAATGCCGGCGGTTATAGTGTTCGGTGACTCGATCATGGACACCGGCAACAATAACAATCTTAAGTCTGCTGTTAAATGCAATTTCCCTCCATATGGTGAAAATTTCCCTGGAAAAGTTCCAACCGGTAGATTTGGCAATGGAAAAGTCCCCTCAGACTTCATAGCTCAAGAACTGGGAATCAAAGAGTTTGTGCCGGCATATGCGGATACTAGTCTAAAACCTGAAGATCTCAGCACTGGAGTCTGCTTCGCTTCAGGTGGCACCGGATATGACCCTCAGACCCCCGAATTAGTAGCAGTTATATCGCTATCGGAGCAGTTGAACATGTTCAAGGAGTATATAGGGAAGCTGAAAGCCAATTTTGGAGAGCAGAGAACAAACTTCATCCTCGCCAACAGTCTCTTTCTGGTGGTCGCCGGTAGCGATGACATTGCCAACACCTATTTCACTATTGGCATCAGAAAGGCAGAATATGACGTCCCTGCTTACACTGACCTTATGGTCAACTCTGCCACTAGTTTCCTTCAGGATTTGTATGCACTTGGGGCTAGAAGGTTCGGTGTTTTCAGTGCACCACCGATAGGGTGTGTACCGTCACAAAGAACTCTGGGTGGAGGAATACAAAGACAGTGTGCAGAAGAGTACAACGACGCAGCGAAGCTATTCAACTCTAAGCTGTCGTCCAAAATGCAGTCCTTGAATACAAAGCTGCCCAACAGCAGAATAGTTTATGTCGATGTTTACACCCCTCTACTTGATGTCATTCTTCACCCTGCCAAATACGGGTTTAAAGTGGCGGAGAAAGGTTGCTGTGGCACAGGGGCACTAGAGGTAGCCATATTATGCAACAAATTGTCTCCAACGTGTGAAAATCCGGCGGATCATGTATTTTGGGATAGTTATCACCCTACTGAACAAGCATACAGAGTACTCATTCCTCCAATTCTCCAAAAATACGTAAATCAATTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

383

Amino Acids

41.47

Weight (kDa)

6.34

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 62 - 369 1.6e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 674
AatII GACGTC 1 cut(s) 654
AccB1I GGYRCC 1 cut(s) 434
AciI CCGC 4 cut(s) 182, 377, 971, 1052
AclWI GGATC 1 cut(s) 1062
AcsI RAATTY 1 cut(s) 274
AcuI CTGAAG 3 cut(s) 411, 417, 683
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 4 cut(s) 128, 765, 818, 1104
AfiI CCNNNNNNNGG 3 cut(s) 266, 755, 957
AflII CTTAAG 1 cut(s) 233
AflIII ACRYGT 2 cut(s) 498, 1037
AgeI ACCGGT 1 cut(s) 296
AgsI TTSAA 4 cut(s) 496, 505, 841, 874
AhlI ACTAGT 2 cut(s) 383, 689
AjnI CCWGG 2 cut(s) 163, 280
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AluBI AGCT 6 cut(s) 85, 338, 523, 835, 850, 883
AluI AGCT 6 cut(s) 85, 338, 523, 835, 850, 883
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 2 cut(s) 578, 1035
Alw44I GTGCAC 1 cut(s) 744
AlwI GGATC 1 cut(s) 1062
ApaLI GTGCAC 1 cut(s) 744
ApeKI GCWGC 3 cut(s) 82, 827, 883
ApoI RAATTY 1 cut(s) 274
AsiGI ACCGGT 1 cut(s) 296
Asp700I GAANNNNTTC 2 cut(s) 500, 836
AsuHPI GGTGA 5 cut(s) 163, 209, 281, 935, 1070
AsuII TTCGAA 1 cut(s) 77
BaeGI GKGCMC 2 cut(s) 748, 1000
BanI GGYRCC 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 748
BbvI GCAGC 3 cut(s) 94, 839, 870
BcgI CGANNNNNNTGC 4 cut(s) 459, 493, 587, 621
BciT130I CCWGG 2 cut(s) 165, 282
BciVI GTATCC 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 578, 1035
BcuI ACTAGT 2 cut(s) 383, 689
BfaI CTAG 4 cut(s) 384, 690, 723, 1001
BfrI CTTAAG 1 cut(s) 233
BfuI GTATCC 1 cut(s) 373
BglII AGATCT 1 cut(s) 400
BisI GCNGC 3 cut(s) 83, 828, 884
BlsI GCNGC 3 cut(s) 84, 829, 885
BmcAI AGTACT 1 cut(s) 1104
Bme1390I CCNGG 2 cut(s) 165, 282
BmiI GGNNCC 2 cut(s) 61, 436
BmrFI CCNGG 2 cut(s) 165, 282
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 1 cut(s) 639
BmuI ACTGGG 1 cut(s) 356
BpiI GAAGAC 1 cut(s) 66
BpmI CTGGAG 1 cut(s) 432
Bpu14I TTCGAA 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 324
BsaAI YACGTR 1 cut(s) 1132
BsaBI GATNNNNATC 1 cut(s) 1074
BsaHI GRCGYC 1 cut(s) 651
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 2 cut(s) 296, 437
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 755, 957
Bse118I RCCGGY 5 cut(s) 178, 215, 296, 367, 590
Bse1I ACTGG 2 cut(s) 351, 415
Bse3DI GCAATG 2 cut(s) 316, 603
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 2 cut(s) 165, 282
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 558
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 3 cut(s) 266, 755, 957
BseMI GCAATG 2 cut(s) 316, 603
BseMII CTCAG 3 cut(s) 339, 418, 464
BseNI ACTGG 2 cut(s) 351, 415
BseSI GKGCMC 2 cut(s) 748, 1000
BseXI GCAGC 3 cut(s) 94, 839, 870
BsgI GTGCAG 1 cut(s) 828
BshNI GGYRCC 1 cut(s) 434
BshTI ACCGGT 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 748
BsiSI CCGG 7 cut(s) 179, 216, 297, 368, 438, 591, 1049
BslFI GGGAC 2 cut(s) 305, 638
BslI CCNNNNNNNGG 3 cut(s) 266, 755, 957
BsmAI GTCTC 2 cut(s) 578, 1035
BsmFI GGGAC 2 cut(s) 305, 638
Bsp119I TTCGAA 1 cut(s) 77
Bsp1286I GDGCHC 2 cut(s) 748, 1000
Bsp143I GATC 3 cut(s) 204, 400, 1054
BspACI CCGC 4 cut(s) 182, 377, 971, 1052
BspCNI CTCAG 3 cut(s) 338, 417, 463
BspLI GGNNCC 2 cut(s) 61, 436
BspPI GGATC 1 cut(s) 1062
BspT104I TTCGAA 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 434
BspTI CTTAAG 1 cut(s) 233
BsrDI GCAATG 2 cut(s) 316, 603
BsrFI RCCGGY 5 cut(s) 178, 215, 296, 367, 590
BsrI ACTGG 2 cut(s) 351, 415
BssAI RCCGGY 5 cut(s) 178, 215, 296, 367, 590
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 3 cut(s) 204, 400, 1054
BssNI GRCGYC 1 cut(s) 651
Bst2UI CCWGG 2 cut(s) 165, 282
Bst4CI ACNGT 3 cut(s) 572, 768, 804
Bst6I CTCTTC 2 cut(s) 34, 807
BstACI GRCGYC 1 cut(s) 651
BstAFI CTTAAG 1 cut(s) 233
BstBAI YACGTR 1 cut(s) 1132
BstBI TTCGAA 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 180, 369
BstDEI CTNAG 4 cut(s) 325, 404, 450, 846
BstF5I GGATG 1 cut(s) 558
BstKTI GATC 3 cut(s) 207, 403, 1057
BstMAI GTCTC 2 cut(s) 578, 1035
BstMBI GATC 3 cut(s) 204, 400, 1054
BstMWI GCNNNNNNNGC 2 cut(s) 487, 719
BstNI CCWGG 2 cut(s) 165, 282
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 2 cut(s) 163, 280
BstSLI GKGCMC 2 cut(s) 748, 1000
BstSNI TACGTA 1 cut(s) 1132
BstV1I GCAGC 3 cut(s) 94, 839, 870
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuI GTATCC 1 cut(s) 373
BtgZI GCGATG 1 cut(s) 612
BtsCI GGATG 1 cut(s) 558
BtsIMutI CAGTG 4 cut(s) 408, 663, 748, 809
Cac8I GCNNGC 2 cut(s) 180, 369
Cfr10I RCCGGY 5 cut(s) 178, 215, 296, 367, 590
CseI GACGC 1 cut(s) 833
Csp6I GTAC 4 cut(s) 127, 764, 817, 1103
CspAI ACCGGT 1 cut(s) 296
CviAII CATG 3 cut(s) 208, 499, 1058
CviJI RGCY 9 cut(s) 85, 338, 523, 530, 722, 835, 850, 883, 1010
CviKI_1 RGCY 9 cut(s) 85, 338, 523, 530, 722, 835, 850, 883, 1010
CviQI GTAC 4 cut(s) 127, 764, 817, 1103
DdeI CTNAG 4 cut(s) 325, 404, 450, 846
DpnI GATC 3 cut(s) 206, 402, 1056
DpnII GATC 3 cut(s) 204, 400, 1054
DraI TTTAAA 1 cut(s) 964
DrdI GACNNNNNNGTC 1 cut(s) 674
DseDI GACNNNNNNGTC 1 cut(s) 674
Eam1104I CTCTTC 2 cut(s) 34, 807
EarI CTCTTC 2 cut(s) 34, 807
EciI GGCGGA 2 cut(s) 986, 1067
Eco105I TACGTA 1 cut(s) 1132
Eco57I CTGAAG 3 cut(s) 411, 417, 683
EcoRII CCWGG 2 cut(s) 163, 280
FaeI CATG 3 cut(s) 211, 502, 1061
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 2 cut(s) 305, 638
FatI CATG 3 cut(s) 207, 498, 1057
FauNDI CATATG 2 cut(s) 265, 373
Fnu4HI GCNGC 3 cut(s) 83, 828, 884
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 3 cut(s) 83, 828, 884
FspBI CTAG 4 cut(s) 384, 690, 723, 1001
GluI GCNGC 3 cut(s) 83, 828, 884
GsuI CTGGAG 1 cut(s) 432
HapII CCGG 7 cut(s) 179, 216, 297, 368, 438, 591, 1049
HgaI GACGC 1 cut(s) 833
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 3 cut(s) 211, 502, 1061
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HinfI GANTC 4 cut(s) 6, 200, 351, 414
HpaII CCGG 7 cut(s) 179, 216, 297, 368, 438, 591, 1049
HphI GGTGA 5 cut(s) 163, 209, 281, 935, 1070
Hpy166II GTNNAC 4 cut(s) 679, 746, 764, 916
Hpy188I TCNGA 5 cut(s) 328, 453, 487, 635, 1148
Hpy188III TCNNGA 3 cut(s) 88, 341, 701
Hpy8I GTNNAC 4 cut(s) 679, 746, 764, 916
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 3 cut(s) 34, 707, 720
HpyCH4III ACNGT 3 cut(s) 572, 768, 804
HpyCH4IV ACGT 3 cut(s) 651, 1037, 1131
HpyCH4V TGCA 6 cut(s) 252, 713, 746, 809, 865, 1020
HpyF10VI GCNNNNNNNGC 2 cut(s) 487, 719
HpyF3I CTNAG 4 cut(s) 325, 404, 450, 846
HpySE526I ACGT 3 cut(s) 651, 1037, 1131
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 3 cut(s) 211, 502, 1061
KroI GCCGGC 2 cut(s) 178, 367
KroNI GCCGGC 2 cut(s) 180, 369
Kzo9I GATC 3 cut(s) 204, 400, 1054
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 3 cut(s) 94, 839, 870
LweI GCATC 1 cut(s) 639
MaeI CTAG 4 cut(s) 384, 690, 723, 1001
MaeII ACGT 3 cut(s) 651, 1037, 1131
MaeIII GTNAC 2 cut(s) 197, 768
MalI GATC 3 cut(s) 206, 402, 1056
MboI GATC 3 cut(s) 204, 400, 1054
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 2 cut(s) 748, 1000
MluCI AATT 7 cut(s) 253, 274, 461, 532, 1025, 1116, 1139
MlyI GAGTC 2 cut(s) 194, 423
MmeI TCCRAC 2 cut(s) 317, 1058
MroNI GCCGGC 2 cut(s) 178, 367
MroXI GAANNNNTTC 2 cut(s) 500, 836
MseI TTAA 3 cut(s) 234, 246, 963
MspCI CTTAAG 1 cut(s) 233
MspI CCGG 7 cut(s) 179, 216, 297, 368, 438, 591, 1049
MspR9I CCNGG 2 cut(s) 165, 282
MvaI CCWGG 2 cut(s) 165, 282
MwoI GCNNNNNNNGC 2 cut(s) 487, 719
NaeI GCCGGC 2 cut(s) 180, 369
NdeI CATATG 2 cut(s) 265, 373
NdeII GATC 3 cut(s) 204, 400, 1054
NgoMIV GCCGGC 2 cut(s) 178, 367
NlaIII CATG 3 cut(s) 211, 502, 1061
NlaIV GGNNCC 2 cut(s) 61, 436
NmuCI GTSAC 2 cut(s) 197, 768
NspI RCATGY 1 cut(s) 502
NspV TTCGAA 1 cut(s) 77
PciI ACATGT 1 cut(s) 498
PcsI WCGNNNNNNNCGW 1 cut(s) 594
PdiI GCCGGC 2 cut(s) 180, 369
PdmI GAANNNNTTC 2 cut(s) 500, 836
PfeI GAWTC 2 cut(s) 6, 351
PinAI ACCGGT 1 cut(s) 296
PkrI GCNGC 3 cut(s) 84, 829, 885
PleI GAGTC 2 cut(s) 194, 422
PpsI GAGTC 2 cut(s) 194, 422
Ppu21I YACGTR 1 cut(s) 1132
PscI ACATGT 1 cut(s) 498
Psp6I CCWGG 2 cut(s) 163, 280
PspGI CCWGG 2 cut(s) 163, 280
PspN4I GGNNCC 2 cut(s) 61, 436
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 4 cut(s) 128, 765, 818, 1104
RsaNI GTAC 4 cut(s) 127, 764, 817, 1103
SaqAI TTAA 3 cut(s) 234, 246, 963
SatI GCNGC 3 cut(s) 83, 828, 884
Sau3AI GATC 3 cut(s) 204, 400, 1054
ScaI AGTACT 1 cut(s) 1104
SchI GAGTC 2 cut(s) 194, 423
ScrFI CCNGG 2 cut(s) 165, 282
SduI GDGCHC 2 cut(s) 748, 1000
SfaNI GCATC 1 cut(s) 639
SfuI TTCGAA 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 233, 339
SmoI CTYRAG 2 cut(s) 233, 339
SnaBI TACGTA 1 cut(s) 1132
SpeI ACTAGT 2 cut(s) 383, 689
Sse9I AATT 7 cut(s) 253, 274, 461, 532, 1025, 1116, 1139
SsiI CCGC 4 cut(s) 182, 377, 971, 1052
SspMI CTAG 4 cut(s) 384, 690, 723, 1001
StyD4I CCNGG 2 cut(s) 163, 280
TaaI ACNGT 3 cut(s) 572, 768, 804
TaiI ACGT 3 cut(s) 654, 1040, 1134
TaqI TCGA 3 cut(s) 77, 203, 909
TasI AATT 7 cut(s) 253, 274, 461, 532, 1025, 1116, 1139
TatI WGTACW 2 cut(s) 816, 1102
TfiI GAWTC 2 cut(s) 6, 351
Tru1I TTAA 3 cut(s) 234, 246, 963
Tru9I TTAA 3 cut(s) 234, 246, 963
TscAI CASTG 4 cut(s) 415, 670, 748, 809
TseFI GTSAC 2 cut(s) 197, 768
TseI GCWGC 3 cut(s) 82, 827, 883
Tsp45I GTSAC 2 cut(s) 197, 768
TspDTI ATGAA 2 cut(s) 322, 547
TspRI CASTG 4 cut(s) 415, 670, 748, 809
Vha464I CTTAAG 1 cut(s) 233
VneI GTGCAC 1 cut(s) 744
XapI RAATTY 1 cut(s) 274
XceI RCATGY 1 cut(s) 502
XmnI GAANNNNTTC 2 cut(s) 500, 836
XspI CTAG 4 cut(s) 384, 690, 723, 1001
ZraI GACGTC 1 cut(s) 652
ZrmI AGTACT 1 cut(s) 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.