MD15G1008900.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001158
Physical Location & Seq
Reverse (-)
476208 .. 478535
2328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1008900.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGCTCATTTCTTTTAGATCATTTATTTTTGTGTACCTGCCACTTGGTGATATTGTCCAGATGCATGCAGTTGCAGGAGGACTAGGGCTATTTAGTCCTAAACTGGCCAGGCTGCAGATGCAGTCATATGTAATTTTCGTGATTTTCTTCTGCAATATTATTAGTAAGAATAACGTCATTGAGGCAGCTGCAACAGTCAAGCTGCCGAAGAATGTGACTGTCCCGGCTGTTATTGCATTCGGAGATTCGATTGTTGATACCGGCAACAACAACGAAAACTTCAAGACATTTGCACGCTGCAATTTCCTTCCCTACGGAAAAGACCTCAAGGGAGGAATGCCAACCGGCAGATATTCCAATGGAAAGGTTCCCACAGACTTCATAGTGGAAGCATTCGGAATCAAGGAGCTGTTGCCGGCATATTTGGATCCAACCTTGCAGCCTAATGACCTCCTTACCGGTGTTGTCATAGCTGCCGGTGGTGCAGGTTATGATCCATTGACAGCAAAACTTGCGGCAGTCGCATCGCTATCTGACCAATTGAAACAGTTCAAGGAGTACATAGAAAAGCTGAAAGGAATTGTTGGAGAGGAGAAAACAAACTTCATCATACAAAACAGTCTGATTCTTGTGGTAGCCGGCAGCAACGATATTTCTAATACCTATTTTCTTAGCGGTGTTAGGGAATTGGAGTATGACGTTCCTTCTTACACAGACTTCATGCTCAAATATGCCTCTGATTTTATCAAGGACTTGTATGGATTGGGGGCACGAAGAATAGGTGTACTAAGTGCACCACCTATCGGATGTGTGCCTTCTCAACGAACTGTAAAAGGAGGCATAAGAAGAGATTGTGACGAAAAACAAAACCAAGCATCACAACTGTTCAATTCCAAGCTCTCTGTGGAGATGGACCACCTTAACAAAAACCTGCCAAACTCGAGAGTGGTCTACATTGACATCTACAATTCGCTACTCGATCTCATCATAAACCCCGCTAAATATGGGTTTGAAGTTGCGAATAAAGGGTGTTGTGGTACTGGCACCATTGAAGTGACAAAACTGTGCAACCAGCTCCAACCTGCAGGAGTCTGCATAGATGACTCTAAGTATGTTTTCTGGGACAGCTACCATCCAACCGAAAGAGCGTACAAGATCATTGTGAAGCAGATTCTAGACAATTATACCAGCCACTTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

401

Amino Acids

44.12

Weight (kDa)

7.49

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 77 - 391 5e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 45, 476, 939, 1090
AccB1I GGYRCC 1 cut(s) 1043
AccI GTMKAC 1 cut(s) 951
AciI CCGC 3 cut(s) 515, 675, 996
AclWI GGATC 3 cut(s) 422, 435, 488
AcoI YGGCCR 1 cut(s) 105
AdeI CACNNNGTG 1 cut(s) 47
AfaI GTAC 5 cut(s) 35, 560, 786, 1039, 1151
AfiI CCNNNNNNNGG 1 cut(s) 803
AgeI ACCGGT 1 cut(s) 458
AgsI TTSAA 6 cut(s) 283, 544, 553, 889, 1013, 1052
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 8 cut(s) 188, 202, 409, 473, 571, 898, 1075, 1128
AluI AGCT 8 cut(s) 188, 202, 409, 473, 571, 898, 1075, 1128
Alw21I GWGCWC 1 cut(s) 796
Alw44I GTGCAC 1 cut(s) 792
AlwI GGATC 3 cut(s) 422, 435, 488
Ama87I CYCGRG 1 cut(s) 940
AoxI GGCC 1 cut(s) 105
ApaLI GTGCAC 1 cut(s) 792
ApeKI GCWGC 8 cut(s) 112, 185, 188, 202, 297, 439, 473, 642
AsiGI ACCGGT 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 548
AspS9I GGNCC 1 cut(s) 913
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 940
AvaII GGWCC 1 cut(s) 913
BaeGI GKGCMC 2 cut(s) 772, 796
BalI TGGCCA 1 cut(s) 107
BamHI GGATCC 1 cut(s) 427
BanI GGYRCC 1 cut(s) 1043
Bbv12I GWGCWC 1 cut(s) 796
BbvI GCAGC 8 cut(s) 99, 175, 189, 197, 284, 451, 460, 654
BccI CCATC 2 cut(s) 904, 1140
BcgI CGANNNNNNTGC 2 cut(s) 507, 541
BciT130I CCWGG 1 cut(s) 109
BcnI CCSGG 1 cut(s) 224
BfaI CTAG 2 cut(s) 83, 1175
BfmI CTRYAG 2 cut(s) 113, 1083
BfuAI ACCTGC 4 cut(s) 45, 476, 939, 1090
BisI GCNGC 9 cut(s) 113, 186, 189, 203, 298, 440, 474, 516, 643
BlsI GCNGC 9 cut(s) 114, 187, 190, 204, 299, 441, 475, 517, 644
Bme1390I CCNGG 2 cut(s) 109, 224
Bme18I GGWCC 1 cut(s) 913
BmeT110I CYCGRG 1 cut(s) 940
BmgT120I GGNCC 1 cut(s) 913
BmiI GGNNCC 3 cut(s) 369, 429, 1045
BmrFI CCNGG 2 cut(s) 109, 224
BmsI GCATC 4 cut(s) 51, 108, 533, 884
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 224
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 1 cut(s) 803
Bse118I RCCGGY 6 cut(s) 260, 344, 415, 458, 476, 638
Bse1I ACTGG 2 cut(s) 108, 1045
BseBI CCWGG 1 cut(s) 109
BseGI GGATG 2 cut(s) 812, 1132
BseLI CCNNNNNNNGG 1 cut(s) 803
BseNI ACTGG 2 cut(s) 108, 1045
BseRI GAGGAG 1 cut(s) 605
BseSI GKGCMC 2 cut(s) 772, 796
BseXI GCAGC 8 cut(s) 99, 175, 189, 197, 284, 451, 460, 654
BsgI GTGCAG 1 cut(s) 504
BshFI GGCC 1 cut(s) 107
BshNI GGYRCC 1 cut(s) 1043
BshTI ACCGGT 1 cut(s) 458
BsiHKAI GWGCWC 1 cut(s) 796
BsiHKCI CYCGRG 1 cut(s) 940
BsiSI CCGG 7 cut(s) 224, 261, 345, 416, 459, 477, 639
BslFI GGGAC 2 cut(s) 206, 1136
BslI CCNNNNNNNGG 1 cut(s) 803
BsmFI GGGAC 2 cut(s) 206, 1136
BsmI GAATGC 3 cut(s) 236, 342, 392
BsnI GGCC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 940
Bsp1286I GDGCHC 2 cut(s) 772, 796
Bsp143I GATC 5 cut(s) 17, 427, 493, 979, 1155
BspACI CCGC 3 cut(s) 515, 675, 996
BspANI GGCC 1 cut(s) 107
BspLI GGNNCC 3 cut(s) 369, 429, 1045
BspMAI CTGCAG 2 cut(s) 117, 1087
BspMI ACCTGC 4 cut(s) 45, 476, 939, 1090
BspPI GGATC 3 cut(s) 422, 435, 488
BspT107I GGYRCC 1 cut(s) 1043
BsrFI RCCGGY 6 cut(s) 260, 344, 415, 458, 476, 638
BsrI ACTGG 2 cut(s) 108, 1045
BssAI RCCGGY 6 cut(s) 260, 344, 415, 458, 476, 638
BssMI GATC 5 cut(s) 17, 427, 493, 979, 1155
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 7 cut(s) 196, 220, 549, 620, 829, 885, 1065
Bst6I CTCTTC 1 cut(s) 841
BstAPI GCANNNNNTGC 1 cut(s) 512
BstC8I GCNNGC 4 cut(s) 66, 295, 417, 640
BstDEI CTNAG 3 cut(s) 671, 788, 1107
BstF5I GGATG 2 cut(s) 812, 1132
BstKTI GATC 5 cut(s) 20, 430, 496, 982, 1158
BstMBI GATC 5 cut(s) 17, 427, 493, 979, 1155
BstMWI GCNNNNNNNGC 5 cut(s) 118, 233, 482, 512, 521
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 107, 222
BstSFI CTRYAG 2 cut(s) 113, 1083
BstSLI GKGCMC 2 cut(s) 772, 796
BstV1I GCAGC 8 cut(s) 99, 175, 189, 197, 284, 451, 460, 654
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 1 cut(s) 107
BtgZI GCGATG 1 cut(s) 510
BtsCI GGATG 2 cut(s) 812, 1132
BveI ACCTGC 4 cut(s) 45, 476, 939, 1090
Cac8I GCNNGC 4 cut(s) 66, 295, 417, 640
Cfr10I RCCGGY 6 cut(s) 260, 344, 415, 458, 476, 638
Cfr13I GGNCC 1 cut(s) 913
Csp6I GTAC 5 cut(s) 34, 559, 785, 1038, 1150
CspAI ACCGGT 1 cut(s) 458
CviAII CATG 2 cut(s) 65, 721
CviQI GTAC 5 cut(s) 34, 559, 785, 1038, 1150
DdeI CTNAG 3 cut(s) 671, 788, 1107
DpnI GATC 5 cut(s) 19, 429, 495, 981, 1157
DpnII GATC 5 cut(s) 17, 427, 493, 979, 1155
DraIII CACNNNGTG 1 cut(s) 47
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 841
EarI CTCTTC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 913
Eco88I CYCGRG 1 cut(s) 940
EcoRII CCWGG 1 cut(s) 107
EcoT22I ATGCAT 1 cut(s) 66
FaeI CATG 2 cut(s) 68, 724
FaqI GGGAC 2 cut(s) 206, 1136
FatI CATG 2 cut(s) 64, 720
FauI CCCGC 1 cut(s) 1003
FauNDI CATATG 1 cut(s) 127
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 9 cut(s) 113, 186, 189, 203, 298, 440, 474, 516, 643
FokI GGATG 2 cut(s) 819, 1119
Fsp4HI GCNGC 9 cut(s) 113, 186, 189, 203, 298, 440, 474, 516, 643
FspBI CTAG 2 cut(s) 83, 1175
GluI GCNGC 9 cut(s) 113, 186, 189, 203, 298, 440, 474, 516, 643
HaeIII GGCC 1 cut(s) 107
HapII CCGG 7 cut(s) 224, 261, 345, 416, 459, 477, 639
Hin1II CATG 2 cut(s) 68, 724
HinfI GANTC 6 cut(s) 245, 399, 625, 1089, 1103, 1171
HpaII CCGG 7 cut(s) 224, 261, 345, 416, 459, 477, 639
HphI GGTGA 1 cut(s) 59
Hpy166II GTNNAC 4 cut(s) 34, 785, 794, 952
Hpy188I TCNGA 7 cut(s) 242, 398, 535, 624, 739, 806, 1202
Hpy188III TCNNGA 5 cut(s) 58, 139, 283, 942, 1175
Hpy8I GTNNAC 4 cut(s) 34, 785, 794, 952
HpyAV CCTTC 3 cut(s) 317, 714, 825
HpyCH4III ACNGT 7 cut(s) 196, 220, 549, 620, 829, 885, 1065
HpyCH4IV ACGT 2 cut(s) 174, 699
HpyF10VI GCNNNNNNNGC 5 cut(s) 118, 233, 482, 512, 521
HpyF3I CTNAG 3 cut(s) 671, 788, 1107
HpySE526I ACGT 2 cut(s) 174, 699
Hsp92II CATG 2 cut(s) 68, 724
KroI GCCGGC 2 cut(s) 415, 638
KroNI GCCGGC 2 cut(s) 417, 640
Kzo9I GATC 5 cut(s) 17, 427, 493, 979, 1155
LmnI GCTCC 2 cut(s) 406, 1080
Lsp1109I GCAGC 8 cut(s) 99, 175, 189, 197, 284, 451, 460, 654
LweI GCATC 4 cut(s) 51, 108, 533, 884
MaeI CTAG 2 cut(s) 83, 1175
MaeII ACGT 2 cut(s) 174, 699
MaeIII GTNAC 3 cut(s) 214, 854, 1054
MalI GATC 5 cut(s) 19, 429, 495, 981, 1157
MboI GATC 5 cut(s) 17, 427, 493, 979, 1155
MboII GAAGA 4 cut(s) 139, 220, 786, 858
MfeI CAATTG 1 cut(s) 539
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 2 cut(s) 772, 796
MlsI TGGCCA 1 cut(s) 107
MluCI AATT 8 cut(s) 132, 301, 539, 579, 686, 889, 967, 1180
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 2 cut(s) 1097, 1098
MmeI TCCRAC 4 cut(s) 455, 565, 1102, 1160
MnlI CCTC 8 cut(s) 71, 175, 326, 335, 461, 583, 745, 830
Mox20I TGGCCA 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 66
MroNI GCCGGC 2 cut(s) 415, 638
MroXI GAANNNNTTC 1 cut(s) 548
MscI TGGCCA 1 cut(s) 107
MseI TTAA 1 cut(s) 921
MslI CAYNNNNRTG 1 cut(s) 1052
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 7 cut(s) 224, 261, 345, 416, 459, 477, 639
MspR9I CCNGG 2 cut(s) 109, 224
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 3 cut(s) 236, 342, 392
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 5 cut(s) 118, 233, 482, 512, 521
NaeI GCCGGC 2 cut(s) 417, 640
NciI CCSGG 1 cut(s) 224
NdeI CATATG 1 cut(s) 127
NdeII GATC 5 cut(s) 17, 427, 493, 979, 1155
NgoMIV GCCGGC 2 cut(s) 415, 638
NlaIII CATG 2 cut(s) 68, 724
NlaIV GGNNCC 3 cut(s) 369, 429, 1045
NmuCI GTSAC 3 cut(s) 214, 854, 1054
NsiI ATGCAT 1 cut(s) 66
NspI RCATGY 1 cut(s) 68
PaeI GCATGC 1 cut(s) 68
PaeR7I CTCGAG 1 cut(s) 940
PctI GAATGC 3 cut(s) 236, 342, 392
PdiI GCCGGC 2 cut(s) 417, 640
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 4 cut(s) 245, 399, 625, 1171
PinAI ACCGGT 1 cut(s) 458
PkrI GCNGC 9 cut(s) 114, 187, 190, 204, 299, 441, 475, 517, 644
PleI GAGTC 2 cut(s) 1097, 1097
PpsI GAGTC 2 cut(s) 1097, 1097
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 3 cut(s) 369, 429, 1045
PspPI GGNCC 1 cut(s) 913
PstI CTGCAG 2 cut(s) 117, 1087
PsuI RGATCY 1 cut(s) 427
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 5 cut(s) 35, 560, 786, 1039, 1151
RsaNI GTAC 5 cut(s) 34, 559, 785, 1038, 1150
RseI CAYNNNNRTG 1 cut(s) 1052
SaqAI TTAA 1 cut(s) 921
SatI GCNGC 9 cut(s) 113, 186, 189, 203, 298, 440, 474, 516, 643
Sau3AI GATC 5 cut(s) 17, 427, 493, 979, 1155
Sau96I GGNCC 1 cut(s) 913
SbfI CCTGCAGG 1 cut(s) 1087
SchI GAGTC 2 cut(s) 1097, 1098
ScrFI CCNGG 2 cut(s) 109, 224
SdaI CCTGCAGG 1 cut(s) 1087
SduI GDGCHC 2 cut(s) 772, 796
SfaNI GCATC 4 cut(s) 51, 108, 533, 884
SfcI CTRYAG 2 cut(s) 113, 1083
Sfr274I CTCGAG 1 cut(s) 940
SinI GGWCC 1 cut(s) 913
SlaI CTCGAG 1 cut(s) 940
SmiMI CAYNNNNRTG 1 cut(s) 1052
SmlI CTYRAG 2 cut(s) 326, 940
SmoI CTYRAG 2 cut(s) 326, 940
SphI GCATGC 1 cut(s) 68
Sse8387I CCTGCAGG 1 cut(s) 1087
Sse9I AATT 8 cut(s) 132, 301, 539, 579, 686, 889, 967, 1180
SsiI CCGC 3 cut(s) 515, 675, 996
SspI AATATT 1 cut(s) 157
SspMI CTAG 2 cut(s) 83, 1175
StyD4I CCNGG 2 cut(s) 107, 222
TaaI ACNGT 7 cut(s) 196, 220, 549, 620, 829, 885, 1065
TaiI ACGT 2 cut(s) 177, 702
TaqI TCGA 3 cut(s) 248, 941, 978
TasI AATT 8 cut(s) 132, 301, 539, 579, 686, 889, 967, 1180
TatI WGTACW 2 cut(s) 558, 784
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 4 cut(s) 245, 399, 625, 1171
Tru1I TTAA 1 cut(s) 921
Tru9I TTAA 1 cut(s) 921
TseFI GTSAC 3 cut(s) 214, 854, 1054
TseI GCWGC 8 cut(s) 112, 185, 188, 202, 297, 439, 473, 642
Tsp45I GTSAC 3 cut(s) 214, 854, 1054
TspDTI ATGAA 4 cut(s) 370, 595, 709, 1186
TspGWI ACGGA 1 cut(s) 330
VneI GTGCAC 1 cut(s) 792
VpaK11BI GGWCC 1 cut(s) 913
XbaI TCTAGA 1 cut(s) 1174
XceI RCATGY 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 1 cut(s) 901
XhoI CTCGAG 1 cut(s) 940
XmiI GTMKAC 1 cut(s) 951
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 2 cut(s) 83, 1175
Zsp2I ATGCAT 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.