Prupe.1G361800_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001158
Physical Location & Seq
Reverse (-)
33294360 .. 33296561
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G361800.1

Sequence Viewer

Length: 1161 bp
ATGTGTGGACATAACATGTTCAGGCAGAGACAGTGGTCACATGTTCCAGTGACCACATCAAACGCTATATGCATCTTTCTGTCTTACATTAACATATTTTTCCTGCTTTTCTTGTGCATTGTAAGCAATACTACAGAGACAGCAGCTACGACAGTCAAGCTACCGGGGAATGTAAGTGTTCCGGCAGTGATTATATTCGGAGATTCGATTGTTGATACGGGCAACAACAACAACAATTTCAAAACACTTGCACGCTGCAATTTCCTTCCATATGGGAAAGACCTCAAGGGAGGAATGCCAACGGGAAGATTTTCCAATGGAAAGGTTCCCTCGGACCTCATAGTGGAAGCATTTGGAATCAAAGAGCTGTTGCCAGCATATTTGGATCCAACCCTGCAGCCAGAGGATCTCCTTACTGGTGTAGTAATAGCTGCTGGTGGTGCAGGCTATGACCCTTTGACAGCACAACTCGCGGGAGTTGCATCACTATCTGACCAACTGAAGCAGTTCCAAGAGTACGTAGAAAAGCTGAAGGGAATTGCTGGAGAAGAGAGAACAAACTTCATCATAGCAAACTGTTTGATTTTTGTGGTAGCGGGCAGCAATGATATTTCTAACACCTATTTTCTTAGCGGTGCTAGGAAATTGGAGTATGATGTTCCTTCTTACACTGACTTCATGCTCAACTATGCCTCTCAGTACGTCAAGGACTTATATGGCCTGGGGGCACGAAGAATAGGTGTACTCAATGCGCCACCCATCGGATGTGTGCCATCTCAACGAACGATTGGAGGAGGCGTGCTAAGAGAATGTGACGACAAACAAAACCAAGCATCGCAGCTCTTCAATTTCAAACTCTCTGCAGAGATGGATGGCCTTAACAAAATCCTGCCCAACTCAAGAGTGGTCTATATTGAGATCTACAATCCACTACTTGATCTCATCATAAACCCTACAAAATACGGCTTTGAAGTTGTGAATAAAGGGTGCTGTGGTAGTGGGATTATTGAAGTAACAAAACTGTGCAACCAGTTTCAGCCTGCAGGCACCTGTTCAGATGACTCTAAGTATGTTTTTTGGGACAGCTACCATCCAACAGAAAGTGCATACAAGATCATTGTGCAGCAGATGCTAGACAAATATGCCAACCGCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

42.5

Weight (kDa)

5.74

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1046
AccII CGCG 1 cut(s) 473
AciI CCGC 4 cut(s) 473, 596, 633, 1150
AclWI GGATC 3 cut(s) 380, 393, 414
AcuI CTGAAG 2 cut(s) 521, 551
AfaI GTAC 3 cut(s) 518, 701, 744
AfiI CCNNNNNNNGG 2 cut(s) 343, 761
AflIII ACRYGT 2 cut(s) 15, 40
AgsI TTSAA 5 cut(s) 241, 847, 853, 971, 1010
AjnI CCWGG 1 cut(s) 720
AjuI GAANNNNNNNTTGG 1 cut(s) 1139
AluBI AGCT 7 cut(s) 146, 160, 367, 431, 529, 841, 1086
AluI AGCT 7 cut(s) 146, 160, 367, 431, 529, 841, 1086
Alw26I GTCTC 2 cut(s) 22, 131
AlwI GGATC 3 cut(s) 380, 393, 414
AoxI GGCC 2 cut(s) 718, 874
ApeKI GCWGC 7 cut(s) 143, 255, 397, 431, 600, 838, 1123
Asp700I GAANNNNTTC 2 cut(s) 310, 506
AspLEI GCGC 1 cut(s) 754
AspS9I GGNCC 1 cut(s) 334
AsuC2I CCSGG 1 cut(s) 165
AvaII GGWCC 1 cut(s) 334
BaeGI GKGCMC 1 cut(s) 730
BamHI GGATCC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 1046
BbvI GCAGC 7 cut(s) 155, 242, 409, 418, 612, 850, 1135
BccI CCATC 5 cut(s) 767, 781, 862, 866, 1098
BceAI ACGGC 1 cut(s) 979
BciT130I CCWGG 1 cut(s) 722
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 22, 131
BfaI CTAG 2 cut(s) 639, 1133
BfmI CTRYAG 4 cut(s) 132, 395, 861, 1041
BglII AGATCT 1 cut(s) 918
BisI GCNGC 7 cut(s) 144, 256, 398, 432, 601, 839, 1124
BlsI GCNGC 7 cut(s) 145, 257, 399, 433, 602, 840, 1125
Bme1390I CCNGG 2 cut(s) 165, 722
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 3 cut(s) 327, 387, 1048
BmrFI CCNGG 2 cut(s) 165, 722
BmsI GCATC 4 cut(s) 81, 491, 842, 1119
BoxI GACNNNNGTC 1 cut(s) 34
BpmI CTGGAG 1 cut(s) 564
BpuEI CTTGAG 2 cut(s) 269, 883
BpuMI CCSGG 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 520
BsaJI CCNNGG 3 cut(s) 164, 330, 721
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 761
Bse1I ACTGG 3 cut(s) 47, 421, 1030
Bse3DI GCAATG 1 cut(s) 610
BseBI CCWGG 1 cut(s) 722
BseDI CCNNGG 3 cut(s) 164, 330, 721
BseGI GGATG 3 cut(s) 770, 877, 1090
BseLI CCNNNNNNNGG 2 cut(s) 343, 761
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 1 cut(s) 710
BseNI ACTGG 3 cut(s) 47, 421, 1030
BseRI GAGGAG 1 cut(s) 807
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 7 cut(s) 155, 242, 409, 418, 612, 850, 1135
BsgI GTGCAG 2 cut(s) 462, 1142
Bsh1236I CGCG 1 cut(s) 473
BshFI GGCC 2 cut(s) 720, 876
BshNI GGYRCC 1 cut(s) 1046
BsiSI CCGG 2 cut(s) 164, 182
BslFI GGGAC 1 cut(s) 1094
BslI CCNNNNNNNGG 2 cut(s) 343, 761
BsmAI GTCTC 2 cut(s) 22, 131
BsmFI GGGAC 1 cut(s) 1094
BsmI GAATGC 1 cut(s) 300
BsnI GGCC 2 cut(s) 720, 876
Bsp1286I GDGCHC 1 cut(s) 730
Bsp143I GATC 5 cut(s) 385, 406, 918, 937, 1113
BspACI CCGC 4 cut(s) 473, 596, 633, 1150
BspANI GGCC 2 cut(s) 720, 876
BspCNI CTCAG 1 cut(s) 709
BspFNI CGCG 1 cut(s) 473
BspLI GGNNCC 3 cut(s) 327, 387, 1048
BspMAI CTGCAG 3 cut(s) 399, 865, 1045
BspPI GGATC 3 cut(s) 380, 393, 414
BspQI GCTCTTC 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 1046
BsrDI GCAATG 1 cut(s) 610
BsrI ACTGG 3 cut(s) 47, 421, 1030
BssECI CCNNGG 3 cut(s) 164, 330, 721
BssMI GATC 5 cut(s) 385, 406, 918, 937, 1113
Bst2UI CCWGG 1 cut(s) 722
Bst4CI ACNGT 4 cut(s) 33, 154, 578, 1023
Bst6I CTCTTC 2 cut(s) 543, 848
BstAPI GCANNNNNTGC 1 cut(s) 1129
BstBAI YACGTR 1 cut(s) 520
BstC8I GCNNGC 7 cut(s) 253, 375, 445, 598, 800, 1041, 1045
BstDEI CTNAG 4 cut(s) 629, 696, 803, 1065
BstF5I GGATG 3 cut(s) 770, 877, 1090
BstFNI CGCG 1 cut(s) 473
BstHHI GCGC 1 cut(s) 754
BstKTI GATC 5 cut(s) 388, 409, 921, 940, 1116
BstMAI GTCTC 2 cut(s) 22, 131
BstMBI GATC 5 cut(s) 385, 406, 918, 937, 1113
BstMWI GCNNNNNNNGC 5 cut(s) 123, 440, 470, 479, 1129
BstNI CCWGG 1 cut(s) 722
BstNSI RCATGY 2 cut(s) 19, 44
BstPAI GACNNNNGTC 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 163, 720
BstSFI CTRYAG 4 cut(s) 132, 395, 861, 1041
BstSLI GKGCMC 1 cut(s) 730
BstSNI TACGTA 1 cut(s) 520
BstUI CGCG 1 cut(s) 473
BstV1I GCAGC 7 cut(s) 155, 242, 409, 418, 612, 850, 1135
BstX2I RGATCY 3 cut(s) 385, 406, 918
BstYI RGATCY 3 cut(s) 385, 406, 918
BsuRI GGCC 2 cut(s) 720, 876
BtgZI GCGATG 1 cut(s) 819
BtsCI GGATG 3 cut(s) 770, 877, 1090
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 4 cut(s) 38, 54, 192, 669
Cac8I GCNNGC 7 cut(s) 253, 375, 445, 598, 800, 1041, 1045
CfoI GCGC 1 cut(s) 754
Cfr13I GGNCC 1 cut(s) 334
Csp6I GTAC 3 cut(s) 517, 700, 743
CviAII CATG 3 cut(s) 16, 41, 679
CviQI GTAC 3 cut(s) 517, 700, 743
DdeI CTNAG 4 cut(s) 629, 696, 803, 1065
DpnI GATC 5 cut(s) 387, 408, 920, 939, 1115
DpnII GATC 5 cut(s) 385, 406, 918, 937, 1113
Eam1104I CTCTTC 2 cut(s) 543, 848
EarI CTCTTC 2 cut(s) 543, 848
Eco105I TACGTA 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 334
Eco57I CTGAAG 2 cut(s) 521, 551
EcoRII CCWGG 1 cut(s) 720
EcoT22I ATGCAT 1 cut(s) 74
FaeI CATG 3 cut(s) 19, 44, 682
FaqI GGGAC 1 cut(s) 1094
FatI CATG 3 cut(s) 15, 40, 678
FauI CCCGC 2 cut(s) 466, 589
FauNDI CATATG 1 cut(s) 271
Fnu4HI GCNGC 7 cut(s) 144, 256, 398, 432, 601, 839, 1124
FokI GGATG 3 cut(s) 777, 884, 1077
Fsp4HI GCNGC 7 cut(s) 144, 256, 398, 432, 601, 839, 1124
FspBI CTAG 2 cut(s) 639, 1133
GlaI GCGC 1 cut(s) 753
GluI GCNGC 7 cut(s) 144, 256, 398, 432, 601, 839, 1124
GsuI CTGGAG 1 cut(s) 564
HaeIII GGCC 2 cut(s) 720, 876
HapII CCGG 2 cut(s) 164, 182
HhaI GCGC 1 cut(s) 754
Hin1II CATG 3 cut(s) 19, 44, 682
Hin6I GCGC 1 cut(s) 752
HinP1I GCGC 1 cut(s) 752
HinfI GANTC 3 cut(s) 203, 357, 1061
HpaII CCGG 2 cut(s) 164, 182
Hpy166II GTNNAC 2 cut(s) 8, 743
Hpy188I TCNGA 6 cut(s) 200, 334, 493, 764, 1057, 1160
Hpy188III TCNNGA 1 cut(s) 900
Hpy8I GTNNAC 2 cut(s) 8, 743
HpyAV CCTTC 3 cut(s) 275, 526, 672
HpyCH4III ACNGT 4 cut(s) 33, 154, 578, 1023
HpyCH4IV ACGT 2 cut(s) 519, 702
HpyF10VI GCNNNNNNNGC 5 cut(s) 123, 440, 470, 479, 1129
HpyF3I CTNAG 4 cut(s) 629, 696, 803, 1065
HpySE526I ACGT 2 cut(s) 519, 702
Hsp92II CATG 3 cut(s) 19, 44, 682
HspAI GCGC 1 cut(s) 752
Kzo9I GATC 5 cut(s) 385, 406, 918, 937, 1113
LguI GCTCTTC 1 cut(s) 848
Lsp1109I GCAGC 7 cut(s) 155, 242, 409, 418, 612, 850, 1135
LweI GCATC 4 cut(s) 81, 491, 842, 1119
MaeI CTAG 2 cut(s) 639, 1133
MaeII ACGT 2 cut(s) 519, 702
MaeIII GTNAC 4 cut(s) 36, 49, 812, 1012
MalI GATC 5 cut(s) 387, 408, 920, 939, 1115
MboI GATC 5 cut(s) 385, 406, 918, 937, 1113
MboII GAAGA 5 cut(s) 318, 560, 744, 835, 1147
MflI RGATCY 3 cut(s) 385, 406, 918
MhlI GDGCHC 1 cut(s) 730
MluCI AATT 5 cut(s) 235, 259, 537, 644, 847
MlyI GAGTC 1 cut(s) 1055
MmeI TCCRAC 2 cut(s) 413, 1118
MnlI CCTC 8 cut(s) 284, 293, 340, 347, 397, 703, 785, 788
Mph1103I ATGCAT 1 cut(s) 74
MroXI GAANNNNTTC 2 cut(s) 310, 506
MseI TTAA 2 cut(s) 90, 879
MspI CCGG 2 cut(s) 164, 182
MspR9I CCNGG 2 cut(s) 165, 722
Mva1269I GAATGC 1 cut(s) 300
MvaI CCWGG 1 cut(s) 722
MvnI CGCG 1 cut(s) 473
MwoI GCNNNNNNNGC 5 cut(s) 123, 440, 470, 479, 1129
NciI CCSGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 271
NdeII GATC 5 cut(s) 385, 406, 918, 937, 1113
NlaIII CATG 3 cut(s) 19, 44, 682
NlaIV GGNNCC 3 cut(s) 327, 387, 1048
NmuCI GTSAC 3 cut(s) 36, 49, 812
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 2 cut(s) 19, 44
PciI ACATGT 2 cut(s) 15, 40
PciSI GCTCTTC 1 cut(s) 848
PctI GAATGC 1 cut(s) 300
PdmI GAANNNNTTC 2 cut(s) 310, 506
PfeI GAWTC 2 cut(s) 203, 357
PkrI GCNGC 7 cut(s) 145, 257, 399, 433, 602, 840, 1125
PleI GAGTC 1 cut(s) 1055
PpsI GAGTC 1 cut(s) 1055
Ppu21I YACGTR 1 cut(s) 520
PscI ACATGT 2 cut(s) 15, 40
PshAI GACNNNNGTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 720
PspGI CCWGG 1 cut(s) 720
PspN4I GGNNCC 3 cut(s) 327, 387, 1048
PspPI GGNCC 1 cut(s) 334
PstI CTGCAG 3 cut(s) 399, 865, 1045
PsuI RGATCY 3 cut(s) 385, 406, 918
RsaI GTAC 3 cut(s) 518, 701, 744
RsaNI GTAC 3 cut(s) 517, 700, 743
SapI GCTCTTC 1 cut(s) 848
SaqAI TTAA 2 cut(s) 90, 879
SatI GCNGC 7 cut(s) 144, 256, 398, 432, 601, 839, 1124
Sau3AI GATC 5 cut(s) 385, 406, 918, 937, 1113
Sau96I GGNCC 1 cut(s) 334
SbfI CCTGCAGG 1 cut(s) 1045
SchI GAGTC 1 cut(s) 1055
ScrFI CCNGG 2 cut(s) 165, 722
SdaI CCTGCAGG 1 cut(s) 1045
SduI GDGCHC 1 cut(s) 730
SfaNI GCATC 4 cut(s) 81, 491, 842, 1119
SfcI CTRYAG 4 cut(s) 132, 395, 861, 1041
SinI GGWCC 1 cut(s) 334
SmlI CTYRAG 2 cut(s) 284, 898
SmoI CTYRAG 2 cut(s) 284, 898
SnaBI TACGTA 1 cut(s) 520
Sse8387I CCTGCAGG 1 cut(s) 1045
Sse9I AATT 5 cut(s) 235, 259, 537, 644, 847
SsiI CCGC 4 cut(s) 473, 596, 633, 1150
SspMI CTAG 2 cut(s) 639, 1133
StyD4I CCNGG 2 cut(s) 163, 720
TaaI ACNGT 4 cut(s) 33, 154, 578, 1023
TaiI ACGT 2 cut(s) 522, 705
TaqI TCGA 1 cut(s) 206
TasI AATT 5 cut(s) 235, 259, 537, 644, 847
TatI WGTACW 1 cut(s) 742
TfiI GAWTC 2 cut(s) 203, 357
Tru1I TTAA 2 cut(s) 90, 879
Tru9I TTAA 2 cut(s) 90, 879
TscAI CASTG 4 cut(s) 38, 54, 192, 676
TseFI GTSAC 3 cut(s) 36, 49, 812
TseI GCWGC 7 cut(s) 143, 255, 397, 431, 600, 838, 1123
Tsp45I GTSAC 3 cut(s) 36, 49, 812
TspDTI ATGAA 2 cut(s) 553, 667
TspRI CASTG 4 cut(s) 38, 54, 192, 676
VpaK11BI GGWCC 1 cut(s) 334
XceI RCATGY 2 cut(s) 19, 44
XcmI CCANNNNNNNNNTGG 1 cut(s) 901
XmnI GAANNNNTTC 2 cut(s) 310, 506
XspI CTAG 2 cut(s) 639, 1133
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.