MD15G1009000.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001158
Physical Location & Seq
Reverse (-)
487048 .. 494775
7728 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1009000.v1.1.491

Sequence Viewer

Length: 1980 bp
ATGACCACATTATTATTGAGATTTTTTCTGTTTGTTTGTTGTGGTCTGGTGATTTTGGGAAACCAAGCGGTTGGAGGTGATGTCGTTAAGCTGCCGCCCAATGCAACGATTCCCGCTGTGTTTATGTTTGGCGATTCCATCGTCGATACAGGGAACAATAACGTCAACCTGAAGACGCCAGCTCGGTGTAATTTCCCTCCCTACGGCAGGGAGTTCGAAGGAGGAATATCTACTGGGAGATATTCCAATGGAAAGGTTCCCTCTGACCTCGTAGTGGAAAAGTTAGGCATTAAAGAGCTCCTGCCAGCCTATTTGGATCCAAACCTACATCCCAATGATCTGCTTACAGGTGTCAGCTTTGCCGTTGGTGCCACCGGATATGATCCTATAACAGCCCAAATTGTGGCAGTTACACCACTGTCTAATCAACTGCGGCAATTTGAAGAGTACATAAGAAAGCTCAAAGGAATTGTGGGAGAAGAGAGAGCAAACTTCATCCTAGCCAACAGTCTATGTTTTGTGGTAGCAAGTAGCAACGATATTGCTAATACCTACTTTGTTGCCGGTGTTAGGAAGTTAGATGCACCACCTATTGGGTGTCTGCCATCTCAAAGGACTTTAGGAGGAGGCGTGGAAAGAGATTGTGCAGACAAACCAAACCAAGCAGCCATGCTCTTTAACTCCAAGCTCTCTGCTGAGCTGGACGACCTCAAGAATAACCTACCTAACTCGATGCTAATTTACGTCGACATCTACAACCCACTTCTGGATATGATCATCAACCCTACTAATTACGATGAAGAAGTCTATATGGAACAACCAGAAGGGTTTGTTCTCCCTGGGAATGAAAAGAAGGTTTGTAAATTAGTAAAGTCATTATATGGATTGAAGCAAGCACCAAAACAATGGCATGAAAGGTTTGATTTTGTCATTTTATCATATGGATTTAGACATAACAGTGCTGATAAATGCATATACTCTAAATTCACAAATGATTATGGTGTTGTTATATGTTTATATGTTGACGACTTGCTAATTTTTGGTACAAACATGAAAGGTGTATCTGAAACTAAAGAGTATCTAAATTCAAAATTCAAGATGAAGGATTTGAATGAAGTAGATACTATCTTGGGAATTAAAGTAAAGAAGCATAGTGGGGGCTACGCTTTGTGTCAGTCACATTATATAGAAAAATTGCTTCTTAAGTTTAAGCATCTACAAATAAAAGAAGCAAATACCCCTTATGACCATAGTGAAACTTTGCTTAATAATTCTGGTAGGTCTGTTGCACAGCTTAAGTATGCTAGTGTAATCGGAAGTTTAATGTATGCCATGCATTGTACCAGGCCTGATATCGCATTTGCAGTAAGCAAACTTTCTAGATACACTAGTCATCCAGGTACTGTTCATTGGAAAGCAATAAATAGAATTTTTGGTTATCTTAAAAGAACTATTAACCTGAGTTTAACTTACTCTGAGTTTCCAGCAGTACTTGAAGGATATTCAGATGCAAGTTGGATAACAAGTGTTAATGATAATAAGTCTACATCAGGGTGGATTTTTACAATTGCCGGAGGAGCAATTTCTTGGGCTTCGAAGAAGCAAACATGTATAGCACATTCAACTATGGAATCTGAATTTATAGCATTAGCAGCAGCAGGTAAAGAAGCGGAATGGATTAGAAATTTGTTATTGGAAATAGAGTTGTGGCCACAACCAATGCCATCCATTTCTTTATACTGTGATAGTGAGGCTACTTTGTCTAGAGCATACAATAAGGGACATGGGAAGGAACCTCACCTATATGAGCATAAGAAGTGTGGGAAGCTGGAGGTGTCCATTCTGTGCAATCGATTCGAGCCAGAGACGTGTACGGATGACTCTCATTATCTATATTGGGACAGCTACCATCCGACTGAGGGCGCCTACAAAATCATCGTCGACGAACTCATAACAAAATATCTCCCCCGCTTGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

660

Amino Acids

73.57

Weight (kDa)

6.61

Isoelectric Point (pI)

29.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 187 1.7e-08 GDSL-like Lipase/Acylhydrolase
RVT_2 PF07727 264 - 414 4.8e-39 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1649
AccB1I GGYRCC 2 cut(s) 368, 1922
AccB7I CCANNNNNTGG 2 cut(s) 403, 593
AccI GTMKAC 3 cut(s) 747, 1544, 1941
AciI CCGC 6 cut(s) 68, 95, 114, 433, 1670, 1969
AclWI GGATC 3 cut(s) 311, 324, 377
AcoI YGGCCR 1 cut(s) 1709
AcsI RAATTY 6 cut(s) 983, 1084, 1091, 1428, 1637, 1684
AcuI CTGAAG 1 cut(s) 191
AcyI GRCGYC 2 cut(s) 176, 1923
AfaI GTAC 6 cut(s) 449, 1045, 1342, 1402, 1491, 1873
AfiI CCNNNNNNNGG 9 cut(s) 203, 207, 274, 403, 570, 593, 766, 1919, 1972
AflII CTTAAG 2 cut(s) 1202, 1295
AflIII ACRYGT 2 cut(s) 1607, 1868
AgsI TTSAA 7 cut(s) 443, 889, 1089, 1096, 1111, 1496, 1623
AhlI ACTAGT 1 cut(s) 1388
AjiI CACGTC 1 cut(s) 1869
AjnI CCWGG 3 cut(s) 838, 1343, 1396
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 1859
AlwI GGATC 3 cut(s) 311, 324, 377
AlwNI CAGNNNCTG 1 cut(s) 1403
AoxI GGCC 2 cut(s) 1346, 1709
ApeKI GCWGC 4 cut(s) 91, 665, 1652, 1655
ApoI RAATTY 6 cut(s) 983, 1084, 1091, 1428, 1637, 1684
ArsI GACNNNNNNTTYG 2 cut(s) 1923, 1955
AspLEI GCGC 1 cut(s) 1925
AsuHPI GGTGA 3 cut(s) 61, 89, 1790
AsuII TTCGAA 2 cut(s) 216, 1595
BaeI ACNNNNGTAYC 2 cut(s) 1375, 1408
BalI TGGCCA 1 cut(s) 1711
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 2 cut(s) 368, 1922
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 4 cut(s) 78, 677, 1664, 1667
BccI CCATC 4 cut(s) 146, 613, 1732, 1917
BceAI ACGGC 2 cut(s) 220, 347
BcgI CGANNNNNNTGC 4 cut(s) 196, 230, 1837, 1871
BciT130I CCWGG 3 cut(s) 840, 1345, 1398
BclI TGATCA 1 cut(s) 774
BcoDI GTCTC 1 cut(s) 1859
BcuI ACTAGT 1 cut(s) 1388
BfaI CTAG 5 cut(s) 500, 1305, 1380, 1389, 1764
BfoI RGCGCY 1 cut(s) 1926
BfrI CTTAAG 2 cut(s) 1202, 1295
BfuAI ACCTGC 1 cut(s) 1649
BisI GCNGC 6 cut(s) 92, 95, 434, 666, 1653, 1656
BlpI GCTNAGC 1 cut(s) 696
BlsI GCNGC 6 cut(s) 93, 96, 435, 667, 1654, 1657
BmcAI AGTACT 1 cut(s) 1491
Bme1390I CCNGG 3 cut(s) 840, 1345, 1398
BmgBI CACGTC 1 cut(s) 1869
BmiI GGNNCC 5 cut(s) 258, 318, 370, 1794, 1924
BmrFI CCNGG 3 cut(s) 840, 1345, 1398
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 4 cut(s) 571, 723, 1222, 1498
BmuI ACTGGG 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 1 cut(s) 1850
Bpu1102I GCTNAGC 1 cut(s) 696
Bpu14I TTCGAA 2 cut(s) 216, 1595
BpuEI CTTGAG 1 cut(s) 695
Bsa29I ATCGAT 1 cut(s) 1852
BsaHI GRCGYC 2 cut(s) 176, 1923
BsaJI CCNNGG 2 cut(s) 838, 839
BsaWI WCCGGW 1 cut(s) 374
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 615, 645
Bsc4I CCNNNNNNNGG 9 cut(s) 203, 207, 274, 403, 570, 593, 766, 1919, 1972
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 1 cut(s) 238
BseBI CCWGG 3 cut(s) 840, 1345, 1398
BseCI ATCGAT 1 cut(s) 1852
BseDI CCNNGG 2 cut(s) 838, 839
BseGI GGATG 6 cut(s) 328, 495, 1393, 1724, 1882, 1909
BseLI CCNNNNNNNGG 9 cut(s) 203, 207, 274, 403, 570, 593, 766, 1919, 1972
BseMII CTCAG 4 cut(s) 687, 1451, 1467, 1908
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 2 cut(s) 639, 1590
BseXI GCAGC 4 cut(s) 78, 677, 1664, 1667
BsgI GTGCAG 1 cut(s) 666
BshFI GGCC 2 cut(s) 1348, 1711
BshNI GGYRCC 2 cut(s) 368, 1922
BshVI ATCGAT 1 cut(s) 1852
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 3 cut(s) 375, 564, 1572
BslFI GGGAC 2 cut(s) 1794, 1913
BslI CCNNNNNNNGG 9 cut(s) 203, 207, 274, 403, 570, 593, 766, 1919, 1972
BsmAI GTCTC 1 cut(s) 1859
BsmBI CGTCTC 1 cut(s) 1859
BsmFI GGGAC 2 cut(s) 1794, 1913
BsnI GGCC 2 cut(s) 1348, 1711
Bsp119I TTCGAA 2 cut(s) 216, 1595
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 4 cut(s) 316, 337, 382, 774
Bsp1720I GCTNAGC 1 cut(s) 696
BspACI CCGC 6 cut(s) 68, 95, 114, 433, 1670, 1969
BspANI GGCC 2 cut(s) 1348, 1711
BspCNI CTCAG 4 cut(s) 688, 1452, 1468, 1909
BspDI ATCGAT 1 cut(s) 1852
BspLI GGNNCC 5 cut(s) 258, 318, 370, 1794, 1924
BspMI ACCTGC 1 cut(s) 1649
BspPI GGATC 3 cut(s) 311, 324, 377
BspT104I TTCGAA 2 cut(s) 216, 1595
BspT107I GGYRCC 2 cut(s) 368, 1922
BspTI CTTAAG 2 cut(s) 1202, 1295
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 1 cut(s) 238
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 2 cut(s) 838, 839
BssMI GATC 4 cut(s) 316, 337, 382, 774
BssNI GRCGYC 2 cut(s) 176, 1923
Bst2UI CCWGG 3 cut(s) 840, 1345, 1398
Bst4CI ACNGT 5 cut(s) 420, 509, 959, 1405, 1742
Bst6I CTCTTC 2 cut(s) 438, 474
BstACI GRCGYC 2 cut(s) 176, 1923
BstAFI CTTAAG 2 cut(s) 1202, 1295
BstBI TTCGAA 2 cut(s) 216, 1595
BstC8I GCNNGC 3 cut(s) 180, 306, 894
BstDEI CTNAG 4 cut(s) 696, 1460, 1476, 1917
BstENI CCTNNNNNAGG 1 cut(s) 205
BstF5I GGATG 6 cut(s) 328, 495, 1393, 1724, 1882, 1909
BstH2I RGCGCY 1 cut(s) 1926
BstHHI GCGC 1 cut(s) 1925
BstKTI GATC 4 cut(s) 319, 340, 385, 777
BstMAI GTCTC 1 cut(s) 1859
BstMBI GATC 4 cut(s) 316, 337, 382, 774
BstMWI GCNNNNNNNGC 3 cut(s) 368, 1577, 1652
BstNI CCWGG 3 cut(s) 840, 1345, 1398
BstNSI RCATGY 1 cut(s) 1611
BstSCI CCNGG 3 cut(s) 838, 1343, 1396
BstV1I GCAGC 4 cut(s) 78, 677, 1664, 1667
BstV2I GAAGAC 1 cut(s) 179
BstX2I RGATCY 1 cut(s) 316
BstXI CCANNNNNNTGG 2 cut(s) 71, 906
BstYI RGATCY 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 1852
BsuRI GGCC 2 cut(s) 1348, 1711
BsuTUI ATCGAT 1 cut(s) 1852
BtrI CACGTC 1 cut(s) 1869
BtsCI GGATG 6 cut(s) 328, 495, 1393, 1724, 1882, 1909
BtsIMutI CAGTG 2 cut(s) 416, 964
BveI ACCTGC 1 cut(s) 1649
Cac8I GCNNGC 3 cut(s) 180, 306, 894
CaiI CAGNNNCTG 1 cut(s) 1403
CfoI GCGC 1 cut(s) 1925
Cfr10I RCCGGY 1 cut(s) 563
ClaI ATCGAT 1 cut(s) 1852
CseI GACGC 1 cut(s) 184
Csp6I GTAC 6 cut(s) 448, 1044, 1341, 1401, 1490, 1872
CviAII CATG 6 cut(s) 670, 911, 1051, 1333, 1608, 1784
CviQI GTAC 6 cut(s) 448, 1044, 1341, 1401, 1490, 1872
DdeI CTNAG 4 cut(s) 696, 1460, 1476, 1917
DinI GGCGCC 1 cut(s) 1924
DpnI GATC 4 cut(s) 318, 339, 384, 776
DpnII GATC 4 cut(s) 316, 337, 382, 774
EaeI YGGCCR 1 cut(s) 1709
Eam1104I CTCTTC 2 cut(s) 438, 474
EarI CTCTTC 2 cut(s) 438, 474
Ecl136II GAGCTC 1 cut(s) 298
Eco147I AGGCCT 1 cut(s) 1348
Eco24I GRGCYC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 1354
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 298
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoRII CCWGG 3 cut(s) 838, 1343, 1396
EcoRV GATATC 1 cut(s) 1354
EcoT22I ATGCAT 2 cut(s) 974, 1338
EcoT38I GRGCYC 1 cut(s) 300
EgeI GGCGCC 1 cut(s) 1924
EheI GGCGCC 1 cut(s) 1924
Esp3I CGTCTC 1 cut(s) 1859
FaeI CATG 6 cut(s) 673, 914, 1054, 1336, 1611, 1787
FaqI GGGAC 2 cut(s) 1794, 1913
FatI CATG 6 cut(s) 669, 910, 1050, 1332, 1607, 1783
FauI CCCGC 2 cut(s) 121, 1976
FauNDI CATATG 1 cut(s) 940
FbaI TGATCA 1 cut(s) 774
FblI GTMKAC 3 cut(s) 747, 1544, 1941
Fnu4HI GCNGC 6 cut(s) 92, 95, 434, 666, 1653, 1656
FokI GGATG 6 cut(s) 315, 482, 1380, 1711, 1889, 1896
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 6 cut(s) 92, 95, 434, 666, 1653, 1656
FspBI CTAG 5 cut(s) 500, 1305, 1380, 1389, 1764
GlaI GCGC 1 cut(s) 1924
GluI GCNGC 6 cut(s) 92, 95, 434, 666, 1653, 1656
GsuI CTGGAG 1 cut(s) 1850
HaeII RGCGCY 1 cut(s) 1926
HaeIII GGCC 2 cut(s) 1348, 1711
HapII CCGG 3 cut(s) 375, 564, 1572
HgaI GACGC 1 cut(s) 184
HhaI GCGC 1 cut(s) 1925
Hin1I GRCGYC 2 cut(s) 176, 1923
Hin1II CATG 6 cut(s) 673, 914, 1054, 1336, 1611, 1787
Hin6I GCGC 1 cut(s) 1923
HinP1I GCGC 1 cut(s) 1923
HincII GTYRAC 4 cut(s) 166, 748, 1024, 1942
HindII GTYRAC 4 cut(s) 166, 748, 1024, 1942
HinfI GANTC 5 cut(s) 109, 134, 1631, 1854, 1880
HpaII CCGG 3 cut(s) 375, 564, 1572
HphI GGTGA 3 cut(s) 61, 89, 1790
Hpy166II GTNNAC 6 cut(s) 166, 748, 1024, 1545, 1872, 1942
Hpy188I TCNGA 7 cut(s) 265, 1066, 1316, 1477, 1507, 1636, 1914
Hpy188III TCNNGA 5 cut(s) 712, 767, 1096, 1380, 1764
Hpy8I GTNNAC 6 cut(s) 166, 748, 1024, 1545, 1872, 1942
Hpy99I CGWCG 4 cut(s) 146, 749, 1943, 1946
HpyAV CCTTC 6 cut(s) 212, 818, 847, 1096, 1490, 1783
HpyCH4III ACNGT 5 cut(s) 420, 509, 959, 1405, 1742
HpyCH4IV ACGT 3 cut(s) 162, 744, 1868
HpyCH4V TGCA 9 cut(s) 104, 584, 647, 972, 1289, 1336, 1364, 1511, 1848
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 1577, 1652
HpyF3I CTNAG 4 cut(s) 696, 1460, 1476, 1917
HpySE526I ACGT 3 cut(s) 162, 744, 1868
Hsp92I GRCGYC 2 cut(s) 176, 1923
Hsp92II CATG 6 cut(s) 673, 914, 1054, 1336, 1611, 1787
HspAI GCGC 1 cut(s) 1923
KasI GGCGCC 1 cut(s) 1922
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 4 cut(s) 316, 337, 382, 774
LmnI GCTCC 2 cut(s) 303, 1577
Lsp1109I GCAGC 4 cut(s) 78, 677, 1664, 1667
LweI GCATC 4 cut(s) 571, 723, 1222, 1498
MaeI CTAG 5 cut(s) 500, 1305, 1380, 1389, 1764
MaeII ACGT 3 cut(s) 162, 744, 1868
MaeIII GTNAC 2 cut(s) 409, 1176
MalI GATC 4 cut(s) 318, 339, 384, 776
MboI GATC 4 cut(s) 316, 337, 382, 774
MboII GAAGA 5 cut(s) 184, 455, 491, 812, 1609
MfeI CAATTG 1 cut(s) 1566
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 300
MlsI TGGCCA 1 cut(s) 1711
MluNI TGGCCA 1 cut(s) 1711
Mly113I GGCGCC 1 cut(s) 1923
MlyI GAGTC 1 cut(s) 1874
MmeI TCCRAC 3 cut(s) 52, 1496, 1937
Mox20I TGGCCA 1 cut(s) 1711
Mph1103I ATGCAT 2 cut(s) 974, 1338
MscI TGGCCA 1 cut(s) 1711
MslI CAYNNNNRTG 4 cut(s) 333, 957, 1552, 1803
Msp20I TGGCCA 1 cut(s) 1711
MspA1I CMGCKG 1 cut(s) 116
MspCI CTTAAG 2 cut(s) 1202, 1295
MspI CCGG 3 cut(s) 375, 564, 1572
MspR9I CCNGG 3 cut(s) 840, 1345, 1398
MunI CAATTG 1 cut(s) 1566
MvaI CCWGG 3 cut(s) 840, 1345, 1398
MwoI GCNNNNNNNGC 3 cut(s) 368, 1577, 1652
NarI GGCGCC 1 cut(s) 1923
NdeI CATATG 1 cut(s) 940
NdeII GATC 4 cut(s) 316, 337, 382, 774
NlaIII CATG 6 cut(s) 673, 914, 1054, 1336, 1611, 1787
NlaIV GGNNCC 5 cut(s) 258, 318, 370, 1794, 1924
NmuCI GTSAC 1 cut(s) 1176
NsiI ATGCAT 2 cut(s) 974, 1338
NspI RCATGY 1 cut(s) 1611
NspV TTCGAA 2 cut(s) 216, 1595
PasI CCCWGGG 1 cut(s) 839
PceI AGGCCT 1 cut(s) 1348
PciI ACATGT 1 cut(s) 1607
PfeI GAWTC 4 cut(s) 109, 134, 1631, 1854
PflMI CCANNNNNTGG 2 cut(s) 403, 593
PkrI GCNGC 6 cut(s) 93, 96, 435, 667, 1654, 1657
PleI GAGTC 1 cut(s) 1874
PluTI GGCGCC 1 cut(s) 1926
PpsI GAGTC 1 cut(s) 1874
PscI ACATGT 1 cut(s) 1607
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 3 cut(s) 838, 1343, 1396
PspGI CCWGG 3 cut(s) 838, 1343, 1396
PspN4I GGNNCC 5 cut(s) 258, 318, 370, 1794, 1924
PstNI CAGNNNCTG 1 cut(s) 1403
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 6 cut(s) 449, 1045, 1342, 1402, 1491, 1873
RsaNI GTAC 6 cut(s) 448, 1044, 1341, 1401, 1490, 1872
RseI CAYNNNNRTG 4 cut(s) 333, 957, 1552, 1803
SacI GAGCTC 1 cut(s) 300
SalI GTCGAC 2 cut(s) 746, 1940
SatI GCNGC 6 cut(s) 92, 95, 434, 666, 1653, 1656
Sau3AI GATC 4 cut(s) 316, 337, 382, 774
ScaI AGTACT 1 cut(s) 1491
SchI GAGTC 1 cut(s) 1874
ScrFI CCNGG 3 cut(s) 840, 1345, 1398
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 4 cut(s) 571, 723, 1222, 1498
SfoI GGCGCC 1 cut(s) 1924
SfuI TTCGAA 2 cut(s) 216, 1595
SgrDI CGTCGACG 1 cut(s) 1940
SmiMI CAYNNNNRTG 4 cut(s) 333, 957, 1552, 1803
SmlI CTYRAG 3 cut(s) 710, 1202, 1295
SmoI CTYRAG 3 cut(s) 710, 1202, 1295
SpeI ACTAGT 1 cut(s) 1388
SseBI AGGCCT 1 cut(s) 1348
SsiI CCGC 6 cut(s) 68, 95, 114, 433, 1670, 1969
SspDI GGCGCC 1 cut(s) 1922
SspMI CTAG 5 cut(s) 500, 1305, 1380, 1389, 1764
SstI GAGCTC 1 cut(s) 300
StuI AGGCCT 1 cut(s) 1348
StyD4I CCNGG 3 cut(s) 838, 1343, 1396
TaaI ACNGT 5 cut(s) 420, 509, 959, 1405, 1742
TaiI ACGT 3 cut(s) 165, 747, 1871
TaqI TCGA 8 cut(s) 144, 216, 731, 747, 1595, 1852, 1857, 1941
TatI WGTACW 2 cut(s) 447, 1489
TauI GCSGC 2 cut(s) 97, 436
TfiI GAWTC 4 cut(s) 109, 134, 1631, 1854
TscAI CASTG 2 cut(s) 423, 964
TseFI GTSAC 1 cut(s) 1176
TseI GCWGC 4 cut(s) 91, 665, 1652, 1655
Tsp45I GTSAC 1 cut(s) 1176
TspDTI ATGAA 8 cut(s) 484, 813, 861, 927, 1067, 1115, 1128, 1397
TspGWI ACGGA 1 cut(s) 1889
TspRI CASTG 2 cut(s) 423, 964
Van91I CCANNNNNTGG 2 cut(s) 403, 593
Vha464I CTTAAG 2 cut(s) 1202, 1295
XagI CCTNNNNNAGG 1 cut(s) 205
XapI RAATTY 6 cut(s) 983, 1084, 1091, 1428, 1637, 1684
XbaI TCTAGA 2 cut(s) 1379, 1763
XceI RCATGY 1 cut(s) 1611
XmiI GTMKAC 3 cut(s) 747, 1544, 1941
XspI CTAG 5 cut(s) 500, 1305, 1380, 1389, 1764
ZrmI AGTACT 1 cut(s) 1491
Zsp2I ATGCAT 2 cut(s) 974, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.