Rh6CG447500

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001158
Physical Location & Seq
Forward (+)
62572904 .. 62576119
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG447500.1

Sequence Viewer

Length: 1098 bp
ATGGATGCAGCACGAGCAATAGTACTGTGTATTCTTTGTTATATTCTGATGTGCAATGATAGCGTCGGAGAGGGTTTAACCCTGAAGCAGATGAAGATTAAAATACCAGCGAACGTAAGTGTTCCGGCAGTGATTGTGTTTGGAGATTCAATAGTTGATACGGGCAACAATAACAACAACTTCATAACATTTGCTCGCTGCAATTTCCTCCCTTATGGCATAGACTTCCAAGGTGGACTTGCAACCGGACGATTCTCAAATGGAAAGGTTCCTGCTGACCTTGTAGTGGAAGCATTTGGAATTAAGGAGCTGTTGCCACCCTATTTGGATCCATCCCTAGAGCCTAATGATCTCCTTACTGGTATTAACTTTGCTGCTGGAGGTGTAGGTTATGACCCTTTGACATCAGAACTCGCGGCAGTTGAATCACTGTCCGATCAGCTGGAACAATTCAAAGGGTACATAGAAAAGCTGAAAGGAATCGTCGGAGAAAAGAGAACAAACTTCATCATATCAAATAGTTTGATTTTAGTGGTAGCAGGAAGCAACGACATTTCGAATACCTATTTTGTTAGTGGTATCAGGAAATTGCATTTCGATGTTCCTTCTTACACCGACTTCTTGCTCAACCATGCTTCGCAATTCTTCAAGGAATTATATGGATTGGGTGCACGAAGAATAGGCGTTTTTGGTGTACCAGCTATTGGATGTGTGCCATCCCAAAGAACAATTGGAGGAGGCATCGGAAGACGTTGTGCCGAGAATCAAAACCAAGCAGCGGAGCTCTTCAACTCCAAACTATCTGCAGAGACGAATCATCTTCGACACACACTCCCCAACTCAAAGTTCGTTTACATAGACGTCTACAACCCATTACTTGATATCATCACAAACTCTAATAAATACGGGTTTGAAGTTGCAGACAAAGGGTGTTGTGGTACTGGTTTGGTGGAAGTAACAAAACTGTGCAACCAGTTTCAGCGAACCTGTACGGACACCTCCAAATATGTCTTTTGGGATAGTTACCATCCAACTGAAAGAGCATACAGAATCCTTCTGCAGCAACTCCTAACGCAATCTGCAAGCGGTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

39.98

Weight (kDa)

6.0

Isoelectric Point (pI)

19.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 354 2.5e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 864
AccB7I CCANNNNNTGG 1 cut(s) 704
AccI GTMKAC 1 cut(s) 864
AccII CGCG 1 cut(s) 416
AciI CCGC 3 cut(s) 416, 779, 1086
AclWI GGATC 2 cut(s) 323, 336
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 1 cut(s) 861
AfaI GTAC 5 cut(s) 24, 461, 696, 940, 991
AfiI CCNNNNNNNGG 3 cut(s) 286, 704, 778
AgsI TTSAA 6 cut(s) 150, 425, 454, 649, 790, 914
AjuI GAANNNNNNNTTGG 2 cut(s) 830, 862
AluBI AGCT 5 cut(s) 310, 442, 472, 701, 784
AluI AGCT 5 cut(s) 310, 442, 472, 701, 784
Alw21I GWGCWC 2 cut(s) 673, 786
Alw26I GTCTC 1 cut(s) 803
Alw44I GTGCAC 1 cut(s) 669
AlwI GGATC 2 cut(s) 323, 336
ApaLI GTGCAC 1 cut(s) 669
ApeKI GCWGC 5 cut(s) 8, 198, 374, 776, 1060
AsuII TTCGAA 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 673
BamHI GGATCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 786
BauI CACGAG 1 cut(s) 12
BbsI GAAGAC 1 cut(s) 754
Bbv12I GWGCWC 2 cut(s) 673, 786
BbvI GCAGC 5 cut(s) 20, 185, 361, 788, 1072
BccI CCATC 3 cut(s) 340, 724, 1035
BcoDI GTCTC 1 cut(s) 803
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 2 cut(s) 804, 1058
BisI GCNGC 6 cut(s) 9, 199, 375, 417, 777, 1061
BlsI GCNGC 6 cut(s) 10, 200, 376, 418, 778, 1062
BmcAI AGTACT 1 cut(s) 24
BmiI GGNNCC 2 cut(s) 270, 330
BmsI GCATC 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 754
BpmI CTGGAG 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 557
BsaHI GRCGYC 1 cut(s) 861
BsaJI CCNNGG 1 cut(s) 229
BsaWI WCCGGW 1 cut(s) 245
BsaXI ACNNNNNCTCC 2 cut(s) 816, 846
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 704, 778
Bse1I ACTGG 3 cut(s) 364, 946, 973
Bse3DI GCAATG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 5 cut(s) 10, 332, 713, 716, 1027
BseLI CCNNNNNNNGG 3 cut(s) 286, 704, 778
BseMI GCAATG 1 cut(s) 61
BseNI ACTGG 3 cut(s) 364, 946, 973
BseRI GAGGAG 1 cut(s) 750
BseSI GKGCMC 1 cut(s) 673
BseXI GCAGC 5 cut(s) 20, 185, 361, 788, 1072
Bsh1236I CGCG 1 cut(s) 416
BsiHKAI GWGCWC 2 cut(s) 673, 786
BsiSI CCGG 2 cut(s) 125, 246
BslI CCNNNNNNNGG 3 cut(s) 286, 704, 778
BsmAI GTCTC 1 cut(s) 803
BsmBI CGTCTC 1 cut(s) 803
Bsp119I TTCGAA 1 cut(s) 557
Bsp1286I GDGCHC 2 cut(s) 673, 786
Bsp143I GATC 3 cut(s) 328, 349, 436
BspACI CCGC 3 cut(s) 416, 779, 1086
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 2 cut(s) 270, 330
BspMAI CTGCAG 2 cut(s) 808, 1062
BspPI GGATC 2 cut(s) 323, 336
BspQI GCTCTTC 1 cut(s) 791
BspT104I TTCGAA 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 61
BsrI ACTGG 3 cut(s) 364, 946, 973
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 3 cut(s) 328, 349, 436
BssNI GRCGYC 1 cut(s) 861
BssSI CACGAG 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 229
Bst2BI CACGAG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 27, 432, 966
Bst6I CTCTTC 1 cut(s) 791
BstACI GRCGYC 1 cut(s) 861
BstBI TTCGAA 1 cut(s) 557
BstC8I GCNNGC 2 cut(s) 196, 1084
BstF5I GGATG 5 cut(s) 10, 332, 713, 716, 1027
BstFNI CGCG 1 cut(s) 416
BstKTI GATC 3 cut(s) 331, 352, 439
BstMAI GTCTC 1 cut(s) 803
BstMBI GATC 3 cut(s) 328, 349, 436
BstMWI GCNNNNNNNGC 2 cut(s) 14, 60
BstSFI CTRYAG 2 cut(s) 804, 1058
BstSLI GKGCMC 1 cut(s) 673
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 5 cut(s) 20, 185, 361, 788, 1072
BstV2I GAAGAC 1 cut(s) 754
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BtsCI GGATG 5 cut(s) 10, 332, 713, 716, 1027
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 135, 428
Cac8I GCNNGC 2 cut(s) 196, 1084
CseI GACGC 1 cut(s) 52
Csp6I GTAC 5 cut(s) 23, 460, 695, 939, 990
CspCI CAANNNNNGTGG 2 cut(s) 306, 341
CviAII CATG 1 cut(s) 632
CviJI RGCY 6 cut(s) 310, 343, 442, 472, 701, 784
CviKI_1 RGCY 6 cut(s) 310, 343, 442, 472, 701, 784
CviQI GTAC 5 cut(s) 23, 460, 695, 939, 990
DpnI GATC 3 cut(s) 330, 351, 438
DpnII GATC 3 cut(s) 328, 349, 436
Eam1104I CTCTTC 1 cut(s) 791
EarI CTCTTC 1 cut(s) 791
Ecl136II GAGCTC 1 cut(s) 784
Eco130I CCWWGG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 786
Eco32I GATATC 1 cut(s) 883
Eco53kI GAGCTC 1 cut(s) 784
Eco57I CTGAAG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 784
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 786
ErhI CCWWGG 1 cut(s) 229
Esp3I CGTCTC 1 cut(s) 803
FaeI CATG 1 cut(s) 635
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FatI CATG 1 cut(s) 631
FblI GTMKAC 1 cut(s) 864
Fnu4HI GCNGC 6 cut(s) 9, 199, 375, 417, 777, 1061
FokI GGATG 5 cut(s) 17, 319, 703, 720, 1014
FriOI GRGCYC 1 cut(s) 786
Fsp4HI GCNGC 6 cut(s) 9, 199, 375, 417, 777, 1061
FspBI CTAG 1 cut(s) 338
GluI GCNGC 6 cut(s) 9, 199, 375, 417, 777, 1061
GsuI CTGGAG 1 cut(s) 399
HapII CCGG 2 cut(s) 125, 246
HgaI GACGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 861
Hin1II CATG 1 cut(s) 635
HinfI GANTC 7 cut(s) 146, 252, 425, 480, 763, 814, 1050
HpaII CCGG 2 cut(s) 125, 246
Hpy166II GTNNAC 5 cut(s) 236, 671, 695, 853, 865
Hpy188I TCNGA 6 cut(s) 48, 68, 409, 436, 488, 746
Hpy188III TCNNGA 1 cut(s) 583
Hpy8I GTNNAC 5 cut(s) 236, 671, 695, 853, 865
Hpy99I CGWCG 2 cut(s) 68, 488
HpyAV CCTTC 2 cut(s) 615, 1064
HpyCH4III ACNGT 3 cut(s) 27, 432, 966
HpyCH4IV ACGT 3 cut(s) 114, 751, 861
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 60
HpySE526I ACGT 3 cut(s) 114, 751, 861
Hsp92I GRCGYC 1 cut(s) 861
Hsp92II CATG 1 cut(s) 635
Kzo9I GATC 3 cut(s) 328, 349, 436
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 2 cut(s) 307, 781
Lsp1109I GCAGC 5 cut(s) 20, 185, 361, 788, 1072
LweI GCATC 1 cut(s) 750
MaeI CTAG 1 cut(s) 338
MaeII ACGT 3 cut(s) 114, 751, 861
MaeIII GTNAC 2 cut(s) 955, 1022
MalI GATC 3 cut(s) 330, 351, 438
MboI GATC 3 cut(s) 328, 349, 436
MboII GAAGA 6 cut(s) 106, 637, 687, 759, 778, 812
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 673, 786
MluCI AATT 7 cut(s) 202, 300, 449, 587, 641, 653, 729
MmeI TCCRAC 3 cut(s) 46, 466, 1055
MnlI CCTC 6 cut(s) 64, 218, 374, 728, 731, 1009
MseI TTAA 4 cut(s) 77, 99, 303, 366
MslI CAYNNNNRTG 1 cut(s) 597
MspA1I CMGCKG 2 cut(s) 442, 779
MspI CCGG 2 cut(s) 125, 246
MunI CAATTG 1 cut(s) 729
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 2 cut(s) 14, 60
NdeII GATC 3 cut(s) 328, 349, 436
NlaIII CATG 1 cut(s) 635
NlaIV GGNNCC 2 cut(s) 270, 330
NmeAIII GCCGAG 1 cut(s) 784
NspV TTCGAA 1 cut(s) 557
PciSI GCTCTTC 1 cut(s) 791
PfeI GAWTC 7 cut(s) 146, 252, 425, 480, 763, 814, 1050
PflMI CCANNNNNTGG 1 cut(s) 704
PkrI GCNGC 6 cut(s) 10, 200, 376, 418, 778, 1062
Psp124BI GAGCTC 1 cut(s) 786
PspN4I GGNNCC 2 cut(s) 270, 330
PstI CTGCAG 2 cut(s) 808, 1062
PsuI RGATCY 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 442
RsaI GTAC 5 cut(s) 24, 461, 696, 940, 991
RsaNI GTAC 5 cut(s) 23, 460, 695, 939, 990
RseI CAYNNNNRTG 1 cut(s) 597
SacI GAGCTC 1 cut(s) 786
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 4 cut(s) 77, 99, 303, 366
SatI GCNGC 6 cut(s) 9, 199, 375, 417, 777, 1061
Sau3AI GATC 3 cut(s) 328, 349, 436
ScaI AGTACT 1 cut(s) 24
SduI GDGCHC 2 cut(s) 673, 786
SfaNI GCATC 1 cut(s) 750
SfcI CTRYAG 2 cut(s) 804, 1058
SfuI TTCGAA 1 cut(s) 557
SmiMI CAYNNNNRTG 1 cut(s) 597
Sse9I AATT 7 cut(s) 202, 300, 449, 587, 641, 653, 729
SsiI CCGC 3 cut(s) 416, 779, 1086
SspMI CTAG 1 cut(s) 338
SstI GAGCTC 1 cut(s) 786
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 3 cut(s) 27, 432, 966
TaiI ACGT 3 cut(s) 117, 754, 864
TaqI TCGA 3 cut(s) 557, 597, 823
TasI AATT 7 cut(s) 202, 300, 449, 587, 641, 653, 729
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 7 cut(s) 146, 252, 425, 480, 763, 814, 1050
Tru1I TTAA 4 cut(s) 77, 99, 303, 366
Tru9I TTAA 4 cut(s) 77, 99, 303, 366
TscAI CASTG 2 cut(s) 135, 435
TseI GCWGC 5 cut(s) 8, 198, 374, 776, 1060
TspDTI ATGAA 3 cut(s) 107, 172, 496
TspGWI ACGGA 1 cut(s) 1007
TspRI CASTG 2 cut(s) 135, 435
Van91I CCANNNNNTGG 1 cut(s) 704
VneI GTGCAC 1 cut(s) 669
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmiI GTMKAC 1 cut(s) 864
XspI CTAG 1 cut(s) 338
ZraI GACGTC 1 cut(s) 862
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.