Rmu_sc0002586.1_g000041

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002586.1
OG0001158
Physical Location & Seq
Reverse (-)
163416 .. 167527
4112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002586.1_g000041.1.cds

Sequence Viewer

Length: 1170 bp
atggatccatccctagagcctaatgatctccttactggtgttagctttgctgctggaggtgtaggttatgaccctttgacatcagaagtcgcggcagttgaatcactgtccgatcagctggaacaattcaaagggtacatagaaaagctgaaaggaatcgtcggagaaaagagaacaaacttcatcatatcaaatagtttgatttttgtggtagcaggaagcaacgacatttcgagtacctattttgttagtggtatcaggaaattgcatttcgatgttccttcttacaccgacttcttgctcaaccatgcttcgcaattcttcaaggaattatacggattgggtgcacgaagaataggcatttttggtgtaccagctattgggtgtgtgccatcccaaagaacaattggaggaggcatcggaagacgttgtgccgagaatcaaaaccaagcagcggagctcttcaactccaaactatctgcagagatgaatcaccttagacacacactccccaactcaaagttcgtttacatagacgtctacaacccattacttgatatcatcacaaactcaaataaatacgggtttgaagttgcagacaaagggtgttgtggtactggtttggtggaagtaacaaaactgtgcaaccagtttcagcgaacctgtttcgctgctacgtgtggcttggtaatttggatttggaatggtggtggctcttctctttcttctcccctacttggcgatgacgacgacgattggcttcgacggtgtcccatgatgttaggtttggatcgggttcgtccttttggtggatcaaagcctctgatagaggttatcttggctgtgaaggcgctagacttctcgccttcaatctcatcggtgttaggcggagctgggccttttgctttgggcaatgtcggatggggacttgatgatggcaggactgctcaagtctatttgggttcaaattcgattgggtgcactttggcttctgtctctaagatcatcggcttggatttggtgagttgtcccgatggttggatcgatgggaccggcaaccatatgactggttttcagccttggtctgggggcgacgacgattcttggttgggatctaattttcttaatgtcaaggcaggcagcggtggtgcagtggcagtgctgagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

41.42

Weight (kDa)

5.09

Isoelectric Point (pI)

27.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 540
AccB7I CCANNNNNTGG 1 cut(s) 380
AccI GTMKAC 1 cut(s) 540
AccII CGCG 1 cut(s) 92
AciI CCGC 4 cut(s) 92, 455, 888, 1143
AclWI GGATC 5 cut(s) 12, 798, 820, 1049, 1120
AcsI RAATTY 1 cut(s) 967
AcyI GRCGYC 1 cut(s) 537
AfaI GTAC 4 cut(s) 137, 238, 372, 616
AfiI CCNNNNNNNGG 6 cut(s) 380, 454, 737, 809, 1038, 1085
AflIII ACRYGT 1 cut(s) 677
AgsI TTSAA 7 cut(s) 101, 130, 325, 466, 590, 870, 966
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 7 cut(s) 45, 118, 148, 377, 460, 893, 1167
AluI AGCT 7 cut(s) 45, 118, 148, 377, 460, 893, 1167
Alw21I GWGCWC 3 cut(s) 349, 462, 983
Alw26I GTCTC 1 cut(s) 1000
Alw44I GTGCAC 2 cut(s) 345, 979
AlwI GGATC 5 cut(s) 12, 798, 820, 1049, 1120
AoxI GGCC 1 cut(s) 896
ApaLI GTGCAC 2 cut(s) 345, 979
ApeKI GCWGC 4 cut(s) 50, 452, 671, 1140
ApoI RAATTY 1 cut(s) 967
AspLEI GCGC 1 cut(s) 853
AspS9I GGNCC 2 cut(s) 896, 1050
AsuHPI GGTGA 2 cut(s) 485, 1033
AvaII GGWCC 1 cut(s) 1050
BaeGI GKGCMC 2 cut(s) 349, 983
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 462
BbsI GAAGAC 1 cut(s) 430
Bbv12I GWGCWC 3 cut(s) 349, 462, 983
BbvI GCAGC 4 cut(s) 37, 464, 658, 1152
BccI CCATC 6 cut(s) 16, 400, 915, 929, 1028, 1040
BcoDI GTCTC 1 cut(s) 1000
BfaI CTAG 3 cut(s) 14, 854, 1168
BfmI CTRYAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 854
BisI GCNGC 5 cut(s) 51, 93, 453, 672, 1141
BlpI GCTNAGC 1 cut(s) 1163
BlsI GCNGC 5 cut(s) 52, 94, 454, 673, 1142
Bme18I GGWCC 1 cut(s) 1050
BmgT120I GGNCC 2 cut(s) 896, 1050
BmiI GGNNCC 2 cut(s) 6, 1051
BmsI GCATC 1 cut(s) 426
BpiI GAAGAC 1 cut(s) 430
BpmI CTGGAG 1 cut(s) 75
Bpu1102I GCTNAGC 1 cut(s) 1163
BpuEI CTTGAG 1 cut(s) 933
Bsa29I ATCGAT 1 cut(s) 1044
BsaAI YACGTR 1 cut(s) 678
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 1 cut(s) 1079
BsaXI ACNNNNNCTCC 2 cut(s) 492, 522
Bsc4I CCNNNNNNNGG 6 cut(s) 380, 454, 737, 809, 1038, 1085
Bse118I RCCGGY 1 cut(s) 1052
Bse1I ACTGG 4 cut(s) 40, 622, 649, 1072
Bse3DI GCAATG 1 cut(s) 919
BseCI ATCGAT 1 cut(s) 1044
BseDI CCNNGG 1 cut(s) 1079
BseGI GGATG 3 cut(s) 8, 392, 926
BseLI CCNNNNNNNGG 6 cut(s) 380, 454, 737, 809, 1038, 1085
BseMI GCAATG 1 cut(s) 919
BseMII CTCAG 1 cut(s) 1154
BseNI ACTGG 4 cut(s) 40, 622, 649, 1072
BseRI GAGGAG 1 cut(s) 426
BseSI GKGCMC 2 cut(s) 349, 983
BseXI GCAGC 4 cut(s) 37, 464, 658, 1152
BseYI CCCAGC 1 cut(s) 893
BsgI GTGCAG 1 cut(s) 1170
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 1 cut(s) 898
BshVI ATCGAT 1 cut(s) 1044
BsiHKAI GWGCWC 3 cut(s) 349, 462, 983
BsiSI CCGG 1 cut(s) 1053
BslFI GGGAC 4 cut(s) 756, 939, 1014, 1063
BslI CCNNNNNNNGG 6 cut(s) 380, 454, 737, 809, 1038, 1085
BsmAI GTCTC 1 cut(s) 1000
BsmFI GGGAC 4 cut(s) 756, 939, 1014, 1063
BsnI GGCC 1 cut(s) 898
Bsp1286I GDGCHC 3 cut(s) 349, 462, 983
Bsp143I GATC 8 cut(s) 4, 25, 112, 790, 812, 1002, 1041, 1112
Bsp1720I GCTNAGC 1 cut(s) 1163
BspACI CCGC 4 cut(s) 92, 455, 888, 1143
BspANI GGCC 1 cut(s) 898
BspCNI CTCAG 1 cut(s) 1155
BspDI ATCGAT 1 cut(s) 1044
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 2 cut(s) 6, 1051
BspMAI CTGCAG 1 cut(s) 484
BspPI GGATC 5 cut(s) 12, 798, 820, 1049, 1120
BspQI GCTCTTC 2 cut(s) 467, 721
BsrDI GCAATG 1 cut(s) 919
BsrFI RCCGGY 1 cut(s) 1052
BsrI ACTGG 4 cut(s) 40, 622, 649, 1072
BssAI RCCGGY 1 cut(s) 1052
BssECI CCNNGG 1 cut(s) 1079
BssMI GATC 8 cut(s) 4, 25, 112, 790, 812, 1002, 1041, 1112
BssNI GRCGYC 1 cut(s) 537
BssT1I CCWWGG 1 cut(s) 1079
Bst4CI ACNGT 3 cut(s) 108, 642, 768
Bst6I CTCTTC 2 cut(s) 467, 721
BstACI GRCGYC 1 cut(s) 537
BstBAI YACGTR 1 cut(s) 678
BstC8I GCNNGC 1 cut(s) 1138
BstDEI CTNAG 3 cut(s) 497, 999, 1163
BstF5I GGATG 3 cut(s) 8, 392, 926
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 1 cut(s) 853
BstKTI GATC 8 cut(s) 7, 28, 115, 793, 815, 1005, 1044, 1115
BstMAI GTCTC 1 cut(s) 1000
BstMBI GATC 8 cut(s) 4, 25, 112, 790, 812, 1002, 1041, 1112
BstMWI GCNNNNNNNGC 1 cut(s) 848
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 2 cut(s) 349, 983
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 4 cut(s) 37, 464, 658, 1152
BstV2I GAAGAC 1 cut(s) 430
BstX2I RGATCY 2 cut(s) 4, 1112
BstXI CCANNNNNNTGG 1 cut(s) 1067
BstYI RGATCY 2 cut(s) 4, 1112
Bsu15I ATCGAT 1 cut(s) 1044
BsuRI GGCC 1 cut(s) 898
BsuTUI ATCGAT 1 cut(s) 1044
BtgZI GCGATG 1 cut(s) 756
BtsCI GGATG 3 cut(s) 8, 392, 926
BtsI GCAGTG 2 cut(s) 1158, 1164
BtsIMutI CAGTG 3 cut(s) 104, 1158, 1164
Cac8I GCNNGC 1 cut(s) 1138
CfoI GCGC 1 cut(s) 853
Cfr10I RCCGGY 1 cut(s) 1052
Cfr13I GGNCC 2 cut(s) 896, 1050
ClaI ATCGAT 1 cut(s) 1044
Csp6I GTAC 4 cut(s) 136, 237, 371, 615
CviAII CATG 2 cut(s) 308, 775
CviQI GTAC 4 cut(s) 136, 237, 371, 615
DdeI CTNAG 3 cut(s) 497, 999, 1163
DpnI GATC 8 cut(s) 6, 27, 114, 792, 814, 1004, 1043, 1114
DpnII GATC 8 cut(s) 4, 25, 112, 790, 812, 1002, 1041, 1112
Eam1104I CTCTTC 2 cut(s) 467, 721
EarI CTCTTC 2 cut(s) 467, 721
EciI GGCGGA 1 cut(s) 903
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 1079
Eco24I GRGCYC 1 cut(s) 462
Eco32I GATATC 1 cut(s) 559
Eco47I GGWCC 1 cut(s) 1050
Eco53kI GAGCTC 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 460
EcoRV GATATC 1 cut(s) 559
EcoT14I CCWWGG 1 cut(s) 1079
EcoT38I GRGCYC 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 1079
FaeI CATG 2 cut(s) 311, 778
FaiI YATR 9 cut(s) 69, 140, 188, 309, 334, 533, 776, 1062, 1064
FaqI GGGAC 4 cut(s) 756, 939, 1014, 1063
FatI CATG 2 cut(s) 307, 774
FauNDI CATATG 1 cut(s) 1062
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 5 cut(s) 51, 93, 453, 672, 1141
FokI GGATG 2 cut(s) 379, 933
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 5 cut(s) 51, 93, 453, 672, 1141
FspBI CTAG 3 cut(s) 14, 854, 1168
GlaI GCGC 1 cut(s) 852
GluI GCNGC 5 cut(s) 51, 93, 453, 672, 1141
GsaI CCCAGC 1 cut(s) 897
GsuI CTGGAG 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 1 cut(s) 898
HapII CCGG 1 cut(s) 1053
HhaI GCGC 1 cut(s) 853
Hin1I GRCGYC 1 cut(s) 537
Hin1II CATG 2 cut(s) 311, 778
Hin6I GCGC 1 cut(s) 851
HinP1I GCGC 1 cut(s) 851
HinfI GANTC 5 cut(s) 101, 156, 439, 490, 1100
HpaII CCGG 1 cut(s) 1053
HphI GGTGA 2 cut(s) 485, 1033
Hpy166II GTNNAC 5 cut(s) 347, 371, 529, 541, 981
Hpy188I TCNGA 6 cut(s) 85, 112, 164, 422, 825, 920
Hpy188III TCNNGA 2 cut(s) 259, 1031
Hpy8I GTNNAC 5 cut(s) 347, 371, 529, 541, 981
Hpy99I CGWCG 6 cut(s) 164, 752, 755, 768, 1097, 1100
HpyAV CCTTC 3 cut(s) 291, 841, 876
HpyCH4III ACNGT 3 cut(s) 108, 642, 768
HpyCH4IV ACGT 3 cut(s) 427, 537, 677
HpyCH4V TGCA 7 cut(s) 268, 347, 482, 596, 645, 981, 1151
HpyF10VI GCNNNNNNNGC 1 cut(s) 848
HpyF3I CTNAG 3 cut(s) 497, 999, 1163
HpySE526I ACGT 3 cut(s) 427, 537, 677
Hsp92I GRCGYC 1 cut(s) 537
Hsp92II CATG 2 cut(s) 311, 778
HspAI GCGC 1 cut(s) 851
Kzo9I GATC 8 cut(s) 4, 25, 112, 790, 812, 1002, 1041, 1112
LguI GCTCTTC 2 cut(s) 467, 721
LmnI GCTCC 2 cut(s) 457, 890
Lsp1109I GCAGC 4 cut(s) 37, 464, 658, 1152
LweI GCATC 1 cut(s) 426
MaeI CTAG 3 cut(s) 14, 854, 1168
MaeII ACGT 3 cut(s) 427, 537, 677
MaeIII GTNAC 1 cut(s) 631
MalI GATC 8 cut(s) 6, 27, 114, 792, 814, 1004, 1043, 1114
MboI GATC 8 cut(s) 4, 25, 112, 790, 812, 1002, 1041, 1112
MboII GAAGA 6 cut(s) 313, 363, 435, 454, 708, 717
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 2 cut(s) 4, 1112
MhlI GDGCHC 3 cut(s) 349, 462, 983
MluCI AATT 8 cut(s) 125, 263, 317, 329, 405, 690, 967, 1117
MmeI TCCRAC 3 cut(s) 142, 898, 1019
MnlI CCTC 5 cut(s) 50, 404, 407, 823, 831
MseI TTAA 1 cut(s) 1125
MslI CAYNNNNRTG 1 cut(s) 273
MspA1I CMGCKG 3 cut(s) 118, 455, 1143
MspI CCGG 1 cut(s) 1053
MunI CAATTG 1 cut(s) 405
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 1 cut(s) 848
NdeI CATATG 1 cut(s) 1062
NdeII GATC 8 cut(s) 4, 25, 112, 790, 812, 1002, 1041, 1112
NlaIII CATG 2 cut(s) 311, 778
NlaIV GGNNCC 2 cut(s) 6, 1051
NmeAIII GCCGAG 1 cut(s) 460
PciSI GCTCTTC 2 cut(s) 467, 721
PfeI GAWTC 5 cut(s) 101, 156, 439, 490, 1100
PflFI GACNNNGTC 1 cut(s) 768
PflMI CCANNNNNTGG 1 cut(s) 380
PkrI GCNGC 5 cut(s) 52, 94, 454, 673, 1142
Ppu21I YACGTR 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 893
PspN4I GGNNCC 2 cut(s) 6, 1051
PspPI GGNCC 2 cut(s) 896, 1050
PstI CTGCAG 1 cut(s) 484
PsuI RGATCY 2 cut(s) 4, 1112
PsyI GACNNNGTC 1 cut(s) 768
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 4 cut(s) 137, 238, 372, 616
RsaNI GTAC 4 cut(s) 136, 237, 371, 615
RseI CAYNNNNRTG 1 cut(s) 273
SacI GAGCTC 1 cut(s) 462
SapI GCTCTTC 2 cut(s) 467, 721
SaqAI TTAA 1 cut(s) 1125
SatI GCNGC 5 cut(s) 51, 93, 453, 672, 1141
Sau3AI GATC 8 cut(s) 4, 25, 112, 790, 812, 1002, 1041, 1112
Sau96I GGNCC 2 cut(s) 896, 1050
SduI GDGCHC 3 cut(s) 349, 462, 983
SfaNI GCATC 1 cut(s) 426
SfcI CTRYAG 1 cut(s) 480
SinI GGWCC 1 cut(s) 1050
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 948
SmoI CTYRAG 1 cut(s) 948
Sse9I AATT 8 cut(s) 125, 263, 317, 329, 405, 690, 967, 1117
SsiI CCGC 4 cut(s) 92, 455, 888, 1143
SspMI CTAG 3 cut(s) 14, 854, 1168
SstI GAGCTC 1 cut(s) 462
StyI CCWWGG 1 cut(s) 1079
TaaI ACNGT 3 cut(s) 108, 642, 768
TaiI ACGT 3 cut(s) 430, 540, 680
TaqI TCGA 5 cut(s) 233, 273, 763, 971, 1044
TasI AATT 8 cut(s) 125, 263, 317, 329, 405, 690, 967, 1117
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 5 cut(s) 101, 156, 439, 490, 1100
Tru1I TTAA 1 cut(s) 1125
Tru9I TTAA 1 cut(s) 1125
TscAI CASTG 3 cut(s) 111, 1158, 1164
TseI GCWGC 4 cut(s) 50, 452, 671, 1140
TspDTI ATGAA 2 cut(s) 172, 503
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 3 cut(s) 111, 1158, 1164
Tth111I GACNNNGTC 1 cut(s) 768
Van91I CCANNNNNTGG 1 cut(s) 380
VneI GTGCAC 2 cut(s) 345, 979
VpaK11BI GGWCC 1 cut(s) 1050
XapI RAATTY 1 cut(s) 967
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 3 cut(s) 14, 854, 1168
ZraI GACGTC 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.