AT3G14820

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001158
Physical Location & Seq
Forward (+)
4978726 .. 4980241
1516 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G14820.1

Sequence Viewer

Length: 1056 bp
ATGGATCTGCATCTTATAGGTTTTCTCTTGTGGTTTTTTGTTGTACAAGTGACTACAAGCAGTGCTCATAGGAACATAACAACAACAATACCAGCACTAATAGTTTTTGGAGACTCAATCATGGATACAGGAAATAATAATGACATCCCCACTCTTCTTAAGTCCAATTTTCCTCCCTATGGTCGAGATTTTCCTGGTGCTATTCCTACCGGTAGATTTTCAGACGGGAAAGTTCCATCGGACATCATTGCGGAATCGTTAGGAATAGCAAAAACACTACCACCATATTTAGGCTCGAATCTAAAGCCACATGATCTTCTAAAAGGTGTAATCTTTGCTTCTGGAGGTTCCGGTTATGATCCATTAACATCTACATTACTGTCTGTAGTATCAATGTCAGACCAACTAAAGTATTTTCAAGAATACCTAGCAAAAATTAAGCAACACTTTGGAGAAGAAAAAGTTAAGTTCATATTGGAGAAAAGTGTGTTTCTTGTGGTCTCTAGCAGTAATGATCTCGCTGAGACTTATTGGGTTCGATCGGTCGAATATGATCGTAACTCATATGCTGAATATCTGGTTGAGTTGGCTTCAGAGTTCATCAAAGAATTATCTGAACTTGGAGCTAAAAACATTGGACTGTTTAGCGGAGTACCAGTAGGGTGTTTACCCGCGCAAAGAACACTTTTTGGAGGCTTTGAAAGAAAATGTTATGAAAAGTTGAATAACATGGCACTTCATTTCAATTCAAAGCTATCTTCTAGTTTGGATACTCTAAAGAAAGAACTGCCCAGCAGACTAATATTTATCGACGTTTACGATACTCTTCTCGATATAATTAAAAATCCTACAAATTACGGATTTAAAGTGGCAGATAAAGGATGTTGTGGTACAGGAAAAATTGAGTTGATGGAATTGTGCAATAAATTTACTCCTTTTACATGTTCGGATGCATCGACTCATGTGTTCTTCGACTCTTATCATCCGAGTGAAAAGGCATATCAGATTATCACTCATAAATTGTTAGCAAAATACCGTAAATACCTAAACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

39.19

Weight (kDa)

6.71

Isoelectric Point (pI)

26.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 340 1.2e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 674
AciI CCGC 3 cut(s) 251, 648, 672
AclWI GGATC 2 cut(s) 12, 353
AcsI RAATTY 1 cut(s) 926
AcuI CTGAAG 1 cut(s) 576
AfaI GTAC 3 cut(s) 45, 654, 892
AfiI CCNNNNNNNGG 2 cut(s) 179, 290
AflII CTTAAG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 941
AgeI ACCGGT 1 cut(s) 209
AgsI TTSAA 5 cut(s) 419, 701, 724, 745, 750
AjnI CCWGG 1 cut(s) 193
AluBI AGCT 2 cut(s) 626, 754
AluI AGCT 2 cut(s) 626, 754
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 3 cut(s) 105, 505, 518
AlwI GGATC 2 cut(s) 12, 353
ApoI RAATTY 1 cut(s) 926
AsiGI ACCGGT 1 cut(s) 209
AspLEI GCGC 1 cut(s) 676
Bbv12I GWGCWC 1 cut(s) 67
BccI CCATC 2 cut(s) 244, 904
BciT130I CCWGG 1 cut(s) 195
BciVI GTATCC 2 cut(s) 118, 763
BcoDI GTCTC 3 cut(s) 105, 505, 518
BfaI CTAG 3 cut(s) 428, 504, 762
BfmI CTRYAG 1 cut(s) 384
BfrI CTTAAG 1 cut(s) 158
BfuI GTATCC 2 cut(s) 118, 763
Bme1390I CCNGG 1 cut(s) 195
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 3 cut(s) 19, 940, 962
BpmI CTGGAG 1 cut(s) 363
BsaBI GATNNNNATC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 505
BsaWI WCCGGW 2 cut(s) 209, 350
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 290
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 656
Bse3DI GCAATG 1 cut(s) 246
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 195
BseGI GGATG 4 cut(s) 144, 887, 955, 982
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 2 cut(s) 179, 290
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 656
BseYI CCCAGC 1 cut(s) 791
Bsh1236I CGCG 1 cut(s) 674
Bsh1285I CGRYCG 2 cut(s) 542, 546
BshTI ACCGGT 1 cut(s) 209
BsiEI CGRYCG 2 cut(s) 542, 546
BsiHKAI GWGCWC 1 cut(s) 67
BsiSI CCGG 2 cut(s) 210, 351
BslI CCNNNNNNNGG 2 cut(s) 179, 290
BsmAI GTCTC 3 cut(s) 105, 505, 518
Bso31I GGTCTC 1 cut(s) 505
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 43
Bsp143I GATC 6 cut(s) 4, 313, 358, 514, 539, 553
BspACI CCGC 3 cut(s) 251, 648, 672
BspCNI CTCAG 1 cut(s) 514
BspFNI CGCG 1 cut(s) 674
BspLI GGNNCC 1 cut(s) 349
BspPI GGATC 2 cut(s) 12, 353
BspTI CTTAAG 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 505
BsrDI GCAATG 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 209
BsrGI TGTACA 1 cut(s) 43
BsrI ACTGG 1 cut(s) 656
BssAI RCCGGY 1 cut(s) 209
BssMI GATC 6 cut(s) 4, 313, 358, 514, 539, 553
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 3 cut(s) 381, 642, 1037
Bst6I CTCTTC 2 cut(s) 159, 831
BstAFI CTTAAG 1 cut(s) 158
BstAUI TGTACA 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 522
BstF5I GGATG 4 cut(s) 144, 887, 955, 982
BstFNI CGCG 1 cut(s) 674
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 6 cut(s) 7, 316, 361, 517, 542, 556
BstMAI GTCTC 3 cut(s) 105, 505, 518
BstMBI GATC 6 cut(s) 4, 313, 358, 514, 539, 553
BstMCI CGRYCG 2 cut(s) 542, 546
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 945
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 384
BstUI CGCG 1 cut(s) 674
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 2 cut(s) 118, 763
BtsCI GGATG 4 cut(s) 144, 887, 955, 982
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 1 cut(s) 67
CfoI GCGC 1 cut(s) 676
Cfr10I RCCGGY 1 cut(s) 209
Csp6I GTAC 3 cut(s) 44, 653, 891
CspAI ACCGGT 1 cut(s) 209
CviAII CATG 5 cut(s) 121, 311, 730, 942, 962
CviJI RGCY 6 cut(s) 294, 307, 590, 626, 696, 754
CviKI_1 RGCY 6 cut(s) 294, 307, 590, 626, 696, 754
CviQI GTAC 3 cut(s) 44, 653, 891
DdeI CTNAG 1 cut(s) 522
DpnI GATC 6 cut(s) 6, 315, 360, 516, 541, 555
DpnII GATC 6 cut(s) 4, 313, 358, 514, 539, 553
DraI TTTAAA 1 cut(s) 865
Eam1104I CTCTTC 2 cut(s) 159, 831
EarI CTCTTC 2 cut(s) 159, 831
Eco31I GGTCTC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 576
EcoRII CCWGG 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 955
FaeI CATG 5 cut(s) 124, 314, 733, 945, 965
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FatI CATG 5 cut(s) 120, 310, 729, 941, 961
FauI CCCGC 1 cut(s) 679
FauNDI CATATG 1 cut(s) 565
FokI GGATG 4 cut(s) 131, 894, 962, 969
FspBI CTAG 3 cut(s) 428, 504, 762
GlaI GCGC 1 cut(s) 675
GsaI CCCAGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 363
HapII CCGG 2 cut(s) 210, 351
HhaI GCGC 1 cut(s) 676
Hin1II CATG 5 cut(s) 124, 314, 733, 945, 965
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HinfI GANTC 5 cut(s) 113, 254, 298, 958, 974
HpaII CCGG 2 cut(s) 210, 351
Hpy166II GTNNAC 2 cut(s) 668, 817
Hpy188I TCNGA 8 cut(s) 223, 241, 400, 595, 616, 949, 987, 1005
Hpy188III TCNNGA 4 cut(s) 185, 342, 419, 830
Hpy8I GTNNAC 2 cut(s) 668, 817
Hpy99I CGWCG 1 cut(s) 815
HpyCH4III ACNGT 3 cut(s) 381, 642, 1037
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 3 cut(s) 10, 921, 953
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 5 cut(s) 124, 314, 733, 945, 965
HspAI GCGC 1 cut(s) 674
Kzo9I GATC 6 cut(s) 4, 313, 358, 514, 539, 553
LmnI GCTCC 1 cut(s) 623
LweI GCATC 3 cut(s) 19, 940, 962
MaeI CTAG 3 cut(s) 428, 504, 762
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 2 cut(s) 49, 557
MalI GATC 6 cut(s) 6, 315, 360, 516, 541, 555
MboI GATC 6 cut(s) 4, 313, 358, 514, 539, 553
MboII GAAGA 6 cut(s) 146, 308, 467, 750, 818, 961
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 67
MlyI GAGTC 3 cut(s) 107, 952, 968
MnlI CCTC 3 cut(s) 183, 338, 686
Mph1103I ATGCAT 1 cut(s) 955
MseI TTAA 6 cut(s) 159, 365, 438, 465, 840, 864
MslI CAYNNNNRTG 1 cut(s) 987
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 2 cut(s) 210, 351
MspR9I CCNGG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 195
MvnI CGCG 1 cut(s) 674
NdeI CATATG 1 cut(s) 565
NdeII GATC 6 cut(s) 4, 313, 358, 514, 539, 553
NlaIII CATG 5 cut(s) 124, 314, 733, 945, 965
NlaIV GGNNCC 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 49
NsiI ATGCAT 1 cut(s) 955
NspI RCATGY 1 cut(s) 945
PciI ACATGT 1 cut(s) 941
PcsI WCGNNNNNNNCGW 2 cut(s) 816, 953
PfeI GAWTC 2 cut(s) 254, 298
PinAI ACCGGT 1 cut(s) 209
Ple19I CGATCG 1 cut(s) 542
PleI GAGTC 3 cut(s) 107, 952, 968
PpsI GAGTC 3 cut(s) 107, 952, 968
PscI ACATGT 1 cut(s) 941
Psp6I CCWGG 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 791
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 349
PsuI RGATCY 1 cut(s) 4
PvuI CGATCG 1 cut(s) 542
RsaI GTAC 3 cut(s) 45, 654, 892
RsaNI GTAC 3 cut(s) 44, 653, 891
RseI CAYNNNNRTG 1 cut(s) 987
SaqAI TTAA 6 cut(s) 159, 365, 438, 465, 840, 864
Sau3AI GATC 6 cut(s) 4, 313, 358, 514, 539, 553
SchI GAGTC 3 cut(s) 107, 952, 968
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 1 cut(s) 67
SetI ASST 9 cut(s) 22, 328, 349, 429, 628, 756, 816, 1047, 1055
SfaNI GCATC 3 cut(s) 19, 940, 962
SfcI CTRYAG 1 cut(s) 384
SmiMI CAYNNNNRTG 1 cut(s) 987
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SsiI CCGC 3 cut(s) 251, 648, 672
SspI AATATT 1 cut(s) 804
SspMI CTAG 3 cut(s) 428, 504, 762
StyD4I CCNGG 1 cut(s) 193
TaaI ACNGT 3 cut(s) 381, 642, 1037
TaiI ACGT 1 cut(s) 816
TaqI TCGA 8 cut(s) 184, 296, 538, 546, 810, 831, 956, 972
TaqII GACCGA 1 cut(s) 532
TatI WGTACW 1 cut(s) 43
TfiI GAWTC 2 cut(s) 254, 298
Tru1I TTAA 6 cut(s) 159, 365, 438, 465, 840, 864
Tru9I TTAA 6 cut(s) 159, 365, 438, 465, 840, 864
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 49
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 4 cut(s) 460, 589, 728, 729
TspGWI ACGGA 1 cut(s) 873
TspRI CASTG 1 cut(s) 67
Vha464I CTTAAG 1 cut(s) 158
XapI RAATTY 1 cut(s) 926
XceI RCATGY 1 cut(s) 945
XspI CTAG 3 cut(s) 428, 504, 762
Zsp2I ATGCAT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.