Rh6DG434100

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001158
Physical Location & Seq
Forward (+)
61265777 .. 61268098
2322 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG434100.1

Sequence Viewer

Length: 1224 bp
ATGAGCTTAAAAACCAACATTTGTCACTTATTCTCTTGTATGCTTCCTCTTTGGATCGGAGGAAATTCCAAAAGAATGGGATTCCTCTTTATAGAAACCTTCTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTGGTTCCATAATGTCGTCTTCGAAGCAGCTCTTGATATTTGTTTGTAGTCTTGCTGTGGTTTTAGGGCAGTACCCCAGAATCACAGAGGGTCGGGTGAATATACCACCAGGAAAGTCAATGCCGGCGGTTATAGTGTTCGGTGATTCGATCATGGACACCGGCAACAATAACAATCTTAAGTCTGCTGTTAAATGCAATTTCCCTCCATATGGTGAAAATTTCCCAGGAAAAGTTCCAACCGGTAGATTTGGCAATGGAAAAGTCCCCTCAGACTTCATAGCTCAAGAACTGGGAATCAAAGAGTTTGTGCCGGCATATGCGGATACTAGTCTAAAACCTGAAGATCTCAGCACTGGAGTCTGCTTCGCTTCAGGTGGCACCGGATATGACCCTCAGACCCCCGAATTAGTAGCAGTTTTATCGCTATCGGAGCAGTTGAACATGTTCAAGGAGTATATAGGGAAGCTGAAAGCCAATTTTGGCGAGCAGAGAACAAACTTCATCCTCGCCAACAGTCTCTTTCTGGTGGTCGCCGGTAGCGATGACATTGCCAACACCTATTTCACTATTGGCATCAGAAAGGCAGAATATGACGTTCCTGCTTACACTGACCTTATGGTCAACTCTGCCACTAGTTTCCTTCAGGATTTGTATGCACTTGGGGCTAGAAGGTTCGGTGTTTTCAGTGCACCGCCGATAGGTTGTGTACCTTCACAAAGAACTCTAGGTGGAGGAATACAAAGACAGTGTGCAGAAGAGTACAACGACGCAGCGAAGCTATTCAACTCTAAGCTGTCGTCCAACATGCAGTCCTTGAATACAAAGCTGCCCGACAGTAGAATGGTTTATATCGATGTTTACACCCCTCTACTTGATGTCATTCTTCACCCTGCCAAATACGGGTTTAAAGTGGCGGAGAAAGGTTGCTGTGGCACAGGGGCACTAGAGGTAGCGATATTATGCAACAAATTGTCTCCAACGTGTGAAAATCCGGCGGATCATGTATTTTGGGATAGTTATCACCCTACTGAACAAGCATACAGAGTACTCATTCCTCCAGTTCTCCAAAAATACGTAAACCAATTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

407

Amino Acids

44.34

Weight (kDa)

6.88

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 87 - 394 8.4e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 509
AciI CCGC 5 cut(s) 257, 452, 824, 1046, 1127
AclWI GGATC 2 cut(s) 62, 1137
AcsI RAATTY 2 cut(s) 64, 349
AcuI CTGAAG 3 cut(s) 486, 492, 758
AfaI GTAC 4 cut(s) 203, 840, 893, 1179
AfiI CCNNNNNNNGG 3 cut(s) 341, 830, 1032
AflII CTTAAG 1 cut(s) 308
AflIII ACRYGT 2 cut(s) 573, 1112
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 4 cut(s) 571, 580, 916, 949
AhlI ACTAGT 2 cut(s) 458, 764
AjnI CCWGG 2 cut(s) 238, 355
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
AluBI AGCT 7 cut(s) 6, 160, 413, 598, 910, 925, 958
AluI AGCT 7 cut(s) 6, 160, 413, 598, 910, 925, 958
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 2 cut(s) 653, 1110
Alw44I GTGCAC 1 cut(s) 819
AlwI GGATC 2 cut(s) 62, 1137
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 3 cut(s) 157, 902, 958
ApoI RAATTY 2 cut(s) 64, 349
AsiGI ACCGGT 1 cut(s) 371
Asp700I GAANNNNTTC 2 cut(s) 575, 911
AsuHPI GGTGA 5 cut(s) 238, 284, 356, 1010, 1145
AsuII TTCGAA 1 cut(s) 152
BaeGI GKGCMC 2 cut(s) 823, 1075
BanI GGYRCC 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 3 cut(s) 169, 914, 945
BcgI CGANNNNNNTGC 4 cut(s) 534, 568, 662, 696
BciT130I CCWGG 2 cut(s) 240, 357
BciVI GTATCC 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 653, 1110
BcuI ACTAGT 2 cut(s) 458, 764
BfaI CTAG 5 cut(s) 459, 765, 798, 857, 1076
BfrI CTTAAG 1 cut(s) 308
BfuI GTATCC 1 cut(s) 448
BglII AGATCT 1 cut(s) 475
BisI GCNGC 3 cut(s) 158, 903, 959
BlsI GCNGC 3 cut(s) 159, 904, 960
BmcAI AGTACT 1 cut(s) 1179
Bme1390I CCNGG 2 cut(s) 240, 357
BmiI GGNNCC 2 cut(s) 136, 511
BmrFI CCNGG 2 cut(s) 240, 357
BmrI ACTGGG 1 cut(s) 431
BmsI GCATC 1 cut(s) 714
BmuI ACTGGG 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 2 cut(s) 507, 1173
Bpu14I TTCGAA 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 399
Bsa29I ATCGAT 1 cut(s) 984
BsaAI YACGTR 1 cut(s) 1207
BsaBI GATNNNNATC 1 cut(s) 1149
BsaJI CCNNGG 1 cut(s) 355
BsaWI WCCGGW 2 cut(s) 371, 512
Bsc4I CCNNNNNNNGG 3 cut(s) 341, 830, 1032
Bse118I RCCGGY 5 cut(s) 253, 290, 371, 442, 665
Bse1I ACTGG 3 cut(s) 426, 490, 1190
Bse3DI GCAATG 2 cut(s) 391, 678
Bse8I GATNNNNATC 1 cut(s) 1149
BseBI CCWGG 2 cut(s) 240, 357
BseCI ATCGAT 1 cut(s) 984
BseDI CCNNGG 1 cut(s) 355
BseGI GGATG 1 cut(s) 633
BseJI GATNNNNATC 1 cut(s) 1149
BseLI CCNNNNNNNGG 3 cut(s) 341, 830, 1032
BseMI GCAATG 2 cut(s) 391, 678
BseMII CTCAG 3 cut(s) 414, 493, 539
BseNI ACTGG 3 cut(s) 426, 490, 1190
BseSI GKGCMC 2 cut(s) 823, 1075
BseXI GCAGC 3 cut(s) 169, 914, 945
BsgI GTGCAG 1 cut(s) 903
BshNI GGYRCC 1 cut(s) 509
BshTI ACCGGT 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 984
BsiHKAI GWGCWC 1 cut(s) 823
BsiSI CCGG 7 cut(s) 254, 291, 372, 443, 513, 666, 1124
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 3 cut(s) 341, 830, 1032
BsmAI GTCTC 2 cut(s) 653, 1110
BsmFI GGGAC 1 cut(s) 380
Bsp119I TTCGAA 1 cut(s) 152
Bsp1286I GDGCHC 2 cut(s) 823, 1075
Bsp143I GATC 4 cut(s) 54, 279, 475, 1129
BspACI CCGC 5 cut(s) 257, 452, 824, 1046, 1127
BspCNI CTCAG 3 cut(s) 413, 492, 538
BspDI ATCGAT 1 cut(s) 984
BspLI GGNNCC 2 cut(s) 136, 511
BspPI GGATC 2 cut(s) 62, 1137
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 509
BspTI CTTAAG 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 391, 678
BsrFI RCCGGY 5 cut(s) 253, 290, 371, 442, 665
BsrI ACTGG 3 cut(s) 426, 490, 1190
BssAI RCCGGY 5 cut(s) 253, 290, 371, 442, 665
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 4 cut(s) 54, 279, 475, 1129
Bst2UI CCWGG 2 cut(s) 240, 357
Bst4CI ACNGT 3 cut(s) 647, 879, 968
Bst6I CTCTTC 2 cut(s) 109, 882
BstAFI CTTAAG 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 1207
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 3 cut(s) 255, 444, 617
BstDEI CTNAG 4 cut(s) 400, 479, 525, 921
BstF5I GGATG 1 cut(s) 633
BstKTI GATC 4 cut(s) 57, 282, 478, 1132
BstMAI GTCTC 2 cut(s) 653, 1110
BstMBI GATC 4 cut(s) 54, 279, 475, 1129
BstMWI GCNNNNNNNGC 2 cut(s) 562, 794
BstNI CCWGG 2 cut(s) 240, 357
BstNSI RCATGY 2 cut(s) 577, 940
BstSCI CCNGG 2 cut(s) 238, 355
BstSLI GKGCMC 2 cut(s) 823, 1075
BstSNI TACGTA 1 cut(s) 1207
BstV1I GCAGC 3 cut(s) 169, 914, 945
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 475
BstXI CCANNNNNNTGG 1 cut(s) 76
BstYI RGATCY 1 cut(s) 475
Bsu15I ATCGAT 1 cut(s) 984
BsuI GTATCC 1 cut(s) 448
BsuTUI ATCGAT 1 cut(s) 984
BtgZI GCGATG 1 cut(s) 687
BtsCI GGATG 1 cut(s) 633
BtsIMutI CAGTG 4 cut(s) 483, 738, 823, 884
Cac8I GCNNGC 3 cut(s) 255, 444, 617
Cfr10I RCCGGY 5 cut(s) 253, 290, 371, 442, 665
ClaI ATCGAT 1 cut(s) 984
CseI GACGC 1 cut(s) 908
Csp6I GTAC 4 cut(s) 202, 839, 892, 1178
CspAI ACCGGT 1 cut(s) 371
CviAII CATG 4 cut(s) 283, 574, 937, 1133
CviJI RGCY 9 cut(s) 6, 160, 413, 598, 605, 797, 910, 925, 958
CviKI_1 RGCY 9 cut(s) 6, 160, 413, 598, 605, 797, 910, 925, 958
CviQI GTAC 4 cut(s) 202, 839, 892, 1178
DdeI CTNAG 4 cut(s) 400, 479, 525, 921
DpnI GATC 4 cut(s) 56, 281, 477, 1131
DpnII GATC 4 cut(s) 54, 279, 475, 1129
DraI TTTAAA 1 cut(s) 1039
DrdI GACNNNNNNGTC 1 cut(s) 749
DseDI GACNNNNNNGTC 1 cut(s) 749
Eam1104I CTCTTC 2 cut(s) 109, 882
EarI CTCTTC 2 cut(s) 109, 882
EciI GGCGGA 2 cut(s) 1061, 1142
Eco105I TACGTA 1 cut(s) 1207
Eco57I CTGAAG 3 cut(s) 486, 492, 758
EcoRII CCWGG 2 cut(s) 238, 355
FaeI CATG 4 cut(s) 286, 577, 940, 1136
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 1 cut(s) 380
FatI CATG 4 cut(s) 282, 573, 936, 1132
FauNDI CATATG 2 cut(s) 340, 448
Fnu4HI GCNGC 3 cut(s) 158, 903, 959
FokI GGATG 1 cut(s) 620
Fsp4HI GCNGC 3 cut(s) 158, 903, 959
FspBI CTAG 5 cut(s) 459, 765, 798, 857, 1076
GluI GCNGC 3 cut(s) 158, 903, 959
GsuI CTGGAG 2 cut(s) 507, 1173
HapII CCGG 7 cut(s) 254, 291, 372, 443, 513, 666, 1124
HgaI GACGC 1 cut(s) 908
Hin1II CATG 4 cut(s) 286, 577, 940, 1136
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HinfI GANTC 5 cut(s) 81, 210, 275, 426, 489
HpaII CCGG 7 cut(s) 254, 291, 372, 443, 513, 666, 1124
HphI GGTGA 5 cut(s) 238, 284, 356, 1010, 1145
Hpy166II GTNNAC 5 cut(s) 754, 821, 839, 991, 1210
Hpy188I TCNGA 6 cut(s) 59, 403, 528, 562, 710, 1223
Hpy188III TCNNGA 3 cut(s) 163, 416, 776
Hpy8I GTNNAC 5 cut(s) 754, 821, 839, 991, 1210
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 4 cut(s) 109, 782, 795, 852
HpyCH4III ACNGT 3 cut(s) 647, 879, 968
HpyCH4IV ACGT 3 cut(s) 726, 1112, 1206
HpyCH4V TGCA 6 cut(s) 327, 788, 821, 884, 940, 1095
HpyF10VI GCNNNNNNNGC 2 cut(s) 562, 794
HpyF3I CTNAG 4 cut(s) 400, 479, 525, 921
HpySE526I ACGT 3 cut(s) 726, 1112, 1206
Hsp92II CATG 4 cut(s) 286, 577, 940, 1136
KroI GCCGGC 2 cut(s) 253, 442
KroNI GCCGGC 2 cut(s) 255, 444
Kzo9I GATC 4 cut(s) 54, 279, 475, 1129
LmnI GCTCC 1 cut(s) 562
Lsp1109I GCAGC 3 cut(s) 169, 914, 945
LweI GCATC 1 cut(s) 714
MaeI CTAG 5 cut(s) 459, 765, 798, 857, 1076
MaeII ACGT 3 cut(s) 726, 1112, 1206
MaeIII GTNAC 1 cut(s) 23
MalI GATC 4 cut(s) 56, 281, 477, 1131
MboI GATC 4 cut(s) 54, 279, 475, 1129
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 2 cut(s) 823, 1075
MluCI AATT 7 cut(s) 64, 328, 349, 536, 607, 1100, 1214
MlyI GAGTC 1 cut(s) 498
MmeI TCCRAC 3 cut(s) 392, 957, 1133
MroNI GCCGGC 2 cut(s) 253, 442
MroXI GAANNNNTTC 2 cut(s) 575, 911
MseI TTAA 4 cut(s) 8, 309, 321, 1038
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 7 cut(s) 254, 291, 372, 443, 513, 666, 1124
MspR9I CCNGG 2 cut(s) 240, 357
MvaI CCWGG 2 cut(s) 240, 357
MwoI GCNNNNNNNGC 2 cut(s) 562, 794
NaeI GCCGGC 2 cut(s) 255, 444
NdeI CATATG 2 cut(s) 340, 448
NdeII GATC 4 cut(s) 54, 279, 475, 1129
NgoMIV GCCGGC 2 cut(s) 253, 442
NlaIII CATG 4 cut(s) 286, 577, 940, 1136
NlaIV GGNNCC 2 cut(s) 136, 511
NmuCI GTSAC 1 cut(s) 23
NspI RCATGY 2 cut(s) 577, 940
NspV TTCGAA 1 cut(s) 152
PciI ACATGT 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PdiI GCCGGC 2 cut(s) 255, 444
PdmI GAANNNNTTC 2 cut(s) 575, 911
PfeI GAWTC 4 cut(s) 81, 210, 275, 426
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 3 cut(s) 159, 904, 960
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
Ppu21I YACGTR 1 cut(s) 1207
PscI ACATGT 1 cut(s) 573
Psp6I CCWGG 2 cut(s) 238, 355
PspGI CCWGG 2 cut(s) 238, 355
PspN4I GGNNCC 2 cut(s) 136, 511
PsuI RGATCY 1 cut(s) 475
RsaI GTAC 4 cut(s) 203, 840, 893, 1179
RsaNI GTAC 4 cut(s) 202, 839, 892, 1178
SaqAI TTAA 4 cut(s) 8, 309, 321, 1038
SatI GCNGC 3 cut(s) 158, 903, 959
Sau3AI GATC 4 cut(s) 54, 279, 475, 1129
ScaI AGTACT 1 cut(s) 1179
SchI GAGTC 1 cut(s) 498
ScrFI CCNGG 2 cut(s) 240, 357
SduI GDGCHC 2 cut(s) 823, 1075
SfaNI GCATC 1 cut(s) 714
SfuI TTCGAA 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 308, 414
SmoI CTYRAG 2 cut(s) 308, 414
SnaBI TACGTA 1 cut(s) 1207
SpeI ACTAGT 2 cut(s) 458, 764
Sse9I AATT 7 cut(s) 64, 328, 349, 536, 607, 1100, 1214
SsiI CCGC 5 cut(s) 257, 452, 824, 1046, 1127
SspMI CTAG 5 cut(s) 459, 765, 798, 857, 1076
StyD4I CCNGG 2 cut(s) 238, 355
TaaI ACNGT 3 cut(s) 647, 879, 968
TaiI ACGT 3 cut(s) 729, 1115, 1209
TaqI TCGA 3 cut(s) 152, 278, 984
TasI AATT 7 cut(s) 64, 328, 349, 536, 607, 1100, 1214
TatI WGTACW 2 cut(s) 891, 1177
TfiI GAWTC 4 cut(s) 81, 210, 275, 426
Tru1I TTAA 4 cut(s) 8, 309, 321, 1038
Tru9I TTAA 4 cut(s) 8, 309, 321, 1038
TscAI CASTG 4 cut(s) 490, 745, 823, 884
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 3 cut(s) 157, 902, 958
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 397, 622
TspRI CASTG 4 cut(s) 490, 745, 823, 884
Vha464I CTTAAG 1 cut(s) 308
VneI GTGCAC 1 cut(s) 819
XapI RAATTY 2 cut(s) 64, 349
XceI RCATGY 2 cut(s) 577, 940
XmnI GAANNNNTTC 2 cut(s) 575, 911
XspI CTAG 5 cut(s) 459, 765, 798, 857, 1076
ZrmI AGTACT 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.