Rh6CG447300

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001158
Physical Location & Seq
Forward (+)
62551074 .. 62553491
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG447300.1

Sequence Viewer

Length: 1215 bp
ATGAGCTTAAAAACCAACATTTGTCACTTATTCTCTTGTATGCTTCCTCTTTGGACCGGAGGAAATTCCAAAAGAATGGGATTCCTCTTTATAGAAACCTTCTCTTCTTCTTCTTCTTCCTCTGGTTCCATAATGTCGTCTTCGAAGCAGCTCTTGATATTTGTTTGTAGTCTTGCTGTGGTTTTAGGGCAGTACCCCAGAATCACGGAGGGTCGGGTGAATATACCACCAGGAAAGTCAGTGCCGGCGGTTATAGTGTTCGGTGATTCGATCATGGACACCGGCAACAATAACAATCTTAACTCTGCTGTTAAATGCAATTTCCCTCCATATGGTGAAAATTTCCCAGGAAAAGTTTCAACCGGTAGATTTGGCAATGGAAAAGTCCCCTCAGACTTCATAGCTCAAGAACTGGGAATCAAAGAGTTTGTGCCGGCATATGCGGATACTAGTCTAAAACCTGAAGATCTCAGCACTGGAGTCTGCTTCGCTTCAGGTGGCACCGGATATGACCCTCAGACCCCCGAATTAGTAGCAGTTATATCGCTATCGGAGCAGTTGAACATGTTCAAGGAGTATATAGGGAAGCTGAAAGCCAATTTTGGCGAGCAGAGAACAAGCTTCATCCTCGCCAACAGTCTCTTTCTGGTGGTCGCCGGTAGCGATGACATTGCCAACACCTATTTCACTATTGGCATCAGAAAGGCAGAATATGACGTCCCTGCTTACACTGACCTTATGGTCAACTCTGCCACTAGTTTCCTTCAGGATTTGTATGCACTTGGGGCTAGAAGGTTCGGTGTTTTCAGTGCACCACCGATAGGGTGTGTACCTTCACAAAGAACTCTAGGTGGAGGAATACAAAGACAGTGTGCAGAAGAGTACAACGACGCAGCGAAGCTATTCAACTCTAAGCTGTCGTCCAACATGCAGTCCTTGAATACAAAGCTGCCCGACAGTAGAATGGTTTATATCGATGTTTACACCCCTCTACTTGATGTCATTCTTCACCCTGCCAAATACGGGTTTAAAATGGTGGAGAAAGGTTGCTGTGGCACAGGGGCACTAGAGGTAGCGATATTATGCAACAAATTGTCTCCAACGTGTGAAAATCCGGCGGATCATGTATTTTGGGATAGTTATCACCCTACTGAACAAGCATACAGAGTACTCATTCCTCCAGTTCTCCAAAAATACGTAAACCAATTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

404

Amino Acids

44.04

Weight (kDa)

6.45

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 84 - 391 5.3e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 740
AatII GACGTC 1 cut(s) 720
AccB1I GGYRCC 1 cut(s) 500
AciI CCGC 3 cut(s) 248, 443, 1118
AclWI GGATC 1 cut(s) 1128
AcsI RAATTY 2 cut(s) 64, 340
AcuI CTGAAG 3 cut(s) 477, 483, 749
AcyI GRCGYC 1 cut(s) 717
AfaI GTAC 4 cut(s) 194, 831, 884, 1170
AfiI CCNNNNNNNGG 3 cut(s) 332, 821, 1023
AflIII ACRYGT 2 cut(s) 564, 1103
AgeI ACCGGT 1 cut(s) 362
AgsI TTSAA 5 cut(s) 360, 562, 571, 907, 940
AhlI ACTAGT 2 cut(s) 449, 755
AjnI CCWGG 2 cut(s) 229, 346
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 8 cut(s) 6, 151, 404, 589, 621, 901, 916, 949
AluI AGCT 8 cut(s) 6, 151, 404, 589, 621, 901, 916, 949
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 2 cut(s) 644, 1101
Alw44I GTGCAC 1 cut(s) 810
AlwI GGATC 1 cut(s) 1128
ApaLI GTGCAC 1 cut(s) 810
ApeKI GCWGC 3 cut(s) 148, 893, 949
ApoI RAATTY 2 cut(s) 64, 340
AsiGI ACCGGT 1 cut(s) 362
Asp700I GAANNNNTTC 3 cut(s) 355, 566, 902
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 5 cut(s) 229, 275, 347, 1001, 1136
AsuII TTCGAA 1 cut(s) 143
AvaII GGWCC 1 cut(s) 54
BaeGI GKGCMC 2 cut(s) 814, 1066
BanI GGYRCC 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 814
BbvI GCAGC 3 cut(s) 160, 905, 936
BcgI CGANNNNNNTGC 4 cut(s) 525, 559, 653, 687
BciT130I CCWGG 2 cut(s) 231, 348
BciVI GTATCC 1 cut(s) 439
BcoDI GTCTC 2 cut(s) 644, 1101
BcuI ACTAGT 2 cut(s) 449, 755
BfaI CTAG 5 cut(s) 450, 756, 789, 848, 1067
BfuI GTATCC 1 cut(s) 439
BglII AGATCT 1 cut(s) 466
BisI GCNGC 3 cut(s) 149, 894, 950
BlsI GCNGC 3 cut(s) 150, 895, 951
BmcAI AGTACT 1 cut(s) 1170
Bme1390I CCNGG 2 cut(s) 231, 348
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 2 cut(s) 127, 502
BmrFI CCNGG 2 cut(s) 231, 348
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 422
BpiI GAAGAC 1 cut(s) 132
BpmI CTGGAG 2 cut(s) 498, 1164
Bpu14I TTCGAA 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 390
Bsa29I ATCGAT 1 cut(s) 975
BsaAI YACGTR 1 cut(s) 1198
BsaBI GATNNNNATC 1 cut(s) 1140
BsaHI GRCGYC 1 cut(s) 717
BsaJI CCNNGG 1 cut(s) 346
BsaWI WCCGGW 3 cut(s) 56, 362, 503
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 821, 1023
Bse118I RCCGGY 5 cut(s) 244, 281, 362, 433, 656
Bse1I ACTGG 3 cut(s) 417, 481, 1181
Bse3DI GCAATG 2 cut(s) 382, 669
Bse8I GATNNNNATC 1 cut(s) 1140
BseBI CCWGG 2 cut(s) 231, 348
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 1 cut(s) 624
BseJI GATNNNNATC 1 cut(s) 1140
BseLI CCNNNNNNNGG 3 cut(s) 332, 821, 1023
BseMI GCAATG 2 cut(s) 382, 669
BseMII CTCAG 3 cut(s) 405, 484, 530
BseNI ACTGG 3 cut(s) 417, 481, 1181
BseSI GKGCMC 2 cut(s) 814, 1066
BseXI GCAGC 3 cut(s) 160, 905, 936
BsgI GTGCAG 1 cut(s) 894
BshNI GGYRCC 1 cut(s) 500
BshTI ACCGGT 1 cut(s) 362
BshVI ATCGAT 1 cut(s) 975
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 8 cut(s) 57, 245, 282, 363, 434, 504, 657, 1115
BslFI GGGAC 2 cut(s) 371, 704
BslI CCNNNNNNNGG 3 cut(s) 332, 821, 1023
BsmAI GTCTC 2 cut(s) 644, 1101
BsmFI GGGAC 2 cut(s) 371, 704
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 814, 1066
Bsp143I GATC 3 cut(s) 270, 466, 1120
BspACI CCGC 3 cut(s) 248, 443, 1118
BspCNI CTCAG 3 cut(s) 404, 483, 529
BspDI ATCGAT 1 cut(s) 975
BspLI GGNNCC 2 cut(s) 127, 502
BspPI GGATC 1 cut(s) 1128
BspT104I TTCGAA 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 500
BsrDI GCAATG 2 cut(s) 382, 669
BsrFI RCCGGY 5 cut(s) 244, 281, 362, 433, 656
BsrI ACTGG 3 cut(s) 417, 481, 1181
BssAI RCCGGY 5 cut(s) 244, 281, 362, 433, 656
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 3 cut(s) 270, 466, 1120
BssNI GRCGYC 1 cut(s) 717
Bst2UI CCWGG 2 cut(s) 231, 348
Bst4CI ACNGT 3 cut(s) 638, 870, 959
Bst6I CTCTTC 2 cut(s) 109, 873
BstACI GRCGYC 1 cut(s) 717
BstBAI YACGTR 1 cut(s) 1198
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 3 cut(s) 246, 435, 608
BstDEI CTNAG 4 cut(s) 391, 470, 516, 912
BstF5I GGATG 1 cut(s) 624
BstKTI GATC 3 cut(s) 273, 469, 1123
BstMAI GTCTC 2 cut(s) 644, 1101
BstMBI GATC 3 cut(s) 270, 466, 1120
BstMWI GCNNNNNNNGC 2 cut(s) 553, 785
BstNI CCWGG 2 cut(s) 231, 348
BstNSI RCATGY 2 cut(s) 568, 931
BstSCI CCNGG 2 cut(s) 229, 346
BstSLI GKGCMC 2 cut(s) 814, 1066
BstSNI TACGTA 1 cut(s) 1198
BstV1I GCAGC 3 cut(s) 160, 905, 936
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 76
BstYI RGATCY 1 cut(s) 466
Bsu15I ATCGAT 1 cut(s) 975
BsuI GTATCC 1 cut(s) 439
BsuTUI ATCGAT 1 cut(s) 975
BtgZI GCGATG 1 cut(s) 678
BtsCI GGATG 1 cut(s) 624
BtsIMutI CAGTG 5 cut(s) 246, 474, 729, 814, 875
Cac8I GCNNGC 3 cut(s) 246, 435, 608
Cfr10I RCCGGY 5 cut(s) 244, 281, 362, 433, 656
Cfr13I GGNCC 1 cut(s) 54
ClaI ATCGAT 1 cut(s) 975
CseI GACGC 1 cut(s) 899
Csp6I GTAC 4 cut(s) 193, 830, 883, 1169
CspAI ACCGGT 1 cut(s) 362
CviAII CATG 4 cut(s) 274, 565, 928, 1124
CviQI GTAC 4 cut(s) 193, 830, 883, 1169
DdeI CTNAG 4 cut(s) 391, 470, 516, 912
DpnI GATC 3 cut(s) 272, 468, 1122
DpnII GATC 3 cut(s) 270, 466, 1120
DraI TTTAAA 1 cut(s) 1030
DrdI GACNNNNNNGTC 1 cut(s) 740
DseDI GACNNNNNNGTC 1 cut(s) 740
Eam1104I CTCTTC 2 cut(s) 109, 873
EarI CTCTTC 2 cut(s) 109, 873
EciI GGCGGA 1 cut(s) 1133
Eco105I TACGTA 1 cut(s) 1198
Eco47I GGWCC 1 cut(s) 54
Eco57I CTGAAG 3 cut(s) 477, 483, 749
EcoRII CCWGG 2 cut(s) 229, 346
FaeI CATG 4 cut(s) 277, 568, 931, 1127
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 2 cut(s) 371, 704
FatI CATG 4 cut(s) 273, 564, 927, 1123
FauNDI CATATG 2 cut(s) 331, 439
Fnu4HI GCNGC 3 cut(s) 149, 894, 950
FokI GGATG 1 cut(s) 611
Fsp4HI GCNGC 3 cut(s) 149, 894, 950
FspBI CTAG 5 cut(s) 450, 756, 789, 848, 1067
GluI GCNGC 3 cut(s) 149, 894, 950
GsuI CTGGAG 2 cut(s) 498, 1164
HapII CCGG 8 cut(s) 57, 245, 282, 363, 434, 504, 657, 1115
HgaI GACGC 1 cut(s) 899
Hin1I GRCGYC 1 cut(s) 717
Hin1II CATG 4 cut(s) 277, 568, 931, 1127
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HindIII AAGCTT 1 cut(s) 619
HinfI GANTC 5 cut(s) 81, 201, 266, 417, 480
HpaII CCGG 8 cut(s) 57, 245, 282, 363, 434, 504, 657, 1115
HphI GGTGA 5 cut(s) 229, 275, 347, 1001, 1136
Hpy166II GTNNAC 5 cut(s) 745, 812, 830, 982, 1201
Hpy188I TCNGA 4 cut(s) 394, 519, 553, 701
Hpy188III TCNNGA 3 cut(s) 154, 407, 767
Hpy8I GTNNAC 5 cut(s) 745, 812, 830, 982, 1201
Hpy99I CGWCG 1 cut(s) 893
HpyAV CCTTC 4 cut(s) 109, 773, 786, 843
HpyCH4III ACNGT 3 cut(s) 638, 870, 959
HpyCH4IV ACGT 3 cut(s) 717, 1103, 1197
HpyCH4V TGCA 6 cut(s) 318, 779, 812, 875, 931, 1086
HpyF10VI GCNNNNNNNGC 2 cut(s) 553, 785
HpyF3I CTNAG 4 cut(s) 391, 470, 516, 912
HpySE526I ACGT 3 cut(s) 717, 1103, 1197
Hsp92I GRCGYC 1 cut(s) 717
Hsp92II CATG 4 cut(s) 277, 568, 931, 1127
KroI GCCGGC 2 cut(s) 244, 433
KroNI GCCGGC 2 cut(s) 246, 435
Kzo9I GATC 3 cut(s) 270, 466, 1120
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 3 cut(s) 160, 905, 936
LweI GCATC 1 cut(s) 705
MaeI CTAG 5 cut(s) 450, 756, 789, 848, 1067
MaeII ACGT 3 cut(s) 717, 1103, 1197
MaeIII GTNAC 1 cut(s) 23
MalI GATC 3 cut(s) 272, 468, 1122
MboI GATC 3 cut(s) 270, 466, 1120
MboII GAAGA 9 cut(s) 96, 99, 102, 105, 108, 132, 476, 890, 998
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 2 cut(s) 814, 1066
MluCI AATT 7 cut(s) 64, 319, 340, 527, 598, 1091, 1205
MlyI GAGTC 1 cut(s) 489
MmeI TCCRAC 2 cut(s) 948, 1124
MroNI GCCGGC 2 cut(s) 244, 433
MroXI GAANNNNTTC 3 cut(s) 355, 566, 902
MseI TTAA 4 cut(s) 8, 300, 312, 1029
MspI CCGG 8 cut(s) 57, 245, 282, 363, 434, 504, 657, 1115
MspR9I CCNGG 2 cut(s) 231, 348
MvaI CCWGG 2 cut(s) 231, 348
MwoI GCNNNNNNNGC 2 cut(s) 553, 785
NaeI GCCGGC 2 cut(s) 246, 435
NdeI CATATG 2 cut(s) 331, 439
NdeII GATC 3 cut(s) 270, 466, 1120
NgoMIV GCCGGC 2 cut(s) 244, 433
NlaIII CATG 4 cut(s) 277, 568, 931, 1127
NlaIV GGNNCC 2 cut(s) 127, 502
NmuCI GTSAC 1 cut(s) 23
NspI RCATGY 2 cut(s) 568, 931
NspV TTCGAA 1 cut(s) 143
PciI ACATGT 1 cut(s) 564
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PdiI GCCGGC 2 cut(s) 246, 435
PdmI GAANNNNTTC 3 cut(s) 355, 566, 902
PfeI GAWTC 4 cut(s) 81, 201, 266, 417
PinAI ACCGGT 1 cut(s) 362
PkrI GCNGC 3 cut(s) 150, 895, 951
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
Ppu21I YACGTR 1 cut(s) 1198
PscI ACATGT 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 229, 346
PspGI CCWGG 2 cut(s) 229, 346
PspN4I GGNNCC 2 cut(s) 127, 502
PspPI GGNCC 1 cut(s) 54
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 4 cut(s) 194, 831, 884, 1170
RsaNI GTAC 4 cut(s) 193, 830, 883, 1169
SaqAI TTAA 4 cut(s) 8, 300, 312, 1029
SatI GCNGC 3 cut(s) 149, 894, 950
Sau3AI GATC 3 cut(s) 270, 466, 1120
Sau96I GGNCC 1 cut(s) 54
ScaI AGTACT 1 cut(s) 1170
SchI GAGTC 1 cut(s) 489
ScrFI CCNGG 2 cut(s) 231, 348
SduI GDGCHC 2 cut(s) 814, 1066
SfaNI GCATC 1 cut(s) 705
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
SnaBI TACGTA 1 cut(s) 1198
SpeI ACTAGT 2 cut(s) 449, 755
Sse9I AATT 7 cut(s) 64, 319, 340, 527, 598, 1091, 1205
SsiI CCGC 3 cut(s) 248, 443, 1118
SspMI CTAG 5 cut(s) 450, 756, 789, 848, 1067
StyD4I CCNGG 2 cut(s) 229, 346
TaaI ACNGT 3 cut(s) 638, 870, 959
TaiI ACGT 3 cut(s) 720, 1106, 1200
TaqI TCGA 3 cut(s) 143, 269, 975
TasI AATT 7 cut(s) 64, 319, 340, 527, 598, 1091, 1205
TatI WGTACW 2 cut(s) 882, 1168
TfiI GAWTC 4 cut(s) 81, 201, 266, 417
Tru1I TTAA 4 cut(s) 8, 300, 312, 1029
Tru9I TTAA 4 cut(s) 8, 300, 312, 1029
TscAI CASTG 5 cut(s) 246, 481, 736, 814, 875
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 3 cut(s) 148, 893, 949
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 388, 613
TspGWI ACGGA 1 cut(s) 221
TspRI CASTG 5 cut(s) 246, 481, 736, 814, 875
VneI GTGCAC 1 cut(s) 810
VpaK11BI GGWCC 1 cut(s) 54
XapI RAATTY 2 cut(s) 64, 340
XceI RCATGY 2 cut(s) 568, 931
XmnI GAANNNNTTC 3 cut(s) 355, 566, 902
XspI CTAG 5 cut(s) 450, 756, 789, 848, 1067
ZraI GACGTC 1 cut(s) 718
ZrmI AGTACT 1 cut(s) 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.