MD15G1009500.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001158
Physical Location & Seq
Forward (+)
505788 .. 508211
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1009500.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGGAGTCTTCTTCTTCTTGTTCTTCCCAGTTGCTGTCGTCCCGGTTGATATTATGCGTTTGTGTTGTAGTTTTTGGTGTTTTCCAGTACCCTACCGCCACAGAGGGACTGGTGATGCTGCCATCAAACCAGACCTTTCCGGCGGTGATTGTGTTTGGCGATTCGATCATGGACACCGGCAACAACAACCATCTTAAGTCACTTGTTAAGTGCAATTTCTCTCCTTATGGACGAGATTTCCAGCAGCACAAGCCTACTGGCAGGTTTGGCAATGGCAAGGTGCCCTCTGACATCATAGCGTCACAATTGGGAATCCAAGAGTATTTGCCGGCATATTTGGATCCAAATCTACGACCGCAAGATCTCCCCGCGGGAGTTGTCTTTGCTTCAGGTGGCACCGGATATGATCCAATGACACCCCAGATAGTGTCAGTTATATCAATGGATGATCAGTTGAAAATGTTCAAAGAGTATATCGTGAAGCTCAAAGGCGTTGTTGGAGAAGAGAGAACCCAGTTCATTCTATCCAACGCTCTAGTTCTAGTGGTTGCCGGCAGCGATGACATCGCCAACACTTATTTTACCATTCGCATAAGAAAAGCTCAATATAGTGTTCCTGCTTACACTGACCTCATGGTCAACTCTGCATCTAGTTTCGTCAAGGAATTGTATAAACTTGGGGCGAGAAGGATCGGCTTGTTCAGTGCACCGCCAATAGGATGTGTGCCTTCACAAAGAACTTTGGGTGGAGGAATTCTGAGAATGTGTGCAGAAGAATACAACGATGCGGCAAAGTTATTCAACATCAAACTGTCCAGCTCACTCAACTCCTTGAGCTCCGCCCTGCCCCACAGTAAAATCGTCTATGTGGATATTTACAATCCACTGCTCGATATAATTCTCAACCCTGCCAAAAGTGGGTTTAAAGTTGTGGAGAAAGGTTGCTGTGGAACGGGGAACATAGAGGTAGCAGTGTTGTGCAACAAGTTCGATTCTACGTGTGAAAATCCATCCGATTATGTGTTTTGGGACAGTTATCATCCTACAGAACGAACCTACAATGTACTCATTCCTCCACTTCTTCAGAGATACATTAAAGACTTCTTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

371

Amino Acids

40.7

Weight (kDa)

6.87

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 49 - 355 7.2e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 252
AccB1I GGYRCC 2 cut(s) 280, 395
AccII CGCG 1 cut(s) 371
AciI CCGC 8 cut(s) 96, 143, 356, 369, 371, 710, 788, 840
AclWI GGATC 4 cut(s) 335, 348, 401, 698
AcsI RAATTY 1 cut(s) 753
AcuI CTGAAG 2 cut(s) 372, 1067
AfaI GTAC 2 cut(s) 89, 1065
AfiI CCNNNNNNNGG 2 cut(s) 716, 918
AflII CTTAAG 1 cut(s) 194
AflIII ACRYGT 1 cut(s) 998
AgsI TTSAA 3 cut(s) 457, 466, 802
AluBI AGCT 4 cut(s) 484, 602, 819, 837
AluI AGCT 4 cut(s) 484, 602, 819, 837
Alw21I GWGCWC 2 cut(s) 709, 839
Alw44I GTGCAC 1 cut(s) 705
AlwI GGATC 4 cut(s) 335, 348, 401, 698
AlwNI CAGNNNCTG 1 cut(s) 34
ApaLI GTGCAC 1 cut(s) 705
ApeKI GCWGC 3 cut(s) 118, 244, 555
ApoI RAATTY 1 cut(s) 753
Asp700I GAANNNNTTC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 2 cut(s) 124, 157
BaeGI GKGCMC 2 cut(s) 285, 709
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 2 cut(s) 280, 395
BanII GRGCYC 1 cut(s) 839
Bbv12I GWGCWC 2 cut(s) 709, 839
BbvI GCAGC 3 cut(s) 105, 256, 567
BccI CCATC 3 cut(s) 130, 198, 1018
BcgI CGANNNNNNTGC 2 cut(s) 970, 1004
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 43
BfaI CTAG 3 cut(s) 536, 542, 651
BfmI CTRYAG 1 cut(s) 1044
BfrI CTTAAG 1 cut(s) 194
BfuAI ACCTGC 1 cut(s) 252
BglII AGATCT 1 cut(s) 361
BisI GCNGC 4 cut(s) 119, 245, 556, 789
BlsI GCNGC 4 cut(s) 120, 246, 557, 790
Bme1390I CCNGG 1 cut(s) 43
BmiI GGNNCC 3 cut(s) 282, 342, 397
BmrFI CCNGG 1 cut(s) 43
BmrI ACTGGG 2 cut(s) 22, 508
BmsI GCATC 3 cut(s) 105, 656, 775
BmuI ACTGGG 2 cut(s) 22, 508
BpuEI CTTGAG 1 cut(s) 853
BpuMI CCSGG 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 999
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 369
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 2 cut(s) 716, 918
Bse118I RCCGGY 3 cut(s) 176, 328, 551
Bse1I ACTGG 5 cut(s) 28, 85, 114, 262, 514
Bse3DI GCAATG 1 cut(s) 277
Bse8I GATNNNNATC 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 4 cut(s) 451, 725, 1010, 1039
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 716, 918
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 749
BseNI ACTGG 5 cut(s) 28, 85, 114, 262, 514
BseSI GKGCMC 2 cut(s) 285, 709
BseXI GCAGC 3 cut(s) 105, 256, 567
BsgI GTGCAG 1 cut(s) 789
Bsh1236I CGCG 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 356
BshNI GGYRCC 2 cut(s) 280, 395
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 2 cut(s) 709, 839
BsiSI CCGG 6 cut(s) 43, 140, 177, 329, 399, 552
BslFI GGGAC 3 cut(s) 25, 120, 1043
BslI CCNNNNNNNGG 2 cut(s) 716, 918
BsmFI GGGAC 3 cut(s) 25, 120, 1043
Bsp1286I GDGCHC 3 cut(s) 285, 709, 839
Bsp143I GATC 6 cut(s) 165, 340, 361, 406, 448, 690
BspACI CCGC 8 cut(s) 96, 143, 356, 369, 371, 710, 788, 840
BspCNI CTCAG 1 cut(s) 750
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 3 cut(s) 282, 342, 397
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 4 cut(s) 335, 348, 401, 698
BspT107I GGYRCC 2 cut(s) 280, 395
BspTI CTTAAG 1 cut(s) 194
BsrDI GCAATG 1 cut(s) 277
BsrFI RCCGGY 3 cut(s) 176, 328, 551
BsrI ACTGG 5 cut(s) 28, 85, 114, 262, 514
BssAI RCCGGY 3 cut(s) 176, 328, 551
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 6 cut(s) 165, 340, 361, 406, 448, 690
Bst4CI ACNGT 3 cut(s) 813, 854, 1034
Bst6I CTCTTC 1 cut(s) 498
BstAFI CTTAAG 1 cut(s) 194
BstBAI YACGTR 1 cut(s) 999
BstC8I GCNNGC 2 cut(s) 330, 553
BstDEI CTNAG 1 cut(s) 758
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 4 cut(s) 451, 725, 1010, 1039
BstFNI CGCG 1 cut(s) 371
BstKTI GATC 6 cut(s) 168, 343, 364, 409, 451, 693
BstMBI GATC 6 cut(s) 165, 340, 361, 406, 448, 690
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 2 cut(s) 250, 267
BstSCI CCNGG 1 cut(s) 41
BstSFI CTRYAG 1 cut(s) 1044
BstSLI GKGCMC 2 cut(s) 285, 709
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 3 cut(s) 105, 256, 567
BstX2I RGATCY 2 cut(s) 340, 361
BstYI RGATCY 2 cut(s) 340, 361
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 2 cut(s) 550, 573
BtsCI GGATG 4 cut(s) 451, 725, 1010, 1039
BtsI GCAGTG 2 cut(s) 884, 978
BtsIMutI CAGTG 4 cut(s) 624, 709, 884, 978
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 2 cut(s) 330, 553
CaiI CAGNNNCTG 1 cut(s) 34
Cfr10I RCCGGY 3 cut(s) 176, 328, 551
Cfr42I CCGCGG 1 cut(s) 372
CseI GACGC 1 cut(s) 288
Csp6I GTAC 2 cut(s) 88, 1064
CviAII CATG 2 cut(s) 169, 634
CviJI RGCY 6 cut(s) 253, 484, 602, 696, 819, 837
CviKI_1 RGCY 6 cut(s) 253, 484, 602, 696, 819, 837
CviQI GTAC 2 cut(s) 88, 1064
DdeI CTNAG 1 cut(s) 758
DpnI GATC 6 cut(s) 167, 342, 363, 408, 450, 692
DpnII GATC 6 cut(s) 165, 340, 361, 406, 448, 690
DraI TTTAAA 1 cut(s) 925
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 1 cut(s) 829
Ecl136II GAGCTC 1 cut(s) 837
Eco24I GRGCYC 1 cut(s) 839
Eco53kI GAGCTC 1 cut(s) 837
Eco57I CTGAAG 2 cut(s) 372, 1067
EcoICRI GAGCTC 1 cut(s) 837
EcoRI GAATTC 1 cut(s) 753
EcoT38I GRGCYC 1 cut(s) 839
FaeI CATG 2 cut(s) 172, 637
FaqI GGGAC 3 cut(s) 25, 120, 1043
FatI CATG 2 cut(s) 168, 633
FauI CCCGC 2 cut(s) 364, 376
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 4 cut(s) 119, 245, 556, 789
FokI GGATG 4 cut(s) 458, 732, 997, 1026
FriOI GRGCYC 1 cut(s) 839
Fsp4HI GCNGC 4 cut(s) 119, 245, 556, 789
FspBI CTAG 3 cut(s) 536, 542, 651
GluI GCNGC 4 cut(s) 119, 245, 556, 789
HapII CCGG 6 cut(s) 43, 140, 177, 329, 399, 552
HgaI GACGC 1 cut(s) 288
Hin1II CATG 2 cut(s) 172, 637
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HinfI GANTC 4 cut(s) 5, 161, 312, 992
HpaII CCGG 6 cut(s) 43, 140, 177, 329, 399, 552
HphI GGTGA 2 cut(s) 124, 157
Hpy166II GTNNAC 2 cut(s) 640, 707
Hpy188I TCNGA 4 cut(s) 289, 759, 1015, 1086
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 2 cut(s) 640, 707
HpyAV CCTTC 2 cut(s) 681, 738
HpyCH4III ACNGT 3 cut(s) 813, 854, 1034
HpyCH4IV ACGT 1 cut(s) 998
HpyCH4V TGCA 5 cut(s) 213, 647, 707, 770, 981
HpyF10VI GCNNNNNNNGC 2 cut(s) 250, 267
HpyF3I CTNAG 1 cut(s) 758
HpySE526I ACGT 1 cut(s) 998
Hsp92II CATG 2 cut(s) 172, 637
KroI GCCGGC 2 cut(s) 328, 551
KroNI GCCGGC 2 cut(s) 330, 553
Ksp22I TGATCA 1 cut(s) 448
KspI CCGCGG 1 cut(s) 372
Kzo9I GATC 6 cut(s) 165, 340, 361, 406, 448, 690
LmnI GCTCC 1 cut(s) 842
Lsp1109I GCAGC 3 cut(s) 105, 256, 567
LweI GCATC 3 cut(s) 105, 656, 775
MaeI CTAG 3 cut(s) 536, 542, 651
MaeII ACGT 1 cut(s) 998
MaeIII GTNAC 2 cut(s) 198, 300
MalI GATC 6 cut(s) 167, 342, 363, 408, 450, 692
MboI GATC 6 cut(s) 165, 340, 361, 406, 448, 690
MboII GAAGA 6 cut(s) 3, 6, 15, 515, 785, 1073
MfeI CAATTG 1 cut(s) 305
MflI RGATCY 2 cut(s) 340, 361
MhlI GDGCHC 3 cut(s) 285, 709, 839
MluCI AATT 5 cut(s) 214, 305, 665, 753, 897
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 478, 552
MnlI CCTC 6 cut(s) 97, 295, 641, 743, 958, 1083
MroNI GCCGGC 2 cut(s) 328, 551
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 5 cut(s) 195, 207, 924, 1095, 1111
MspA1I CMGCKG 1 cut(s) 371
MspCI CTTAAG 1 cut(s) 194
MspI CCGG 6 cut(s) 43, 140, 177, 329, 399, 552
MspR9I CCNGG 1 cut(s) 43
MunI CAATTG 1 cut(s) 305
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 2 cut(s) 250, 267
NaeI GCCGGC 2 cut(s) 330, 553
NciI CCSGG 1 cut(s) 43
NdeII GATC 6 cut(s) 165, 340, 361, 406, 448, 690
NgoMIV GCCGGC 2 cut(s) 328, 551
NlaIII CATG 2 cut(s) 172, 637
NlaIV GGNNCC 3 cut(s) 282, 342, 397
NmuCI GTSAC 2 cut(s) 198, 300
PdiI GCCGGC 2 cut(s) 330, 553
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 3 cut(s) 161, 312, 992
PkrI GCNGC 4 cut(s) 120, 246, 557, 790
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 999
Psp124BI GAGCTC 1 cut(s) 839
PspN4I GGNNCC 3 cut(s) 282, 342, 397
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 2 cut(s) 340, 361
RsaI GTAC 2 cut(s) 89, 1065
RsaNI GTAC 2 cut(s) 88, 1064
SacI GAGCTC 1 cut(s) 839
SacII CCGCGG 1 cut(s) 372
SaqAI TTAA 5 cut(s) 195, 207, 924, 1095, 1111
SatI GCNGC 4 cut(s) 119, 245, 556, 789
Sau3AI GATC 6 cut(s) 165, 340, 361, 406, 448, 690
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 3 cut(s) 285, 709, 839
SfaNI GCATC 3 cut(s) 105, 656, 775
SfcI CTRYAG 1 cut(s) 1044
Sfr303I CCGCGG 1 cut(s) 372
SgrBI CCGCGG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 194, 832
SmoI CTYRAG 2 cut(s) 194, 832
Sse9I AATT 5 cut(s) 214, 305, 665, 753, 897
SsiI CCGC 8 cut(s) 96, 143, 356, 369, 371, 710, 788, 840
SspMI CTAG 3 cut(s) 536, 542, 651
SstI GAGCTC 1 cut(s) 839
StyD4I CCNGG 1 cut(s) 41
TaaI ACNGT 3 cut(s) 813, 854, 1034
TaiI ACGT 1 cut(s) 1001
TaqI TCGA 3 cut(s) 164, 891, 990
TasI AATT 5 cut(s) 214, 305, 665, 753, 897
TatI WGTACW 1 cut(s) 1063
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 3 cut(s) 161, 312, 992
Tru1I TTAA 5 cut(s) 195, 207, 924, 1095, 1111
Tru9I TTAA 5 cut(s) 195, 207, 924, 1095, 1111
TscAI CASTG 4 cut(s) 631, 709, 891, 978
TseFI GTSAC 2 cut(s) 198, 300
TseI GCWGC 3 cut(s) 118, 244, 555
Tsp45I GTSAC 2 cut(s) 198, 300
TspDTI ATGAA 1 cut(s) 508
TspRI CASTG 4 cut(s) 631, 709, 891, 978
Vha464I CTTAAG 1 cut(s) 194
VneI GTGCAC 1 cut(s) 705
XapI RAATTY 1 cut(s) 753
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 3 cut(s) 536, 542, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.