Rh6AG435400

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001158
Physical Location & Seq
Forward (+)
62494604 .. 62498137
3534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG435400.1

Sequence Viewer

Length: 1101 bp
ATGGATGCAGCACGAGCAATAGTACTACCGTGTATTCTTTGTTATATTCTGATGTGCAATGATAGCGTCGGAGAGGATTTAACCCTGAAGCAGATGAAGCTTAAAATACCAGCGAACGTAAGTGTTCCGGCAGTGATTGTGTTTGGAGATTCAATAGTTGATACGGGCAACAATAACAACAACTTCATAACATTTGCTCGCTGCAATTTCCTCCCTTATGGCATAGACTTCCAAGGTGGACGTGCAACCGGACGATTCTCAAATGGAAAGGTTCCTGCTGACCTTGTAGTGGAAGCATTTGGAATTAAGGAGCTGTTGCCACCCTATATGGATCCATCCCTAGAGCCTAATGATCTCCTTACTGGTGTTAACTTTGCTGCTGGAGGTGTAGGTTATGACCCTTTGACATCAGAAGTCGCGGCAGTTGAATCACTGTCCGATCAGATGGAACAATTCAAAGGGTACATAGAAAAGCTGAAAGGAATCGTCGGAGAAAAGAGAACAAACTTCATCATATCAAATAGTTTGATTTTAGTGGTAGCAGGAAGCAACGACATTTCGAGTACCTATTTTGTTAGTGGTATCAGGAAATTGCATTTCGATGTTCCTTCTTACACTGACTTCTTGCTCAACCATGCTTCGCAATTCTTCAAGGAATTATATGGATTGGGTGCACGAAGAATAGGCGTTTTTGGTGTACCAGCTATTGGGTGTGTGCCATCCCAAAGAACAATTGGAGGAGGCATCGGAAGACATTGTGCCGAGAATCAAAACCAAGCAGCGGAGCTCTTCAACTCCAAATTATCTGCAGAGACGAATCACCTCAGACACACACTCCCCAACTCAAAGTTCGTTTACATAGACGTCTACAACCCATTACTTGATATCATCACAAACTCTAATAAATACGGGTTTGAAGTTGCAGACAAAGGGTGTTGTGGTACTGGTTTGGTGGAAGTGACAAAACTGTGCAACAAGTTTCAGCGAACCTGTACGGACACCTCCAAATATGTCTTTTGGGATAGTTACCATCCAACTGAAAGAGCAAACAGGATCCTTCTGCAGCAACTCCTAACGCAATCTGCAAGCGGTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.09

Weight (kDa)

6.1

Isoelectric Point (pI)

23.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 355 9.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 867
AccB7I CCANNNNNTGG 1 cut(s) 707
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 419
AciI CCGC 3 cut(s) 419, 782, 1089
AclWI GGATC 4 cut(s) 326, 339, 1048, 1061
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 864
AfaI GTAC 6 cut(s) 24, 464, 565, 699, 943, 994
AfiI CCNNNNNNNGG 3 cut(s) 289, 707, 781
AgsI TTSAA 6 cut(s) 153, 428, 457, 652, 793, 917
AjiI CACGTC 1 cut(s) 242
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
AluBI AGCT 5 cut(s) 100, 313, 475, 704, 787
AluI AGCT 5 cut(s) 100, 313, 475, 704, 787
Alw21I GWGCWC 2 cut(s) 676, 789
Alw26I GTCTC 1 cut(s) 806
Alw44I GTGCAC 1 cut(s) 672
AlwI GGATC 4 cut(s) 326, 339, 1048, 1061
ApaLI GTGCAC 1 cut(s) 672
ApeKI GCWGC 5 cut(s) 8, 201, 377, 779, 1063
AsuHPI GGTGA 1 cut(s) 812
BaeGI GKGCMC 1 cut(s) 676
BamHI GGATCC 2 cut(s) 331, 1053
BanII GRGCYC 1 cut(s) 789
BauI CACGAG 1 cut(s) 12
BbsI GAAGAC 1 cut(s) 757
Bbv12I GWGCWC 2 cut(s) 676, 789
BbvI GCAGC 5 cut(s) 20, 188, 364, 791, 1075
BccI CCATC 4 cut(s) 343, 439, 727, 1038
BcoDI GTCTC 1 cut(s) 806
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 2 cut(s) 807, 1061
BisI GCNGC 6 cut(s) 9, 202, 378, 420, 780, 1064
BlsI GCNGC 6 cut(s) 10, 203, 379, 421, 781, 1065
BmcAI AGTACT 1 cut(s) 24
BmgBI CACGTC 1 cut(s) 242
BmiI GGNNCC 3 cut(s) 273, 333, 1055
BmsI GCATC 1 cut(s) 753
BpiI GAAGAC 1 cut(s) 757
BpmI CTGGAG 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 864
BsaJI CCNNGG 1 cut(s) 232
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 3 cut(s) 289, 707, 781
Bse1I ACTGG 2 cut(s) 367, 949
Bse3DI GCAATG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 232
BseGI GGATG 4 cut(s) 10, 335, 719, 1030
BseLI CCNNNNNNNGG 3 cut(s) 289, 707, 781
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 1 cut(s) 838
BseNI ACTGG 2 cut(s) 367, 949
BseRI GAGGAG 1 cut(s) 753
BseSI GKGCMC 1 cut(s) 676
BseXI GCAGC 5 cut(s) 20, 188, 364, 791, 1075
Bsh1236I CGCG 1 cut(s) 419
BsiHKAI GWGCWC 2 cut(s) 676, 789
BsiSI CCGG 2 cut(s) 128, 249
BslI CCNNNNNNNGG 3 cut(s) 289, 707, 781
BsmAI GTCTC 1 cut(s) 806
BsmBI CGTCTC 1 cut(s) 806
Bsp1286I GDGCHC 2 cut(s) 676, 789
Bsp143I GATC 4 cut(s) 331, 352, 439, 1053
BspACI CCGC 3 cut(s) 419, 782, 1089
BspCNI CTCAG 1 cut(s) 837
BspFNI CGCG 1 cut(s) 419
BspLI GGNNCC 3 cut(s) 273, 333, 1055
BspMAI CTGCAG 2 cut(s) 811, 1065
BspPI GGATC 4 cut(s) 326, 339, 1048, 1061
BspQI GCTCTTC 1 cut(s) 794
BsrDI GCAATG 1 cut(s) 64
BsrI ACTGG 2 cut(s) 367, 949
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 4 cut(s) 331, 352, 439, 1053
BssNI GRCGYC 1 cut(s) 864
BssSI CACGAG 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 30, 435, 969
Bst6I CTCTTC 1 cut(s) 794
BstACI GRCGYC 1 cut(s) 864
BstC8I GCNNGC 2 cut(s) 199, 1087
BstDEI CTNAG 1 cut(s) 824
BstF5I GGATG 4 cut(s) 10, 335, 719, 1030
BstFNI CGCG 1 cut(s) 419
BstKTI GATC 4 cut(s) 334, 355, 442, 1056
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 4 cut(s) 331, 352, 439, 1053
BstMWI GCNNNNNNNGC 3 cut(s) 14, 63, 97
BstSFI CTRYAG 2 cut(s) 807, 1061
BstSLI GKGCMC 1 cut(s) 676
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 5 cut(s) 20, 188, 364, 791, 1075
BstV2I GAAGAC 1 cut(s) 757
BstX2I RGATCY 2 cut(s) 331, 1053
BstYI RGATCY 2 cut(s) 331, 1053
BtrI CACGTC 1 cut(s) 242
BtsCI GGATG 4 cut(s) 10, 335, 719, 1030
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 3 cut(s) 138, 431, 615
Cac8I GCNNGC 2 cut(s) 199, 1087
CseI GACGC 1 cut(s) 55
Csp6I GTAC 6 cut(s) 23, 463, 564, 698, 942, 993
CviAII CATG 1 cut(s) 635
CviJI RGCY 6 cut(s) 100, 313, 346, 475, 704, 787
CviKI_1 RGCY 6 cut(s) 100, 313, 346, 475, 704, 787
CviQI GTAC 6 cut(s) 23, 463, 564, 698, 942, 993
DdeI CTNAG 1 cut(s) 824
DpnI GATC 4 cut(s) 333, 354, 441, 1055
DpnII GATC 4 cut(s) 331, 352, 439, 1053
Eam1104I CTCTTC 1 cut(s) 794
EarI CTCTTC 1 cut(s) 794
Ecl136II GAGCTC 1 cut(s) 787
Eco130I CCWWGG 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 789
Eco32I GATATC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 787
Eco57I CTGAAG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 787
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 789
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 806
FaeI CATG 1 cut(s) 638
FatI CATG 1 cut(s) 634
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 6 cut(s) 9, 202, 378, 420, 780, 1064
FokI GGATG 4 cut(s) 17, 322, 706, 1017
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 6 cut(s) 9, 202, 378, 420, 780, 1064
FspBI CTAG 1 cut(s) 341
GluI GCNGC 6 cut(s) 9, 202, 378, 420, 780, 1064
GsuI CTGGAG 1 cut(s) 402
HapII CCGG 2 cut(s) 128, 249
HgaI GACGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 864
Hin1II CATG 1 cut(s) 638
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 6 cut(s) 149, 255, 428, 483, 766, 817
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 2 cut(s) 128, 249
HphI GGTGA 1 cut(s) 812
Hpy166II GTNNAC 6 cut(s) 239, 370, 674, 698, 856, 868
Hpy188I TCNGA 8 cut(s) 51, 71, 412, 439, 444, 491, 749, 827
Hpy188III TCNNGA 1 cut(s) 586
Hpy8I GTNNAC 6 cut(s) 239, 370, 674, 698, 856, 868
Hpy99I CGWCG 2 cut(s) 71, 491
HpyAV CCTTC 2 cut(s) 618, 1067
HpyCH4III ACNGT 3 cut(s) 30, 435, 969
HpyCH4IV ACGT 3 cut(s) 117, 241, 864
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 63, 97
HpyF3I CTNAG 1 cut(s) 824
HpySE526I ACGT 3 cut(s) 117, 241, 864
Hsp92I GRCGYC 1 cut(s) 864
Hsp92II CATG 1 cut(s) 638
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 4 cut(s) 331, 352, 439, 1053
LguI GCTCTTC 1 cut(s) 794
LmnI GCTCC 2 cut(s) 310, 784
Lsp1109I GCAGC 5 cut(s) 20, 188, 364, 791, 1075
LweI GCATC 1 cut(s) 753
MaeI CTAG 1 cut(s) 341
MaeII ACGT 3 cut(s) 117, 241, 864
MaeIII GTNAC 2 cut(s) 958, 1025
MalI GATC 4 cut(s) 333, 354, 441, 1055
MboI GATC 4 cut(s) 331, 352, 439, 1053
MboII GAAGA 4 cut(s) 640, 690, 762, 781
MfeI CAATTG 1 cut(s) 732
MflI RGATCY 2 cut(s) 331, 1053
MhlI GDGCHC 2 cut(s) 676, 789
MluCI AATT 8 cut(s) 205, 303, 452, 590, 644, 656, 732, 800
MmeI TCCRAC 3 cut(s) 49, 469, 1058
MnlI CCTC 7 cut(s) 67, 221, 377, 731, 734, 833, 1012
MseI TTAA 4 cut(s) 80, 102, 306, 369
MslI CAYNNNNRTG 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 2 cut(s) 128, 249
MunI CAATTG 1 cut(s) 732
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 3 cut(s) 14, 63, 97
NdeII GATC 4 cut(s) 331, 352, 439, 1053
NlaIII CATG 1 cut(s) 638
NlaIV GGNNCC 3 cut(s) 273, 333, 1055
NmeAIII GCCGAG 1 cut(s) 787
NmuCI GTSAC 1 cut(s) 958
PciSI GCTCTTC 1 cut(s) 794
PfeI GAWTC 6 cut(s) 149, 255, 428, 483, 766, 817
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 6 cut(s) 10, 203, 379, 421, 781, 1065
Psp124BI GAGCTC 1 cut(s) 789
PspN4I GGNNCC 3 cut(s) 273, 333, 1055
PstI CTGCAG 2 cut(s) 811, 1065
PsuI RGATCY 2 cut(s) 331, 1053
RsaI GTAC 6 cut(s) 24, 464, 565, 699, 943, 994
RsaNI GTAC 6 cut(s) 23, 463, 564, 698, 942, 993
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 789
SapI GCTCTTC 1 cut(s) 794
SaqAI TTAA 4 cut(s) 80, 102, 306, 369
SatI GCNGC 6 cut(s) 9, 202, 378, 420, 780, 1064
Sau3AI GATC 4 cut(s) 331, 352, 439, 1053
ScaI AGTACT 1 cut(s) 24
SduI GDGCHC 2 cut(s) 676, 789
SfaNI GCATC 1 cut(s) 753
SfcI CTRYAG 2 cut(s) 807, 1061
SmiMI CAYNNNNRTG 1 cut(s) 600
Sse9I AATT 8 cut(s) 205, 303, 452, 590, 644, 656, 732, 800
SsiI CCGC 3 cut(s) 419, 782, 1089
SspMI CTAG 1 cut(s) 341
SstI GAGCTC 1 cut(s) 789
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 3 cut(s) 30, 435, 969
TaiI ACGT 3 cut(s) 120, 244, 867
TaqI TCGA 2 cut(s) 560, 600
TasI AATT 8 cut(s) 205, 303, 452, 590, 644, 656, 732, 800
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 6 cut(s) 149, 255, 428, 483, 766, 817
Tru1I TTAA 4 cut(s) 80, 102, 306, 369
Tru9I TTAA 4 cut(s) 80, 102, 306, 369
TscAI CASTG 3 cut(s) 138, 438, 622
TseFI GTSAC 1 cut(s) 958
TseI GCWGC 5 cut(s) 8, 201, 377, 779, 1063
Tsp45I GTSAC 1 cut(s) 958
TspDTI ATGAA 3 cut(s) 110, 175, 499
TspGWI ACGGA 1 cut(s) 1010
TspRI CASTG 3 cut(s) 138, 438, 622
Van91I CCANNNNNTGG 1 cut(s) 707
VneI GTGCAC 1 cut(s) 672
XcmI CCANNNNNNNNNTGG 1 cut(s) 731
XmiI GTMKAC 1 cut(s) 867
XspI CTAG 1 cut(s) 341
ZraI GACGTC 1 cut(s) 865
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.