AT5G64500

sphingolipid transporter spinster homolog 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001054
Physical Location & Seq
Forward (+)
25780382 .. 25783753
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64500.1

Sequence Viewer

Length: 1455 bp
ATGGATGTTGACGGAGAAGGTGATCGTGGTCAAAACCCTAGAATTATGGAGAGGGACTCAGATTCTATCAAAGATCCGATCTCAGAGCCTTCATGGTTTACCCCCAAAAAGCTTCTCTTTGTGTTTTGTGTTGTCAACTTAATCAATTATATAGATCGAGGAGCTATAGCAAGTAATGGTATCAATGGGAGTCGTGGTTCTTGCACTTCGAGTGGTACATGTTCATCTGGCAGCGGAATACAGGGAGATTTCAACTTGAGCAATTTTGAAGATGGAGTTCTGTCATCTGCTTTCATGGTTGGGCTTCTTGTTGCCTCTCCTATATTTGCTTCCTTAGCTAAGAGTGTTAATCCGTTTAGACTTATCGGAGTTGGGTTGTCAATCTGGACTCTCGCTGTGATTGGCTGCGGTCTTTCGTTTGATTTTTGGTCCATCACAATCTGTCGCATGTTTGTTGGTGTTGGTGAGGCATCATTTGTAAGTCTTGCTGCCCCATTCATCGACGATAATGCCCCACATGATCAGAAATCAGCGTGGCTTGCTGTATTCTACATGTGCATTCCGACAGGCTATGCTTTTGGCTACGTATATGGTGGAGTGGTGGGGAGTGTCCTTCCTTGGCGTGCAGCATTTTGGGGAGAAGCAATACTTATGCTTCCTTTTGCTGTTCTAGGTTTTGTCATCAAACCCTTGCATCTGAAAGGTTTTGCTCCAGATGATACAGGAAAGCCCCGGACAGATAACTTAAACGTTTTGCCAGTTGGTTATGGGTTCTCAGCTGTTATGAAAGATTTGAAATTGCTTTTGGTAGATAAGGTTTATGTTACAAACATTCTTGGGTATATTGCTTACAACTTTGTTTTAGGCGCCTATTCATATTGGGGACCAAAGGCTGGTTATAACATATACAAGATGGAAAACGCTGATATGATATTTGGAGGGGTCACAGTTGTATGCGGAATTGTTGGCACATTATCAGGAGGAGTGATTCTAGATTACATGGATGCCACAATTTCAAATGCTTTTAAGGTTCTTTCAGTTTCAACGTTTATAGGAGCAATATTTTGCTTTGCTGCTTTCTGTTTCAAGAGCATGTATGCATTTTTAGCTCTTTTTGCTGTTGGTGAACTTCTTGTCTTTGCCACACAGGGTCCTGTGAATTTCATAGTTCTCCATTGTGTCAAACCAAGTCTGAGACCACTTGCAATGGCGATGTCTACTGTCTCTATCCATATCTTTGGAGATGTACCTTCCTCACCACTCGTTGGAGTTCTTCAGGACTACGTGAACAATTGGAGAGTGACTTCTCTTGTTCTTACATTCGTTCTCTTTCCAGCTGCTGCAATTTGGTCCATAGGGATTTTCCTGAATAGTGTGGATCGATATAACGAAGATTCCGAGCCTGATGCAGTAACCAGAGAATCAACCGCTGCGCCTCTTCTCCAGGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

484

Amino Acids

52.15

Weight (kDa)

4.99

Isoelectric Point (pI)

23.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 79 - 405 2.2e-28 Major Facilitator Superfamily
OATP PF03137 145 - 201 1.1e-07 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 258 - 361 6.1e-09 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 360 - 427 2.1e-07 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 900
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 2 cut(s) 893, 1265
AccI GTMKAC 1 cut(s) 1217
AciI CCGC 4 cut(s) 234, 408, 957, 1428
AclI AACGTT 2 cut(s) 750, 1046
AclWI GGATC 2 cut(s) 68, 1386
AcsI RAATTY 1 cut(s) 1159
AcuI CTGAAG 1 cut(s) 1259
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 2 cut(s) 217, 1248
AfiI CCNNNNNNNGG 2 cut(s) 893, 1265
AflIII ACRYGT 2 cut(s) 218, 552
AgsI TTSAA 6 cut(s) 253, 269, 796, 1017, 1044, 1087
AjnI CCWGG 1 cut(s) 1443
AjuI GAANNNNNNNTTGG 2 cut(s) 788, 820
AloI GAACNNNNNNTCC 2 cut(s) 181, 213
AluBI AGCT 6 cut(s) 112, 164, 338, 779, 1109, 1337
AluI AGCT 6 cut(s) 112, 164, 338, 779, 1109, 1337
Alw26I GTCTC 2 cut(s) 1189, 1228
AlwI GGATC 2 cut(s) 68, 1386
AlwNI CAGNNNCTG 1 cut(s) 1340
ApeKI GCWGC 8 cut(s) 231, 405, 488, 626, 1073, 1337, 1340, 1430
ApoI RAATTY 1 cut(s) 1159
AspLEI GCGC 2 cut(s) 869, 1435
AspS9I GGNCC 4 cut(s) 429, 884, 1151, 1350
AsuC2I CCSGG 1 cut(s) 733
AsuHPI GGTGA 4 cut(s) 32, 476, 1136, 1248
AvaII GGWCC 4 cut(s) 429, 884, 1151, 1350
BanI GGYRCC 1 cut(s) 866
BbvI GCAGC 8 cut(s) 243, 392, 475, 638, 1060, 1324, 1327, 1417
BccI CCATC 3 cut(s) 266, 440, 907
BcgI CGANNNNNNTGC 4 cut(s) 491, 525, 1388, 1422
BciT130I CCWGG 1 cut(s) 1445
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 1 cut(s) 733
BcoDI GTCTC 2 cut(s) 1189, 1228
BfaI CTAG 3 cut(s) 39, 671, 992
BfmI CTRYAG 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 870
BisI GCNGC 8 cut(s) 232, 406, 489, 627, 1074, 1338, 1341, 1431
BlsI GCNGC 8 cut(s) 233, 407, 490, 628, 1075, 1339, 1342, 1432
Bme1390I CCNGG 2 cut(s) 733, 1445
Bme18I GGWCC 4 cut(s) 429, 884, 1151, 1350
BmgT120I GGNCC 4 cut(s) 429, 884, 1151, 1350
BmiI GGNNCC 3 cut(s) 868, 885, 1152
BmrFI CCNGG 2 cut(s) 733, 1445
BmsI GCATC 4 cut(s) 479, 703, 994, 1396
BplI GAGNNNNNCTC 2 cut(s) 41, 73
BpmI CTGGAG 2 cut(s) 696, 1427
Bpu10I CCTNAGC 1 cut(s) 334
BpuEI CTTGAG 1 cut(s) 277
BpuMI CCSGG 1 cut(s) 733
Bsa29I ATCGAT 1 cut(s) 1381
BsaAI YACGTR 2 cut(s) 586, 1285
BsaHI GRCGYC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 1189
BsaJI CCNNGG 2 cut(s) 617, 731
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 2 cut(s) 893, 1265
Bse1I ACTGG 1 cut(s) 758
Bse3DI GCAATG 1 cut(s) 1212
BseBI CCWGG 1 cut(s) 1445
BseCI ATCGAT 1 cut(s) 1381
BseDI CCNNGG 2 cut(s) 617, 731
BseGI GGATG 2 cut(s) 10, 1009
BseLI CCNNNNNNNGG 2 cut(s) 893, 1265
BseMI GCAATG 1 cut(s) 1212
BseMII CTCAG 4 cut(s) 72, 96, 789, 1184
BseNI ACTGG 1 cut(s) 758
BseRI GAGGAG 2 cut(s) 174, 996
BseXI GCAGC 8 cut(s) 243, 392, 475, 638, 1060, 1324, 1327, 1417
BsgI GTGCAG 1 cut(s) 645
BshNI GGYRCC 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 1381
BsiSI CCGG 1 cut(s) 733
BslFI GGGAC 2 cut(s) 68, 897
BslI CCNNNNNNNGG 2 cut(s) 893, 1265
BsmAI GTCTC 2 cut(s) 1189, 1228
BsmFI GGGAC 2 cut(s) 68, 897
BsmI GAATGC 1 cut(s) 558
Bso31I GGTCTC 1 cut(s) 1189
Bsp143I GATC 6 cut(s) 22, 73, 78, 154, 520, 1378
BspACI CCGC 4 cut(s) 234, 408, 957, 1428
BspCNI CTCAG 4 cut(s) 71, 95, 788, 1185
BspDI ATCGAT 1 cut(s) 1381
BspLI GGNNCC 3 cut(s) 868, 885, 1152
BspPI GGATC 2 cut(s) 68, 1386
BspT107I GGYRCC 1 cut(s) 866
BspTNI GGTCTC 1 cut(s) 1189
BsrDI GCAATG 1 cut(s) 1212
BsrI ACTGG 1 cut(s) 758
BssECI CCNNGG 2 cut(s) 617, 731
BssMI GATC 6 cut(s) 22, 73, 78, 154, 520, 1378
BssNI GRCGYC 1 cut(s) 867
BssT1I CCWWGG 1 cut(s) 617
Bst2UI CCWGG 1 cut(s) 1445
Bst4CI ACNGT 2 cut(s) 949, 1222
Bst6I CTCTTC 1 cut(s) 1443
BstACI GRCGYC 1 cut(s) 867
BstBAI YACGTR 2 cut(s) 586, 1285
BstC8I GCNNGC 2 cut(s) 540, 624
BstDEI CTNAG 6 cut(s) 58, 82, 334, 339, 775, 1193
BstF5I GGATG 2 cut(s) 10, 1009
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 2 cut(s) 869, 1435
BstKTI GATC 6 cut(s) 25, 76, 81, 157, 523, 1381
BstMAI GTCTC 2 cut(s) 1189, 1228
BstMBI GATC 6 cut(s) 22, 73, 78, 154, 520, 1378
BstMWI GCNNNNNNNGC 4 cut(s) 335, 539, 1106, 1115
BstNI CCWGG 1 cut(s) 1445
BstNSI RCATGY 4 cut(s) 222, 451, 556, 1096
BstSCI CCNGG 2 cut(s) 731, 1443
BstSFI CTRYAG 1 cut(s) 165
BstSNI TACGTA 1 cut(s) 586
BstV1I GCAGC 8 cut(s) 243, 392, 475, 638, 1060, 1324, 1327, 1417
BstX2I RGATCY 1 cut(s) 73
BstXI CCANNNNNNTGG 1 cut(s) 1238
BstYI RGATCY 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 1381
BsuTUI ATCGAT 1 cut(s) 1381
BtgZI GCGATG 1 cut(s) 1226
BtsCI GGATG 2 cut(s) 10, 1009
Cac8I GCNNGC 2 cut(s) 540, 624
CaiI CAGNNNCTG 1 cut(s) 1340
CfoI GCGC 2 cut(s) 869, 1435
Cfr13I GGNCC 4 cut(s) 429, 884, 1151, 1350
ClaI ATCGAT 1 cut(s) 1381
Csp6I GTAC 2 cut(s) 216, 1247
CviAII CATG 9 cut(s) 93, 219, 295, 448, 518, 553, 1000, 1093, 1452
CviQI GTAC 2 cut(s) 216, 1247
DdeI CTNAG 6 cut(s) 58, 82, 334, 339, 775, 1193
DinI GGCGCC 1 cut(s) 868
DpnI GATC 6 cut(s) 24, 75, 80, 156, 522, 1380
DpnII GATC 6 cut(s) 22, 73, 78, 154, 520, 1378
Eam1104I CTCTTC 1 cut(s) 1443
EarI CTCTTC 1 cut(s) 1443
Eco105I TACGTA 1 cut(s) 586
Eco130I CCWWGG 1 cut(s) 617
Eco31I GGTCTC 1 cut(s) 1189
Eco47I GGWCC 4 cut(s) 429, 884, 1151, 1350
Eco57I CTGAAG 1 cut(s) 1259
EcoO109I RGGNCCY 1 cut(s) 1151
EcoRII CCWGG 1 cut(s) 1443
EcoT14I CCWWGG 1 cut(s) 617
EcoT22I ATGCAT 1 cut(s) 1102
EgeI GGCGCC 1 cut(s) 868
EheI GGCGCC 1 cut(s) 868
ErhI CCWWGG 1 cut(s) 617
FaeI CATG 9 cut(s) 96, 222, 298, 451, 521, 556, 1003, 1096, 1455
FalI AAGNNNNNCTT 4 cut(s) 101, 133, 633, 665
FaqI GGGAC 2 cut(s) 68, 897
FatI CATG 9 cut(s) 92, 218, 294, 447, 517, 552, 999, 1092, 1451
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 1 cut(s) 1217
Fnu4HI GCNGC 8 cut(s) 232, 406, 489, 627, 1074, 1338, 1341, 1431
FokI GGATG 2 cut(s) 17, 1016
Fsp4HI GCNGC 8 cut(s) 232, 406, 489, 627, 1074, 1338, 1341, 1431
FspBI CTAG 3 cut(s) 39, 671, 992
GlaI GCGC 2 cut(s) 868, 1434
GluI GCNGC 8 cut(s) 232, 406, 489, 627, 1074, 1338, 1341, 1431
GsuI CTGGAG 2 cut(s) 696, 1427
HaeII RGCGCY 1 cut(s) 870
HapII CCGG 1 cut(s) 733
HhaI GCGC 2 cut(s) 869, 1435
Hin1I GRCGYC 1 cut(s) 867
Hin1II CATG 9 cut(s) 96, 222, 298, 451, 521, 556, 1003, 1096, 1455
Hin6I GCGC 2 cut(s) 867, 1433
HinP1I GCGC 2 cut(s) 867, 1433
HincII GTYRAC 2 cut(s) 10, 136
HindII GTYRAC 2 cut(s) 10, 136
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 7 cut(s) 56, 62, 190, 388, 988, 1394, 1421
HpaII CCGG 1 cut(s) 733
HphI GGTGA 4 cut(s) 32, 476, 1136, 1248
Hpy166II GTNNAC 6 cut(s) 10, 99, 136, 1127, 1218, 1288
Hpy188I TCNGA 9 cut(s) 61, 78, 85, 368, 525, 564, 699, 1194, 1399
Hpy188III TCNNGA 7 cut(s) 385, 713, 978, 992, 1087, 1277, 1366
Hpy8I GTNNAC 6 cut(s) 10, 99, 136, 1127, 1218, 1288
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 4 cut(s) 11, 99, 623, 1260
HpyCH4III ACNGT 2 cut(s) 949, 1222
HpyCH4IV ACGT 4 cut(s) 585, 750, 1046, 1284
HpyCH4V TGCA 8 cut(s) 204, 558, 626, 694, 1100, 1205, 1343, 1409
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 539, 1106, 1115
HpyF3I CTNAG 6 cut(s) 58, 82, 334, 339, 775, 1193
HpySE526I ACGT 4 cut(s) 585, 750, 1046, 1284
Hsp92I GRCGYC 1 cut(s) 867
Hsp92II CATG 9 cut(s) 96, 222, 298, 451, 521, 556, 1003, 1096, 1455
HspAI GCGC 2 cut(s) 867, 1433
KasI GGCGCC 1 cut(s) 866
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 6 cut(s) 22, 73, 78, 154, 520, 1378
LmnI GCTCC 3 cut(s) 161, 715, 1055
Lsp1109I GCAGC 8 cut(s) 243, 392, 475, 638, 1060, 1324, 1327, 1417
LweI GCATC 4 cut(s) 479, 703, 994, 1396
MaeI CTAG 3 cut(s) 39, 671, 992
MaeII ACGT 4 cut(s) 585, 750, 1046, 1284
MaeIII GTNAC 4 cut(s) 823, 943, 1300, 1411
MalI GATC 6 cut(s) 24, 75, 80, 156, 522, 1380
MboI GATC 6 cut(s) 22, 73, 78, 154, 520, 1378
MboII GAAGA 4 cut(s) 281, 1265, 1403, 1430
MfeI CAATTG 1 cut(s) 1291
MflI RGATCY 1 cut(s) 73
MluCI AATT 9 cut(s) 42, 145, 262, 797, 960, 1011, 1159, 1291, 1344
Mly113I GGCGCC 1 cut(s) 867
MlyI GAGTC 3 cut(s) 50, 199, 382
MmeI TCCRAC 2 cut(s) 587, 1246
MnlI CCTC 8 cut(s) 45, 152, 325, 460, 932, 974, 1264, 1446
Mph1103I ATGCAT 1 cut(s) 1102
MseI TTAA 4 cut(s) 140, 348, 746, 1026
MspA1I CMGCKG 4 cut(s) 234, 779, 1337, 1430
MspI CCGG 1 cut(s) 733
MspR9I CCNGG 2 cut(s) 733, 1445
MunI CAATTG 1 cut(s) 1291
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 1 cut(s) 1445
MwoI GCNNNNNNNGC 4 cut(s) 335, 539, 1106, 1115
NarI GGCGCC 1 cut(s) 867
NciI CCSGG 1 cut(s) 733
NdeII GATC 6 cut(s) 22, 73, 78, 154, 520, 1378
NlaIII CATG 9 cut(s) 96, 222, 298, 451, 521, 556, 1003, 1096, 1455
NlaIV GGNNCC 3 cut(s) 868, 885, 1152
NmuCI GTSAC 2 cut(s) 943, 1300
NsiI ATGCAT 1 cut(s) 1102
NspI RCATGY 4 cut(s) 222, 451, 556, 1096
PciI ACATGT 2 cut(s) 218, 552
PcsI WCGNNNNNNNCGW 1 cut(s) 1395
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 4 cut(s) 62, 988, 1394, 1421
PflMI CCANNNNNTGG 2 cut(s) 893, 1265
PfoI TCCNGGA 1 cut(s) 1443
PkrI GCNGC 8 cut(s) 233, 407, 490, 628, 1075, 1339, 1342, 1432
PleI GAGTC 3 cut(s) 50, 198, 382
PluTI GGCGCC 1 cut(s) 870
PpsI GAGTC 3 cut(s) 50, 198, 382
Ppu21I YACGTR 2 cut(s) 586, 1285
PpuMI RGGWCCY 1 cut(s) 1151
PscI ACATGT 2 cut(s) 218, 552
PsiI TTATAA 1 cut(s) 900
Psp1406I AACGTT 2 cut(s) 750, 1046
Psp5II RGGWCCY 1 cut(s) 1151
Psp6I CCWGG 1 cut(s) 1443
PspGI CCWGG 1 cut(s) 1443
PspN4I GGNNCC 3 cut(s) 868, 885, 1152
PspPI GGNCC 4 cut(s) 429, 884, 1151, 1350
PspPPI RGGWCCY 1 cut(s) 1151
PstNI CAGNNNCTG 1 cut(s) 1340
PsuI RGATCY 1 cut(s) 73
PvuII CAGCTG 2 cut(s) 779, 1337
RsaI GTAC 2 cut(s) 217, 1248
RsaNI GTAC 2 cut(s) 216, 1247
SaqAI TTAA 4 cut(s) 140, 348, 746, 1026
SatI GCNGC 8 cut(s) 232, 406, 489, 627, 1074, 1338, 1341, 1431
Sau3AI GATC 6 cut(s) 22, 73, 78, 154, 520, 1378
Sau96I GGNCC 4 cut(s) 429, 884, 1151, 1350
SchI GAGTC 3 cut(s) 50, 199, 382
ScrFI CCNGG 2 cut(s) 733, 1445
SfaNI GCATC 4 cut(s) 479, 703, 994, 1396
SfcI CTRYAG 1 cut(s) 165
SfoI GGCGCC 1 cut(s) 868
SinI GGWCC 4 cut(s) 429, 884, 1151, 1350
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
SnaBI TACGTA 1 cut(s) 586
Sse9I AATT 9 cut(s) 42, 145, 262, 797, 960, 1011, 1159, 1291, 1344
SsiI CCGC 4 cut(s) 234, 408, 957, 1428
SspDI GGCGCC 1 cut(s) 866
SspI AATATT 1 cut(s) 1062
SspMI CTAG 3 cut(s) 39, 671, 992
StyD4I CCNGG 2 cut(s) 731, 1443
StyI CCWWGG 1 cut(s) 617
TaaI ACNGT 2 cut(s) 949, 1222
TaiI ACGT 4 cut(s) 588, 753, 1049, 1287
TaqI TCGA 4 cut(s) 157, 209, 501, 1381
TasI AATT 9 cut(s) 42, 145, 262, 797, 960, 1011, 1159, 1291, 1344
TfiI GAWTC 4 cut(s) 62, 988, 1394, 1421
Tru1I TTAA 4 cut(s) 140, 348, 746, 1026
Tru9I TTAA 4 cut(s) 140, 348, 746, 1026
TseFI GTSAC 2 cut(s) 943, 1300
TseI GCWGC 8 cut(s) 231, 405, 488, 626, 1073, 1337, 1340, 1430
Tsp45I GTSAC 2 cut(s) 943, 1300
TspDTI ATGAA 7 cut(s) 81, 213, 283, 487, 800, 864, 1153
TspGWI ACGGA 2 cut(s) 27, 342
Van91I CCANNNNNTGG 2 cut(s) 893, 1265
VpaK11BI GGWCC 4 cut(s) 429, 884, 1151, 1350
XapI RAATTY 1 cut(s) 1159
XbaI TCTAGA 1 cut(s) 991
XceI RCATGY 4 cut(s) 222, 451, 556, 1096
XmiI GTMKAC 1 cut(s) 1217
XspI CTAG 3 cut(s) 39, 671, 992
Zsp2I ATGCAT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.