Rh6DG156200

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001054
Physical Location & Seq
Reverse (-)
23445296 .. 23454688
9393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG156200.1

Sequence Viewer

Length: 1278 bp
ATGGCCAATCCTCAACATGAAGAAGAAGAATCAAAGTCAGTCGTCTCCATTGCCTCAACCTCATTAAGGAAATCAAGGAAATCAAAGCGTCCTTCCTGGTTCACACCCAAGAGGTTACTTCTCATATTCTGTGTGATCAACTTGGTAAATTATGTGGACCGAGGAGCAATATCAAGCAACGGCGTGAATGGGAACCTTCCAATTTGCAGGAATGGGAAATGCAAACCTGCTACCGGCATACAGGGGCATTTTTTACTGAACAATTTTTCAGTTGGATGTCTATCGTCAGCTTTTATGGTTGGACTTCTGGTGGCTTCTCCAATTTTTGCAGCACTAGCAAAGAGTGTAAATCCATTTAAGCTCATTGGAGTTGGATTGTCAATTTGGACTTTTGCTGTAACTGGTTGTGGTTTTTCATTCAACTTCTTGTCTATCTTAATCTGCCGCATGCTAGTTGGTGTTGGTGAGGCTTCGTTTATTAGTCTTGCAGCTCCATTCATTGATGATAATGCCCCAGCTGCTCAGAAAACTCTATGGCTTTCAACATTCTACATGTGCATACCATCTGGATATGCACTTGGTTATGTTTACGGAGGCTTGGTGGGAACTACTCTCAGTTGGCGTGCTGCATTTTGGGGGGAGGGAATTCTGATGCTTCCATTTGCTATTCTAGGATTTGTTATGAAGCCTTTGCGGATGAAAGGTTTTAAGCATGATGAATCAAATGAAGCATTGACTGTGCAGGAGACGGCTGTCTCAGAAGTTCAAGGTGCTTTGAACCCGAAGGATAGGGCAGTTTCAATGAAGGAAGATGTAACAAATTCTACCATACACAAACCTTCCAAGCCAAAAATTGCAACAAAAATAAAGAATCATCTTTCAAGGTTTATGAAAGATATGAAGGATCTTTTGGTCGATAAGGTTTATGTTGTTAATGTTCTTGGTTATGTAGCATACAACTTTGTAATTGGTTCGTACTCATATTGGGGGCCTAAGGCTGGTTATAATATCTATCATATGACTAATGCAGATATGATTTTTGGAGGTATTACAATTGTGTGCGGAATATTGGGAACACTAGCAGGAGGGTTTCTTCTGGATTTCATGTCTAACACTATGTCCAATGCTTTCAAGCTTCTTTCAACAGCAACGCTTTTTGGGGCAATATTATGCTTTGGTGCCTTTTGCTTGAAGAACATGTATGGTTTCCTAGCTCTTTTTGCAGTTGGGCAACTACTTCTTTTTGCAACTCAGGTACAAATCCAATCAAATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

425

Amino Acids

46.25

Weight (kDa)

9.39

Isoelectric Point (pI)

23.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 79 - 417 6.5e-30 Major Facilitator Superfamily
OATP PF03137 144 - 396 3.7e-20 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 1178
AciI CCGC 3 cut(s) 445, 694, 1062
AclWI GGATC 1 cut(s) 912
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 645, 820
AfaI GTAC 2 cut(s) 977, 1257
AfiI CCNNNNNNNGG 3 cut(s) 66, 233, 998
AflIII ACRYGT 2 cut(s) 552, 1197
AgsI TTSAA 9 cut(s) 421, 543, 767, 778, 801, 882, 1132, 1143, 1192
AjnI CCWGG 1 cut(s) 95
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
AluBI AGCT 6 cut(s) 290, 361, 491, 518, 1135, 1214
AluI AGCT 6 cut(s) 290, 361, 491, 518, 1135, 1214
Alw26I GTCTC 3 cut(s) 49, 740, 760
AlwI GGATC 1 cut(s) 912
AoxI GGCC 2 cut(s) 3, 989
ApeKI GCWGC 4 cut(s) 329, 488, 518, 626
ApoI RAATTY 2 cut(s) 645, 820
AspS9I GGNCC 2 cut(s) 157, 989
AsuHPI GGTGA 1 cut(s) 476
AvaII GGWCC 1 cut(s) 157
AxyI CCTNAGG 1 cut(s) 993
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 1178
BbvI GCAGC 4 cut(s) 341, 500, 505, 613
BccI CCATC 1 cut(s) 571
BceAI ACGGC 2 cut(s) 196, 765
BciT130I CCWGG 1 cut(s) 97
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 49, 740, 760
BfaI CTAG 5 cut(s) 335, 452, 671, 1079, 1211
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 5 cut(s) 330, 445, 489, 519, 627
BlsI GCNGC 5 cut(s) 331, 446, 490, 520, 628
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 2 cut(s) 157, 989
BmiI GGNNCC 3 cut(s) 194, 990, 1180
BmrFI CCNGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 642
BoxI GACNNNNGTC 1 cut(s) 752
BsaBI GATNNNNATC 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 233, 998
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 406
Bse21I CCTNAGG 1 cut(s) 993
Bse3DI GCAATG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 280
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 281, 702
BseJI GATNNNNATC 1 cut(s) 280
BseLI CCNNNNNNNGG 3 cut(s) 66, 233, 998
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 4 cut(s) 536, 628, 771, 1265
BseNI ACTGG 1 cut(s) 406
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 4 cut(s) 341, 500, 505, 613
BseYI CCCAGC 1 cut(s) 514
BsgI GTGCAG 1 cut(s) 761
BshFI GGCC 2 cut(s) 5, 991
BshNI GGYRCC 1 cut(s) 1178
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 3 cut(s) 66, 233, 998
BsmAI GTCTC 3 cut(s) 49, 740, 760
BsmBI CGTCTC 2 cut(s) 49, 740
BsnI GGCC 2 cut(s) 5, 991
Bsp143I GATC 2 cut(s) 135, 904
BspACI CCGC 3 cut(s) 445, 694, 1062
BspANI GGCC 2 cut(s) 5, 991
BspCNI CTCAG 4 cut(s) 535, 627, 770, 1264
BspLI GGNNCC 3 cut(s) 194, 990, 1180
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 1 cut(s) 912
BspT107I GGYRCC 1 cut(s) 1178
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 2 cut(s) 135, 904
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 739
BstC8I GCNNGC 2 cut(s) 449, 624
BstDEI CTNAG 5 cut(s) 522, 614, 757, 993, 1251
BstENI CCTNNNNNAGG 1 cut(s) 64
BstF5I GGATG 2 cut(s) 281, 702
BstKTI GATC 2 cut(s) 138, 907
BstMAI GTCTC 3 cut(s) 49, 740, 760
BstMBI GATC 2 cut(s) 135, 904
BstMWI GCNNNNNNNGC 3 cut(s) 335, 518, 1220
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 3 cut(s) 451, 556, 1201
BstPAI GACNNNNGTC 1 cut(s) 752
BstSCI CCNGG 1 cut(s) 95
BstV1I GCAGC 4 cut(s) 341, 500, 505, 613
BstX2I RGATCY 1 cut(s) 904
BstYI RGATCY 1 cut(s) 904
Bsu36I CCTNAGG 1 cut(s) 993
BsuRI GGCC 2 cut(s) 5, 991
BtsCI GGATG 2 cut(s) 281, 702
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 449, 624
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 157, 989
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 976, 1256
CviAII CATG 6 cut(s) 17, 448, 553, 713, 1105, 1198
CviQI GTAC 2 cut(s) 976, 1256
DdeI CTNAG 5 cut(s) 522, 614, 757, 993, 1251
DpnI GATC 2 cut(s) 137, 906
DpnII GATC 2 cut(s) 135, 904
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 157
Eco81I CCTNAGG 1 cut(s) 993
EcoNI CCTNNNNNAGG 1 cut(s) 64
EcoO109I RGGNCCY 1 cut(s) 989
EcoRI GAATTC 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 95
Esp3I CGTCTC 2 cut(s) 49, 740
FaeI CATG 6 cut(s) 20, 451, 556, 716, 1108, 1201
FatI CATG 6 cut(s) 16, 447, 552, 712, 1104, 1197
FauNDI CATATG 1 cut(s) 1017
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 330, 445, 489, 519, 627
FokI GGATG 2 cut(s) 288, 709
Fsp4HI GCNGC 5 cut(s) 330, 445, 489, 519, 627
FspBI CTAG 5 cut(s) 335, 452, 671, 1079, 1211
GluI GCNGC 5 cut(s) 330, 445, 489, 519, 627
GsaI CCCAGC 1 cut(s) 518
HaeIII GGCC 2 cut(s) 5, 991
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 77
Hin1II CATG 6 cut(s) 20, 451, 556, 716, 1108, 1201
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 3 cut(s) 29, 719, 871
HpaII CCGG 1 cut(s) 234
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 3 cut(s) 102, 157, 589
Hpy188I TCNGA 3 cut(s) 525, 651, 760
Hpy188III TCNNGA 2 cut(s) 567, 1097
Hpy8I GTNNAC 3 cut(s) 102, 157, 589
HpyAV CCTTC 6 cut(s) 102, 206, 778, 799, 849, 895
HpyCH4III ACNGT 1 cut(s) 739
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 518, 1220
HpyF3I CTNAG 5 cut(s) 522, 614, 757, 993, 1251
Hsp92II CATG 6 cut(s) 20, 451, 556, 716, 1108, 1201
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 2 cut(s) 135, 904
LmnI GCTCC 2 cut(s) 164, 496
Lsp1109I GCAGC 4 cut(s) 341, 500, 505, 613
LweI GCATC 1 cut(s) 642
MaeI CTAG 5 cut(s) 335, 452, 671, 1079, 1211
MaeIII GTNAC 3 cut(s) 114, 397, 814
MalI GATC 2 cut(s) 137, 906
MboI GATC 2 cut(s) 135, 904
MboII GAAGA 6 cut(s) 32, 35, 38, 821, 1085, 1204
MfeI CAATTG 1 cut(s) 1053
MflI RGATCY 1 cut(s) 904
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 253, 280, 352
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 65, 357, 437, 708, 933
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 97
MunI CAATTG 1 cut(s) 1053
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 3 cut(s) 335, 518, 1220
NdeI CATATG 1 cut(s) 1017
NdeII GATC 2 cut(s) 135, 904
NlaIII CATG 6 cut(s) 20, 451, 556, 716, 1108, 1201
NlaIV GGNNCC 3 cut(s) 194, 990, 1180
NspI RCATGY 3 cut(s) 451, 556, 1201
PaeI GCATGC 1 cut(s) 451
PciI ACATGT 2 cut(s) 552, 1197
PfeI GAWTC 3 cut(s) 29, 719, 871
PkrI GCNGC 5 cut(s) 331, 446, 490, 520, 628
PscI ACATGT 2 cut(s) 552, 1197
PshAI GACNNNNGTC 1 cut(s) 752
PsiI TTATAA 1 cut(s) 1005
Psp6I CCWGG 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 514
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 3 cut(s) 194, 990, 1180
PspPI GGNCC 2 cut(s) 157, 989
PsuI RGATCY 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 2 cut(s) 977, 1257
RsaNI GTAC 2 cut(s) 976, 1256
SaqAI TTAA 5 cut(s) 65, 357, 437, 708, 933
SatI GCNGC 5 cut(s) 330, 445, 489, 519, 627
Sau3AI GATC 2 cut(s) 135, 904
Sau96I GGNCC 2 cut(s) 157, 989
ScrFI CCNGG 1 cut(s) 97
SfaNI GCATC 1 cut(s) 642
SinI GGWCC 1 cut(s) 157
SphI GCATGC 1 cut(s) 451
SsiI CCGC 3 cut(s) 445, 694, 1062
SspI AATATT 2 cut(s) 1068, 1167
SspMI CTAG 5 cut(s) 335, 452, 671, 1079, 1211
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 739
TaqI TCGA 1 cut(s) 915
TaqII GACCGA 1 cut(s) 174
TauI GCSGC 1 cut(s) 447
TfiI GAWTC 3 cut(s) 29, 719, 871
Tru1I TTAA 5 cut(s) 65, 357, 437, 708, 933
Tru9I TTAA 5 cut(s) 65, 357, 437, 708, 933
TseI GCWGC 4 cut(s) 329, 488, 518, 626
TspGWI ACGGA 1 cut(s) 606
VpaK11BI GGWCC 1 cut(s) 157
XagI CCTNNNNNAGG 1 cut(s) 64
XapI RAATTY 2 cut(s) 645, 820
XceI RCATGY 3 cut(s) 451, 556, 1201
XspI CTAG 5 cut(s) 335, 452, 671, 1079, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.