Rh6BG171100

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001054
Physical Location & Seq
Reverse (-)
28222379 .. 28235422
13044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG171100.1

Sequence Viewer

Length: 1623 bp
ATGGCCAATCCTCAACATGAAGAAGAAGAATCAAAGTCAGTCGTCTCCATTGCCTCAACCTCATTAAGGAAATCAAGGAAATCAAAGCGTCCTTCCTGGTTCACCCCCAAGAGGTTACTTCTCATATTCTGTGTGATCAACTTGGTAAATTATGTGGACCGAGGAGCAATATCAAGCAACGGCGTGAATGGGAACCTTCCAATTTGCAGGAATGGTGAATGCAAACCTGCTACCGGCATACAGGGGCATTTTTTACTGAACAATTTTTCAGTTGGATGTCTATCGTCAGCTTTTATGGTTGGACTTCTGGTGGCTTCTCCAATTTTTGCAGCACTAGCAAAGAGTGTAAATCCATTTAAGCTCATTGGAGTTGGATTGTCAATTTGGACTTTTGCTGTAACTGGTTGTGGTTTTTCATTCAACTTCTTGTCTATCTTAATCTGCCGCATGCTAGTTGGTGTTGGTGAGGCTTCGTTTATTAGTCTTGCAGCTCCGTTCATTGATGATAATGCCCCAGCTGCTCAGAAAACTCTATGGCTTTCAACATTCTACATGTGCATACCATCTGGATATGCACTTGGTTATGTTTACGGAGGCTTGGTGGGAACTACTCTCAGTTGGCGTGCTGCATTTTGGGGGGAGGGAATTCTGATGCTTCCATTTGCTATTCTAGGATTTGTTATGAAGCCTTTGCGGATGAAAGGTTTTAAGCATGATGAATCAATTGAAGCATTGACTGTGCAGGAGACGGCTGTCTCAGAAGTTCAAGGTGCTTTGGACCCCAAGGATAGGGCAGTTTCAATGAAGGAAGATGTAACAAATTCTACCATACACAGACCTTCCAAGCCAAAAATTGCAACAAAAATAAAGAATCATCTTTCAAGGTTTATGAAAGATATGAAGGATCTTTTGGTCGATAAGGTTTATGTTGTTAATGTTCTTGGTTATGTAGCATACAACTTTGTAATTGGTTCGTACTCATATTGGGGGCCTAAGGCTGGTTATAATATCTATCATATGACTAATGCAGATATGATTTTTGGAGGTATTACAATTGTGTGTGGAATAGTGGGAACACTAGCAGGAGGCTTTCTTCTGGATTTCATGTCTAACACTATGTCCAATGCTTTCAAGCTTCTTTCAACAGCAACGCTTTTTGGGGCAATATTATGCTTTGGTGCCTTTTGCTTAAAGAACATGTATGGTTTTCTAGCTCTTTTTGCAGTTGGGCAACTACTTCTCTTTGCAACTCAGGGTCCTGTAAATTTTGTTACTCTTCATTGTGTTAAACCTAGTTTGAGGCCACTATCTATGGCCATTTGTACTGTTGCAATTCACATATTTGGAGATGTGCCTTCCTCACCTCTTGTTGGAGTCGTCCAGGATCACATCAAGAATTGGAGGTCAACTGCTCTTATTCTAACATCCGTTTTGATTCCTGCAGCGGCCATATGGTTTACAGCAATTTTCATGGACAGCGTGGATAGATATGACGAAGAAAGTGATGATGAAGAAAGTGATGATGAGATAGAAACAACTGAAATGCCGTCGCTTGAAGAGAAGAAGACGGAGAAAACAACTGAATCTGCTGCTCAACCTGTCACATATTCAACTGTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

540

Amino Acids

58.87

Weight (kDa)

7.08

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 79 - 445 1.8e-29 Major Facilitator Superfamily
OATP PF03137 143 - 396 3.8e-19 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 395 - 463 8.2e-07 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 1178
AciI CCGC 3 cut(s) 445, 694, 1445
AclWI GGATC 2 cut(s) 912, 1392
AcoI YGGCCR 3 cut(s) 3, 1314, 1446
AcsI RAATTY 3 cut(s) 645, 820, 1264
AfaI GTAC 2 cut(s) 977, 1324
AfiI CCNNNNNNNGG 6 cut(s) 66, 111, 233, 789, 998, 1370
AflIII ACRYGT 2 cut(s) 552, 1197
AjnI CCWGG 2 cut(s) 95, 1380
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
AluBI AGCT 6 cut(s) 290, 361, 491, 518, 1135, 1214
AluI AGCT 6 cut(s) 290, 361, 491, 518, 1135, 1214
Alw26I GTCTC 3 cut(s) 49, 740, 760
AlwI GGATC 2 cut(s) 912, 1392
AoxI GGCC 5 cut(s) 3, 989, 1301, 1314, 1446
ApeKI GCWGC 6 cut(s) 329, 488, 518, 626, 1442, 1589
ApoI RAATTY 3 cut(s) 645, 820, 1264
AspS9I GGNCC 4 cut(s) 157, 778, 989, 1256
AsuHPI GGTGA 4 cut(s) 94, 227, 476, 1353
AvaII GGWCC 3 cut(s) 157, 778, 1256
AxyI CCTNAGG 1 cut(s) 993
BalI TGGCCA 2 cut(s) 5, 1316
BanI GGYRCC 1 cut(s) 1178
BbsI GAAGAC 1 cut(s) 1571
BbvI GCAGC 6 cut(s) 341, 500, 505, 613, 1454, 1576
BccI CCATC 1 cut(s) 571
BceAI ACGGC 3 cut(s) 196, 765, 1531
BciT130I CCWGG 2 cut(s) 97, 1382
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 49, 740, 760
BfaI CTAG 7 cut(s) 335, 452, 671, 1079, 1211, 1293, 1621
BfmI CTRYAG 1 cut(s) 1440
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 8 cut(s) 330, 445, 489, 519, 627, 1443, 1446, 1590
BlsI GCNGC 8 cut(s) 331, 446, 490, 520, 628, 1444, 1447, 1591
Bme1390I CCNGG 2 cut(s) 97, 1382
Bme18I GGWCC 3 cut(s) 157, 778, 1256
BmgT120I GGNCC 4 cut(s) 157, 778, 989, 1256
BmiI GGNNCC 5 cut(s) 194, 780, 990, 1180, 1257
BmrFI CCNGG 2 cut(s) 97, 1382
BmsI GCATC 1 cut(s) 642
BoxI GACNNNNGTC 1 cut(s) 752
BpiI GAAGAC 1 cut(s) 1571
BsaBI GATNNNNATC 1 cut(s) 280
BsaJI CCNNGG 2 cut(s) 160, 783
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 111, 233, 789, 998, 1370
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 406
Bse21I CCTNAGG 1 cut(s) 993
Bse3DI GCAATG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 280
BseBI CCWGG 2 cut(s) 97, 1382
BseDI CCNNGG 2 cut(s) 160, 783
BseGI GGATG 3 cut(s) 281, 702, 1424
BseJI GATNNNNATC 1 cut(s) 280
BseLI CCNNNNNNNGG 6 cut(s) 66, 111, 233, 789, 998, 1370
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 4 cut(s) 536, 628, 771, 1265
BseNI ACTGG 1 cut(s) 406
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 6 cut(s) 341, 500, 505, 613, 1454, 1576
BseYI CCCAGC 1 cut(s) 514
BsgI GTGCAG 1 cut(s) 761
BshFI GGCC 5 cut(s) 5, 991, 1303, 1316, 1448
BshNI GGYRCC 1 cut(s) 1178
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 6 cut(s) 66, 111, 233, 789, 998, 1370
BsmAI GTCTC 3 cut(s) 49, 740, 760
BsmBI CGTCTC 2 cut(s) 49, 740
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 5 cut(s) 5, 991, 1303, 1316, 1448
Bsp143I GATC 3 cut(s) 135, 904, 1384
BspACI CCGC 3 cut(s) 445, 694, 1445
BspANI GGCC 5 cut(s) 5, 991, 1303, 1316, 1448
BspCNI CTCAG 4 cut(s) 535, 627, 770, 1264
BspLI GGNNCC 5 cut(s) 194, 780, 990, 1180, 1257
BspMAI CTGCAG 1 cut(s) 1444
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 2 cut(s) 912, 1392
BspT107I GGYRCC 1 cut(s) 1178
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 160, 783
BssMI GATC 3 cut(s) 135, 904, 1384
BssT1I CCWWGG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 97, 1382
Bst4CI ACNGT 3 cut(s) 739, 1327, 1615
Bst6I CTCTTC 2 cut(s) 1281, 1551
BstC8I GCNNGC 2 cut(s) 449, 624
BstDEI CTNAG 5 cut(s) 522, 614, 757, 993, 1251
BstENI CCTNNNNNAGG 1 cut(s) 64
BstF5I GGATG 3 cut(s) 281, 702, 1424
BstKTI GATC 3 cut(s) 138, 907, 1387
BstMAI GTCTC 3 cut(s) 49, 740, 760
BstMBI GATC 3 cut(s) 135, 904, 1384
BstMWI GCNNNNNNNGC 3 cut(s) 335, 518, 1220
BstNI CCWGG 2 cut(s) 97, 1382
BstNSI RCATGY 3 cut(s) 451, 556, 1201
BstPAI GACNNNNGTC 1 cut(s) 752
BstSCI CCNGG 2 cut(s) 95, 1380
BstSFI CTRYAG 1 cut(s) 1440
BstV1I GCAGC 6 cut(s) 341, 500, 505, 613, 1454, 1576
BstV2I GAAGAC 1 cut(s) 1571
BstX2I RGATCY 1 cut(s) 904
BstYI RGATCY 1 cut(s) 904
Bsu36I CCTNAGG 1 cut(s) 993
BsuRI GGCC 5 cut(s) 5, 991, 1303, 1316, 1448
BtsCI GGATG 3 cut(s) 281, 702, 1424
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 449, 624
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 4 cut(s) 157, 778, 989, 1256
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 976, 1323
CviAII CATG 7 cut(s) 17, 448, 553, 713, 1105, 1198, 1471
CviQI GTAC 2 cut(s) 976, 1323
DdeI CTNAG 5 cut(s) 522, 614, 757, 993, 1251
DpnI GATC 3 cut(s) 137, 906, 1386
DpnII GATC 3 cut(s) 135, 904, 1384
EaeI YGGCCR 3 cut(s) 3, 1314, 1446
Eam1104I CTCTTC 2 cut(s) 1281, 1551
EarI CTCTTC 2 cut(s) 1281, 1551
Eco130I CCWWGG 1 cut(s) 783
Eco47I GGWCC 3 cut(s) 157, 778, 1256
Eco81I CCTNAGG 1 cut(s) 993
EcoNI CCTNNNNNAGG 1 cut(s) 64
EcoO109I RGGNCCY 2 cut(s) 989, 1256
EcoRI GAATTC 1 cut(s) 645
EcoRII CCWGG 2 cut(s) 95, 1380
EcoT14I CCWWGG 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 783
Esp3I CGTCTC 2 cut(s) 49, 740
FaeI CATG 7 cut(s) 20, 451, 556, 716, 1108, 1201, 1474
FatI CATG 7 cut(s) 16, 447, 552, 712, 1104, 1197, 1470
FauNDI CATATG 2 cut(s) 1017, 1451
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 8 cut(s) 330, 445, 489, 519, 627, 1443, 1446, 1590
FokI GGATG 3 cut(s) 288, 709, 1411
Fsp4HI GCNGC 8 cut(s) 330, 445, 489, 519, 627, 1443, 1446, 1590
FspBI CTAG 7 cut(s) 335, 452, 671, 1079, 1211, 1293, 1621
GluI GCNGC 8 cut(s) 330, 445, 489, 519, 627, 1443, 1446, 1590
GsaI CCCAGC 1 cut(s) 518
HaeIII GGCC 5 cut(s) 5, 991, 1303, 1316, 1448
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 77
Hin1II CATG 7 cut(s) 20, 451, 556, 716, 1108, 1201, 1474
HincII GTYRAC 1 cut(s) 1407
HindII GTYRAC 1 cut(s) 1407
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 6 cut(s) 29, 719, 871, 1374, 1435, 1583
HpaII CCGG 1 cut(s) 234
HphI GGTGA 4 cut(s) 94, 227, 476, 1353
Hpy166II GTNNAC 5 cut(s) 102, 157, 589, 1407, 1458
Hpy188I TCNGA 3 cut(s) 525, 651, 760
Hpy188III TCNNGA 3 cut(s) 567, 1097, 1393
Hpy8I GTNNAC 5 cut(s) 102, 157, 589, 1407, 1458
Hpy99I CGWCG 1 cut(s) 1552
HpyAV CCTTC 6 cut(s) 102, 206, 799, 849, 895, 1365
HpyCH4III ACNGT 3 cut(s) 739, 1327, 1615
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 518, 1220
HpyF3I CTNAG 5 cut(s) 522, 614, 757, 993, 1251
Hsp92II CATG 7 cut(s) 20, 451, 556, 716, 1108, 1201, 1474
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 3 cut(s) 135, 904, 1384
LmnI GCTCC 2 cut(s) 164, 496
Lsp1109I GCAGC 6 cut(s) 341, 500, 505, 613, 1454, 1576
LweI GCATC 1 cut(s) 642
MaeI CTAG 7 cut(s) 335, 452, 671, 1079, 1211, 1293, 1621
MaeIII GTNAC 5 cut(s) 114, 397, 814, 1270, 1600
MalI GATC 3 cut(s) 137, 906, 1386
MboI GATC 3 cut(s) 135, 904, 1384
MfeI CAATTG 2 cut(s) 723, 1053
MflI RGATCY 1 cut(s) 904
MlsI TGGCCA 2 cut(s) 5, 1316
MluNI TGGCCA 2 cut(s) 5, 1316
MlyI GAGTC 1 cut(s) 1383
MmeI TCCRAC 4 cut(s) 253, 280, 352, 1351
Mox20I TGGCCA 2 cut(s) 5, 1316
MscI TGGCCA 2 cut(s) 5, 1316
MseI TTAA 7 cut(s) 65, 357, 437, 708, 933, 1190, 1287
Msp20I TGGCCA 2 cut(s) 5, 1316
MspA1I CMGCKG 2 cut(s) 518, 1445
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 2 cut(s) 97, 1382
MunI CAATTG 2 cut(s) 723, 1053
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 2 cut(s) 97, 1382
MwoI GCNNNNNNNGC 3 cut(s) 335, 518, 1220
NdeI CATATG 2 cut(s) 1017, 1451
NdeII GATC 3 cut(s) 135, 904, 1384
NlaIII CATG 7 cut(s) 20, 451, 556, 716, 1108, 1201, 1474
NlaIV GGNNCC 5 cut(s) 194, 780, 990, 1180, 1257
NmuCI GTSAC 1 cut(s) 1600
NspI RCATGY 3 cut(s) 451, 556, 1201
PaeI GCATGC 1 cut(s) 451
PciI ACATGT 2 cut(s) 552, 1197
PctI GAATGC 1 cut(s) 224
PfeI GAWTC 5 cut(s) 29, 719, 871, 1435, 1583
PfoI TCCNGGA 1 cut(s) 1380
PkrI GCNGC 8 cut(s) 331, 446, 490, 520, 628, 1444, 1447, 1591
PleI GAGTC 1 cut(s) 1382
PpsI GAGTC 1 cut(s) 1382
PpuMI RGGWCCY 1 cut(s) 1256
PscI ACATGT 2 cut(s) 552, 1197
PshAI GACNNNNGTC 1 cut(s) 752
PsiI TTATAA 1 cut(s) 1005
Psp5II RGGWCCY 1 cut(s) 1256
Psp6I CCWGG 2 cut(s) 95, 1380
PspFI CCCAGC 1 cut(s) 514
PspGI CCWGG 2 cut(s) 95, 1380
PspN4I GGNNCC 5 cut(s) 194, 780, 990, 1180, 1257
PspPI GGNCC 4 cut(s) 157, 778, 989, 1256
PspPPI RGGWCCY 1 cut(s) 1256
PstI CTGCAG 1 cut(s) 1444
PsuI RGATCY 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 2 cut(s) 977, 1324
RsaNI GTAC 2 cut(s) 976, 1323
SaqAI TTAA 7 cut(s) 65, 357, 437, 708, 933, 1190, 1287
SatI GCNGC 8 cut(s) 330, 445, 489, 519, 627, 1443, 1446, 1590
Sau3AI GATC 3 cut(s) 135, 904, 1384
Sau96I GGNCC 4 cut(s) 157, 778, 989, 1256
SchI GAGTC 1 cut(s) 1383
ScrFI CCNGG 2 cut(s) 97, 1382
SfaNI GCATC 1 cut(s) 642
SfcI CTRYAG 1 cut(s) 1440
SinI GGWCC 3 cut(s) 157, 778, 1256
SphI GCATGC 1 cut(s) 451
SsiI CCGC 3 cut(s) 445, 694, 1445
SspI AATATT 1 cut(s) 1167
SspMI CTAG 7 cut(s) 335, 452, 671, 1079, 1211, 1293, 1621
StyD4I CCNGG 2 cut(s) 95, 1380
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 3 cut(s) 739, 1327, 1615
TaqI TCGA 1 cut(s) 915
TaqII GACCGA 1 cut(s) 174
TatI WGTACW 1 cut(s) 1322
TauI GCSGC 2 cut(s) 447, 1448
TfiI GAWTC 5 cut(s) 29, 719, 871, 1435, 1583
Tru1I TTAA 7 cut(s) 65, 357, 437, 708, 933, 1190, 1287
Tru9I TTAA 7 cut(s) 65, 357, 437, 708, 933, 1190, 1287
TseFI GTSAC 1 cut(s) 1600
TseI GCWGC 6 cut(s) 329, 488, 518, 626, 1442, 1589
Tsp45I GTSAC 1 cut(s) 1600
TspGWI ACGGA 4 cut(s) 483, 606, 1417, 1583
VpaK11BI GGWCC 3 cut(s) 157, 778, 1256
XagI CCTNNNNNAGG 1 cut(s) 64
XapI RAATTY 3 cut(s) 645, 820, 1264
XceI RCATGY 3 cut(s) 451, 556, 1201
XspI CTAG 7 cut(s) 335, 452, 671, 1079, 1211, 1293, 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.