pycom12g18820

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001054
Physical Location & Seq
Forward (+)
20894822 .. 20896396
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18820.1

Sequence Viewer

Length: 1575 bp
ATGGCTCAAAAGGAAGCAAAGGCTTCTGAAGAAGCCAAGCCCCCGTCCCCGGCGGAGACATCCATGTTTATTGCTTCAACCGCAGCTCCGGCAGCTCCTTCATGGTTCACGCCCAAGAGGCTACTCGTTATCTTTTGCTTGATTAACTTGTTAAACTATGTGGACCGAGGAACAATAGCAAGCAATGGTGTCAATGGGAGCCGCAAAACTTGCACAGAAAGTGGTGTATGCACGCCTGGTACCGGGATACAAGGGGAATTTAACTTGAACAACTTTCAAGATGGGGTTTTGTCATCAGCTTTTATGGCTGGACTTCTTATTGCTTCTCCTATATTTGCATCATTAGCAAAGAGTGTAAATCCATTTAGGCTCATTGGAGTTGGATTATCAGTTTGGACCTTTGCTACACTTGGTTGTGGTTGTTCATTCGGCTTCTGGTCAATTGCTGTTTTCCGCATGCTAGTCGGTGTTGGCGAGGCTTCGTTTATTAGTCTTGCAGCTCCATTCATCGACGACAATGCCCCAGCTACTCAGAAAACTGCATGGCTTTCTATATTCTACATGTGCATATCAAGTGGATATGCACTTGGCTATGTTTATGGTGGGGTGGTTGGAAGTCACACCAAGTGGCGCTATGCATTTGTTGGGGAGGCAATCCTTATGCTTCCATTTGCTATTCTAGGGTTTGTTATGAAGCCTTTGCAGATGAAAGGTTTTTCTCATCCTGAATCAAAAAAAGCACCGATAGCCGTGGAAACTGCTATTGAAGAAGTTCAAGGTTCAGATATTTCGATGAAGGAAGACGTCAGAGATTCTACCATACAAAAGCCTTCCAATTCAAAAGTGAATCCAATTTCAAGGTTTATGAAAGATATGAAGGTGCTTTTGGGGGATAAGGTTTATGTTGTGAATGTTCTAGGTTACATAGCATACAACTTTGTCATAGGTGCATACTCATATTGGGGGCCTAAGGCTGGTTATAACATATATCATATGAGTAATGCAGATATGATATTTGGAGGTGTCACAATTGCGTGCGGGATATTGGGCACACTAGGAGGAGGCTTTCTTCTGGATTATATGACTGGCACTATCTCTAATGCTTTCAAGCTTCTTTCATCGGTAACGTTAATTGGGGGTGCCTTTTGCTTAGGTGCCTTCTGGATGAAGAATTTGTATGCTTTCCTGGCTCTTTTTGCAATCGGCCAACTACTCGTCTTTGCAACTCAGGGTCCTGTAAACTACGTTTGTCTCCATTGTGTTAAACCTAGCATGAGGCCACTATCTATGGCGATTTCTACAGTTGCAATTCACCTTTTCGGGGATGTACCTTCCGCGCCTCTTGTCGGAGTTCTCCAGGATGCCATTAACAACTGGAGGGCGACCGCTCTTATTCTAACATCTATTTTCTTTCCAGCAGCTCTAATATGGTTTATAGGAATCTTTCTGCACAGCGTGGATAGATTTAACGAAGAAAGTGAGCATCAGATCACCACCAAAACCGATACGTCAAACACAACCCCAATGGATGAAGGGAAGAAAACAGAAACAATTGAATCTGTTGCTGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

525

Amino Acids

56.43

Weight (kDa)

7.02

Isoelectric Point (pI)

34.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 82 - 438 1.3e-30 Major Facilitator Superfamily
OATP PF03137 150 - 389 2.6e-19 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 390 - 454 2.5e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AatII GACGTC 1 cut(s) 807
Acc65I GGTACC 1 cut(s) 239
AccB1I GGYRCC 3 cut(s) 239, 1139, 1154
AccBSI CCGCTC 1 cut(s) 1388
AccII CGCG 1 cut(s) 1337
AciI CCGC 7 cut(s) 53, 81, 202, 454, 1038, 1335, 1386
AclI AACGTT 1 cut(s) 1127
AcoI YGGCCR 1 cut(s) 1204
AcsI RAATTY 2 cut(s) 257, 1171
AcuI CTGAAG 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 804
AdeI CACNNNGTG 2 cut(s) 627, 1456
AfaI GTAC 2 cut(s) 241, 1329
AfiI CCNNNNNNNGG 5 cut(s) 49, 242, 974, 1321, 1346
AflIII ACRYGT 1 cut(s) 561
AgsI TTSAA 9 cut(s) 78, 268, 278, 767, 776, 840, 858, 1108, 1556
AjnI CCWGG 3 cut(s) 235, 1185, 1356
AjuI GAANNNNNNNTTGG 2 cut(s) 869, 901
AluBI AGCT 7 cut(s) 86, 95, 299, 500, 527, 1111, 1421
AluI AGCT 7 cut(s) 86, 95, 299, 500, 527, 1111, 1421
Alw26I GTCTC 2 cut(s) 50, 1256
AoxI GGCC 3 cut(s) 965, 1204, 1277
ApeKI GCWGC 4 cut(s) 83, 92, 497, 1418
ApoI RAATTY 2 cut(s) 257, 1171
Asp700I GAANNNNTTC 1 cut(s) 771
Asp718I GGTACC 1 cut(s) 239
AspLEI GCGC 2 cut(s) 633, 1339
AspS9I GGNCC 4 cut(s) 163, 396, 965, 1232
AsuC2I CCSGG 2 cut(s) 50, 244
AsuHPI GGTGA 2 cut(s) 1304, 1483
AvaII GGWCC 3 cut(s) 163, 396, 1232
AxyI CCTNAGG 1 cut(s) 969
BaeGI GKGCMC 1 cut(s) 1052
BanI GGYRCC 3 cut(s) 239, 1139, 1154
BbsI GAAGAC 1 cut(s) 807
BbvI GCAGC 4 cut(s) 95, 104, 509, 1430
BccI CCATC 1 cut(s) 275
BceAI ACGGC 1 cut(s) 734
BcgI CGANNNNNNTGC 4 cut(s) 445, 479, 500, 534
BciT130I CCWGG 3 cut(s) 237, 1187, 1358
BciVI GTATCC 1 cut(s) 240
BcnI CCSGG 2 cut(s) 50, 244
BcoDI GTCTC 2 cut(s) 50, 1256
BfaI CTAG 5 cut(s) 461, 680, 917, 1055, 1269
BfmI CTRYAG 1 cut(s) 1299
BfoI RGCGCY 1 cut(s) 634
BfuI GTATCC 1 cut(s) 240
BglI GCCNNNNNGGC 1 cut(s) 118
BisI GCNGC 5 cut(s) 84, 93, 202, 498, 1419
BlsI GCNGC 5 cut(s) 85, 94, 203, 499, 1420
Bme1390I CCNGG 5 cut(s) 50, 237, 244, 1187, 1358
Bme18I GGWCC 3 cut(s) 163, 396, 1232
BmgT120I GGNCC 4 cut(s) 163, 396, 965, 1232
BmiI GGNNCC 6 cut(s) 200, 241, 966, 1141, 1156, 1233
BmrFI CCNGG 5 cut(s) 50, 237, 244, 1187, 1358
BmsI GCATC 3 cut(s) 347, 1351, 1492
BpiI GAAGAC 1 cut(s) 807
BpmI CTGGAG 2 cut(s) 1340, 1396
Bpu10I CCTNAGC 1 cut(s) 1150
BpuMI CCSGG 2 cut(s) 50, 244
BsaHI GRCGYC 1 cut(s) 804
BsaJI CCNNGG 3 cut(s) 48, 166, 750
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 242, 974, 1321, 1346
Bse1I ACTGG 2 cut(s) 1090, 1379
Bse21I CCTNAGG 1 cut(s) 969
Bse3DI GCAATG 1 cut(s) 190
BseBI CCWGG 3 cut(s) 237, 1187, 1358
BseDI CCNNGG 3 cut(s) 48, 166, 750
BseGI GGATG 6 cut(s) 59, 721, 1170, 1330, 1366, 1534
BseLI CCNNNNNNNGG 5 cut(s) 49, 242, 974, 1321, 1346
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 545, 1241
BseNI ACTGG 2 cut(s) 1090, 1379
BseRI GAGGAG 1 cut(s) 1074
BseSI GKGCMC 1 cut(s) 1052
BseXI GCAGC 4 cut(s) 95, 104, 509, 1430
BseYI CCCAGC 1 cut(s) 523
BsgI GTGCAG 1 cut(s) 1433
Bsh1236I CGCG 1 cut(s) 1337
Bsh1285I CGRYCG 1 cut(s) 1386
BshFI GGCC 3 cut(s) 967, 1206, 1279
BshNI GGYRCC 3 cut(s) 239, 1139, 1154
BsiEI CGRYCG 1 cut(s) 1386
BsiSI CCGG 3 cut(s) 50, 89, 243
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 5 cut(s) 49, 242, 974, 1321, 1346
BsmAI GTCTC 2 cut(s) 50, 1256
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 3 cut(s) 967, 1206, 1279
Bsp1286I GDGCHC 1 cut(s) 1052
Bsp143I GATC 1 cut(s) 1488
BspACI CCGC 7 cut(s) 53, 81, 202, 454, 1038, 1335, 1386
BspANI GGCC 3 cut(s) 967, 1206, 1279
BspCNI CTCAG 2 cut(s) 544, 1240
BspFNI CGCG 1 cut(s) 1337
BspLI GGNNCC 6 cut(s) 200, 241, 966, 1141, 1156, 1233
BspT107I GGYRCC 3 cut(s) 239, 1139, 1154
BsrBI CCGCTC 1 cut(s) 1388
BsrDI GCAATG 1 cut(s) 190
BsrI ACTGG 2 cut(s) 1090, 1379
BssECI CCNNGG 3 cut(s) 48, 166, 750
BssMI GATC 1 cut(s) 1488
BssNI GRCGYC 1 cut(s) 804
Bst2UI CCWGG 3 cut(s) 237, 1187, 1358
Bst4CI ACNGT 1 cut(s) 1303
BstACI GRCGYC 1 cut(s) 804
BstAPI GCANNNNNTGC 1 cut(s) 210
BstC8I GCNNGC 4 cut(s) 181, 233, 458, 1036
BstDEI CTNAG 4 cut(s) 531, 969, 1150, 1227
BstDSI CCRYGG 1 cut(s) 750
BstF5I GGATG 6 cut(s) 59, 721, 1170, 1330, 1366, 1534
BstFNI CGCG 1 cut(s) 1337
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 2 cut(s) 633, 1339
BstKTI GATC 1 cut(s) 1491
BstMAI GTCTC 2 cut(s) 50, 1256
BstMBI GATC 1 cut(s) 1488
BstMCI CGRYCG 1 cut(s) 1386
BstNI CCWGG 3 cut(s) 237, 1187, 1358
BstNSI RCATGY 2 cut(s) 460, 565
BstSCI CCNGG 5 cut(s) 48, 235, 242, 1185, 1356
BstSFI CTRYAG 1 cut(s) 1299
BstSLI GKGCMC 1 cut(s) 1052
BstUI CGCG 1 cut(s) 1337
BstV1I GCAGC 4 cut(s) 95, 104, 509, 1430
BstV2I GAAGAC 1 cut(s) 807
Bsu36I CCTNAGG 1 cut(s) 969
BsuI GTATCC 1 cut(s) 240
BsuRI GGCC 3 cut(s) 967, 1206, 1279
BtgI CCRYGG 1 cut(s) 750
BtsCI GGATG 6 cut(s) 59, 721, 1170, 1330, 1366, 1534
Cac8I GCNNGC 4 cut(s) 181, 233, 458, 1036
CfoI GCGC 2 cut(s) 633, 1339
Cfr13I GGNCC 4 cut(s) 163, 396, 965, 1232
Csp6I GTAC 2 cut(s) 240, 1328
CviAII CATG 6 cut(s) 64, 102, 457, 543, 562, 1273
CviQI GTAC 2 cut(s) 240, 1328
DdeI CTNAG 4 cut(s) 531, 969, 1150, 1227
DpnI GATC 1 cut(s) 1490
DpnII GATC 1 cut(s) 1488
DraIII CACNNNGTG 2 cut(s) 627, 1456
EaeI YGGCCR 1 cut(s) 1204
EciI GGCGGA 1 cut(s) 68
Eco47I GGWCC 3 cut(s) 163, 396, 1232
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 969
EcoO109I RGGNCCY 2 cut(s) 965, 1232
EcoRII CCWGG 3 cut(s) 235, 1185, 1356
EcoT22I ATGCAT 1 cut(s) 640
FaeI CATG 6 cut(s) 67, 105, 460, 546, 565, 1276
FaqI GGGAC 1 cut(s) 31
FatI CATG 6 cut(s) 63, 101, 456, 542, 561, 1272
FauI CCCGC 1 cut(s) 1031
FauNDI CATATG 1 cut(s) 993
Fnu4HI GCNGC 5 cut(s) 84, 93, 202, 498, 1419
FokI GGATG 6 cut(s) 46, 708, 1177, 1337, 1373, 1541
Fsp4HI GCNGC 5 cut(s) 84, 93, 202, 498, 1419
FspBI CTAG 5 cut(s) 461, 680, 917, 1055, 1269
GlaI GCGC 2 cut(s) 632, 1338
GluI GCNGC 5 cut(s) 84, 93, 202, 498, 1419
GsaI CCCAGC 1 cut(s) 527
GsuI CTGGAG 2 cut(s) 1340, 1396
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 3 cut(s) 967, 1206, 1279
HapII CCGG 3 cut(s) 50, 89, 243
HhaI GCGC 2 cut(s) 633, 1339
Hin1I GRCGYC 1 cut(s) 804
Hin1II CATG 6 cut(s) 67, 105, 460, 546, 565, 1276
Hin6I GCGC 2 cut(s) 631, 1337
HinP1I GCGC 2 cut(s) 631, 1337
HindIII AAGCTT 1 cut(s) 1109
HinfI GANTC 5 cut(s) 728, 812, 847, 1440, 1556
HpaII CCGG 3 cut(s) 50, 89, 243
HphI GGTGA 2 cut(s) 1304, 1483
Hpy166II GTNNAC 3 cut(s) 108, 163, 1240
Hpy188I TCNGA 6 cut(s) 28, 534, 784, 809, 1349, 1488
Hpy188III TCNNGA 4 cut(s) 278, 725, 1073, 1162
Hpy8I GTNNAC 3 cut(s) 108, 163, 1240
Hpy99I CGWCG 1 cut(s) 515
HpyAV CCTTC 7 cut(s) 108, 790, 840, 871, 1168, 1341, 1526
HpyCH4III ACNGT 1 cut(s) 1303
HpyCH4IV ACGT 4 cut(s) 804, 1127, 1245, 1508
HpyF3I CTNAG 4 cut(s) 531, 969, 1150, 1227
HpySE526I ACGT 4 cut(s) 804, 1127, 1245, 1508
Hsp92I GRCGYC 1 cut(s) 804
Hsp92II CATG 6 cut(s) 67, 105, 460, 546, 565, 1276
HspAI GCGC 2 cut(s) 631, 1337
KpnI GGTACC 1 cut(s) 243
Kzo9I GATC 1 cut(s) 1488
LmnI GCTCC 4 cut(s) 91, 100, 198, 505
Lsp1109I GCAGC 4 cut(s) 95, 104, 509, 1430
LweI GCATC 3 cut(s) 347, 1351, 1492
MaeI CTAG 5 cut(s) 461, 680, 917, 1055, 1269
MaeII ACGT 4 cut(s) 804, 1127, 1245, 1508
MaeIII GTNAC 4 cut(s) 617, 920, 1024, 1123
MalI GATC 1 cut(s) 1490
MbiI CCGCTC 1 cut(s) 1388
MboI GATC 1 cut(s) 1488
MboII GAAGA 7 cut(s) 41, 779, 812, 1061, 1180, 1484, 1549
MfeI CAATTG 3 cut(s) 441, 1029, 1551
MhlI GDGCHC 1 cut(s) 1052
MluCI AATT 9 cut(s) 257, 441, 835, 852, 1029, 1131, 1171, 1308, 1551
MmeI TCCRAC 3 cut(s) 361, 592, 1327
Mph1103I ATGCAT 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 7 cut(s) 144, 152, 261, 1130, 1263, 1368, 1467
MspI CCGG 3 cut(s) 50, 89, 243
MspR9I CCNGG 5 cut(s) 50, 237, 244, 1187, 1358
MunI CAATTG 3 cut(s) 441, 1029, 1551
MvaI CCWGG 3 cut(s) 237, 1187, 1358
MvnI CGCG 1 cut(s) 1337
NciI CCSGG 2 cut(s) 50, 244
NdeI CATATG 1 cut(s) 993
NdeII GATC 1 cut(s) 1488
NlaIII CATG 6 cut(s) 67, 105, 460, 546, 565, 1276
NlaIV GGNNCC 6 cut(s) 200, 241, 966, 1141, 1156, 1233
NmuCI GTSAC 2 cut(s) 617, 1024
NsiI ATGCAT 1 cut(s) 640
NspI RCATGY 2 cut(s) 460, 565
PaeI GCATGC 1 cut(s) 460
PciI ACATGT 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PdmI GAANNNNTTC 1 cut(s) 771
PfeI GAWTC 5 cut(s) 728, 812, 847, 1440, 1556
PfoI TCCNGGA 1 cut(s) 1356
PkrI GCNGC 5 cut(s) 85, 94, 203, 499, 1420
PpuMI RGGWCCY 1 cut(s) 1232
PscI ACATGT 1 cut(s) 561
PsiI TTATAA 1 cut(s) 981
Psp1406I AACGTT 1 cut(s) 1127
Psp5II RGGWCCY 1 cut(s) 1232
Psp6I CCWGG 3 cut(s) 235, 1185, 1356
PspFI CCCAGC 1 cut(s) 523
PspGI CCWGG 3 cut(s) 235, 1185, 1356
PspN4I GGNNCC 6 cut(s) 200, 241, 966, 1141, 1156, 1233
PspPI GGNCC 4 cut(s) 163, 396, 965, 1232
PspPPI RGGWCCY 1 cut(s) 1232
RsaI GTAC 2 cut(s) 241, 1329
RsaNI GTAC 2 cut(s) 240, 1328
SaqAI TTAA 7 cut(s) 144, 152, 261, 1130, 1263, 1368, 1467
SatI GCNGC 5 cut(s) 84, 93, 202, 498, 1419
Sau3AI GATC 1 cut(s) 1488
Sau96I GGNCC 4 cut(s) 163, 396, 965, 1232
ScrFI CCNGG 5 cut(s) 50, 237, 244, 1187, 1358
SduI GDGCHC 1 cut(s) 1052
SfaNI GCATC 3 cut(s) 347, 1351, 1492
SfcI CTRYAG 1 cut(s) 1299
SinI GGWCC 3 cut(s) 163, 396, 1232
SphI GCATGC 1 cut(s) 460
Sse9I AATT 9 cut(s) 257, 441, 835, 852, 1029, 1131, 1171, 1308, 1551
SsiI CCGC 7 cut(s) 53, 81, 202, 454, 1038, 1335, 1386
SspMI CTAG 5 cut(s) 461, 680, 917, 1055, 1269
StyD4I CCNGG 5 cut(s) 48, 235, 242, 1185, 1356
TaaI ACNGT 1 cut(s) 1303
TaiI ACGT 4 cut(s) 807, 1130, 1248, 1511
TaqI TCGA 2 cut(s) 510, 791
TaqII GACCGA 1 cut(s) 180
TasI AATT 9 cut(s) 257, 441, 835, 852, 1029, 1131, 1171, 1308, 1551
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 5 cut(s) 728, 812, 847, 1440, 1556
Tru1I TTAA 7 cut(s) 144, 152, 261, 1130, 1263, 1368, 1467
Tru9I TTAA 7 cut(s) 144, 152, 261, 1130, 1263, 1368, 1467
TseFI GTSAC 2 cut(s) 617, 1024
TseI GCWGC 4 cut(s) 83, 92, 497, 1418
Tsp45I GTSAC 2 cut(s) 617, 1024
VpaK11BI GGWCC 3 cut(s) 163, 396, 1232
XapI RAATTY 2 cut(s) 257, 1171
XceI RCATGY 2 cut(s) 460, 565
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 5 cut(s) 461, 680, 917, 1055, 1269
ZraI GACGTC 1 cut(s) 805
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.