Rorug06G0044900

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001054
Physical Location & Seq
Forward (+)
5913231 .. 5918331
5101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0044900.1

Sequence Viewer

Length: 1407 bp
ATGGTACAGATGTGGCTGCATTTGCTTCTTTTATTGCTTCTTTCTGGTGCTGCAACGTCACAAAACTTCACCAACTCCTTGCCTGGTTACCCCGGTGAACTCCCCTTTACTCTCGAAACTGGATACGTGGGTGTGGGAGATTTGGAAGAGGTGCAGCTTTTCTACTACTTCATAGAGTCTGAGAGGAACCCTGTAAAGGACCCTCTTCTGCTCTGGCTCAATGGAGGCCCCGGTTGTTCCGCTTTCTCTGGCCTAGTATATGAAATTGGTCCTCTAACTTTCGACTATGCAGCCTTCAATGGCAGCCTGCCTACTTTACTGGACAACCCATTCTCATGGACTCAGATTGCCAGCGTAATTTTTGTAGATGCACCTGTTGGCACCGGTTTCTCCTATTCAACTACTCAGGAAGGTTTTTACACCGACGACACAAAATCAGCAGAAGACGTATACCAATTTCTCCGGAAGTGGTTGATCAATCACCCTAAGTTTAGCAAAAATCCACTCTACATCACTGGAGATTCCTATGCAGGCATCATTGCTCCTCAAGTCACCCTTAAAGTATCAAATGGTAATCTCGATAGAGTCAAGCCACCGATGAATCTCATGGGATATGTACTTGGAAATCCGGTAACAAATATACATAAAGATGAAAATTCAAGAATCAAGTTTTACCACCGCCTCACACTTATATCAAGTGAACTTTATGAGTCCATTACAACAAGTTGCAACGGGGAATATGTTTATCCGGATCCAGATAATGCAGATTGCGTGGAAGACATTGGACTTGTGGCAGAGTGCACCATAAAAGTAAACGATGTGCAAGTTTTGGAACCAAAATGTAGCTCGGCATCCCCAAGACCATCTAAAGGAATAAAAAGGGAACCAAAATTTTTTGAAGACATCCCTGATGAAATCATGATTCAATCACCAATGGAACGCCGAGAAAATTGGTGCCGGACAAACAATTATGTGTTATCTTATATGTGGGCAAACGATGAAAGGGTCCAAGAAGCACTTAGCATTCGAAATGGTACTATAACAGATTGGAAGAGGTGCAATAAGAGCTTGGCTTATGACTATAATCTAGTGACCACGGTTCCTTACCATGGAGAACTCATTCGAAGAGGCTACCGAAGTTTGATATACAGTGGTGATCATGATGGGTTGATTCCATATACGGGTACAATAGCATGGATAGCTACTCTTAACTTGACTACCGTCGATGGATGGAGACCATGGTATGTCGACGGACAAGTTGCCGGATTCTCCGAGAAGTATGCAAATCTAGTATCAGAAGGATTGACGTTTGCAACTGTGAAGGGAGGGGGTCACACAGCTTCAGAGTACATGCCTAAGCAATGTCTTGAAATGTTGGATAGATGGCTCTCTTACTATTCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

468

Amino Acids

52.74

Weight (kDa)

4.9

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 28 - 464 2.8e-121 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 380, 954
AccI GTMKAC 2 cut(s) 450, 1248
AccIII TCCGGA 2 cut(s) 462, 748
AciI CCGC 2 cut(s) 240, 679
AclWI GGATC 2 cut(s) 746, 759
AcsI RAATTY 2 cut(s) 655, 890
AcuI CTGAAG 1 cut(s) 1326
AfaI GTAC 5 cut(s) 6, 618, 1036, 1186, 1349
AfiI CCNNNNNNNGG 4 cut(s) 196, 869, 1109, 1181
AgeI ACCGGT 1 cut(s) 383
AgsI TTSAA 6 cut(s) 298, 399, 660, 899, 926, 1370
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 5 cut(s) 157, 846, 1068, 1202, 1340
AluI AGCT 5 cut(s) 157, 846, 1068, 1202, 1340
Alw21I GWGCWC 1 cut(s) 803
Alw26I GTCTC 1 cut(s) 1228
Alw44I GTGCAC 1 cut(s) 799
AlwI GGATC 2 cut(s) 746, 759
Aor13HI TCCGGA 2 cut(s) 462, 748
AoxI GGCC 2 cut(s) 226, 250
ApaLI GTGCAC 1 cut(s) 799
ApeKI GCWGC 5 cut(s) 16, 50, 154, 290, 303
ApoI RAATTY 2 cut(s) 655, 890
AsiGI ACCGGT 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 1119
AspS9I GGNCC 4 cut(s) 199, 227, 269, 1006
AsuC2I CCSGG 2 cut(s) 93, 231
AsuHPI GGTGA 6 cut(s) 61, 107, 473, 544, 921, 1166
AsuII TTCGAA 2 cut(s) 1027, 1123
AvaII GGWCC 3 cut(s) 199, 269, 1006
BaeGI GKGCMC 1 cut(s) 803
BaeI ACNNNNGTAYC 3 cut(s) 29, 115, 148
BamHI GGATCC 1 cut(s) 751
BanI GGYRCC 2 cut(s) 380, 954
BbsI GAAGAC 3 cut(s) 450, 783, 906
Bbv12I GWGCWC 1 cut(s) 803
BbvI GCAGC 5 cut(s) 3, 37, 166, 302, 315
BccI CCATC 5 cut(s) 871, 1157, 1220, 1224, 1377
BcgI CGANNNNNNTGC 2 cut(s) 1262, 1296
BciT130I CCWGG 1 cut(s) 84
BciVI GTATCC 1 cut(s) 116
BclI TGATCA 2 cut(s) 474, 1156
BcnI CCSGG 2 cut(s) 93, 231
BcoDI GTCTC 1 cut(s) 1228
BfaI CTAG 3 cut(s) 254, 1088, 1289
BfuI GTATCC 1 cut(s) 116
BisI GCNGC 5 cut(s) 17, 51, 155, 291, 304
BlsI GCNGC 5 cut(s) 18, 52, 156, 292, 305
Bme1390I CCNGG 3 cut(s) 84, 93, 231
Bme18I GGWCC 3 cut(s) 199, 269, 1006
BmgT120I GGNCC 4 cut(s) 199, 227, 269, 1006
BmrFI CCNGG 3 cut(s) 84, 93, 231
BmsI GCATC 3 cut(s) 358, 543, 860
BoxI GACNNNNGTC 1 cut(s) 1220
BpiI GAAGAC 3 cut(s) 450, 783, 906
BpmI CTGGAG 1 cut(s) 537
Bpu10I CCTNAGC 1 cut(s) 1356
Bpu14I TTCGAA 2 cut(s) 1027, 1123
BpuEI CTTGAG 1 cut(s) 531
BpuMI CCSGG 2 cut(s) 93, 231
BsaAI YACGTR 1 cut(s) 127
BsaI GGTCTC 1 cut(s) 1228
BsaJI CCNNGG 5 cut(s) 91, 229, 1095, 1108, 1238
BsaWI WCCGGW 4 cut(s) 383, 462, 628, 748
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 869, 1109, 1181
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 3 cut(s) 124, 324, 520
Bse3DI GCAATG 2 cut(s) 537, 1367
BseAI TCCGGA 2 cut(s) 462, 748
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 5 cut(s) 91, 229, 1095, 1108, 1238
BseGI GGATG 3 cut(s) 851, 903, 1235
BseLI CCNNNNNNNGG 4 cut(s) 196, 869, 1109, 1181
BseMI GCAATG 2 cut(s) 537, 1367
BseMII CTCAG 3 cut(s) 171, 356, 419
BseNI ACTGG 3 cut(s) 124, 324, 520
BseRI GAGGAG 1 cut(s) 534
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 5 cut(s) 3, 37, 166, 302, 315
BsgI GTGCAG 1 cut(s) 173
BshFI GGCC 2 cut(s) 228, 252
BshNI GGYRCC 2 cut(s) 380, 954
BshTI ACCGGT 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 803
BsiSI CCGG 8 cut(s) 93, 231, 384, 463, 629, 749, 958, 1263
BslI CCNNNNNNNGG 4 cut(s) 196, 869, 1109, 1181
BsmAI GTCTC 1 cut(s) 1228
BsmI GAATGC 1 cut(s) 1023
BsnI GGCC 2 cut(s) 228, 252
Bso31I GGTCTC 1 cut(s) 1228
Bsp119I TTCGAA 2 cut(s) 1027, 1123
Bsp1286I GDGCHC 1 cut(s) 803
Bsp13I TCCGGA 2 cut(s) 462, 748
Bsp143I GATC 3 cut(s) 474, 751, 1156
Bsp19I CCATGG 2 cut(s) 1108, 1238
BspACI CCGC 2 cut(s) 240, 679
BspANI GGCC 2 cut(s) 228, 252
BspCNI CTCAG 3 cut(s) 172, 355, 418
BspEI TCCGGA 2 cut(s) 462, 748
BspHI TCATGA 2 cut(s) 918, 1159
BspPI GGATC 2 cut(s) 746, 759
BspT104I TTCGAA 2 cut(s) 1027, 1123
BspT107I GGYRCC 2 cut(s) 380, 954
BspTNI GGTCTC 1 cut(s) 1228
BsrDI GCAATG 2 cut(s) 537, 1367
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 3 cut(s) 124, 324, 520
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 5 cut(s) 91, 229, 1095, 1108, 1238
BssMI GATC 3 cut(s) 474, 751, 1156
BssNAI GTATAC 1 cut(s) 451
BssT1I CCWWGG 2 cut(s) 1108, 1238
Bst1107I GTATAC 1 cut(s) 451
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 4 cut(s) 1099, 1151, 1222, 1318
Bst6I CTCTTC 4 cut(s) 141, 210, 1046, 1120
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 2 cut(s) 1027, 1123
BstC8I GCNNGC 3 cut(s) 308, 352, 532
BstDEI CTNAG 6 cut(s) 180, 342, 405, 486, 1019, 1356
BstDSI CCRYGG 3 cut(s) 1095, 1108, 1238
BstEII GGTNACC 1 cut(s) 86
BstF5I GGATG 3 cut(s) 851, 903, 1235
BstKTI GATC 3 cut(s) 477, 754, 1159
BstMAI GTCTC 1 cut(s) 1228
BstMBI GATC 3 cut(s) 474, 751, 1156
BstMWI GCNNNNNNNGC 3 cut(s) 22, 1065, 1199
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 1 cut(s) 1354
BstPAI GACNNNNGTC 1 cut(s) 1220
BstPI GGTNACC 1 cut(s) 86
BstSCI CCNGG 3 cut(s) 82, 91, 229
BstSLI GKGCMC 1 cut(s) 803
BstV1I GCAGC 5 cut(s) 3, 37, 166, 302, 315
BstV2I GAAGAC 3 cut(s) 450, 783, 906
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 336
BstYI RGATCY 1 cut(s) 751
BstZ17I GTATAC 1 cut(s) 451
BsuI GTATCC 1 cut(s) 116
BsuRI GGCC 2 cut(s) 228, 252
BtgI CCRYGG 3 cut(s) 1095, 1108, 1238
BtsCI GGATG 3 cut(s) 851, 903, 1235
BtsIMutI CAGTG 2 cut(s) 513, 1156
Cac8I GCNNGC 3 cut(s) 308, 352, 532
CciI TCATGA 2 cut(s) 918, 1159
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 4 cut(s) 199, 227, 269, 1006
Csp6I GTAC 5 cut(s) 5, 617, 1035, 1185, 1348
CspAI ACCGGT 1 cut(s) 383
CviAII CATG 8 cut(s) 336, 607, 919, 1109, 1160, 1194, 1239, 1351
CviQI GTAC 5 cut(s) 5, 617, 1035, 1185, 1348
DdeI CTNAG 6 cut(s) 180, 342, 405, 486, 1019, 1356
DpnI GATC 3 cut(s) 476, 753, 1158
DpnII GATC 3 cut(s) 474, 751, 1156
Eam1104I CTCTTC 4 cut(s) 141, 210, 1046, 1120
EarI CTCTTC 4 cut(s) 141, 210, 1046, 1120
Eco130I CCWWGG 2 cut(s) 1108, 1238
Eco31I GGTCTC 1 cut(s) 1228
Eco47I GGWCC 3 cut(s) 199, 269, 1006
Eco57I CTGAAG 1 cut(s) 1326
Eco91I GGTNACC 1 cut(s) 86
EcoO109I RGGNCCY 2 cut(s) 199, 227
EcoO65I GGTNACC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 82
EcoT14I CCWWGG 2 cut(s) 1108, 1238
ErhI CCWWGG 2 cut(s) 1108, 1238
FaeI CATG 8 cut(s) 339, 610, 922, 1112, 1163, 1197, 1242, 1354
FalI AAGNNNNNCTT 4 cut(s) 540, 572, 1002, 1034
FatI CATG 8 cut(s) 335, 606, 918, 1108, 1159, 1193, 1238, 1350
FbaI TGATCA 2 cut(s) 474, 1156
FblI GTMKAC 2 cut(s) 450, 1248
Fnu4HI GCNGC 5 cut(s) 17, 51, 155, 291, 304
FokI GGATG 3 cut(s) 838, 890, 1242
Fsp4HI GCNGC 5 cut(s) 17, 51, 155, 291, 304
FspBI CTAG 3 cut(s) 254, 1088, 1289
GluI GCNGC 5 cut(s) 17, 51, 155, 291, 304
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 2 cut(s) 228, 252
HapII CCGG 8 cut(s) 93, 231, 384, 463, 629, 749, 958, 1263
Hin1II CATG 8 cut(s) 339, 610, 922, 1112, 1163, 1197, 1242, 1354
HincII GTYRAC 1 cut(s) 1249
HindII GTYRAC 1 cut(s) 1249
HpaII CCGG 8 cut(s) 93, 231, 384, 463, 629, 749, 958, 1263
HphI GGTGA 6 cut(s) 61, 107, 473, 544, 921, 1166
Hpy166II GTNNAC 6 cut(s) 98, 451, 701, 801, 814, 1249
Hpy188I TCNGA 5 cut(s) 181, 345, 1273, 1297, 1345
Hpy8I GTNNAC 6 cut(s) 98, 451, 701, 801, 814, 1249
Hpy99I CGWCG 3 cut(s) 428, 1226, 1253
HpyAV CCTTC 4 cut(s) 304, 404, 1292, 1315
HpyCH4III ACNGT 4 cut(s) 1099, 1151, 1222, 1318
HpyCH4IV ACGT 4 cut(s) 56, 126, 447, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 1065, 1199
HpyF3I CTNAG 6 cut(s) 180, 342, 405, 486, 1019, 1356
HpySE526I ACGT 4 cut(s) 56, 126, 447, 1307
Hsp92II CATG 8 cut(s) 339, 610, 922, 1112, 1163, 1197, 1242, 1354
Kpn2I TCCGGA 2 cut(s) 462, 748
Ksp22I TGATCA 2 cut(s) 474, 1156
Kzo9I GATC 3 cut(s) 474, 751, 1156
LmnI GCTCC 1 cut(s) 547
Lsp1109I GCAGC 5 cut(s) 3, 37, 166, 302, 315
LweI GCATC 3 cut(s) 358, 543, 860
MaeI CTAG 3 cut(s) 254, 1088, 1289
MaeII ACGT 4 cut(s) 56, 126, 447, 1307
MaeIII GTNAC 6 cut(s) 57, 86, 550, 631, 1090, 1331
MalI GATC 3 cut(s) 476, 753, 1158
MboI GATC 3 cut(s) 474, 751, 1156
MboII GAAGA 7 cut(s) 158, 197, 455, 788, 911, 1063, 1137
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 1 cut(s) 803
MluCI AATT 7 cut(s) 264, 357, 455, 655, 890, 949, 967
MlyI GAGTC 4 cut(s) 185, 334, 594, 719
MmeI TCCRAC 1 cut(s) 1356
MroI TCCGGA 2 cut(s) 462, 748
MroXI GAANNNNTTC 1 cut(s) 1119
MseI TTAA 2 cut(s) 558, 1209
MslI CAYNNNNRTG 2 cut(s) 334, 648
MspI CCGG 8 cut(s) 93, 231, 384, 463, 629, 749, 958, 1263
MspR9I CCNGG 3 cut(s) 84, 93, 231
Mva1269I GAATGC 1 cut(s) 1023
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 22, 1065, 1199
NciI CCSGG 2 cut(s) 93, 231
NcoI CCATGG 2 cut(s) 1108, 1238
NdeII GATC 3 cut(s) 474, 751, 1156
NlaIII CATG 8 cut(s) 339, 610, 922, 1112, 1163, 1197, 1242, 1354
NmeAIII GCCGAG 2 cut(s) 827, 968
NmuCI GTSAC 4 cut(s) 57, 550, 1090, 1331
NspI RCATGY 1 cut(s) 1354
NspV TTCGAA 2 cut(s) 1027, 1123
PagI TCATGA 2 cut(s) 918, 1159
PctI GAATGC 1 cut(s) 1023
PdmI GAANNNNTTC 1 cut(s) 1119
PfeI GAWTC 6 cut(s) 521, 601, 663, 922, 1171, 1266
PinAI ACCGGT 1 cut(s) 383
PkrI GCNGC 5 cut(s) 18, 52, 156, 292, 305
PleI GAGTC 4 cut(s) 184, 334, 593, 718
PpsI GAGTC 4 cut(s) 184, 334, 593, 718
Ppu21I YACGTR 1 cut(s) 127
PpuMI RGGWCCY 1 cut(s) 199
PshAI GACNNNNGTC 1 cut(s) 1220
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 82
PspEI GGTNACC 1 cut(s) 86
PspGI CCWGG 1 cut(s) 82
PspPI GGNCC 4 cut(s) 199, 227, 269, 1006
PspPPI RGGWCCY 1 cut(s) 199
PsuI RGATCY 1 cut(s) 751
RsaI GTAC 5 cut(s) 6, 618, 1036, 1186, 1349
RsaNI GTAC 5 cut(s) 5, 617, 1035, 1185, 1348
RseI CAYNNNNRTG 2 cut(s) 334, 648
SalI GTCGAC 1 cut(s) 1247
SaqAI TTAA 2 cut(s) 558, 1209
SatI GCNGC 5 cut(s) 17, 51, 155, 291, 304
Sau3AI GATC 3 cut(s) 474, 751, 1156
Sau96I GGNCC 4 cut(s) 199, 227, 269, 1006
SchI GAGTC 4 cut(s) 185, 334, 594, 719
ScrFI CCNGG 3 cut(s) 84, 93, 231
SduI GDGCHC 1 cut(s) 803
SfaNI GCATC 3 cut(s) 358, 543, 860
SfuI TTCGAA 2 cut(s) 1027, 1123
SinI GGWCC 3 cut(s) 199, 269, 1006
SmiMI CAYNNNNRTG 2 cut(s) 334, 648
SmlI CTYRAG 1 cut(s) 546
SmoI CTYRAG 1 cut(s) 546
Sse9I AATT 7 cut(s) 264, 357, 455, 655, 890, 949, 967
SsiI CCGC 2 cut(s) 240, 679
SspMI CTAG 3 cut(s) 254, 1088, 1289
StyD4I CCNGG 3 cut(s) 82, 91, 229
StyI CCWWGG 2 cut(s) 1108, 1238
TaaI ACNGT 4 cut(s) 1099, 1151, 1222, 1318
TaiI ACGT 4 cut(s) 59, 129, 450, 1310
TaqI TCGA 7 cut(s) 114, 282, 579, 1027, 1123, 1224, 1248
TasI AATT 7 cut(s) 264, 357, 455, 655, 890, 949, 967
TatI WGTACW 2 cut(s) 616, 1347
TfiI GAWTC 6 cut(s) 521, 601, 663, 922, 1171, 1266
Tru1I TTAA 2 cut(s) 558, 1209
Tru9I TTAA 2 cut(s) 558, 1209
TscAI CASTG 2 cut(s) 520, 1156
TseFI GTSAC 4 cut(s) 57, 550, 1090, 1331
TseI GCWGC 5 cut(s) 16, 50, 154, 290, 303
Tsp45I GTSAC 4 cut(s) 57, 550, 1090, 1331
TspDTI ATGAA 6 cut(s) 160, 276, 614, 666, 927, 1014
TspGWI ACGGA 1 cut(s) 1266
TspRI CASTG 2 cut(s) 520, 1156
VneI GTGCAC 1 cut(s) 799
VpaK11BI GGWCC 3 cut(s) 199, 269, 1006
XapI RAATTY 2 cut(s) 655, 890
XceI RCATGY 1 cut(s) 1354
XmiI GTMKAC 2 cut(s) 450, 1248
XmnI GAANNNNTTC 1 cut(s) 1119
XspI CTAG 3 cut(s) 254, 1088, 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.