FvH4_2g10950

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001054
Physical Location & Seq
Reverse (-)
9591926 .. 9602350
10425 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10950.t1

Sequence Viewer

Length: 1536 bp
ATGATCGTTTCTTCAATCGAACAACCTTCGTGGTTTTCCGCCAAGAAGTTACTTGTTATATTTTGTGTGATTAACTTGATAAATTATGTGGACCGAGGAGCAATTGCAAGCAATGGTGTGAATGGGACTCTTGGAACTTGTACTGAGGGTGGTACATGTACATCTGGCACCGGCATACAGGGGGATTTTGACTTGAGCAATTTTGAAGATGGATGCCTGTCATCTGCTTTTATGGTTGGCCTTCTGGTGGCGTCTCCGATTTTTGCTTCATTAGCAAAGAGTGTAAATCCATTTAGACTCATTGGAGTTGGATTGTCAGTTTGGACATTTGCTGTAGTTGGTTGTGGTATTTCATTCAACTTCTGGTCCATTACCATCTGCCGCATGCTAGTTGGTGTTGGCGAGGCTTCATTTATTAGTCTTGCAGCTCCATTCATTGATGATAATGCCCCAGCTGCTCAGAAAACTGTGTGGCTTTCAATATTCTACATGTGCATACCATCTGGATATGCACTTGGTTATGTATATGGTGGGTTGGTGGGAAGTTATTCCAATTGGCGTTTTGCATTCTGGGGCGAGGCAATCCTGATGCTTCCGTTTGCTATTCTAGGATTTGTTATGAAGCCTTTGCAGCTTAAAGGTTTCATGCCTGCTGAATCGAAAAAAGTGACAGGAATGGAGACGGGTGTCTCAGAAGTTCAAGATTCAGGTGTTTTGAATGGTAAAGACGGCTCATTTTCAGTGGAGGGAGATGTCAAATATTCTACCTTAGATGACCAGAATGGGGTGAACTTTGCAGCGAAGATAAAGGTTCAAATTTCAAGGTTTACAAAAGATATAAAGGTGCTTTTGGCTGAGAAGGTTTATGTTGTTAATGTTCTAGGTTACATCGCGTACAACTTTGTAATCGGTGCATACTCATATTGGGGGCCTAAGGCTGGTTATAATATATACAATATGACTGACGCAGATTTGATATTTGGAGGTATTACAATTGTATGCGGAATATTGGGTACAGTAGCAGGAGGTTTTCTTTTGGATTTCATGATCAGCAGTATCCCTAATGCTTTTAAGCTTCTTTCAGCTGCTACGTTTTTTGGGGCAATATTTTGCTTCAGTGCATTTTGCTTCTCAAACATGTATGCTTTCCTAGCCCTGTTTTCAGTTGGTGAATTACTAGTCTTTGCAACTCAGGGTCCTGTAAATTACATTTGTCTGCACTGTGTCAAACCTAGTATGAGACCACTAGCTATGGCCATGTCTACTGTTTCTATTCATCTTTTTGGGGATGTACCTTCCTCCCCTCTTGTTGGAGTTCTCCAGGATTACATCAACAATTGGAGGGCGACTGCTCTTATTCTAACATCTGTATTCATTCCAGCGGCTGGTATATGGTTTATAGGAGTTTTTCTGCCCAGTGTGGATAGATTTGATGAAGAAAGTGAGGATCTTATCTCAACAACTGAAAGGTCAAGCACAACTCCTCTTCTTGAAGGGACGAAGACACAATCGACTGGATCTATTGCTGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

512

Amino Acids

54.87

Weight (kDa)

4.89

Isoelectric Point (pI)

26.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 58 - 424 4.1e-29 Major Facilitator Superfamily
OATP PF03137 123 - 376 7.1e-17 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 375 - 442 5.1e-07 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 945
AccB1I GGYRCC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 1385
AccI GTMKAC 1 cut(s) 1262
AccII CGCG 1 cut(s) 893
AciI CCGC 4 cut(s) 39, 382, 1002, 1382
AclWI GGATC 2 cut(s) 1455, 1525
AcoI YGGCCR 1 cut(s) 1254
AcsI RAATTY 1 cut(s) 816
AcuI CTGAAG 1 cut(s) 1099
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 6 cut(s) 142, 154, 160, 896, 1015, 1293
AfiI CCNNNNNNNGG 5 cut(s) 247, 784, 938, 1310, 1385
AflIII ACRYGT 3 cut(s) 155, 489, 1137
AgsI TTSAA 9 cut(s) 15, 206, 358, 480, 701, 718, 815, 822, 1493
AhlI ACTAGT 1 cut(s) 1177
AjnI CCWGG 1 cut(s) 1320
AluBI AGCT 6 cut(s) 428, 455, 634, 1075, 1085, 1250
AluI AGCT 6 cut(s) 428, 455, 634, 1075, 1085, 1250
Alw26I GTCTC 4 cut(s) 258, 674, 694, 1234
AlwI GGATC 2 cut(s) 1455, 1525
AlwNI CAGNNNCTG 1 cut(s) 1385
AoxI GGCC 3 cut(s) 238, 929, 1254
ApeKI GCWGC 5 cut(s) 425, 455, 631, 797, 1085
ApoI RAATTY 1 cut(s) 816
Asp700I GAANNNNTTC 1 cut(s) 547
AspS9I GGNCC 4 cut(s) 91, 366, 929, 1196
AsuHPI GGTGA 2 cut(s) 799, 1181
AvaII GGWCC 3 cut(s) 91, 366, 1196
AxyI CCTNAGG 1 cut(s) 933
BalI TGGCCA 1 cut(s) 1256
BanI GGYRCC 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 1508
BbvI GCAGC 5 cut(s) 437, 442, 643, 809, 1072
BccI CCATC 3 cut(s) 203, 383, 508
BceAI ACGGC 1 cut(s) 745
BciT130I CCWGG 1 cut(s) 1322
BciVI GTATCC 1 cut(s) 1067
BclI TGATCA 1 cut(s) 1047
BcoDI GTCTC 4 cut(s) 258, 674, 694, 1234
BcuI ACTAGT 1 cut(s) 1177
BfaI CTAG 7 cut(s) 389, 608, 881, 1151, 1178, 1233, 1247
BfmI CTRYAG 1 cut(s) 333
BfuI GTATCC 1 cut(s) 1067
BisI GCNGC 7 cut(s) 382, 426, 456, 632, 798, 1086, 1383
BlsI GCNGC 7 cut(s) 383, 427, 457, 633, 799, 1087, 1384
Bme1390I CCNGG 1 cut(s) 1322
Bme18I GGWCC 3 cut(s) 91, 366, 1196
BmgT120I GGNCC 4 cut(s) 91, 366, 929, 1196
BmiI GGNNCC 3 cut(s) 169, 930, 1197
BmrFI CCNGG 1 cut(s) 1322
BmrI ACTGGG 1 cut(s) 1410
BmsI GCATC 2 cut(s) 203, 579
BmuI ACTGGG 1 cut(s) 1410
BoxI GACNNNNGTC 1 cut(s) 686
BpiI GAAGAC 1 cut(s) 1508
BpmI CTGGAG 1 cut(s) 1304
BpuEI CTTGAG 1 cut(s) 214
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 1234
BsaJI CCNNGG 1 cut(s) 94
Bsc4I CCNNNNNNNGG 5 cut(s) 247, 784, 938, 1310, 1385
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 1416, 1519
Bse21I CCTNAGG 1 cut(s) 933
Bse3DI GCAATG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 1322
BseDI CCNNGG 1 cut(s) 94
BseGI GGATG 2 cut(s) 218, 1294
BseLI CCNNNNNNNGG 5 cut(s) 247, 784, 938, 1310, 1385
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 5 cut(s) 135, 473, 705, 846, 1205
BseNI ACTGG 2 cut(s) 1416, 1519
BseRI GAGGAG 2 cut(s) 111, 1473
BseXI GCAGC 5 cut(s) 437, 442, 643, 809, 1072
BseYI CCCAGC 1 cut(s) 451
BsgI GTGCAG 1 cut(s) 1202
Bsh1236I CGCG 1 cut(s) 893
BshFI GGCC 3 cut(s) 240, 931, 1256
BshNI GGYRCC 1 cut(s) 167
BsiSI CCGG 1 cut(s) 171
BslFI GGGAC 2 cut(s) 139, 1510
BslI CCNNNNNNNGG 5 cut(s) 247, 784, 938, 1310, 1385
BsmAI GTCTC 4 cut(s) 258, 674, 694, 1234
BsmBI CGTCTC 2 cut(s) 258, 674
BsmFI GGGAC 2 cut(s) 139, 1510
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 3 cut(s) 240, 931, 1256
Bso31I GGTCTC 1 cut(s) 1234
Bsp1407I TGTACA 1 cut(s) 158
Bsp143I GATC 4 cut(s) 3, 1047, 1447, 1517
BspACI CCGC 4 cut(s) 39, 382, 1002, 1382
BspANI GGCC 3 cut(s) 240, 931, 1256
BspCNI CTCAG 5 cut(s) 136, 472, 704, 847, 1204
BspFNI CGCG 1 cut(s) 893
BspHI TCATGA 1 cut(s) 1044
BspLI GGNNCC 3 cut(s) 169, 930, 1197
BspPI GGATC 2 cut(s) 1455, 1525
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 1234
BsrDI GCAATG 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 158
BsrI ACTGG 2 cut(s) 1416, 1519
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 4 cut(s) 3, 1047, 1447, 1517
BssNI GRCGYC 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 1322
Bst4CI ACNGT 4 cut(s) 469, 1018, 1223, 1267
Bst6I CTCTTC 1 cut(s) 1491
BstACI GRCGYC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 109, 386, 651
BstDEI CTNAG 7 cut(s) 144, 459, 691, 769, 855, 933, 1191
BstF5I GGATG 2 cut(s) 218, 1294
BstFNI CGCG 1 cut(s) 893
BstKTI GATC 4 cut(s) 6, 1050, 1450, 1520
BstMAI GTCTC 4 cut(s) 258, 674, 694, 1234
BstMBI GATC 4 cut(s) 3, 1047, 1447, 1517
BstMWI GCNNNNNNNGC 4 cut(s) 272, 455, 631, 1151
BstNI CCWGG 1 cut(s) 1322
BstNSI RCATGY 4 cut(s) 159, 388, 493, 1141
BstPAI GACNNNNGTC 1 cut(s) 686
BstSCI CCNGG 1 cut(s) 1320
BstSFI CTRYAG 1 cut(s) 333
BstUI CGCG 1 cut(s) 893
BstV1I GCAGC 5 cut(s) 437, 442, 643, 809, 1072
BstV2I GAAGAC 1 cut(s) 1508
BstX2I RGATCY 2 cut(s) 1447, 1517
BstYI RGATCY 2 cut(s) 1447, 1517
Bsu36I CCTNAGG 1 cut(s) 933
BsuI GTATCC 1 cut(s) 1067
BsuRI GGCC 3 cut(s) 240, 931, 1256
BtgZI GCGATG 1 cut(s) 874
BtsCI GGATG 2 cut(s) 218, 1294
BtsIMutI CAGTG 4 cut(s) 747, 1123, 1219, 1423
Cac8I GCNNGC 3 cut(s) 109, 386, 651
CaiI CAGNNNCTG 1 cut(s) 1385
CciI TCATGA 1 cut(s) 1044
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 4 cut(s) 91, 366, 929, 1196
CseI GACGC 2 cut(s) 240, 974
Csp6I GTAC 6 cut(s) 141, 153, 159, 895, 1014, 1292
CspCI CAANNNNNGTGG 2 cut(s) 11, 46
CviAII CATG 7 cut(s) 156, 385, 490, 646, 1045, 1138, 1258
CviQI GTAC 6 cut(s) 141, 153, 159, 895, 1014, 1292
DdeI CTNAG 7 cut(s) 144, 459, 691, 769, 855, 933, 1191
DpnI GATC 4 cut(s) 5, 1049, 1449, 1519
DpnII GATC 4 cut(s) 3, 1047, 1447, 1517
EaeI YGGCCR 1 cut(s) 1254
Eam1104I CTCTTC 1 cut(s) 1491
EarI CTCTTC 1 cut(s) 1491
EciI GGCGGA 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 1234
Eco47I GGWCC 3 cut(s) 91, 366, 1196
Eco57I CTGAAG 1 cut(s) 1099
Eco81I CCTNAGG 1 cut(s) 933
EcoO109I RGGNCCY 2 cut(s) 929, 1196
EcoRII CCWGG 1 cut(s) 1320
Esp3I CGTCTC 2 cut(s) 258, 674
FaeI CATG 7 cut(s) 159, 388, 493, 649, 1048, 1141, 1261
FaqI GGGAC 2 cut(s) 139, 1510
FatI CATG 7 cut(s) 155, 384, 489, 645, 1044, 1137, 1257
FbaI TGATCA 1 cut(s) 1047
FblI GTMKAC 1 cut(s) 1262
Fnu4HI GCNGC 7 cut(s) 382, 426, 456, 632, 798, 1086, 1383
FokI GGATG 2 cut(s) 225, 1301
Fsp4HI GCNGC 7 cut(s) 382, 426, 456, 632, 798, 1086, 1383
FspBI CTAG 7 cut(s) 389, 608, 881, 1151, 1178, 1233, 1247
GluI GCNGC 7 cut(s) 382, 426, 456, 632, 798, 1086, 1383
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 1 cut(s) 1304
HaeIII GGCC 3 cut(s) 240, 931, 1256
HapII CCGG 1 cut(s) 171
HgaI GACGC 2 cut(s) 240, 974
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 7 cut(s) 159, 388, 493, 649, 1048, 1141, 1261
HindIII AAGCTT 1 cut(s) 1073
HinfI GANTC 4 cut(s) 127, 297, 656, 704
HpaII CCGG 1 cut(s) 171
HphI GGTGA 2 cut(s) 799, 1181
Hpy166II GTNNAC 4 cut(s) 91, 790, 828, 1263
Hpy188I TCNGA 3 cut(s) 258, 462, 694
Hpy188III TCNNGA 5 cut(s) 504, 586, 701, 1045, 1490
Hpy8I GTNNAC 4 cut(s) 91, 790, 828, 1263
HpyAV CCTTC 5 cut(s) 36, 251, 853, 1305, 1487
HpyCH4III ACNGT 4 cut(s) 469, 1018, 1223, 1267
HpyCH4IV ACGT 1 cut(s) 1091
HpyF10VI GCNNNNNNNGC 4 cut(s) 272, 455, 631, 1151
HpyF3I CTNAG 7 cut(s) 144, 459, 691, 769, 855, 933, 1191
HpySE526I ACGT 1 cut(s) 1091
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 7 cut(s) 159, 388, 493, 649, 1048, 1141, 1261
Ksp22I TGATCA 1 cut(s) 1047
Kzo9I GATC 4 cut(s) 3, 1047, 1447, 1517
LmnI GCTCC 2 cut(s) 98, 433
Lsp1109I GCAGC 5 cut(s) 437, 442, 643, 809, 1072
LweI GCATC 2 cut(s) 203, 579
MaeI CTAG 7 cut(s) 389, 608, 881, 1151, 1178, 1233, 1247
MaeII ACGT 1 cut(s) 1091
MaeIII GTNAC 3 cut(s) 48, 667, 884
MalI GATC 4 cut(s) 5, 1049, 1449, 1519
MboI GATC 4 cut(s) 3, 1047, 1447, 1517
MboII GAAGA 6 cut(s) 3, 218, 814, 1448, 1478, 1513
MfeI CAATTG 4 cut(s) 102, 553, 993, 1336
MflI RGATCY 2 cut(s) 1447, 1517
MlsI TGGCCA 1 cut(s) 1256
MluCI AATT 9 cut(s) 82, 102, 199, 553, 816, 993, 1172, 1204, 1336
MluNI TGGCCA 1 cut(s) 1256
MlyI GAGTC 2 cut(s) 121, 291
MmeI TCCRAC 2 cut(s) 289, 1291
Mox20I TGGCCA 1 cut(s) 1256
MroXI GAANNNNTTC 1 cut(s) 547
MscI TGGCCA 1 cut(s) 1256
MseI TTAA 4 cut(s) 72, 636, 873, 1071
Msp20I TGGCCA 1 cut(s) 1256
MspA1I CMGCKG 3 cut(s) 455, 1085, 1382
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 1 cut(s) 1322
MunI CAATTG 4 cut(s) 102, 553, 993, 1336
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 1322
MvnI CGCG 1 cut(s) 893
MwoI GCNNNNNNNGC 4 cut(s) 272, 455, 631, 1151
NdeII GATC 4 cut(s) 3, 1047, 1447, 1517
NlaIII CATG 7 cut(s) 159, 388, 493, 649, 1048, 1141, 1261
NlaIV GGNNCC 3 cut(s) 169, 930, 1197
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 4 cut(s) 159, 388, 493, 1141
PaeI GCATGC 1 cut(s) 388
PagI TCATGA 1 cut(s) 1044
PciI ACATGT 3 cut(s) 155, 489, 1137
PctI GAATGC 1 cut(s) 566
PdmI GAANNNNTTC 1 cut(s) 547
PfeI GAWTC 2 cut(s) 656, 704
PflMI CCANNNNNTGG 1 cut(s) 1385
PfoI TCCNGGA 1 cut(s) 1320
PkrI GCNGC 7 cut(s) 383, 427, 457, 633, 799, 1087, 1384
PleI GAGTC 2 cut(s) 121, 291
PpsI GAGTC 2 cut(s) 121, 291
PpuMI RGGWCCY 1 cut(s) 1196
PscI ACATGT 3 cut(s) 155, 489, 1137
PshAI GACNNNNGTC 1 cut(s) 686
PsiI TTATAA 1 cut(s) 945
Psp5II RGGWCCY 1 cut(s) 1196
Psp6I CCWGG 1 cut(s) 1320
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 1320
PspN4I GGNNCC 3 cut(s) 169, 930, 1197
PspPI GGNCC 4 cut(s) 91, 366, 929, 1196
PspPPI RGGWCCY 1 cut(s) 1196
PstNI CAGNNNCTG 1 cut(s) 1385
PsuI RGATCY 2 cut(s) 1447, 1517
PvuII CAGCTG 2 cut(s) 455, 1085
RsaI GTAC 6 cut(s) 142, 154, 160, 896, 1015, 1293
RsaNI GTAC 6 cut(s) 141, 153, 159, 895, 1014, 1292
SaqAI TTAA 4 cut(s) 72, 636, 873, 1071
SatI GCNGC 7 cut(s) 382, 426, 456, 632, 798, 1086, 1383
Sau3AI GATC 4 cut(s) 3, 1047, 1447, 1517
Sau96I GGNCC 4 cut(s) 91, 366, 929, 1196
SchI GAGTC 2 cut(s) 121, 291
ScrFI CCNGG 1 cut(s) 1322
SfaNI GCATC 2 cut(s) 203, 579
SfcI CTRYAG 1 cut(s) 333
SinI GGWCC 3 cut(s) 91, 366, 1196
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
SpeI ACTAGT 1 cut(s) 1177
SphI GCATGC 1 cut(s) 388
Sse9I AATT 9 cut(s) 82, 102, 199, 553, 816, 993, 1172, 1204, 1336
SsiI CCGC 4 cut(s) 39, 382, 1002, 1382
SspI AATATT 4 cut(s) 483, 761, 1008, 1107
SspMI CTAG 7 cut(s) 389, 608, 881, 1151, 1178, 1233, 1247
StyD4I CCNGG 1 cut(s) 1320
TaaI ACNGT 4 cut(s) 469, 1018, 1223, 1267
TaiI ACGT 1 cut(s) 1094
TaqI TCGA 3 cut(s) 18, 659, 1511
TaqII GACCGA 1 cut(s) 108
TasI AATT 9 cut(s) 82, 102, 199, 553, 816, 993, 1172, 1204, 1336
TatI WGTACW 2 cut(s) 140, 158
TauI GCSGC 2 cut(s) 384, 1385
TfiI GAWTC 2 cut(s) 656, 704
Tru1I TTAA 4 cut(s) 72, 636, 873, 1071
Tru9I TTAA 4 cut(s) 72, 636, 873, 1071
TscAI CASTG 4 cut(s) 747, 1123, 1226, 1423
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 5 cut(s) 425, 455, 631, 797, 1085
Tsp45I GTSAC 1 cut(s) 667
TspGWI ACGGA 1 cut(s) 585
TspRI CASTG 4 cut(s) 747, 1123, 1226, 1423
Van91I CCANNNNNTGG 1 cut(s) 1385
VpaK11BI GGWCC 3 cut(s) 91, 366, 1196
XapI RAATTY 1 cut(s) 816
XceI RCATGY 4 cut(s) 159, 388, 493, 1141
XmiI GTMKAC 1 cut(s) 1262
XmnI GAANNNNTTC 1 cut(s) 547
XspI CTAG 7 cut(s) 389, 608, 881, 1151, 1178, 1233, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.