MD12G1201800.v1.1

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001054
Physical Location & Seq
Forward (+)
28228085 .. 28229668
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1201800.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGGCTCAAAAGGAAGCAAAGGCTTCTGAAGAAGCCAAGCCCCCGTCCCCGGCGGAGACATCCATGATTATTGCTTCAACCGCAGCTCCGGCGGCTCCGGCGGCTCCTTCATGGTTCACGCCCAAGAGGCTACTCGTTATATTTTGCTTGATTAACTTGTTAAACTATGTGGATCGAGGAACAATAGCAAGCAATGGTGTCAATGGGAGCCGCAAAACTTGTACAGAAAGTGGTGTATGCACGCCTGGTACCGGGATACAAGGGGAATTTAACTTGAACAACTTTCAAGATGGGGTTCTGTCATCAGCTTTTATGGCTGGACTTCTTATTGCTTCTCCTATATTTGCATCATTAGCAAAGAGTGTAAATCCATTTAGGCTCATTGGAGTTGGATTATCAGTTTGGACCTTTGCTACACTTGGTTGTGGTTGTTCATTCGGCTTCTGGTCAATTGCTGTTTTCCGCATGCTAGTCGGTGTTGGCGAGGCTTCGTTTATTAGTCTTGCAGCTCCATTCATCGACGACAATGCCCCAGATACTCAGAAAACTGCATGGCTTTCTATATTCTACATGTGCATATCAAGTGGATATGCACTTGGTTATGTTTATGGTGGGGTGGTTGGAAGTCACACCAAGTGGCGCTATGCATTTGTTGGGGAGGCAATCCTTATGCTTCCATTTGCTATTCTAGGGTTTGTTATGAAGCCTTTGCAGATGAAAGGTTTTTCTCATCCTGAATCAAAAAAAGCACCGATAGCCGTGGAAACTGCTGTTGAAGAAGTTCAAGGTTCAGATATTTCGATGAAGGAAGACGTCGGAGATTCTACCATACAAAAGTCTTCCAATTCAAAAGTGAATCCAATTTCAAGGTTTATGAAAGATATGAAGGTGCTTTTGGGGGATAAGGTTTATGTTGTGAATGTTCTAGGTTACATAGCATACAACTTTGTCATAGGTGCATACTCATATTGGGGGCCTAAGGCTGGTTATAACATATATCATATGAGTGATGCAGATATGATATTTGGAGGCGTCACAATTGCGTGCGGGATATTGGGCACACTAGGAGGAGGCTTTCTTCTGGATTATATGACTGGCACCATCTCTAATGCTTTCAAGCTTCTTTCAATGGTAACATTAATTGGGGGTGCCTTTTGCTTAGGTGCCTTCTGGATGAAGAATTTGTATGCTTTCCTAGCTCTCTTCGCAATCGGCCAACTACTCGTCTTTGCAACTCAGGGTCCTGTAAACTACGTCTGTCTCCATTGTGTTAAACCTAGCATGAGGCCACTATCTATGGCGATTTCTACGGTTGCAATTCACCTTTTCGGGGATGTACCTTCCGCGCCTCTTGTCGGAGTTCTCCAGGATGCCATTAACGACTGGAGGGCGACTGCTCTTATTCTAACATCTATTTTCTTTCCAGCAGCTCTAATATGGTTTATAGGAATCTTTCTGCACAGCGTGGATAGATTTAACGAAGAAAGTGAGCATCAGATCACCACCAAAACCGAAACGTCAAACACAACACCATTGGATGAAGGGAAGAAGATAGAAACAATTGAATCGAACCATAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

528

Amino Acids

56.69

Weight (kDa)

6.46

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 85 - 441 3.4e-31 Major Facilitator Superfamily
OATP PF03137 153 - 392 6.2e-20 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 393 - 457 2.7e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 990
AatII GACGTC 1 cut(s) 816
Acc65I GGTACC 1 cut(s) 248
AccB1I GGYRCC 4 cut(s) 248, 1097, 1148, 1163
AccII CGCG 1 cut(s) 1346
AciI CCGC 8 cut(s) 53, 81, 92, 101, 211, 463, 1047, 1344
AclWI GGATC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 1213
AcsI RAATTY 2 cut(s) 266, 1180
AcuI CTGAAG 1 cut(s) 48
AcyI GRCGYC 2 cut(s) 813, 1032
AdeI CACNNNGTG 2 cut(s) 636, 1465
AfaI GTAC 3 cut(s) 223, 250, 1338
AfiI CCNNNNNNNGG 5 cut(s) 49, 251, 983, 1330, 1355
AflIII ACRYGT 1 cut(s) 570
AjnI CCWGG 2 cut(s) 244, 1365
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
AluBI AGCT 6 cut(s) 86, 308, 509, 1120, 1199, 1430
AluI AGCT 6 cut(s) 86, 308, 509, 1120, 1199, 1430
Alw26I GTCTC 2 cut(s) 50, 1265
AlwI GGATC 1 cut(s) 180
AoxI GGCC 3 cut(s) 974, 1213, 1286
ApeKI GCWGC 3 cut(s) 83, 506, 1427
ApoI RAATTY 2 cut(s) 266, 1180
AseI ATTAAT 1 cut(s) 1139
Asp700I GAANNNNTTC 1 cut(s) 780
Asp718I GGTACC 1 cut(s) 248
AspLEI GCGC 2 cut(s) 642, 1348
AspS9I GGNCC 3 cut(s) 405, 974, 1241
AsuC2I CCSGG 2 cut(s) 50, 253
AsuHPI GGTGA 2 cut(s) 1313, 1492
AvaII GGWCC 2 cut(s) 405, 1241
AxyI CCTNAGG 1 cut(s) 978
BaeGI GKGCMC 1 cut(s) 1061
BanI GGYRCC 4 cut(s) 248, 1097, 1148, 1163
BbsI GAAGAC 2 cut(s) 816, 831
BbvI GCAGC 3 cut(s) 95, 518, 1439
BccI CCATC 2 cut(s) 284, 1109
BceAI ACGGC 1 cut(s) 743
BcgI CGANNNNNNTGC 4 cut(s) 454, 488, 509, 543
BciT130I CCWGG 2 cut(s) 246, 1367
BciVI GTATCC 1 cut(s) 249
BcnI CCSGG 2 cut(s) 50, 253
BcoDI GTCTC 2 cut(s) 50, 1265
BfaI CTAG 6 cut(s) 470, 689, 926, 1064, 1196, 1278
BfoI RGCGCY 1 cut(s) 643
BfuI GTATCC 1 cut(s) 249
BglI GCCNNNNNGGC 1 cut(s) 127
BisI GCNGC 6 cut(s) 84, 93, 102, 211, 507, 1428
BlsI GCNGC 6 cut(s) 85, 94, 103, 212, 508, 1429
Bme1390I CCNGG 4 cut(s) 50, 246, 253, 1367
Bme18I GGWCC 2 cut(s) 405, 1241
BmgT120I GGNCC 3 cut(s) 405, 974, 1241
BmiI GGNNCC 9 cut(s) 96, 105, 209, 250, 975, 1099, 1150, 1165, 1242
BmrFI CCNGG 4 cut(s) 50, 246, 253, 1367
BmsI GCATC 4 cut(s) 356, 1000, 1360, 1501
BpiI GAAGAC 2 cut(s) 816, 831
BpmI CTGGAG 2 cut(s) 1349, 1405
Bpu10I CCTNAGC 1 cut(s) 1159
BpuMI CCSGG 2 cut(s) 50, 253
BsaHI GRCGYC 2 cut(s) 813, 1032
BsaJI CCNNGG 2 cut(s) 48, 759
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 251, 983, 1330, 1355
Bse1I ACTGG 2 cut(s) 1099, 1388
Bse21I CCTNAGG 1 cut(s) 978
Bse3DI GCAATG 1 cut(s) 199
BseBI CCWGG 2 cut(s) 246, 1367
BseDI CCNNGG 2 cut(s) 48, 759
BseGI GGATG 6 cut(s) 59, 730, 1179, 1339, 1375, 1543
BseLI CCNNNNNNNGG 5 cut(s) 49, 251, 983, 1330, 1355
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 554, 1250
BseNI ACTGG 2 cut(s) 1099, 1388
BseRI GAGGAG 1 cut(s) 1083
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 3 cut(s) 95, 518, 1439
BsgI GTGCAG 1 cut(s) 1442
Bsh1236I CGCG 1 cut(s) 1346
BshFI GGCC 3 cut(s) 976, 1215, 1288
BshNI GGYRCC 4 cut(s) 248, 1097, 1148, 1163
BsiSI CCGG 4 cut(s) 50, 89, 98, 252
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 5 cut(s) 49, 251, 983, 1330, 1355
BsmAI GTCTC 2 cut(s) 50, 1265
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 3 cut(s) 976, 1215, 1288
Bsp1286I GDGCHC 1 cut(s) 1061
Bsp1407I TGTACA 1 cut(s) 221
Bsp143I GATC 2 cut(s) 172, 1497
BspACI CCGC 8 cut(s) 53, 81, 92, 101, 211, 463, 1047, 1344
BspANI GGCC 3 cut(s) 976, 1215, 1288
BspCNI CTCAG 2 cut(s) 553, 1249
BspFNI CGCG 1 cut(s) 1346
BspLI GGNNCC 9 cut(s) 96, 105, 209, 250, 975, 1099, 1150, 1165, 1242
BspPI GGATC 1 cut(s) 180
BspT107I GGYRCC 4 cut(s) 248, 1097, 1148, 1163
BsrDI GCAATG 1 cut(s) 199
BsrGI TGTACA 1 cut(s) 221
BsrI ACTGG 2 cut(s) 1099, 1388
BssECI CCNNGG 2 cut(s) 48, 759
BssMI GATC 2 cut(s) 172, 1497
BssNI GRCGYC 2 cut(s) 813, 1032
Bst2UI CCWGG 2 cut(s) 246, 1367
Bst4CI ACNGT 1 cut(s) 1312
Bst6I CTCTTC 1 cut(s) 1208
BstACI GRCGYC 2 cut(s) 813, 1032
BstAUI TGTACA 1 cut(s) 221
BstC8I GCNNGC 4 cut(s) 190, 242, 467, 1045
BstDEI CTNAG 4 cut(s) 540, 978, 1159, 1236
BstDSI CCRYGG 1 cut(s) 759
BstF5I GGATG 6 cut(s) 59, 730, 1179, 1339, 1375, 1543
BstFNI CGCG 1 cut(s) 1346
BstH2I RGCGCY 1 cut(s) 643
BstHHI GCGC 2 cut(s) 642, 1348
BstKTI GATC 2 cut(s) 175, 1500
BstMAI GTCTC 2 cut(s) 50, 1265
BstMBI GATC 2 cut(s) 172, 1497
BstNI CCWGG 2 cut(s) 246, 1367
BstNSI RCATGY 2 cut(s) 469, 574
BstSCI CCNGG 4 cut(s) 48, 244, 251, 1365
BstSLI GKGCMC 1 cut(s) 1061
BstUI CGCG 1 cut(s) 1346
BstV1I GCAGC 3 cut(s) 95, 518, 1439
BstV2I GAAGAC 2 cut(s) 816, 831
Bsu36I CCTNAGG 1 cut(s) 978
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 3 cut(s) 976, 1215, 1288
BtgI CCRYGG 1 cut(s) 759
BtsCI GGATG 6 cut(s) 59, 730, 1179, 1339, 1375, 1543
Cac8I GCNNGC 4 cut(s) 190, 242, 467, 1045
CfoI GCGC 2 cut(s) 642, 1348
Cfr13I GGNCC 3 cut(s) 405, 974, 1241
CseI GACGC 1 cut(s) 1021
Csp6I GTAC 3 cut(s) 222, 249, 1337
CviAII CATG 6 cut(s) 64, 111, 466, 552, 571, 1282
CviQI GTAC 3 cut(s) 222, 249, 1337
DdeI CTNAG 4 cut(s) 540, 978, 1159, 1236
DpnI GATC 2 cut(s) 174, 1499
DpnII GATC 2 cut(s) 172, 1497
DraIII CACNNNGTG 2 cut(s) 636, 1465
EaeI YGGCCR 1 cut(s) 1213
Eam1104I CTCTTC 1 cut(s) 1208
EarI CTCTTC 1 cut(s) 1208
EciI GGCGGA 1 cut(s) 68
Eco47I GGWCC 2 cut(s) 405, 1241
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 978
EcoO109I RGGNCCY 2 cut(s) 974, 1241
EcoRII CCWGG 2 cut(s) 244, 1365
EcoT22I ATGCAT 1 cut(s) 649
FaeI CATG 6 cut(s) 67, 114, 469, 555, 574, 1285
FaqI GGGAC 1 cut(s) 31
FatI CATG 6 cut(s) 63, 110, 465, 551, 570, 1281
FauI CCCGC 1 cut(s) 1040
FauNDI CATATG 1 cut(s) 1002
Fnu4HI GCNGC 6 cut(s) 84, 93, 102, 211, 507, 1428
FokI GGATG 6 cut(s) 46, 717, 1186, 1346, 1382, 1550
Fsp4HI GCNGC 6 cut(s) 84, 93, 102, 211, 507, 1428
FspBI CTAG 6 cut(s) 470, 689, 926, 1064, 1196, 1278
GlaI GCGC 2 cut(s) 641, 1347
GluI GCNGC 6 cut(s) 84, 93, 102, 211, 507, 1428
GsuI CTGGAG 2 cut(s) 1349, 1405
HaeII RGCGCY 1 cut(s) 643
HaeIII GGCC 3 cut(s) 976, 1215, 1288
HapII CCGG 4 cut(s) 50, 89, 98, 252
HgaI GACGC 1 cut(s) 1021
HhaI GCGC 2 cut(s) 642, 1348
Hin1I GRCGYC 2 cut(s) 813, 1032
Hin1II CATG 6 cut(s) 67, 114, 469, 555, 574, 1285
Hin6I GCGC 2 cut(s) 640, 1346
HinP1I GCGC 2 cut(s) 640, 1346
HindIII AAGCTT 1 cut(s) 1118
HinfI GANTC 5 cut(s) 737, 821, 856, 1449, 1565
HpaII CCGG 4 cut(s) 50, 89, 98, 252
HphI GGTGA 2 cut(s) 1313, 1492
Hpy166II GTNNAC 2 cut(s) 117, 1249
Hpy188I TCNGA 6 cut(s) 28, 543, 793, 818, 1358, 1497
Hpy188III TCNNGA 4 cut(s) 287, 734, 1082, 1171
Hpy8I GTNNAC 2 cut(s) 117, 1249
Hpy99I CGWCG 2 cut(s) 524, 818
HpyAV CCTTC 6 cut(s) 117, 799, 880, 1177, 1350, 1535
HpyCH4III ACNGT 1 cut(s) 1312
HpyCH4IV ACGT 3 cut(s) 813, 1254, 1517
HpyF3I CTNAG 4 cut(s) 540, 978, 1159, 1236
HpySE526I ACGT 3 cut(s) 813, 1254, 1517
Hsp92I GRCGYC 2 cut(s) 813, 1032
Hsp92II CATG 6 cut(s) 67, 114, 469, 555, 574, 1285
HspAI GCGC 2 cut(s) 640, 1346
KpnI GGTACC 1 cut(s) 252
Kzo9I GATC 2 cut(s) 172, 1497
LmnI GCTCC 5 cut(s) 91, 100, 109, 207, 514
Lsp1109I GCAGC 3 cut(s) 95, 518, 1439
LweI GCATC 4 cut(s) 356, 1000, 1360, 1501
MaeI CTAG 6 cut(s) 470, 689, 926, 1064, 1196, 1278
MaeII ACGT 3 cut(s) 813, 1254, 1517
MaeIII GTNAC 4 cut(s) 626, 929, 1033, 1132
MalI GATC 2 cut(s) 174, 1499
MboI GATC 2 cut(s) 172, 1497
MfeI CAATTG 3 cut(s) 450, 1038, 1560
MhlI GDGCHC 1 cut(s) 1061
MluCI AATT 9 cut(s) 266, 450, 844, 861, 1038, 1140, 1180, 1317, 1560
MmeI TCCRAC 4 cut(s) 370, 601, 796, 1336
Mph1103I ATGCAT 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 780
MseI TTAA 7 cut(s) 153, 161, 270, 1139, 1272, 1377, 1476
MslI CAYNNNNRTG 1 cut(s) 1005
MspI CCGG 4 cut(s) 50, 89, 98, 252
MspR9I CCNGG 4 cut(s) 50, 246, 253, 1367
MunI CAATTG 3 cut(s) 450, 1038, 1560
MvaI CCWGG 2 cut(s) 246, 1367
MvnI CGCG 1 cut(s) 1346
NciI CCSGG 2 cut(s) 50, 253
NdeI CATATG 1 cut(s) 1002
NdeII GATC 2 cut(s) 172, 1497
NlaIII CATG 6 cut(s) 67, 114, 469, 555, 574, 1285
NlaIV GGNNCC 9 cut(s) 96, 105, 209, 250, 975, 1099, 1150, 1165, 1242
NmuCI GTSAC 2 cut(s) 626, 1033
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 2 cut(s) 469, 574
PaeI GCATGC 1 cut(s) 469
PciI ACATGT 1 cut(s) 570
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 5 cut(s) 737, 821, 856, 1449, 1565
PfoI TCCNGGA 1 cut(s) 1365
PkrI GCNGC 6 cut(s) 85, 94, 103, 212, 508, 1429
PpuMI RGGWCCY 1 cut(s) 1241
PscI ACATGT 1 cut(s) 570
PshBI ATTAAT 1 cut(s) 1139
PsiI TTATAA 1 cut(s) 990
Psp5II RGGWCCY 1 cut(s) 1241
Psp6I CCWGG 2 cut(s) 244, 1365
PspGI CCWGG 2 cut(s) 244, 1365
PspN4I GGNNCC 9 cut(s) 96, 105, 209, 250, 975, 1099, 1150, 1165, 1242
PspPI GGNCC 3 cut(s) 405, 974, 1241
PspPPI RGGWCCY 1 cut(s) 1241
RsaI GTAC 3 cut(s) 223, 250, 1338
RsaNI GTAC 3 cut(s) 222, 249, 1337
RseI CAYNNNNRTG 1 cut(s) 1005
SaqAI TTAA 7 cut(s) 153, 161, 270, 1139, 1272, 1377, 1476
SatI GCNGC 6 cut(s) 84, 93, 102, 211, 507, 1428
Sau3AI GATC 2 cut(s) 172, 1497
Sau96I GGNCC 3 cut(s) 405, 974, 1241
ScrFI CCNGG 4 cut(s) 50, 246, 253, 1367
SduI GDGCHC 1 cut(s) 1061
SfaNI GCATC 4 cut(s) 356, 1000, 1360, 1501
SinI GGWCC 2 cut(s) 405, 1241
SmiMI CAYNNNNRTG 1 cut(s) 1005
SphI GCATGC 1 cut(s) 469
Sse9I AATT 9 cut(s) 266, 450, 844, 861, 1038, 1140, 1180, 1317, 1560
SsiI CCGC 8 cut(s) 53, 81, 92, 101, 211, 463, 1047, 1344
SspMI CTAG 6 cut(s) 470, 689, 926, 1064, 1196, 1278
StyD4I CCNGG 4 cut(s) 48, 244, 251, 1365
TaaI ACNGT 1 cut(s) 1312
TaiI ACGT 3 cut(s) 816, 1257, 1520
TaqI TCGA 4 cut(s) 175, 519, 800, 1568
TasI AATT 9 cut(s) 266, 450, 844, 861, 1038, 1140, 1180, 1317, 1560
TatI WGTACW 1 cut(s) 221
TauI GCSGC 3 cut(s) 95, 104, 213
TfiI GAWTC 5 cut(s) 737, 821, 856, 1449, 1565
Tru1I TTAA 7 cut(s) 153, 161, 270, 1139, 1272, 1377, 1476
Tru9I TTAA 7 cut(s) 153, 161, 270, 1139, 1272, 1377, 1476
TseFI GTSAC 2 cut(s) 626, 1033
TseI GCWGC 3 cut(s) 83, 506, 1427
Tsp45I GTSAC 2 cut(s) 626, 1033
VpaK11BI GGWCC 2 cut(s) 405, 1241
VspI ATTAAT 1 cut(s) 1139
XapI RAATTY 2 cut(s) 266, 1180
XceI RCATGY 2 cut(s) 469, 574
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 6 cut(s) 470, 689, 926, 1064, 1196, 1278
ZraI GACGTC 1 cut(s) 814
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.