Rmu_sc0004362.1_g000010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004362.1
OG0001054
Physical Location & Seq
Reverse (-)
26963 .. 31115
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004362.1_g000010.1.cds

Sequence Viewer

Length: 1146 bp
atgataaaagccagcctgccgccgatcgaggcggggttcaaattgctgacggagaacgtgaagaatgtggagaagctagctggaaatggagtagagggttgcttgaagcggacgggctttgagtacagcactctgccggcgccggagttggggacggcaaggtcgcgagtgagacgcacgctgccattgttcaagtgggcgtcgccgatgagcttgaggctgctgagggtgacggttccgaagtcgaattcggttacggctgacgtaatgaggaagttaattggaaaagcaaaggtaggttgcgcaaaggtttgctgcagagcacgggggctgaccggcttggctgctgggcaggaggcctgtgaaggccagtcggtgatgcaggcgctggggccggaggttgggctgttgggcaaggtgttgcaagtaggtggtggccgcgcagttgttgaggccgcttggctgcagggaggcaaggccgagcgcgcggggtcgttgtcaggtggggaggcgctggggtgtaggctcgcaggcggctgggctgtgtgtgcagccctgggcaaggctgaggcagatttgagacttgatggccatgagcagaggcttcttgggattggagccgtcgtattgcttgagctcctcggtggtgatctcacagagctagaccggcggcggaagaggctgcatctcttccaagctcctggggcggttgtgggtctcggacggcccaaaaagcccagaaaggacatgtggccaaacctgcgcaatcttgtagcaaaggaacacatgaatctgataaaactccaccgttccggcgagttgatgactcgtctagaacctgctcctcgaaaatccaaactcggcatcgctattgctacttccgctggtgtccaccaacaaaccctcgttctaagttggagaacgagtcgagagatggtgccggcgattttgttgaaggtggatagtgggtgcgattggttcatacttaatcttataaatggattcattccagttaaggtgagctgcgtttcgagtaaggaacaagaggagaggatgaagaaaaaagagaagaataaaaaaaatgtaaaaggggaggtcagggctattctcgacattttaccatccaccatgcttacatggtgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

381

Amino Acids

41.18

Weight (kDa)

10.01

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 995
AasI GACNNNNNNGTC 1 cut(s) 160
Acc16I TGCGCA 2 cut(s) 304, 764
Acc36I ACCTGC 2 cut(s) 768, 847
AccB1I GGYRCC 2 cut(s) 139, 937
AccII CGCG 4 cut(s) 166, 441, 486, 488
AcoI YGGCCR 3 cut(s) 436, 589, 752
AcsI RAATTY 1 cut(s) 247
AcyI GRCGYC 2 cut(s) 140, 200
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 149, 401, 562
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 4 cut(s) 40, 106, 193, 955
AjnI CCWGG 2 cut(s) 555, 700
AluBI AGCT 7 cut(s) 76, 80, 213, 637, 661, 698, 1023
AluI AGCT 7 cut(s) 76, 80, 213, 637, 661, 698, 1023
Alw21I GWGCWC 2 cut(s) 325, 639
Alw26I GTCTC 3 cut(s) 166, 574, 722
AlwNI CAGNNNCTG 1 cut(s) 388
AoxI GGCC 9 cut(s) 357, 367, 392, 436, 453, 477, 589, 725, 752
ApeKI GCWGC 8 cut(s) 181, 220, 315, 344, 463, 551, 682, 1023
ApoI RAATTY 1 cut(s) 247
AspLEI GCGC 8 cut(s) 142, 305, 388, 443, 486, 488, 514, 765
AspS9I GGNCC 2 cut(s) 392, 726
AsuHPI GGTGA 4 cut(s) 241, 388, 659, 1030
AsuNHI GCTAGC 1 cut(s) 76
BalI TGGCCA 2 cut(s) 591, 754
BanI GGYRCC 2 cut(s) 139, 937
BanII GRGCYC 1 cut(s) 639
Bbv12I GWGCWC 2 cut(s) 325, 639
BbvCI CCTCAGC 2 cut(s) 224, 567
BbvI GCAGC 8 cut(s) 168, 207, 302, 331, 450, 563, 669, 1010
BccI CCATC 3 cut(s) 581, 928, 1129
BceAI ACGGC 4 cut(s) 171, 273, 605, 740
BciT130I CCWGG 2 cut(s) 557, 702
BcoDI GTCTC 3 cut(s) 166, 574, 722
BfaI CTAG 3 cut(s) 77, 662, 833
BfmI CTRYAG 2 cut(s) 316, 464
BfoI RGCGCY 3 cut(s) 143, 389, 515
BfuAI ACCTGC 2 cut(s) 768, 847
Bme1390I CCNGG 2 cut(s) 557, 702
BmgT120I GGNCC 2 cut(s) 392, 726
BmiI GGNNCC 5 cut(s) 141, 237, 393, 619, 939
BmrFI CCNGG 2 cut(s) 557, 702
BmsI GCATC 3 cut(s) 369, 694, 873
BmtI GCTAGC 1 cut(s) 80
Bpu10I CCTNAGC 2 cut(s) 224, 567
BpuEI CTTGAG 2 cut(s) 235, 653
BsaHI GRCGYC 2 cut(s) 140, 200
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 4 cut(s) 555, 556, 640, 701
BsaXI ACNNNNNCTCC 4 cut(s) 609, 639, 910, 940
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 401, 562
Bse118I RCCGGY 4 cut(s) 136, 335, 666, 940
Bse1I ACTGG 2 cut(s) 370, 1010
BseBI CCWGG 2 cut(s) 557, 702
BseDI CCNNGG 4 cut(s) 555, 556, 640, 701
BseGI GGATG 2 cut(s) 1059, 1121
BseLI CCNNNNNNNGG 3 cut(s) 149, 401, 562
BseMII CTCAG 2 cut(s) 215, 558
BseNI ACTGG 2 cut(s) 370, 1010
BsePI GCGCGC 1 cut(s) 484
BseRI GAGGAG 3 cut(s) 629, 834, 1061
BseXI GCAGC 8 cut(s) 168, 207, 302, 331, 450, 563, 669, 1010
BseYI CCCAGC 4 cut(s) 347, 388, 514, 537
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 4 cut(s) 166, 441, 486, 488
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 9 cut(s) 359, 369, 394, 438, 455, 479, 591, 727, 754
BshNI GGYRCC 2 cut(s) 139, 937
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 325, 639
BsiSI CCGG 7 cut(s) 137, 143, 336, 395, 667, 813, 941
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 3 cut(s) 149, 401, 562
BsmAI GTCTC 3 cut(s) 166, 574, 722
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 9 cut(s) 359, 369, 394, 438, 455, 479, 591, 727, 754
Bso31I GGTCTC 1 cut(s) 722
Bsp1286I GDGCHC 2 cut(s) 325, 639
Bsp143I GATC 2 cut(s) 24, 649
Bsp68I TCGCGA 1 cut(s) 166
BspANI GGCC 9 cut(s) 359, 369, 394, 438, 455, 479, 591, 727, 754
BspCNI CTCAG 2 cut(s) 216, 559
BspFNI CGCG 4 cut(s) 166, 441, 486, 488
BspLI GGNNCC 5 cut(s) 141, 237, 393, 619, 939
BspMAI CTGCAG 2 cut(s) 320, 468
BspMI ACCTGC 2 cut(s) 768, 847
BspOI GCTAGC 1 cut(s) 80
BspT107I GGYRCC 2 cut(s) 139, 937
BspTNI GGTCTC 1 cut(s) 722
BsrFI RCCGGY 4 cut(s) 136, 335, 666, 940
BsrI ACTGG 2 cut(s) 370, 1010
BssAI RCCGGY 4 cut(s) 136, 335, 666, 940
BssECI CCNNGG 4 cut(s) 555, 556, 640, 701
BssHII GCGCGC 1 cut(s) 484
BssMI GATC 2 cut(s) 24, 649
BssNI GRCGYC 2 cut(s) 140, 200
Bst2UI CCWGG 2 cut(s) 557, 702
Bst4CI ACNGT 2 cut(s) 235, 809
Bst6I CTCTTC 2 cut(s) 671, 695
BstACI GRCGYC 2 cut(s) 140, 200
BstDEI CTNAG 3 cut(s) 224, 567, 911
BstENI CCTNNNNNAGG 1 cut(s) 560
BstF5I GGATG 2 cut(s) 1059, 1121
BstFNI CGCG 4 cut(s) 166, 441, 486, 488
BstH2I RGCGCY 3 cut(s) 143, 389, 515
BstHHI GCGC 8 cut(s) 142, 305, 388, 443, 486, 488, 514, 765
BstKTI GATC 2 cut(s) 27, 652
BstMAI GTCTC 3 cut(s) 166, 574, 722
BstMBI GATC 2 cut(s) 24, 649
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 8 cut(s) 485, 548, 667, 679, 704, 733, 760, 881
BstNI CCWGG 2 cut(s) 557, 702
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 2 cut(s) 555, 700
BstSFI CTRYAG 2 cut(s) 316, 464
BstUI CGCG 4 cut(s) 166, 441, 486, 488
BstV1I GCAGC 8 cut(s) 168, 207, 302, 331, 450, 563, 669, 1010
BstXI CCANNNNNNTGG 1 cut(s) 701
BsuRI GGCC 9 cut(s) 359, 369, 394, 438, 455, 479, 591, 727, 754
BtgZI GCGATG 1 cut(s) 850
BtsCI GGATG 2 cut(s) 1059, 1121
BtuMI TCGCGA 1 cut(s) 166
BveI ACCTGC 2 cut(s) 768, 847
CaiI CAGNNNCTG 1 cut(s) 388
CfoI GCGC 8 cut(s) 142, 305, 388, 443, 486, 488, 514, 765
Cfr10I RCCGGY 4 cut(s) 136, 335, 666, 940
Cfr13I GGNCC 2 cut(s) 392, 726
CseI GACGC 2 cut(s) 183, 189
Csp6I GTAC 1 cut(s) 124
CviAII CATG 5 cut(s) 593, 748, 787, 1129, 1137
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 3 cut(s) 224, 567, 911
DinI GGCGCC 1 cut(s) 141
DpnI GATC 2 cut(s) 26, 651
DpnII GATC 2 cut(s) 24, 649
DrdI GACNNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 160
EaeI YGGCCR 3 cut(s) 436, 589, 752
Eam1104I CTCTTC 2 cut(s) 671, 695
EarI CTCTTC 2 cut(s) 671, 695
EciI GGCGGA 1 cut(s) 688
Ecl136II GAGCTC 1 cut(s) 637
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 639
Eco31I GGTCTC 1 cut(s) 722
Eco53kI GAGCTC 1 cut(s) 637
EcoICRI GAGCTC 1 cut(s) 637
EcoNI CCTNNNNNAGG 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 2 cut(s) 555, 700
EcoT38I GRGCYC 1 cut(s) 639
EgeI GGCGCC 1 cut(s) 141
EheI GGCGCC 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 5 cut(s) 596, 751, 790, 1132, 1140
FaiI YATR 7 cut(s) 594, 749, 788, 983, 995, 1130, 1138
FaqI GGGAC 1 cut(s) 166
FatI CATG 5 cut(s) 592, 747, 786, 1128, 1136
FauI CCCGC 2 cut(s) 25, 481
FokI GGATG 2 cut(s) 1066, 1108
FriOI GRGCYC 1 cut(s) 639
FspBI CTAG 3 cut(s) 77, 662, 833
FspI TGCGCA 2 cut(s) 304, 764
GlaI GCGC 8 cut(s) 141, 304, 387, 442, 485, 487, 513, 764
GsaI CCCAGC 4 cut(s) 351, 392, 518, 541
HaeII RGCGCY 3 cut(s) 143, 389, 515
HaeIII GGCC 9 cut(s) 359, 369, 394, 438, 455, 479, 591, 727, 754
HapII CCGG 7 cut(s) 137, 143, 336, 395, 667, 813, 941
HgaI GACGC 2 cut(s) 183, 189
HhaI GCGC 8 cut(s) 142, 305, 388, 443, 486, 488, 514, 765
Hin1I GRCGYC 2 cut(s) 140, 200
Hin1II CATG 5 cut(s) 596, 751, 790, 1132, 1140
Hin6I GCGC 8 cut(s) 140, 303, 386, 441, 484, 486, 512, 763
HinP1I GCGC 8 cut(s) 140, 303, 386, 441, 484, 486, 512, 763
HinfI GANTC 4 cut(s) 790, 826, 925, 1002
HpaII CCGG 7 cut(s) 137, 143, 336, 395, 667, 813, 941
HphI GGTGA 4 cut(s) 241, 388, 659, 1030
Hpy166II GTNNAC 1 cut(s) 892
Hpy188I TCNGA 3 cut(s) 240, 722, 795
Hpy188III TCNNGA 4 cut(s) 165, 833, 929, 1109
Hpy8I GTNNAC 1 cut(s) 892
Hpy99I CGWCG 2 cut(s) 205, 626
HpyAV CCTTC 2 cut(s) 359, 949
HpyCH4III ACNGT 2 cut(s) 235, 809
HpyCH4IV ACGT 2 cut(s) 57, 264
HpyCH4V TGCA 6 cut(s) 318, 382, 424, 466, 551, 685
HpyF10VI GCNNNNNNNGC 8 cut(s) 485, 548, 667, 679, 704, 733, 760, 881
HpyF3I CTNAG 3 cut(s) 224, 567, 911
HpySE526I ACGT 2 cut(s) 57, 264
Hsp92I GRCGYC 2 cut(s) 140, 200
Hsp92II CATG 5 cut(s) 596, 751, 790, 1132, 1140
HspAI GCGC 8 cut(s) 140, 303, 386, 441, 484, 486, 512, 763
KasI GGCGCC 1 cut(s) 139
KroI GCCGGC 2 cut(s) 136, 940
KroNI GCCGGC 2 cut(s) 138, 942
Kzo9I GATC 2 cut(s) 24, 649
LmnI GCTCC 4 cut(s) 617, 642, 703, 847
Lsp1109I GCAGC 8 cut(s) 168, 207, 302, 331, 450, 563, 669, 1010
LweI GCATC 3 cut(s) 369, 694, 873
MaeI CTAG 3 cut(s) 77, 662, 833
MaeII ACGT 2 cut(s) 57, 264
MaeIII GTNAC 2 cut(s) 229, 253
MalI GATC 2 cut(s) 26, 651
MboI GATC 2 cut(s) 24, 649
MboII GAAGA 5 cut(s) 73, 682, 688, 1069, 1081
MhlI GDGCHC 2 cut(s) 325, 639
MlsI TGGCCA 2 cut(s) 591, 754
MluCI AATT 3 cut(s) 41, 247, 279
MluNI TGGCCA 2 cut(s) 591, 754
Mly113I GGCGCC 1 cut(s) 140
MlyI GAGTC 2 cut(s) 820, 934
MmeI TCCRAC 1 cut(s) 896
Mox20I TGGCCA 2 cut(s) 591, 754
MroNI GCCGGC 2 cut(s) 136, 940
MscI TGGCCA 2 cut(s) 591, 754
MseI TTAA 3 cut(s) 278, 987, 1014
Msp20I TGGCCA 2 cut(s) 591, 754
MspA1I CMGCKG 1 cut(s) 884
MspI CCGG 7 cut(s) 137, 143, 336, 395, 667, 813, 941
MspR9I CCNGG 2 cut(s) 557, 702
MvaI CCWGG 2 cut(s) 557, 702
MvnI CGCG 4 cut(s) 166, 441, 486, 488
MwoI GCNNNNNNNGC 8 cut(s) 485, 548, 667, 679, 704, 733, 760, 881
NaeI GCCGGC 2 cut(s) 138, 942
NarI GGCGCC 1 cut(s) 140
NdeII GATC 2 cut(s) 24, 649
NgoMIV GCCGGC 2 cut(s) 136, 940
NheI GCTAGC 1 cut(s) 76
NlaIII CATG 5 cut(s) 596, 751, 790, 1132, 1140
NlaIV GGNNCC 5 cut(s) 141, 237, 393, 619, 939
NmeAIII GCCGAG 2 cut(s) 505, 840
NmuCI GTSAC 1 cut(s) 229
NruI TCGCGA 1 cut(s) 166
NsbI TGCGCA 2 cut(s) 304, 764
NspI RCATGY 1 cut(s) 751
PasI CCCWGGG 1 cut(s) 556
PauI GCGCGC 1 cut(s) 484
PceI AGGCCT 1 cut(s) 359
PciI ACATGT 1 cut(s) 747
PdiI GCCGGC 2 cut(s) 138, 942
PfeI GAWTC 2 cut(s) 790, 1002
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 2 cut(s) 820, 933
PluTI GGCGCC 1 cut(s) 143
PpsI GAGTC 2 cut(s) 820, 933
PscI ACATGT 1 cut(s) 747
PsiI TTATAA 1 cut(s) 995
Psp124BI GAGCTC 1 cut(s) 639
Psp6I CCWGG 2 cut(s) 555, 700
PspFI CCCAGC 4 cut(s) 347, 388, 514, 537
PspGI CCWGG 2 cut(s) 555, 700
PspN4I GGNNCC 5 cut(s) 141, 237, 393, 619, 939
PspPI GGNCC 2 cut(s) 392, 726
PstI CTGCAG 2 cut(s) 320, 468
PstNI CAGNNNCTG 1 cut(s) 388
PteI GCGCGC 1 cut(s) 484
PvuI CGATCG 1 cut(s) 27
RruI TCGCGA 1 cut(s) 166
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 639
SaqAI TTAA 3 cut(s) 278, 987, 1014
Sau3AI GATC 2 cut(s) 24, 649
Sau96I GGNCC 2 cut(s) 392, 726
SchI GAGTC 2 cut(s) 820, 934
ScrFI CCNGG 2 cut(s) 557, 702
SduI GDGCHC 2 cut(s) 325, 639
SfaNI GCATC 3 cut(s) 369, 694, 873
SfcI CTRYAG 2 cut(s) 316, 464
SfoI GGCGCC 1 cut(s) 141
SmlI CTYRAG 2 cut(s) 214, 632
SmoI CTYRAG 2 cut(s) 214, 632
Sse9I AATT 3 cut(s) 41, 247, 279
SseBI AGGCCT 1 cut(s) 359
SspDI GGCGCC 1 cut(s) 139
SspMI CTAG 3 cut(s) 77, 662, 833
SstI GAGCTC 1 cut(s) 639
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 555, 700
TaaI ACNGT 2 cut(s) 235, 809
TaiI ACGT 2 cut(s) 60, 267
TaqI TCGA 6 cut(s) 27, 245, 847, 928, 1031, 1110
TasI AATT 3 cut(s) 41, 247, 279
TatI WGTACW 1 cut(s) 123
TauI GCSGC 5 cut(s) 22, 441, 458, 537, 673
TfiI GAWTC 2 cut(s) 790, 1002
Tru1I TTAA 3 cut(s) 278, 987, 1014
Tru9I TTAA 3 cut(s) 278, 987, 1014
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 8 cut(s) 181, 220, 315, 344, 463, 551, 682, 1023
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 4 cut(s) 803, 970, 994, 1070
TspGWI ACGGA 1 cut(s) 65
XagI CCTNNNNNAGG 1 cut(s) 560
XapI RAATTY 1 cut(s) 247
XbaI TCTAGA 1 cut(s) 832
XceI RCATGY 1 cut(s) 751
XcmI CCANNNNNNNNNTGG 1 cut(s) 192
XspI CTAG 3 cut(s) 77, 662, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.