pycom10g04500

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001054
Physical Location & Seq
Forward (+)
4712985 .. 4726638
13654 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04500.15

Sequence Viewer

Length: 1629 bp
ATGGCACAAAAGGAAGAACCAACAAAGTCTTCTGAAGAAGGCAAGCCCAAGCCCCCTCCGGCGGACACAGCCATGGCTATTGCTTCAACCGCAGCTCCCGCTCCTTCCTGGTTCACGCCCAAGAGGTTACTTGTTATCTTTTGCTTGATTAACTTGATAAATTATGTGGACCGAGGAACAATAGCAAGCAATGGTGTCAATGGGAGCCTCGGAACTTGTACAGATAGTGATATATGCACGGCTGGTACCGGGATACAGGGGGAGTTTCACTTGAATAACTTTGAAGATGGATGTCTGTCATCAGCTTTTATGGTTGGACTGCTTATTGCTTCTCCTATATTTGCATCATTAGCAAAGAGTGTAAGTCCATTTAGGCTCATTGGAGTTGGATTATCAGTTTGGACCTTTGCGATAGTTGGTTGTGCTTGTTCATTCGATTTCTGGTCCATTACTATTTTCCGCATGCTAGTTGGTGTTGGGGAAGCTTCGTTTATTAGTCTTGCAGCTCCATTCATTGACGATAATGCCCCAGCTGCTCAGAAAACTGCATGGCTTGGTATGTTCTACATGTGCATACCAAGTGGATATGCACTTGGCTATGTTTATGGTGGGGTGGTTGGAAGTCACACCAAGTGGCGTTATGCATTTGTGGGGGAAGCAATCCTTATGCTTCCATTTGCTATTCTAGGGTTTGTTATGAAGCCTTTGCAGCTGAAAGGTTTTTTTCATCCTGAATCAAAAAAAGCACTGACAGCAGTGGAAACGGCTGTTGAAGAAGTTCAAGTTAGTGATACATATGATATTTCGAATGGTGAACAAGCTTCCTGTTCGATGAAGGAACATGTGAGAAATTCTACCTTACAAAAATCTTCCAAGTCAAAATTGGTATCAATCATAAAGAATCAAATTTCAAGGTTTTTGAAAGATGTGAAGGTGCTATTGGTGGATAAGGTTTATGTTGTGAATGTTCTAGGTTACATAGCATACAACTTTGTCATAGGTGCATACTCATATTGGGGGCCTAAGGCTGGATATAATATATATCATATGACTGATGCAGATCTTATATTTGGAGGTATTACAATTGTGTGCGGAATATTGGGCACACTAGCGGGTGGCTTTCTTCTGGATTATATGACTAACACTATCTCTAATGCTTTTAAGCTTCTTTCAGTGGTAACGTTAATTGGGGGTGCCTTTTGCTTTGGTGCCTTTTGCTTCAAGAATATGTATGCTTTCCTAGCTCTTTTCGCAATCGGTGAACTACTTGTCTTTGCAACTCAGGGTCCTGTAAATTACATTAATCTCCATTGTGTTAAACCTAATATGAGGCCATTATCTATGGCTATTTCAACGGTTTCAATTCATATCTTCGGTGATGTACCTTCCGCACCTCTTGTTGGAGTTCTCCAGGATGCAATTAACAACTGGAGGAAGACTGCTCTTATTCTAACAGCTATTTTGTTTCCATCAGCTATAATATGGTTTATAGGAATCTTTCTGCACAGCGTGGATAGATTTAACGAGGAAAGTGAGCATCAGGTGACCATCCAAACCGAAAGGTCTAACACAACTCCGTTGCTTGAAGGGAAGAAGATAGAAACAACTGAATCTGTTGCTGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

543

Amino Acids

58.76

Weight (kDa)

6.32

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 245
AccB1I GGYRCC 3 cut(s) 245, 1193, 1208
AccBSI CCGCTC 1 cut(s) 101
AciI CCGC 7 cut(s) 62, 90, 99, 460, 1092, 1112, 1389
AclI AACGTT 1 cut(s) 1181
AcsI RAATTY 2 cut(s) 850, 906
AcuI CTGAAG 1 cut(s) 54
AdeI CACNNNGTG 2 cut(s) 633, 1510
AfaI GTAC 3 cut(s) 220, 247, 1383
AfiI CCNNNNNNNGG 3 cut(s) 61, 1028, 1400
AflIII ACRYGT 2 cut(s) 567, 841
AjnI CCWGG 2 cut(s) 107, 1410
AjuI GAANNNNNNNTTGG 4 cut(s) 13, 45, 923, 955
AoxI GGCC 2 cut(s) 1019, 1331
ApeKI GCWGC 4 cut(s) 92, 503, 533, 709
ApoI RAATTY 2 cut(s) 850, 906
AseI ATTAAT 1 cut(s) 1302
Asp700I GAANNNNTTC 1 cut(s) 777
Asp718I GGTACC 1 cut(s) 245
AspS9I GGNCC 5 cut(s) 169, 402, 444, 1019, 1286
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 4 cut(s) 824, 1271, 1388, 1555
AsuII TTCGAA 1 cut(s) 806
AvaII GGWCC 4 cut(s) 169, 402, 444, 1286
AxyI CCTNAGG 1 cut(s) 1023
BaeGI GKGCMC 1 cut(s) 1106
BanI GGYRCC 3 cut(s) 245, 1193, 1208
BbsI GAAGAC 2 cut(s) 21, 1442
BbvI GCAGC 4 cut(s) 104, 515, 520, 721
BccI CCATC 3 cut(s) 281, 1477, 1556
BceAI ACGGC 2 cut(s) 255, 780
BciT130I CCWGG 2 cut(s) 109, 1412
BciVI GTATCC 1 cut(s) 246
BcnI CCSGG 1 cut(s) 250
BfaI CTAG 5 cut(s) 467, 686, 971, 1109, 1241
BfuI GTATCC 1 cut(s) 246
BglII AGATCT 1 cut(s) 1060
BisI GCNGC 4 cut(s) 93, 504, 534, 710
BlsI GCNGC 4 cut(s) 94, 505, 535, 711
Bme1390I CCNGG 3 cut(s) 109, 250, 1412
Bme18I GGWCC 4 cut(s) 169, 402, 444, 1286
BmgT120I GGNCC 5 cut(s) 169, 402, 444, 1019, 1286
BmiI GGNNCC 6 cut(s) 206, 247, 1020, 1195, 1210, 1287
BmrFI CCNGG 3 cut(s) 109, 250, 1412
BmsI GCATC 4 cut(s) 353, 1045, 1405, 1546
BpiI GAAGAC 2 cut(s) 21, 1442
BpmI CTGGAG 2 cut(s) 1394, 1450
Bpu14I TTCGAA 1 cut(s) 806
BpuMI CCSGG 1 cut(s) 250
BsaBI GATNNNNATC 1 cut(s) 1059
BsaJI CCNNGG 3 cut(s) 72, 172, 208
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 1028, 1400
Bse1I ACTGG 1 cut(s) 1433
Bse21I CCTNAGG 1 cut(s) 1023
Bse3DI GCAATG 1 cut(s) 196
Bse8I GATNNNNATC 1 cut(s) 1059
BseBI CCWGG 2 cut(s) 109, 1412
BseDI CCNNGG 3 cut(s) 72, 172, 208
BseGI GGATG 4 cut(s) 296, 727, 1420, 1548
BseJI GATNNNNATC 1 cut(s) 1059
BseLI CCNNNNNNNGG 3 cut(s) 61, 1028, 1400
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 2 cut(s) 551, 1295
BseNI ACTGG 1 cut(s) 1433
BseSI GKGCMC 1 cut(s) 1106
BseXI GCAGC 4 cut(s) 104, 515, 520, 721
BseYI CCCAGC 1 cut(s) 529
BsgI GTGCAG 1 cut(s) 1487
BshFI GGCC 2 cut(s) 1021, 1333
BshNI GGYRCC 3 cut(s) 245, 1193, 1208
BsiSI CCGG 2 cut(s) 59, 249
BslI CCNNNNNNNGG 3 cut(s) 61, 1028, 1400
BsnI GGCC 2 cut(s) 1021, 1333
Bsp119I TTCGAA 1 cut(s) 806
Bsp1286I GDGCHC 1 cut(s) 1106
Bsp1407I TGTACA 1 cut(s) 218
Bsp143I GATC 1 cut(s) 1060
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 7 cut(s) 62, 90, 99, 460, 1092, 1112, 1389
BspANI GGCC 2 cut(s) 1021, 1333
BspCNI CTCAG 2 cut(s) 550, 1294
BspLI GGNNCC 6 cut(s) 206, 247, 1020, 1195, 1210, 1287
BspT104I TTCGAA 1 cut(s) 806
BspT107I GGYRCC 3 cut(s) 245, 1193, 1208
BsrBI CCGCTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 196
BsrGI TGTACA 1 cut(s) 218
BsrI ACTGG 1 cut(s) 1433
BssECI CCNNGG 3 cut(s) 72, 172, 208
BssMI GATC 1 cut(s) 1060
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 109, 1412
Bst4CI ACNGT 1 cut(s) 1357
BstAUI TGTACA 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 806
BstC8I GCNNGC 3 cut(s) 44, 187, 464
BstDEI CTNAG 3 cut(s) 537, 1023, 1281
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 1543
BstF5I GGATG 4 cut(s) 296, 727, 1420, 1548
BstKTI GATC 1 cut(s) 1063
BstMBI GATC 1 cut(s) 1060
BstMWI GCNNNNNNNGC 9 cut(s) 68, 89, 98, 350, 533, 709, 752, 1241, 1250
BstNI CCWGG 2 cut(s) 109, 1412
BstNSI RCATGY 3 cut(s) 466, 571, 845
BstPI GGTNACC 1 cut(s) 1543
BstSCI CCNGG 3 cut(s) 107, 248, 1410
BstSLI GKGCMC 1 cut(s) 1106
BstV1I GCAGC 4 cut(s) 104, 515, 520, 721
BstV2I GAAGAC 2 cut(s) 21, 1442
BstX2I RGATCY 1 cut(s) 1060
BstYI RGATCY 1 cut(s) 1060
Bsu36I CCTNAGG 1 cut(s) 1023
BsuI GTATCC 1 cut(s) 246
BsuRI GGCC 2 cut(s) 1021, 1333
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 4 cut(s) 296, 727, 1420, 1548
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 746, 762, 1179
Cac8I GCNNGC 3 cut(s) 44, 187, 464
Cfr13I GGNCC 5 cut(s) 169, 402, 444, 1019, 1286
Csp6I GTAC 3 cut(s) 219, 246, 1382
CviAII CATG 6 cut(s) 73, 463, 549, 568, 842, 1626
CviQI GTAC 3 cut(s) 219, 246, 1382
DdeI CTNAG 3 cut(s) 537, 1023, 1281
DpnI GATC 1 cut(s) 1062
DpnII GATC 1 cut(s) 1060
DraIII CACNNNGTG 2 cut(s) 633, 1510
EciI GGCGGA 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 72
Eco47I GGWCC 4 cut(s) 169, 402, 444, 1286
Eco57I CTGAAG 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 1023
Eco91I GGTNACC 1 cut(s) 1543
EcoO109I RGGNCCY 2 cut(s) 1019, 1286
EcoO65I GGTNACC 1 cut(s) 1543
EcoRII CCWGG 2 cut(s) 107, 1410
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 646
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 6 cut(s) 76, 466, 552, 571, 845, 1629
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FatI CATG 6 cut(s) 72, 462, 548, 567, 841, 1625
FauI CCCGC 2 cut(s) 106, 1105
FauNDI CATATG 2 cut(s) 796, 1047
Fnu4HI GCNGC 4 cut(s) 93, 504, 534, 710
FokI GGATG 4 cut(s) 303, 714, 1427, 1535
Fsp4HI GCNGC 4 cut(s) 93, 504, 534, 710
FspBI CTAG 5 cut(s) 467, 686, 971, 1109, 1241
GluI GCNGC 4 cut(s) 93, 504, 534, 710
GsaI CCCAGC 1 cut(s) 533
GsuI CTGGAG 2 cut(s) 1394, 1450
HaeIII GGCC 2 cut(s) 1021, 1333
HapII CCGG 2 cut(s) 59, 249
Hin1II CATG 6 cut(s) 76, 466, 552, 571, 845, 1629
HindIII AAGCTT 3 cut(s) 483, 819, 1163
HinfI GANTC 4 cut(s) 734, 901, 1494, 1610
HpaII CCGG 2 cut(s) 59, 249
HphI GGTGA 4 cut(s) 824, 1271, 1388, 1555
Hpy166II GTNNAC 4 cut(s) 114, 169, 815, 1262
Hpy188I TCNGA 3 cut(s) 34, 212, 540
Hpy188III TCNNGA 3 cut(s) 731, 1127, 1222
Hpy8I GTNNAC 4 cut(s) 114, 169, 815, 1262
HpyAV CCTTC 6 cut(s) 32, 114, 829, 925, 1395, 1580
HpyCH4III ACNGT 1 cut(s) 1357
HpyCH4IV ACGT 1 cut(s) 1181
HpyF10VI GCNNNNNNNGC 9 cut(s) 68, 89, 98, 350, 533, 709, 752, 1241, 1250
HpyF3I CTNAG 3 cut(s) 537, 1023, 1281
HpySE526I ACGT 1 cut(s) 1181
Hsp92II CATG 6 cut(s) 76, 466, 552, 571, 845, 1629
KpnI GGTACC 1 cut(s) 249
Kzo9I GATC 1 cut(s) 1060
LmnI GCTCC 4 cut(s) 100, 106, 204, 511
Lsp1109I GCAGC 4 cut(s) 104, 515, 520, 721
LweI GCATC 4 cut(s) 353, 1045, 1405, 1546
MaeI CTAG 5 cut(s) 467, 686, 971, 1109, 1241
MaeII ACGT 1 cut(s) 1181
MaeIII GTNAC 5 cut(s) 126, 623, 974, 1177, 1543
MalI GATC 1 cut(s) 1062
MbiI CCGCTC 1 cut(s) 101
MboI GATC 1 cut(s) 1060
MfeI CAATTG 1 cut(s) 1083
MflI RGATCY 1 cut(s) 1060
MhlI GDGCHC 1 cut(s) 1106
MluCI AATT 9 cut(s) 160, 850, 881, 906, 1083, 1185, 1294, 1362, 1419
MmeI TCCRAC 4 cut(s) 295, 367, 598, 1381
MnlI CCTC 9 cut(s) 66, 117, 167, 218, 1067, 1323, 1404, 1425, 1519
Mph1103I ATGCAT 1 cut(s) 646
MroXI GAANNNNTTC 1 cut(s) 777
MseI TTAA 7 cut(s) 150, 1161, 1184, 1302, 1317, 1422, 1521
MslI CAYNNNNRTG 1 cut(s) 71
MspA1I CMGCKG 2 cut(s) 533, 712
MspI CCGG 2 cut(s) 59, 249
MspR9I CCNGG 3 cut(s) 109, 250, 1412
MunI CAATTG 1 cut(s) 1083
MvaI CCWGG 2 cut(s) 109, 1412
MwoI GCNNNNNNNGC 9 cut(s) 68, 89, 98, 350, 533, 709, 752, 1241, 1250
NciI CCSGG 1 cut(s) 250
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 2 cut(s) 796, 1047
NdeII GATC 1 cut(s) 1060
NlaIII CATG 6 cut(s) 76, 466, 552, 571, 845, 1629
NlaIV GGNNCC 6 cut(s) 206, 247, 1020, 1195, 1210, 1287
NmuCI GTSAC 2 cut(s) 623, 1543
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 3 cut(s) 466, 571, 845
NspV TTCGAA 1 cut(s) 806
PaeI GCATGC 1 cut(s) 466
PciI ACATGT 2 cut(s) 567, 841
PdmI GAANNNNTTC 1 cut(s) 777
PfeI GAWTC 4 cut(s) 734, 901, 1494, 1610
PfoI TCCNGGA 1 cut(s) 1410
PkrI GCNGC 4 cut(s) 94, 505, 535, 711
PpuMI RGGWCCY 1 cut(s) 1286
PscI ACATGT 2 cut(s) 567, 841
PshBI ATTAAT 1 cut(s) 1302
Psp1406I AACGTT 1 cut(s) 1181
Psp5II RGGWCCY 1 cut(s) 1286
Psp6I CCWGG 2 cut(s) 107, 1410
PspEI GGTNACC 1 cut(s) 1543
PspFI CCCAGC 1 cut(s) 529
PspGI CCWGG 2 cut(s) 107, 1410
PspN4I GGNNCC 6 cut(s) 206, 247, 1020, 1195, 1210, 1287
PspPI GGNCC 5 cut(s) 169, 402, 444, 1019, 1286
PspPPI RGGWCCY 1 cut(s) 1286
PsuI RGATCY 1 cut(s) 1060
PvuII CAGCTG 2 cut(s) 533, 712
RsaI GTAC 3 cut(s) 220, 247, 1383
RsaNI GTAC 3 cut(s) 219, 246, 1382
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 7 cut(s) 150, 1161, 1184, 1302, 1317, 1422, 1521
SatI GCNGC 4 cut(s) 93, 504, 534, 710
Sau3AI GATC 1 cut(s) 1060
Sau96I GGNCC 5 cut(s) 169, 402, 444, 1019, 1286
ScrFI CCNGG 3 cut(s) 109, 250, 1412
SduI GDGCHC 1 cut(s) 1106
SfaNI GCATC 4 cut(s) 353, 1045, 1405, 1546
SfuI TTCGAA 1 cut(s) 806
SinI GGWCC 4 cut(s) 169, 402, 444, 1286
SmiMI CAYNNNNRTG 1 cut(s) 71
SphI GCATGC 1 cut(s) 466
Sse9I AATT 9 cut(s) 160, 850, 881, 906, 1083, 1185, 1294, 1362, 1419
SsiI CCGC 7 cut(s) 62, 90, 99, 460, 1092, 1112, 1389
SspI AATATT 1 cut(s) 1098
SspMI CTAG 5 cut(s) 467, 686, 971, 1109, 1241
StyD4I CCNGG 3 cut(s) 107, 248, 1410
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 1357
TaiI ACGT 1 cut(s) 1184
TaqI TCGA 3 cut(s) 435, 806, 830
TaqII GACCGA 1 cut(s) 186
TasI AATT 9 cut(s) 160, 850, 881, 906, 1083, 1185, 1294, 1362, 1419
TatI WGTACW 1 cut(s) 218
TfiI GAWTC 4 cut(s) 734, 901, 1494, 1610
Tru1I TTAA 7 cut(s) 150, 1161, 1184, 1302, 1317, 1422, 1521
Tru9I TTAA 7 cut(s) 150, 1161, 1184, 1302, 1317, 1422, 1521
TscAI CASTG 3 cut(s) 753, 762, 1179
TseFI GTSAC 2 cut(s) 623, 1543
TseI GCWGC 4 cut(s) 92, 503, 533, 709
Tsp45I GTSAC 2 cut(s) 623, 1543
TspDTI ATGAA 6 cut(s) 420, 502, 713, 716, 848, 1355
TspGWI ACGGA 1 cut(s) 1566
TspRI CASTG 3 cut(s) 753, 762, 1179
VpaK11BI GGWCC 4 cut(s) 169, 402, 444, 1286
VspI ATTAAT 1 cut(s) 1302
XapI RAATTY 2 cut(s) 850, 906
XceI RCATGY 3 cut(s) 466, 571, 845
XcmI CCANNNNNNNNNTGG 1 cut(s) 880
XmnI GAANNNNTTC 1 cut(s) 777
XspI CTAG 5 cut(s) 467, 686, 971, 1109, 1241
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.