Rroxscaffold_7G00199020

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001054
Physical Location & Seq
Forward (+)
44868337 .. 44877414
9078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00199020.1

Sequence Viewer

Length: 1587 bp
ATGAAACAACCCGCAGAGAATCGAAACCCCGCGCACTCCGGAAAAGCTGAATCTAAAATCGAATCATTCCAACAGGTTACTTCTCATATTCTGTGTGATCAACTTGGTAAATTATGTGGACCGAGGAGCAATATCAAGCAACGGCGTGAATGGGAACCTTCCAATTTGCAGGAATGGGGGCATTTTTTACTGAACAATTTTTCAGTTGGATGTCTATCGTCAGCTTTTATGGTTGGACTTCTGGTGGCTTCTCCAATTTTTGCAGCACTAGCAAAGAGTGTAAATCCATTTAAGCTCATTGGAGTTGGATTGTCAATTTGGACTTTTGCTGTAACTGGTTGTGGTTTTTCATTCAACTTCTTGTCTATCTTAATCTGCCGCATGCTAGTTGGTGTTGGTGAGGCTTCGTTTATTAGTCTTGCAGCTCCGTTCATTGATGATAATGCCCCAGCTGCTCAGGTATTGGATCTTTTGTTTCTGTTTATACCTATTCTTTTGCATTTGTTTGACCTGAGAAAACTCTATGGCTTTCAACATTCTACATGTGCATACCATCTGGATATGCACTTGGTTATGTTTACGGAGGCTTGGTGGGAACTACTCTCAGTTGGCGTGCTGCATTTTGGGGGAAGGAATTCTGATGCTTCCATTTGCTATTCTAGGATTTGTTTTAAGCATGATGAATCAATTGAAGCATTGACTGTGCAGGAGACGGCTGTCTCAGAAGTTCAAGGTGCTTTGGACCCCAAGGATAGGGCAGTTTCAATGAAGGAAGATGTAACAAATTCTACCATACACAAACCTTCCAAGCCAAAAATTGCAACAAAAATAAAGAATCATCTTTCAAGGTTTATGAAAGATATGAAGGATCTTTTGGTCGATAAGGTTTATGTTGTTAATGTTCTTGGTTATGTAGCATACAACTTTGTAATTGGTTCGTACTCATATTGGGGGCCTAAGGCTGGTTATAATATCTATCATATGACTAATGCAGATATGATTTTTGGAGGTATTACAATTGTGTGTGGAATATTCGGAACACTAGCAGGAGGCTTTCTTCTGGATTTCATGTCTAACACTATGTCCAATGCTTTCAAGCTTCTTTCAACAGCAACGCTTTTTGGGGCAATATTATGCTTTGGTGCGTTTTGCTTGAAGAACATGTATGGTTTCCTAGCTCTTTTTGCAGTAGGGCAACTACTTCTCTTTGCAACTCAGGGTCCTGTAAATTTTGTTTCTCTCCATTGTGTTAAACCTAGTTTGAGGCCACTATCTATGGCCATTTGTACTGTTGCAATTCACATATTTGGAGATGTGCCTTCCTCACCTCTTGTTGGAGTCATCCAGGATCACATCCAGAATTGGAGGTCAACTGCTCTTATTCTAACATCCGTTTTGATTCCTGCAGCGGCCATATGGTTTACAGCAATTTTCATGGACAGCGTGGATAGATATGACGAAGAAAGTGATGATGAAGAAAGTGATGATGAGATAGAAACAACTGAAATGCCGTCACTTGAAGAGAAGAAGACACAGAAAACAACTGAATCTGCGGCTCAACCTGTCACATATTCAACTGTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

528

Amino Acids

58.27

Weight (kDa)

5.84

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 64 - 152 1.2e-07 Major Facilitator Superfamily
OATP PF03137 262 - 384 2.9e-12 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 383 - 451 1.7e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 969
AccII CGCG 1 cut(s) 32
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 5 cut(s) 12, 30, 379, 1409, 1553
AclWI GGATC 3 cut(s) 474, 876, 1356
AcoI YGGCCR 2 cut(s) 1278, 1410
AcsI RAATTY 3 cut(s) 634, 784, 1228
AfaI GTAC 2 cut(s) 941, 1288
AfiI CCNNNNNNNGG 3 cut(s) 753, 962, 1334
AflIII ACRYGT 2 cut(s) 542, 1161
AjnI CCWGG 1 cut(s) 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AluBI AGCT 7 cut(s) 47, 224, 295, 425, 452, 1099, 1178
AluI AGCT 7 cut(s) 47, 224, 295, 425, 452, 1099, 1178
Alw26I GTCTC 2 cut(s) 704, 724
AlwI GGATC 3 cut(s) 474, 876, 1356
Aor13HI TCCGGA 1 cut(s) 38
AoxI GGCC 4 cut(s) 953, 1265, 1278, 1410
ApeKI GCWGC 5 cut(s) 263, 422, 452, 616, 1406
ApoI RAATTY 3 cut(s) 634, 784, 1228
Asp700I GAANNNNTTC 1 cut(s) 634
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 4 cut(s) 119, 742, 953, 1220
AsuHPI GGTGA 2 cut(s) 410, 1317
AvaII GGWCC 3 cut(s) 119, 742, 1220
AxyI CCTNAGG 1 cut(s) 957
BalI TGGCCA 1 cut(s) 1280
BbsI GAAGAC 1 cut(s) 1535
BbvI GCAGC 5 cut(s) 275, 434, 439, 603, 1418
BccI CCATC 1 cut(s) 561
BceAI ACGGC 3 cut(s) 158, 729, 1495
BciT130I CCWGG 1 cut(s) 1346
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 704, 724
BfaI CTAG 7 cut(s) 269, 386, 660, 1043, 1175, 1257, 1585
BfmI CTRYAG 1 cut(s) 1404
BisI GCNGC 8 cut(s) 264, 379, 423, 453, 617, 1407, 1410, 1554
BlsI GCNGC 8 cut(s) 265, 380, 424, 454, 618, 1408, 1411, 1555
Bme1390I CCNGG 1 cut(s) 1346
Bme18I GGWCC 3 cut(s) 119, 742, 1220
BmgT120I GGNCC 4 cut(s) 119, 742, 953, 1220
BmiI GGNNCC 4 cut(s) 156, 744, 954, 1221
BmrFI CCNGG 1 cut(s) 1346
BmsI GCATC 1 cut(s) 631
BoxI GACNNNNGTC 1 cut(s) 716
BpiI GAAGAC 1 cut(s) 1535
Bpu10I CCTNAGC 1 cut(s) 456
BsaBI GATNNNNATC 1 cut(s) 214
BsaJI CCNNGG 2 cut(s) 122, 747
BsaWI WCCGGW 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bsc4I CCNNNNNNNGG 3 cut(s) 753, 962, 1334
Bse1I ACTGG 1 cut(s) 340
Bse21I CCTNAGG 1 cut(s) 957
Bse8I GATNNNNATC 1 cut(s) 214
BseAI TCCGGA 1 cut(s) 38
BseBI CCWGG 1 cut(s) 1346
BseDI CCNNGG 2 cut(s) 122, 747
BseGI GGATG 4 cut(s) 215, 1341, 1353, 1388
BseJI GATNNNNATC 1 cut(s) 214
BseLI CCNNNNNNNGG 3 cut(s) 753, 962, 1334
BseMII CTCAG 5 cut(s) 470, 503, 618, 735, 1229
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 5 cut(s) 275, 434, 439, 603, 1418
BseYI CCCAGC 1 cut(s) 448
BsgI GTGCAG 1 cut(s) 725
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 4 cut(s) 955, 1267, 1280, 1412
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 3 cut(s) 753, 962, 1334
BsmAI GTCTC 2 cut(s) 704, 724
BsmBI CGTCTC 1 cut(s) 704
BsnI GGCC 4 cut(s) 955, 1267, 1280, 1412
Bsp13I TCCGGA 1 cut(s) 38
Bsp143I GATC 4 cut(s) 97, 466, 868, 1348
BspACI CCGC 5 cut(s) 12, 30, 379, 1409, 1553
BspANI GGCC 4 cut(s) 955, 1267, 1280, 1412
BspCNI CTCAG 5 cut(s) 469, 504, 617, 734, 1228
BspEI TCCGGA 1 cut(s) 38
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 4 cut(s) 156, 744, 954, 1221
BspMAI CTGCAG 1 cut(s) 1408
BspPI GGATC 3 cut(s) 474, 876, 1356
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 2 cut(s) 122, 747
BssMI GATC 4 cut(s) 97, 466, 868, 1348
BssT1I CCWWGG 1 cut(s) 747
Bst2UI CCWGG 1 cut(s) 1346
Bst4CI ACNGT 3 cut(s) 703, 1291, 1579
Bst6I CTCTTC 1 cut(s) 1515
BstC8I GCNNGC 2 cut(s) 383, 614
BstDEI CTNAG 6 cut(s) 456, 512, 604, 721, 957, 1215
BstF5I GGATG 4 cut(s) 215, 1341, 1353, 1388
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 4 cut(s) 100, 469, 871, 1351
BstMAI GTCTC 2 cut(s) 704, 724
BstMBI GATC 4 cut(s) 97, 466, 868, 1348
BstMWI GCNNNNNNNGC 3 cut(s) 269, 452, 1184
BstNI CCWGG 1 cut(s) 1346
BstNSI RCATGY 3 cut(s) 385, 546, 1165
BstPAI GACNNNNGTC 1 cut(s) 716
BstSCI CCNGG 1 cut(s) 1344
BstSFI CTRYAG 1 cut(s) 1404
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 5 cut(s) 275, 434, 439, 603, 1418
BstV2I GAAGAC 1 cut(s) 1535
BstX2I RGATCY 2 cut(s) 466, 868
BstYI RGATCY 2 cut(s) 466, 868
Bsu36I CCTNAGG 1 cut(s) 957
BsuRI GGCC 4 cut(s) 955, 1267, 1280, 1412
BtsCI GGATG 4 cut(s) 215, 1341, 1353, 1388
Cac8I GCNNGC 2 cut(s) 383, 614
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 4 cut(s) 119, 742, 953, 1220
Csp6I GTAC 2 cut(s) 940, 1287
CviAII CATG 6 cut(s) 382, 543, 677, 1069, 1162, 1435
CviQI GTAC 2 cut(s) 940, 1287
DdeI CTNAG 6 cut(s) 456, 512, 604, 721, 957, 1215
DpnI GATC 4 cut(s) 99, 468, 870, 1350
DpnII GATC 4 cut(s) 97, 466, 868, 1348
EaeI YGGCCR 2 cut(s) 1278, 1410
Eam1104I CTCTTC 1 cut(s) 1515
EarI CTCTTC 1 cut(s) 1515
Eco130I CCWWGG 1 cut(s) 747
Eco47I GGWCC 3 cut(s) 119, 742, 1220
Eco81I CCTNAGG 1 cut(s) 957
EcoO109I RGGNCCY 2 cut(s) 953, 1220
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 1344
EcoT14I CCWWGG 1 cut(s) 747
ErhI CCWWGG 1 cut(s) 747
Esp3I CGTCTC 1 cut(s) 704
FaeI CATG 6 cut(s) 385, 546, 680, 1072, 1165, 1438
FatI CATG 6 cut(s) 381, 542, 676, 1068, 1161, 1434
FauI CCCGC 2 cut(s) 19, 37
FauNDI CATATG 2 cut(s) 981, 1415
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 8 cut(s) 264, 379, 423, 453, 617, 1407, 1410, 1554
FokI GGATG 4 cut(s) 222, 1328, 1340, 1375
Fsp4HI GCNGC 8 cut(s) 264, 379, 423, 453, 617, 1407, 1410, 1554
FspBI CTAG 7 cut(s) 269, 386, 660, 1043, 1175, 1257, 1585
GlaI GCGC 1 cut(s) 33
GluI GCNGC 8 cut(s) 264, 379, 423, 453, 617, 1407, 1410, 1554
GsaI CCCAGC 1 cut(s) 452
HaeIII GGCC 4 cut(s) 955, 1267, 1280, 1412
HapII CCGG 1 cut(s) 39
HhaI GCGC 1 cut(s) 34
Hin1II CATG 6 cut(s) 385, 546, 680, 1072, 1165, 1438
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 1371
HindII GTYRAC 1 cut(s) 1371
HindIII AAGCTT 1 cut(s) 1097
HinfI GANTC 8 cut(s) 19, 50, 62, 683, 835, 1338, 1399, 1547
HpaII CCGG 1 cut(s) 39
HphI GGTGA 2 cut(s) 410, 1317
Hpy166II GTNNAC 4 cut(s) 119, 579, 1371, 1422
Hpy188I TCNGA 3 cut(s) 640, 724, 1037
Hpy188III TCNNGA 4 cut(s) 39, 557, 1061, 1357
Hpy8I GTNNAC 4 cut(s) 119, 579, 1371, 1422
HpyAV CCTTC 6 cut(s) 168, 624, 763, 813, 859, 1329
HpyCH4III ACNGT 3 cut(s) 703, 1291, 1579
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 452, 1184
HpyF3I CTNAG 6 cut(s) 456, 512, 604, 721, 957, 1215
Hsp92II CATG 6 cut(s) 385, 546, 680, 1072, 1165, 1438
HspAI GCGC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 4 cut(s) 97, 466, 868, 1348
LmnI GCTCC 2 cut(s) 126, 430
Lsp1109I GCAGC 5 cut(s) 275, 434, 439, 603, 1418
LweI GCATC 1 cut(s) 631
MaeI CTAG 7 cut(s) 269, 386, 660, 1043, 1175, 1257, 1585
MaeIII GTNAC 5 cut(s) 76, 331, 778, 1512, 1564
MalI GATC 4 cut(s) 99, 468, 870, 1350
MboI GATC 4 cut(s) 97, 466, 868, 1348
MboII GAAGA 8 cut(s) 785, 1049, 1168, 1472, 1487, 1532, 1537, 1540
MfeI CAATTG 2 cut(s) 687, 1017
MflI RGATCY 2 cut(s) 466, 868
MlsI TGGCCA 1 cut(s) 1280
MluNI TGGCCA 1 cut(s) 1280
MlyI GAGTC 1 cut(s) 1347
MmeI TCCRAC 5 cut(s) 94, 187, 214, 286, 1315
MnlI CCTC 9 cut(s) 117, 394, 577, 1001, 1043, 1257, 1333, 1338, 1359
Mox20I TGGCCA 1 cut(s) 1280
MroI TCCGGA 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 634
MscI TGGCCA 1 cut(s) 1280
MseI TTAA 5 cut(s) 291, 371, 672, 897, 1251
Msp20I TGGCCA 1 cut(s) 1280
MspA1I CMGCKG 2 cut(s) 452, 1409
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 1 cut(s) 1346
MunI CAATTG 2 cut(s) 687, 1017
MvaI CCWGG 1 cut(s) 1346
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 3 cut(s) 269, 452, 1184
NdeI CATATG 2 cut(s) 981, 1415
NdeII GATC 4 cut(s) 97, 466, 868, 1348
NlaIII CATG 6 cut(s) 385, 546, 680, 1072, 1165, 1438
NlaIV GGNNCC 4 cut(s) 156, 744, 954, 1221
NmuCI GTSAC 2 cut(s) 1512, 1564
NspI RCATGY 3 cut(s) 385, 546, 1165
PaeI GCATGC 1 cut(s) 385
PciI ACATGT 2 cut(s) 542, 1161
PdmI GAANNNNTTC 1 cut(s) 634
PfeI GAWTC 7 cut(s) 19, 50, 62, 683, 835, 1399, 1547
PfoI TCCNGGA 1 cut(s) 1344
PkrI GCNGC 8 cut(s) 265, 380, 424, 454, 618, 1408, 1411, 1555
PleI GAGTC 1 cut(s) 1346
PpsI GAGTC 1 cut(s) 1346
PpuMI RGGWCCY 1 cut(s) 1220
PscI ACATGT 2 cut(s) 542, 1161
PshAI GACNNNNGTC 1 cut(s) 716
PsiI TTATAA 1 cut(s) 969
Psp5II RGGWCCY 1 cut(s) 1220
Psp6I CCWGG 1 cut(s) 1344
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 1 cut(s) 1344
PspN4I GGNNCC 4 cut(s) 156, 744, 954, 1221
PspPI GGNCC 4 cut(s) 119, 742, 953, 1220
PspPPI RGGWCCY 1 cut(s) 1220
PstI CTGCAG 1 cut(s) 1408
PsuI RGATCY 2 cut(s) 466, 868
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 2 cut(s) 941, 1288
RsaNI GTAC 2 cut(s) 940, 1287
SaqAI TTAA 5 cut(s) 291, 371, 672, 897, 1251
SatI GCNGC 8 cut(s) 264, 379, 423, 453, 617, 1407, 1410, 1554
Sau3AI GATC 4 cut(s) 97, 466, 868, 1348
Sau96I GGNCC 4 cut(s) 119, 742, 953, 1220
SchI GAGTC 1 cut(s) 1347
ScrFI CCNGG 1 cut(s) 1346
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 1 cut(s) 1404
SinI GGWCC 3 cut(s) 119, 742, 1220
SphI GCATGC 1 cut(s) 385
SsiI CCGC 5 cut(s) 12, 30, 379, 1409, 1553
SspI AATATT 2 cut(s) 1032, 1131
SspMI CTAG 7 cut(s) 269, 386, 660, 1043, 1175, 1257, 1585
StyD4I CCNGG 1 cut(s) 1344
StyI CCWWGG 1 cut(s) 747
TaaI ACNGT 3 cut(s) 703, 1291, 1579
TaqI TCGA 3 cut(s) 22, 60, 879
TaqII GACCGA 1 cut(s) 136
TatI WGTACW 1 cut(s) 1286
TauI GCSGC 3 cut(s) 381, 1412, 1556
TfiI GAWTC 7 cut(s) 19, 50, 62, 683, 835, 1399, 1547
Tru1I TTAA 5 cut(s) 291, 371, 672, 897, 1251
Tru9I TTAA 5 cut(s) 291, 371, 672, 897, 1251
TseFI GTSAC 2 cut(s) 1512, 1564
TseI GCWGC 5 cut(s) 263, 422, 452, 616, 1406
Tsp45I GTSAC 2 cut(s) 1512, 1564
TspGWI ACGGA 3 cut(s) 417, 596, 1381
VpaK11BI GGWCC 3 cut(s) 119, 742, 1220
XapI RAATTY 3 cut(s) 634, 784, 1228
XceI RCATGY 3 cut(s) 385, 546, 1165
XmnI GAANNNNTTC 1 cut(s) 634
XspI CTAG 7 cut(s) 269, 386, 660, 1043, 1175, 1257, 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.