Rw6G014310

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001054
Physical Location & Seq
Reverse (-)
26442818 .. 26452937
10120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014310.1

Sequence Viewer

Length: 1512 bp
ATGATCGTTTCTTCAATCGCACCTCTTCAGCCTTCATGGTTTTCGCCCAAGAAGTTACTTGTTGTATTTTGTGTGATTAACTTGATAAATTATGTGGACCGAGGAGCAATTGCAAGCAATGGTGTGAATGGGAGTCTGGGAACTTGCAGAGGGGGGGATTTTGACTTGAGCAATTTTGAAGATGGATGCCTATCATCTGCTTTTATGGTTGGACTTCTGGTGGCTTCTCCGATTTTTGCTTCATTAGCAAAGAGTGTGAATCCATTTAGGCTCATTGGAGTTGGATTGTCAGTTTGGACTTTTGCTGTCGTTGGTTGTGGTCTTTCATTCAACTTCTGGTCCATTACCATCTGCCGCATGCTAGTTGGTGTTGGCGAGGCTTCATTTATTAGTCTTGCAGCTCCATTCATTGATGATAATGCCCCAGCTGCTCAGAAAACTGTGTGGCTTGCAATATTCTACATGTGCATACCATCTGGATATGCACTTGGTTATGTATATGGTGGGTTGGTGGGAAGTTATACCAATTGGCGTTTTGCATTCTGGGGGGAGGCAATCCTGATGCTTCCGTTTGCTATTCTAGGGTTTGTCATGAAGCCTTTGCAGCTTAAAGGTTTTATGCCTGCTGAATCAAAACAAGTGCTGACAGGGGTGGAGACGGGTGTCTCAGAAGTTCAAGATTCAGGCGTTTCGAATGGTAAAGATGGTTTGTTTCCAGTGGAGGGAGATGTCAAATATTCTACCATACATAAACAGAATGAGGTGAACTTTGCAACAAAAATAAAGGGTCAAATTTCAAGGTTTACAAAAGATATGAAGGTGCTTTTGATTGAGAAGGTTTATGTTGTTAATGTTCTAGGTTACATAGCGTACAACTTCGTAATTGGTGCGTACTCATATTGGGGGCCTAAGGCTGGTTATAATATATATCATATGACTGATGCAGATTTGATATTTGGAGGTATTACAATTGTGTGTGGAATATTGGGTACCGTAGCAGGAGGCTTTCTTTTGGATTTCATGACCAGCAGTATCCCTAATGCTTTCAAGCTTCTTTCAGCAGCTACGTTTTTTGGGGCAATATTTTGCTTCACTGCCTTTTGCTTCTCAAACATGTATGCTTTCCTAGCTCTATTTGCAGTTGGCGAACTACTTGTCTTTGCAACTCAGGGTCCTGTAAATTACATTTGTCTGCACTGTGTTAAACCTAGTATGAGACCACTAGCTATGGCCATGTCTACTGTTTCTATTCATCTTTTTGGGGATGTGCCTTCCTCACCTCTTGTCGGAGTTCTCGAGGATTGCATCAACAATTGGAGGGTGACTGCTCTTATTCTAACATCTATATTCATTCCAGCGGCTGGTATATGGTTTATAGGAATTTTTCTGCCTAGTGTGGATAGATTTAATGAAGAAAGTGAGAATCAGATATCCACAACTGAAAGGTCCAGCATAACTCCTTTGCTTGAAGGGACGAAGACAGAAACGACTGGATCCGCTGCTGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

503

Amino Acids

54.29

Weight (kDa)

5.5

Isoelectric Point (pI)

28.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 41 - 416 2.6e-29 Major Facilitator Superfamily
OATP PF03137 114 - 367 6.2e-17 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 367 - 434 1.2e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 921
Acc65I GGTACC 1 cut(s) 989
AccB1I GGYRCC 1 cut(s) 989
AccB7I CCANNNNNTGG 1 cut(s) 1361
AccI GTMKAC 1 cut(s) 1238
AciI CCGC 3 cut(s) 355, 1358, 1497
AclWI GGATC 2 cut(s) 1488, 1501
AcoI YGGCCR 1 cut(s) 1230
AcsI RAATTY 2 cut(s) 792, 1380
AcuI CTGAAG 1 cut(s) 11
AfaI GTAC 3 cut(s) 872, 893, 991
AfiI CCNNNNNNNGG 4 cut(s) 722, 914, 1286, 1361
AflIII ACRYGT 2 cut(s) 462, 1113
AgsI TTSAA 7 cut(s) 15, 179, 331, 677, 798, 1048, 1469
AluBI AGCT 7 cut(s) 401, 428, 607, 1051, 1064, 1130, 1226
AluI AGCT 7 cut(s) 401, 428, 607, 1051, 1064, 1130, 1226
Alw26I GTCTC 3 cut(s) 650, 670, 1210
AlwI GGATC 2 cut(s) 1488, 1501
AlwNI CAGNNNCTG 1 cut(s) 1361
Ama87I CYCGRG 1 cut(s) 1295
AoxI GGCC 2 cut(s) 905, 1230
ApeKI GCWGC 5 cut(s) 398, 428, 604, 1061, 1499
ApoI RAATTY 2 cut(s) 792, 1380
ArsI GACNNNNNNTTYG 2 cut(s) 1140, 1172
Asp718I GGTACC 1 cut(s) 989
AspS9I GGNCC 5 cut(s) 97, 339, 905, 1172, 1446
AsuHPI GGTGA 3 cut(s) 775, 1269, 1333
AsuII TTCGAA 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 1295
AvaII GGWCC 4 cut(s) 97, 339, 1172, 1446
AxyI CCTNAGG 1 cut(s) 909
BalI TGGCCA 1 cut(s) 1232
BamHI GGATCC 1 cut(s) 1493
BanI GGYRCC 1 cut(s) 989
BbsI GAAGAC 1 cut(s) 1484
BbvI GCAGC 5 cut(s) 410, 415, 616, 1073, 1486
BccI CCATC 4 cut(s) 176, 356, 481, 698
BciVI GTATCC 1 cut(s) 1043
BcoDI GTCTC 3 cut(s) 650, 670, 1210
BfaI CTAG 7 cut(s) 362, 581, 857, 1127, 1209, 1223, 1392
BfuI GTATCC 1 cut(s) 1043
BisI GCNGC 7 cut(s) 355, 399, 429, 605, 1062, 1359, 1500
BlsI GCNGC 7 cut(s) 356, 400, 430, 606, 1063, 1360, 1501
Bme18I GGWCC 4 cut(s) 97, 339, 1172, 1446
BmeT110I CYCGRG 1 cut(s) 1295
BmgT120I GGNCC 5 cut(s) 97, 339, 905, 1172, 1446
BmiI GGNNCC 4 cut(s) 906, 991, 1173, 1495
BmsI GCATC 4 cut(s) 176, 552, 931, 1314
BoxI GACNNNNGTC 1 cut(s) 662
BpiI GAAGAC 1 cut(s) 1484
Bpu14I TTCGAA 1 cut(s) 692
BpuEI CTTGAG 1 cut(s) 187
BsaBI GATNNNNATC 1 cut(s) 190
BsaI GGTCTC 1 cut(s) 1210
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 4 cut(s) 722, 914, 1286, 1361
Bse1I ACTGG 2 cut(s) 716, 1495
Bse21I CCTNAGG 1 cut(s) 909
Bse3DI GCAATG 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 2 cut(s) 191, 1270
BseJI GATNNNNATC 1 cut(s) 190
BseLI CCNNNNNNNGG 4 cut(s) 722, 914, 1286, 1361
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 3 cut(s) 446, 681, 1181
BseNI ACTGG 2 cut(s) 716, 1495
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 5 cut(s) 410, 415, 616, 1073, 1486
BseYI CCCAGC 1 cut(s) 424
BsgI GTGCAG 1 cut(s) 1178
BshFI GGCC 2 cut(s) 907, 1232
BshNI GGYRCC 1 cut(s) 989
BsiHKCI CYCGRG 1 cut(s) 1295
BslFI GGGAC 1 cut(s) 1486
BslI CCNNNNNNNGG 4 cut(s) 722, 914, 1286, 1361
BsmAI GTCTC 3 cut(s) 650, 670, 1210
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 1486
BsmI GAATGC 1 cut(s) 539
BsnI GGCC 2 cut(s) 907, 1232
Bso31I GGTCTC 1 cut(s) 1210
BsoBI CYCGRG 1 cut(s) 1295
Bsp119I TTCGAA 1 cut(s) 692
Bsp143I GATC 2 cut(s) 3, 1493
BspACI CCGC 3 cut(s) 355, 1358, 1497
BspANI GGCC 2 cut(s) 907, 1232
BspCNI CTCAG 3 cut(s) 445, 680, 1180
BspHI TCATGA 2 cut(s) 591, 1020
BspLI GGNNCC 4 cut(s) 906, 991, 1173, 1495
BspPI GGATC 2 cut(s) 1488, 1501
BspT104I TTCGAA 1 cut(s) 692
BspT107I GGYRCC 1 cut(s) 989
BspTNI GGTCTC 1 cut(s) 1210
BsrDI GCAATG 1 cut(s) 124
BsrI ACTGG 2 cut(s) 716, 1495
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 3, 1493
Bst4CI ACNGT 4 cut(s) 442, 994, 1199, 1243
Bst6I CTCTTC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 692
BstC8I GCNNGC 4 cut(s) 115, 359, 450, 624
BstDEI CTNAG 4 cut(s) 432, 667, 909, 1167
BstF5I GGATG 2 cut(s) 191, 1270
BstKTI GATC 2 cut(s) 6, 1496
BstMAI GTCTC 3 cut(s) 650, 670, 1210
BstMBI GATC 2 cut(s) 3, 1493
BstMWI GCNNNNNNNGC 5 cut(s) 245, 428, 604, 1127, 1136
BstNSI RCATGY 3 cut(s) 361, 466, 1117
BstPAI GACNNNNGTC 1 cut(s) 662
BstV1I GCAGC 5 cut(s) 410, 415, 616, 1073, 1486
BstV2I GAAGAC 1 cut(s) 1484
BstX2I RGATCY 1 cut(s) 1493
BstYI RGATCY 1 cut(s) 1493
Bsu36I CCTNAGG 1 cut(s) 909
BsuI GTATCC 1 cut(s) 1043
BsuRI GGCC 2 cut(s) 907, 1232
BtsCI GGATG 2 cut(s) 191, 1270
BtsI GCAGTG 1 cut(s) 1092
BtsIMutI CAGTG 3 cut(s) 723, 1092, 1195
Cac8I GCNNGC 4 cut(s) 115, 359, 450, 624
CaiI CAGNNNCTG 1 cut(s) 1361
CciI TCATGA 2 cut(s) 591, 1020
Cfr13I GGNCC 5 cut(s) 97, 339, 905, 1172, 1446
Csp6I GTAC 3 cut(s) 871, 892, 990
CviAII CATG 7 cut(s) 36, 358, 463, 592, 1021, 1114, 1234
CviQI GTAC 3 cut(s) 871, 892, 990
DdeI CTNAG 4 cut(s) 432, 667, 909, 1167
DpnI GATC 2 cut(s) 5, 1495
DpnII GATC 2 cut(s) 3, 1493
EaeI YGGCCR 1 cut(s) 1230
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 1210
Eco32I GATATC 1 cut(s) 1431
Eco47I GGWCC 4 cut(s) 97, 339, 1172, 1446
Eco57I CTGAAG 1 cut(s) 11
Eco81I CCTNAGG 1 cut(s) 909
Eco88I CYCGRG 1 cut(s) 1295
EcoO109I RGGNCCY 2 cut(s) 905, 1172
EcoRV GATATC 1 cut(s) 1431
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 7 cut(s) 39, 361, 466, 595, 1024, 1117, 1237
FaqI GGGAC 1 cut(s) 1486
FatI CATG 7 cut(s) 35, 357, 462, 591, 1020, 1113, 1233
FauNDI CATATG 1 cut(s) 933
FblI GTMKAC 1 cut(s) 1238
Fnu4HI GCNGC 7 cut(s) 355, 399, 429, 605, 1062, 1359, 1500
FokI GGATG 2 cut(s) 198, 1277
Fsp4HI GCNGC 7 cut(s) 355, 399, 429, 605, 1062, 1359, 1500
FspBI CTAG 7 cut(s) 362, 581, 857, 1127, 1209, 1223, 1392
GluI GCNGC 7 cut(s) 355, 399, 429, 605, 1062, 1359, 1500
GsaI CCCAGC 1 cut(s) 428
HaeIII GGCC 2 cut(s) 907, 1232
Hin1II CATG 7 cut(s) 39, 361, 466, 595, 1024, 1117, 1237
HindIII AAGCTT 1 cut(s) 1049
HinfI GANTC 5 cut(s) 133, 259, 629, 680, 1423
HphI GGTGA 3 cut(s) 775, 1269, 1333
Hpy166II GTNNAC 4 cut(s) 97, 766, 804, 1239
Hpy188I TCNGA 5 cut(s) 231, 435, 670, 1289, 1428
Hpy188III TCNNGA 6 cut(s) 477, 559, 592, 677, 1021, 1295
Hpy8I GTNNAC 4 cut(s) 97, 766, 804, 1239
HpyAV CCTTC 5 cut(s) 42, 811, 829, 1281, 1463
HpyCH4III ACNGT 4 cut(s) 442, 994, 1199, 1243
HpyCH4IV ACGT 1 cut(s) 1067
HpyF10VI GCNNNNNNNGC 5 cut(s) 245, 428, 604, 1127, 1136
HpyF3I CTNAG 4 cut(s) 432, 667, 909, 1167
HpySE526I ACGT 1 cut(s) 1067
Hsp92II CATG 7 cut(s) 39, 361, 466, 595, 1024, 1117, 1237
KpnI GGTACC 1 cut(s) 993
Kzo9I GATC 2 cut(s) 3, 1493
LmnI GCTCC 2 cut(s) 104, 406
Lsp1109I GCAGC 5 cut(s) 410, 415, 616, 1073, 1486
LweI GCATC 4 cut(s) 176, 552, 931, 1314
MaeI CTAG 7 cut(s) 362, 581, 857, 1127, 1209, 1223, 1392
MaeII ACGT 1 cut(s) 1067
MaeIII GTNAC 3 cut(s) 54, 860, 1321
MalI GATC 2 cut(s) 5, 1495
MboI GATC 2 cut(s) 3, 1493
MboII GAAGA 5 cut(s) 3, 17, 191, 1424, 1489
MfeI CAATTG 4 cut(s) 108, 526, 969, 1312
MflI RGATCY 1 cut(s) 1493
MlsI TGGCCA 1 cut(s) 1232
MluNI TGGCCA 1 cut(s) 1232
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 3 cut(s) 190, 262, 1267
Mox20I TGGCCA 1 cut(s) 1232
MscI TGGCCA 1 cut(s) 1232
MseI TTAA 5 cut(s) 78, 609, 849, 1203, 1407
Msp20I TGGCCA 1 cut(s) 1232
MspA1I CMGCKG 3 cut(s) 428, 1358, 1499
MunI CAATTG 4 cut(s) 108, 526, 969, 1312
Mva1269I GAATGC 1 cut(s) 539
MwoI GCNNNNNNNGC 5 cut(s) 245, 428, 604, 1127, 1136
NdeI CATATG 1 cut(s) 933
NdeII GATC 2 cut(s) 3, 1493
NlaIII CATG 7 cut(s) 39, 361, 466, 595, 1024, 1117, 1237
NlaIV GGNNCC 4 cut(s) 906, 991, 1173, 1495
NmuCI GTSAC 1 cut(s) 1321
NspI RCATGY 3 cut(s) 361, 466, 1117
NspV TTCGAA 1 cut(s) 692
PaeI GCATGC 1 cut(s) 361
PaeR7I CTCGAG 1 cut(s) 1295
PagI TCATGA 2 cut(s) 591, 1020
PciI ACATGT 2 cut(s) 462, 1113
PcsI WCGNNNNNNNCGW 1 cut(s) 1293
PctI GAATGC 1 cut(s) 539
PfeI GAWTC 4 cut(s) 259, 629, 680, 1423
PflMI CCANNNNNTGG 1 cut(s) 1361
PkrI GCNGC 7 cut(s) 356, 400, 430, 606, 1063, 1360, 1501
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
PpuMI RGGWCCY 1 cut(s) 1172
PscI ACATGT 2 cut(s) 462, 1113
PshAI GACNNNNGTC 1 cut(s) 662
PsiI TTATAA 1 cut(s) 921
Psp5II RGGWCCY 1 cut(s) 1172
PspFI CCCAGC 1 cut(s) 424
PspN4I GGNNCC 4 cut(s) 906, 991, 1173, 1495
PspPI GGNCC 5 cut(s) 97, 339, 905, 1172, 1446
PspPPI RGGWCCY 1 cut(s) 1172
PstNI CAGNNNCTG 1 cut(s) 1361
PsuI RGATCY 1 cut(s) 1493
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 3 cut(s) 872, 893, 991
RsaNI GTAC 3 cut(s) 871, 892, 990
SaqAI TTAA 5 cut(s) 78, 609, 849, 1203, 1407
SatI GCNGC 7 cut(s) 355, 399, 429, 605, 1062, 1359, 1500
Sau3AI GATC 2 cut(s) 3, 1493
Sau96I GGNCC 5 cut(s) 97, 339, 905, 1172, 1446
SchI GAGTC 1 cut(s) 142
SfaNI GCATC 4 cut(s) 176, 552, 931, 1314
Sfr274I CTCGAG 1 cut(s) 1295
SfuI TTCGAA 1 cut(s) 692
SinI GGWCC 4 cut(s) 97, 339, 1172, 1446
SlaI CTCGAG 1 cut(s) 1295
SmlI CTYRAG 2 cut(s) 166, 1295
SmoI CTYRAG 2 cut(s) 166, 1295
SphI GCATGC 1 cut(s) 361
SsiI CCGC 3 cut(s) 355, 1358, 1497
SspI AATATT 4 cut(s) 456, 737, 984, 1083
SspMI CTAG 7 cut(s) 362, 581, 857, 1127, 1209, 1223, 1392
TaaI ACNGT 4 cut(s) 442, 994, 1199, 1243
TaiI ACGT 1 cut(s) 1070
TaqI TCGA 2 cut(s) 692, 1296
TaqII GACCGA 1 cut(s) 114
TauI GCSGC 2 cut(s) 357, 1361
TfiI GAWTC 4 cut(s) 259, 629, 680, 1423
Tru1I TTAA 5 cut(s) 78, 609, 849, 1203, 1407
Tru9I TTAA 5 cut(s) 78, 609, 849, 1203, 1407
TscAI CASTG 3 cut(s) 723, 1099, 1202
TseFI GTSAC 1 cut(s) 1321
TseI GCWGC 5 cut(s) 398, 428, 604, 1061, 1499
Tsp45I GTSAC 1 cut(s) 1321
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 3 cut(s) 723, 1099, 1202
Van91I CCANNNNNTGG 1 cut(s) 1361
VpaK11BI GGWCC 4 cut(s) 97, 339, 1172, 1446
XapI RAATTY 2 cut(s) 792, 1380
XceI RCATGY 3 cut(s) 361, 466, 1117
XhoI CTCGAG 1 cut(s) 1295
XmiI GTMKAC 1 cut(s) 1238
XspI CTAG 7 cut(s) 362, 581, 857, 1127, 1209, 1223, 1392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.