MD05G1050900.v1.1

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001054
Physical Location & Seq
Forward (+)
8830558 .. 8839241
8684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1050900.v1.1.491

Sequence Viewer

Length: 1608 bp
ATGGTTCAAAAGGAAGAAGCAACAAAGTCTTCTGAAGAAGCCAAACCCCGTCTGGCGGAGACAGCCATGGTTATTGCTTCAACAGCAGCTCCCGCTCCTTCCTGGTTCACGCCCAAGAGGTTACTTGTTATCTTTTGCTTGATTAACTTGATAAATTATGTGGACCGAGGAACAATAGCAAGCAATGGTGTCAATGGGAGCCGCACAACTTGTACAGATAGTGGTATATGCACGGCTGGTACCGGGATACAGGGGGAGTTTCACTTGAACAACTTTCAAGATGGAGTTCTATCATCAGCTTTTATGGTTGGACTTCTTATGGCTTCTCCTATATTTGCATCATTAGCAAAGAGTGTAAGTCCCTTTAGGCTCATTGGAGTTGGGTTATCAGTTTGGACCTTTGCGATAGTTGGTTGTGCTTGTTCGTTGGGTTTCTGGTCCATTACTATTTTCCGCATGCTAGTTGGTGTTGGCGAAGCTTCATTTATTAGTCTTGCAGCTCCATTCATTGACGATAATGCCCCAGCTGCTCAGAAAACTGCGTGGCTTGCTATATTCTACATGTGCATACCAAGTGGATATGCACTTGGCTATGTTTATGGTGGGGTGGTTGGAAGTCACACCAATTGGCGTTATGCATTTGTGGGGGAAGCAATCCTTATGCTACCATTTGCTATTCTAGGATTTGTTATGAAGCCTTTGCAGCTGAAAGGTTTTTTTCATCCTGAATCAAAAAAAGCACTTACAGCAGTGGAAACGGCTGTTGTAGAAGTTCAAGGTATTTCGAATGGTAAAGATGCTTCCTATTTGATGAAGAAAGATGTGAGAAATTCTACCTTACAAAAATCTTCCAAGTTAAAGGTTGCATCAAACATAAAGAATGAAATATCAAGGTTTTTGAATGATATGAAGGTGCTGTTGGTGGATAAGGTTTATGTTGTGAATGTTCTAGGTTACATAGCATACAACTTTGTCATAGGTGCATACTCATATTGGGGACCTAAGGCGGGATATAATATATATCATATGAATGATGCAGATACGATATTTGGAGGTATTACAATCATGTCTGGAATATTGGGCACACTAGCGGGAGGCTTTCTTCTGGATTATATGACTAACACTATCTCTAATGCTTTTAAGCTTCTTTCAGCAGTAACGTTAATTGGTGGTGCCTTTTGCTTTGGTGCCTTTTGCTTCAAGAATATGTATGTTTTCCTAGCTCTTTTCGCAATCGGTGAACTACTTGTCTTTGCAACTCAGGGTCCTGTAAATTACATTTGTCTCCACTGTGTTAAGCCTAGTATGAGGCCGCTATCTATGGCTATTTCTACTGTTTCAATTCATCTCTTCGGTGATGTACCTTCCGCACCTCTTGTTGGAGTTCTCCAGGATGCCATTAACAACTGGAGGAAGACTGCTCTTATTCTAACAGCTGTGTTCTTTCCATCAGCTCTAATATGGTTTATGGGAATCTTTCTGCACAGCGTGGATAGATTTAACGAGGAAAGTGAGCATCAGATCACCACAAAAACCGACAGGTCAAGCACAACACCATTGCTTGGAGGGAAGAAGATGGAAACAACTGAATCTGTTGCTGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

536

Amino Acids

57.92

Weight (kDa)

8.54

Isoelectric Point (pI)

29.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 78 - 447 1.5e-29 Major Facilitator Superfamily
OATP PF03137 152 - 395 8.7e-18 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 398 - 466 2.6e-07 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 239
AccB1I GGYRCC 3 cut(s) 239, 1172, 1187
AccB7I CCANNNNNTGG 1 cut(s) 1562
AccBSI CCGCTC 1 cut(s) 95
AciI CCGC 8 cut(s) 56, 93, 202, 454, 1007, 1091, 1313, 1368
AclI AACGTT 1 cut(s) 1160
AcsI RAATTY 1 cut(s) 829
AcuI CTGAAG 1 cut(s) 54
AdeI CACNNNGTG 1 cut(s) 1489
AfaI GTAC 3 cut(s) 214, 241, 1362
AfiI CCNNNNNNNGG 4 cut(s) 55, 1007, 1379, 1562
AflIII ACRYGT 1 cut(s) 561
AgsI TTSAA 8 cut(s) 8, 81, 268, 278, 776, 901, 1201, 1341
AjnI CCWGG 2 cut(s) 101, 1389
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
Alw26I GTCTC 2 cut(s) 53, 1289
AoxI GGCC 1 cut(s) 1310
ApeKI GCWGC 4 cut(s) 86, 497, 527, 703
ApoI RAATTY 1 cut(s) 829
Asp718I GGTACC 1 cut(s) 239
AspS9I GGNCC 5 cut(s) 163, 396, 438, 998, 1265
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 3 cut(s) 1250, 1367, 1516
AsuII TTCGAA 1 cut(s) 785
AvaII GGWCC 5 cut(s) 163, 396, 438, 998, 1265
AxyI CCTNAGG 1 cut(s) 1002
BaeGI GKGCMC 1 cut(s) 1085
BanI GGYRCC 3 cut(s) 239, 1172, 1187
BarI GAAGNNNNNNTAC 2 cut(s) 784, 816
BbsI GAAGAC 2 cut(s) 21, 1421
BbvI GCAGC 4 cut(s) 98, 509, 514, 715
BccI CCATC 3 cut(s) 275, 1456, 1570
BceAI ACGGC 2 cut(s) 249, 774
BciT130I CCWGG 2 cut(s) 103, 1391
BciVI GTATCC 1 cut(s) 240
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 2 cut(s) 53, 1289
BfaI CTAG 6 cut(s) 461, 680, 950, 1088, 1220, 1302
BfuI GTATCC 1 cut(s) 240
BisI GCNGC 6 cut(s) 87, 202, 498, 528, 704, 1313
BlsI GCNGC 6 cut(s) 88, 203, 499, 529, 705, 1314
Bme1390I CCNGG 3 cut(s) 103, 244, 1391
Bme18I GGWCC 5 cut(s) 163, 396, 438, 998, 1265
BmgT120I GGNCC 5 cut(s) 163, 396, 438, 998, 1265
BmiI GGNNCC 6 cut(s) 200, 241, 999, 1174, 1189, 1266
BmrFI CCNGG 3 cut(s) 103, 244, 1391
BmsI GCATC 6 cut(s) 347, 787, 875, 1024, 1384, 1525
BpiI GAAGAC 2 cut(s) 21, 1421
BpmI CTGGAG 2 cut(s) 1373, 1429
Bpu14I TTCGAA 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 66, 166
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 1007, 1379, 1562
Bse1I ACTGG 1 cut(s) 1412
Bse21I CCTNAGG 1 cut(s) 1002
Bse3DI GCAATG 2 cut(s) 190, 1556
BseBI CCWGG 2 cut(s) 103, 1391
BseDI CCNNGG 2 cut(s) 66, 166
BseGI GGATG 2 cut(s) 721, 1399
BseLI CCNNNNNNNGG 4 cut(s) 55, 1007, 1379, 1562
BseMI GCAATG 2 cut(s) 190, 1556
BseMII CTCAG 2 cut(s) 545, 1274
BseNI ACTGG 1 cut(s) 1412
BseSI GKGCMC 1 cut(s) 1085
BseXI GCAGC 4 cut(s) 98, 509, 514, 715
BseYI CCCAGC 1 cut(s) 523
BsgI GTGCAG 1 cut(s) 1466
BshFI GGCC 1 cut(s) 1312
BshNI GGYRCC 3 cut(s) 239, 1172, 1187
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 2 cut(s) 345, 1011
BslI CCNNNNNNNGG 4 cut(s) 55, 1007, 1379, 1562
BsmAI GTCTC 2 cut(s) 53, 1289
BsmFI GGGAC 2 cut(s) 345, 1011
BsnI GGCC 1 cut(s) 1312
Bsp119I TTCGAA 1 cut(s) 785
Bsp1286I GDGCHC 1 cut(s) 1085
Bsp1407I TGTACA 1 cut(s) 212
Bsp143I GATC 1 cut(s) 1521
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 8 cut(s) 56, 93, 202, 454, 1007, 1091, 1313, 1368
BspANI GGCC 1 cut(s) 1312
BspCNI CTCAG 2 cut(s) 544, 1273
BspLI GGNNCC 6 cut(s) 200, 241, 999, 1174, 1189, 1266
BspT104I TTCGAA 1 cut(s) 785
BspT107I GGYRCC 3 cut(s) 239, 1172, 1187
BsrBI CCGCTC 1 cut(s) 95
BsrDI GCAATG 2 cut(s) 190, 1556
BsrGI TGTACA 1 cut(s) 212
BsrI ACTGG 1 cut(s) 1412
BssECI CCNNGG 2 cut(s) 66, 166
BssMI GATC 1 cut(s) 1521
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 2 cut(s) 103, 1391
Bst4CI ACNGT 2 cut(s) 1292, 1336
Bst6I CTCTTC 1 cut(s) 1355
BstAUI TGTACA 1 cut(s) 212
BstBI TTCGAA 1 cut(s) 785
BstC8I GCNNGC 3 cut(s) 181, 458, 549
BstDEI CTNAG 3 cut(s) 531, 1002, 1260
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 721, 1399
BstKTI GATC 1 cut(s) 1524
BstMAI GTCTC 2 cut(s) 53, 1289
BstMBI GATC 1 cut(s) 1521
BstMWI GCNNNNNNNGC 9 cut(s) 62, 83, 92, 344, 527, 548, 703, 746, 1229
BstNI CCWGG 2 cut(s) 103, 1391
BstNSI RCATGY 2 cut(s) 460, 565
BstSCI CCNGG 3 cut(s) 101, 242, 1389
BstSLI GKGCMC 1 cut(s) 1085
BstV1I GCAGC 4 cut(s) 98, 509, 514, 715
BstV2I GAAGAC 2 cut(s) 21, 1421
Bsu36I CCTNAGG 1 cut(s) 1002
BsuI GTATCC 1 cut(s) 240
BsuRI GGCC 1 cut(s) 1312
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 721, 1399
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 2 cut(s) 756, 1288
Cac8I GCNNGC 3 cut(s) 181, 458, 549
Cfr13I GGNCC 5 cut(s) 163, 396, 438, 998, 1265
Csp6I GTAC 3 cut(s) 213, 240, 1361
CviAII CATG 4 cut(s) 67, 457, 562, 1066
CviQI GTAC 3 cut(s) 213, 240, 1361
DdeI CTNAG 3 cut(s) 531, 1002, 1260
DpnI GATC 1 cut(s) 1523
DpnII GATC 1 cut(s) 1521
DraIII CACNNNGTG 1 cut(s) 1489
Eam1104I CTCTTC 1 cut(s) 1355
EarI CTCTTC 1 cut(s) 1355
EciI GGCGGA 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 66
Eco47I GGWCC 5 cut(s) 163, 396, 438, 998, 1265
Eco57I CTGAAG 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 1002
EcoO109I RGGNCCY 2 cut(s) 998, 1265
EcoRII CCWGG 2 cut(s) 101, 1389
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 640
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 4 cut(s) 70, 460, 565, 1069
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 2 cut(s) 345, 1011
FatI CATG 4 cut(s) 66, 456, 561, 1065
FauI CCCGC 3 cut(s) 100, 1000, 1084
FauNDI CATATG 1 cut(s) 1026
Fnu4HI GCNGC 6 cut(s) 87, 202, 498, 528, 704, 1313
FokI GGATG 2 cut(s) 708, 1406
Fsp4HI GCNGC 6 cut(s) 87, 202, 498, 528, 704, 1313
FspBI CTAG 6 cut(s) 461, 680, 950, 1088, 1220, 1302
GluI GCNGC 6 cut(s) 87, 202, 498, 528, 704, 1313
GsaI CCCAGC 1 cut(s) 527
GsuI CTGGAG 2 cut(s) 1373, 1429
HaeIII GGCC 1 cut(s) 1312
HapII CCGG 1 cut(s) 243
Hin1II CATG 4 cut(s) 70, 460, 565, 1069
HindIII AAGCTT 2 cut(s) 477, 1142
HinfI GANTC 3 cut(s) 728, 1473, 1589
HpaII CCGG 1 cut(s) 243
HphI GGTGA 3 cut(s) 1250, 1367, 1516
Hpy166II GTNNAC 3 cut(s) 108, 163, 1241
Hpy188I TCNGA 3 cut(s) 34, 534, 1521
Hpy188III TCNNGA 5 cut(s) 278, 725, 1071, 1106, 1201
Hpy8I GTNNAC 3 cut(s) 108, 163, 1241
HpyAV CCTTC 3 cut(s) 108, 904, 1374
HpyCH4III ACNGT 2 cut(s) 1292, 1336
HpyCH4IV ACGT 1 cut(s) 1160
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 83, 92, 344, 527, 548, 703, 746, 1229
HpyF3I CTNAG 3 cut(s) 531, 1002, 1260
HpySE526I ACGT 1 cut(s) 1160
Hsp92II CATG 4 cut(s) 70, 460, 565, 1069
KpnI GGTACC 1 cut(s) 243
Kzo9I GATC 1 cut(s) 1521
LmnI GCTCC 4 cut(s) 94, 100, 198, 505
Lsp1109I GCAGC 4 cut(s) 98, 509, 514, 715
LweI GCATC 6 cut(s) 347, 787, 875, 1024, 1384, 1525
MaeI CTAG 6 cut(s) 461, 680, 950, 1088, 1220, 1302
MaeII ACGT 1 cut(s) 1160
MaeIII GTNAC 4 cut(s) 120, 617, 953, 1156
MalI GATC 1 cut(s) 1523
MbiI CCGCTC 1 cut(s) 95
MboI GATC 1 cut(s) 1521
MfeI CAATTG 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 1085
MluCI AATT 6 cut(s) 154, 625, 829, 1164, 1273, 1341
MmeI TCCRAC 3 cut(s) 289, 592, 1360
MnlI CCTC 9 cut(s) 111, 161, 1046, 1088, 1302, 1383, 1404, 1498, 1559
Mph1103I ATGCAT 1 cut(s) 640
MseI TTAA 7 cut(s) 144, 857, 1140, 1163, 1296, 1401, 1500
MslI CAYNNNNRTG 1 cut(s) 1029
MspA1I CMGCKG 3 cut(s) 527, 706, 1436
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 3 cut(s) 103, 244, 1391
MunI CAATTG 1 cut(s) 625
MvaI CCWGG 2 cut(s) 103, 1391
MwoI GCNNNNNNNGC 9 cut(s) 62, 83, 92, 344, 527, 548, 703, 746, 1229
NciI CCSGG 1 cut(s) 244
NcoI CCATGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 1026
NdeII GATC 1 cut(s) 1521
NlaIII CATG 4 cut(s) 70, 460, 565, 1069
NlaIV GGNNCC 6 cut(s) 200, 241, 999, 1174, 1189, 1266
NmuCI GTSAC 1 cut(s) 617
NsiI ATGCAT 1 cut(s) 640
NspI RCATGY 2 cut(s) 460, 565
NspV TTCGAA 1 cut(s) 785
PaeI GCATGC 1 cut(s) 460
PciI ACATGT 1 cut(s) 561
PfeI GAWTC 3 cut(s) 728, 1473, 1589
PflMI CCANNNNNTGG 1 cut(s) 1562
PfoI TCCNGGA 1 cut(s) 1389
PkrI GCNGC 6 cut(s) 88, 203, 499, 529, 705, 1314
PpuMI RGGWCCY 2 cut(s) 998, 1265
PscI ACATGT 1 cut(s) 561
Psp1406I AACGTT 1 cut(s) 1160
Psp5II RGGWCCY 2 cut(s) 998, 1265
Psp6I CCWGG 2 cut(s) 101, 1389
PspFI CCCAGC 1 cut(s) 523
PspGI CCWGG 2 cut(s) 101, 1389
PspN4I GGNNCC 6 cut(s) 200, 241, 999, 1174, 1189, 1266
PspPI GGNCC 5 cut(s) 163, 396, 438, 998, 1265
PspPPI RGGWCCY 2 cut(s) 998, 1265
PvuII CAGCTG 3 cut(s) 527, 706, 1436
RsaI GTAC 3 cut(s) 214, 241, 1362
RsaNI GTAC 3 cut(s) 213, 240, 1361
RseI CAYNNNNRTG 1 cut(s) 1029
SaqAI TTAA 7 cut(s) 144, 857, 1140, 1163, 1296, 1401, 1500
SatI GCNGC 6 cut(s) 87, 202, 498, 528, 704, 1313
Sau3AI GATC 1 cut(s) 1521
Sau96I GGNCC 5 cut(s) 163, 396, 438, 998, 1265
ScrFI CCNGG 3 cut(s) 103, 244, 1391
SduI GDGCHC 1 cut(s) 1085
SfaNI GCATC 6 cut(s) 347, 787, 875, 1024, 1384, 1525
SfuI TTCGAA 1 cut(s) 785
SinI GGWCC 5 cut(s) 163, 396, 438, 998, 1265
SmiMI CAYNNNNRTG 1 cut(s) 1029
SphI GCATGC 1 cut(s) 460
Sse9I AATT 6 cut(s) 154, 625, 829, 1164, 1273, 1341
SsiI CCGC 8 cut(s) 56, 93, 202, 454, 1007, 1091, 1313, 1368
SspI AATATT 1 cut(s) 1077
SspMI CTAG 6 cut(s) 461, 680, 950, 1088, 1220, 1302
StyD4I CCNGG 3 cut(s) 101, 242, 1389
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 2 cut(s) 1292, 1336
TaiI ACGT 1 cut(s) 1163
TaqI TCGA 1 cut(s) 785
TaqII GACCGA 1 cut(s) 180
TasI AATT 6 cut(s) 154, 625, 829, 1164, 1273, 1341
TatI WGTACW 1 cut(s) 212
TauI GCSGC 2 cut(s) 204, 1315
TfiI GAWTC 3 cut(s) 728, 1473, 1589
Tru1I TTAA 7 cut(s) 144, 857, 1140, 1163, 1296, 1401, 1500
Tru9I TTAA 7 cut(s) 144, 857, 1140, 1163, 1296, 1401, 1500
TscAI CASTG 2 cut(s) 756, 1295
TseFI GTSAC 1 cut(s) 617
TseI GCWGC 4 cut(s) 86, 497, 527, 703
Tsp45I GTSAC 1 cut(s) 617
TspDTI ATGAA 9 cut(s) 471, 496, 707, 710, 827, 897, 923, 1043, 1334
TspRI CASTG 2 cut(s) 756, 1295
Van91I CCANNNNNTGG 1 cut(s) 1562
VpaK11BI GGWCC 5 cut(s) 163, 396, 438, 998, 1265
XapI RAATTY 1 cut(s) 829
XceI RCATGY 2 cut(s) 460, 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XspI CTAG 6 cut(s) 461, 680, 950, 1088, 1220, 1302
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.