Prupe.8G077700_v2.0.a1

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001054
Physical Location & Seq
Forward (+)
11181030 .. 11190565
9536 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G077700.1

Sequence Viewer

Length: 1602 bp
ATGGCTCAAAAAGAAGCAAAGACTTCTGAAGAAGTTAAGCCTTCGGCAGAGCCAGCCATGGTCATTGCTTCAACCGCAGCTCCGGCTCCTTCGTGGTTCACGCCCAAGAGGTTACTTGCTATCTTTTGCGTGATTAACTTATTAAATTATGTGGACCGAGGAGCAATAGCAAGCAATGGTGTCAATGGGAGCCTCGGAACTTGTACAGAAAGTGGCGTATGCACATCCGGTACCGGGATACAGGGGGATTTTAACTTGAGCAACTTTGAAGATGGAGTTCTCTCATCAGCTTTTATGGTTGGACTTCTTTTGGCTTCTCCTATATTTGCATCATTAGCAAAGAGTGTAAGTCCGTTTAGGCTCATTGGAGTTGGATTATCAGTTTGGACCTTTGCTACAATTGGTTGTGGTTTTTCTTTCAACTTCTGGTCCATTACTCTCTGCCGCATGTTAGTTGGTGTTGGTGAGGCTTCATTTATTAGTCTTGCAGCTCCATTCATTGATGATAATGCCCCAGCTCCTCAGAAAACTGCGTGGCTTGCTATATTCTATATGTGCATACCAAGTGGATATGCACTTGGCTATGTTTATGGTGGGTTGGTTGGAAGTCATTCCAATTGGCGTTATGCTTTTTGGGGGGAGGCAATCCTGATGCTTCCATTTGCTATTCTAGGATTTGTTATGAAGCCCTTGCAGCTGAAAGGTTTTTTTCATCCTGAATCAACAAAAGCACTAACAGCAGTGGAGACGGCTGTCTCAGAAGTTCAAGGTTCAGATAATTTGAATGGTAAAGATGGTTCCTTTTCCATTAAGGAAGATCTCAGAGATTCTACCATACAGAAATCTTCTAAGCTAAAAGTTGGGACAAAAATAATGAATCAAATTTCAAGGTTTATGAAAGATATGAAGGTGCTTTTGGTTGACAAGGTTTATGTTGTTAATGTTCTAGGTTATATAGCATACAACTTTGTCATAGGTGCATACTCGTATTGGGGGCCTAAGGCTGGTTATAATATATATCATATGAATGATGCAGATTTGATATTTGGAGGTATTACAATTGTGTGTGGAATATTGGGCACACTAGCAGGAGGTTTTGTTCTGGATTATATCAGTAGCACTATCTCTAATGCTTTCAAGCTTCTTTCAGCAGTAACGTTACTTGGAGGAGCATGTTGCTTCGGTGCCTTTTGCTTCAAGAACATGTATGCTTTCCTAGCTCTTTTTGCAGTTGGGGAACTACTTGTCTTTGCAACTCAGGGCCCTGTAAATTACATTTGTCTCCATTGTGTTAAACCTAGTATGAGGCCACTATCTATGGCTATTTCTACTGTTTCAATTCACCTTTTTGGGGATGTACCTTCCGCACCTCTTGTTGGAGTTCTCCAGGATTCCATAAACAACTGGAGGGTGACTGCTCTTATTTTAACATCTATTTTCATTCCAGCAGCAGGAATATGGTTTATAGGAATCTTTCTGCACAGCGTGGATAGATTTAACGAAGAAAGTGAGAATCAGATCACGACAACTGAAAGGTCAAACACAACACCATTGCTTAAAGGGAAGAAGACAGAAACAACTAAATCTGCCTCTGAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

534

Amino Acids

57.31

Weight (kDa)

7.47

Isoelectric Point (pI)

27.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1011
Acc65I GGTACC 1 cut(s) 230
AccB1I GGYRCC 2 cut(s) 230, 1184
AciI CCGC 3 cut(s) 75, 445, 1365
AclI AACGTT 1 cut(s) 1157
AcsI RAATTY 1 cut(s) 882
AcuI CTGAAG 1 cut(s) 48
AdeI CACNNNGTG 1 cut(s) 1486
AfaI GTAC 3 cut(s) 205, 232, 1359
AfiI CCNNNNNNNGG 5 cut(s) 234, 1004, 1351, 1376, 1451
AflIII ACRYGT 1 cut(s) 1203
AgsI TTSAA 9 cut(s) 72, 269, 421, 767, 784, 888, 1138, 1198, 1338
AjnI CCWGG 1 cut(s) 1386
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AloI GAACNNNNNNTCC 2 cut(s) 1083, 1115
AluBI AGCT 8 cut(s) 80, 290, 491, 518, 697, 853, 1141, 1220
AluI AGCT 8 cut(s) 80, 290, 491, 518, 697, 853, 1141, 1220
Alw26I GTCTC 3 cut(s) 740, 760, 1286
AoxI GGCC 3 cut(s) 995, 1261, 1307
ApaI GGGCCC 1 cut(s) 1265
ApeKI GCWGC 4 cut(s) 77, 488, 694, 1448
ApoI RAATTY 1 cut(s) 882
Asp700I GAANNNNTTC 1 cut(s) 610
Asp718I GGTACC 1 cut(s) 230
AspS9I GGNCC 6 cut(s) 154, 387, 429, 995, 1261, 1262
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 3 cut(s) 476, 1334, 1423
AvaII GGWCC 3 cut(s) 154, 387, 429
AxyI CCTNAGG 1 cut(s) 999
BaeGI GKGCMC 2 cut(s) 1082, 1265
BanI GGYRCC 2 cut(s) 230, 1184
BanII GRGCYC 1 cut(s) 1265
BbsI GAAGAC 1 cut(s) 1574
BbvI GCAGC 4 cut(s) 89, 500, 706, 1460
BccI CCATC 2 cut(s) 266, 788
BceAI ACGGC 1 cut(s) 765
BciT130I CCWGG 1 cut(s) 1388
BciVI GTATCC 1 cut(s) 231
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 3 cut(s) 740, 760, 1286
BfaI CTAG 5 cut(s) 671, 947, 1085, 1217, 1299
BfuI GTATCC 1 cut(s) 231
BglII AGATCT 1 cut(s) 817
BisI GCNGC 5 cut(s) 78, 445, 489, 695, 1449
BlsI GCNGC 5 cut(s) 79, 446, 490, 696, 1450
Bme1390I CCNGG 2 cut(s) 235, 1388
Bme18I GGWCC 3 cut(s) 154, 387, 429
BmgT120I GGNCC 6 cut(s) 154, 387, 429, 995, 1261, 1262
BmiI GGNNCC 7 cut(s) 87, 191, 232, 799, 996, 1186, 1263
BmrFI CCNGG 2 cut(s) 235, 1388
BmsI GCATC 3 cut(s) 338, 642, 1021
BoxI GACNNNNGTC 1 cut(s) 752
BpiI GAAGAC 1 cut(s) 1574
BpmI CTGGAG 2 cut(s) 1370, 1426
BpuEI CTTGAG 1 cut(s) 277
BpuMI CCSGG 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 57, 157, 193
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 1083, 1113
Bsc4I CCNNNNNNNGG 5 cut(s) 234, 1004, 1351, 1376, 1451
Bse1I ACTGG 1 cut(s) 1409
Bse21I CCTNAGG 1 cut(s) 999
Bse3DI GCAATG 3 cut(s) 63, 181, 1550
BseBI CCWGG 1 cut(s) 1388
BseDI CCNNGG 3 cut(s) 57, 157, 193
BseGI GGATG 3 cut(s) 224, 712, 1360
BseLI CCNNNNNNNGG 5 cut(s) 234, 1004, 1351, 1376, 1451
BseMI GCAATG 3 cut(s) 63, 181, 1550
BseMII CTCAG 4 cut(s) 536, 771, 835, 1271
BseNI ACTGG 1 cut(s) 1409
BseRI GAGGAG 3 cut(s) 174, 510, 1182
BseSI GKGCMC 2 cut(s) 1082, 1265
BseXI GCAGC 4 cut(s) 89, 500, 706, 1460
BseYI CCCAGC 1 cut(s) 514
BsgI GTGCAG 1 cut(s) 1463
BshFI GGCC 3 cut(s) 997, 1263, 1309
BshNI GGYRCC 2 cut(s) 230, 1184
BsiSI CCGG 3 cut(s) 83, 228, 234
BslFI GGGAC 1 cut(s) 877
BslI CCNNNNNNNGG 5 cut(s) 234, 1004, 1351, 1376, 1451
BsmAI GTCTC 3 cut(s) 740, 760, 1286
BsmBI CGTCTC 1 cut(s) 740
BsmFI GGGAC 1 cut(s) 877
BsnI GGCC 3 cut(s) 997, 1263, 1309
Bsp120I GGGCCC 1 cut(s) 1261
Bsp1286I GDGCHC 2 cut(s) 1082, 1265
Bsp1407I TGTACA 1 cut(s) 203
Bsp143I GATC 2 cut(s) 817, 1518
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 3 cut(s) 75, 445, 1365
BspANI GGCC 3 cut(s) 997, 1263, 1309
BspCNI CTCAG 4 cut(s) 535, 770, 834, 1270
BspLI GGNNCC 7 cut(s) 87, 191, 232, 799, 996, 1186, 1263
BspT107I GGYRCC 2 cut(s) 230, 1184
BsrDI GCAATG 3 cut(s) 63, 181, 1550
BsrGI TGTACA 1 cut(s) 203
BsrI ACTGG 1 cut(s) 1409
BssECI CCNNGG 3 cut(s) 57, 157, 193
BssMI GATC 2 cut(s) 817, 1518
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 1388
Bst4CI ACNGT 1 cut(s) 1333
BstAUI TGTACA 1 cut(s) 203
BstC8I GCNNGC 3 cut(s) 54, 172, 540
BstDEI CTNAG 6 cut(s) 522, 757, 821, 849, 999, 1257
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 3 cut(s) 224, 712, 1360
BstKTI GATC 2 cut(s) 820, 1521
BstMAI GTCTC 3 cut(s) 740, 760, 1286
BstMBI GATC 2 cut(s) 817, 1518
BstMWI GCNNNNNNNGC 9 cut(s) 53, 74, 83, 335, 539, 694, 737, 1217, 1226
BstNI CCWGG 1 cut(s) 1388
BstNSI RCATGY 3 cut(s) 451, 1176, 1207
BstPAI GACNNNNGTC 1 cut(s) 752
BstSCI CCNGG 2 cut(s) 233, 1386
BstSLI GKGCMC 2 cut(s) 1082, 1265
BstV1I GCAGC 4 cut(s) 89, 500, 706, 1460
BstV2I GAAGAC 1 cut(s) 1574
BstX2I RGATCY 1 cut(s) 817
BstYI RGATCY 1 cut(s) 817
Bsu36I CCTNAGG 1 cut(s) 999
BsuI GTATCC 1 cut(s) 231
BsuRI GGCC 3 cut(s) 997, 1263, 1309
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 3 cut(s) 224, 712, 1360
BtsI GCAGTG 1 cut(s) 747
BtsIMutI CAGTG 1 cut(s) 747
Cac8I GCNNGC 3 cut(s) 54, 172, 540
Cfr13I GGNCC 6 cut(s) 154, 387, 429, 995, 1261, 1262
Csp6I GTAC 3 cut(s) 204, 231, 1358
CviAII CATG 4 cut(s) 58, 448, 1173, 1204
CviQI GTAC 3 cut(s) 204, 231, 1358
DdeI CTNAG 6 cut(s) 522, 757, 821, 849, 999, 1257
DpnI GATC 2 cut(s) 819, 1520
DpnII GATC 2 cut(s) 817, 1518
DraIII CACNNNGTG 1 cut(s) 1486
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 1265
Eco47I GGWCC 3 cut(s) 154, 387, 429
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 999
EcoO109I RGGNCCY 3 cut(s) 995, 1261, 1262
EcoRII CCWGG 1 cut(s) 1386
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 1265
ErhI CCWWGG 1 cut(s) 57
Esp3I CGTCTC 1 cut(s) 740
FaeI CATG 4 cut(s) 61, 451, 1176, 1207
FaqI GGGAC 1 cut(s) 877
FatI CATG 4 cut(s) 57, 447, 1172, 1203
FauNDI CATATG 1 cut(s) 1023
Fnu4HI GCNGC 5 cut(s) 78, 445, 489, 695, 1449
FokI GGATG 3 cut(s) 211, 699, 1367
FriOI GRGCYC 1 cut(s) 1265
Fsp4HI GCNGC 5 cut(s) 78, 445, 489, 695, 1449
FspBI CTAG 5 cut(s) 671, 947, 1085, 1217, 1299
GluI GCNGC 5 cut(s) 78, 445, 489, 695, 1449
GsaI CCCAGC 1 cut(s) 518
GsuI CTGGAG 2 cut(s) 1370, 1426
HaeIII GGCC 3 cut(s) 997, 1263, 1309
HapII CCGG 3 cut(s) 83, 228, 234
Hin1II CATG 4 cut(s) 61, 451, 1176, 1207
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HindIII AAGCTT 1 cut(s) 1139
HinfI GANTC 6 cut(s) 719, 827, 877, 1391, 1470, 1513
HpaII CCGG 3 cut(s) 83, 228, 234
HphI GGTGA 3 cut(s) 476, 1334, 1423
Hpy166II GTNNAC 3 cut(s) 99, 154, 922
Hpy188I TCNGA 8 cut(s) 28, 197, 525, 760, 775, 824, 1518, 1594
Hpy188III TCNNGA 5 cut(s) 649, 716, 1103, 1198, 1522
Hpy8I GTNNAC 3 cut(s) 99, 154, 922
HpyAV CCTTC 4 cut(s) 51, 99, 901, 1371
HpyCH4III ACNGT 1 cut(s) 1333
HpyCH4IV ACGT 1 cut(s) 1157
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 74, 83, 335, 539, 694, 737, 1217, 1226
HpyF3I CTNAG 6 cut(s) 522, 757, 821, 849, 999, 1257
HpySE526I ACGT 1 cut(s) 1157
Hsp92II CATG 4 cut(s) 61, 451, 1176, 1207
KpnI GGTACC 1 cut(s) 234
Kzo9I GATC 2 cut(s) 817, 1518
LmnI GCTCC 7 cut(s) 85, 91, 161, 189, 496, 523, 1169
Lsp1109I GCAGC 4 cut(s) 89, 500, 706, 1460
LweI GCATC 3 cut(s) 338, 642, 1021
MaeI CTAG 5 cut(s) 671, 947, 1085, 1217, 1299
MaeII ACGT 1 cut(s) 1157
MaeIII GTNAC 4 cut(s) 111, 1153, 1158, 1411
MalI GATC 2 cut(s) 819, 1520
MboI GATC 2 cut(s) 817, 1518
MboII GAAGA 7 cut(s) 41, 281, 827, 837, 1514, 1576, 1579
MfeI CAATTG 3 cut(s) 399, 616, 1059
MflI RGATCY 1 cut(s) 817
MhlI GDGCHC 2 cut(s) 1082, 1265
MluCI AATT 8 cut(s) 145, 399, 616, 778, 882, 1059, 1270, 1338
MmeI TCCRAC 4 cut(s) 280, 352, 583, 1357
MroXI GAANNNNTTC 1 cut(s) 610
MslI CAYNNNNRTG 1 cut(s) 1026
MspA1I CMGCKG 1 cut(s) 697
MspI CCGG 3 cut(s) 83, 228, 234
MspR9I CCNGG 2 cut(s) 235, 1388
MunI CAATTG 3 cut(s) 399, 616, 1059
MvaI CCWGG 1 cut(s) 1388
MwoI GCNNNNNNNGC 9 cut(s) 53, 74, 83, 335, 539, 694, 737, 1217, 1226
NciI CCSGG 1 cut(s) 235
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 1023
NdeII GATC 2 cut(s) 817, 1518
NlaIII CATG 4 cut(s) 61, 451, 1176, 1207
NlaIV GGNNCC 7 cut(s) 87, 191, 232, 799, 996, 1186, 1263
NmuCI GTSAC 1 cut(s) 1411
NspI RCATGY 3 cut(s) 451, 1176, 1207
PciI ACATGT 1 cut(s) 1203
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 6 cut(s) 719, 827, 877, 1391, 1470, 1513
PfoI TCCNGGA 1 cut(s) 1386
PkrI GCNGC 5 cut(s) 79, 446, 490, 696, 1450
PscI ACATGT 1 cut(s) 1203
PshAI GACNNNNGTC 1 cut(s) 752
PsiI TTATAA 1 cut(s) 1011
Psp1406I AACGTT 1 cut(s) 1157
Psp6I CCWGG 1 cut(s) 1386
PspFI CCCAGC 1 cut(s) 514
PspGI CCWGG 1 cut(s) 1386
PspN4I GGNNCC 7 cut(s) 87, 191, 232, 799, 996, 1186, 1263
PspOMI GGGCCC 1 cut(s) 1261
PspPI GGNCC 6 cut(s) 154, 387, 429, 995, 1261, 1262
PsrI GAACNNNNNNTAC 2 cut(s) 781, 813
PsuI RGATCY 1 cut(s) 817
PvuII CAGCTG 1 cut(s) 697
RsaI GTAC 3 cut(s) 205, 232, 1359
RsaNI GTAC 3 cut(s) 204, 231, 1358
RseI CAYNNNNRTG 1 cut(s) 1026
SatI GCNGC 5 cut(s) 78, 445, 489, 695, 1449
Sau3AI GATC 2 cut(s) 817, 1518
Sau96I GGNCC 6 cut(s) 154, 387, 429, 995, 1261, 1262
ScrFI CCNGG 2 cut(s) 235, 1388
SduI GDGCHC 2 cut(s) 1082, 1265
SfaNI GCATC 3 cut(s) 338, 642, 1021
SinI GGWCC 3 cut(s) 154, 387, 429
SmiMI CAYNNNNRTG 1 cut(s) 1026
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 8 cut(s) 145, 399, 616, 778, 882, 1059, 1270, 1338
SsiI CCGC 3 cut(s) 75, 445, 1365
SspI AATATT 1 cut(s) 1074
SspMI CTAG 5 cut(s) 671, 947, 1085, 1217, 1299
StyD4I CCNGG 2 cut(s) 233, 1386
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 1333
TaiI ACGT 1 cut(s) 1160
TaqII GACCGA 1 cut(s) 171
TasI AATT 8 cut(s) 145, 399, 616, 778, 882, 1059, 1270, 1338
TatI WGTACW 1 cut(s) 203
TauI GCSGC 1 cut(s) 447
TfiI GAWTC 6 cut(s) 719, 827, 877, 1391, 1470, 1513
TscAI CASTG 1 cut(s) 747
TseFI GTSAC 1 cut(s) 1411
TseI GCWGC 4 cut(s) 77, 488, 694, 1448
Tsp45I GTSAC 1 cut(s) 1411
TspDTI ATGAA 9 cut(s) 462, 487, 698, 701, 890, 911, 920, 1040, 1429
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 1 cut(s) 747
VpaK11BI GGWCC 3 cut(s) 154, 387, 429
XapI RAATTY 1 cut(s) 882
XceI RCATGY 3 cut(s) 451, 1176, 1207
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 5 cut(s) 671, 947, 1085, 1217, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.