Rroxscaffold_3G00252830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001054
Physical Location & Seq
Reverse (-)
46880661 .. 46898206
17546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252830.1

Sequence Viewer

Length: 624 bp
ATGGACCCGAAGAGATTAAGCCCACATGGTCTAGGCCCAGGCCCGAGGCCTAAAATCAAACAAAAAAGCTGGCCGAGCACCACTCCGCCGCCGCCTTCCGCCGCCGCCGCCCGGTCCGGGGACGTCCAACCAGGCGACCATCTCTACACCCATCAAAAGCAGCACCACACCATCATGCCGGTGCTAAGCCCAACCACAAAAGATCAATACATCGAAATTCCCAAACTTTCTCTTTGCCTTTGTTCTATGGCTCATAGGATCACCCTCCCAATTTATCCCAAAGATCCAACGGTTCGACGCTCGGAGGCCGAGGCGTGGAGTTCGCTGTCCTCATCTCCTCTCTTGTCCTGCTGCACCCGGTGCTTTGTCGTTGAGGGTGGGATCGGTGTTGGGTGGACCCATGGTGCTGCGATGGGTCGGGCGGCCGAGCCACCATGGGTAGGTGAAGGAATGGAGCGGGGCGGTGTTTGGGCGGTTGTCCTCTTGGGTCCGGATTCACCATCCGGATCCGGGCTGTGTGGGGTTGGGATCATGGAGTACTTGGATGGCGTCCTCCTATGGTGGTCCGATGGCGGCGACGAGCGGCGGTATTGGAGGCCCGTCATCGGAGGCGTGATGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

22.31

Weight (kDa)

8.23

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
AccBSI CCGCTC 2 cut(s) 457, 583
AccIII TCCGGA 2 cut(s) 490, 503
AclWI GGATC 6 cut(s) 266, 278, 389, 501, 514, 536
AcoI YGGCCR 2 cut(s) 71, 423
AcsI RAATTY 1 cut(s) 216
AcyI GRCGYC 2 cut(s) 123, 549
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 7 cut(s) 111, 117, 118, 315, 440, 510, 605
AjnI CCWGG 2 cut(s) 37, 130
AluBI AGCT 1 cut(s) 69
AluI AGCT 1 cut(s) 69
Alw21I GWGCWC 1 cut(s) 80
AlwI GGATC 6 cut(s) 266, 278, 389, 501, 514, 536
Ama87I CYCGRG 1 cut(s) 43
Aor13HI TCCGGA 2 cut(s) 490, 503
AoxI GGCC 7 cut(s) 34, 40, 47, 71, 306, 423, 596
ApeKI GCWGC 3 cut(s) 160, 351, 407
ApoI RAATTY 1 cut(s) 216
AspS9I GGNCC 8 cut(s) 4, 35, 41, 114, 396, 488, 564, 597
AsuC2I CCSGG 4 cut(s) 112, 118, 358, 511
AsuHPI GGTGA 3 cut(s) 253, 455, 489
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 5 cut(s) 4, 114, 396, 488, 564
BamHI GGATCC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 3 cut(s) 172, 338, 394
BccI CCATC 8 cut(s) 147, 159, 179, 406, 508, 539, 563, 610
BciT130I CCWGG 2 cut(s) 39, 132
BcnI CCSGG 4 cut(s) 112, 118, 358, 511
BfaI CTAG 1 cut(s) 32
BlpI GCTNAGC 1 cut(s) 185
BmcAI AGTACT 1 cut(s) 539
Bme1390I CCNGG 6 cut(s) 39, 112, 118, 132, 358, 511
Bme18I GGWCC 5 cut(s) 4, 114, 396, 488, 564
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 8 cut(s) 4, 35, 41, 114, 396, 488, 564, 597
BmiI GGNNCC 4 cut(s) 6, 398, 489, 508
BmrFI CCNGG 6 cut(s) 39, 112, 118, 132, 358, 511
BplI GAGNNNNNCTC 2 cut(s) 67, 99
Bpu1102I GCTNAGC 1 cut(s) 185
BpuMI CCSGG 4 cut(s) 112, 118, 358, 511
BsaHI GRCGYC 2 cut(s) 123, 549
BsaJI CCNNGG 6 cut(s) 37, 44, 117, 309, 400, 434
BsaWI WCCGGW 2 cut(s) 490, 503
Bsc4I CCNNNNNNNGG 7 cut(s) 111, 117, 118, 315, 440, 510, 605
Bse118I RCCGGY 1 cut(s) 178
BseAI TCCGGA 2 cut(s) 490, 503
BseBI CCWGG 2 cut(s) 39, 132
BseDI CCNNGG 6 cut(s) 37, 44, 117, 309, 400, 434
BseGI GGATG 2 cut(s) 500, 550
BseLI CCNNNNNNNGG 7 cut(s) 111, 117, 118, 315, 440, 510, 605
BseRI GAGGAG 1 cut(s) 327
BseX3I CGGCCG 1 cut(s) 423
BseXI GCAGC 3 cut(s) 172, 338, 394
BsgI GTGCAG 1 cut(s) 337
Bsh1285I CGRYCG 1 cut(s) 426
BshFI GGCC 7 cut(s) 36, 42, 49, 73, 308, 425, 598
BsiEI CGRYCG 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 7 cut(s) 112, 117, 179, 358, 491, 504, 510
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 7 cut(s) 111, 117, 118, 315, 440, 510, 605
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 7 cut(s) 36, 42, 49, 73, 308, 425, 598
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 80
Bsp13I TCCGGA 2 cut(s) 490, 503
Bsp143I GATC 6 cut(s) 202, 258, 283, 381, 506, 528
Bsp1720I GCTNAGC 1 cut(s) 185
Bsp19I CCATGG 2 cut(s) 400, 434
BspANI GGCC 7 cut(s) 36, 42, 49, 73, 308, 425, 598
BspEI TCCGGA 2 cut(s) 490, 503
BspLI GGNNCC 4 cut(s) 6, 398, 489, 508
BspPI GGATC 6 cut(s) 266, 278, 389, 501, 514, 536
BsrBI CCGCTC 2 cut(s) 457, 583
BsrFI RCCGGY 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 6 cut(s) 37, 44, 117, 309, 400, 434
BssMI GATC 6 cut(s) 202, 258, 283, 381, 506, 528
BssNI GRCGYC 2 cut(s) 123, 549
BssT1I CCWWGG 2 cut(s) 400, 434
Bst2UI CCWGG 2 cut(s) 39, 132
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 2 cut(s) 123, 549
BstAPI GCANNNNNTGC 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 1 cut(s) 185
BstDSI CCRYGG 2 cut(s) 400, 434
BstF5I GGATG 2 cut(s) 500, 550
BstKTI GATC 6 cut(s) 205, 261, 286, 384, 509, 531
BstMBI GATC 6 cut(s) 202, 258, 283, 381, 506, 528
BstMCI CGRYCG 1 cut(s) 426
BstMWI GCNNNNNNNGC 3 cut(s) 75, 107, 360
BstNI CCWGG 2 cut(s) 39, 132
BstSCI CCNGG 6 cut(s) 37, 110, 116, 130, 356, 509
BstV1I GCAGC 3 cut(s) 172, 338, 394
BstX2I RGATCY 2 cut(s) 283, 506
BstYI RGATCY 2 cut(s) 283, 506
BstZI CGGCCG 1 cut(s) 423
BsuRI GGCC 7 cut(s) 36, 42, 49, 73, 308, 425, 598
BtgI CCRYGG 2 cut(s) 400, 434
BtgZI GCGATG 1 cut(s) 425
BtsCI GGATG 2 cut(s) 500, 550
Cac8I GCNNGC 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 8 cut(s) 4, 35, 41, 114, 396, 488, 564, 597
CpoI CGGWCCG 1 cut(s) 114
CseI GACGC 2 cut(s) 306, 538
Csp6I GTAC 1 cut(s) 538
CspI CGGWCCG 1 cut(s) 114
CviAII CATG 5 cut(s) 26, 175, 401, 435, 532
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 1 cut(s) 185
DpnI GATC 6 cut(s) 204, 260, 285, 383, 508, 530
DpnII GATC 6 cut(s) 202, 258, 283, 381, 506, 528
DraIII CACNNNGTG 1 cut(s) 360
EaeI YGGCCR 2 cut(s) 71, 423
EagI CGGCCG 1 cut(s) 423
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 2 cut(s) 75, 88
EclXI CGGCCG 1 cut(s) 423
Eco130I CCWWGG 2 cut(s) 400, 434
Eco147I AGGCCT 1 cut(s) 49
Eco47I GGWCC 5 cut(s) 4, 114, 396, 488, 564
Eco52I CGGCCG 1 cut(s) 423
Eco88I CYCGRG 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 37, 130
EcoT14I CCWWGG 2 cut(s) 400, 434
ErhI CCWWGG 2 cut(s) 400, 434
FaeI CATG 5 cut(s) 29, 178, 404, 438, 535
FaiI YATR 8 cut(s) 27, 176, 248, 255, 402, 436, 533, 559
FaqI GGGAC 1 cut(s) 134
FatI CATG 5 cut(s) 25, 174, 400, 434, 531
FauI CCCGC 1 cut(s) 450
FokI GGATG 2 cut(s) 487, 557
FspBI CTAG 1 cut(s) 32
HaeIII GGCC 7 cut(s) 36, 42, 49, 73, 308, 425, 598
HapII CCGG 7 cut(s) 112, 117, 179, 358, 491, 504, 510
HgaI GACGC 2 cut(s) 306, 538
Hin1I GRCGYC 2 cut(s) 123, 549
Hin1II CATG 5 cut(s) 29, 178, 404, 438, 535
HinfI GANTC 1 cut(s) 494
HpaII CCGG 7 cut(s) 112, 117, 179, 358, 491, 504, 510
HphI GGTGA 3 cut(s) 253, 455, 489
Hpy166II GTNNAC 1 cut(s) 396
Hpy188I TCNGA 3 cut(s) 304, 568, 608
Hpy188III TCNNGA 2 cut(s) 491, 504
Hpy8I GTNNAC 1 cut(s) 396
Hpy99I CGWCG 2 cut(s) 300, 581
HpyAV CCTTC 2 cut(s) 105, 440
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 107, 360
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 2 cut(s) 123, 549
Hsp92II CATG 5 cut(s) 29, 178, 404, 438, 535
Kpn2I TCCGGA 2 cut(s) 490, 503
Kzo9I GATC 6 cut(s) 202, 258, 283, 381, 506, 528
LmnI GCTCC 1 cut(s) 454
Lsp1109I GCAGC 3 cut(s) 172, 338, 394
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 123
MalI GATC 6 cut(s) 204, 260, 285, 383, 508, 530
MbiI CCGCTC 2 cut(s) 457, 583
MboI GATC 6 cut(s) 202, 258, 283, 381, 506, 528
MboII GAAGA 1 cut(s) 22
MflI RGATCY 2 cut(s) 283, 506
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 2 cut(s) 216, 270
MmeI TCCRAC 2 cut(s) 151, 311
MroI TCCGGA 2 cut(s) 490, 503
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 2 cut(s) 173, 179
MspI CCGG 7 cut(s) 112, 117, 179, 358, 491, 504, 510
MspR9I CCNGG 6 cut(s) 39, 112, 118, 132, 358, 511
MvaI CCWGG 2 cut(s) 39, 132
MwoI GCNNNNNNNGC 3 cut(s) 75, 107, 360
NciI CCSGG 4 cut(s) 112, 118, 358, 511
NcoI CCATGG 2 cut(s) 400, 434
NdeII GATC 6 cut(s) 202, 258, 283, 381, 506, 528
NlaIII CATG 5 cut(s) 29, 178, 404, 438, 535
NlaIV GGNNCC 4 cut(s) 6, 398, 489, 508
NmeAIII GCCGAG 3 cut(s) 99, 334, 451
PceI AGGCCT 1 cut(s) 49
PfeI GAWTC 1 cut(s) 494
Psp6I CCWGG 2 cut(s) 37, 130
PspGI CCWGG 2 cut(s) 37, 130
PspN4I GGNNCC 4 cut(s) 6, 398, 489, 508
PspPI GGNCC 8 cut(s) 4, 35, 41, 114, 396, 488, 564, 597
PsuI RGATCY 2 cut(s) 283, 506
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 2 cut(s) 173, 179
Rsr2I CGGWCCG 1 cut(s) 114
RsrII CGGWCCG 1 cut(s) 114
SaqAI TTAA 1 cut(s) 17
Sau3AI GATC 6 cut(s) 202, 258, 283, 381, 506, 528
Sau96I GGNCC 8 cut(s) 4, 35, 41, 114, 396, 488, 564, 597
ScaI AGTACT 1 cut(s) 539
ScrFI CCNGG 6 cut(s) 39, 112, 118, 132, 358, 511
SduI GDGCHC 1 cut(s) 80
SetI ASST 3 cut(s) 71, 126, 445
SinI GGWCC 5 cut(s) 4, 114, 396, 488, 564
SmiMI CAYNNNNRTG 2 cut(s) 173, 179
Sse9I AATT 2 cut(s) 216, 270
SseBI AGGCCT 1 cut(s) 49
SspMI CTAG 1 cut(s) 32
StuI AGGCCT 1 cut(s) 49
StyD4I CCNGG 6 cut(s) 37, 110, 116, 130, 356, 509
StyI CCWWGG 2 cut(s) 400, 434
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 213, 295
TasI AATT 2 cut(s) 216, 270
TatI WGTACW 1 cut(s) 537
TauI GCSGC 8 cut(s) 91, 94, 104, 107, 110, 425, 576, 586
TfiI GAWTC 1 cut(s) 494
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseI GCWGC 3 cut(s) 160, 351, 407
VpaK11BI GGWCC 5 cut(s) 4, 114, 396, 488, 564
XapI RAATTY 1 cut(s) 216
XspI CTAG 1 cut(s) 32
ZraI GACGTC 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.