FvH4_2g10930

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001054
Physical Location & Seq
Reverse (-)
9572662 .. 9583070
10409 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10930.t1

Sequence Viewer

Length: 1611 bp
ATGTCTATGGCCAATCCTCCACATGAAGAATCAAAGTCAGTCGTCAACATTGCCTCAACCTCATTAAGAAGGAAACCAAAGCGTCCGTCCTGGTTCACCCCCAAGAGGTTACTTCTCATATTTTGTGTGATTAACTTGATAAATTATGTGGACCGAGGTGCAATATCAAGCAACGGTGTGAATGGGAACCTTGAAATTTGTGAGAATGGTATATGCACACCTCCTACCGGCATACAGGGGTATTTTTTATTGACAAATTTTAAAGTTGGATGTCTATCGTCAGCTTTTTTGGTTGGACTTTTGGTGGCTTCTCCAATTTTTGCATCACTAGCAAAGAGTGTAAATCCATTTAGGCTTATTGGAGTTGGATTGTCAGTATGGACTTTTGCTGTAACTGGTTGTGGTTTTTCATTCAGCTTCTTGTCTCTCTTCATCTGTCGCATGCTAGTTGGTGTTGGTGAGGCTTCATTTATTAGTCTTGCAGCTCCATTTATTGATGATAATGCTCCAGCTGCTCAGAAAACTATATGGCTTTCGACATTCTACATGTGCATACCATCTGGATATGCACTTGGTTATGTTTACGGAGGCTTGGTGGGAACTTCTCTCAGTTGGCGTGCTGCATTTTGGGGGGAGGCAATTTTGATGCTTCCATTTGCTATTCTAGGATTTGTTATGAAGCCATTGCGGATGAAAGGGTTTAAGCACAATGAATCAACTGAAGCAATGATTTTGCATGAGACGGTTGTCTCAGAAGTTCAAGGTGCTTTGAACCCCAATGATAGGGCAATTTCTATGAAGGAAGATGTAACAAATTCTATCACACATGAACTTGCCAAGCCAAAATTTGCAACAAAATTAAAGAATCATCTTTCAAGGTTTATGAATGATATGAAGGATCTTTTGGTTGACAAGGTTTATATCATTGATGTTCTTGGTTATGTAGCATACACCTTTGTAATCGGTTCGTACTCATATTACGGGCCTAAGGCCGGCTATAATATATATCATATGACTAATGCAGATATGATTTTTGGAGGTATTACAATTGTGTGCGGAATATTAGGTACACTAGCGGGAGGCTTCCTTCTGGACTTCATGTCTAACACTATCTCCAATGCTTTCAAGCTTCTTTCAACAGCAACTTTTCTTGGGGCATTATTATGCTTTGGTGCCTTTTGCTGCAAGAACATGTATGGTTTCCTTGCTCTTTTTGCAGTTGGGCAACTACTTCTCTTTGCAACTATGGGTCCTGTAAATTTCGTTTGTCTCCATTGTGTTAAACCTAGTTTGAGGCCACTATCTATGGCCATTTCGACTGTTGCAATTCACATATTTGGAGATGTGCCTTCCTCACCTGTTGTTGGAGTTGTCCAGGACCACATGAAGAATTGGAGGTCAACTGTTCTTATTCTAACATCCATTTTAATTCCAGCAGCGGGCATATGGTTTACAGGAATTTTCTTGGACAGCGCGGATAGATCTGAAGAAGAAAGTGACGATGAAGAAAGTGATGATGTGATAACAACTGAAACACCGTCGCTTGAAGAGAAGAAGACAGAAACAACTGAATCTGCAGCTGAACCTGTCACATATTCAACTGGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

537

Amino Acids

58.28

Weight (kDa)

6.16

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 77 - 444 8.7e-28 Major Facilitator Superfamily
OATP PF03137 140 - 397 3e-20 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 394 - 461 3.5e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1172
AccII CGCG 1 cut(s) 1475
AciI CCGC 5 cut(s) 688, 1056, 1076, 1439, 1475
AclWI GGATC 1 cut(s) 906
AcoI YGGCCR 2 cut(s) 9, 1308
AcsI RAATTY 6 cut(s) 195, 256, 814, 845, 1258, 1458
AcuI CTGAAG 2 cut(s) 741, 1506
AfaI GTAC 2 cut(s) 971, 1069
AfiI CCNNNNNNNGG 6 cut(s) 105, 227, 783, 992, 1364, 1439
AflIII ACRYGT 2 cut(s) 546, 1191
AgsI TTSAA 8 cut(s) 194, 761, 772, 876, 1126, 1137, 1547, 1599
AhdI GACNNNNNGTC 1 cut(s) 1099
AjnI CCWGG 2 cut(s) 89, 1374
AjuI GAANNNNNNNTTGG 2 cut(s) 887, 919
AluBI AGCT 6 cut(s) 284, 417, 485, 512, 1129, 1580
AluI AGCT 6 cut(s) 284, 417, 485, 512, 1129, 1580
Alw26I GTCTC 4 cut(s) 429, 734, 754, 1274
AlwI GGATC 1 cut(s) 906
AoxI GGCC 5 cut(s) 9, 983, 990, 1295, 1308
ApeKI GCWGC 6 cut(s) 482, 512, 620, 1182, 1436, 1577
ApoI RAATTY 6 cut(s) 195, 256, 814, 845, 1258, 1458
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
AspLEI GCGC 1 cut(s) 1475
AspS9I GGNCC 4 cut(s) 151, 983, 1250, 1378
AsuHPI GGTGA 3 cut(s) 88, 470, 1347
AvaII GGWCC 3 cut(s) 151, 1250, 1378
AxyI CCTNAGG 1 cut(s) 987
BalI TGGCCA 2 cut(s) 11, 1310
BanI GGYRCC 1 cut(s) 1172
BbsI GAAGAC 1 cut(s) 1562
BbvI GCAGC 6 cut(s) 494, 499, 607, 1169, 1448, 1589
BccI CCATC 1 cut(s) 565
BciT130I CCWGG 2 cut(s) 91, 1376
BcoDI GTCTC 4 cut(s) 429, 734, 754, 1274
BfaI CTAG 5 cut(s) 329, 446, 665, 1073, 1287
BfmI CTRYAG 1 cut(s) 1575
BglII AGATCT 1 cut(s) 1481
BisI GCNGC 6 cut(s) 483, 513, 621, 1183, 1437, 1578
BlsI GCNGC 6 cut(s) 484, 514, 622, 1184, 1438, 1579
Bme1390I CCNGG 2 cut(s) 91, 1376
Bme18I GGWCC 3 cut(s) 151, 1250, 1378
BmeRI GACNNNNNGTC 1 cut(s) 1099
BmgT120I GGNCC 4 cut(s) 151, 983, 1250, 1378
BmiI GGNNCC 3 cut(s) 188, 1174, 1251
BmrFI CCNGG 2 cut(s) 91, 1376
BmsI GCATC 2 cut(s) 332, 636
BoxI GACNNNNGTC 1 cut(s) 746
BpiI GAAGAC 1 cut(s) 1562
BpmI CTGGAG 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 274
BsaJI CCNNGG 1 cut(s) 154
BsaXI ACNNNNNCTCC 4 cut(s) 579, 609, 1097, 1127
Bsc4I CCNNNNNNNGG 6 cut(s) 105, 227, 783, 992, 1364, 1439
Bse118I RCCGGY 2 cut(s) 227, 992
Bse1I ACTGG 2 cut(s) 400, 1606
Bse21I CCTNAGG 1 cut(s) 987
Bse3DI GCAATG 3 cut(s) 48, 683, 732
Bse8I GATNNNNATC 1 cut(s) 274
BseBI CCWGG 2 cut(s) 91, 1376
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 3 cut(s) 275, 696, 1418
BseJI GATNNNNATC 1 cut(s) 274
BseLI CCNNNNNNNGG 6 cut(s) 105, 227, 783, 992, 1364, 1439
BseMI GCAATG 3 cut(s) 48, 683, 732
BseMII CTCAG 3 cut(s) 530, 622, 765
BseNI ACTGG 2 cut(s) 400, 1606
BseXI GCAGC 6 cut(s) 494, 499, 607, 1169, 1448, 1589
Bsh1236I CGCG 1 cut(s) 1475
BshFI GGCC 5 cut(s) 11, 985, 992, 1297, 1310
BshNI GGYRCC 1 cut(s) 1172
BsiSI CCGG 2 cut(s) 228, 993
BslI CCNNNNNNNGG 6 cut(s) 105, 227, 783, 992, 1364, 1439
BsmAI GTCTC 4 cut(s) 429, 734, 754, 1274
BsmBI CGTCTC 1 cut(s) 734
BsnI GGCC 5 cut(s) 11, 985, 992, 1297, 1310
Bsp143I GATC 2 cut(s) 898, 1481
BspACI CCGC 5 cut(s) 688, 1056, 1076, 1439, 1475
BspANI GGCC 5 cut(s) 11, 985, 992, 1297, 1310
BspCNI CTCAG 3 cut(s) 529, 621, 764
BspFNI CGCG 1 cut(s) 1475
BspLI GGNNCC 3 cut(s) 188, 1174, 1251
BspMAI CTGCAG 1 cut(s) 1579
BspPI GGATC 1 cut(s) 906
BspT107I GGYRCC 1 cut(s) 1172
BsrDI GCAATG 3 cut(s) 48, 683, 732
BsrFI RCCGGY 2 cut(s) 227, 992
BsrI ACTGG 2 cut(s) 400, 1606
BssAI RCCGGY 2 cut(s) 227, 992
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 2 cut(s) 898, 1481
Bst2UI CCWGG 2 cut(s) 91, 1376
Bst4CI ACNGT 5 cut(s) 176, 745, 1321, 1405, 1539
Bst6I CTCTTC 2 cut(s) 434, 1542
BstC8I GCNNGC 4 cut(s) 443, 618, 994, 1441
BstDEI CTNAG 4 cut(s) 516, 608, 751, 987
BstF5I GGATG 3 cut(s) 275, 696, 1418
BstFNI CGCG 1 cut(s) 1475
BstHHI GCGC 1 cut(s) 1475
BstKTI GATC 2 cut(s) 901, 1484
BstMAI GTCTC 4 cut(s) 429, 734, 754, 1274
BstMBI GATC 2 cut(s) 898, 1481
BstMWI GCNNNNNNNGC 3 cut(s) 329, 512, 1214
BstNI CCWGG 2 cut(s) 91, 1376
BstNSI RCATGY 3 cut(s) 445, 550, 1195
BstPAI GACNNNNGTC 1 cut(s) 746
BstSCI CCNGG 2 cut(s) 89, 1374
BstSFI CTRYAG 1 cut(s) 1575
BstUI CGCG 1 cut(s) 1475
BstV1I GCAGC 6 cut(s) 494, 499, 607, 1169, 1448, 1589
BstV2I GAAGAC 1 cut(s) 1562
BstX2I RGATCY 2 cut(s) 898, 1481
BstYI RGATCY 2 cut(s) 898, 1481
Bsu36I CCTNAGG 1 cut(s) 987
BsuRI GGCC 5 cut(s) 11, 985, 992, 1297, 1310
BtsCI GGATG 3 cut(s) 275, 696, 1418
Cac8I GCNNGC 4 cut(s) 443, 618, 994, 1441
CfoI GCGC 1 cut(s) 1475
Cfr10I RCCGGY 2 cut(s) 227, 992
Cfr13I GGNCC 4 cut(s) 151, 983, 1250, 1378
CseI GACGC 1 cut(s) 71
Csp6I GTAC 2 cut(s) 970, 1068
CviAII CATG 8 cut(s) 23, 442, 547, 737, 827, 1099, 1192, 1384
CviQI GTAC 2 cut(s) 970, 1068
DdeI CTNAG 4 cut(s) 516, 608, 751, 987
DpnI GATC 2 cut(s) 900, 1483
DpnII GATC 2 cut(s) 898, 1481
DraI TTTAAA 1 cut(s) 262
DriI GACNNNNNGTC 1 cut(s) 1099
EaeI YGGCCR 2 cut(s) 9, 1308
Eam1104I CTCTTC 2 cut(s) 434, 1542
Eam1105I GACNNNNNGTC 1 cut(s) 1099
EarI CTCTTC 2 cut(s) 434, 1542
Eco47I GGWCC 3 cut(s) 151, 1250, 1378
Eco57I CTGAAG 2 cut(s) 741, 1506
Eco81I CCTNAGG 1 cut(s) 987
EcoO109I RGGNCCY 1 cut(s) 1250
EcoRII CCWGG 2 cut(s) 89, 1374
Esp3I CGTCTC 1 cut(s) 734
FaeI CATG 8 cut(s) 26, 445, 550, 740, 830, 1102, 1195, 1387
FatI CATG 8 cut(s) 22, 441, 546, 736, 826, 1098, 1191, 1383
FauI CCCGC 2 cut(s) 1069, 1432
FauNDI CATATG 2 cut(s) 1011, 1445
Fnu4HI GCNGC 6 cut(s) 483, 513, 621, 1183, 1437, 1578
FokI GGATG 3 cut(s) 282, 703, 1405
Fsp4HI GCNGC 6 cut(s) 483, 513, 621, 1183, 1437, 1578
FspBI CTAG 5 cut(s) 329, 446, 665, 1073, 1287
GlaI GCGC 1 cut(s) 1474
GluI GCNGC 6 cut(s) 483, 513, 621, 1183, 1437, 1578
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 5 cut(s) 11, 985, 992, 1297, 1310
HapII CCGG 2 cut(s) 228, 993
HgaI GACGC 1 cut(s) 71
HhaI GCGC 1 cut(s) 1475
Hin1II CATG 8 cut(s) 26, 445, 550, 740, 830, 1102, 1195, 1387
Hin6I GCGC 1 cut(s) 1473
HinP1I GCGC 1 cut(s) 1473
HincII GTYRAC 3 cut(s) 46, 910, 1401
HindII GTYRAC 3 cut(s) 46, 910, 1401
HindIII AAGCTT 1 cut(s) 1127
HinfI GANTC 4 cut(s) 29, 713, 865, 1571
HpaII CCGG 2 cut(s) 228, 993
HphI GGTGA 3 cut(s) 88, 470, 1347
Hpy166II GTNNAC 8 cut(s) 46, 96, 151, 583, 910, 1070, 1401, 1452
Hpy188I TCNGA 3 cut(s) 519, 754, 1486
Hpy188III TCNNGA 2 cut(s) 561, 1091
Hpy8I GTNNAC 8 cut(s) 46, 96, 151, 583, 910, 1070, 1401, 1452
Hpy99I CGWCG 1 cut(s) 1543
HpyAV CCTTC 5 cut(s) 63, 793, 889, 1097, 1359
HpyCH4III ACNGT 5 cut(s) 176, 745, 1321, 1405, 1539
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 512, 1214
HpyF3I CTNAG 4 cut(s) 516, 608, 751, 987
Hsp92II CATG 8 cut(s) 26, 445, 550, 740, 830, 1102, 1195, 1387
HspAI GCGC 1 cut(s) 1473
KroI GCCGGC 1 cut(s) 992
KroNI GCCGGC 1 cut(s) 994
Kzo9I GATC 2 cut(s) 898, 1481
LmnI GCTCC 2 cut(s) 490, 511
Lsp1109I GCAGC 6 cut(s) 494, 499, 607, 1169, 1448, 1589
LweI GCATC 2 cut(s) 332, 636
MaeI CTAG 5 cut(s) 329, 446, 665, 1073, 1287
MaeIII GTNAC 5 cut(s) 108, 391, 808, 1496, 1588
MalI GATC 2 cut(s) 900, 1483
MboI GATC 2 cut(s) 898, 1481
MfeI CAATTG 1 cut(s) 1047
MflI RGATCY 2 cut(s) 898, 1481
MlsI TGGCCA 2 cut(s) 11, 1310
MluNI TGGCCA 2 cut(s) 11, 1310
MmeI TCCRAC 4 cut(s) 247, 274, 346, 1345
Mox20I TGGCCA 2 cut(s) 11, 1310
MroNI GCCGGC 1 cut(s) 992
MscI TGGCCA 2 cut(s) 11, 1310
MseI TTAA 7 cut(s) 65, 132, 261, 702, 860, 1281, 1427
MslI CAYNNNNRTG 1 cut(s) 1162
Msp20I TGGCCA 2 cut(s) 11, 1310
MspA1I CMGCKG 3 cut(s) 512, 1439, 1580
MspI CCGG 2 cut(s) 228, 993
MspR9I CCNGG 2 cut(s) 91, 1376
MunI CAATTG 1 cut(s) 1047
MvaI CCWGG 2 cut(s) 91, 1376
MvnI CGCG 1 cut(s) 1475
MwoI GCNNNNNNNGC 3 cut(s) 329, 512, 1214
NaeI GCCGGC 1 cut(s) 994
NdeI CATATG 2 cut(s) 1011, 1445
NdeII GATC 2 cut(s) 898, 1481
NgoMIV GCCGGC 1 cut(s) 992
NlaIII CATG 8 cut(s) 26, 445, 550, 740, 830, 1102, 1195, 1387
NlaIV GGNNCC 3 cut(s) 188, 1174, 1251
NmuCI GTSAC 2 cut(s) 1496, 1588
NspI RCATGY 3 cut(s) 445, 550, 1195
PaeI GCATGC 1 cut(s) 445
PciI ACATGT 2 cut(s) 546, 1191
PdiI GCCGGC 1 cut(s) 994
PfeI GAWTC 4 cut(s) 29, 713, 865, 1571
PfoI TCCNGGA 1 cut(s) 1374
PkrI GCNGC 6 cut(s) 484, 514, 622, 1184, 1438, 1579
PpuMI RGGWCCY 1 cut(s) 1250
PscI ACATGT 2 cut(s) 546, 1191
PshAI GACNNNNGTC 1 cut(s) 746
Psp5II RGGWCCY 1 cut(s) 1250
Psp6I CCWGG 2 cut(s) 89, 1374
PspGI CCWGG 2 cut(s) 89, 1374
PspN4I GGNNCC 3 cut(s) 188, 1174, 1251
PspPI GGNCC 4 cut(s) 151, 983, 1250, 1378
PspPPI RGGWCCY 1 cut(s) 1250
PstI CTGCAG 1 cut(s) 1579
PsuI RGATCY 2 cut(s) 898, 1481
PvuII CAGCTG 2 cut(s) 512, 1580
RsaI GTAC 2 cut(s) 971, 1069
RsaNI GTAC 2 cut(s) 970, 1068
RseI CAYNNNNRTG 1 cut(s) 1162
SaqAI TTAA 7 cut(s) 65, 132, 261, 702, 860, 1281, 1427
SatI GCNGC 6 cut(s) 483, 513, 621, 1183, 1437, 1578
Sau3AI GATC 2 cut(s) 898, 1481
Sau96I GGNCC 4 cut(s) 151, 983, 1250, 1378
ScrFI CCNGG 2 cut(s) 91, 1376
SfaNI GCATC 2 cut(s) 332, 636
SfcI CTRYAG 1 cut(s) 1575
SinI GGWCC 3 cut(s) 151, 1250, 1378
SmiMI CAYNNNNRTG 1 cut(s) 1162
SphI GCATGC 1 cut(s) 445
SsiI CCGC 5 cut(s) 688, 1056, 1076, 1439, 1475
SspI AATATT 1 cut(s) 1062
SspMI CTAG 5 cut(s) 329, 446, 665, 1073, 1287
StyD4I CCNGG 2 cut(s) 89, 1374
TaaI ACNGT 5 cut(s) 176, 745, 1321, 1405, 1539
TaqI TCGA 2 cut(s) 536, 1316
TaqII GACCGA 1 cut(s) 168
TfiI GAWTC 4 cut(s) 29, 713, 865, 1571
Tru1I TTAA 7 cut(s) 65, 132, 261, 702, 860, 1281, 1427
Tru9I TTAA 7 cut(s) 65, 132, 261, 702, 860, 1281, 1427
TseFI GTSAC 2 cut(s) 1496, 1588
TseI GCWGC 6 cut(s) 482, 512, 620, 1182, 1436, 1577
Tsp45I GTSAC 2 cut(s) 1496, 1588
TspGWI ACGGA 2 cut(s) 75, 600
VpaK11BI GGWCC 3 cut(s) 151, 1250, 1378
XapI RAATTY 6 cut(s) 195, 256, 814, 845, 1258, 1458
XceI RCATGY 3 cut(s) 445, 550, 1195
XspI CTAG 5 cut(s) 329, 446, 665, 1073, 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.