Rw6G014240

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001054
Physical Location & Seq
Forward (+)
26356926 .. 26367040
10115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014240.1

Sequence Viewer

Length: 1425 bp
ATGTTACATCAATGCCAATTTTTTTCGCCATTGAGCTGGAATAATCCCCTAGTGACGCGTCTGATCACCGCCTGGATATCGATCAAGAACTTGGTGGTGCATAGCACCTCGTTTTCTGGTTCAGAAACGACGTCCACAAAATCTGGGTTGACATATACGTCACAGAGAGCAAATGGAATTAAACTTCGGAACCTGTTCCCATTACAACCATTTACCGAAGTTGACTCCGCACACCGGCTCCATCTGTCAATCTTGTCACCAGTGCACATCAACATTATAAATATTTCCATCTCATCACATTTATTTCTTTGGAAAATTACATCAGAGTTACCAGGCTTGGGGATAGAGAAAGAGGGACGGGTTTCCAATTTTGGAAATCTGTCCTTCTTTACAATTTTTGCACAATTTTTACATGCAATTCTTCCAATGGGTGCGGAGACGCCATGGGACCATGCTAGGATGACTGAAATAATGCAAAACCAACATGGAATCTGTCTCAATCCACAAAGAAAACCAAAAGAAAAAATGGGGAGTACTGACGAGGTTGATAGGGCAGTTTCAATGAAGGAAGATGTAACAAATTCTACCATACATAAACCTTCCAAGCCAAAAATTGCAACAAAAATAAAGAATCATCTTTCAAGGTTCATGAAAGATATGAAGGATCTTTTGGTCGATAAGGTTTATGTTGTTAATGTTCTTGGTTATGTAGCATACAACTTTGTACTTGGTTCGTACTCATATTGGGGGCCTAAGGCTGGTTATAATATCTATCATATGACTAATGCAGATATGATTTTTGGAGGTATTACAATTGTGTGCGGAATATTGGGAACACTAGCAGGAGGCTTTCTTCTGGATTTCATGTCTAACACTATGTCCAATGGTTTCAAGCTTCTTTCAACAGCAACGCTTTTTGGGGCAATATTATGCTTTGGTGCCTTTTGTTTGAAGAACATGTATGGTTTCCTAGCTCTTTTGCAGTTGGGCAACTACTTCTCTTTGCAACTCAGGTACAAATCCAATCAAATAAGTGACCCACTTCTTATTTGGTGGGGTCCTGTAAATTTTGTTTGTCTCCATTGTGTTAAACCTAGTTTGAGGCCACTATCTATGGCCATTTCTACTGTTGCAATTCACATATTTGGAGATGTGCCTTCCTCACCTCTTGTTGGAGTTGTCCAGGATCACATCCAGAATTGGAGGTCAACTGCTCTTATTCTAACATCCGTTTTGATTCCTGCAGCGGCCATATGGTTTACAGCAATTTTCATGGACAGCGTGGATAGATATGACGAAGAAAGTGATGATGAAGAAAGTGATGATGAGATAGAAACAACTGAAATGCCGTCGCTTGAAGAGAAGAAGACAGAGAAAACAACTGAATCTGCTGCTCAACCTGTCACATATTCAACTGTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

474

Amino Acids

52.94

Weight (kDa)

6.57

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OATP PF03137 172 - 316 2.5e-12 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 278, 765
AasI GACNNNNNNGTC 1 cut(s) 157
AatII GACGTC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 938
AccII CGCG 1 cut(s) 58
AciI CCGC 5 cut(s) 69, 228, 434, 822, 1247
AclWI GGATC 2 cut(s) 672, 1194
AcoI YGGCCR 2 cut(s) 1116, 1248
AcsI RAATTY 2 cut(s) 580, 1066
AcyI GRCGYC 2 cut(s) 131, 440
AfaI GTAC 4 cut(s) 535, 726, 737, 1016
AfiI CCNNNNNNNGG 4 cut(s) 234, 338, 758, 1172
AflIII ACRYGT 2 cut(s) 56, 957
AgsI TTSAA 7 cut(s) 561, 642, 892, 903, 952, 1358, 1413
AjnI CCWGG 3 cut(s) 71, 331, 1182
AjuI GAANNNNNNNTTGG 2 cut(s) 653, 685
AluBI AGCT 3 cut(s) 36, 895, 974
AluI AGCT 3 cut(s) 36, 895, 974
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 3 cut(s) 431, 500, 1082
Alw44I GTGCAC 1 cut(s) 263
AlwI GGATC 2 cut(s) 672, 1194
AoxI GGCC 4 cut(s) 749, 1103, 1116, 1248
ApaLI GTGCAC 1 cut(s) 263
ApeKI GCWGC 2 cut(s) 1244, 1391
ApoI RAATTY 2 cut(s) 580, 1066
Asp700I GAANNNNTTC 1 cut(s) 194
AspS9I GGNCC 3 cut(s) 448, 749, 1058
AsuHPI GGTGA 3 cut(s) 58, 249, 1155
AvaII GGWCC 2 cut(s) 448, 1058
AxyI CCTNAGG 1 cut(s) 753
BaeGI GKGCMC 1 cut(s) 267
BalI TGGCCA 1 cut(s) 1118
BanI GGYRCC 1 cut(s) 938
BbsI GAAGAC 1 cut(s) 1373
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 1256, 1378
BccI CCATC 2 cut(s) 249, 296
BceAI ACGGC 1 cut(s) 1333
BciT130I CCWGG 3 cut(s) 73, 333, 1184
BclI TGATCA 1 cut(s) 63
BcoDI GTCTC 3 cut(s) 431, 500, 1082
BfaI CTAG 6 cut(s) 50, 456, 839, 971, 1095, 1423
BfmI CTRYAG 1 cut(s) 1242
BisI GCNGC 3 cut(s) 1245, 1248, 1392
BlsI GCNGC 3 cut(s) 1246, 1249, 1393
BmcAI AGTACT 1 cut(s) 535
Bme1390I CCNGG 3 cut(s) 73, 333, 1184
Bme18I GGWCC 2 cut(s) 448, 1058
BmgT120I GGNCC 3 cut(s) 448, 749, 1058
BmiI GGNNCC 6 cut(s) 191, 239, 449, 750, 940, 1059
BmrFI CCNGG 3 cut(s) 73, 333, 1184
BpiI GAAGAC 1 cut(s) 1373
Bsa29I ATCGAT 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 80
BsaHI GRCGYC 2 cut(s) 131, 440
BsaJI CCNNGG 1 cut(s) 443
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 338, 758, 1172
Bse118I RCCGGY 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 260
Bse21I CCTNAGG 1 cut(s) 753
Bse8I GATNNNNATC 1 cut(s) 80
BseBI CCWGG 3 cut(s) 73, 333, 1184
BseCI ATCGAT 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 443
BseGI GGATG 3 cut(s) 465, 1191, 1226
BseJI GATNNNNATC 1 cut(s) 80
BseLI CCNNNNNNNGG 4 cut(s) 234, 338, 758, 1172
BseMII CTCAG 1 cut(s) 1024
BseNI ACTGG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 2 cut(s) 1256, 1378
Bsh1236I CGCG 1 cut(s) 58
BshFI GGCC 4 cut(s) 751, 1105, 1118, 1250
BshNI GGYRCC 1 cut(s) 938
BshVI ATCGAT 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 1 cut(s) 235
BslFI GGGAC 2 cut(s) 369, 461
BslI CCNNNNNNNGG 4 cut(s) 234, 338, 758, 1172
BsmAI GTCTC 3 cut(s) 431, 500, 1082
BsmBI CGTCTC 1 cut(s) 431
BsmFI GGGAC 2 cut(s) 369, 461
BsnI GGCC 4 cut(s) 751, 1105, 1118, 1250
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 4 cut(s) 63, 81, 664, 1186
Bsp19I CCATGG 1 cut(s) 443
BspACI CCGC 5 cut(s) 69, 228, 434, 822, 1247
BspANI GGCC 4 cut(s) 751, 1105, 1118, 1250
BspCNI CTCAG 1 cut(s) 1023
BspDI ATCGAT 1 cut(s) 80
BspFNI CGCG 1 cut(s) 58
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 6 cut(s) 191, 239, 449, 750, 940, 1059
BspMAI CTGCAG 1 cut(s) 1246
BspPI GGATC 2 cut(s) 672, 1194
BspT107I GGYRCC 1 cut(s) 938
BsrFI RCCGGY 1 cut(s) 234
BsrI ACTGG 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 443
BssMI GATC 4 cut(s) 63, 81, 664, 1186
BssNI GRCGYC 2 cut(s) 131, 440
BssT1I CCWWGG 1 cut(s) 443
Bst2UI CCWGG 3 cut(s) 73, 333, 1184
Bst4CI ACNGT 2 cut(s) 1129, 1417
Bst6I CTCTTC 1 cut(s) 1353
BstACI GRCGYC 2 cut(s) 131, 440
BstDEI CTNAG 2 cut(s) 753, 1010
BstDSI CCRYGG 1 cut(s) 443
BstF5I GGATG 3 cut(s) 465, 1191, 1226
BstFNI CGCG 1 cut(s) 58
BstKTI GATC 4 cut(s) 66, 84, 667, 1189
BstMAI GTCTC 3 cut(s) 431, 500, 1082
BstMBI GATC 4 cut(s) 63, 81, 664, 1186
BstNI CCWGG 3 cut(s) 73, 333, 1184
BstNSI RCATGY 2 cut(s) 416, 961
BstSCI CCNGG 3 cut(s) 71, 331, 1182
BstSFI CTRYAG 1 cut(s) 1242
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 1256, 1378
BstV2I GAAGAC 1 cut(s) 1373
BstX2I RGATCY 1 cut(s) 664
BstXI CCANNNNNNTGG 1 cut(s) 36
BstYI RGATCY 1 cut(s) 664
Bsu15I ATCGAT 1 cut(s) 80
Bsu36I CCTNAGG 1 cut(s) 753
BsuRI GGCC 4 cut(s) 751, 1105, 1118, 1250
BsuTUI ATCGAT 1 cut(s) 80
BtgI CCRYGG 1 cut(s) 443
BtsCI GGATG 3 cut(s) 465, 1191, 1226
BtsIMutI CAGTG 1 cut(s) 267
CciI TCATGA 1 cut(s) 648
Cfr10I RCCGGY 1 cut(s) 234
Cfr13I GGNCC 3 cut(s) 448, 749, 1058
ClaI ATCGAT 1 cut(s) 80
CseI GACGC 3 cut(s) 47, 64, 448
Csp6I GTAC 4 cut(s) 534, 725, 736, 1015
CviAII CATG 8 cut(s) 413, 444, 452, 485, 649, 865, 958, 1273
CviQI GTAC 4 cut(s) 534, 725, 736, 1015
DdeI CTNAG 2 cut(s) 753, 1010
DpnI GATC 4 cut(s) 65, 83, 666, 1188
DpnII GATC 4 cut(s) 63, 81, 664, 1186
DrdI GACNNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 2 cut(s) 1116, 1248
Eam1104I CTCTTC 1 cut(s) 1353
EarI CTCTTC 1 cut(s) 1353
Eco130I CCWWGG 1 cut(s) 443
Eco32I GATATC 1 cut(s) 78
Eco47I GGWCC 2 cut(s) 448, 1058
Eco81I CCTNAGG 1 cut(s) 753
EcoO109I RGGNCCY 2 cut(s) 749, 1058
EcoRII CCWGG 3 cut(s) 71, 331, 1182
EcoRV GATATC 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 443
Esp3I CGTCTC 1 cut(s) 431
FaeI CATG 8 cut(s) 416, 447, 455, 488, 652, 868, 961, 1276
FaqI GGGAC 2 cut(s) 369, 461
FatI CATG 8 cut(s) 412, 443, 451, 484, 648, 864, 957, 1272
FauNDI CATATG 2 cut(s) 777, 1253
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 3 cut(s) 1245, 1248, 1392
FokI GGATG 3 cut(s) 472, 1178, 1213
Fsp4HI GCNGC 3 cut(s) 1245, 1248, 1392
FspBI CTAG 6 cut(s) 50, 456, 839, 971, 1095, 1423
GluI GCNGC 3 cut(s) 1245, 1248, 1392
HaeIII GGCC 4 cut(s) 751, 1105, 1118, 1250
HapII CCGG 1 cut(s) 235
HgaI GACGC 3 cut(s) 47, 64, 448
Hin1I GRCGYC 2 cut(s) 131, 440
Hin1II CATG 8 cut(s) 416, 447, 455, 488, 652, 868, 961, 1276
HincII GTYRAC 3 cut(s) 150, 223, 1209
HindII GTYRAC 3 cut(s) 150, 223, 1209
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 5 cut(s) 224, 489, 631, 1237, 1385
HpaII CCGG 1 cut(s) 235
HphI GGTGA 3 cut(s) 58, 249, 1155
Hpy166II GTNNAC 6 cut(s) 135, 150, 223, 265, 1209, 1260
Hpy188I TCNGA 4 cut(s) 63, 124, 189, 325
Hpy188III TCNNGA 4 cut(s) 85, 649, 857, 1195
Hpy8I GTNNAC 6 cut(s) 135, 150, 223, 265, 1209, 1260
Hpy99I CGWCG 2 cut(s) 133, 1354
HpyAV CCTTC 5 cut(s) 394, 559, 609, 655, 1167
HpyCH4III ACNGT 2 cut(s) 1129, 1417
HpyCH4IV ACGT 2 cut(s) 131, 158
HpyF3I CTNAG 2 cut(s) 753, 1010
HpySE526I ACGT 2 cut(s) 131, 158
Hsp92I GRCGYC 2 cut(s) 131, 440
Hsp92II CATG 8 cut(s) 416, 447, 455, 488, 652, 868, 961, 1276
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 4 cut(s) 63, 81, 664, 1186
LmnI GCTCC 1 cut(s) 243
Lsp1109I GCAGC 2 cut(s) 1256, 1378
MaeI CTAG 6 cut(s) 50, 456, 839, 971, 1095, 1423
MaeII ACGT 2 cut(s) 131, 158
MaeIII GTNAC 8 cut(s) 3, 52, 159, 255, 327, 574, 1034, 1402
MalI GATC 4 cut(s) 65, 83, 666, 1188
MboI GATC 4 cut(s) 63, 81, 664, 1186
MboII GAAGA 9 cut(s) 413, 581, 845, 964, 1310, 1325, 1370, 1375, 1378
MfeI CAATTG 1 cut(s) 813
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 1 cut(s) 267
MlsI TGGCCA 1 cut(s) 1118
MluI ACGCGT 1 cut(s) 56
MluNI TGGCCA 1 cut(s) 1118
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 1153
MnlI CCTC 9 cut(s) 118, 346, 535, 797, 839, 1095, 1171, 1176, 1197
Mox20I TGGCCA 1 cut(s) 1118
MroXI GAANNNNTTC 1 cut(s) 194
MscI TGGCCA 1 cut(s) 1118
MseI TTAA 3 cut(s) 180, 693, 1089
Msp20I TGGCCA 1 cut(s) 1118
MspA1I CMGCKG 1 cut(s) 1247
MspI CCGG 1 cut(s) 235
MspR9I CCNGG 3 cut(s) 73, 333, 1184
MunI CAATTG 1 cut(s) 813
MvaI CCWGG 3 cut(s) 73, 333, 1184
MvnI CGCG 1 cut(s) 58
NcoI CCATGG 1 cut(s) 443
NdeI CATATG 2 cut(s) 777, 1253
NdeII GATC 4 cut(s) 63, 81, 664, 1186
NlaIII CATG 8 cut(s) 416, 447, 455, 488, 652, 868, 961, 1276
NlaIV GGNNCC 6 cut(s) 191, 239, 449, 750, 940, 1059
NmuCI GTSAC 5 cut(s) 52, 159, 255, 1034, 1402
NspI RCATGY 2 cut(s) 416, 961
PagI TCATGA 1 cut(s) 648
PciI ACATGT 1 cut(s) 957
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 4 cut(s) 489, 631, 1237, 1385
PfoI TCCNGGA 1 cut(s) 1182
PkrI GCNGC 3 cut(s) 1246, 1249, 1393
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PpuMI RGGWCCY 1 cut(s) 1058
PscI ACATGT 1 cut(s) 957
PsiI TTATAA 2 cut(s) 278, 765
Psp5II RGGWCCY 1 cut(s) 1058
Psp6I CCWGG 3 cut(s) 71, 331, 1182
PspGI CCWGG 3 cut(s) 71, 331, 1182
PspN4I GGNNCC 6 cut(s) 191, 239, 449, 750, 940, 1059
PspPI GGNCC 3 cut(s) 448, 749, 1058
PspPPI RGGWCCY 1 cut(s) 1058
PstI CTGCAG 1 cut(s) 1246
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 4 cut(s) 535, 726, 737, 1016
RsaNI GTAC 4 cut(s) 534, 725, 736, 1015
SaqAI TTAA 3 cut(s) 180, 693, 1089
SatI GCNGC 3 cut(s) 1245, 1248, 1392
Sau3AI GATC 4 cut(s) 63, 81, 664, 1186
Sau96I GGNCC 3 cut(s) 448, 749, 1058
ScaI AGTACT 1 cut(s) 535
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 3 cut(s) 73, 333, 1184
SduI GDGCHC 1 cut(s) 267
SfcI CTRYAG 1 cut(s) 1242
SinI GGWCC 2 cut(s) 448, 1058
SsiI CCGC 5 cut(s) 69, 228, 434, 822, 1247
SspI AATATT 3 cut(s) 283, 828, 927
SspMI CTAG 6 cut(s) 50, 456, 839, 971, 1095, 1423
StyD4I CCNGG 3 cut(s) 71, 331, 1182
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 2 cut(s) 1129, 1417
TaiI ACGT 2 cut(s) 134, 161
TaqI TCGA 2 cut(s) 80, 675
TatI WGTACW 2 cut(s) 533, 724
TauI GCSGC 1 cut(s) 1250
TfiI GAWTC 4 cut(s) 489, 631, 1237, 1385
Tru1I TTAA 3 cut(s) 180, 693, 1089
Tru9I TTAA 3 cut(s) 180, 693, 1089
TscAI CASTG 1 cut(s) 267
TseFI GTSAC 5 cut(s) 52, 159, 255, 1034, 1402
TseI GCWGC 2 cut(s) 1244, 1391
Tsp45I GTSAC 5 cut(s) 52, 159, 255, 1034, 1402
TspDTI ATGAA 7 cut(s) 578, 637, 665, 674, 853, 1261, 1326
TspGWI ACGGA 1 cut(s) 1219
TspRI CASTG 1 cut(s) 267
VneI GTGCAC 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 448, 1058
XapI RAATTY 2 cut(s) 580, 1066
XceI RCATGY 2 cut(s) 416, 961
XcmI CCANNNNNNNNNTGG 1 cut(s) 1047
XmnI GAANNNNTTC 1 cut(s) 194
XspI CTAG 6 cut(s) 50, 456, 839, 971, 1095, 1423
ZraI GACGTC 1 cut(s) 132
ZrmI AGTACT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.