MD10G1058300.v1.1

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001054
Physical Location & Seq
Forward (+)
7878425 .. 7886409
7985 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1058300.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGGCACAAAAGGAAGAAGCAACAAAGTCTTCTGAAGAAGGCAAGCCCAAGCCCCGTCCGGTGGACACAGCCATGGCTATTGCTTCAACCGCAGCTCCCGCTCCTTCCTGGTTCACGCCCAAGAGGTTACTTGTTATCTTTTGCTTGATTAACTTGATAAACTATGTGGACCGAGGAGCAATAGCAAGCAATGGTGTCAATGGGAGCCTTGGAACTTGTACAGATAGTGATATATGCACGGCTGGTACCGGGATACAGGGGGAGTTTCACTTGACTAACTTTCAAGATGGAGTTCTATCATCAGCTTTTATGGTTGGACTGCTTATTGCTTCTCCTATATTTGCATCATTAGCACAGAGTGTAAGTCCATTTAGGCTCATTGGAGTTGGATTATCAGTTTGGACCTTTGCGATAGTTGGTTGTGCTTGTTCATTCGATTTCTGGTCCATTACTCTTTGCCGCATGCTAGTTGGTGTTGGGGAAGCTTCGTTTATTAGTCTTGCAGCTCCATTCATTGACGATAATGCCCCAGCTGCTCAGAAAACTGCATGGCTTGGTATGTTCTACATGTGCATACCAAGTGGATATGCACTTGGCTATGTTTATGGTGGGGTGGTTGGAAGTCACACCAAGTGGCGTTATGCATTTGTGGGGGAAGCAATCCTTATGCTTCCATTTGCTATTCTAGGTTTTGTTATGAAGCCTTTGCAGCTGAAAGGTTTTTTTCATCCTGAATCAAAAAAAGCACTGACAGCAGTGGAAACGGCTGTTGAAGAAGTTCAAGCTAGTGATACATATGATTCAGCTATTTCGAATGGTGAACATTCTTCCTGTTCAATGAAGGAACATGTGAGAAATTCTACCTTACAAAAATCTTCCAAGTCAAATTTGGTATCAAGCATAAAGAATCAAATTTCAAGGTTTTTGAAAGATGTGAGGGTGCTATTGGTGGATAAGGTTTATGTTGTGAATGTTCTAGGTTACATAGCATACAACTTTGTCATAGGTGCATACTCATATTGGGGGCCTAAGGCTGGATATAATATATATCATATGACTGATGCAGATCTTATATTTGGAGGTATTACAATTGTGTGCGGAATATTGGGCACACTAGCGGGAGGCTTTCTTCTGGATTATATGACTAACACTATCTCTAATGCTTTTAAGCTTCTTTCAGTGGTAACGTTAATTGGGGGTGCCTTTTGCTTTGGTGCCTTTTGCTTCAAGAATATGTATGCTTTCCTAGCTCTTTTCGCAATCGGTGAACTACTTGTCTTTGCAACTCAGGGTCCTGTAAATTACATTAATCTCCATTGTGTTAAACCTAATATGAGGCCACTATCTATGGCTATGTCAACGGTTTCAATTCATATCTTCGGTGATGTACCTTCCGCACCTCTTGTTGGAGTTCTCCAGGATGCAATTAACAACTGGAGGAAGACTGCTCTTATTCTAACAGCTATTTTGTTTCCATCAGCTGTAATATGGTTTATAGGAATCTTTCTGCACAGCGTGGATAGATTTAACGAGGAAAGTGAACATCAGGTGACCATCCAAACCGAAAGGTCAAACACAACGCCGTTGCTTGAAGGGAAGAAGATAGAAACAACTGAATCTGTTGCTGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

545

Amino Acids

58.87

Weight (kDa)

6.37

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 84 - 455 1.4e-29 Major Facilitator Superfamily
OATP PF03137 149 - 406 7.3e-20 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 407 - 475 2e-07 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 245
AccB1I GGYRCC 3 cut(s) 245, 1199, 1214
AccBSI CCGCTC 1 cut(s) 101
AciI CCGC 6 cut(s) 90, 99, 460, 1098, 1118, 1395
AclI AACGTT 1 cut(s) 1187
AcsI RAATTY 3 cut(s) 856, 886, 912
AcuI CTGAAG 1 cut(s) 54
AdeI CACNNNGTG 3 cut(s) 359, 633, 1516
AfaI GTAC 3 cut(s) 220, 247, 1389
AfiI CCNNNNNNNGG 3 cut(s) 61, 1034, 1406
AflIII ACRYGT 2 cut(s) 567, 847
AjnI CCWGG 2 cut(s) 107, 1416
AoxI GGCC 2 cut(s) 1025, 1337
ApeKI GCWGC 4 cut(s) 92, 503, 533, 709
ApoI RAATTY 3 cut(s) 856, 886, 912
AseI ATTAAT 1 cut(s) 1308
Asp700I GAANNNNTTC 1 cut(s) 777
Asp718I GGTACC 1 cut(s) 245
AspS9I GGNCC 5 cut(s) 169, 402, 444, 1025, 1292
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 4 cut(s) 830, 1277, 1394, 1561
AsuII TTCGAA 1 cut(s) 812
AvaII GGWCC 4 cut(s) 169, 402, 444, 1292
AxyI CCTNAGG 1 cut(s) 1029
BaeGI GKGCMC 1 cut(s) 1112
BanI GGYRCC 3 cut(s) 245, 1199, 1214
BbsI GAAGAC 2 cut(s) 21, 1448
BbvI GCAGC 4 cut(s) 104, 515, 520, 721
BccI CCATC 3 cut(s) 281, 1483, 1562
BceAI ACGGC 3 cut(s) 255, 780, 1567
BciT130I CCWGG 2 cut(s) 109, 1418
BciVI GTATCC 1 cut(s) 246
BcnI CCSGG 1 cut(s) 250
BfaI CTAG 6 cut(s) 467, 686, 786, 977, 1115, 1247
BfuI GTATCC 1 cut(s) 246
BglII AGATCT 1 cut(s) 1066
BisI GCNGC 5 cut(s) 93, 460, 504, 534, 710
BlsI GCNGC 5 cut(s) 94, 461, 505, 535, 711
Bme1390I CCNGG 3 cut(s) 109, 250, 1418
Bme18I GGWCC 4 cut(s) 169, 402, 444, 1292
BmgT120I GGNCC 5 cut(s) 169, 402, 444, 1025, 1292
BmiI GGNNCC 6 cut(s) 206, 247, 1026, 1201, 1216, 1293
BmrFI CCNGG 3 cut(s) 109, 250, 1418
BmsI GCATC 3 cut(s) 353, 1051, 1411
BpiI GAAGAC 2 cut(s) 21, 1448
BpmI CTGGAG 2 cut(s) 1400, 1456
Bpu14I TTCGAA 1 cut(s) 812
BpuMI CCSGG 1 cut(s) 250
BsaBI GATNNNNATC 1 cut(s) 1065
BsaJI CCNNGG 3 cut(s) 72, 172, 208
BsaWI WCCGGW 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 1034, 1406
Bse1I ACTGG 1 cut(s) 1439
Bse21I CCTNAGG 1 cut(s) 1029
Bse3DI GCAATG 1 cut(s) 196
Bse8I GATNNNNATC 1 cut(s) 1065
BseBI CCWGG 2 cut(s) 109, 1418
BseDI CCNNGG 3 cut(s) 72, 172, 208
BseGI GGATG 3 cut(s) 727, 1426, 1554
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 3 cut(s) 61, 1034, 1406
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 2 cut(s) 551, 1301
BseNI ACTGG 1 cut(s) 1439
BseRI GAGGAG 1 cut(s) 189
BseSI GKGCMC 1 cut(s) 1112
BseXI GCAGC 4 cut(s) 104, 515, 520, 721
BseYI CCCAGC 1 cut(s) 529
BsgI GTGCAG 1 cut(s) 1493
BshFI GGCC 2 cut(s) 1027, 1339
BshNI GGYRCC 3 cut(s) 245, 1199, 1214
BsiSI CCGG 2 cut(s) 59, 249
BslI CCNNNNNNNGG 3 cut(s) 61, 1034, 1406
BsnI GGCC 2 cut(s) 1027, 1339
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 1 cut(s) 1112
Bsp1407I TGTACA 1 cut(s) 218
Bsp143I GATC 1 cut(s) 1066
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 6 cut(s) 90, 99, 460, 1098, 1118, 1395
BspANI GGCC 2 cut(s) 1027, 1339
BspCNI CTCAG 2 cut(s) 550, 1300
BspLI GGNNCC 6 cut(s) 206, 247, 1026, 1201, 1216, 1293
BspT104I TTCGAA 1 cut(s) 812
BspT107I GGYRCC 3 cut(s) 245, 1199, 1214
BsrBI CCGCTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 196
BsrGI TGTACA 1 cut(s) 218
BsrI ACTGG 1 cut(s) 1439
BssECI CCNNGG 3 cut(s) 72, 172, 208
BssMI GATC 1 cut(s) 1066
BssT1I CCWWGG 2 cut(s) 72, 208
Bst2UI CCWGG 2 cut(s) 109, 1418
Bst4CI ACNGT 1 cut(s) 1363
BstAUI TGTACA 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 3 cut(s) 44, 187, 464
BstDEI CTNAG 3 cut(s) 537, 1029, 1287
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 1549
BstF5I GGATG 3 cut(s) 727, 1426, 1554
BstKTI GATC 1 cut(s) 1069
BstMBI GATC 1 cut(s) 1066
BstMWI GCNNNNNNNGC 8 cut(s) 89, 98, 350, 533, 709, 752, 1247, 1256
BstNI CCWGG 2 cut(s) 109, 1418
BstNSI RCATGY 3 cut(s) 466, 571, 851
BstPI GGTNACC 1 cut(s) 1549
BstSCI CCNGG 3 cut(s) 107, 248, 1416
BstSLI GKGCMC 1 cut(s) 1112
BstV1I GCAGC 4 cut(s) 104, 515, 520, 721
BstV2I GAAGAC 2 cut(s) 21, 1448
BstX2I RGATCY 1 cut(s) 1066
BstYI RGATCY 1 cut(s) 1066
Bsu36I CCTNAGG 1 cut(s) 1029
BsuI GTATCC 1 cut(s) 246
BsuRI GGCC 2 cut(s) 1027, 1339
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 3 cut(s) 727, 1426, 1554
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 746, 762, 1185
Cac8I GCNNGC 3 cut(s) 44, 187, 464
Cfr13I GGNCC 5 cut(s) 169, 402, 444, 1025, 1292
Csp6I GTAC 3 cut(s) 219, 246, 1388
CviAII CATG 6 cut(s) 73, 463, 549, 568, 848, 1632
CviQI GTAC 3 cut(s) 219, 246, 1388
DdeI CTNAG 3 cut(s) 537, 1029, 1287
DpnI GATC 1 cut(s) 1068
DpnII GATC 1 cut(s) 1066
DraIII CACNNNGTG 3 cut(s) 359, 633, 1516
Eco130I CCWWGG 2 cut(s) 72, 208
Eco47I GGWCC 4 cut(s) 169, 402, 444, 1292
Eco57I CTGAAG 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 1029
Eco91I GGTNACC 1 cut(s) 1549
EcoO109I RGGNCCY 2 cut(s) 1025, 1292
EcoO65I GGTNACC 1 cut(s) 1549
EcoRII CCWGG 2 cut(s) 107, 1416
EcoT14I CCWWGG 2 cut(s) 72, 208
EcoT22I ATGCAT 1 cut(s) 646
ErhI CCWWGG 2 cut(s) 72, 208
FaeI CATG 6 cut(s) 76, 466, 552, 571, 851, 1635
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FatI CATG 6 cut(s) 72, 462, 548, 567, 847, 1631
FauI CCCGC 2 cut(s) 106, 1111
FauNDI CATATG 2 cut(s) 796, 1053
Fnu4HI GCNGC 5 cut(s) 93, 460, 504, 534, 710
FokI GGATG 3 cut(s) 714, 1433, 1541
Fsp4HI GCNGC 5 cut(s) 93, 460, 504, 534, 710
FspBI CTAG 6 cut(s) 467, 686, 786, 977, 1115, 1247
GluI GCNGC 5 cut(s) 93, 460, 504, 534, 710
GsaI CCCAGC 1 cut(s) 533
GsuI CTGGAG 2 cut(s) 1400, 1456
HaeIII GGCC 2 cut(s) 1027, 1339
HapII CCGG 2 cut(s) 59, 249
Hin1II CATG 6 cut(s) 76, 466, 552, 571, 851, 1635
HincII GTYRAC 1 cut(s) 1359
HindII GTYRAC 1 cut(s) 1359
HindIII AAGCTT 2 cut(s) 483, 1169
HinfI GANTC 5 cut(s) 734, 800, 907, 1500, 1616
HpaII CCGG 2 cut(s) 59, 249
HphI GGTGA 4 cut(s) 830, 1277, 1394, 1561
Hpy166II GTNNAC 7 cut(s) 64, 114, 169, 821, 1268, 1359, 1541
Hpy188I TCNGA 2 cut(s) 34, 540
Hpy188III TCNNGA 4 cut(s) 284, 731, 1133, 1228
Hpy8I GTNNAC 7 cut(s) 64, 114, 169, 821, 1268, 1359, 1541
HpyAV CCTTC 5 cut(s) 32, 114, 835, 1401, 1586
HpyCH4III ACNGT 1 cut(s) 1363
HpyCH4IV ACGT 1 cut(s) 1187
HpyF10VI GCNNNNNNNGC 8 cut(s) 89, 98, 350, 533, 709, 752, 1247, 1256
HpyF3I CTNAG 3 cut(s) 537, 1029, 1287
HpySE526I ACGT 1 cut(s) 1187
Hsp92II CATG 6 cut(s) 76, 466, 552, 571, 851, 1635
KpnI GGTACC 1 cut(s) 249
Kzo9I GATC 1 cut(s) 1066
LmnI GCTCC 5 cut(s) 100, 106, 176, 204, 511
Lsp1109I GCAGC 4 cut(s) 104, 515, 520, 721
LweI GCATC 3 cut(s) 353, 1051, 1411
MaeI CTAG 6 cut(s) 467, 686, 786, 977, 1115, 1247
MaeII ACGT 1 cut(s) 1187
MaeIII GTNAC 5 cut(s) 126, 623, 980, 1183, 1549
MalI GATC 1 cut(s) 1068
MbiI CCGCTC 1 cut(s) 101
MboI GATC 1 cut(s) 1066
MfeI CAATTG 1 cut(s) 1089
MflI RGATCY 1 cut(s) 1066
MhlI GDGCHC 1 cut(s) 1112
MluCI AATT 8 cut(s) 856, 886, 912, 1089, 1191, 1300, 1368, 1425
MmeI TCCRAC 4 cut(s) 295, 367, 598, 1387
MnlI CCTC 9 cut(s) 117, 167, 930, 1073, 1115, 1329, 1410, 1431, 1525
Mph1103I ATGCAT 1 cut(s) 646
MroXI GAANNNNTTC 1 cut(s) 777
MseI TTAA 7 cut(s) 150, 1167, 1190, 1308, 1323, 1428, 1527
MslI CAYNNNNRTG 1 cut(s) 71
MspA1I CMGCKG 3 cut(s) 533, 712, 1481
MspI CCGG 2 cut(s) 59, 249
MspR9I CCNGG 3 cut(s) 109, 250, 1418
MunI CAATTG 1 cut(s) 1089
MvaI CCWGG 2 cut(s) 109, 1418
MwoI GCNNNNNNNGC 8 cut(s) 89, 98, 350, 533, 709, 752, 1247, 1256
NciI CCSGG 1 cut(s) 250
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 2 cut(s) 796, 1053
NdeII GATC 1 cut(s) 1066
NlaIII CATG 6 cut(s) 76, 466, 552, 571, 851, 1635
NlaIV GGNNCC 6 cut(s) 206, 247, 1026, 1201, 1216, 1293
NmuCI GTSAC 2 cut(s) 623, 1549
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 3 cut(s) 466, 571, 851
NspV TTCGAA 1 cut(s) 812
PaeI GCATGC 1 cut(s) 466
PciI ACATGT 2 cut(s) 567, 847
PdmI GAANNNNTTC 1 cut(s) 777
PfeI GAWTC 5 cut(s) 734, 800, 907, 1500, 1616
PfoI TCCNGGA 1 cut(s) 1416
PkrI GCNGC 5 cut(s) 94, 461, 505, 535, 711
PpuMI RGGWCCY 1 cut(s) 1292
PscI ACATGT 2 cut(s) 567, 847
PshBI ATTAAT 1 cut(s) 1308
Psp1406I AACGTT 1 cut(s) 1187
Psp5II RGGWCCY 1 cut(s) 1292
Psp6I CCWGG 2 cut(s) 107, 1416
PspEI GGTNACC 1 cut(s) 1549
PspFI CCCAGC 1 cut(s) 529
PspGI CCWGG 2 cut(s) 107, 1416
PspN4I GGNNCC 6 cut(s) 206, 247, 1026, 1201, 1216, 1293
PspPI GGNCC 5 cut(s) 169, 402, 444, 1025, 1292
PspPPI RGGWCCY 1 cut(s) 1292
PsuI RGATCY 1 cut(s) 1066
PvuII CAGCTG 3 cut(s) 533, 712, 1481
RsaI GTAC 3 cut(s) 220, 247, 1389
RsaNI GTAC 3 cut(s) 219, 246, 1388
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 7 cut(s) 150, 1167, 1190, 1308, 1323, 1428, 1527
SatI GCNGC 5 cut(s) 93, 460, 504, 534, 710
Sau3AI GATC 1 cut(s) 1066
Sau96I GGNCC 5 cut(s) 169, 402, 444, 1025, 1292
ScrFI CCNGG 3 cut(s) 109, 250, 1418
SduI GDGCHC 1 cut(s) 1112
SfaNI GCATC 3 cut(s) 353, 1051, 1411
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 4 cut(s) 169, 402, 444, 1292
SmiMI CAYNNNNRTG 1 cut(s) 71
SphI GCATGC 1 cut(s) 466
Sse9I AATT 8 cut(s) 856, 886, 912, 1089, 1191, 1300, 1368, 1425
SsiI CCGC 6 cut(s) 90, 99, 460, 1098, 1118, 1395
SspI AATATT 1 cut(s) 1104
SspMI CTAG 6 cut(s) 467, 686, 786, 977, 1115, 1247
StyD4I CCNGG 3 cut(s) 107, 248, 1416
StyI CCWWGG 2 cut(s) 72, 208
TaaI ACNGT 1 cut(s) 1363
TaiI ACGT 1 cut(s) 1190
TaqI TCGA 2 cut(s) 435, 812
TaqII GACCGA 1 cut(s) 186
TasI AATT 8 cut(s) 856, 886, 912, 1089, 1191, 1300, 1368, 1425
TatI WGTACW 1 cut(s) 218
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 5 cut(s) 734, 800, 907, 1500, 1616
Tru1I TTAA 7 cut(s) 150, 1167, 1190, 1308, 1323, 1428, 1527
Tru9I TTAA 7 cut(s) 150, 1167, 1190, 1308, 1323, 1428, 1527
TscAI CASTG 3 cut(s) 753, 762, 1185
TseFI GTSAC 2 cut(s) 623, 1549
TseI GCWGC 4 cut(s) 92, 503, 533, 709
Tsp45I GTSAC 2 cut(s) 623, 1549
TspDTI ATGAA 6 cut(s) 420, 502, 713, 716, 854, 1361
TspRI CASTG 3 cut(s) 753, 762, 1185
VpaK11BI GGWCC 4 cut(s) 169, 402, 444, 1292
VspI ATTAAT 1 cut(s) 1308
XapI RAATTY 3 cut(s) 856, 886, 912
XceI RCATGY 3 cut(s) 466, 571, 851
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmnI GAANNNNTTC 1 cut(s) 777
XspI CTAG 6 cut(s) 467, 686, 786, 977, 1115, 1247
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.