pycom05g04300

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001054
Physical Location & Seq
Forward (+)
5038416 .. 5047855
9440 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04300.7

Sequence Viewer

Length: 1614 bp
ATGGCTCAAAAGGCAACAAAGTCTTCCGAAGAAGCCAAGCCCCGTCTGGCGGAGACAGCCATGGTTATTGCTTCAACAGCAGCTCCCGCTCCTTCCTGGTTCACGCCCAAGAGGTTACTTGTTATCTTTTGCTTGATTAACTTGATAAATTATGTGGACCGAGGAACAATAGCAAGCAATGGTGTCAATGGGAGCCGCACAACATGTACAGATAGTGGTATATGCACGGCTGGTACCGGGATACAGGGGGAGTTTCACTTGAACAACTTTCAAGATGGAGTTCTATCATCAGCTTTTATGGTTGGACTTCTTATTGCTTCTCCTGTATTTGCATCATTAGCAAAGAGTGTAAGTCCCTTTAGGCTCATTGGAGTTGGATTATCAGTTTGGACCTTTGCGATAGTTGGTTGTGCTTGTTCATTGGGTTTCTGGTCCATTACTATTTTCCGCATGCTAGTTGGTGTTGGTGAAGCTTCATTTATTAGTCTTGCAGCTCCATTCATTGACGATAATGCCCCAGCTGCTCAGAGAACTGCGTGGCTTGCTATATTCTACATGTGCATACCAAGTGGATATGCACTTGGCTATGTTTATGGTGGGGTGGTTGGAAGTCACACCAATTGGCGTTATGCATTTGTGGGGGAAGCAATCCTTATGCTACCATTTGCTATTCTAGGATTTGTTATGAAGCCTTTGCAGCTGAAAGGTTTTTTTCATCCTGAATCAAAAAAAGCAGTTACAGCAGTGGAAACGGCTGTTGAAGTTCAAGGTGCTGATACTTATGGTATTTCGAATGGTAAAGATGCTTCCTATTCGATGAAGAAAGATATGAGAAATTCCACCTTACAAAAATCTTCCAAGTCAAAGGTTGCATCAAACATAAAGAATCAAATATCAAGGTTTTTGAATGATATGAAGGTGCTGTTGGTGGATAAGGTTTATGTTGTGAATGTTCTAGGTTACATAGCATACAACTTTGTCATAGGTGCATACTCATATTGGGGACCTAAGGCGGGATATAATATATATCATATGAATGATGCAGATACAATATTTGGAGGTATTACAATCATGTCCGGAATATTGGGCACACTAGCGGGAGGCTTTCTTCTGGATTATATGACTAACACTATCTCTAATGCTTTTAAGCTTCTTTCAGCGGTAACGTTAATTGGTGGTGCCTTTTGCTTCGGTGCCTTTTGCTTCAAGAATATGTATGTTTTCCTAGCTCTTTTCGGAATCGGTGAACTACTTGTCTTTGCAACTCAGGGTCCTGTAAATTACATTTGTCTCCACTGTGTTAAGCCTAGTATGAGGCCGCTGTCTATGGCTATTTCTACTGTTTCAATTCATCTCTTCGGTGATGTACCTTCCGCACCTCTTGTTGGAGTTCTCCAGGATGCCATTAACAACTGGAGGAAGACTGCTCTTATTCTAACAGCTGTTTTCTTTCCATCAGCTCTAATATGGTTTATAGGAATCTTTCTGCACAGCGTGGATAGATTTAACGAGGAAAGTGAGCATCAGATCACCACCAAAACCGAAAGGTCAAGCACAACGCCATTGCTTGAAGGGAAGAAGTTGGAAACAACTGAATCTGTTGCTGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

538

Amino Acids

58.04

Weight (kDa)

8.66

Isoelectric Point (pI)

27.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 3 cut(s) 233, 1178, 1193
AccBSI CCGCTC 1 cut(s) 89
AccIII TCCGGA 1 cut(s) 1076
AciI CCGC 9 cut(s) 50, 87, 196, 448, 1013, 1097, 1160, 1319, 1374
AclI AACGTT 1 cut(s) 1166
AcsI RAATTY 1 cut(s) 835
AdeI CACNNNGTG 1 cut(s) 1495
AfaI GTAC 3 cut(s) 208, 235, 1368
AfiI CCNNNNNNNGG 3 cut(s) 49, 1013, 1385
AflIII ACRYGT 2 cut(s) 203, 555
AgsI TTSAA 9 cut(s) 75, 262, 272, 761, 767, 907, 1207, 1347, 1571
AjnI CCWGG 2 cut(s) 95, 1395
AjuI GAANNNNNNNTTGG 2 cut(s) 908, 940
Alw26I GTCTC 2 cut(s) 47, 1295
Aor13HI TCCGGA 1 cut(s) 1076
AoxI GGCC 1 cut(s) 1316
ApeKI GCWGC 4 cut(s) 80, 491, 521, 697
ApoI RAATTY 1 cut(s) 835
Asp718I GGTACC 1 cut(s) 233
AspS9I GGNCC 5 cut(s) 157, 390, 432, 1004, 1271
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 4 cut(s) 479, 1256, 1373, 1522
AsuII TTCGAA 1 cut(s) 791
AvaII GGWCC 5 cut(s) 157, 390, 432, 1004, 1271
AxyI CCTNAGG 1 cut(s) 1008
BaeGI GKGCMC 1 cut(s) 1091
BanI GGYRCC 3 cut(s) 233, 1178, 1193
BarI GAAGNNNNNNTAC 2 cut(s) 790, 822
BbsI GAAGAC 2 cut(s) 15, 1427
BbvI GCAGC 4 cut(s) 92, 503, 508, 709
BccI CCATC 2 cut(s) 269, 1462
BceAI ACGGC 2 cut(s) 243, 768
BciT130I CCWGG 2 cut(s) 97, 1397
BciVI GTATCC 1 cut(s) 234
BcnI CCSGG 1 cut(s) 238
BcoDI GTCTC 2 cut(s) 47, 1295
BfaI CTAG 6 cut(s) 455, 674, 956, 1094, 1226, 1308
BfuI GTATCC 1 cut(s) 234
BisI GCNGC 6 cut(s) 81, 196, 492, 522, 698, 1319
BlsI GCNGC 6 cut(s) 82, 197, 493, 523, 699, 1320
Bme1390I CCNGG 3 cut(s) 97, 238, 1397
Bme18I GGWCC 5 cut(s) 157, 390, 432, 1004, 1271
BmgT120I GGNCC 5 cut(s) 157, 390, 432, 1004, 1271
BmiI GGNNCC 6 cut(s) 194, 235, 1005, 1180, 1195, 1272
BmrFI CCNGG 3 cut(s) 97, 238, 1397
BmsI GCATC 6 cut(s) 341, 793, 881, 1030, 1390, 1531
BpiI GAAGAC 2 cut(s) 15, 1427
BpmI CTGGAG 2 cut(s) 1379, 1435
Bpu14I TTCGAA 1 cut(s) 791
BpuMI CCSGG 1 cut(s) 238
BsaJI CCNNGG 2 cut(s) 60, 160
BsaWI WCCGGW 1 cut(s) 1076
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 1013, 1385
Bse1I ACTGG 1 cut(s) 1418
Bse21I CCTNAGG 1 cut(s) 1008
Bse3DI GCAATG 2 cut(s) 184, 1562
BseAI TCCGGA 1 cut(s) 1076
BseBI CCWGG 2 cut(s) 97, 1397
BseDI CCNNGG 2 cut(s) 60, 160
BseGI GGATG 2 cut(s) 715, 1405
BseLI CCNNNNNNNGG 3 cut(s) 49, 1013, 1385
BseMI GCAATG 2 cut(s) 184, 1562
BseMII CTCAG 2 cut(s) 539, 1280
BseNI ACTGG 1 cut(s) 1418
BseSI GKGCMC 1 cut(s) 1091
BseXI GCAGC 4 cut(s) 92, 503, 508, 709
BseYI CCCAGC 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 1472
BshFI GGCC 1 cut(s) 1318
BshNI GGYRCC 3 cut(s) 233, 1178, 1193
BsiSI CCGG 2 cut(s) 237, 1077
BslFI GGGAC 2 cut(s) 339, 1017
BslI CCNNNNNNNGG 3 cut(s) 49, 1013, 1385
BsmAI GTCTC 2 cut(s) 47, 1295
BsmFI GGGAC 2 cut(s) 339, 1017
BsnI GGCC 1 cut(s) 1318
Bsp119I TTCGAA 1 cut(s) 791
Bsp1286I GDGCHC 1 cut(s) 1091
Bsp13I TCCGGA 1 cut(s) 1076
Bsp1407I TGTACA 1 cut(s) 206
Bsp143I GATC 1 cut(s) 1527
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 9 cut(s) 50, 87, 196, 448, 1013, 1097, 1160, 1319, 1374
BspANI GGCC 1 cut(s) 1318
BspCNI CTCAG 2 cut(s) 538, 1279
BspEI TCCGGA 1 cut(s) 1076
BspLI GGNNCC 6 cut(s) 194, 235, 1005, 1180, 1195, 1272
BspT104I TTCGAA 1 cut(s) 791
BspT107I GGYRCC 3 cut(s) 233, 1178, 1193
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 184, 1562
BsrGI TGTACA 1 cut(s) 206
BsrI ACTGG 1 cut(s) 1418
BssECI CCNNGG 2 cut(s) 60, 160
BssMI GATC 1 cut(s) 1527
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 97, 1397
Bst4CI ACNGT 2 cut(s) 1298, 1342
Bst6I CTCTTC 1 cut(s) 1361
BstAUI TGTACA 1 cut(s) 206
BstBI TTCGAA 1 cut(s) 791
BstC8I GCNNGC 3 cut(s) 175, 452, 543
BstDEI CTNAG 3 cut(s) 525, 1008, 1266
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 2 cut(s) 715, 1405
BstKTI GATC 1 cut(s) 1530
BstMAI GTCTC 2 cut(s) 47, 1295
BstMBI GATC 1 cut(s) 1527
BstMWI GCNNNNNNNGC 9 cut(s) 11, 56, 77, 86, 338, 521, 542, 697, 740
BstNI CCWGG 2 cut(s) 97, 1397
BstNSI RCATGY 3 cut(s) 207, 454, 559
BstSCI CCNGG 3 cut(s) 95, 236, 1395
BstSLI GKGCMC 1 cut(s) 1091
BstV1I GCAGC 4 cut(s) 92, 503, 508, 709
BstV2I GAAGAC 2 cut(s) 15, 1427
Bsu36I CCTNAGG 1 cut(s) 1008
BsuI GTATCC 1 cut(s) 234
BsuRI GGCC 1 cut(s) 1318
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 2 cut(s) 715, 1405
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 2 cut(s) 750, 1294
Cac8I GCNNGC 3 cut(s) 175, 452, 543
Cfr13I GGNCC 5 cut(s) 157, 390, 432, 1004, 1271
Csp6I GTAC 3 cut(s) 207, 234, 1367
CviAII CATG 5 cut(s) 61, 204, 451, 556, 1072
CviQI GTAC 3 cut(s) 207, 234, 1367
DdeI CTNAG 3 cut(s) 525, 1008, 1266
DpnI GATC 1 cut(s) 1529
DpnII GATC 1 cut(s) 1527
DraIII CACNNNGTG 1 cut(s) 1495
Eam1104I CTCTTC 1 cut(s) 1361
EarI CTCTTC 1 cut(s) 1361
EciI GGCGGA 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 60
Eco47I GGWCC 5 cut(s) 157, 390, 432, 1004, 1271
Eco81I CCTNAGG 1 cut(s) 1008
EcoO109I RGGNCCY 2 cut(s) 1004, 1271
EcoRII CCWGG 2 cut(s) 95, 1395
EcoT14I CCWWGG 1 cut(s) 60
EcoT22I ATGCAT 1 cut(s) 634
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 5 cut(s) 64, 207, 454, 559, 1075
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 2 cut(s) 339, 1017
FatI CATG 5 cut(s) 60, 203, 450, 555, 1071
FauI CCCGC 3 cut(s) 94, 1006, 1090
FauNDI CATATG 1 cut(s) 1032
Fnu4HI GCNGC 6 cut(s) 81, 196, 492, 522, 698, 1319
FokI GGATG 2 cut(s) 702, 1412
Fsp4HI GCNGC 6 cut(s) 81, 196, 492, 522, 698, 1319
FspBI CTAG 6 cut(s) 455, 674, 956, 1094, 1226, 1308
GluI GCNGC 6 cut(s) 81, 196, 492, 522, 698, 1319
GsaI CCCAGC 1 cut(s) 521
GsuI CTGGAG 2 cut(s) 1379, 1435
HaeIII GGCC 1 cut(s) 1318
HapII CCGG 2 cut(s) 237, 1077
Hin1II CATG 5 cut(s) 64, 207, 454, 559, 1075
HindIII AAGCTT 2 cut(s) 471, 1148
HinfI GANTC 5 cut(s) 722, 886, 1239, 1479, 1595
HpaII CCGG 2 cut(s) 237, 1077
HphI GGTGA 4 cut(s) 479, 1256, 1373, 1522
Hpy166II GTNNAC 3 cut(s) 102, 157, 1247
Hpy188I TCNGA 4 cut(s) 28, 528, 1238, 1527
Hpy188III TCNNGA 5 cut(s) 272, 719, 1077, 1112, 1207
Hpy8I GTNNAC 3 cut(s) 102, 157, 1247
HpyAV CCTTC 4 cut(s) 102, 910, 1380, 1565
HpyCH4III ACNGT 2 cut(s) 1298, 1342
HpyCH4IV ACGT 1 cut(s) 1166
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 56, 77, 86, 338, 521, 542, 697, 740
HpyF3I CTNAG 3 cut(s) 525, 1008, 1266
HpySE526I ACGT 1 cut(s) 1166
Hsp92II CATG 5 cut(s) 64, 207, 454, 559, 1075
Kpn2I TCCGGA 1 cut(s) 1076
KpnI GGTACC 1 cut(s) 237
Kzo9I GATC 1 cut(s) 1527
LmnI GCTCC 4 cut(s) 88, 94, 192, 499
Lsp1109I GCAGC 4 cut(s) 92, 503, 508, 709
LweI GCATC 6 cut(s) 341, 793, 881, 1030, 1390, 1531
MaeI CTAG 6 cut(s) 455, 674, 956, 1094, 1226, 1308
MaeII ACGT 1 cut(s) 1166
MaeIII GTNAC 5 cut(s) 114, 611, 736, 959, 1162
MalI GATC 1 cut(s) 1529
MbiI CCGCTC 1 cut(s) 89
MboI GATC 1 cut(s) 1527
MboII GAAGA 8 cut(s) 15, 41, 832, 846, 1100, 1348, 1432, 1588
MfeI CAATTG 1 cut(s) 619
MhlI GDGCHC 1 cut(s) 1091
MluCI AATT 6 cut(s) 148, 619, 835, 1170, 1279, 1347
MmeI TCCRAC 5 cut(s) 283, 355, 586, 1366, 1563
MnlI CCTC 8 cut(s) 105, 155, 1052, 1094, 1308, 1389, 1410, 1504
Mph1103I ATGCAT 1 cut(s) 634
MroI TCCGGA 1 cut(s) 1076
MseI TTAA 6 cut(s) 138, 1146, 1169, 1302, 1407, 1506
MslI CAYNNNNRTG 1 cut(s) 1035
MspA1I CMGCKG 5 cut(s) 521, 700, 1160, 1321, 1442
MspI CCGG 2 cut(s) 237, 1077
MspR9I CCNGG 3 cut(s) 97, 238, 1397
MunI CAATTG 1 cut(s) 619
MvaI CCWGG 2 cut(s) 97, 1397
MwoI GCNNNNNNNGC 9 cut(s) 11, 56, 77, 86, 338, 521, 542, 697, 740
NciI CCSGG 1 cut(s) 238
NcoI CCATGG 1 cut(s) 60
NdeI CATATG 1 cut(s) 1032
NdeII GATC 1 cut(s) 1527
NlaIII CATG 5 cut(s) 64, 207, 454, 559, 1075
NlaIV GGNNCC 6 cut(s) 194, 235, 1005, 1180, 1195, 1272
NmuCI GTSAC 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 634
NspI RCATGY 3 cut(s) 207, 454, 559
NspV TTCGAA 1 cut(s) 791
PaeI GCATGC 1 cut(s) 454
PciI ACATGT 2 cut(s) 203, 555
PfeI GAWTC 5 cut(s) 722, 886, 1239, 1479, 1595
PfoI TCCNGGA 1 cut(s) 1395
PkrI GCNGC 6 cut(s) 82, 197, 493, 523, 699, 1320
PpuMI RGGWCCY 2 cut(s) 1004, 1271
PscI ACATGT 2 cut(s) 203, 555
Psp1406I AACGTT 1 cut(s) 1166
Psp5II RGGWCCY 2 cut(s) 1004, 1271
Psp6I CCWGG 2 cut(s) 95, 1395
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 2 cut(s) 95, 1395
PspN4I GGNNCC 6 cut(s) 194, 235, 1005, 1180, 1195, 1272
PspPI GGNCC 5 cut(s) 157, 390, 432, 1004, 1271
PspPPI RGGWCCY 2 cut(s) 1004, 1271
PvuII CAGCTG 3 cut(s) 521, 700, 1442
RsaI GTAC 3 cut(s) 208, 235, 1368
RsaNI GTAC 3 cut(s) 207, 234, 1367
RseI CAYNNNNRTG 1 cut(s) 1035
SaqAI TTAA 6 cut(s) 138, 1146, 1169, 1302, 1407, 1506
SatI GCNGC 6 cut(s) 81, 196, 492, 522, 698, 1319
Sau3AI GATC 1 cut(s) 1527
Sau96I GGNCC 5 cut(s) 157, 390, 432, 1004, 1271
ScrFI CCNGG 3 cut(s) 97, 238, 1397
SduI GDGCHC 1 cut(s) 1091
SfaNI GCATC 6 cut(s) 341, 793, 881, 1030, 1390, 1531
SfuI TTCGAA 1 cut(s) 791
SinI GGWCC 5 cut(s) 157, 390, 432, 1004, 1271
SmiMI CAYNNNNRTG 1 cut(s) 1035
SphI GCATGC 1 cut(s) 454
Sse9I AATT 6 cut(s) 148, 619, 835, 1170, 1279, 1347
SsiI CCGC 9 cut(s) 50, 87, 196, 448, 1013, 1097, 1160, 1319, 1374
SspI AATATT 2 cut(s) 1053, 1083
SspMI CTAG 6 cut(s) 455, 674, 956, 1094, 1226, 1308
StyD4I CCNGG 3 cut(s) 95, 236, 1395
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 2 cut(s) 1298, 1342
TaiI ACGT 1 cut(s) 1169
TaqI TCGA 2 cut(s) 791, 815
TaqII GACCGA 1 cut(s) 174
TasI AATT 6 cut(s) 148, 619, 835, 1170, 1279, 1347
TatI WGTACW 1 cut(s) 206
TauI GCSGC 2 cut(s) 198, 1321
TfiI GAWTC 5 cut(s) 722, 886, 1239, 1479, 1595
Tru1I TTAA 6 cut(s) 138, 1146, 1169, 1302, 1407, 1506
Tru9I TTAA 6 cut(s) 138, 1146, 1169, 1302, 1407, 1506
TscAI CASTG 2 cut(s) 750, 1301
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 4 cut(s) 80, 491, 521, 697
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 9 cut(s) 408, 465, 490, 701, 704, 833, 929, 1049, 1340
TspRI CASTG 2 cut(s) 750, 1301
VpaK11BI GGWCC 5 cut(s) 157, 390, 432, 1004, 1271
XapI RAATTY 1 cut(s) 835
XceI RCATGY 3 cut(s) 207, 454, 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 43
XspI CTAG 6 cut(s) 455, 674, 956, 1094, 1226, 1308
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.