FvH4_6g42030

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001054
Physical Location & Seq
Forward (+)
32885999 .. 32888552
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42030.t1

Sequence Viewer

Length: 1458 bp
ATGGCGAATCAACAAGAAGATCAATCAAAGAAGCAGCCCTCGTGGTTCACGCCCAAGAGACTTCTCGTCATATTCTGTGTGATCAACTTGATCAACTATGTCGACCGAGGAGCAATAGCAAGCAACGGCGTCAATGGAAACCTACAGACTTGCAACAATGGCACGTGCACACCCGCTTCGGGCATACAAGGCGATTTTAAGTTGAACAACTTCAAAGATGGTTGGTTATCTTCTGCTTTCATGGTTGGACTTCTCGTGTCTTCTCCGATATTTGCATCCCTGGCAAAGAGTGTCAATCCTTTTCGTCTCATTGGAGTTGGGTTGGCAGTTTGGACTTTGGCCGTAACTGGCTGTGGTTTCTCATTCAACTTCTGGTCCATTATTATCTGCCGCATGCTGGTTGGTGTTGGTGAGGCTTCGTTTATTAGCCTTGCCGCGCCGTTCATCGATGATAATGCTCCACCTCCACAGAAAACCGCATGGCTTTCGATATTCTACATGTGCATACCATCTGGATATGCGCTTGGTTATGTTTATGGTGGCTTGGTGGGCACTAGGCTAAGTTGGCGTGCTGCATTTTGGGGGGAGGCAATTCTGATGCTTCCCTTTGTGATTCTCGGGTTTGTTGTCAAGCCTTTGCAGATGAGAGGCTTTAAGCGTGATGAATCAAAGGTTGCTGTTGCTGTCTCACAACTTCAAGATTCAGGTGCTTCTGAGACGAAAGCTGCAATGAAAATAAAGAAGCATCTTTCGAGGTTTATGAAAGATATGAAGGAGCTTTTGGTCGAAAAGGTGTACATTGTTAATGTTCTTGGTTATGTAGCATACACCTTTGTGATTGGTGCCTACTCATATTGGGGGCCTAAGGCTGGTTACAATATATATCATATGAAAAATGCAGATATGATGTTTGGAGGTGTTACAATTGTGTGCGGAGTACTCGGCACAGTAGCAGGAGGCTTTGTTCTGGATTATATGACCAACACTATCACCAATGCTTTCAAGCTTCTTGCAACCGCAACATTTATTGGGGCAATAATATGCTTTGGTGCCTTTTGCTTGAAGAACACGTATGGTTTCCTAGCTCTTTTTGCAGTTGGACAACTACTTGTCTTTGCAGTTCAGGGTCCTGTAAACTACATTTGTCTGCATTGTGTTAAACCTAGTTTAAGGCCACTGTCTATGGCCATTTCTACTGTTGCAATTCACATCTTTGGAGATGTGCCTTCCTCGCCTCTTGTAGGGGTTCTCCAGGATCACATCAAGAATTGGAGGACGACTGCTCTTATTCTAACATCAATTTTTATTCCGGCAGCTGTCATATGGTTTATCGGAATTTTTCTGCACAGCGAGGATAGGTACAATGAAGAAAGTGAGAAGCAGAAATCCACAACTGAGGTTGAAGAGAAGAAGGCGGAATCTACTGCTGAACCTGTCACATATTCAACTCTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

486

Amino Acids

53.03

Weight (kDa)

8.89

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 61 - 403 5.5e-33 Major Facilitator Superfamily
OATP PF03137 126 - 353 2.6e-21 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 352 - 420 6.1e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 842, 1049
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 437
AciI CCGC 7 cut(s) 174, 391, 435, 477, 933, 1017, 1415
AclWI GGATC 1 cut(s) 1263
AcoI YGGCCR 2 cut(s) 339, 1185
AcsI RAATTY 1 cut(s) 1335
AcvI CACGTG 1 cut(s) 165
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 3 cut(s) 797, 939, 1361
AfiI CCNNNNNNNGG 4 cut(s) 179, 397, 869, 1241
AflIII ACRYGT 2 cut(s) 498, 1068
AgsI TTSAA 8 cut(s) 205, 214, 367, 698, 1003, 1063, 1403, 1446
AhdI GACNNNNNGTC 1 cut(s) 65
AjnI CCWGG 2 cut(s) 279, 1251
AjuI GAANNNNNNNTTGG 2 cut(s) 764, 796
AleI CACNNNNGTG 1 cut(s) 833
AloI GAACNNNNNNTCC 2 cut(s) 948, 980
AluBI AGCT 5 cut(s) 725, 778, 1006, 1085, 1316
AluI AGCT 5 cut(s) 725, 778, 1006, 1085, 1316
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 4 cut(s) 52, 311, 691, 710
Alw44I GTGCAC 1 cut(s) 166
AlwI GGATC 1 cut(s) 1263
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 4 cut(s) 339, 860, 1172, 1185
ApaLI GTGCAC 1 cut(s) 166
ApeKI GCWGC 4 cut(s) 34, 572, 725, 1313
ApoI RAATTY 1 cut(s) 1335
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 2 cut(s) 439, 523
AspS9I GGNCC 3 cut(s) 375, 860, 1127
AsuHPI GGTGA 2 cut(s) 422, 982
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 2 cut(s) 375, 1127
AxyI CCTNAGG 1 cut(s) 864
BaeGI GKGCMC 2 cut(s) 170, 554
BalI TGGCCA 1 cut(s) 1187
BanI GGYRCC 2 cut(s) 842, 1049
BauI CACGAG 2 cut(s) 40, 254
BbrPI CACGTG 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 4 cut(s) 46, 559, 712, 1325
BccI CCATC 2 cut(s) 212, 517
BceAI ACGGC 3 cut(s) 142, 326, 424
BcgI CGANNNNNNTGC 4 cut(s) 437, 468, 471, 502
BciT130I CCWGG 2 cut(s) 281, 1253
BclI TGATCA 2 cut(s) 81, 90
BcoDI GTCTC 4 cut(s) 52, 311, 691, 710
BfaI CTAG 4 cut(s) 555, 1082, 1164, 1456
BfmI CTRYAG 1 cut(s) 143
BisI GCNGC 6 cut(s) 35, 391, 435, 573, 726, 1314
BlsI GCNGC 6 cut(s) 36, 392, 436, 574, 727, 1315
BmcAI AGTACT 1 cut(s) 939
Bme1390I CCNGG 2 cut(s) 281, 1253
Bme18I GGWCC 2 cut(s) 375, 1127
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 3 cut(s) 375, 860, 1127
BmiI GGNNCC 4 cut(s) 844, 861, 1051, 1128
BmrFI CCNGG 2 cut(s) 281, 1253
BmsI GCATC 3 cut(s) 284, 588, 754
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 1235
Bsa29I ATCGAT 1 cut(s) 447
BsaAI YACGTR 2 cut(s) 165, 1071
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 106, 279
BsaXI ACNNNNNCTCC 2 cut(s) 948, 978
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 397, 869, 1241
Bse1I ACTGG 1 cut(s) 352
Bse21I CCTNAGG 1 cut(s) 864
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 2 cut(s) 281, 1253
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 106, 279
BseGI GGATG 1 cut(s) 275
BseLI CCNNNNNNNGG 4 cut(s) 179, 397, 869, 1241
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 705, 1386
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 123
BseSI GKGCMC 2 cut(s) 170, 554
BseXI GCAGC 4 cut(s) 46, 559, 712, 1325
BsgI GTGCAG 1 cut(s) 1328
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 341, 862, 1174, 1187
BshNI GGYRCC 2 cut(s) 842, 1049
BshVI ATCGAT 1 cut(s) 447
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 170
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 1 cut(s) 1310
BslI CCNNNNNNNGG 4 cut(s) 179, 397, 869, 1241
BsmAI GTCTC 4 cut(s) 52, 311, 691, 710
BsmBI CGTCTC 2 cut(s) 311, 710
BsnI GGCC 4 cut(s) 341, 862, 1174, 1187
BsoBI CYCGRG 1 cut(s) 617
Bsp1286I GDGCHC 2 cut(s) 170, 554
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 4 cut(s) 19, 81, 90, 1255
BspACI CCGC 7 cut(s) 174, 391, 435, 477, 933, 1017, 1415
BspANI GGCC 4 cut(s) 341, 862, 1174, 1187
BspCNI CTCAG 2 cut(s) 706, 1387
BspDI ATCGAT 1 cut(s) 447
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 4 cut(s) 844, 861, 1051, 1128
BspPI GGATC 1 cut(s) 1263
BspT107I GGYRCC 2 cut(s) 842, 1049
BsrDI GCAATG 1 cut(s) 735
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 106, 279
BssMI GATC 4 cut(s) 19, 81, 90, 1255
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 2 cut(s) 40, 254
Bst2BI CACGAG 2 cut(s) 40, 254
Bst2UI CCWGG 2 cut(s) 281, 1253
Bst4CI ACNGT 3 cut(s) 949, 1179, 1198
Bst6I CTCTTC 1 cut(s) 1398
BstACI GRCGYC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 795
BstBAI YACGTR 2 cut(s) 165, 1071
BstC8I GCNNGC 3 cut(s) 121, 395, 570
BstDEI CTNAG 4 cut(s) 560, 714, 864, 1395
BstENI CCTNNNNNAGG 1 cut(s) 1239
BstF5I GGATG 1 cut(s) 275
BstFNI CGCG 1 cut(s) 437
BstHHI GCGC 2 cut(s) 439, 523
BstKTI GATC 4 cut(s) 22, 84, 93, 1258
BstMAI GTCTC 4 cut(s) 52, 311, 691, 710
BstMBI GATC 4 cut(s) 19, 81, 90, 1255
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 7 cut(s) 159, 189, 281, 549, 565, 1091, 1231
BstNI CCWGG 2 cut(s) 281, 1253
BstNSI RCATGY 2 cut(s) 397, 502
BstSCI CCNGG 2 cut(s) 279, 1251
BstSFI CTRYAG 1 cut(s) 143
BstSLI GKGCMC 2 cut(s) 170, 554
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 4 cut(s) 46, 559, 712, 1325
BstV2I GAAGAC 1 cut(s) 252
Bsu15I ATCGAT 1 cut(s) 447
Bsu36I CCTNAGG 1 cut(s) 864
BsuRI GGCC 4 cut(s) 341, 862, 1174, 1187
BsuTUI ATCGAT 1 cut(s) 447
BtsCI GGATG 1 cut(s) 275
BtsIMutI CAGTG 1 cut(s) 1175
Cac8I GCNNGC 3 cut(s) 121, 395, 570
CfoI GCGC 2 cut(s) 439, 523
Cfr13I GGNCC 3 cut(s) 375, 860, 1127
ClaI ATCGAT 1 cut(s) 447
CseI GACGC 1 cut(s) 118
Csp6I GTAC 3 cut(s) 796, 938, 1360
CviAII CATG 4 cut(s) 241, 394, 480, 499
CviQI GTAC 3 cut(s) 796, 938, 1360
DdeI CTNAG 4 cut(s) 560, 714, 864, 1395
DpnI GATC 4 cut(s) 21, 83, 92, 1257
DpnII GATC 4 cut(s) 19, 81, 90, 1255
DriI GACNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 2 cut(s) 339, 1185
Eam1104I CTCTTC 1 cut(s) 1398
Eam1105I GACNNNNNGTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 1398
EciI GGCGGA 1 cut(s) 1430
Eco47I GGWCC 2 cut(s) 375, 1127
Eco72I CACGTG 1 cut(s) 165
Eco81I CCTNAGG 1 cut(s) 864
Eco88I CYCGRG 1 cut(s) 617
EcoNI CCTNNNNNAGG 1 cut(s) 1239
EcoO109I RGGNCCY 2 cut(s) 860, 1127
EcoRII CCWGG 2 cut(s) 279, 1251
Esp3I CGTCTC 2 cut(s) 311, 710
FaeI CATG 4 cut(s) 244, 397, 483, 502
FatI CATG 4 cut(s) 240, 393, 479, 498
FauI CCCGC 1 cut(s) 181
FauNDI CATATG 2 cut(s) 888, 1322
FbaI TGATCA 2 cut(s) 81, 90
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 6 cut(s) 35, 391, 435, 573, 726, 1314
FokI GGATG 1 cut(s) 262
Fsp4HI GCNGC 6 cut(s) 35, 391, 435, 573, 726, 1314
FspBI CTAG 4 cut(s) 555, 1082, 1164, 1456
GlaI GCGC 2 cut(s) 438, 522
GluI GCNGC 6 cut(s) 35, 391, 435, 573, 726, 1314
GsuI CTGGAG 1 cut(s) 1235
HaeIII GGCC 4 cut(s) 341, 862, 1174, 1187
HapII CCGG 1 cut(s) 1310
HgaI GACGC 1 cut(s) 118
HhaI GCGC 2 cut(s) 439, 523
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 4 cut(s) 244, 397, 483, 502
Hin6I GCGC 2 cut(s) 437, 521
HinP1I GCGC 2 cut(s) 437, 521
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 1004
HinfI GANTC 5 cut(s) 7, 613, 665, 701, 1418
HpaII CCGG 1 cut(s) 1310
HphI GGTGA 2 cut(s) 422, 982
Hpy166II GTNNAC 5 cut(s) 48, 103, 168, 796, 1135
Hpy188I TCNGA 4 cut(s) 267, 597, 715, 1334
Hpy188III TCNNGA 4 cut(s) 513, 698, 968, 1264
Hpy8I GTNNAC 5 cut(s) 48, 103, 168, 796, 1135
HpyAV CCTTC 3 cut(s) 766, 1236, 1405
HpyCH4III ACNGT 3 cut(s) 949, 1179, 1198
HpyCH4IV ACGT 2 cut(s) 164, 1070
HpyF10VI GCNNNNNNNGC 7 cut(s) 159, 189, 281, 549, 565, 1091, 1231
HpyF3I CTNAG 4 cut(s) 560, 714, 864, 1395
HpySE526I ACGT 2 cut(s) 164, 1070
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 4 cut(s) 244, 397, 483, 502
HspAI GCGC 2 cut(s) 437, 521
Ksp22I TGATCA 2 cut(s) 81, 90
Kzo9I GATC 4 cut(s) 19, 81, 90, 1255
LmnI GCTCC 3 cut(s) 110, 463, 775
Lsp1109I GCAGC 4 cut(s) 46, 559, 712, 1325
LweI GCATC 3 cut(s) 284, 588, 754
MaeI CTAG 4 cut(s) 555, 1082, 1164, 1456
MaeII ACGT 2 cut(s) 164, 1070
MaeIII GTNAC 4 cut(s) 343, 872, 919, 1435
MalI GATC 4 cut(s) 21, 83, 92, 1257
MboI GATC 4 cut(s) 19, 81, 90, 1255
MboII GAAGA 7 cut(s) 29, 222, 252, 1075, 1379, 1415, 1420
MfeI CAATTG 1 cut(s) 924
MhlI GDGCHC 2 cut(s) 170, 554
MlsI TGGCCA 1 cut(s) 1187
MluCI AATT 6 cut(s) 591, 924, 1203, 1267, 1299, 1335
MluNI TGGCCA 1 cut(s) 1187
MmeI TCCRAC 2 cut(s) 226, 1078
Mox20I TGGCCA 1 cut(s) 1187
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 1187
MseI TTAA 5 cut(s) 198, 654, 804, 1158, 1169
MslI CAYNNNNRTG 1 cut(s) 833
Msp20I TGGCCA 1 cut(s) 1187
MspA1I CMGCKG 1 cut(s) 1316
MspI CCGG 1 cut(s) 1310
MspR9I CCNGG 2 cut(s) 281, 1253
MunI CAATTG 1 cut(s) 924
MvaI CCWGG 2 cut(s) 281, 1253
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 7 cut(s) 159, 189, 281, 549, 565, 1091, 1231
NdeI CATATG 2 cut(s) 888, 1322
NdeII GATC 4 cut(s) 19, 81, 90, 1255
NlaIII CATG 4 cut(s) 244, 397, 483, 502
NlaIV GGNNCC 4 cut(s) 844, 861, 1051, 1128
NmeAIII GCCGAG 1 cut(s) 921
NmuCI GTSAC 1 cut(s) 1435
NspI RCATGY 2 cut(s) 397, 502
OliI CACNNNNGTG 1 cut(s) 833
PaeI GCATGC 1 cut(s) 397
PciI ACATGT 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 5 cut(s) 7, 613, 665, 701, 1418
PfoI TCCNGGA 1 cut(s) 1251
PkrI GCNGC 6 cut(s) 36, 392, 436, 574, 727, 1315
PmaCI CACGTG 1 cut(s) 165
PmlI CACGTG 1 cut(s) 165
Ppu21I YACGTR 2 cut(s) 165, 1071
PpuMI RGGWCCY 1 cut(s) 1127
PscI ACATGT 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 1127
Psp6I CCWGG 2 cut(s) 279, 1251
PspCI CACGTG 1 cut(s) 165
PspGI CCWGG 2 cut(s) 279, 1251
PspN4I GGNNCC 4 cut(s) 844, 861, 1051, 1128
PspPI GGNCC 3 cut(s) 375, 860, 1127
PspPPI RGGWCCY 1 cut(s) 1127
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 3 cut(s) 797, 939, 1361
RsaNI GTAC 3 cut(s) 796, 938, 1360
RseI CAYNNNNRTG 1 cut(s) 833
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 5 cut(s) 198, 654, 804, 1158, 1169
SatI GCNGC 6 cut(s) 35, 391, 435, 573, 726, 1314
Sau3AI GATC 4 cut(s) 19, 81, 90, 1255
Sau96I GGNCC 3 cut(s) 375, 860, 1127
ScaI AGTACT 1 cut(s) 939
ScrFI CCNGG 2 cut(s) 281, 1253
SduI GDGCHC 2 cut(s) 170, 554
SfaNI GCATC 3 cut(s) 284, 588, 754
SfcI CTRYAG 1 cut(s) 143
SinI GGWCC 2 cut(s) 375, 1127
SmiMI CAYNNNNRTG 1 cut(s) 833
SphI GCATGC 1 cut(s) 397
Sse9I AATT 6 cut(s) 591, 924, 1203, 1267, 1299, 1335
SsiI CCGC 7 cut(s) 174, 391, 435, 477, 933, 1017, 1415
SspMI CTAG 4 cut(s) 555, 1082, 1164, 1456
StyD4I CCNGG 2 cut(s) 279, 1251
TaaI ACNGT 3 cut(s) 949, 1179, 1198
TaiI ACGT 2 cut(s) 167, 1073
TaqI TCGA 5 cut(s) 102, 447, 488, 752, 786
TaqII GACCGA 1 cut(s) 120
TasI AATT 6 cut(s) 591, 924, 1203, 1267, 1299, 1335
TatI WGTACW 2 cut(s) 795, 937
TauI GCSGC 2 cut(s) 393, 437
TfiI GAWTC 5 cut(s) 7, 613, 665, 701, 1418
Tru1I TTAA 5 cut(s) 198, 654, 804, 1158, 1169
Tru9I TTAA 5 cut(s) 198, 654, 804, 1158, 1169
TscAI CASTG 1 cut(s) 1182
TseFI GTSAC 1 cut(s) 1435
TseI GCWGC 4 cut(s) 34, 572, 725, 1313
Tsp45I GTSAC 1 cut(s) 1435
TspDTI ATGAA 8 cut(s) 229, 433, 678, 746, 776, 785, 905, 1380
TspRI CASTG 1 cut(s) 1182
VneI GTGCAC 1 cut(s) 166
VpaK11BI GGWCC 2 cut(s) 375, 1127
XagI CCTNNNNNAGG 1 cut(s) 1239
XapI RAATTY 1 cut(s) 1335
XceI RCATGY 2 cut(s) 397, 502
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 4 cut(s) 555, 1082, 1164, 1456
ZrmI AGTACT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.