Rh6BG171300

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001054
Physical Location & Seq
Reverse (-)
28255610 .. 28267353
11744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG171300.1

Sequence Viewer

Length: 1308 bp
ATGGTTGGACTTCTGGTGGCTTCTCCGATTTTTGCTTCATTAGCAAAGAGTGTGAATCCATTTAGGCTCATTGGAGTTGGATTGTCAGTTTGGACTTTTGCTGTAGTTGGTTGTGGTCTTTCATTCAACTTCTGGTCCATTACCATCTGCCGCATGCTAGTTGGTGTTGGCGAGGCTTCATTTATTAGTCTTGCAGCTCCGTTCATTGATGATAATGCCCCAGCTGCTCAGAAAACTGTGTGGCTTGCAATATTCTACATGTGCATACCATCTGGATATGCACTTGGTTATGTATATGGTGGGTTGGTGGGAAGTTATACCAATTGGCGTTTTGCATTCTGGGGGGAGGCAATCCTGATGCTTCCGTTTGCTATTCTAGGGTTTGTCATGAAGCCTTTGCAGCTTAAAGGTTTTATGCCTGCTGAATCAAAAGAAGTGCTGACAGGGGTGGAGACGGGTGTCTCAGAAGTTCAAGATTCAGGCGTTTCAAATGGTAAAGATGGTTTGTTTCCAGTGGAGGGAGATGTCAAATATTCTACCATACATAAACAGAATGAGGTGAACTTTGCAACAAAAATAAAGGGTCAAATTTCAAGGTTTACAAAAGATATGAAGGTGCTTTTGATTGAGAAGGTTTATGTTGTTAATGTTCTAGGTTACATAGCGTACAACTTTGTAATTGGTGCGTACTCATATTGGGGGCCTAAGGCTGGTTATAATATATATCATATGACTGATGCAGATTTGATATTTGGAGGTATCACAATTGTGTGTGGAATATTGGGTACCGTAGCAGGAGGCTTTCTTTTGGATTTCATGACCAGCAGTATCCCTAATGCTTTCAAGCTTCTTTCAGCAGCTACGTTTTTTGGGGCAATATTTTGCTTCAGTGCCTTTTGCTTCTCAAACATGTATGCTTTCCTAGCTCTATTTGCAGTTGGCGAACTACTTGTCTTTGCAACTCAGGGTCCTGTAAATTACATTTGTCTGCACTGTGTTAAACCTAGTATGAGACCACTAGCTATGGCCATGTCTACTGTTTCTATTCATCTTTTTGGGGATGTGCCTTCCTCACCTCTTGTCGGAGTTCTCGAGGATTGCATCAACAATTGGAGGGTGACTGCTCTTATTCTAACATCTATATTCATTCCAGCGGCTGGTATATGGTTTATAGGAATTTTTCTGCCTAGTGTGGATAGATTTAATGAAGAAAGTGAGAATCAGATATCCACAACTGAAAGGTCAAGCATAACTCCTTTGCTTGAAGGGACGAAGACAGAAACGACTGGATCCGCTGCTGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

435

Amino Acids

47.08

Weight (kDa)

5.48

Isoelectric Point (pI)

28.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 2 - 348 3.2e-25 Major Facilitator Superfamily
OATP PF03137 46 - 300 2.1e-17 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 298 - 366 1e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
Acc65I GGTACC 1 cut(s) 785
AccB1I GGYRCC 1 cut(s) 785
AccB7I CCANNNNNTGG 1 cut(s) 1157
AccI GTMKAC 1 cut(s) 1034
AciI CCGC 3 cut(s) 151, 1154, 1293
AclWI GGATC 2 cut(s) 1284, 1297
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 2 cut(s) 588, 1176
AcuI CTGAAG 1 cut(s) 871
AfaI GTAC 3 cut(s) 668, 689, 787
AfiI CCNNNNNNNGG 4 cut(s) 518, 710, 1082, 1157
AflIII ACRYGT 2 cut(s) 258, 909
AgsI TTSAA 6 cut(s) 127, 473, 489, 594, 844, 1265
AleI CACNNNNGTG 1 cut(s) 767
AluBI AGCT 7 cut(s) 197, 224, 403, 847, 860, 926, 1022
AluI AGCT 7 cut(s) 197, 224, 403, 847, 860, 926, 1022
Alw26I GTCTC 3 cut(s) 446, 466, 1006
AlwI GGATC 2 cut(s) 1284, 1297
AlwNI CAGNNNCTG 1 cut(s) 1157
Ama87I CYCGRG 1 cut(s) 1091
AoxI GGCC 2 cut(s) 701, 1026
ApeKI GCWGC 5 cut(s) 194, 224, 400, 857, 1295
ApoI RAATTY 2 cut(s) 588, 1176
ArsI GACNNNNNNTTYG 2 cut(s) 936, 968
Asp718I GGTACC 1 cut(s) 785
AspS9I GGNCC 3 cut(s) 135, 701, 968
AsuHPI GGTGA 3 cut(s) 571, 1065, 1129
AvaI CYCGRG 1 cut(s) 1091
AvaII GGWCC 2 cut(s) 135, 968
AxyI CCTNAGG 1 cut(s) 705
BalI TGGCCA 1 cut(s) 1028
BamHI GGATCC 1 cut(s) 1289
BanI GGYRCC 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 1280
BbvI GCAGC 5 cut(s) 206, 211, 412, 869, 1282
BccI CCATC 3 cut(s) 152, 277, 494
BciVI GTATCC 1 cut(s) 839
BcoDI GTCTC 3 cut(s) 446, 466, 1006
BfaI CTAG 7 cut(s) 158, 377, 653, 923, 1005, 1019, 1188
BfmI CTRYAG 1 cut(s) 102
BfuI GTATCC 1 cut(s) 839
BisI GCNGC 7 cut(s) 151, 195, 225, 401, 858, 1155, 1296
BlsI GCNGC 7 cut(s) 152, 196, 226, 402, 859, 1156, 1297
Bme18I GGWCC 2 cut(s) 135, 968
BmeT110I CYCGRG 1 cut(s) 1091
BmgT120I GGNCC 3 cut(s) 135, 701, 968
BmiI GGNNCC 4 cut(s) 702, 787, 969, 1291
BmsI GCATC 3 cut(s) 348, 727, 1110
BoxI GACNNNNGTC 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 1280
BsaI GGTCTC 1 cut(s) 1006
Bsc4I CCNNNNNNNGG 4 cut(s) 518, 710, 1082, 1157
Bse1I ACTGG 2 cut(s) 512, 1291
Bse21I CCTNAGG 1 cut(s) 705
BseGI GGATG 1 cut(s) 1066
BseLI CCNNNNNNNGG 4 cut(s) 518, 710, 1082, 1157
BseMII CTCAG 3 cut(s) 242, 477, 977
BseNI ACTGG 2 cut(s) 512, 1291
BseXI GCAGC 5 cut(s) 206, 211, 412, 869, 1282
BseYI CCCAGC 1 cut(s) 220
BsgI GTGCAG 1 cut(s) 974
BshFI GGCC 2 cut(s) 703, 1028
BshNI GGYRCC 1 cut(s) 785
BsiHKCI CYCGRG 1 cut(s) 1091
BslFI GGGAC 1 cut(s) 1282
BslI CCNNNNNNNGG 4 cut(s) 518, 710, 1082, 1157
BsmAI GTCTC 3 cut(s) 446, 466, 1006
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 1282
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 2 cut(s) 703, 1028
Bso31I GGTCTC 1 cut(s) 1006
BsoBI CYCGRG 1 cut(s) 1091
Bsp143I GATC 1 cut(s) 1289
BspACI CCGC 3 cut(s) 151, 1154, 1293
BspANI GGCC 2 cut(s) 703, 1028
BspCNI CTCAG 3 cut(s) 241, 476, 976
BspHI TCATGA 2 cut(s) 387, 816
BspLI GGNNCC 4 cut(s) 702, 787, 969, 1291
BspPI GGATC 2 cut(s) 1284, 1297
BspT107I GGYRCC 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 1006
BsrI ACTGG 2 cut(s) 512, 1291
BssMI GATC 1 cut(s) 1289
Bst4CI ACNGT 4 cut(s) 238, 790, 995, 1039
BstC8I GCNNGC 3 cut(s) 155, 246, 420
BstDEI CTNAG 4 cut(s) 228, 463, 705, 963
BstF5I GGATG 1 cut(s) 1066
BstKTI GATC 1 cut(s) 1292
BstMAI GTCTC 3 cut(s) 446, 466, 1006
BstMBI GATC 1 cut(s) 1289
BstMWI GCNNNNNNNGC 5 cut(s) 41, 224, 400, 923, 932
BstNSI RCATGY 3 cut(s) 157, 262, 913
BstPAI GACNNNNGTC 1 cut(s) 458
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 5 cut(s) 206, 211, 412, 869, 1282
BstV2I GAAGAC 1 cut(s) 1280
BstX2I RGATCY 1 cut(s) 1289
BstYI RGATCY 1 cut(s) 1289
Bsu36I CCTNAGG 1 cut(s) 705
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 2 cut(s) 703, 1028
BtsCI GGATG 1 cut(s) 1066
BtsIMutI CAGTG 3 cut(s) 519, 895, 991
Cac8I GCNNGC 3 cut(s) 155, 246, 420
CaiI CAGNNNCTG 1 cut(s) 1157
CciI TCATGA 2 cut(s) 387, 816
Cfr13I GGNCC 3 cut(s) 135, 701, 968
Csp6I GTAC 3 cut(s) 667, 688, 786
CviAII CATG 6 cut(s) 154, 259, 388, 817, 910, 1030
CviQI GTAC 3 cut(s) 667, 688, 786
DdeI CTNAG 4 cut(s) 228, 463, 705, 963
DpnI GATC 1 cut(s) 1291
DpnII GATC 1 cut(s) 1289
EaeI YGGCCR 1 cut(s) 1026
Eco31I GGTCTC 1 cut(s) 1006
Eco32I GATATC 1 cut(s) 1227
Eco47I GGWCC 2 cut(s) 135, 968
Eco57I CTGAAG 1 cut(s) 871
Eco81I CCTNAGG 1 cut(s) 705
Eco88I CYCGRG 1 cut(s) 1091
EcoO109I RGGNCCY 2 cut(s) 701, 968
EcoRV GATATC 1 cut(s) 1227
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 6 cut(s) 157, 262, 391, 820, 913, 1033
FaqI GGGAC 1 cut(s) 1282
FatI CATG 6 cut(s) 153, 258, 387, 816, 909, 1029
FauNDI CATATG 1 cut(s) 729
FblI GTMKAC 1 cut(s) 1034
Fnu4HI GCNGC 7 cut(s) 151, 195, 225, 401, 858, 1155, 1296
FokI GGATG 1 cut(s) 1073
Fsp4HI GCNGC 7 cut(s) 151, 195, 225, 401, 858, 1155, 1296
FspBI CTAG 7 cut(s) 158, 377, 653, 923, 1005, 1019, 1188
GluI GCNGC 7 cut(s) 151, 195, 225, 401, 858, 1155, 1296
GsaI CCCAGC 1 cut(s) 224
HaeIII GGCC 2 cut(s) 703, 1028
Hin1II CATG 6 cut(s) 157, 262, 391, 820, 913, 1033
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 4 cut(s) 55, 425, 476, 1219
HphI GGTGA 3 cut(s) 571, 1065, 1129
Hpy166II GTNNAC 3 cut(s) 562, 600, 1035
Hpy188I TCNGA 5 cut(s) 27, 231, 466, 1085, 1224
Hpy188III TCNNGA 6 cut(s) 273, 355, 388, 473, 817, 1091
Hpy8I GTNNAC 3 cut(s) 562, 600, 1035
HpyAV CCTTC 4 cut(s) 607, 625, 1077, 1259
HpyCH4III ACNGT 4 cut(s) 238, 790, 995, 1039
HpyCH4IV ACGT 1 cut(s) 863
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 224, 400, 923, 932
HpyF3I CTNAG 4 cut(s) 228, 463, 705, 963
HpySE526I ACGT 1 cut(s) 863
Hsp92II CATG 6 cut(s) 157, 262, 391, 820, 913, 1033
KpnI GGTACC 1 cut(s) 789
Kzo9I GATC 1 cut(s) 1289
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 5 cut(s) 206, 211, 412, 869, 1282
LweI GCATC 3 cut(s) 348, 727, 1110
MaeI CTAG 7 cut(s) 158, 377, 653, 923, 1005, 1019, 1188
MaeII ACGT 1 cut(s) 863
MaeIII GTNAC 2 cut(s) 656, 1117
MalI GATC 1 cut(s) 1291
MboI GATC 1 cut(s) 1289
MboII GAAGA 2 cut(s) 1220, 1285
MfeI CAATTG 3 cut(s) 322, 765, 1108
MflI RGATCY 1 cut(s) 1289
MlsI TGGCCA 1 cut(s) 1028
MluCI AATT 7 cut(s) 322, 588, 678, 765, 976, 1108, 1176
MluNI TGGCCA 1 cut(s) 1028
MmeI TCCRAC 2 cut(s) 58, 1063
Mox20I TGGCCA 1 cut(s) 1028
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 4 cut(s) 405, 645, 999, 1203
MslI CAYNNNNRTG 1 cut(s) 767
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 3 cut(s) 224, 1154, 1295
MunI CAATTG 3 cut(s) 322, 765, 1108
Mva1269I GAATGC 1 cut(s) 335
MwoI GCNNNNNNNGC 5 cut(s) 41, 224, 400, 923, 932
NdeI CATATG 1 cut(s) 729
NdeII GATC 1 cut(s) 1289
NlaIII CATG 6 cut(s) 157, 262, 391, 820, 913, 1033
NlaIV GGNNCC 4 cut(s) 702, 787, 969, 1291
NmuCI GTSAC 1 cut(s) 1117
NspI RCATGY 3 cut(s) 157, 262, 913
OliI CACNNNNGTG 1 cut(s) 767
PaeI GCATGC 1 cut(s) 157
PaeR7I CTCGAG 1 cut(s) 1091
PagI TCATGA 2 cut(s) 387, 816
PciI ACATGT 2 cut(s) 258, 909
PcsI WCGNNNNNNNCGW 1 cut(s) 1089
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 4 cut(s) 55, 425, 476, 1219
PflMI CCANNNNNTGG 1 cut(s) 1157
PkrI GCNGC 7 cut(s) 152, 196, 226, 402, 859, 1156, 1297
PpuMI RGGWCCY 1 cut(s) 968
PscI ACATGT 2 cut(s) 258, 909
PshAI GACNNNNGTC 1 cut(s) 458
PsiI TTATAA 1 cut(s) 717
Psp5II RGGWCCY 1 cut(s) 968
PspFI CCCAGC 1 cut(s) 220
PspN4I GGNNCC 4 cut(s) 702, 787, 969, 1291
PspPI GGNCC 3 cut(s) 135, 701, 968
PspPPI RGGWCCY 1 cut(s) 968
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 1 cut(s) 1289
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 3 cut(s) 668, 689, 787
RsaNI GTAC 3 cut(s) 667, 688, 786
RseI CAYNNNNRTG 1 cut(s) 767
SaqAI TTAA 4 cut(s) 405, 645, 999, 1203
SatI GCNGC 7 cut(s) 151, 195, 225, 401, 858, 1155, 1296
Sau3AI GATC 1 cut(s) 1289
Sau96I GGNCC 3 cut(s) 135, 701, 968
SfaNI GCATC 3 cut(s) 348, 727, 1110
SfcI CTRYAG 1 cut(s) 102
Sfr274I CTCGAG 1 cut(s) 1091
SinI GGWCC 2 cut(s) 135, 968
SlaI CTCGAG 1 cut(s) 1091
SmiMI CAYNNNNRTG 1 cut(s) 767
SmlI CTYRAG 1 cut(s) 1091
SmoI CTYRAG 1 cut(s) 1091
SphI GCATGC 1 cut(s) 157
Sse9I AATT 7 cut(s) 322, 588, 678, 765, 976, 1108, 1176
SsiI CCGC 3 cut(s) 151, 1154, 1293
SspI AATATT 4 cut(s) 252, 533, 780, 879
SspMI CTAG 7 cut(s) 158, 377, 653, 923, 1005, 1019, 1188
TaaI ACNGT 4 cut(s) 238, 790, 995, 1039
TaiI ACGT 1 cut(s) 866
TaqI TCGA 1 cut(s) 1092
TasI AATT 7 cut(s) 322, 588, 678, 765, 976, 1108, 1176
TauI GCSGC 2 cut(s) 153, 1157
TfiI GAWTC 4 cut(s) 55, 425, 476, 1219
Tru1I TTAA 4 cut(s) 405, 645, 999, 1203
Tru9I TTAA 4 cut(s) 405, 645, 999, 1203
TscAI CASTG 3 cut(s) 519, 895, 998
TseFI GTSAC 1 cut(s) 1117
TseI GCWGC 5 cut(s) 194, 224, 400, 857, 1295
Tsp45I GTSAC 1 cut(s) 1117
TspGWI ACGGA 2 cut(s) 189, 354
TspRI CASTG 3 cut(s) 519, 895, 998
Van91I CCANNNNNTGG 1 cut(s) 1157
VpaK11BI GGWCC 2 cut(s) 135, 968
XapI RAATTY 2 cut(s) 588, 1176
XceI RCATGY 3 cut(s) 157, 262, 913
XhoI CTCGAG 1 cut(s) 1091
XmiI GTMKAC 1 cut(s) 1034
XspI CTAG 7 cut(s) 158, 377, 653, 923, 1005, 1019, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.