RchiOBHm_Chr6g0269161

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001054
Physical Location & Seq
Reverse (-)
26366047 .. 26376399
10353 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24144

Sequence Viewer

Length: 1545 bp
ATGATCGTTTCTTCAATCGCACCTCTTCAGCCTTCATGGTTTTCTCCCAAGAAGTTACTTGTTGTATTTTGTGTGATTAACTTGATAAATTATGTGGACCGAGGAGCAATTGCAAGCAATGGTGTGAATGGGAGTCTGGGAACTTGTACAGAGGGTGGTACGTGCACATCTGGCACCGGCATACAGGGGGATTTTGACTTGAGCAATTTTGAAGATGGATGCCTATCATCCGCTTTTATGGTTGGACTTCTGGTGGCTTCTCCGATTTTTGCTTCATTAGCAAAGAGTGTGAATCCATTTAGGCTCATTGGAGTTGGATTGTCAGTTTGGACTTTTGCTGTAGTTGGTTGTGGTCTTTCATTCAACTTCTGGTCCATTACCATCTGCCGCATGCTAGTTGGTGTTGGCGAGGCTTCATTTATTAGTCTTGCAGCTCCGTTCATTGATGATAATGCCCCAGCTGCTCAGAAAACTGTGTGGCTTGCAATATTCTATATGTGCATACCATCTGGATATGCACTTGGTTATGTATATGGTGGGTTGGTGGGAAGTTATACCAATTGGCGTTTTGCATTCTGGGGGGAGGCAATCCTGATGCTTCCGTTTGCTATTCTAGGGTTTGTCATGAAGCCTTTGCAGCTTAAAGGTTTTATGCCTGCTGAATCAAAAGAAGTGCTGACAGGGATGGAGACGGGTGTCTCAGAAGTTCAAGATTCAGGCGTTTCGAATGGTAAAGATGGTTTGTTTCCAGTGGAGGGAGATGTCAAATATTCTACCATACATAAACAGAATGAGGTGAACTTTGCAACAAAAATAAAGGGTCAAATTTCAAGGTTTACAAAAGATATGAAGGTGCTTTTGATTGAGAAGGTTTATGTTGTTAATGTTCTAGGTTACATAGCGTACAACTTTGTAATTGGTGCGTACTCATATTGGGGGCCTAAGGCTGGTTATAATATATATCATATGACTGATGCAGATTTGATATTTGGAGGTATTACAATTGTGTGTGGAATATTGGGTACCGTAGCAGGAGGCTTTCTTTTGGATTTCATGACCAGCAGTATCCCTAATGCTTTCAAGCTTCTTTCAGCAGCTACGTTTTTTGGGGCAATATTTTGCTTCACTGCCTTTTGCTTCTCAAACATGTATGCTTTCCTAGCTCTATTTGCAGTTGGCGAACTACTTGTCTTTGCAACTCAGGGTCCTGTAAATTACATTTGTCTGCACTGTGTTAAACCTAGTATGAGACCACTAGCTATGGCCATGTCTACTGTTTCTATTCATCTTTTTGGGGATGTGCCTTCCTCACCTCTTGTCGGAGTTCTCGAGGATTGCATCAACAATTGGAGGGTGACTGCTCTTATTCTAACATCTATATTCATTCCAGCGGCTGGTATATGGTTTATAGGAATTTTTCTGCCTAGTGTGGATAGATTTAATGAAGAAAGCGAGAATCAGATATCCACAACTGAAAGGTCAAGCATAACTCCTTTGCTTGAAGGGACGAAGACAGAAACGACTGAATCCGCTGCTGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

514

Amino Acids

55.38

Weight (kDa)

5.07

Isoelectric Point (pI)

29.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 60 - 427 3.1e-29 Major Facilitator Superfamily
OATP PF03137 125 - 379 4.3e-17 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 378 - 445 1.3e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 954
Acc65I GGTACC 1 cut(s) 1022
AccB1I GGYRCC 2 cut(s) 173, 1022
AccB7I CCANNNNNTGG 1 cut(s) 1394
AccI GTMKAC 1 cut(s) 1271
AciI CCGC 4 cut(s) 231, 388, 1391, 1530
AcoI YGGCCR 1 cut(s) 1263
AcsI RAATTY 2 cut(s) 825, 1413
AcuI CTGAAG 1 cut(s) 11
AfaI GTAC 5 cut(s) 148, 160, 905, 926, 1024
AfiI CCNNNNNNNGG 4 cut(s) 755, 947, 1319, 1394
AflIII ACRYGT 1 cut(s) 1146
AgsI TTSAA 7 cut(s) 15, 212, 364, 710, 831, 1081, 1502
AluBI AGCT 7 cut(s) 434, 461, 640, 1084, 1097, 1163, 1259
AluI AGCT 7 cut(s) 434, 461, 640, 1084, 1097, 1163, 1259
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 3 cut(s) 683, 703, 1243
Alw44I GTGCAC 1 cut(s) 163
AlwNI CAGNNNCTG 1 cut(s) 1394
Ama87I CYCGRG 1 cut(s) 1328
AoxI GGCC 2 cut(s) 938, 1263
ApaLI GTGCAC 1 cut(s) 163
ApeKI GCWGC 5 cut(s) 431, 461, 637, 1094, 1532
ApoI RAATTY 2 cut(s) 825, 1413
ArsI GACNNNNNNTTYG 2 cut(s) 1173, 1205
Asp718I GGTACC 1 cut(s) 1022
AspS9I GGNCC 4 cut(s) 97, 372, 938, 1205
AsuHPI GGTGA 3 cut(s) 808, 1302, 1366
AsuII TTCGAA 1 cut(s) 725
AvaI CYCGRG 1 cut(s) 1328
AvaII GGWCC 3 cut(s) 97, 372, 1205
AxyI CCTNAGG 1 cut(s) 942
BaeGI GKGCMC 1 cut(s) 167
BalI TGGCCA 1 cut(s) 1265
BanI GGYRCC 2 cut(s) 173, 1022
BbsI GAAGAC 1 cut(s) 1517
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 5 cut(s) 443, 448, 649, 1106, 1519
BccI CCATC 5 cut(s) 209, 389, 514, 679, 731
BciVI GTATCC 1 cut(s) 1076
BcoDI GTCTC 3 cut(s) 683, 703, 1243
BfaI CTAG 7 cut(s) 395, 614, 890, 1160, 1242, 1256, 1425
BfmI CTRYAG 1 cut(s) 339
BfuI GTATCC 1 cut(s) 1076
BisI GCNGC 7 cut(s) 388, 432, 462, 638, 1095, 1392, 1533
BlsI GCNGC 7 cut(s) 389, 433, 463, 639, 1096, 1393, 1534
Bme18I GGWCC 3 cut(s) 97, 372, 1205
BmeT110I CYCGRG 1 cut(s) 1328
BmgT120I GGNCC 4 cut(s) 97, 372, 938, 1205
BmiI GGNNCC 4 cut(s) 175, 939, 1024, 1206
BmsI GCATC 4 cut(s) 209, 585, 964, 1347
BoxI GACNNNNGTC 1 cut(s) 695
BpiI GAAGAC 1 cut(s) 1517
Bpu14I TTCGAA 1 cut(s) 725
BpuEI CTTGAG 1 cut(s) 220
BsaAI YACGTR 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 1243
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 4 cut(s) 755, 947, 1319, 1394
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 749
Bse21I CCTNAGG 1 cut(s) 942
Bse3DI GCAATG 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 4 cut(s) 224, 227, 690, 1303
BseJI GATNNNNATC 1 cut(s) 223
BseLI CCNNNNNNNGG 4 cut(s) 755, 947, 1319, 1394
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 3 cut(s) 479, 714, 1214
BseNI ACTGG 1 cut(s) 749
BseRI GAGGAG 1 cut(s) 117
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 5 cut(s) 443, 448, 649, 1106, 1519
BseYI CCCAGC 1 cut(s) 457
BsgI GTGCAG 1 cut(s) 1211
BshFI GGCC 2 cut(s) 940, 1265
BshNI GGYRCC 2 cut(s) 173, 1022
BsiHKAI GWGCWC 1 cut(s) 167
BsiHKCI CYCGRG 1 cut(s) 1328
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 1519
BslI CCNNNNNNNGG 4 cut(s) 755, 947, 1319, 1394
BsmAI GTCTC 3 cut(s) 683, 703, 1243
BsmBI CGTCTC 1 cut(s) 683
BsmFI GGGAC 1 cut(s) 1519
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 2 cut(s) 940, 1265
Bso31I GGTCTC 1 cut(s) 1243
BsoBI CYCGRG 1 cut(s) 1328
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 167
Bsp1407I TGTACA 1 cut(s) 146
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 4 cut(s) 231, 388, 1391, 1530
BspANI GGCC 2 cut(s) 940, 1265
BspCNI CTCAG 3 cut(s) 478, 713, 1213
BspHI TCATGA 2 cut(s) 624, 1053
BspLI GGNNCC 4 cut(s) 175, 939, 1024, 1206
BspT104I TTCGAA 1 cut(s) 725
BspT107I GGYRCC 2 cut(s) 173, 1022
BspTNI GGTCTC 1 cut(s) 1243
BsrDI GCAATG 1 cut(s) 124
BsrFI RCCGGY 1 cut(s) 176
BsrGI TGTACA 1 cut(s) 146
BsrI ACTGG 1 cut(s) 749
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 1 cut(s) 3
Bst4CI ACNGT 4 cut(s) 475, 1027, 1232, 1276
Bst6I CTCTTC 1 cut(s) 30
BstAUI TGTACA 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 162
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 4 cut(s) 115, 392, 483, 657
BstDEI CTNAG 4 cut(s) 465, 700, 942, 1200
BstF5I GGATG 4 cut(s) 224, 227, 690, 1303
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 3 cut(s) 683, 703, 1243
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 6 cut(s) 171, 278, 461, 637, 1160, 1169
BstNSI RCATGY 2 cut(s) 394, 1150
BstPAI GACNNNNGTC 1 cut(s) 695
BstSFI CTRYAG 1 cut(s) 339
BstSLI GKGCMC 1 cut(s) 167
BstV1I GCAGC 5 cut(s) 443, 448, 649, 1106, 1519
BstV2I GAAGAC 1 cut(s) 1517
Bsu36I CCTNAGG 1 cut(s) 942
BsuI GTATCC 1 cut(s) 1076
BsuRI GGCC 2 cut(s) 940, 1265
BtsCI GGATG 4 cut(s) 224, 227, 690, 1303
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 3 cut(s) 756, 1125, 1228
Cac8I GCNNGC 4 cut(s) 115, 392, 483, 657
CaiI CAGNNNCTG 1 cut(s) 1394
CciI TCATGA 2 cut(s) 624, 1053
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 4 cut(s) 97, 372, 938, 1205
Csp6I GTAC 5 cut(s) 147, 159, 904, 925, 1023
CviAII CATG 6 cut(s) 36, 391, 625, 1054, 1147, 1267
CviQI GTAC 5 cut(s) 147, 159, 904, 925, 1023
DdeI CTNAG 4 cut(s) 465, 700, 942, 1200
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
EaeI YGGCCR 1 cut(s) 1263
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 1243
Eco32I GATATC 1 cut(s) 1464
Eco47I GGWCC 3 cut(s) 97, 372, 1205
Eco57I CTGAAG 1 cut(s) 11
Eco81I CCTNAGG 1 cut(s) 942
Eco88I CYCGRG 1 cut(s) 1328
EcoO109I RGGNCCY 2 cut(s) 938, 1205
EcoRV GATATC 1 cut(s) 1464
Esp3I CGTCTC 1 cut(s) 683
FaeI CATG 6 cut(s) 39, 394, 628, 1057, 1150, 1270
FaqI GGGAC 1 cut(s) 1519
FatI CATG 6 cut(s) 35, 390, 624, 1053, 1146, 1266
FauNDI CATATG 1 cut(s) 966
FblI GTMKAC 1 cut(s) 1271
Fnu4HI GCNGC 7 cut(s) 388, 432, 462, 638, 1095, 1392, 1533
FokI GGATG 4 cut(s) 214, 231, 697, 1310
Fsp4HI GCNGC 7 cut(s) 388, 432, 462, 638, 1095, 1392, 1533
FspBI CTAG 7 cut(s) 395, 614, 890, 1160, 1242, 1256, 1425
GluI GCNGC 7 cut(s) 388, 432, 462, 638, 1095, 1392, 1533
GsaI CCCAGC 1 cut(s) 461
HaeIII GGCC 2 cut(s) 940, 1265
HapII CCGG 1 cut(s) 177
Hin1II CATG 6 cut(s) 39, 394, 628, 1057, 1150, 1270
HindIII AAGCTT 1 cut(s) 1082
HinfI GANTC 6 cut(s) 133, 292, 662, 713, 1456, 1526
HpaII CCGG 1 cut(s) 177
HphI GGTGA 3 cut(s) 808, 1302, 1366
Hpy166II GTNNAC 5 cut(s) 97, 165, 799, 837, 1272
Hpy188I TCNGA 5 cut(s) 264, 468, 703, 1322, 1461
Hpy188III TCNNGA 6 cut(s) 510, 592, 625, 710, 1054, 1328
Hpy8I GTNNAC 5 cut(s) 97, 165, 799, 837, 1272
HpyAV CCTTC 5 cut(s) 42, 844, 862, 1314, 1496
HpyCH4III ACNGT 4 cut(s) 475, 1027, 1232, 1276
HpyCH4IV ACGT 2 cut(s) 161, 1100
HpyF10VI GCNNNNNNNGC 6 cut(s) 171, 278, 461, 637, 1160, 1169
HpyF3I CTNAG 4 cut(s) 465, 700, 942, 1200
HpySE526I ACGT 2 cut(s) 161, 1100
Hsp92II CATG 6 cut(s) 39, 394, 628, 1057, 1150, 1270
KpnI GGTACC 1 cut(s) 1026
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 2 cut(s) 104, 439
Lsp1109I GCAGC 5 cut(s) 443, 448, 649, 1106, 1519
LweI GCATC 4 cut(s) 209, 585, 964, 1347
MaeI CTAG 7 cut(s) 395, 614, 890, 1160, 1242, 1256, 1425
MaeII ACGT 2 cut(s) 161, 1100
MaeIII GTNAC 3 cut(s) 54, 893, 1354
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 5 cut(s) 3, 17, 224, 1457, 1522
MfeI CAATTG 4 cut(s) 108, 559, 1002, 1345
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 1265
MluNI TGGCCA 1 cut(s) 1265
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 3 cut(s) 223, 295, 1300
Mox20I TGGCCA 1 cut(s) 1265
MscI TGGCCA 1 cut(s) 1265
MseI TTAA 5 cut(s) 78, 642, 882, 1236, 1440
Msp20I TGGCCA 1 cut(s) 1265
MspA1I CMGCKG 3 cut(s) 461, 1391, 1532
MspI CCGG 1 cut(s) 177
MunI CAATTG 4 cut(s) 108, 559, 1002, 1345
Mva1269I GAATGC 1 cut(s) 572
MwoI GCNNNNNNNGC 6 cut(s) 171, 278, 461, 637, 1160, 1169
NdeI CATATG 1 cut(s) 966
NdeII GATC 1 cut(s) 3
NlaIII CATG 6 cut(s) 39, 394, 628, 1057, 1150, 1270
NlaIV GGNNCC 4 cut(s) 175, 939, 1024, 1206
NmuCI GTSAC 1 cut(s) 1354
NspI RCATGY 2 cut(s) 394, 1150
NspV TTCGAA 1 cut(s) 725
PaeI GCATGC 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 1328
PagI TCATGA 2 cut(s) 624, 1053
PciI ACATGT 1 cut(s) 1146
PcsI WCGNNNNNNNCGW 1 cut(s) 1326
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 5 cut(s) 292, 662, 713, 1456, 1526
PflMI CCANNNNNTGG 1 cut(s) 1394
PkrI GCNGC 7 cut(s) 389, 433, 463, 639, 1096, 1393, 1534
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Ppu21I YACGTR 1 cut(s) 162
PpuMI RGGWCCY 1 cut(s) 1205
PscI ACATGT 1 cut(s) 1146
PshAI GACNNNNGTC 1 cut(s) 695
PsiI TTATAA 1 cut(s) 954
Psp5II RGGWCCY 1 cut(s) 1205
PspFI CCCAGC 1 cut(s) 457
PspN4I GGNNCC 4 cut(s) 175, 939, 1024, 1206
PspPI GGNCC 4 cut(s) 97, 372, 938, 1205
PspPPI RGGWCCY 1 cut(s) 1205
PstNI CAGNNNCTG 1 cut(s) 1394
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 5 cut(s) 148, 160, 905, 926, 1024
RsaNI GTAC 5 cut(s) 147, 159, 904, 925, 1023
SaqAI TTAA 5 cut(s) 78, 642, 882, 1236, 1440
SatI GCNGC 7 cut(s) 388, 432, 462, 638, 1095, 1392, 1533
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 4 cut(s) 97, 372, 938, 1205
SchI GAGTC 1 cut(s) 142
SduI GDGCHC 1 cut(s) 167
SfaNI GCATC 4 cut(s) 209, 585, 964, 1347
SfcI CTRYAG 1 cut(s) 339
Sfr274I CTCGAG 1 cut(s) 1328
SfuI TTCGAA 1 cut(s) 725
SinI GGWCC 3 cut(s) 97, 372, 1205
SlaI CTCGAG 1 cut(s) 1328
SmlI CTYRAG 2 cut(s) 199, 1328
SmoI CTYRAG 2 cut(s) 199, 1328
SphI GCATGC 1 cut(s) 394
SsiI CCGC 4 cut(s) 231, 388, 1391, 1530
SspI AATATT 4 cut(s) 489, 770, 1017, 1116
SspMI CTAG 7 cut(s) 395, 614, 890, 1160, 1242, 1256, 1425
TaaI ACNGT 4 cut(s) 475, 1027, 1232, 1276
TaiI ACGT 2 cut(s) 164, 1103
TaqI TCGA 2 cut(s) 725, 1329
TaqII GACCGA 1 cut(s) 114
TatI WGTACW 1 cut(s) 146
TauI GCSGC 2 cut(s) 390, 1394
TfiI GAWTC 5 cut(s) 292, 662, 713, 1456, 1526
Tru1I TTAA 5 cut(s) 78, 642, 882, 1236, 1440
Tru9I TTAA 5 cut(s) 78, 642, 882, 1236, 1440
TscAI CASTG 3 cut(s) 756, 1132, 1235
TseFI GTSAC 1 cut(s) 1354
TseI GCWGC 5 cut(s) 431, 461, 637, 1094, 1532
Tsp45I GTSAC 1 cut(s) 1354
TspGWI ACGGA 2 cut(s) 426, 591
TspRI CASTG 3 cut(s) 756, 1132, 1235
Van91I CCANNNNNTGG 1 cut(s) 1394
VneI GTGCAC 1 cut(s) 163
VpaK11BI GGWCC 3 cut(s) 97, 372, 1205
XapI RAATTY 2 cut(s) 825, 1413
XceI RCATGY 2 cut(s) 394, 1150
XhoI CTCGAG 1 cut(s) 1328
XmiI GTMKAC 1 cut(s) 1271
XspI CTAG 7 cut(s) 395, 614, 890, 1160, 1242, 1256, 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.