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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 115 |
| AgsI | TTSAA | 2 cut(s) | 133, 148 |
| BanI | GGYRCC | 1 cut(s) | 115 |
| BccI | CCATC | 1 cut(s) | 106 |
| BfaI | CTAG | 1 cut(s) | 11 |
| BmiI | GGNNCC | 1 cut(s) | 117 |
| BmsI | GCATC | 1 cut(s) | 51 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 88, 118 |
| BseRI | GAGGAG | 1 cut(s) | 12 |
| BshNI | GGYRCC | 1 cut(s) | 115 |
| BspLI | GGNNCC | 1 cut(s) | 117 |
| BspT107I | GGYRCC | 1 cut(s) | 115 |
| BstDEI | CTNAG | 1 cut(s) | 89 |
| DdeI | CTNAG | 1 cut(s) | 89 |
| FspBI | CTAG | 1 cut(s) | 11 |
| HinfI | GANTC | 2 cut(s) | 4, 103 |
| Hpy166II | GTNNAC | 1 cut(s) | 70 |
| Hpy188I | TCNGA | 1 cut(s) | 163 |
| Hpy188III | TCNNGA | 2 cut(s) | 11, 107 |
| Hpy8I | GTNNAC | 1 cut(s) | 70 |
| Hpy99I | CGWCG | 1 cut(s) | 124 |
| HpyF3I | CTNAG | 1 cut(s) | 89 |
| LmnI | GCTCC | 1 cut(s) | 25 |
| LweI | GCATC | 1 cut(s) | 51 |
| MaeI | CTAG | 1 cut(s) | 11 |
| MboII | GAAGA | 1 cut(s) | 145 |
| MlyI | GAGTC | 1 cut(s) | 112 |
| MmeI | TCCRAC | 1 cut(s) | 74 |
| MnlI | CCTC | 2 cut(s) | 33, 92 |
| NlaIV | GGNNCC | 1 cut(s) | 117 |
| NmeAIII | GCCGAG | 1 cut(s) | 18 |
| PleI | GAGTC | 1 cut(s) | 111 |
| PpsI | GAGTC | 1 cut(s) | 111 |
| PspN4I | GGNNCC | 1 cut(s) | 117 |
| SchI | GAGTC | 1 cut(s) | 112 |
| SfaNI | GCATC | 1 cut(s) | 51 |
| SgeI | CNNG | 9 cut(s) | 18, 23, 29, 36, 50, 95, 114, 119, 160 |
| SspI | AATATT | 1 cut(s) | 144 |
| SspMI | CTAG | 1 cut(s) | 11 |
| TaqI | TCGA | 2 cut(s) | 25, 106 |
| TspDTI | ATGAA | 1 cut(s) | 153 |
| TspGWI | ACGGA | 1 cut(s) | 49 |
| XbaI | TCTAGA | 1 cut(s) | 10 |
| XspI | CTAG | 1 cut(s) | 11 |