Rh2CG514700

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001054
Physical Location & Seq
Forward (+)
68869835 .. 68871453
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG514700.1

Sequence Viewer

Length: 1455 bp
ATGGCCAATCAACACCAAGAATCAAAGCGCCCTTCGTGGTTCACGCCCAAGAGGCTACTCGTCATATTCTGTGTGATCAACTTGATAAACTATGTCGACCGAGGAGCAATAGCAAGCAACGGCGTCAATGGAAACCTCGAAACTTGCAACAATGGCAAATGCACACCTGCTACGGGCATACAAGGCGATTTTAAGTTGAACAACTTTAAAGATGGCTGGTTATCTTCTGCTTTCATGGTTGGACTTCTCGTTTCTTCTCCAATATTTGCATCACTAGCCAAGGGTGTGAATCCGTTTCGGCTCATCGGAGTTGGACTGTCTGTCTGGACCTTGGCTGTAACTGGCTGTGGTTTCTCATTCAACTTCTGGTCCATCATTATTTGCCGCATGCTGGTTGGTGTTGGTGAGGCTTCATTTATTAGTCTCGCCGCCCCATTCATCGATGATAATGCCCCACCTCCTCAGAAAACTGTATGGCTTTCGATATTCTACATGTGCATACCATCTGGATATGCACTTGGTTATGTTTATGGTGGCTTGGTGGGCACTAGGCTAAGTTGGCGTGCTGCATTTTGGGGGGAGGCAATTCTGATGCTTCCCTTTGTGATTCTCGGGTTTGTTGTCAAGCCTTTGCAGATGAGAGGTTTTAAGCATGATGAATCAAAAGTTGCTGTGTCACAAGTTCAAGGTTCGGGTGCTTCCGAGATTAAAGTTGCAACGAAAATAAAGAAGCATCTTTCGAGGTTTATGAAAGATATGAAGGAGCTTTTGGTCGATAAGGTGTACATTGTTAATGTTCTTGGTTATGTAGCATACACCTTTGTAATTGGTGCCTACTCATATTGGGGACCCAAGGCTGGTTACAATATATATCATATGAATAATGCAGATATGATGTTTGGAGGTGTTACAATTGTGTGCGGAGTACTTGGCACAGTAGCAGGAGGCTTTGTTCTGGATTATATGTCTAACACTATCTCCAATGCTTTCAAGCTTCTTGCAACCACAACGTTTATTGGGGCTATATTATGCTTTGGTGCCTTTTGCTTGAAGAACACGTACGGTTTCCTAGCTCTTTTTGCAGTTGGACAACTACTTGTCTTTGCAATTCAGGGTCCCGTAAATTATGTTTGTCTGCATTGTGTGAAACCTAGTTTAAGGCCACTGTCTATGGCCATTTCTACTGTTGCAATTCACATCTTTGGAGATGTGCCTTCCTCGCCTCTCATCGGAGTTCTCCAGGATCACATCAAGAATTGGAGGTCGACTGCTCTTATTCTAACATCTATTTTTGTTCCAGCCGCTGCCATATGGTTTATAGGAATTTTTCTGCACAGCGTCGATAGGTACAGCGAAGAAAGTGAGAACCAGATATCCACAACTCAGGTTGAAGAAAAGAAGACAGAATCTACTGATTCTGTTGCCGAGCCTGTCACATATTCAACTCTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

484

Amino Acids

52.8

Weight (kDa)

8.83

Isoelectric Point (pI)

28.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 59 - 399 1.1e-31 Major Facilitator Superfamily
OATP PF03137 124 - 349 2e-21 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 348 - 416 6e-06 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 175
Acc36I ACCTGC 1 cut(s) 175
AccB1I GGYRCC 2 cut(s) 830, 1037
AccI GTMKAC 2 cut(s) 96, 1265
AciI CCGC 4 cut(s) 385, 429, 921, 1302
AclI AACGTT 1 cut(s) 1010
AclWI GGATC 1 cut(s) 1251
AcoI YGGCCR 2 cut(s) 3, 1173
AcsI RAATTY 1 cut(s) 1323
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 4 cut(s) 785, 927, 1061, 1349
AfiI CCNNNNNNNGG 4 cut(s) 173, 391, 857, 1229
AflIII ACRYGT 2 cut(s) 492, 1056
AgsI TTSAA 7 cut(s) 199, 361, 686, 991, 1051, 1391, 1443
AhdI GACNNNNNGTC 1 cut(s) 320
AjnI CCWGG 1 cut(s) 1239
AjuI GAANNNNNNNTTGG 2 cut(s) 752, 784
AloI GAACNNNNNNTCC 4 cut(s) 936, 968, 1358, 1390
AluBI AGCT 3 cut(s) 766, 994, 1073
AluI AGCT 3 cut(s) 766, 994, 1073
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 1 cut(s) 1251
AlwNI CAGNNNCTG 1 cut(s) 1304
Ama87I CYCGRG 1 cut(s) 611
AoxI GGCC 3 cut(s) 3, 1160, 1173
ApeKI GCWGC 2 cut(s) 566, 1304
ApoI RAATTY 1 cut(s) 1323
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 4 cut(s) 327, 369, 848, 1115
AsuHPI GGTGA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 611
AvaII GGWCC 4 cut(s) 327, 369, 848, 1115
BaeGI GKGCMC 1 cut(s) 548
BalI TGGCCA 2 cut(s) 5, 1175
BanI GGYRCC 2 cut(s) 830, 1037
BarI GAAGNNNNNNTAC 2 cut(s) 1043, 1075
BbsI GAAGAC 1 cut(s) 1406
BbvI GCAGC 2 cut(s) 553, 1291
BccI CCATC 3 cut(s) 206, 380, 511
BceAI ACGGC 1 cut(s) 136
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciT130I CCWGG 1 cut(s) 1241
BclI TGATCA 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 5 cut(s) 275, 549, 1070, 1152, 1453
BfoI RGCGCY 1 cut(s) 31
BfuAI ACCTGC 1 cut(s) 175
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 5 cut(s) 385, 429, 567, 1302, 1305
BlsI GCNGC 5 cut(s) 386, 430, 568, 1303, 1306
BmcAI AGTACT 1 cut(s) 927
Bme1390I CCNGG 1 cut(s) 1241
Bme18I GGWCC 4 cut(s) 327, 369, 848, 1115
BmeRI GACNNNNNGTC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 611
BmgT120I GGNCC 4 cut(s) 327, 369, 848, 1115
BmiI GGNNCC 6 cut(s) 832, 849, 850, 1039, 1116, 1117
BmrFI CCNGG 1 cut(s) 1241
BmsI GCATC 3 cut(s) 278, 582, 742
BpiI GAAGAC 1 cut(s) 1406
BpmI CTGGAG 1 cut(s) 1223
Bsa29I ATCGAT 1 cut(s) 441
BsaAI YACGTR 1 cut(s) 1059
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 4 cut(s) 100, 279, 330, 852
BsaXI ACNNNNNCTCC 4 cut(s) 936, 962, 966, 992
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 391, 857, 1229
Bse1I ACTGG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 1241
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 4 cut(s) 100, 279, 330, 852
BseLI CCNNNNNNNGG 4 cut(s) 173, 391, 857, 1229
BseMII CTCAG 2 cut(s) 476, 1397
BseNI ACTGG 1 cut(s) 346
BseRI GAGGAG 2 cut(s) 117, 450
BseSI GKGCMC 1 cut(s) 548
BseXI GCAGC 2 cut(s) 553, 1291
BsgI GTGCAG 1 cut(s) 1316
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 3 cut(s) 5, 1162, 1175
BshNI GGYRCC 2 cut(s) 830, 1037
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 1 cut(s) 100
BsiHKCI CYCGRG 1 cut(s) 611
BsiWI CGTACG 1 cut(s) 1059
BslFI GGGAC 2 cut(s) 861, 1101
BslI CCNNNNNNNGG 4 cut(s) 173, 391, 857, 1229
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 2 cut(s) 861, 1101
BsnI GGCC 3 cut(s) 5, 1162, 1175
BsoBI CYCGRG 1 cut(s) 611
Bsp1286I GDGCHC 1 cut(s) 548
Bsp1407I TGTACA 1 cut(s) 783
Bsp143I GATC 2 cut(s) 75, 1243
BspACI CCGC 4 cut(s) 385, 429, 921, 1302
BspANI GGCC 3 cut(s) 5, 1162, 1175
BspCNI CTCAG 2 cut(s) 475, 1396
BspDI ATCGAT 1 cut(s) 441
BspLI GGNNCC 6 cut(s) 832, 849, 850, 1039, 1116, 1117
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 1 cut(s) 1251
BspT107I GGYRCC 2 cut(s) 830, 1037
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 1 cut(s) 346
BssECI CCNNGG 4 cut(s) 100, 279, 330, 852
BssMI GATC 2 cut(s) 75, 1243
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 3 cut(s) 279, 330, 852
Bst2UI CCWGG 1 cut(s) 1241
Bst4CI ACNGT 6 cut(s) 318, 472, 937, 1064, 1167, 1186
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 783
BstBAI YACGTR 1 cut(s) 1059
BstC8I GCNNGC 3 cut(s) 115, 389, 564
BstDEI CTNAG 3 cut(s) 462, 554, 1383
BstH2I RGCGCY 1 cut(s) 31
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 2 cut(s) 78, 1246
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 2 cut(s) 75, 1243
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 8 cut(s) 52, 153, 183, 275, 543, 559, 1079, 1219
BstNI CCWGG 1 cut(s) 1241
BstNSI RCATGY 2 cut(s) 391, 496
BstSCI CCNGG 1 cut(s) 1239
BstSLI GKGCMC 1 cut(s) 548
BstV1I GCAGC 2 cut(s) 553, 1291
BstV2I GAAGAC 1 cut(s) 1406
Bsu15I ATCGAT 1 cut(s) 441
BsuRI GGCC 3 cut(s) 5, 1162, 1175
BsuTUI ATCGAT 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 1163
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 3 cut(s) 115, 389, 564
CaiI CAGNNNCTG 1 cut(s) 1304
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 4 cut(s) 327, 369, 848, 1115
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 2 cut(s) 112, 1327
Csp6I GTAC 4 cut(s) 784, 926, 1060, 1348
CviAII CATG 4 cut(s) 235, 388, 493, 653
CviQI GTAC 4 cut(s) 784, 926, 1060, 1348
DdeI CTNAG 3 cut(s) 462, 554, 1383
DpnI GATC 2 cut(s) 77, 1245
DpnII GATC 2 cut(s) 75, 1243
DraI TTTAAA 1 cut(s) 208
DriI GACNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 2 cut(s) 3, 1173
Eam1105I GACNNNNNGTC 1 cut(s) 320
Eco130I CCWWGG 3 cut(s) 279, 330, 852
Eco32I GATATC 1 cut(s) 1374
Eco47I GGWCC 4 cut(s) 327, 369, 848, 1115
Eco88I CYCGRG 1 cut(s) 611
EcoO109I RGGNCCY 2 cut(s) 848, 1115
EcoRII CCWGG 1 cut(s) 1239
EcoRV GATATC 1 cut(s) 1374
EcoT14I CCWWGG 3 cut(s) 279, 330, 852
ErhI CCWWGG 3 cut(s) 279, 330, 852
FaeI CATG 4 cut(s) 238, 391, 496, 656
FaqI GGGAC 2 cut(s) 861, 1101
FatI CATG 4 cut(s) 234, 387, 492, 652
FauNDI CATATG 2 cut(s) 876, 1310
FbaI TGATCA 1 cut(s) 75
FblI GTMKAC 2 cut(s) 96, 1265
Fnu4HI GCNGC 5 cut(s) 385, 429, 567, 1302, 1305
Fsp4HI GCNGC 5 cut(s) 385, 429, 567, 1302, 1305
FspBI CTAG 5 cut(s) 275, 549, 1070, 1152, 1453
GlaI GCGC 1 cut(s) 29
GluI GCNGC 5 cut(s) 385, 429, 567, 1302, 1305
GsuI CTGGAG 1 cut(s) 1223
HaeII RGCGCY 1 cut(s) 31
HaeIII GGCC 3 cut(s) 5, 1162, 1175
HgaI GACGC 2 cut(s) 112, 1327
HhaI GCGC 1 cut(s) 30
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 4 cut(s) 238, 391, 496, 656
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HincII GTYRAC 2 cut(s) 97, 1266
HindII GTYRAC 2 cut(s) 97, 1266
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 6 cut(s) 20, 289, 607, 659, 1406, 1415
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 4 cut(s) 42, 97, 784, 1266
Hpy188I TCNGA 5 cut(s) 308, 465, 591, 703, 1232
Hpy188III TCNNGA 4 cut(s) 325, 507, 956, 1252
Hpy8I GTNNAC 4 cut(s) 42, 97, 784, 1266
Hpy99I CGWCG 1 cut(s) 1343
HpyAV CCTTC 3 cut(s) 42, 754, 1224
HpyCH4III ACNGT 6 cut(s) 318, 472, 937, 1064, 1167, 1186
HpyCH4IV ACGT 2 cut(s) 1010, 1058
HpyF10VI GCNNNNNNNGC 8 cut(s) 52, 153, 183, 275, 543, 559, 1079, 1219
HpyF3I CTNAG 3 cut(s) 462, 554, 1383
HpySE526I ACGT 2 cut(s) 1010, 1058
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 4 cut(s) 238, 391, 496, 656
HspAI GCGC 1 cut(s) 28
KflI GGGWCCC 2 cut(s) 848, 1115
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 2 cut(s) 75, 1243
LmnI GCTCC 2 cut(s) 104, 763
Lsp1109I GCAGC 2 cut(s) 553, 1291
LweI GCATC 3 cut(s) 278, 582, 742
MaeI CTAG 5 cut(s) 275, 549, 1070, 1152, 1453
MaeII ACGT 2 cut(s) 1010, 1058
MaeIII GTNAC 5 cut(s) 337, 675, 860, 907, 1432
MalI GATC 2 cut(s) 77, 1245
MboI GATC 2 cut(s) 75, 1243
MboII GAAGA 6 cut(s) 216, 246, 1063, 1367, 1403, 1411
MfeI CAATTG 1 cut(s) 912
MhlI GDGCHC 1 cut(s) 548
MlsI TGGCCA 2 cut(s) 5, 1175
MluCI AATT 8 cut(s) 585, 825, 912, 1107, 1123, 1191, 1255, 1323
MluNI TGGCCA 2 cut(s) 5, 1175
MmeI TCCRAC 3 cut(s) 220, 292, 1066
Mox20I TGGCCA 2 cut(s) 5, 1175
MscI TGGCCA 2 cut(s) 5, 1175
MseI TTAA 6 cut(s) 192, 207, 648, 708, 792, 1157
Msp20I TGGCCA 2 cut(s) 5, 1175
MspA1I CMGCKG 1 cut(s) 1304
MspR9I CCNGG 1 cut(s) 1241
MunI CAATTG 1 cut(s) 912
MvaI CCWGG 1 cut(s) 1241
MwoI GCNNNNNNNGC 8 cut(s) 52, 153, 183, 275, 543, 559, 1079, 1219
NdeI CATATG 2 cut(s) 876, 1310
NdeII GATC 2 cut(s) 75, 1243
NlaIII CATG 4 cut(s) 238, 391, 496, 656
NlaIV GGNNCC 6 cut(s) 832, 849, 850, 1039, 1116, 1117
NmeAIII GCCGAG 1 cut(s) 1450
NmuCI GTSAC 2 cut(s) 675, 1432
NspI RCATGY 2 cut(s) 391, 496
PaeI GCATGC 1 cut(s) 391
PaqCI CACCTGC 1 cut(s) 175
PciI ACATGT 1 cut(s) 492
PfeI GAWTC 6 cut(s) 20, 289, 607, 659, 1406, 1415
Pfl23II CGTACG 1 cut(s) 1059
PfoI TCCNGGA 1 cut(s) 1239
PkrI GCNGC 5 cut(s) 386, 430, 568, 1303, 1306
Ppu21I YACGTR 1 cut(s) 1059
PpuMI RGGWCCY 2 cut(s) 848, 1115
PscI ACATGT 1 cut(s) 492
Psp1406I AACGTT 1 cut(s) 1010
Psp5II RGGWCCY 2 cut(s) 848, 1115
Psp6I CCWGG 1 cut(s) 1239
PspGI CCWGG 1 cut(s) 1239
PspLI CGTACG 1 cut(s) 1059
PspN4I GGNNCC 6 cut(s) 832, 849, 850, 1039, 1116, 1117
PspPI GGNCC 4 cut(s) 327, 369, 848, 1115
PspPPI RGGWCCY 2 cut(s) 848, 1115
PstNI CAGNNNCTG 1 cut(s) 1304
RsaI GTAC 4 cut(s) 785, 927, 1061, 1349
RsaNI GTAC 4 cut(s) 784, 926, 1060, 1348
SalI GTCGAC 2 cut(s) 95, 1264
SaqAI TTAA 6 cut(s) 192, 207, 648, 708, 792, 1157
SatI GCNGC 5 cut(s) 385, 429, 567, 1302, 1305
Sau3AI GATC 2 cut(s) 75, 1243
Sau96I GGNCC 4 cut(s) 327, 369, 848, 1115
ScaI AGTACT 1 cut(s) 927
ScrFI CCNGG 1 cut(s) 1241
SduI GDGCHC 1 cut(s) 548
SfaNI GCATC 3 cut(s) 278, 582, 742
SinI GGWCC 4 cut(s) 327, 369, 848, 1115
SphI GCATGC 1 cut(s) 391
Sse9I AATT 8 cut(s) 585, 825, 912, 1107, 1123, 1191, 1255, 1323
SsiI CCGC 4 cut(s) 385, 429, 921, 1302
SspI AATATT 1 cut(s) 264
SspMI CTAG 5 cut(s) 275, 549, 1070, 1152, 1453
StyD4I CCNGG 1 cut(s) 1239
StyI CCWWGG 3 cut(s) 279, 330, 852
TaaI ACNGT 6 cut(s) 318, 472, 937, 1064, 1167, 1186
TaiI ACGT 2 cut(s) 1013, 1061
TaqI TCGA 8 cut(s) 96, 138, 441, 482, 740, 774, 1265, 1341
TaqII GACCGA 1 cut(s) 114
TasI AATT 8 cut(s) 585, 825, 912, 1107, 1123, 1191, 1255, 1323
TatI WGTACW 2 cut(s) 783, 925
TauI GCSGC 3 cut(s) 387, 431, 1304
TfiI GAWTC 6 cut(s) 20, 289, 607, 659, 1406, 1415
Tru1I TTAA 6 cut(s) 192, 207, 648, 708, 792, 1157
Tru9I TTAA 6 cut(s) 192, 207, 648, 708, 792, 1157
TscAI CASTG 1 cut(s) 1170
TseFI GTSAC 2 cut(s) 675, 1432
TseI GCWGC 2 cut(s) 566, 1304
Tsp45I GTSAC 2 cut(s) 675, 1432
TspDTI ATGAA 7 cut(s) 223, 402, 427, 672, 764, 773, 893
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 1170
VpaK11BI GGWCC 4 cut(s) 327, 369, 848, 1115
XapI RAATTY 1 cut(s) 1323
XceI RCATGY 2 cut(s) 391, 496
XmiI GTMKAC 2 cut(s) 96, 1265
XspI CTAG 5 cut(s) 275, 549, 1070, 1152, 1453
ZrmI AGTACT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.