FvH4_1g22042

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001114
Physical Location & Seq
Reverse (-)
13991530 .. 13991910
381 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22042.t1

Sequence Viewer

Length: 381 bp
ATGGGAAAATGTATTCTAAGGACACTAGGTTATACGGATCCAGGGTATCTTTGCTCAGGCCAACTGACAGACAAGAGTGATGTTTATAGCTTCGGAGTCGTTTTGGTAGAGATTTTGAGCCAGGAAAAGCCTATTTCATTTGACAGACCAGAAAGTCAAAGAATCATTGCTACTCATTTTGTTTCATCCATTGAATTAGAAGATGGGTTCTTTCGTGTGGTTGATCCACAACATGTAAACAAGGGAAACCATGAACAAGTTAGAGCAGTTGCAGAGCTTGCCAAGAGATGCCTCCATATGAACAGTGCAGAAAGGCCCTTAATGAAAGAAGTCGCCGAAGAGTTGAAGAGGCTGTCTGGCATGTGTCCTACAATAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

127

Amino Acids

14.15

Weight (kDa)

6.07

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 7 - 113 1.9e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 14 - 115 8.6e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 153
AclWI GGATC 3 cut(s) 32, 45, 218
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 2 cut(s) 194, 346
AjnI CCWGG 2 cut(s) 40, 120
AluBI AGCT 2 cut(s) 90, 277
AluI AGCT 2 cut(s) 90, 277
AlwI GGATC 3 cut(s) 32, 45, 218
AoxI GGCC 2 cut(s) 58, 314
AspS9I GGNCC 1 cut(s) 315
BamHI GGATCC 1 cut(s) 37
BccI CCATC 1 cut(s) 197
BciT130I CCWGG 2 cut(s) 42, 122
BfaI CTAG 1 cut(s) 26
Bme1390I CCNGG 2 cut(s) 42, 122
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 42, 122
BmsI GCATC 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 41
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 2 cut(s) 42, 122
BseDI CCNNGG 1 cut(s) 41
BseGI GGATG 1 cut(s) 185
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 69
BsgI GTGCAG 1 cut(s) 327
BshFI GGCC 2 cut(s) 60, 316
BsnI GGCC 2 cut(s) 60, 316
Bsp143I GATC 2 cut(s) 37, 223
BspANI GGCC 2 cut(s) 60, 316
BspCNI CTCAG 1 cut(s) 68
BspLI GGNNCC 1 cut(s) 39
BspPI GGATC 3 cut(s) 32, 45, 218
BsrDI GCAATG 1 cut(s) 165
BssECI CCNNGG 1 cut(s) 41
BssMI GATC 2 cut(s) 37, 223
Bst2UI CCWGG 2 cut(s) 42, 122
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 2 cut(s) 333, 341
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 2 cut(s) 17, 55
BstF5I GGATG 1 cut(s) 185
BstKTI GATC 2 cut(s) 40, 226
BstMBI GATC 2 cut(s) 37, 223
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNI CCWGG 2 cut(s) 42, 122
BstNSI RCATGY 2 cut(s) 236, 364
BstSCI CCNGG 2 cut(s) 40, 120
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 60, 316
BtsCI GGATG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 279
Cfr13I GGNCC 1 cut(s) 315
CviAII CATG 3 cut(s) 233, 251, 361
CviJI RGCY 7 cut(s) 60, 90, 120, 130, 277, 316, 352
CviKI_1 RGCY 7 cut(s) 60, 90, 120, 130, 277, 316, 352
DdeI CTNAG 2 cut(s) 17, 55
DpnI GATC 2 cut(s) 39, 225
DpnII GATC 2 cut(s) 37, 223
DrdI GACNNNNNNGTC 1 cut(s) 153
DseDI GACNNNNNNGTC 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 333, 341
EarI CTCTTC 2 cut(s) 333, 341
EcoO109I RGGNCCY 1 cut(s) 315
EcoRII CCWGG 2 cut(s) 40, 120
FaeI CATG 3 cut(s) 236, 254, 364
FaiI YATR 7 cut(s) 33, 87, 234, 252, 297, 299, 362
FatI CATG 3 cut(s) 232, 250, 360
FauNDI CATATG 1 cut(s) 297
FokI GGATG 1 cut(s) 172
FspBI CTAG 1 cut(s) 26
HaeIII GGCC 2 cut(s) 60, 316
Hin1II CATG 3 cut(s) 236, 254, 364
HinfI GANTC 2 cut(s) 96, 162
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 2 cut(s) 95, 380
Hpy8I GTNNAC 1 cut(s) 238
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4V TGCA 2 cut(s) 272, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 2 cut(s) 17, 55
Hsp92II CATG 3 cut(s) 236, 254, 364
Kzo9I GATC 2 cut(s) 37, 223
LpnPI CCDG 7 cut(s) 27, 42, 54, 107, 134, 162, 342
LweI GCATC 1 cut(s) 278
MaeI CTAG 1 cut(s) 26
MalI GATC 2 cut(s) 39, 225
MboI GATC 2 cut(s) 37, 223
MboII GAAGA 3 cut(s) 212, 350, 358
MflI RGATCY 1 cut(s) 37
MluCI AATT 1 cut(s) 194
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 2 cut(s) 302, 342
MseI TTAA 1 cut(s) 320
MspR9I CCNGG 2 cut(s) 42, 122
MvaI CCWGG 2 cut(s) 42, 122
MwoI GCNNNNNNNGC 1 cut(s) 278
NdeI CATATG 1 cut(s) 297
NdeII GATC 2 cut(s) 37, 223
NlaIII CATG 3 cut(s) 236, 254, 364
NlaIV GGNNCC 1 cut(s) 39
NspI RCATGY 2 cut(s) 236, 364
PciI ACATGT 1 cut(s) 232
PfeI GAWTC 1 cut(s) 162
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PscI ACATGT 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 40, 120
PspGI CCWGG 2 cut(s) 40, 120
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 315
PsuI RGATCY 1 cut(s) 37
SaqAI TTAA 1 cut(s) 320
Sau3AI GATC 2 cut(s) 37, 223
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 2 cut(s) 42, 122
SetI ASST 3 cut(s) 31, 92, 279
SfaNI GCATC 1 cut(s) 278
Sse9I AATT 1 cut(s) 194
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 40, 120
TaaI ACNGT 1 cut(s) 305
TasI AATT 1 cut(s) 194
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TscAI CASTG 1 cut(s) 310
TspDTI ATGAA 5 cut(s) 126, 174, 267, 314, 338
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 1 cut(s) 310
XceI RCATGY 2 cut(s) 236, 364
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.