Rmu_sc0005139.1_g000022

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005139.1
OG0001114
Physical Location & Seq
Reverse (-)
97255 .. 104087
6833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005139.1_g000022.1.cds

Sequence Viewer

Length: 1353 bp
atgcgttgtcgaacttccgatactactgggttttgttacaaacaatccagtggtaatagaggtgctcatctttacaccggcattggcagtacccattattatcttgattcttttcctcctggacaccaagctgggacttctcgtgatggtgatattccggaaggagttatctttaaagtaacaagttttgagcagttgaagcaaccatcattcaactcgaaatcatttgtcacatacatacaaccggtgccctttgccgtgtttgctcgcacaaaacgggaattggagtaccaaccaaggaaagatggtaaagtttggatctcacaccgtgtaaagcttactctagctagtattaagaagcaaaactctgttatgctcaaatccgaattcttcaagaaaaatggacggttattgttaaagcaacatattgatgcttcacaatatggaagcacaatgatatttacagccgaggaactacagatggctactgaattttacaagaagaaacacttctttggtccaggaggagacggcggaagtactgtctatgacaaaggatttttagtggaggagccccaagtggagcattttatgaatgatattatcgctcttaccaaaattggccatcgaaatttgcgggaaaagtcattgccatgggatacccttttaaagatagcctcagaatccgcagctgctcttgcacacctacattcttcttctacagcagatcactcgcaaccagtgattcatggaaatgtcaagtcttccaaaatattattgacggattgtttcatggccaaagtcactgagtatcttgttacatgccaattgacagataaaagtgatgtgtatagctttggagtcgttttgttggaaattttaactggggagatgtcggtggtgcaccatccaagtccagcctcgtcgtttctaagtccatatatggtaagaaacagcagcagtgtgacaccagtatcagctcgacttactacatccatggtgatggtatggccaaccttgaattctagttcagagaaggaatcccttggatctaagaaatgggaaaagtacttcattgccttaggatgcccttctaaagatatcctaacagattcagcagcaagtacttggaatggcgaagagaggcggctcggcagggaaggaatgggatgtcatcaagtcaggagaggtaccgatcgagccattaccaatgcttactacagaggtgctgttggtgctctattggtctatgacatagccaaaaggcaaacatttgacaatgtgacgaggtgcatggctccgcgaactcagagaccatgcaaactcaaatattgtcataatgatggccggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

450

Amino Acids

50.35

Weight (kDa)

9.33

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1190
AccB1I GGYRCC 2 cut(s) 247, 1190
AccII CGCG 1 cut(s) 1303
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 5 cut(s) 534, 637, 687, 1147, 1301
AclWI GGATC 2 cut(s) 326, 1057
AcoI YGGCCR 4 cut(s) 622, 795, 1010, 1345
AcsI RAATTY 5 cut(s) 386, 491, 631, 876, 1021
AdeI CACNNNGTG 1 cut(s) 329
AfaI GTAC 6 cut(s) 91, 290, 541, 1070, 1126, 1192
AgeI ACCGGT 1 cut(s) 244
AgsI TTSAA 4 cut(s) 199, 214, 394, 1021
AjnI CCWGG 2 cut(s) 118, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 1006, 1038
AluBI AGCT 6 cut(s) 131, 337, 347, 692, 855, 980
AluI AGCT 6 cut(s) 131, 337, 347, 692, 855, 980
Alw21I GWGCWC 3 cut(s) 67, 906, 1240
Alw26I GTCTC 2 cut(s) 522, 1306
Alw44I GTGCAC 1 cut(s) 902
AlwI GGATC 2 cut(s) 326, 1057
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 4 cut(s) 622, 795, 1010, 1345
ApaLI GTGCAC 1 cut(s) 902
ApeKI GCWGC 4 cut(s) 689, 692, 957, 1118
ApoI RAATTY 5 cut(s) 386, 491, 631, 876, 1021
AsiGI ACCGGT 1 cut(s) 244
Asp700I GAANNNNTTC 1 cut(s) 509
Asp718I GGTACC 1 cut(s) 1190
AspS9I GGNCC 1 cut(s) 518
AsuHPI GGTGA 2 cut(s) 161, 1012
AvaII GGWCC 1 cut(s) 518
AxyI CCTNAGG 1 cut(s) 1081
BaeGI GKGCMC 2 cut(s) 252, 906
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BalI TGGCCA 3 cut(s) 624, 797, 1012
BanI GGYRCC 2 cut(s) 247, 1190
BanII GRGCYC 1 cut(s) 576
BauI CACGAG 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 3 cut(s) 67, 906, 1240
BbvI GCAGC 4 cut(s) 679, 701, 969, 1130
BccI CCATC 8 cut(s) 140, 214, 299, 475, 633, 915, 997, 1337
BceAI ACGGC 2 cut(s) 242, 547
BcgI CGANNNNNNTGC 2 cut(s) 714, 748
BciT130I CCWGG 2 cut(s) 120, 522
BciVI GTATCC 1 cut(s) 652
BcoDI GTCTC 2 cut(s) 522, 1306
BfaI CTAG 3 cut(s) 344, 348, 1026
BfmI CTRYAG 3 cut(s) 476, 720, 1219
BfuI GTATCC 1 cut(s) 652
BisI GCNGC 5 cut(s) 690, 693, 958, 1119, 1148
BlsI GCNGC 5 cut(s) 691, 694, 959, 1120, 1149
BmcAI AGTACT 3 cut(s) 541, 1070, 1126
Bme1390I CCNGG 2 cut(s) 120, 522
Bme18I GGWCC 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 518
BmiI GGNNCC 4 cut(s) 249, 573, 1192, 1299
BmrFI CCNGG 2 cut(s) 120, 522
BmrI ACTGGG 2 cut(s) 36, 894
BmsI GCATC 2 cut(s) 421, 1076
BmuI ACTGGG 2 cut(s) 36, 894
BpiI GAAGAC 1 cut(s) 756
BsaI GGTCTC 1 cut(s) 1306
BsaJI CCNNGG 5 cut(s) 296, 468, 653, 996, 1045
BsaWI WCCGGW 2 cut(s) 157, 244
Bse118I RCCGGY 3 cut(s) 77, 244, 1347
Bse1I ACTGG 5 cut(s) 31, 48, 740, 889, 971
Bse21I CCTNAGG 1 cut(s) 1081
Bse3DI GCAATG 2 cut(s) 647, 1074
BseAI TCCGGA 1 cut(s) 157
BseBI CCWGG 2 cut(s) 120, 522
BseDI CCNNGG 5 cut(s) 296, 468, 653, 996, 1045
BseGI GGATG 4 cut(s) 907, 992, 1091, 1175
BseMI GCAATG 2 cut(s) 647, 1074
BseMII CTCAG 3 cut(s) 693, 798, 1322
BseNI ACTGG 5 cut(s) 31, 48, 740, 889, 971
BseRI GAGGAG 2 cut(s) 540, 584
BseSI GKGCMC 2 cut(s) 252, 906
BseXI GCAGC 4 cut(s) 679, 701, 969, 1130
BseYI CCCAGC 1 cut(s) 131
Bsh1236I CGCG 1 cut(s) 1303
Bsh1285I CGRYCG 1 cut(s) 1198
BshFI GGCC 4 cut(s) 624, 797, 1012, 1347
BshNI GGYRCC 2 cut(s) 247, 1190
BshTI ACCGGT 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 1198
BsiHKAI GWGCWC 3 cut(s) 67, 906, 1240
BsiSI CCGG 4 cut(s) 78, 158, 245, 1348
BslFI GGGAC 1 cut(s) 148
BsmAI GTCTC 2 cut(s) 522, 1306
BsmBI CGTCTC 1 cut(s) 522
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 4 cut(s) 624, 797, 1012, 1347
Bso31I GGTCTC 1 cut(s) 1306
Bsp1286I GDGCHC 5 cut(s) 67, 252, 576, 906, 1240
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 4 cut(s) 318, 727, 1049, 1195
Bsp19I CCATGG 2 cut(s) 653, 996
BspACI CCGC 5 cut(s) 534, 637, 687, 1147, 1301
BspANI GGCC 4 cut(s) 624, 797, 1012, 1347
BspCNI CTCAG 3 cut(s) 692, 799, 1321
BspEI TCCGGA 1 cut(s) 157
BspFNI CGCG 1 cut(s) 1303
BspLI GGNNCC 4 cut(s) 249, 573, 1192, 1299
BspPI GGATC 2 cut(s) 326, 1057
BspT107I GGYRCC 2 cut(s) 247, 1190
BspTNI GGTCTC 1 cut(s) 1306
BsrDI GCAATG 2 cut(s) 647, 1074
BsrFI RCCGGY 3 cut(s) 77, 244, 1347
BsrI ACTGG 5 cut(s) 31, 48, 740, 889, 971
BssAI RCCGGY 3 cut(s) 77, 244, 1347
BssECI CCNNGG 5 cut(s) 296, 468, 653, 996, 1045
BssMI GATC 4 cut(s) 318, 727, 1049, 1195
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 4 cut(s) 296, 653, 996, 1045
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 120, 522
Bst4CI ACNGT 3 cut(s) 329, 408, 544
Bst6I CTCTTC 1 cut(s) 1134
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 6 cut(s) 679, 807, 932, 1053, 1081, 1308
BstDSI CCRYGG 2 cut(s) 653, 996
BstF5I GGATG 4 cut(s) 907, 992, 1091, 1175
BstFNI CGCG 1 cut(s) 1303
BstKTI GATC 4 cut(s) 321, 730, 1052, 1198
BstMAI GTCTC 2 cut(s) 522, 1306
BstMBI GATC 4 cut(s) 318, 727, 1049, 1195
BstMCI CGRYCG 1 cut(s) 1198
BstMWI GCNNNNNNNGC 4 cut(s) 199, 263, 698, 1235
BstNI CCWGG 2 cut(s) 120, 522
BstNSI RCATGY 1 cut(s) 825
BstSCI CCNGG 2 cut(s) 118, 520
BstSFI CTRYAG 3 cut(s) 476, 720, 1219
BstSLI GKGCMC 2 cut(s) 252, 906
BstUI CGCG 1 cut(s) 1303
BstV1I GCAGC 4 cut(s) 679, 701, 969, 1130
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 2 cut(s) 318, 1049
BstXI CCANNNNNNTGG 1 cut(s) 1003
BstYI RGATCY 2 cut(s) 318, 1049
Bsu36I CCTNAGG 1 cut(s) 1081
BsuI GTATCC 1 cut(s) 652
BsuRI GGCC 4 cut(s) 624, 797, 1012, 1347
BtgI CCRYGG 2 cut(s) 653, 996
BtsCI GGATG 4 cut(s) 907, 992, 1091, 1175
BtsI GCAGTG 1 cut(s) 967
BtsIMutI CAGTG 4 cut(s) 55, 747, 804, 967
Cac8I GCNNGC 1 cut(s) 268
Cfr10I RCCGGY 3 cut(s) 77, 244, 1347
Cfr13I GGNCC 1 cut(s) 518
Csp6I GTAC 6 cut(s) 90, 289, 540, 1069, 1125, 1191
CspAI ACCGGT 1 cut(s) 244
CviAII CATG 7 cut(s) 654, 749, 793, 822, 997, 1294, 1317
CviQI GTAC 6 cut(s) 90, 289, 540, 1069, 1125, 1191
DdeI CTNAG 6 cut(s) 679, 807, 932, 1053, 1081, 1308
DpnI GATC 4 cut(s) 320, 729, 1051, 1197
DpnII GATC 4 cut(s) 318, 727, 1049, 1195
DraI TTTAAA 2 cut(s) 175, 669
DraIII CACNNNGTG 1 cut(s) 329
EaeI YGGCCR 4 cut(s) 622, 795, 1010, 1345
Eam1104I CTCTTC 1 cut(s) 1134
EarI CTCTTC 1 cut(s) 1134
EciI GGCGGA 1 cut(s) 549
Eco130I CCWWGG 4 cut(s) 296, 653, 996, 1045
Eco24I GRGCYC 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 1306
Eco32I GATATC 1 cut(s) 1102
Eco47I GGWCC 1 cut(s) 518
Eco81I CCTNAGG 1 cut(s) 1081
EcoRI GAATTC 2 cut(s) 386, 1021
EcoRII CCWGG 2 cut(s) 118, 520
EcoRV GATATC 1 cut(s) 1102
EcoT14I CCWWGG 4 cut(s) 296, 653, 996, 1045
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 4 cut(s) 296, 653, 996, 1045
Esp3I CGTCTC 1 cut(s) 522
FaeI CATG 7 cut(s) 657, 752, 796, 825, 1000, 1297, 1320
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 1 cut(s) 148
FatI CATG 7 cut(s) 653, 748, 792, 821, 996, 1293, 1316
FauI CCCGC 1 cut(s) 630
Fnu4HI GCNGC 5 cut(s) 690, 693, 958, 1119, 1148
FokI GGATG 4 cut(s) 894, 979, 1098, 1182
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 5 cut(s) 690, 693, 958, 1119, 1148
FspBI CTAG 3 cut(s) 344, 348, 1026
GluI GCNGC 5 cut(s) 690, 693, 958, 1119, 1148
GsaI CCCAGC 1 cut(s) 135
HaeIII GGCC 4 cut(s) 624, 797, 1012, 1347
HapII CCGG 4 cut(s) 78, 158, 245, 1348
Hin1II CATG 7 cut(s) 657, 752, 796, 825, 1000, 1297, 1320
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 6 cut(s) 107, 683, 745, 861, 1040, 1112
HpaII CCGG 4 cut(s) 78, 158, 245, 1348
HphI GGTGA 2 cut(s) 161, 1012
Hpy166II GTNNAC 1 cut(s) 904
Hpy188I TCNGA 5 cut(s) 19, 385, 682, 1033, 1311
Hpy188III TCNNGA 5 cut(s) 104, 143, 158, 394, 1183
Hpy8I GTNNAC 1 cut(s) 904
Hpy99I CGWCG 1 cut(s) 928
HpyAV CCTTC 4 cut(s) 155, 1030, 1101, 1154
HpyCH4III ACNGT 3 cut(s) 329, 408, 544
HpyCH4V TGCA 4 cut(s) 701, 904, 1293, 1320
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 263, 698, 1235
HpyF3I CTNAG 6 cut(s) 679, 807, 932, 1053, 1081, 1308
Hsp92II CATG 7 cut(s) 657, 752, 796, 825, 1000, 1297, 1320
Kpn2I TCCGGA 1 cut(s) 157
KpnI GGTACC 1 cut(s) 1194
Kzo9I GATC 4 cut(s) 318, 727, 1049, 1195
LmnI GCTCC 3 cut(s) 571, 583, 1303
Lsp1109I GCAGC 4 cut(s) 679, 701, 969, 1130
LweI GCATC 2 cut(s) 421, 1076
MaeI CTAG 3 cut(s) 344, 348, 1026
MaeIII GTNAC 7 cut(s) 35, 178, 229, 802, 817, 964, 1282
MalI GATC 4 cut(s) 320, 729, 1051, 1197
MboI GATC 4 cut(s) 318, 727, 1049, 1195
MboII GAAGA 6 cut(s) 382, 514, 705, 708, 756, 1151
MfeI CAATTG 1 cut(s) 827
MflI RGATCY 2 cut(s) 318, 1049
MhlI GDGCHC 5 cut(s) 67, 252, 576, 906, 1240
MlsI TGGCCA 3 cut(s) 624, 797, 1012
MluCI AATT 8 cut(s) 281, 386, 491, 618, 631, 827, 876, 1021
MluNI TGGCCA 3 cut(s) 624, 797, 1012
MlyI GAGTC 1 cut(s) 870
MmeI TCCRAC 1 cut(s) 852
Mox20I TGGCCA 3 cut(s) 624, 797, 1012
MroI TCCGGA 1 cut(s) 157
MroXI GAANNNNTTC 1 cut(s) 509
MscI TGGCCA 3 cut(s) 624, 797, 1012
MseI TTAA 5 cut(s) 174, 354, 416, 668, 881
MslI CAYNNNNRTG 5 cut(s) 429, 652, 753, 1001, 1341
Msp20I TGGCCA 3 cut(s) 624, 797, 1012
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 4 cut(s) 78, 158, 245, 1348
MspR9I CCNGG 2 cut(s) 120, 522
MunI CAATTG 1 cut(s) 827
MvaI CCWGG 2 cut(s) 120, 522
MvnI CGCG 1 cut(s) 1303
MwoI GCNNNNNNNGC 4 cut(s) 199, 263, 698, 1235
NcoI CCATGG 2 cut(s) 653, 996
NdeII GATC 4 cut(s) 318, 727, 1049, 1195
NlaIII CATG 7 cut(s) 657, 752, 796, 825, 1000, 1297, 1320
NlaIV GGNNCC 4 cut(s) 249, 573, 1192, 1299
NmeAIII GCCGAG 2 cut(s) 493, 1131
NmuCI GTSAC 4 cut(s) 229, 802, 964, 1282
NspI RCATGY 1 cut(s) 825
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 5 cut(s) 107, 683, 745, 1040, 1112
PfoI TCCNGGA 2 cut(s) 118, 520
PinAI ACCGGT 1 cut(s) 244
PkrI GCNGC 5 cut(s) 691, 694, 959, 1120, 1149
Ple19I CGATCG 1 cut(s) 1198
PleI GAGTC 1 cut(s) 869
PpsI GAGTC 1 cut(s) 869
Psp6I CCWGG 2 cut(s) 118, 520
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 2 cut(s) 118, 520
PspN4I GGNNCC 4 cut(s) 249, 573, 1192, 1299
PspPI GGNCC 1 cut(s) 518
PsrI GAACNNNNNNTAC 1 cut(s) 36
PsuI RGATCY 2 cut(s) 318, 1049
PvuI CGATCG 1 cut(s) 1198
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 6 cut(s) 91, 290, 541, 1070, 1126, 1192
RsaNI GTAC 6 cut(s) 90, 289, 540, 1069, 1125, 1191
RseI CAYNNNNRTG 5 cut(s) 429, 652, 753, 1001, 1341
SaqAI TTAA 5 cut(s) 174, 354, 416, 668, 881
SatI GCNGC 5 cut(s) 690, 693, 958, 1119, 1148
Sau3AI GATC 4 cut(s) 318, 727, 1049, 1195
Sau96I GGNCC 1 cut(s) 518
ScaI AGTACT 3 cut(s) 541, 1070, 1126
SchI GAGTC 1 cut(s) 870
ScrFI CCNGG 2 cut(s) 120, 522
SduI GDGCHC 5 cut(s) 67, 252, 576, 906, 1240
SfaNI GCATC 2 cut(s) 421, 1076
SfcI CTRYAG 3 cut(s) 476, 720, 1219
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 5 cut(s) 429, 652, 753, 1001, 1341
Sse9I AATT 8 cut(s) 281, 386, 491, 618, 631, 827, 876, 1021
SsiI CCGC 5 cut(s) 534, 637, 687, 1147, 1301
SspI AATATT 2 cut(s) 774, 1331
SspMI CTAG 3 cut(s) 344, 348, 1026
StyD4I CCNGG 2 cut(s) 118, 520
StyI CCWWGG 4 cut(s) 296, 653, 996, 1045
TaaI ACNGT 3 cut(s) 329, 408, 544
TaqI TCGA 5 cut(s) 10, 218, 628, 982, 1198
TasI AATT 8 cut(s) 281, 386, 491, 618, 631, 827, 876, 1021
TatI WGTACW 3 cut(s) 539, 1068, 1124
TauI GCSGC 1 cut(s) 1150
TfiI GAWTC 5 cut(s) 107, 683, 745, 1040, 1112
Tru1I TTAA 5 cut(s) 174, 354, 416, 668, 881
Tru9I TTAA 5 cut(s) 174, 354, 416, 668, 881
TscAI CASTG 4 cut(s) 55, 747, 811, 967
TseFI GTSAC 4 cut(s) 229, 802, 964, 1282
TseI GCWGC 4 cut(s) 689, 692, 957, 1118
Tsp45I GTSAC 4 cut(s) 229, 802, 964, 1282
TspDTI ATGAA 4 cut(s) 608, 737, 781, 1063
TspGWI ACGGA 1 cut(s) 797
TspRI CASTG 4 cut(s) 55, 747, 811, 967
VneI GTGCAC 1 cut(s) 902
VpaK11BI GGWCC 1 cut(s) 518
XapI RAATTY 5 cut(s) 386, 491, 631, 876, 1021
XceI RCATGY 1 cut(s) 825
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 3 cut(s) 344, 348, 1026
ZrmI AGTACT 3 cut(s) 541, 1070, 1126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.