Rw2G020350

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001114
Physical Location & Seq
Reverse (-)
27797438 .. 27798271
834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020350.1

Sequence Viewer

Length: 834 bp
ATGACAACAATATTTACAGCTGAGCAGCTAGAAATGGCTACCAACTGTTACCAAAGAATTTTACGCGATAAAGTAGTGGAGGGCCAAGGCGAGAGTTTTATAAATGAGATTATCGCTCTTGCCCAAATGAGTCATGAGAATTTGGTGAAGTTCATTGGCTGCTGTTTAGAGACTGATGCTCCTATTTTGGTTTATGAACTTGCCTCTAATGGAACCCTTTTCGACTGCATTAATCACACCAATGGGAAAGTTACATTGCCTTGGAATATCCTCCTAAAGATAGGGTCAGAATCTGCAGCTGCTCTTGCTTATATTCATTCTTCTGCAGGAATTCACTCCAAACGCATCATCCATGGCAATGTCAAGTCTTCCAACGTATTGTTAACTGATAATTTCACGGCCAAAGTCTCTGGTTTTTGGCCTTCGAGGTTAGTCCCTGCTAATGAATCTCAGATGGGCACATTGCTTCTAAGGACACTAGGTTATATGGACCCAGGGTATCTTTGCACAGGTCAACTGACAGATGAGAGTGATGTTTATAGCTTCGGAGTCGTTTTGGTAGAGATCTTAACCCGGGAAAAGCCTATTTCATTTGACAGACCAGAAAGTCAAAGAATCATTGCCTCTCATTTTGTTTCATCCATTGAATTAAAAGATGGTTTCTTTCATGTTGTTGATCCACTAGTTTTTAGCGAGGGAAATCGAGAACAAGTAAGAGCAGTTGCAGAGCTTGCAAAGAGATGCCTCCATATGAACAGTGAGGAAAGGCCTTCAATGAAAGAAGTCGCTGGAGAGTTGCAGAGGCTGTCTGGCATGTGTCCTACAGTAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

277

Amino Acids

30.67

Weight (kDa)

5.52

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 19 - 265 2.8e-31 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 21 - 262 3.9e-29 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 101
AasI GACNNNNNNGTC 1 cut(s) 606
AccII CGCG 1 cut(s) 66
AclWI GGATC 1 cut(s) 671
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 3 cut(s) 57, 139, 330
AgsI TTSAA 2 cut(s) 647, 774
AhlI ACTAGT 1 cut(s) 682
AjnI CCWGG 1 cut(s) 493
AluBI AGCT 5 cut(s) 20, 28, 299, 543, 730
AluI AGCT 5 cut(s) 20, 28, 299, 543, 730
Alw26I GTCTC 2 cut(s) 164, 412
AlwI GGATC 1 cut(s) 671
AlwNI CAGNNNCTG 2 cut(s) 293, 805
Ama87I CYCGRG 1 cut(s) 573
AoxI GGCC 4 cut(s) 82, 399, 419, 767
ApeKI GCWGC 4 cut(s) 25, 159, 296, 299
ApoI RAATTY 3 cut(s) 57, 139, 330
AseI ATTAAT 1 cut(s) 231
AspS9I GGNCC 2 cut(s) 82, 490
AsuC2I CCSGG 2 cut(s) 574, 575
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 573
AvaII GGWCC 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 360
BbvI GCAGC 4 cut(s) 37, 146, 286, 308
BccI CCATC 2 cut(s) 448, 650
BceAI ACGGC 1 cut(s) 414
BciT130I CCWGG 1 cut(s) 495
BcnI CCSGG 2 cut(s) 574, 575
BcoDI GTCTC 2 cut(s) 164, 412
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 3 cut(s) 29, 479, 683
BfmI CTRYAG 3 cut(s) 294, 324, 822
BglII AGATCT 1 cut(s) 564
BisI GCNGC 4 cut(s) 26, 160, 297, 300
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 4 cut(s) 27, 161, 298, 301
Bme1390I CCNGG 3 cut(s) 495, 574, 575
Bme18I GGWCC 1 cut(s) 490
BmeT110I CYCGRG 1 cut(s) 573
BmgT120I GGNCC 2 cut(s) 82, 490
BmiI GGNNCC 2 cut(s) 214, 492
BmrFI CCNGG 3 cut(s) 495, 574, 575
BmsI GCATC 3 cut(s) 166, 354, 731
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 1 cut(s) 810
Bpu1102I GCTNAGC 1 cut(s) 21
BpuMI CCSGG 2 cut(s) 574, 575
BsaJI CCNNGG 6 cut(s) 85, 260, 352, 493, 494, 573
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bse3DI GCAATG 4 cut(s) 254, 364, 461, 618
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 6 cut(s) 85, 260, 352, 493, 494, 573
BseGI GGATG 2 cut(s) 348, 638
BseMI GCAATG 4 cut(s) 254, 364, 461, 618
BseMII CTCAG 2 cut(s) 12, 464
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 4 cut(s) 37, 146, 286, 308
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 4 cut(s) 84, 401, 421, 769
BsiHKCI CYCGRG 1 cut(s) 573
BsiSI CCGG 1 cut(s) 574
BslFI GGGAC 1 cut(s) 419
BsmAI GTCTC 2 cut(s) 164, 412
BsmFI GGGAC 1 cut(s) 419
BsnI GGCC 4 cut(s) 84, 401, 421, 769
BsoBI CYCGRG 1 cut(s) 573
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 2 cut(s) 564, 676
Bsp1720I GCTNAGC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 352
BspANI GGCC 4 cut(s) 84, 401, 421, 769
BspCNI CTCAG 2 cut(s) 13, 463
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 133
BspLI GGNNCC 2 cut(s) 214, 492
BspMAI CTGCAG 2 cut(s) 298, 328
BspPI GGATC 1 cut(s) 671
BsrDI GCAATG 4 cut(s) 254, 364, 461, 618
BssECI CCNNGG 6 cut(s) 85, 260, 352, 493, 494, 573
BssMI GATC 2 cut(s) 564, 676
BssT1I CCWWGG 3 cut(s) 85, 260, 352
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 3 cut(s) 47, 758, 826
BstAPI GCANNNNNTGC 1 cut(s) 731
BstC8I GCNNGC 1 cut(s) 732
BstDEI CTNAG 3 cut(s) 21, 450, 470
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 2 cut(s) 348, 638
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 2 cut(s) 567, 679
BstMAI GTCTC 2 cut(s) 164, 412
BstMBI GATC 2 cut(s) 564, 676
BstMWI GCNNNNNNNGC 2 cut(s) 305, 731
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 1 cut(s) 817
BstSCI CCNGG 3 cut(s) 493, 572, 573
BstSFI CTRYAG 3 cut(s) 294, 324, 822
BstSLI GKGCMC 1 cut(s) 461
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 4 cut(s) 37, 146, 286, 308
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BsuRI GGCC 4 cut(s) 84, 401, 421, 769
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 2 cut(s) 348, 638
BtsIMutI CAGTG 1 cut(s) 763
Cac8I GCNNGC 1 cut(s) 732
CaiI CAGNNNCTG 2 cut(s) 293, 805
CciI TCATGA 1 cut(s) 133
Cfr13I GGNCC 2 cut(s) 82, 490
Cfr9I CCCGGG 1 cut(s) 573
CviAII CATG 4 cut(s) 134, 353, 668, 814
DdeI CTNAG 3 cut(s) 21, 450, 470
DpnI GATC 2 cut(s) 566, 678
DpnII GATC 2 cut(s) 564, 676
DrdI GACNNNNNNGTC 1 cut(s) 606
DseDI GACNNNNNNGTC 1 cut(s) 606
EaeI YGGCCR 1 cut(s) 399
Eco130I CCWWGG 3 cut(s) 85, 260, 352
Eco147I AGGCCT 1 cut(s) 769
Eco47I GGWCC 1 cut(s) 490
Eco88I CYCGRG 1 cut(s) 573
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 493
EcoT14I CCWWGG 3 cut(s) 85, 260, 352
ErhI CCWWGG 3 cut(s) 85, 260, 352
FaeI CATG 4 cut(s) 137, 356, 671, 817
FaqI GGGAC 1 cut(s) 419
FatI CATG 4 cut(s) 133, 352, 667, 813
FauNDI CATATG 1 cut(s) 750
Fnu4HI GCNGC 4 cut(s) 26, 160, 297, 300
FokI GGATG 2 cut(s) 335, 625
Fsp4HI GCNGC 4 cut(s) 26, 160, 297, 300
FspBI CTAG 3 cut(s) 29, 479, 683
GluI GCNGC 4 cut(s) 26, 160, 297, 300
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 4 cut(s) 84, 401, 421, 769
HapII CCGG 1 cut(s) 574
Hin1II CATG 4 cut(s) 137, 356, 671, 817
HincII GTYRAC 2 cut(s) 384, 515
HindII GTYRAC 2 cut(s) 384, 515
HinfI GANTC 5 cut(s) 130, 290, 446, 549, 615
HpaI GTTAAC 1 cut(s) 384
HpaII CCGG 1 cut(s) 574
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 384, 515
Hpy188I TCNGA 4 cut(s) 289, 453, 548, 833
Hpy188III TCNNGA 2 cut(s) 134, 704
Hpy8I GTNNAC 2 cut(s) 384, 515
HpyAV CCTTC 2 cut(s) 432, 780
HpyCH4III ACNGT 3 cut(s) 47, 758, 826
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 7 cut(s) 228, 296, 326, 507, 725, 734, 799
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 731
HpyF3I CTNAG 3 cut(s) 21, 450, 470
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 4 cut(s) 137, 356, 671, 817
KspAI GTTAAC 1 cut(s) 384
Kzo9I GATC 2 cut(s) 564, 676
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 4 cut(s) 37, 146, 286, 308
LweI GCATC 3 cut(s) 166, 354, 731
MaeI CTAG 3 cut(s) 29, 479, 683
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 2 cut(s) 47, 250
MalI GATC 2 cut(s) 566, 678
MboI GATC 2 cut(s) 564, 676
MboII GAAGA 2 cut(s) 312, 360
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 5 cut(s) 57, 139, 330, 391, 647
MlyI GAGTC 2 cut(s) 139, 558
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 9 cut(s) 73, 214, 281, 420, 634, 688, 754, 755, 795
MseI TTAA 4 cut(s) 231, 383, 569, 650
MslI CAYNNNNRTG 2 cut(s) 240, 357
MspA1I CMGCKG 2 cut(s) 20, 299
MspI CCGG 1 cut(s) 574
MspR9I CCNGG 3 cut(s) 495, 574, 575
MvaI CCWGG 1 cut(s) 495
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 2 cut(s) 305, 731
NciI CCSGG 2 cut(s) 574, 575
NcoI CCATGG 1 cut(s) 352
NdeI CATATG 1 cut(s) 750
NdeII GATC 2 cut(s) 564, 676
NlaIII CATG 4 cut(s) 137, 356, 671, 817
NlaIV GGNNCC 2 cut(s) 214, 492
NspI RCATGY 1 cut(s) 817
PagI TCATGA 1 cut(s) 133
PasI CCCWGGG 1 cut(s) 494
PceI AGGCCT 1 cut(s) 769
PfeI GAWTC 3 cut(s) 290, 446, 615
PkrI GCNGC 4 cut(s) 27, 161, 298, 301
PleI GAGTC 2 cut(s) 138, 557
PpsI GAGTC 2 cut(s) 138, 557
PshBI ATTAAT 1 cut(s) 231
PsiI TTATAA 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 2 cut(s) 214, 492
PspPI GGNCC 2 cut(s) 82, 490
PstI CTGCAG 2 cut(s) 298, 328
PstNI CAGNNNCTG 2 cut(s) 293, 805
PsuI RGATCY 1 cut(s) 564
PvuII CAGCTG 2 cut(s) 20, 299
RseI CAYNNNNRTG 2 cut(s) 240, 357
SaqAI TTAA 4 cut(s) 231, 383, 569, 650
SatI GCNGC 4 cut(s) 26, 160, 297, 300
Sau3AI GATC 2 cut(s) 564, 676
Sau96I GGNCC 2 cut(s) 82, 490
SchI GAGTC 2 cut(s) 139, 558
ScrFI CCNGG 3 cut(s) 495, 574, 575
SduI GDGCHC 1 cut(s) 461
SetI ASST 9 cut(s) 22, 30, 301, 378, 431, 484, 514, 545, 732
SfaNI GCATC 3 cut(s) 166, 354, 731
SfcI CTRYAG 3 cut(s) 294, 324, 822
SinI GGWCC 1 cut(s) 490
SmaI CCCGGG 1 cut(s) 575
SmiMI CAYNNNNRTG 2 cut(s) 240, 357
SpeI ACTAGT 1 cut(s) 682
Sse9I AATT 5 cut(s) 57, 139, 330, 391, 647
SseBI AGGCCT 1 cut(s) 769
SspI AATATT 1 cut(s) 12
SspMI CTAG 3 cut(s) 29, 479, 683
StuI AGGCCT 1 cut(s) 769
StyD4I CCNGG 3 cut(s) 493, 572, 573
StyI CCWWGG 3 cut(s) 85, 260, 352
TaaI ACNGT 3 cut(s) 47, 758, 826
TaiI ACGT 1 cut(s) 378
TaqI TCGA 3 cut(s) 222, 425, 703
TasI AATT 5 cut(s) 57, 139, 330, 391, 647
TfiI GAWTC 3 cut(s) 290, 446, 615
Tru1I TTAA 4 cut(s) 231, 383, 569, 650
Tru9I TTAA 4 cut(s) 231, 383, 569, 650
TscAI CASTG 1 cut(s) 763
TseI GCWGC 4 cut(s) 25, 159, 296, 299
TspDTI ATGAA 9 cut(s) 142, 210, 305, 459, 579, 627, 656, 767, 791
TspMI CCCGGG 1 cut(s) 573
TspRI CASTG 1 cut(s) 763
VpaK11BI GGWCC 1 cut(s) 490
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 3 cut(s) 57, 139, 330
XceI RCATGY 1 cut(s) 817
XmaI CCCGGG 1 cut(s) 573
XspI CTAG 3 cut(s) 29, 479, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.