RchiOBHm_Chr5g0047041

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001114
Physical Location & Seq
Forward (+)
43291697 .. 43292494
798 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32498

Sequence Viewer

Length: 798 bp
ATGGCTGTTGACTATCATACGAAGAGTCACATTTTTGGTCCAGGAGAGGGTGGAGTCGATCGTTTCATAGATGAGATTATCACTCTTACCGGAATCAACCATCAAAATATGGTCAAGTTCTTGGGCTGCTGTCTAGAGACTGAAGCTCCCATACTGGTCTATGAACTTGCCTCCAATGGGACCCTTTTGGACTACATTCATCACACCAATGGGAAAAGATCATTGTCTTGGGAAACGCTTCTAAAGATAGCCTCAGAATCTGCAGCAGGTCTTAATTATCTGCATTCTGCGTCAATCATTCATGGAAATGTAAAGTCTTCCAACGTTTTACTAACTGATCGTTTCGTTGCCAAAGTTGCCGGATTTGGACCTCCGAGGTTATTTCCTACTAATGAATCCCAGATAAGCACACTGCTGCAGAGGACACTTGGCTATCTAGACCCGGAGTATCTTCATAAAGGTCAACTGACAGATAAGAGTGATGTCTACAGCTTTGGAATGGTTTTGTTAGAGATTGTAACAGGAGAGAAGCCAATATCATTTGGTAGACCAGAAAGTCAGAGAATCATATCTTCCCATTTTATTTCGTCCATGGAATTACAAAGTGGTCTCTTTCATGAGATTGTTTGGCCACAAATTGTAAGAGAAGGAGACAGAGAACAAGTCAGAGCAGTAGCAGAGCTTGCAAAAAGGTGCCTCAAGCTGAGTAGTGCAGAAAGACCAACAATGGAAGAAGTGGCCCGAGAGCTAAAGAGGCTGTCTGACAGCCACCAAGTACAAGTTGGCCGGCCGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

265

Amino Acids

29.53

Weight (kDa)

6.26

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 20 - 250 8.3e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 20 - 247 7.5e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 555
Acc36I ACCTGC 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 693
AccI GTMKAC 2 cut(s) 486, 547
AciI CCGC 1 cut(s) 791
AclI AACGTT 1 cut(s) 324
AcoI YGGCCR 3 cut(s) 629, 784, 788
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 2 cut(s) 47, 177
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 5 cut(s) 146, 492, 682, 703, 748
AluI AGCT 5 cut(s) 146, 492, 682, 703, 748
Alw26I GTCTC 3 cut(s) 131, 614, 645
AlwNI CAGNNNCTG 1 cut(s) 260
Ama87I CYCGRG 1 cut(s) 741
AoxI GGCC 4 cut(s) 629, 738, 784, 788
ApeKI GCWGC 3 cut(s) 126, 263, 415
Asp700I GAANNNNTTC 1 cut(s) 237
AspS9I GGNCC 4 cut(s) 38, 180, 368, 739
AsuC2I CCSGG 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 3 cut(s) 38, 180, 368
BalI TGGCCA 1 cut(s) 631
BanI GGYRCC 1 cut(s) 693
BarI GAAGNNNNNNTAC 2 cut(s) 135, 167
BbsI GAAGAC 1 cut(s) 309
BbvI GCAGC 3 cut(s) 113, 275, 402
BccI CCATC 1 cut(s) 108
BciT130I CCWGG 1 cut(s) 42
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 3 cut(s) 131, 614, 645
BfaI CTAG 2 cut(s) 134, 437
BfmI CTRYAG 3 cut(s) 261, 416, 487
BfuAI ACCTGC 1 cut(s) 257
BisI GCNGC 4 cut(s) 127, 264, 416, 791
BlsI GCNGC 4 cut(s) 128, 265, 417, 792
Bme1390I CCNGG 2 cut(s) 42, 443
Bme18I GGWCC 3 cut(s) 38, 180, 368
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 4 cut(s) 38, 180, 368, 739
BmiI GGNNCC 3 cut(s) 181, 182, 695
BmrFI CCNGG 2 cut(s) 42, 443
BpiI GAAGAC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 2 cut(s) 374, 591
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 177
Bse118I RCCGGY 1 cut(s) 786
Bse1I ACTGG 1 cut(s) 159
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 2 cut(s) 374, 591
BseLI CCNNNNNNNGG 2 cut(s) 47, 177
BseMII CTCAG 2 cut(s) 267, 695
BseNI ACTGG 1 cut(s) 159
BseX3I CGGCCG 1 cut(s) 788
BseXI GCAGC 3 cut(s) 113, 275, 402
BsgI GTGCAG 1 cut(s) 732
Bsh1285I CGRYCG 2 cut(s) 61, 791
BshFI GGCC 4 cut(s) 631, 740, 786, 790
BshNI GGYRCC 1 cut(s) 693
BsiEI CGRYCG 2 cut(s) 61, 791
BsiHKCI CYCGRG 1 cut(s) 741
BsiSI CCGG 4 cut(s) 90, 360, 443, 787
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 47, 177
BsmAI GTCTC 3 cut(s) 131, 614, 645
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 1 cut(s) 283
BsnI GGCC 4 cut(s) 631, 740, 786, 790
Bso31I GGTCTC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 741
Bsp143I GATC 3 cut(s) 58, 218, 337
Bsp19I CCATGG 1 cut(s) 591
BspACI CCGC 1 cut(s) 791
BspANI GGCC 4 cut(s) 631, 740, 786, 790
BspCNI CTCAG 2 cut(s) 266, 696
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 3 cut(s) 181, 182, 695
BspMAI CTGCAG 2 cut(s) 265, 420
BspMI ACCTGC 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 693
BspTNI GGTCTC 1 cut(s) 614
BsrFI RCCGGY 1 cut(s) 786
BsrI ACTGG 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 786
BssECI CCNNGG 2 cut(s) 374, 591
BssMI GATC 3 cut(s) 58, 218, 337
BssT1I CCWWGG 1 cut(s) 591
Bst2UI CCWGG 1 cut(s) 42
Bst6I CTCTTC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 683
BstC8I GCNNGC 2 cut(s) 684, 788
BstDEI CTNAG 2 cut(s) 253, 704
BstDSI CCRYGG 1 cut(s) 591
BstKTI GATC 3 cut(s) 61, 221, 340
BstMAI GTCTC 3 cut(s) 131, 614, 645
BstMBI GATC 3 cut(s) 58, 218, 337
BstMCI CGRYCG 2 cut(s) 61, 791
BstMWI GCNNNNNNNGC 3 cut(s) 356, 683, 754
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 2 cut(s) 40, 441
BstSFI CTRYAG 3 cut(s) 261, 416, 487
BstV1I GCAGC 3 cut(s) 113, 275, 402
BstV2I GAAGAC 1 cut(s) 309
BstZI CGGCCG 1 cut(s) 788
BsuRI GGCC 4 cut(s) 631, 740, 786, 790
BtgI CCRYGG 1 cut(s) 591
BtsI GCAGTG 1 cut(s) 410
BtsIMutI CAGTG 1 cut(s) 410
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 2 cut(s) 684, 788
CaiI CAGNNNCTG 1 cut(s) 260
CciI TCATGA 1 cut(s) 616
Cfr10I RCCGGY 1 cut(s) 786
Cfr13I GGNCC 4 cut(s) 38, 180, 368, 739
CseI GACGC 1 cut(s) 279
Csp6I GTAC 1 cut(s) 776
CviAII CATG 4 cut(s) 302, 592, 617, 795
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 2 cut(s) 253, 704
DpnI GATC 3 cut(s) 60, 220, 339
DpnII GATC 3 cut(s) 58, 218, 337
DrdI GACNNNNNNGTC 1 cut(s) 555
DseDI GACNNNNNNGTC 1 cut(s) 555
EaeI YGGCCR 3 cut(s) 629, 784, 788
EagI CGGCCG 1 cut(s) 788
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
EclXI CGGCCG 1 cut(s) 788
Eco130I CCWWGG 1 cut(s) 591
Eco31I GGTCTC 1 cut(s) 614
Eco47I GGWCC 3 cut(s) 38, 180, 368
Eco52I CGGCCG 1 cut(s) 788
Eco57I CTGAAG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 741
EcoO109I RGGNCCY 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 591
ErhI CCWWGG 1 cut(s) 591
FaeI CATG 4 cut(s) 305, 595, 620, 798
FaqI GGGAC 1 cut(s) 193
FatI CATG 4 cut(s) 301, 591, 616, 794
FblI GTMKAC 2 cut(s) 486, 547
Fnu4HI GCNGC 4 cut(s) 127, 264, 416, 791
FseI GGCCGGCC 1 cut(s) 790
Fsp4HI GCNGC 4 cut(s) 127, 264, 416, 791
FspBI CTAG 2 cut(s) 134, 437
GluI GCNGC 4 cut(s) 127, 264, 416, 791
HaeIII GGCC 4 cut(s) 631, 740, 786, 790
HapII CCGG 4 cut(s) 90, 360, 443, 787
HgaI GACGC 1 cut(s) 279
Hin1II CATG 4 cut(s) 305, 595, 620, 798
HincII GTYRAC 2 cut(s) 10, 464
HindII GTYRAC 2 cut(s) 10, 464
HinfI GANTC 6 cut(s) 25, 54, 93, 257, 395, 564
HpaII CCGG 4 cut(s) 90, 360, 443, 787
Hpy166II GTNNAC 4 cut(s) 10, 464, 487, 548
Hpy188I TCNGA 5 cut(s) 256, 375, 561, 668, 763
Hpy188III TCNNGA 3 cut(s) 134, 437, 617
Hpy8I GTNNAC 4 cut(s) 10, 464, 487, 548
HpyAV CCTTC 1 cut(s) 641
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 5 cut(s) 263, 283, 418, 686, 713
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 683, 754
HpyF3I CTNAG 2 cut(s) 253, 704
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 4 cut(s) 305, 595, 620, 798
KflI GGGWCCC 1 cut(s) 180
KroI GCCGGC 1 cut(s) 786
KroNI GCCGGC 1 cut(s) 788
Kzo9I GATC 3 cut(s) 58, 218, 337
LmnI GCTCC 1 cut(s) 151
Lsp1109I GCAGC 3 cut(s) 113, 275, 402
MaeI CTAG 2 cut(s) 134, 437
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 26, 517
MalI GATC 3 cut(s) 60, 220, 339
MboI GATC 3 cut(s) 58, 218, 337
MboII GAAGA 5 cut(s) 34, 309, 443, 564, 743
MlsI TGGCCA 1 cut(s) 631
MluCI AATT 3 cut(s) 274, 596, 636
MluNI TGGCCA 1 cut(s) 631
MlyI GAGTC 2 cut(s) 34, 63
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 8 cut(s) 40, 181, 262, 369, 381, 414, 707, 747
Mox20I TGGCCA 1 cut(s) 631
MroNI GCCGGC 1 cut(s) 786
MroXI GAANNNNTTC 1 cut(s) 237
MscI TGGCCA 1 cut(s) 631
MseI TTAA 1 cut(s) 273
MslI CAYNNNNRTG 2 cut(s) 207, 306
Msp20I TGGCCA 1 cut(s) 631
MspI CCGG 4 cut(s) 90, 360, 443, 787
MspR9I CCNGG 2 cut(s) 42, 443
Mva1269I GAATGC 1 cut(s) 283
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 356, 683, 754
NaeI GCCGGC 1 cut(s) 788
NciI CCSGG 1 cut(s) 443
NcoI CCATGG 1 cut(s) 591
NdeII GATC 3 cut(s) 58, 218, 337
NgoMIV GCCGGC 1 cut(s) 786
NlaIII CATG 4 cut(s) 305, 595, 620, 798
NlaIV GGNNCC 3 cut(s) 181, 182, 695
NmuCI GTSAC 1 cut(s) 26
PagI TCATGA 1 cut(s) 616
PctI GAATGC 1 cut(s) 283
PdiI GCCGGC 1 cut(s) 788
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 4 cut(s) 93, 257, 395, 564
PfoI TCCNGGA 1 cut(s) 40
PkrI GCNGC 4 cut(s) 128, 265, 417, 792
Ple19I CGATCG 1 cut(s) 61
PleI GAGTC 2 cut(s) 33, 62
PpsI GAGTC 2 cut(s) 33, 62
PpuMI RGGWCCY 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 3 cut(s) 181, 182, 695
PspPI GGNCC 4 cut(s) 38, 180, 368, 739
PspPPI RGGWCCY 1 cut(s) 180
PstI CTGCAG 2 cut(s) 265, 420
PstNI CAGNNNCTG 1 cut(s) 260
PvuI CGATCG 1 cut(s) 61
RigI GGCCGGCC 1 cut(s) 790
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
RseI CAYNNNNRTG 2 cut(s) 207, 306
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 4 cut(s) 127, 264, 416, 791
Sau3AI GATC 3 cut(s) 58, 218, 337
Sau96I GGNCC 4 cut(s) 38, 180, 368, 739
SchI GAGTC 2 cut(s) 34, 63
ScrFI CCNGG 2 cut(s) 42, 443
SfcI CTRYAG 3 cut(s) 261, 416, 487
SinI GGWCC 3 cut(s) 38, 180, 368
SmiMI CAYNNNNRTG 2 cut(s) 207, 306
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
Sse9I AATT 3 cut(s) 274, 596, 636
SsiI CCGC 1 cut(s) 791
SspMI CTAG 2 cut(s) 134, 437
StyD4I CCNGG 2 cut(s) 40, 441
StyI CCWWGG 1 cut(s) 591
TaiI ACGT 1 cut(s) 327
TaqI TCGA 1 cut(s) 57
TasI AATT 3 cut(s) 274, 596, 636
TatI WGTACW 1 cut(s) 775
TauI GCSGC 1 cut(s) 793
TfiI GAWTC 4 cut(s) 93, 257, 395, 564
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 417
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 3 cut(s) 126, 263, 415
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 7 cut(s) 55, 177, 188, 290, 408, 443, 605
TspRI CASTG 1 cut(s) 417
VpaK11BI GGWCC 3 cut(s) 38, 180, 368
XbaI TCTAGA 2 cut(s) 133, 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 779
XmiI GTMKAC 2 cut(s) 486, 547
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 2 cut(s) 134, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.