Rorug01G0444000

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001114
Physical Location & Seq
Reverse (-)
54055511 .. 54057637
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0444000.1

Sequence Viewer

Length: 711 bp
ATGGAAGACCCAAAATCAAAACGTGTTGAGAGCAGAGAACCACACATGACAACAACAACAACGCCACCTCCACATGGAGCAAGCCCACCCCAAAAACCCACTTGTCTTCCTCGCCAAAACCAGACCCAGAAGCAGACCGACGTCAAGAAGCGAAAGCTTTCCAACACCAACATGGAGAAAGACTCCCCCTACTTCAAAATCCGCGCTCTCCTTCAACAACTCCGTCCTCATTTTATTGAGGTTCTTCAAACACCTGATTTTCGGAACTGTAAAGCAGCTGATGAGATTCAAAAACAGGTGAAGCTTCTCATAGCTCTAAACAAACAAATGACCATGGATACTGGAACACAGAAGAAGTTTGCATCAGAACATCAACAGCATTCGTCCCATACCAGGCTGCCTGCAAAAGAAAACAAAGATGGATTAGCAACACAGCAGGTTCAACCTAGATGCTCTGTTGAAAAACAACTGCCTAGTGCAAAATGGACTCATTACATCCCAGATAAGAAATCATTTGTCAGCAGCCAGCTACCGGGAAGCTACATTGTGGGTGGTTCGGTTTTTGGTTGGAACTTCATCACATTTTCCGGCAGTAAACCAGTCTATTATGGTGTAACAAAGGAGGCATATCGAAGCAGCCACATTAGATCAGGAGATGCTTCTATGCTTTCAACAGATGGTTCTTCTGGACAAGGGAGACCATCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

236

Amino Acids

26.54

Weight (kDa)

9.81

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc36I ACCTGC 1 cut(s) 427
AccII CGCG 1 cut(s) 204
AciI CCGC 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 141
AfiI CCNNNNNNNGG 3 cut(s) 74, 393, 532
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 7 cut(s) 196, 215, 248, 290, 443, 461, 672
AjnI CCWGG 1 cut(s) 392
AluBI AGCT 6 cut(s) 157, 278, 304, 314, 529, 540
AluI AGCT 6 cut(s) 157, 278, 304, 314, 529, 540
Alw26I GTCTC 1 cut(s) 691
ApeKI GCWGC 4 cut(s) 275, 397, 522, 636
Asp700I GAANNNNTTC 1 cut(s) 157
AspLEI GCGC 1 cut(s) 206
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 1 cut(s) 310
BbsI GAAGAC 2 cut(s) 12, 98
BbvI GCAGC 4 cut(s) 287, 384, 534, 648
BccI CCATC 3 cut(s) 413, 671, 709
BciT130I CCWGG 1 cut(s) 394
BciVI GTATCC 1 cut(s) 331
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 2 cut(s) 447, 474
BfuAI ACCTGC 1 cut(s) 427
BfuI GTATCC 1 cut(s) 331
BisI GCNGC 4 cut(s) 276, 398, 523, 637
BlsI GCNGC 4 cut(s) 277, 399, 524, 638
Bme1390I CCNGG 2 cut(s) 394, 534
BmrFI CCNGG 2 cut(s) 394, 534
BmsI GCATC 3 cut(s) 371, 440, 646
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 12, 98
BplI GAGNNNNNCTC 2 cut(s) 167, 199
BpuMI CCSGG 1 cut(s) 534
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 691
BsaJI CCNNGG 1 cut(s) 333
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 393, 532
Bse1I ACTGG 2 cut(s) 346, 599
BseBI CCWGG 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 333
BseGI GGATG 1 cut(s) 495
BseLI CCNNNNNNNGG 3 cut(s) 74, 393, 532
BseNI ACTGG 2 cut(s) 346, 599
BseXI GCAGC 4 cut(s) 287, 384, 534, 648
Bsh1236I CGCG 1 cut(s) 204
BsiSI CCGG 2 cut(s) 533, 588
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 3 cut(s) 74, 393, 532
BsmAI GTCTC 1 cut(s) 691
BsmFI GGGAC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 379
Bso31I GGTCTC 1 cut(s) 691
Bsp143I GATC 1 cut(s) 647
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 1 cut(s) 202
BspFNI CGCG 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 427
BspTNI GGTCTC 1 cut(s) 691
BsrI ACTGG 2 cut(s) 346, 599
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 1 cut(s) 647
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 394
Bst4CI ACNGT 1 cut(s) 269
BstACI GRCGYC 1 cut(s) 141
BstC8I GCNNGC 3 cut(s) 82, 402, 527
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 495
BstFNI CGCG 1 cut(s) 204
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 1 cut(s) 650
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 1 cut(s) 647
BstNI CCWGG 1 cut(s) 394
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 392, 532
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 4 cut(s) 287, 384, 534, 648
BstV2I GAAGAC 2 cut(s) 12, 98
BsuI GTATCC 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 495
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 3 cut(s) 82, 402, 527
CfoI GCGC 1 cut(s) 206
CviAII CATG 4 cut(s) 46, 74, 172, 334
DpnI GATC 1 cut(s) 649
DpnII GATC 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 691
EcoRII CCWGG 1 cut(s) 392
EcoT14I CCWWGG 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 4 cut(s) 49, 77, 175, 337
FaqI GGGAC 1 cut(s) 370
FatI CATG 4 cut(s) 45, 73, 171, 333
Fnu4HI GCNGC 4 cut(s) 276, 398, 523, 637
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 4 cut(s) 276, 398, 523, 637
FspBI CTAG 2 cut(s) 447, 474
GlaI GCGC 1 cut(s) 205
GluI GCNGC 4 cut(s) 276, 398, 523, 637
HapII CCGG 2 cut(s) 533, 588
HhaI GCGC 1 cut(s) 206
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 4 cut(s) 49, 77, 175, 337
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HindIII AAGCTT 2 cut(s) 155, 302
HinfI GANTC 3 cut(s) 182, 286, 487
HpaII CCGG 2 cut(s) 533, 588
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 1 cut(s) 596
Hpy188I TCNGA 2 cut(s) 264, 367
Hpy188III TCNNGA 3 cut(s) 145, 651, 687
Hpy8I GTNNAC 1 cut(s) 596
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 2 cut(s) 22, 141
HpyCH4V TGCA 3 cut(s) 362, 404, 479
HpySE526I ACGT 2 cut(s) 22, 141
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 4 cut(s) 49, 77, 175, 337
HspAI GCGC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 647
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 4 cut(s) 287, 384, 534, 648
LweI GCATC 3 cut(s) 371, 440, 646
MaeI CTAG 2 cut(s) 447, 474
MaeII ACGT 2 cut(s) 22, 141
MaeIII GTNAC 1 cut(s) 613
MalI GATC 1 cut(s) 649
MboI GATC 1 cut(s) 647
MboII GAAGA 5 cut(s) 17, 98, 236, 364, 675
MlyI GAGTC 2 cut(s) 176, 481
MmeI TCCRAC 2 cut(s) 186, 548
MnlI CCTC 5 cut(s) 78, 120, 232, 237, 616
MroXI GAANNNNTTC 1 cut(s) 157
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 2 cut(s) 533, 588
MspR9I CCNGG 2 cut(s) 394, 534
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 394
MvnI CGCG 1 cut(s) 204
NciI CCSGG 1 cut(s) 534
NcoI CCATGG 1 cut(s) 333
NdeII GATC 1 cut(s) 647
NlaIII CATG 4 cut(s) 49, 77, 175, 337
PctI GAATGC 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 286
PkrI GCNGC 4 cut(s) 277, 399, 524, 638
PleI GAGTC 2 cut(s) 176, 481
PpsI GAGTC 2 cut(s) 176, 481
PshAI GACNNNNGTC 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 392
PspGI CCWGG 1 cut(s) 392
PvuII CAGCTG 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 170
SatI GCNGC 4 cut(s) 276, 398, 523, 637
Sau3AI GATC 1 cut(s) 647
SchI GAGTC 2 cut(s) 176, 481
ScrFI CCNGG 2 cut(s) 394, 534
SfaNI GCATC 3 cut(s) 371, 440, 646
SmiMI CAYNNNNRTG 1 cut(s) 170
SsiI CCGC 1 cut(s) 202
SspMI CTAG 2 cut(s) 447, 474
StyD4I CCNGG 2 cut(s) 392, 532
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 2 cut(s) 25, 144
TaqI TCGA 1 cut(s) 631
TaqII GACCGA 1 cut(s) 152
TfiI GAWTC 1 cut(s) 286
TseI GCWGC 4 cut(s) 275, 397, 522, 636
TspDTI ATGAA 1 cut(s) 565
TspGWI ACGGA 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 157
XspI CTAG 2 cut(s) 447, 474
ZraI GACGTC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.