RLG00000019139

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001114
Physical Location & Seq
Reverse (-)
47191938 .. 47193578
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019139

Sequence Viewer

Length: 1494 bp
ATGTTGCACTCTAACAAGGTCCAGGCTGCAATGACTTTGACAGATGGCAGCCAGATGGTTACAACCTCCAACACTAATGCAAATAACTTTATAGTCATAGGTTATGAACCATTTGGTTTTATAGGTTATGAACCATTAACTTTGAACATCAATAGCTCTCTGGCTGATCAGGAGGATGGGGCTTGTTCTGGTATTGGCTGCGGCCATTCCTCCATTCCAAGAGGCATCGAGTACTTTTTTCCAAAGGAGGCAGAGAGTATAGTAAAGATAACTTTATTATCCATGTATTTTTGGTTTACCATGACACTTTGTGTTACTGGTACACCATCCAAGTCCAGCCTCATCTTTCCTTTGCCCATGGTAGCAAACATCAGTAGCGTGCGACAGCCATACTATGAAAAACTAGCTGTCTTTCACAACTGTGATAAACGATCTGTACGTTCACTCATCGAAGTTGGCACACATGATAAATTGGTATACCACATGAAAAGTGGTAGAGGCTACCTTCCAAACAGCGAAGTCTTTCGGGCAAATTTTACTCTAGGTCTTGGATTTTGTTGCTTATTTCTTTTGTTTGGGATCACTGGGATTTATTCCAGTGTGAAGGCACAGAAGTTGATTAAGCACAAATCCAAGTTCTTCCAGAAAAATAGAGGGTTATTATTAGAGCAGCATATTAATGCTTCAACCAGAGGATGTACAACAATTTTTACATCTAAGGAGCTAGAAATGACCATCAACTGTCATAAGAAGTGTCACATATTTGGTCCAGAAGATTATGGAGTCGAGCATTTCATAAATGAGATTATCAGTCTTACCCAAATGAACCATCAAAATGTGATCAAGTTGTTGGGCTGCTGTCTGGAGACTGAAGCTCCTATACTGGTCTATGAACTGGCCTCTGATGGGACCCTTTTTGACTACATTTATCATAACAGTGGGAAGAGTTCATTGCCTTGGGATACCCTTCTAAATATAGCCTCAGAATCAGCAGCTGCCCTTGCTTATCTGCATTCTGCATCAATCATCTATGGAAATGTCAAGTCTTCCAACATATTGTTAACTCATTGTTTTGTGGCCAAAGTCATCGGTTTTGGACCTTCGAGGTTAGTCCCTAGTAATGAATCACAGATAACCACATGGGTTCGGAGGACACTAGGCTATCTAGACCCGAAGTATTTTCATAAAGGCCAATTGACAGATAAGAGCGATGTTTACAGCTTTGGAATGGTTTTGTTAGAGATTGTAACAGGAGAGAAGCCAATATCATTTGGTAGACCAGAAAGTCAAAGAATCATGTCTTCCCATGTTGTATCGTCCATGGAGTTACAAAGTGGTCTGTTTGATCAGATTGTTGGGTCACAAGTCATAAAACAGGGACACAGAGAACAAGTCAGAGCAGTAGCTGAGCTTGCAAAGAGATGCCTCAGTTTGAGTAGTGCAGAAAGACCTGCTATGGAAGAAGTGGCCAGGAAGTTGAAAATGTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

498

Amino Acids

55.28

Weight (kDa)

8.44

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 259 - 490 9.6e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 262 - 493 7.2e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1284
Acc36I ACCTGC 1 cut(s) 1459
AccI GTMKAC 2 cut(s) 477, 1276
AciI CCGC 1 cut(s) 201
AclWI GGATC 1 cut(s) 587
AcoI YGGCCR 3 cut(s) 202, 1077, 1467
AcsI RAATTY 1 cut(s) 532
AcuI CTGAAG 1 cut(s) 891
AdeI CACNNNGTG 1 cut(s) 311
AfaI GTAC 4 cut(s) 233, 322, 438, 700
AfiI CCNNNNNNNGG 1 cut(s) 906
AgsI TTSAA 3 cut(s) 145, 687, 1480
AjnI CCWGG 2 cut(s) 21, 1469
AleI CACNNNNGTG 1 cut(s) 420
AluBI AGCT 8 cut(s) 156, 407, 724, 875, 995, 1221, 1406, 1411
AluI AGCT 8 cut(s) 156, 407, 724, 875, 995, 1221, 1406, 1411
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 1 cut(s) 587
AlwNI CAGNNNCTG 2 cut(s) 995, 1406
AoxI GGCC 5 cut(s) 202, 897, 1077, 1189, 1467
ApeKI GCWGC 7 cut(s) 26, 48, 198, 670, 855, 992, 995
ApoI RAATTY 1 cut(s) 532
AseI ATTAAT 1 cut(s) 678
Asp700I GAANNNNTTC 1 cut(s) 522
AspS9I GGNCC 4 cut(s) 19, 767, 909, 1097
AvaII GGWCC 4 cut(s) 19, 767, 909, 1097
BalI TGGCCA 2 cut(s) 1079, 1469
BarI GAAGNNNNNNTAC 2 cut(s) 864, 896
BbsI GAAGAC 2 cut(s) 1038, 1293
BbvI GCAGC 7 cut(s) 13, 60, 185, 682, 842, 982, 1004
BccI CCATC 7 cut(s) 38, 49, 170, 334, 743, 837, 899
BciT130I CCWGG 2 cut(s) 23, 1471
BciVI GTATCC 1 cut(s) 955
BclI TGATCA 3 cut(s) 166, 840, 1345
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 6 cut(s) 404, 542, 725, 1116, 1157, 1166
BfuAI ACCTGC 1 cut(s) 1459
BfuI GTATCC 1 cut(s) 955
BisI GCNGC 8 cut(s) 27, 49, 199, 202, 671, 856, 993, 996
BlpI GCTNAGC 1 cut(s) 1407
BlsI GCNGC 8 cut(s) 28, 50, 200, 203, 672, 857, 994, 997
BmcAI AGTACT 1 cut(s) 233
Bme1390I CCNGG 2 cut(s) 23, 1471
Bme18I GGWCC 4 cut(s) 19, 767, 909, 1097
BmgT120I GGNCC 4 cut(s) 19, 767, 909, 1097
BmiI GGNNCC 2 cut(s) 910, 911
BmrFI CCNGG 2 cut(s) 23, 1471
BmrI ACTGGG 1 cut(s) 594
BmsI GCATC 3 cut(s) 234, 1028, 1412
BmuI ACTGGG 1 cut(s) 594
BpiI GAAGAC 2 cut(s) 1038, 1293
BpmI CTGGAG 1 cut(s) 884
Bpu1102I GCTNAGC 1 cut(s) 1407
BsaJI CCNNGG 3 cut(s) 357, 956, 1320
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bsc4I CCNNNNNNNGG 1 cut(s) 906
Bse1I ACTGG 5 cut(s) 322, 589, 597, 888, 900
Bse3DI GCAATG 2 cut(s) 36, 950
BseBI CCWGG 2 cut(s) 23, 1471
BseDI CCNNGG 3 cut(s) 357, 956, 1320
BseGI GGATG 3 cut(s) 181, 326, 701
BseLI CCNNNNNNNGG 1 cut(s) 906
BseMI GCAATG 2 cut(s) 36, 950
BseMII CTCAG 3 cut(s) 996, 1398, 1441
BseNI ACTGG 5 cut(s) 322, 589, 597, 888, 900
BseXI GCAGC 7 cut(s) 13, 60, 185, 682, 842, 982, 1004
BsgI GTGCAG 1 cut(s) 1461
BshFI GGCC 5 cut(s) 204, 899, 1079, 1191, 1469
BslFI GGGAC 3 cut(s) 922, 1097, 1392
BslI CCNNNNNNNGG 1 cut(s) 906
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 3 cut(s) 922, 1097, 1392
BsmI GAATGC 1 cut(s) 1012
BsnI GGCC 5 cut(s) 204, 899, 1079, 1191, 1469
Bsp1407I TGTACA 1 cut(s) 698
Bsp143I GATC 5 cut(s) 166, 431, 579, 840, 1345
Bsp1720I GCTNAGC 1 cut(s) 1407
Bsp19I CCATGG 2 cut(s) 357, 1320
BspACI CCGC 1 cut(s) 201
BspANI GGCC 5 cut(s) 204, 899, 1079, 1191, 1469
BspCNI CTCAG 3 cut(s) 995, 1399, 1440
BspLI GGNNCC 2 cut(s) 910, 911
BspMI ACCTGC 1 cut(s) 1459
BspPI GGATC 1 cut(s) 587
BsrDI GCAATG 2 cut(s) 36, 950
BsrGI TGTACA 1 cut(s) 698
BsrI ACTGG 5 cut(s) 322, 589, 597, 888, 900
BssECI CCNNGG 3 cut(s) 357, 956, 1320
BssMI GATC 5 cut(s) 166, 431, 579, 840, 1345
BssNAI GTATAC 1 cut(s) 478
BssT1I CCWWGG 3 cut(s) 357, 956, 1320
Bst1107I GTATAC 1 cut(s) 478
Bst2UI CCWGG 2 cut(s) 23, 1471
Bst4CI ACNGT 3 cut(s) 422, 743, 938
Bst6I CTCTTC 1 cut(s) 938
BstAUI TGTACA 1 cut(s) 698
BstC8I GCNNGC 2 cut(s) 380, 1413
BstDEI CTNAG 4 cut(s) 717, 982, 1407, 1427
BstDSI CCRYGG 2 cut(s) 357, 1320
BstF5I GGATG 3 cut(s) 181, 326, 701
BstKTI GATC 5 cut(s) 169, 434, 582, 843, 1348
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 5 cut(s) 166, 431, 579, 840, 1345
BstMWI GCNNNNNNNGC 2 cut(s) 1001, 1412
BstNI CCWGG 2 cut(s) 23, 1471
BstSCI CCNGG 2 cut(s) 21, 1469
BstV1I GCAGC 7 cut(s) 13, 60, 185, 682, 842, 982, 1004
BstV2I GAAGAC 2 cut(s) 1038, 1293
BstZ17I GTATAC 1 cut(s) 478
BsuI GTATCC 1 cut(s) 955
BsuRI GGCC 5 cut(s) 204, 899, 1079, 1191, 1469
BtgI CCRYGG 2 cut(s) 357, 1320
BtgZI GCGATG 1 cut(s) 1224
BtsCI GGATG 3 cut(s) 181, 326, 701
BtsIMutI CAGTG 3 cut(s) 582, 604, 943
BveI ACCTGC 1 cut(s) 1459
Cac8I GCNNGC 2 cut(s) 380, 1413
CaiI CAGNNNCTG 2 cut(s) 995, 1406
Cfr13I GGNCC 4 cut(s) 19, 767, 909, 1097
Csp6I GTAC 4 cut(s) 232, 321, 437, 699
CviAII CATG 9 cut(s) 283, 301, 358, 464, 484, 1140, 1297, 1307, 1321
CviQI GTAC 4 cut(s) 232, 321, 437, 699
DdeI CTNAG 4 cut(s) 717, 982, 1407, 1427
DpnI GATC 5 cut(s) 168, 433, 581, 842, 1347
DpnII GATC 5 cut(s) 166, 431, 579, 840, 1345
DraIII CACNNNGTG 1 cut(s) 311
DrdI GACNNNNNNGTC 1 cut(s) 1284
DseDI GACNNNNNNGTC 1 cut(s) 1284
EaeI YGGCCR 3 cut(s) 202, 1077, 1467
Eam1104I CTCTTC 1 cut(s) 938
EarI CTCTTC 1 cut(s) 938
Eco130I CCWWGG 3 cut(s) 357, 956, 1320
Eco47I GGWCC 4 cut(s) 19, 767, 909, 1097
Eco57I CTGAAG 1 cut(s) 891
EcoO109I RGGNCCY 1 cut(s) 909
EcoRII CCWGG 2 cut(s) 21, 1469
EcoT14I CCWWGG 3 cut(s) 357, 956, 1320
ErhI CCWWGG 3 cut(s) 357, 956, 1320
FaeI CATG 9 cut(s) 286, 304, 361, 467, 487, 1143, 1300, 1310, 1324
FaqI GGGAC 3 cut(s) 922, 1097, 1392
FatI CATG 9 cut(s) 282, 300, 357, 463, 483, 1139, 1296, 1306, 1320
FbaI TGATCA 3 cut(s) 166, 840, 1345
FblI GTMKAC 2 cut(s) 477, 1276
Fnu4HI GCNGC 8 cut(s) 27, 49, 199, 202, 671, 856, 993, 996
FokI GGATG 3 cut(s) 188, 313, 708
Fsp4HI GCNGC 8 cut(s) 27, 49, 199, 202, 671, 856, 993, 996
FspBI CTAG 6 cut(s) 404, 542, 725, 1116, 1157, 1166
GluI GCNGC 8 cut(s) 27, 49, 199, 202, 671, 856, 993, 996
GsuI CTGGAG 1 cut(s) 884
HaeIII GGCC 5 cut(s) 204, 899, 1079, 1191, 1469
Hin1II CATG 9 cut(s) 286, 304, 361, 467, 487, 1143, 1300, 1310, 1324
HincII GTYRAC 1 cut(s) 1062
HindII GTYRAC 1 cut(s) 1062
HinfI GANTC 4 cut(s) 783, 986, 1124, 1293
HpaI GTTAAC 1 cut(s) 1062
Hpy166II GTNNAC 7 cut(s) 297, 323, 443, 478, 1062, 1216, 1277
Hpy188I TCNGA 5 cut(s) 904, 985, 1149, 1350, 1397
Hpy188III TCNNGA 5 cut(s) 170, 643, 770, 863, 1166
Hpy8I GTNNAC 7 cut(s) 297, 323, 443, 478, 1062, 1216, 1277
HpyAV CCTTC 4 cut(s) 515, 598, 977, 1110
HpyCH4III ACNGT 3 cut(s) 422, 743, 938
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 7 cut(s) 7, 29, 80, 1012, 1019, 1415, 1442
HpyF10VI GCNNNNNNNGC 2 cut(s) 1001, 1412
HpyF3I CTNAG 4 cut(s) 717, 982, 1407, 1427
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 9 cut(s) 286, 304, 361, 467, 487, 1143, 1300, 1310, 1324
KflI GGGWCCC 1 cut(s) 909
Ksp22I TGATCA 3 cut(s) 166, 840, 1345
KspAI GTTAAC 1 cut(s) 1062
Kzo9I GATC 5 cut(s) 166, 431, 579, 840, 1345
LmnI GCTCC 2 cut(s) 721, 880
Lsp1109I GCAGC 7 cut(s) 13, 60, 185, 682, 842, 982, 1004
LweI GCATC 3 cut(s) 234, 1028, 1412
MaeI CTAG 6 cut(s) 404, 542, 725, 1116, 1157, 1166
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 6 cut(s) 58, 313, 755, 1246, 1326, 1359
MalI GATC 5 cut(s) 168, 433, 581, 842, 1347
MboI GATC 5 cut(s) 166, 431, 579, 840, 1345
MboII GAAGA 6 cut(s) 631, 785, 955, 1038, 1293, 1472
MfeI CAATTG 1 cut(s) 1193
MlsI TGGCCA 2 cut(s) 1079, 1469
MluCI AATT 4 cut(s) 470, 532, 705, 1193
MluNI TGGCCA 2 cut(s) 1079, 1469
MlyI GAGTC 1 cut(s) 792
MmeI TCCRAC 2 cut(s) 93, 1074
Mox20I TGGCCA 2 cut(s) 1079, 1469
MroXI GAANNNNTTC 1 cut(s) 522
MscI TGGCCA 2 cut(s) 1079, 1469
MseI TTAA 4 cut(s) 137, 621, 678, 1061
MslI CAYNNNNRTG 4 cut(s) 420, 678, 834, 936
Msp20I TGGCCA 2 cut(s) 1079, 1469
MspA1I CMGCKG 1 cut(s) 995
MspR9I CCNGG 2 cut(s) 23, 1471
MunI CAATTG 1 cut(s) 1193
Mva1269I GAATGC 1 cut(s) 1012
MvaI CCWGG 2 cut(s) 23, 1471
MwoI GCNNNNNNNGC 2 cut(s) 1001, 1412
NcoI CCATGG 2 cut(s) 357, 1320
NdeII GATC 5 cut(s) 166, 431, 579, 840, 1345
NlaIII CATG 9 cut(s) 286, 304, 361, 467, 487, 1143, 1300, 1310, 1324
NlaIV GGNNCC 2 cut(s) 910, 911
NmuCI GTSAC 2 cut(s) 755, 1359
OliI CACNNNNGTG 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 436
PctI GAATGC 1 cut(s) 1012
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 3 cut(s) 986, 1124, 1293
PkrI GCNGC 8 cut(s) 28, 50, 200, 203, 672, 857, 994, 997
PleI GAGTC 1 cut(s) 791
PpsI GAGTC 1 cut(s) 791
PpuMI RGGWCCY 1 cut(s) 909
PshBI ATTAAT 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 2 cut(s) 21, 1469
PspGI CCWGG 2 cut(s) 21, 1469
PspN4I GGNNCC 2 cut(s) 910, 911
PspPI GGNCC 4 cut(s) 19, 767, 909, 1097
PspPPI RGGWCCY 1 cut(s) 909
PstNI CAGNNNCTG 2 cut(s) 995, 1406
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 4 cut(s) 233, 322, 438, 700
RsaNI GTAC 4 cut(s) 232, 321, 437, 699
RseI CAYNNNNRTG 4 cut(s) 420, 678, 834, 936
SaqAI TTAA 4 cut(s) 137, 621, 678, 1061
SatI GCNGC 8 cut(s) 27, 49, 199, 202, 671, 856, 993, 996
Sau3AI GATC 5 cut(s) 166, 431, 579, 840, 1345
Sau96I GGNCC 4 cut(s) 19, 767, 909, 1097
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 1 cut(s) 792
ScrFI CCNGG 2 cut(s) 23, 1471
SfaNI GCATC 3 cut(s) 234, 1028, 1412
SinI GGWCC 4 cut(s) 19, 767, 909, 1097
SmiMI CAYNNNNRTG 4 cut(s) 420, 678, 834, 936
Sse9I AATT 4 cut(s) 470, 532, 705, 1193
SsiI CCGC 1 cut(s) 201
SspMI CTAG 6 cut(s) 404, 542, 725, 1116, 1157, 1166
StyD4I CCNGG 2 cut(s) 21, 1469
StyI CCWWGG 3 cut(s) 357, 956, 1320
TaaI ACNGT 3 cut(s) 422, 743, 938
TaiI ACGT 1 cut(s) 442
TaqI TCGA 4 cut(s) 228, 450, 786, 1103
TasI AATT 4 cut(s) 470, 532, 705, 1193
TatI WGTACW 2 cut(s) 231, 698
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 3 cut(s) 986, 1124, 1293
Tru1I TTAA 4 cut(s) 137, 621, 678, 1061
Tru9I TTAA 4 cut(s) 137, 621, 678, 1061
TscAI CASTG 3 cut(s) 589, 604, 943
TseFI GTSAC 2 cut(s) 755, 1359
TseI GCWGC 7 cut(s) 26, 48, 198, 670, 855, 992, 995
Tsp45I GTSAC 2 cut(s) 755, 1359
TspRI CASTG 3 cut(s) 589, 604, 943
VpaK11BI GGWCC 4 cut(s) 19, 767, 909, 1097
VspI ATTAAT 1 cut(s) 678
XapI RAATTY 1 cut(s) 532
XbaI TCTAGA 1 cut(s) 1165
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XmiI GTMKAC 2 cut(s) 477, 1276
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 6 cut(s) 404, 542, 725, 1116, 1157, 1166
ZrmI AGTACT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.