Rh5AG312600

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001114
Physical Location & Seq
Forward (+)
45194599 .. 45204886
10288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG312600.1

Sequence Viewer

Length: 1983 bp
ATGGAGAGCTTGATTCTGGTACCAGAGCTTCTCTTGCCCAACAGGATCTTTCGGTTAACCTTATTAGTTTCTTTGTTGTTCTTAGCAATTCCATTAGAAGCAGCCAACGCCTACCCAAAAAAATGTGTAAATCTCAACCTCACATTGCCCTTTGAATTTGGCAGTCCCGTAAAAGGAAGTGGATCTTCTATAGGTGATTACTGGTTTGACATAAGACATAGCAGCACTTCCTATAGAACACCAACATCCTTCATAGGCGAAACTAGTCTTGAGGTACAGGTACAGGTTTCTTCATTTACCAAGGTACGCGTAAATAACTCCATTGTTTACGCTTATAGTAATTATAGGTGCTACACTGAAGCTGGAGCGAAGATGTTGCACGCAAGCAATCTCCATGCTGCTGCAATCAATTTGACAGATGGCGCCCAGATTATTAGAACCTCCAGCACTATTACTGCAAACAAGTTCACATTGATAGGTTATGAGTCATTTGATTTTGTAGGTTATGACCCATTAATTTCCAGCTTCAACAGTACAATGATTAATCAGGAAAGAAGGGCTTGTTCTGGTATTAACTGTGGTCATTCTTATATTCAAAGAGGCATCAAGTCCTTTTTTCTGAAAGAGGCAGGAAGTGTAGGAAACATTACTTTGTTAGCTATGTATTTTTGGTTTATCATGAGACTGAGTGGTGGTAGTGGTACACCAATCAAGTCCAGACTCACGCTTCCTGTGTTTATGGTAGAAAACATCAGCTATGCGAGTCGTCACACTCTACGGCCACCAGGCAGCTCCAGGTTGAAATTATCAGCTCACACTCTCTATGGCATTCCAATTCAAAAAAGGGATTCCTTGTATCTAAGGTTCGCGATCAAAGTTGTCACACATAAAAATCTGGAAAACCACATGAAAAAGGACAGAAGTTTCATTGCACACATCAAAGTATTTTGGGAAAATTTCATTCTAGGTCTTGGCTTTTGTTGCTTGTTTCTTGTGTTTGGGATCACTGGGATATATTCCAGTGTGAAGAAACAAAAGTCGGCTAAGCTCAAATCCGAGTTCTTCCAGAAAAATGGAGGGTTATTGTTAGAGCAGCGTATTAGTGCTTCAACCGGAGGATGTGCAATCATTTTCACATCCAAGGAGATAGAAATGGCTATCGACTATCATACGAAGAGTCACATTTTTGGTCCAGGAGAGGGTGGAGTCGACCGTTTCATAGATGAGATTATCACTTTTACCCAAATCAACCATCAAAATATGATCAAGTTCTTCGGCTGCTGTCTAGAGACTGAAGCACCCATACTGGTCTATGAACTTGCCTCCAATGGGACCCTTTTCGACTACATTCATCACACCAATGGGAAAAGATCATTGTCTTGGGATACGCTTCTAAAGATAGCCTCAGAATCTGCAGCAGGTCTCAATTATCTGCATTCTGCATCAATCATTCATGGAAATGTAAAGTCTTCCAACGTTTTACTAACTGATCGTTTCATTGCCAAAGTTGCCGGATTTGGACCTCCGAGGTTATTTCCTACTAATGAATCCCAGATAAGCACACTGCTGCAGAGGACACTAGGCTATCTAGACCCGGAGTATCTTCATAAAGGTCAACTCACATATAAGAGTGATGTCTACAGCTTTGGAATGGTTTTATTAGAGATTGTAACAGGAGAGAAGCCAATATCATTTGGTAGACCAGAAAGTCAGAGAATCATATCTTCCCATTTTATTTCATCCATGGAATTACAAAGTGGTCTCTTTCATGAGATTGTTTGGCTACAAATTGTAAGAGAAGGAGACAGAGAACAAGTCAGAGCAGTAGCAGAGCTTGCAAAAAGGTGCCTCAATCTGAGTAGTGCAGAAAGACCAACAATGGAAGAAGTGGCCGGAGAGCTAAAGAGGCTGTCTGACAGCCACCAAGTACAAGTTGGCCGCCATGACAACAAAAGCTATTTGGAGATGAACCAGCCGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

660

Amino Acids

73.89

Weight (kDa)

8.98

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 404 - 633 3.7e-36 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 404 - 630 3.4e-31 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1707
Acc36I ACCTGC 1 cut(s) 1409
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 3 cut(s) 19, 422, 1845
AccI GTMKAC 3 cut(s) 1209, 1638, 1699
AccII CGCG 2 cut(s) 309, 869
AciI CCGC 1 cut(s) 1939
AclI AACGTT 1 cut(s) 1476
AclWI GGATC 3 cut(s) 53, 190, 1010
AcoI YGGCCR 3 cut(s) 779, 1890, 1936
AcsI RAATTY 2 cut(s) 155, 955
AcuI CTGAAG 2 cut(s) 378, 1314
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 7 cut(s) 21, 276, 282, 306, 535, 703, 1929
AfiI CCNNNNNNNGG 3 cut(s) 173, 1199, 1329
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 6 cut(s) 155, 529, 596, 802, 839, 1110
AhlI ACTAGT 1 cut(s) 263
AjnI CCWGG 3 cut(s) 784, 794, 1192
Alw26I GTCTC 5 cut(s) 676, 1283, 1427, 1766, 1797
AlwI GGATC 3 cut(s) 53, 190, 1010
AlwNI CAGNNNCTG 1 cut(s) 1412
AoxI GGCC 3 cut(s) 779, 1890, 1936
ApeKI GCWGC 9 cut(s) 101, 222, 398, 401, 789, 1093, 1278, 1415, 1567
ApoI RAATTY 2 cut(s) 155, 955
AseI ATTAAT 2 cut(s) 515, 543
Asp718I GGTACC 1 cut(s) 19
AspLEI GCGC 1 cut(s) 425
AspS9I GGNCC 3 cut(s) 1190, 1332, 1520
AsuC2I CCSGG 1 cut(s) 1595
AsuHPI GGTGA 1 cut(s) 206
AvaII GGWCC 3 cut(s) 1190, 1332, 1520
BanI GGYRCC 3 cut(s) 19, 422, 1845
BarI GAAGNNNNNNTAC 6 cut(s) 12, 44, 1090, 1122, 1287, 1319
BbsI GAAGAC 1 cut(s) 1461
BbvI GCAGC 9 cut(s) 113, 234, 385, 388, 801, 1105, 1265, 1427, 1554
BccI CCATC 2 cut(s) 413, 1260
BceAI ACGGC 1 cut(s) 794
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 3 cut(s) 786, 796, 1194
BciVI GTATCC 1 cut(s) 1378
BclI TGATCA 1 cut(s) 1263
BcnI CCSGG 1 cut(s) 1595
BcoDI GTCTC 5 cut(s) 676, 1283, 1427, 1766, 1797
BcuI ACTAGT 1 cut(s) 263
BfaI CTAG 5 cut(s) 264, 965, 1286, 1580, 1589
BfmI CTRYAG 5 cut(s) 189, 232, 1413, 1568, 1639
BfoI RGCGCY 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 1409
BfuI GTATCC 1 cut(s) 1378
BlpI GCTNAGC 1 cut(s) 1044
Bme1390I CCNGG 4 cut(s) 786, 796, 1194, 1595
Bme18I GGWCC 3 cut(s) 1190, 1332, 1520
BmgT120I GGNCC 3 cut(s) 1190, 1332, 1520
BmiI GGNNCC 5 cut(s) 21, 424, 1333, 1334, 1847
BmrFI CCNGG 4 cut(s) 786, 796, 1194, 1595
BmrI ACTGGG 1 cut(s) 1017
BmsI GCATC 2 cut(s) 612, 1451
BmuI ACTGGG 1 cut(s) 1017
BpiI GAAGAC 1 cut(s) 1461
BpmI CTGGAG 3 cut(s) 384, 427, 778
Bpu1102I GCTNAGC 1 cut(s) 1044
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 1595
BsaHI GRCGYC 1 cut(s) 423
BsaI GGTCTC 2 cut(s) 1427, 1766
BsaJI CCNNGG 4 cut(s) 300, 1140, 1526, 1743
BsaWI WCCGGW 1 cut(s) 1112
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 1199, 1329
Bse1I ACTGG 4 cut(s) 206, 1012, 1020, 1311
Bse3DI GCAATG 3 cut(s) 143, 927, 1497
BseBI CCWGG 3 cut(s) 786, 796, 1194
BseDI CCNNGG 4 cut(s) 300, 1140, 1526, 1743
BseGI GGATG 4 cut(s) 245, 1124, 1136, 1739
BseLI CCNNNNNNNGG 3 cut(s) 173, 1199, 1329
BseMI GCAATG 3 cut(s) 143, 927, 1497
BseMII CTCAG 3 cut(s) 677, 1419, 1847
BseNI ACTGG 4 cut(s) 206, 1012, 1020, 1311
BseXI GCAGC 9 cut(s) 113, 234, 385, 388, 801, 1105, 1265, 1427, 1554
BsgI GTGCAG 1 cut(s) 1884
Bsh1236I CGCG 2 cut(s) 309, 869
Bsh1285I CGRYCG 1 cut(s) 1213
BshFI GGCC 3 cut(s) 781, 1892, 1938
BshNI GGYRCC 3 cut(s) 19, 422, 1845
BsiEI CGRYCG 1 cut(s) 1213
BsiSI CCGG 5 cut(s) 1113, 1512, 1595, 1893, 1976
BslFI GGGAC 2 cut(s) 150, 1345
BslI CCNNNNNNNGG 3 cut(s) 173, 1199, 1329
BsmAI GTCTC 5 cut(s) 676, 1283, 1427, 1766, 1797
BsmFI GGGAC 2 cut(s) 150, 1345
BsmI GAATGC 2 cut(s) 828, 1435
BsnI GGCC 3 cut(s) 781, 1892, 1938
Bso31I GGTCTC 2 cut(s) 1427, 1766
Bsp143I GATC 7 cut(s) 45, 182, 870, 1002, 1263, 1370, 1489
Bsp1720I GCTNAGC 1 cut(s) 1044
Bsp19I CCATGG 1 cut(s) 1743
Bsp68I TCGCGA 1 cut(s) 869
BspACI CCGC 1 cut(s) 1939
BspANI GGCC 3 cut(s) 781, 1892, 1938
BspCNI CTCAG 3 cut(s) 678, 1418, 1848
BspFNI CGCG 2 cut(s) 309, 869
BspHI TCATGA 2 cut(s) 678, 1768
BspLI GGNNCC 5 cut(s) 21, 424, 1333, 1334, 1847
BspMAI CTGCAG 2 cut(s) 1417, 1572
BspMI ACCTGC 1 cut(s) 1409
BspPI GGATC 3 cut(s) 53, 190, 1010
BspT107I GGYRCC 3 cut(s) 19, 422, 1845
BspTNI GGTCTC 2 cut(s) 1427, 1766
BsrDI GCAATG 3 cut(s) 143, 927, 1497
BsrI ACTGG 4 cut(s) 206, 1012, 1020, 1311
BssECI CCNNGG 4 cut(s) 300, 1140, 1526, 1743
BssMI GATC 7 cut(s) 45, 182, 870, 1002, 1263, 1370, 1489
BssNI GRCGYC 1 cut(s) 423
BssT1I CCWWGG 3 cut(s) 300, 1140, 1743
Bst2UI CCWGG 3 cut(s) 786, 796, 1194
Bst4CI ACNGT 3 cut(s) 533, 578, 1214
Bst6I CTCTTC 1 cut(s) 1169
BstACI GRCGYC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 1835
BstC8I GCNNGC 3 cut(s) 381, 385, 1836
BstDEI CTNAG 6 cut(s) 82, 686, 860, 1044, 1405, 1856
BstDSI CCRYGG 1 cut(s) 1743
BstF5I GGATG 4 cut(s) 245, 1124, 1136, 1739
BstFNI CGCG 2 cut(s) 309, 869
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 7 cut(s) 48, 185, 873, 1005, 1266, 1373, 1492
BstMAI GTCTC 5 cut(s) 676, 1283, 1427, 1766, 1797
BstMBI GATC 7 cut(s) 45, 182, 870, 1002, 1263, 1370, 1489
BstMCI CGRYCG 1 cut(s) 1213
BstMWI GCNNNNNNNGC 6 cut(s) 34, 107, 981, 1508, 1835, 1906
BstNI CCWGG 3 cut(s) 786, 796, 1194
BstSCI CCNGG 4 cut(s) 784, 794, 1192, 1593
BstSFI CTRYAG 5 cut(s) 189, 232, 1413, 1568, 1639
BstUI CGCG 2 cut(s) 309, 869
BstV1I GCAGC 9 cut(s) 113, 234, 385, 388, 801, 1105, 1265, 1427, 1554
BstV2I GAAGAC 1 cut(s) 1461
BstX2I RGATCY 2 cut(s) 45, 182
BstXI CCANNNNNNTGG 1 cut(s) 1073
BstYI RGATCY 2 cut(s) 45, 182
BsuI GTATCC 1 cut(s) 1378
BsuRI GGCC 3 cut(s) 781, 1892, 1938
BtgI CCRYGG 1 cut(s) 1743
BtsCI GGATG 4 cut(s) 245, 1124, 1136, 1739
BtsI GCAGTG 1 cut(s) 1562
BtsIMutI CAGTG 4 cut(s) 354, 1005, 1027, 1562
BtuMI TCGCGA 1 cut(s) 869
BveI ACCTGC 1 cut(s) 1409
Cac8I GCNNGC 3 cut(s) 381, 385, 1836
CaiI CAGNNNCTG 1 cut(s) 1412
CciI TCATGA 2 cut(s) 678, 1768
CfoI GCGC 1 cut(s) 425
Cfr13I GGNCC 3 cut(s) 1190, 1332, 1520
Csp6I GTAC 7 cut(s) 20, 275, 281, 305, 534, 702, 1928
CviAII CATG 7 cut(s) 395, 679, 907, 1454, 1744, 1769, 1943
CviQI GTAC 7 cut(s) 20, 275, 281, 305, 534, 702, 1928
DdeI CTNAG 6 cut(s) 82, 686, 860, 1044, 1405, 1856
DinI GGCGCC 1 cut(s) 424
DpnI GATC 7 cut(s) 47, 184, 872, 1004, 1265, 1372, 1491
DpnII GATC 7 cut(s) 45, 182, 870, 1002, 1263, 1370, 1489
DrdI GACNNNNNNGTC 1 cut(s) 1707
DseDI GACNNNNNNGTC 1 cut(s) 1707
EaeI YGGCCR 3 cut(s) 779, 1890, 1936
Eam1104I CTCTTC 1 cut(s) 1169
EarI CTCTTC 1 cut(s) 1169
Eco130I CCWWGG 3 cut(s) 300, 1140, 1743
Eco31I GGTCTC 2 cut(s) 1427, 1766
Eco47I GGWCC 3 cut(s) 1190, 1332, 1520
Eco57I CTGAAG 2 cut(s) 378, 1314
EcoO109I RGGNCCY 1 cut(s) 1332
EcoRII CCWGG 3 cut(s) 784, 794, 1192
EcoT14I CCWWGG 3 cut(s) 300, 1140, 1743
EgeI GGCGCC 1 cut(s) 424
EheI GGCGCC 1 cut(s) 424
ErhI CCWWGG 3 cut(s) 300, 1140, 1743
FaeI CATG 7 cut(s) 398, 682, 910, 1457, 1747, 1772, 1946
FalI AAGNNNNNCTT 4 cut(s) 169, 201, 544, 576
FaqI GGGAC 2 cut(s) 150, 1345
FatI CATG 7 cut(s) 394, 678, 906, 1453, 1743, 1768, 1942
FbaI TGATCA 1 cut(s) 1263
FblI GTMKAC 3 cut(s) 1209, 1638, 1699
FokI GGATG 4 cut(s) 232, 1123, 1131, 1726
FspBI CTAG 5 cut(s) 264, 965, 1286, 1580, 1589
GlaI GCGC 1 cut(s) 424
GsuI CTGGAG 3 cut(s) 384, 427, 778
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 3 cut(s) 781, 1892, 1938
HapII CCGG 5 cut(s) 1113, 1512, 1595, 1893, 1976
HhaI GCGC 1 cut(s) 425
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 7 cut(s) 398, 682, 910, 1457, 1747, 1772, 1946
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HincII GTYRAC 3 cut(s) 57, 1210, 1616
HindII GTYRAC 3 cut(s) 57, 1210, 1616
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 5 cut(s) 1113, 1512, 1595, 1893, 1976
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 8 cut(s) 57, 328, 468, 704, 1210, 1616, 1639, 1700
Hpy188I TCNGA 8 cut(s) 621, 1057, 1408, 1527, 1713, 1820, 1857, 1915
Hpy8I GTNNAC 8 cut(s) 57, 328, 468, 704, 1210, 1616, 1639, 1700
HpyAV CCTTC 3 cut(s) 259, 549, 1793
HpyCH4III ACNGT 3 cut(s) 533, 578, 1214
HpyCH4IV ACGT 1 cut(s) 1476
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 107, 981, 1508, 1835, 1906
HpyF3I CTNAG 6 cut(s) 82, 686, 860, 1044, 1405, 1856
HpySE526I ACGT 1 cut(s) 1476
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 7 cut(s) 398, 682, 910, 1457, 1747, 1772, 1946
HspAI GCGC 1 cut(s) 423
KasI GGCGCC 1 cut(s) 422
KflI GGGWCCC 1 cut(s) 1332
KpnI GGTACC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 1263
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 7 cut(s) 45, 182, 870, 1002, 1263, 1370, 1489
LmnI GCTCC 2 cut(s) 365, 797
Lsp1109I GCAGC 9 cut(s) 113, 234, 385, 388, 801, 1105, 1265, 1427, 1554
LweI GCATC 2 cut(s) 612, 1451
MaeI CTAG 5 cut(s) 264, 965, 1286, 1580, 1589
MaeII ACGT 1 cut(s) 1476
MaeIII GTNAC 4 cut(s) 767, 880, 1178, 1669
MalI GATC 7 cut(s) 47, 184, 872, 1004, 1265, 1372, 1491
MboI GATC 7 cut(s) 45, 182, 870, 1002, 1263, 1370, 1489
MflI RGATCY 2 cut(s) 45, 182
MluI ACGCGT 1 cut(s) 307
Mly113I GGCGCC 1 cut(s) 423
MlyI GAGTC 5 cut(s) 494, 714, 772, 1186, 1215
MmeI TCCRAC 1 cut(s) 1497
MseI TTAA 4 cut(s) 56, 515, 543, 573
MslI CAYNNNNRTG 2 cut(s) 1359, 1458
MspI CCGG 5 cut(s) 1113, 1512, 1595, 1893, 1976
MspR9I CCNGG 4 cut(s) 786, 796, 1194, 1595
Mva1269I GAATGC 2 cut(s) 828, 1435
MvaI CCWGG 3 cut(s) 786, 796, 1194
MvnI CGCG 2 cut(s) 309, 869
MwoI GCNNNNNNNGC 6 cut(s) 34, 107, 981, 1508, 1835, 1906
NarI GGCGCC 1 cut(s) 423
NciI CCSGG 1 cut(s) 1595
NcoI CCATGG 1 cut(s) 1743
NdeII GATC 7 cut(s) 45, 182, 870, 1002, 1263, 1370, 1489
NlaIII CATG 7 cut(s) 398, 682, 910, 1457, 1747, 1772, 1946
NlaIV GGNNCC 5 cut(s) 21, 424, 1333, 1334, 1847
NmuCI GTSAC 3 cut(s) 767, 880, 1178
NruI TCGCGA 1 cut(s) 869
PagI TCATGA 2 cut(s) 678, 1768
PctI GAATGC 2 cut(s) 828, 1435
PfeI GAWTC 5 cut(s) 13, 848, 1409, 1547, 1716
PfoI TCCNGGA 1 cut(s) 1192
PleI GAGTC 5 cut(s) 493, 714, 771, 1185, 1214
PluTI GGCGCC 1 cut(s) 426
PpsI GAGTC 5 cut(s) 493, 714, 771, 1185, 1214
PpuMI RGGWCCY 1 cut(s) 1332
PshBI ATTAAT 2 cut(s) 515, 543
Psp1406I AACGTT 1 cut(s) 1476
Psp5II RGGWCCY 1 cut(s) 1332
Psp6I CCWGG 3 cut(s) 784, 794, 1192
PspGI CCWGG 3 cut(s) 784, 794, 1192
PspN4I GGNNCC 5 cut(s) 21, 424, 1333, 1334, 1847
PspPI GGNCC 3 cut(s) 1190, 1332, 1520
PspPPI RGGWCCY 1 cut(s) 1332
PsrI GAACNNNNNNTAC 2 cut(s) 848, 880
PstI CTGCAG 2 cut(s) 1417, 1572
PstNI CAGNNNCTG 1 cut(s) 1412
PsuI RGATCY 2 cut(s) 45, 182
RruI TCGCGA 1 cut(s) 869
RsaI GTAC 7 cut(s) 21, 276, 282, 306, 535, 703, 1929
RsaNI GTAC 7 cut(s) 20, 275, 281, 305, 534, 702, 1928
RseI CAYNNNNRTG 2 cut(s) 1359, 1458
SalI GTCGAC 1 cut(s) 1208
SaqAI TTAA 4 cut(s) 56, 515, 543, 573
Sau3AI GATC 7 cut(s) 45, 182, 870, 1002, 1263, 1370, 1489
Sau96I GGNCC 3 cut(s) 1190, 1332, 1520
SchI GAGTC 5 cut(s) 494, 714, 772, 1186, 1215
ScrFI CCNGG 4 cut(s) 786, 796, 1194, 1595
SfaNI GCATC 2 cut(s) 612, 1451
SfcI CTRYAG 5 cut(s) 189, 232, 1413, 1568, 1639
SfoI GGCGCC 1 cut(s) 424
SinI GGWCC 3 cut(s) 1190, 1332, 1520
SmiMI CAYNNNNRTG 2 cut(s) 1359, 1458
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SpeI ACTAGT 1 cut(s) 263
SsiI CCGC 1 cut(s) 1939
SspDI GGCGCC 1 cut(s) 422
SspMI CTAG 5 cut(s) 264, 965, 1286, 1580, 1589
StyD4I CCNGG 4 cut(s) 784, 794, 1192, 1593
StyI CCWWGG 3 cut(s) 300, 1140, 1743
TaaI ACNGT 3 cut(s) 533, 578, 1214
TaiI ACGT 1 cut(s) 1479
TaqI TCGA 3 cut(s) 1161, 1209, 1341
TatI WGTACW 2 cut(s) 533, 1927
TauI GCSGC 1 cut(s) 1941
TfiI GAWTC 5 cut(s) 13, 848, 1409, 1547, 1716
Tru1I TTAA 4 cut(s) 56, 515, 543, 573
Tru9I TTAA 4 cut(s) 56, 515, 543, 573
TscAI CASTG 4 cut(s) 361, 1012, 1027, 1569
TseFI GTSAC 3 cut(s) 767, 880, 1178
TseI GCWGC 9 cut(s) 101, 222, 398, 401, 789, 1093, 1278, 1415, 1567
Tsp45I GTSAC 3 cut(s) 767, 880, 1178
TspRI CASTG 4 cut(s) 361, 1012, 1027, 1569
VpaK11BI GGWCC 3 cut(s) 1190, 1332, 1520
VspI ATTAAT 2 cut(s) 515, 543
XapI RAATTY 2 cut(s) 155, 955
XbaI TCTAGA 2 cut(s) 1285, 1588
XcmI CCANNNNNNNNNTGG 1 cut(s) 1931
XmiI GTMKAC 3 cut(s) 1209, 1638, 1699
XspI CTAG 5 cut(s) 264, 965, 1286, 1580, 1589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.