MD01G1234700.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001114
Physical Location & Seq
Forward (+)
32233000 .. 32234693
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1234700.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGATTCTGAAACTTGGACATCATCTCCTGCGCAAACTCTTTTGGTTTAGCTTAATATCATTGTTTTTGTTGGCACCGCTACCGCTATCAGAAGCCAACTCTAACACCAGCTTCCGAACTGCGGGCGTACACTGGCCTGACATAATAAACTGTAACACTTCCTACAGGCCAACAAATCCAGGACCATTCTCACGAACAAGTAATCTCGATGGCGCCATTTCCTTTGCAAATCCTGATGGCATCTGCAGCGGCGACGCTTACTATAATGATCTGATCTTTCAAGTATATCTTGGGAACTTCGTTGGTTTAGCTGGAGACACAACGATTAGAGTGAATCGATCAAAATATTATGATCACTTTTCAAAAGCAAACCAGGCATATAATCATGACCATAGGAAAACTGGTAGACGCTACATCAAACACAAAAAAGTCATTTCGGTAAAGCTCACTCTAGGTCTTGGCTTTTTTTGCTTATTTATGTCGTTTGGGATCACTGGGGTTTATTCCAGTGTGAAGAAACGGAAGTTCATTAATCTCAAAGCCAAGTTCTTCCAGCAAAATGGAGGGTTATTGTTAGAGCAGCATATTGCTTCACATGGAAGCACAAAAGTTTTTACAGCCGAGGAGCTAGAGACGGCCGCTGCCTATTATAACCAGAGTCATAATTTTTGTCCAGGAGAAAGTGCATTAGATTGGAAAGGACTTTTGTCAGACGGTGCGATTGTAGACATGAGGAAAACAGTAGTGCACAAGGGCCAAATCGAGCGCTTTATCTACGAGATCGTCACTCTTACCAAAATTAACCATAAAAATGTGGTGAAGTTCTTGGGCTGCTGCTTAGAGACTGAAGCTCCCATGTTAGTGTATGAATTCCCTTGCAATGGGACCTTGTTCGACTACATTCATCATGCTAACGGCAAGAGTGCATTGCCTTGGCATGTCCTTTTAAAGATAGCCTCAGACTCTGCAACTGCTCTTGCCTATATGCATTCAGCAGTAGACTCGTTGAAACCAATGATCATTCATGGAAATGTCAACTCGTCCAACATACTTTTAACTGATTGTTTTGTGGCCAAAGTCTCCGGTTTTAGTCCTTCAAGGTTGGTCCCGGTCCCCGGTAATGAATCCCAGATGAAGACATTGGTGCAGCAAACACTTGGTTATCTTGATCCGGAATATCTCTATACAGGCCAGTTGACAGATAAGAGCGATGTTTATAGCTTTGGAATCGTATTGTTGGAGCTTCTAACTGGGGAGATGCCATTGTCCTTTCACAGACCAGAAAATCAAAGAATCATAACATCCCATTTCGTTTCTTCTGTAGGACAAAATGACATCTTTCAGATCGTTGTGCCACAACTTGTAAACGAGGGAAACAGAGAGCAACTCAGAGCAGTTGCGGAGCTTGCAAAGAGTTGCCTCAAGTTGAGTAGTGCCCAAAGGCCTGCGATGGAAGAAGTGGCAAGAGAATTAAGGAGGTTGTGTGGCAGCACACATTCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

502

Amino Acids

55.94

Weight (kDa)

8.9

Isoelectric Point (pI)

31.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 244 - 488 1.7e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 246 - 491 1.5e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 651
Acc16I TGCGCA 1 cut(s) 32
AccB1I GGYRCC 2 cut(s) 73, 212
AccI GTMKAC 3 cut(s) 406, 726, 999
AccIII TCCGGA 1 cut(s) 1171
AciI CCGC 6 cut(s) 77, 83, 122, 249, 639, 1400
AclWI GGATC 2 cut(s) 497, 1163
AcoI YGGCCR 2 cut(s) 636, 1071
AcsI RAATTY 1 cut(s) 869
AcuI CTGAAG 1 cut(s) 867
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 129
AfeI AGCGCT 1 cut(s) 767
AfiI CCNNNNNNNGG 3 cut(s) 121, 881, 1115
AgsI TTSAA 4 cut(s) 281, 363, 1009, 1098
AjnI CCWGG 3 cut(s) 178, 372, 673
AluBI AGCT 9 cut(s) 51, 111, 311, 445, 628, 851, 1221, 1243, 1405
AluI AGCT 9 cut(s) 51, 111, 311, 445, 628, 851, 1221, 1243, 1405
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 4 cut(s) 309, 626, 836, 1084
Alw44I GTGCAC 1 cut(s) 746
AlwI GGATC 2 cut(s) 497, 1163
AlwNI CAGNNNCTG 1 cut(s) 965
Aor13HI TCCGGA 1 cut(s) 1171
Aor51HI AGCGCT 1 cut(s) 767
AoxI GGCC 7 cut(s) 134, 167, 636, 754, 1071, 1189, 1442
ApaLI GTGCAC 1 cut(s) 746
ApeKI GCWGC 7 cut(s) 246, 580, 641, 831, 834, 1147, 1488
ApoI RAATTY 1 cut(s) 869
AseI ATTAAT 1 cut(s) 531
AspLEI GCGC 3 cut(s) 33, 215, 768
AspS9I GGNCC 5 cut(s) 182, 754, 885, 1105, 1111
AsuC2I CCSGG 2 cut(s) 1109, 1116
AsuHPI GGTGA 1 cut(s) 829
AvaII GGWCC 4 cut(s) 182, 885, 1105, 1111
BaeGI GKGCMC 2 cut(s) 750, 1438
BalI TGGCCA 1 cut(s) 1073
BanI GGYRCC 2 cut(s) 73, 212
BbsI GAAGAC 1 cut(s) 1142
Bbv12I GWGCWC 1 cut(s) 750
BbvI GCAGC 7 cut(s) 258, 592, 628, 818, 821, 1159, 1500
BccI CCATC 3 cut(s) 203, 230, 1444
BceAI ACGGC 2 cut(s) 651, 931
BcgI CGANNNNNNTGC 2 cut(s) 984, 1018
BciT130I CCWGG 3 cut(s) 180, 374, 675
BclI TGATCA 2 cut(s) 352, 1017
BcnI CCSGG 2 cut(s) 1109, 1116
BcoDI GTCTC 4 cut(s) 309, 626, 836, 1084
BfaI CTAG 2 cut(s) 452, 629
BfmI CTRYAG 3 cut(s) 163, 244, 1320
BfoI RGCGCY 2 cut(s) 216, 769
BisI GCNGC 9 cut(s) 247, 250, 581, 639, 642, 832, 835, 1148, 1489
BlsI GCNGC 9 cut(s) 248, 251, 582, 640, 643, 833, 836, 1149, 1490
Bme1390I CCNGG 5 cut(s) 180, 374, 675, 1109, 1116
Bme18I GGWCC 4 cut(s) 182, 885, 1105, 1111
BmgT120I GGNCC 5 cut(s) 182, 754, 885, 1105, 1111
BmiI GGNNCC 5 cut(s) 75, 214, 886, 1107, 1113
BmrFI CCNGG 5 cut(s) 180, 374, 675, 1109, 1116
BmrI ACTGGG 2 cut(s) 504, 1260
BmsI GCATC 2 cut(s) 249, 1248
BmuI ACTGGG 2 cut(s) 504, 1260
BoxI GACNNNNGTC 1 cut(s) 706
BpiI GAAGAC 1 cut(s) 1142
BplI GAGNNNNNCTC 2 cut(s) 1371, 1403
BpmI CTGGAG 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 1406
BpuMI CCSGG 2 cut(s) 1109, 1116
Bsa29I ATCGAT 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 621, 932, 1114
BsaWI WCCGGW 2 cut(s) 1082, 1171
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 835, 865, 1468, 1498
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 881, 1115
Bse1I ACTGG 6 cut(s) 137, 406, 499, 507, 1192, 1255
Bse3DI GCAATG 2 cut(s) 886, 926
BseAI TCCGGA 1 cut(s) 1171
BseBI CCWGG 3 cut(s) 180, 374, 675
BseCI ATCGAT 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 621, 932, 1114
BseGI GGATG 1 cut(s) 1301
BseLI CCNNNNNNNGG 3 cut(s) 121, 881, 1115
BseMI GCAATG 2 cut(s) 886, 926
BseMII CTCAG 2 cut(s) 972, 1402
BseNI ACTGG 6 cut(s) 137, 406, 499, 507, 1192, 1255
BseRI GAGGAG 1 cut(s) 638
BseSI GKGCMC 2 cut(s) 750, 1438
BseX3I CGGCCG 1 cut(s) 636
BseXI GCAGC 7 cut(s) 258, 592, 628, 818, 821, 1159, 1500
BsgI GTGCAG 1 cut(s) 1166
Bsh1285I CGRYCG 1 cut(s) 639
BshFI GGCC 7 cut(s) 136, 169, 638, 756, 1073, 1191, 1444
BshNI GGYRCC 2 cut(s) 73, 212
BshVI ATCGAT 1 cut(s) 337
BsiEI CGRYCG 1 cut(s) 639
BsiHKAI GWGCWC 1 cut(s) 750
BsiSI CCGG 4 cut(s) 1083, 1109, 1116, 1172
BslFI GGGAC 3 cut(s) 898, 1091, 1097
BslI CCNNNNNNNGG 3 cut(s) 121, 881, 1115
BsmAI GTCTC 4 cut(s) 309, 626, 836, 1084
BsmBI CGTCTC 1 cut(s) 626
BsmFI GGGAC 3 cut(s) 898, 1091, 1097
BsmI GAATGC 1 cut(s) 988
BsnI GGCC 7 cut(s) 136, 169, 638, 756, 1073, 1191, 1444
Bsp1286I GDGCHC 2 cut(s) 750, 1438
Bsp13I TCCGGA 1 cut(s) 1171
Bsp143I GATC 9 cut(s) 268, 273, 338, 352, 489, 780, 1017, 1168, 1344
BspACI CCGC 6 cut(s) 77, 83, 122, 249, 639, 1400
BspANI GGCC 7 cut(s) 136, 169, 638, 756, 1073, 1191, 1444
BspCNI CTCAG 2 cut(s) 971, 1401
BspDI ATCGAT 1 cut(s) 337
BspEI TCCGGA 1 cut(s) 1171
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 5 cut(s) 75, 214, 886, 1107, 1113
BspMAI CTGCAG 1 cut(s) 248
BspPI GGATC 2 cut(s) 497, 1163
BspT107I GGYRCC 2 cut(s) 73, 212
BsrDI GCAATG 2 cut(s) 886, 926
BsrI ACTGG 6 cut(s) 137, 406, 499, 507, 1192, 1255
BssECI CCNNGG 3 cut(s) 621, 932, 1114
BssMI GATC 9 cut(s) 268, 273, 338, 352, 489, 780, 1017, 1168, 1344
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 932
Bst2UI CCWGG 3 cut(s) 180, 374, 675
Bst4CI ACNGT 3 cut(s) 152, 716, 742
BstACI GRCGYC 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 124, 1407, 1446
BstDEI CTNAG 3 cut(s) 838, 958, 1388
BstF5I GGATG 1 cut(s) 1301
BstH2I RGCGCY 2 cut(s) 216, 769
BstHHI GCGC 3 cut(s) 33, 215, 768
BstKTI GATC 9 cut(s) 271, 276, 341, 355, 492, 783, 1020, 1171, 1347
BstMAI GTCTC 4 cut(s) 309, 626, 836, 1084
BstMBI GATC 9 cut(s) 268, 273, 338, 352, 489, 780, 1017, 1168, 1344
BstMCI CGRYCG 1 cut(s) 639
BstMWI GCNNNNNNNGC 4 cut(s) 246, 374, 468, 1406
BstNI CCWGG 3 cut(s) 180, 374, 675
BstNSI RCATGY 1 cut(s) 941
BstPAI GACNNNNGTC 1 cut(s) 706
BstSCI CCNGG 5 cut(s) 178, 372, 673, 1107, 1114
BstSFI CTRYAG 3 cut(s) 163, 244, 1320
BstSLI GKGCMC 2 cut(s) 750, 1438
BstV1I GCAGC 7 cut(s) 258, 592, 628, 818, 821, 1159, 1500
BstV2I GAAGAC 1 cut(s) 1142
BstXI CCANNNNNNTGG 1 cut(s) 560
BstZI CGGCCG 1 cut(s) 636
Bsu15I ATCGAT 1 cut(s) 337
BsuRI GGCC 7 cut(s) 136, 169, 638, 756, 1073, 1191, 1444
BsuTUI ATCGAT 1 cut(s) 337
BtgZI GCGATG 2 cut(s) 1224, 1463
BtsCI GGATG 1 cut(s) 1301
BtsIMutI CAGTG 3 cut(s) 130, 492, 514
Cac8I GCNNGC 3 cut(s) 124, 1407, 1446
CaiI CAGNNNCTG 1 cut(s) 965
CciI TCATGA 1 cut(s) 385
CfoI GCGC 3 cut(s) 33, 215, 768
Cfr13I GGNCC 5 cut(s) 182, 754, 885, 1105, 1111
ClaI ATCGAT 1 cut(s) 337
CseI GACGC 2 cut(s) 263, 417
Csp6I GTAC 1 cut(s) 128
CviAII CATG 7 cut(s) 386, 596, 730, 856, 908, 938, 1025
CviQI GTAC 1 cut(s) 128
DdeI CTNAG 3 cut(s) 838, 958, 1388
DinI GGCGCC 1 cut(s) 214
DpnI GATC 9 cut(s) 270, 275, 340, 354, 491, 782, 1019, 1170, 1346
DpnII GATC 9 cut(s) 268, 273, 338, 352, 489, 780, 1017, 1168, 1344
DraI TTTAAA 1 cut(s) 948
EaeI YGGCCR 2 cut(s) 636, 1071
EagI CGGCCG 1 cut(s) 636
EclXI CGGCCG 1 cut(s) 636
Eco130I CCWWGG 1 cut(s) 932
Eco147I AGGCCT 1 cut(s) 1444
Eco47I GGWCC 4 cut(s) 182, 885, 1105, 1111
Eco47III AGCGCT 1 cut(s) 767
Eco52I CGGCCG 1 cut(s) 636
Eco57I CTGAAG 1 cut(s) 867
EcoO109I RGGNCCY 1 cut(s) 885
EcoRI GAATTC 1 cut(s) 869
EcoRII CCWGG 3 cut(s) 178, 372, 673
EcoT14I CCWWGG 1 cut(s) 932
EcoT22I ATGCAT 1 cut(s) 990
EgeI GGCGCC 1 cut(s) 214
EheI GGCGCC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 932
Esp3I CGTCTC 1 cut(s) 626
FaeI CATG 7 cut(s) 389, 599, 733, 859, 911, 941, 1028
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FaqI GGGAC 3 cut(s) 898, 1091, 1097
FatI CATG 7 cut(s) 385, 595, 729, 855, 907, 937, 1024
FauI CCCGC 1 cut(s) 115
FbaI TGATCA 2 cut(s) 352, 1017
FblI GTMKAC 3 cut(s) 406, 726, 999
Fnu4HI GCNGC 9 cut(s) 247, 250, 581, 639, 642, 832, 835, 1148, 1489
FokI GGATG 1 cut(s) 1288
Fsp4HI GCNGC 9 cut(s) 247, 250, 581, 639, 642, 832, 835, 1148, 1489
FspBI CTAG 2 cut(s) 452, 629
FspI TGCGCA 1 cut(s) 32
GlaI GCGC 3 cut(s) 32, 214, 767
GluI GCNGC 9 cut(s) 247, 250, 581, 639, 642, 832, 835, 1148, 1489
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 2 cut(s) 216, 769
HaeIII GGCC 7 cut(s) 136, 169, 638, 756, 1073, 1191, 1444
HapII CCGG 4 cut(s) 1083, 1109, 1116, 1172
HgaI GACGC 2 cut(s) 263, 417
HhaI GCGC 3 cut(s) 33, 215, 768
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 7 cut(s) 389, 599, 733, 859, 911, 941, 1028
Hin6I GCGC 3 cut(s) 31, 213, 766
HinP1I GCGC 3 cut(s) 31, 213, 766
HincII GTYRAC 2 cut(s) 1036, 1197
HindII GTYRAC 2 cut(s) 1036, 1197
HinfI GANTC 8 cut(s) 4, 334, 658, 962, 1001, 1124, 1227, 1293
HpaII CCGG 4 cut(s) 1083, 1109, 1116, 1172
HphI GGTGA 1 cut(s) 829
Hpy166II GTNNAC 8 cut(s) 130, 407, 727, 748, 1000, 1036, 1197, 1366
Hpy188I TCNGA 8 cut(s) 9, 91, 116, 273, 712, 961, 1344, 1391
Hpy188III TCNNGA 6 cut(s) 192, 206, 233, 386, 1166, 1172
Hpy8I GTNNAC 8 cut(s) 130, 407, 727, 748, 1000, 1036, 1197, 1366
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 1 cut(s) 1104
HpyCH4III ACNGT 3 cut(s) 152, 716, 742
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 374, 468, 1406
HpyF3I CTNAG 3 cut(s) 838, 958, 1388
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 7 cut(s) 389, 599, 733, 859, 911, 941, 1028
HspAI GCGC 3 cut(s) 31, 213, 766
KasI GGCGCC 1 cut(s) 212
Kpn2I TCCGGA 1 cut(s) 1171
Ksp22I TGATCA 2 cut(s) 352, 1017
Kzo9I GATC 9 cut(s) 268, 273, 338, 352, 489, 780, 1017, 1168, 1344
LmnI GCTCC 4 cut(s) 625, 856, 1240, 1402
Lsp1109I GCAGC 7 cut(s) 258, 592, 628, 818, 821, 1159, 1500
LweI GCATC 2 cut(s) 249, 1248
MaeI CTAG 2 cut(s) 452, 629
MaeIII GTNAC 2 cut(s) 152, 784
MalI GATC 9 cut(s) 270, 275, 340, 354, 491, 782, 1019, 1170, 1346
MboI GATC 9 cut(s) 268, 273, 338, 352, 489, 780, 1017, 1168, 1344
MboII GAAGA 6 cut(s) 526, 541, 1147, 1308, 1466, 1491
MhlI GDGCHC 2 cut(s) 750, 1438
MlsI TGGCCA 1 cut(s) 1073
MluCI AATT 4 cut(s) 664, 798, 869, 1469
MluNI TGGCCA 1 cut(s) 1073
Mly113I GGCGCC 1 cut(s) 213
MlyI GAGTC 3 cut(s) 667, 956, 995
MmeI TCCRAC 2 cut(s) 1068, 1218
MnlI CCTC 7 cut(s) 557, 616, 726, 967, 1363, 1430, 1470
Mox20I TGGCCA 1 cut(s) 1073
Mph1103I ATGCAT 1 cut(s) 990
MroI TCCGGA 1 cut(s) 1171
MscI TGGCCA 1 cut(s) 1073
MseI TTAA 6 cut(s) 53, 531, 801, 947, 1055, 1472
MslI CAYNNNNRTG 3 cut(s) 810, 860, 1029
Msp20I TGGCCA 1 cut(s) 1073
MspA1I CMGCKG 2 cut(s) 249, 641
MspI CCGG 4 cut(s) 1083, 1109, 1116, 1172
MspR9I CCNGG 5 cut(s) 180, 374, 675, 1109, 1116
Mva1269I GAATGC 1 cut(s) 988
MvaI CCWGG 3 cut(s) 180, 374, 675
MwoI GCNNNNNNNGC 4 cut(s) 246, 374, 468, 1406
NarI GGCGCC 1 cut(s) 213
NciI CCSGG 2 cut(s) 1109, 1116
NdeII GATC 9 cut(s) 268, 273, 338, 352, 489, 780, 1017, 1168, 1344
NlaIII CATG 7 cut(s) 389, 599, 733, 859, 911, 941, 1028
NlaIV GGNNCC 5 cut(s) 75, 214, 886, 1107, 1113
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 784
NsbI TGCGCA 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 990
NspI RCATGY 1 cut(s) 941
PagI TCATGA 1 cut(s) 385
PceI AGGCCT 1 cut(s) 1444
PctI GAATGC 1 cut(s) 988
PfeI GAWTC 5 cut(s) 4, 334, 1124, 1227, 1293
PfoI TCCNGGA 2 cut(s) 178, 673
PkrI GCNGC 9 cut(s) 248, 251, 582, 640, 643, 833, 836, 1149, 1490
PleI GAGTC 3 cut(s) 666, 956, 995
PluTI GGCGCC 1 cut(s) 216
PpsI GAGTC 3 cut(s) 666, 956, 995
PpuMI RGGWCCY 1 cut(s) 885
PshAI GACNNNNGTC 1 cut(s) 706
PshBI ATTAAT 1 cut(s) 531
PsiI TTATAA 1 cut(s) 651
Psp5II RGGWCCY 1 cut(s) 885
Psp6I CCWGG 3 cut(s) 178, 372, 673
PspGI CCWGG 3 cut(s) 178, 372, 673
PspN4I GGNNCC 5 cut(s) 75, 214, 886, 1107, 1113
PspPI GGNCC 5 cut(s) 182, 754, 885, 1105, 1111
PspPPI RGGWCCY 1 cut(s) 885
PstI CTGCAG 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 965
RsaI GTAC 1 cut(s) 129
RsaNI GTAC 1 cut(s) 128
RseI CAYNNNNRTG 3 cut(s) 810, 860, 1029
SaqAI TTAA 6 cut(s) 53, 531, 801, 947, 1055, 1472
SatI GCNGC 9 cut(s) 247, 250, 581, 639, 642, 832, 835, 1148, 1489
Sau3AI GATC 9 cut(s) 268, 273, 338, 352, 489, 780, 1017, 1168, 1344
Sau96I GGNCC 5 cut(s) 182, 754, 885, 1105, 1111
SchI GAGTC 3 cut(s) 667, 956, 995
ScrFI CCNGG 5 cut(s) 180, 374, 675, 1109, 1116
SduI GDGCHC 2 cut(s) 750, 1438
SfaNI GCATC 2 cut(s) 249, 1248
SfcI CTRYAG 3 cut(s) 163, 244, 1320
SfoI GGCGCC 1 cut(s) 214
SinI GGWCC 4 cut(s) 182, 885, 1105, 1111
SmiMI CAYNNNNRTG 3 cut(s) 810, 860, 1029
SmlI CTYRAG 1 cut(s) 1421
SmoI CTYRAG 1 cut(s) 1421
Sse9I AATT 4 cut(s) 664, 798, 869, 1469
SseBI AGGCCT 1 cut(s) 1444
SsiI CCGC 6 cut(s) 77, 83, 122, 249, 639, 1400
SspDI GGCGCC 1 cut(s) 212
SspI AATATT 1 cut(s) 347
SspMI CTAG 2 cut(s) 452, 629
StuI AGGCCT 1 cut(s) 1444
StyD4I CCNGG 5 cut(s) 178, 372, 673, 1107, 1114
StyI CCWWGG 1 cut(s) 932
TaaI ACNGT 3 cut(s) 152, 716, 742
TaqI TCGA 4 cut(s) 207, 337, 762, 894
TasI AATT 4 cut(s) 664, 798, 869, 1469
TauI GCSGC 2 cut(s) 252, 641
TfiI GAWTC 5 cut(s) 4, 334, 1124, 1227, 1293
Tru1I TTAA 6 cut(s) 53, 531, 801, 947, 1055, 1472
Tru9I TTAA 6 cut(s) 53, 531, 801, 947, 1055, 1472
TscAI CASTG 3 cut(s) 137, 499, 514
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 7 cut(s) 246, 580, 641, 831, 834, 1147, 1488
Tsp45I GTSAC 1 cut(s) 784
TspDTI ATGAA 6 cut(s) 517, 882, 893, 1013, 1137, 1148
TspGWI ACGGA 1 cut(s) 535
TspRI CASTG 3 cut(s) 137, 499, 514
VneI GTGCAC 1 cut(s) 746
VpaK11BI GGWCC 4 cut(s) 182, 885, 1105, 1111
VspI ATTAAT 1 cut(s) 531
XapI RAATTY 1 cut(s) 869
XceI RCATGY 1 cut(s) 941
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmiI GTMKAC 3 cut(s) 406, 726, 999
XspI CTAG 2 cut(s) 452, 629
Zsp2I ATGCAT 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.