Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0001114

Physical Location & Seq

Genomic Coordinates Reverse (-)

48203978 .. 48223430

Gene Structure 19453 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG352100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGGCTTGTAAGATCCCAGCTGCACATATGTCATCTGCTAGTCCAAATGCTCTTCTATCCATAGGTGTGAAGGCACCAGCAGTAGATGCTCGTCTCCAAAGTCCCTCTGACAGTAGTCCAGGCCTCCCTTCTAGATTTGAATACCTATTGTCCGCAGAAAAGGCTAAATCCCGAAGTCTTGCGAATGCATTATCAGCCAACTCCAGCCTCAGAATTCCCTTCCCATTTGACGTAAAAAGAGCAAAAGAATTTTATCTGAGCGACGGGGTTGACTTTAGGCAAAGCAATAACAATGCTACTAATATTAACTTCTGTTTTGAGAAAGCTTTGAGTTTAGCCGGTTTTGGGTATTGGTGCAGCATCCAAGGGGATGCTTATGGCAATGAATATGGCTATGGCTTTGGGTTATATGCATATGCCTATAACTATGAAACAAAGACACTTCATCCCACTGATGTATGCCAAGCTATCGAGGAACAGGAGTGGAAGGAAGTACCCGAGTACTGTTACTATGGTGTTTGTCCACTTCCTAGGACCTTCGATGCTTGTTCATATCGGTCTGGAATTATACGATCTATACAGAAACGATCAGGAAATCATGGTTCGATGTTTTCTTTGGCTTCCCCTATGTATTTGCCAGTCCCTTTCATTTTAGGTTGTTACTTCTTTCTTTCTCTGCTTTTGTACGTGCTCTTGGGTATTTATTCTAGTGTGAAGAAAAACATCTTCATTCATCTTAAATTCAAGTTGTTCAAAAAAAATGGAGGGTTATTGTTAGAGCAGCATATTGCTAATTCTCATGGACCTGGAGCAGGATGCACAAAAATTTTCACAGCCGAGGAGCTTGTGATGGAGATCATTTTTGCACAAGAAAAGGTACTAGTGGAGGAGGGCCAAATCGAGTGTTTTATAAATGAGATTATCACTCTTGCCCATATCAACCATCAAAATGTGATCAAGTTCTTGGGCTGCTGCTTAGAGACTGAAGCTCCCATTTTGGTTTATGAATTCACCTCTAACGGGACCCTTTTCGATTACATTCATGATTGGAAAAATGGGAAAGTTTCAATGCCACATTGGGATATCCTTATGGAGATAGCATCAGAATCTGCAGCAGGTCTTGCTTATTTGCATTCTTCATCAATCATTCATGGAAATGTCAAATCTTCCAACATTTTACTAACTGATTGCTTGGCCAAAGTTGCTGGTTTTGGACCTCCAAGTTTAGTCCCTAGCAATGAGTCCCAGATAAGTACATTGGTGCAGAGGACACTCGGTTATCTGGACCCAGAGTATTTCGTTACAGGCCAATTGACAGATAAGAGTGATGTCTACAGCTTTGGAGTCGTTTTGGTAGAAATTTTAACTGGAGAGAAGCCCATATCCTTTAATAGACCAGAAAATCAACGGATCATAACTTGTCTTTTCCTTTTGTCCATGGATCAACAAAATAGCCTGTTTCAGATTCTTGCTCCACAAGTTAAGGAAGAAAACAAAGAACAAGTCAGAGCAGTCGCAGAACTCGCAAAGCAATGCCTCAAGTTGAGTAGTGCAGAAAGACCAACAATGGAAGAAGTGGCCGGAGAGTTAAAGAGGCTGTCACTCCTGTCAGACACGTATCTTACGGTTCATGTTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005975 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006884 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0008361 GO:0009311 GO:0009505 GO:0009605 GO:0009607 GO:0009615 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009826 GO:0009987 GO:0009992 GO:0010033 GO:0014070 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019725 GO:0023052 GO:0030104 GO:0030312 GO:0032502 GO:0032535 GO:0032989 GO:0036211 GO:0040007 GO:0042221 GO:0042493 GO:0042592 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055082 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0090066 GO:0098542 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

546

Amino Acids

60.64

Weight (kDa)

5.86

Isoelectric Point (pI)

50.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	294 - 526	9.1e-33	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	297 - 529	2e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001114)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g22042 FvH4_1g22043 FvH4_4g18290 FvH4_5g14090 FvH4_5g14180 FvH4_7g33870
malus_domestica	MD01G1234700.v1.1 MD05G1077200.v1.1 MD07G1307300.v1.1 MD07G1307900.v1.1
prunus_persica	Prupe.2G325000_v2.0.a1
pyrus_communis	pycom01g24290
rosa_chinensis	RchiOBHm_Chr1g0383981 RchiOBHm_Chr2g0116101 RchiOBHm_Chr2g0116111 RchiOBHm_Chr2g0130571 RchiOBHm_Chr2g0130581 RchiOBHm_Chr5g0047041
rosa_laevigata	RLG00000018239 RLG00000018240 RLG00000019138 RLG00000019139 RLG00000026080 RLG00000026081
rosa_multiflora	Rmu_co8462601.1_g000001 Rmu_sc0001968.1_g000001 Rmu_sc0002288.1_g000011 Rmu_sc0005139.1_g000022 Rmu_sc0006388.1_g000031 Rmu_sc0006388.1_g000032 Rmu_sc0038649.1_g000001
rosa_roxburghii	Rroxscaffold_1G00034400 Rroxscaffold_2G00113900 Rroxscaffold_2G00113940 Rroxscaffold_4G00320840 Rroxscaffold_5G00364610
rosa_rugosa	Rorug01G0183500 Rorug01G0444000 Rorug02G0292100 Rorug02G0292200 Rorug02G0292300 Rorug02G0292500 Rorug02G0292500 Rorug02G0292500 Rorug02G0385800 Rorug05G0233500
rosa_samantha	Rh1AG469600 Rh2AG259900 Rh2AG343600 Rh2AG343700 Rh2BG352100 Rh2CG266200 Rh2CG330900 Rh2DG268100 Rh2DG370300 Rh5AG312600 Rh5DG332700
rosa_wichuraiana	Rw2G020350 Rw5G029220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	911
Acc36I	ACCTGC	1 cut(s)	1106
AccB1I	GGYRCC	1 cut(s)	73
AccI	GTMKAC	1 cut(s)	1332
AciI	CCGC	1 cut(s)	153
AclWI	GGATC	3 cut(s)	7, 1418, 1449
AcoI	YGGCCR	2 cut(s)	1194, 1578
AcsI	RAATTY	6 cut(s)	213, 248, 740, 825, 1007, 1359
AcuI	CTGAAG	1 cut(s)	1005
AfaI	GTAC	5 cut(s)	495, 503, 686, 879, 1255
AfiI	CCNNNNNNNGG	3 cut(s)	345, 812, 1020
AflIII	ACRYGT	1 cut(s)	1614
AgsI	TTSAA	4 cut(s)	140, 745, 754, 1068
AhlI	ACTAGT	1 cut(s)	880
AjnI	CCWGG	2 cut(s)	118, 805
AluBI	AGCT	6 cut(s)	20, 326, 467, 844, 989, 1338
AluI	AGCT	6 cut(s)	20, 326, 467, 844, 989, 1338
Alw21I	GWGCWC	1 cut(s)	693
Alw26I	GTCTC	2 cut(s)	98, 974
AlwI	GGATC	3 cut(s)	7, 1418, 1449
AlwNI	CAGNNNCTG	1 cut(s)	1109
Ama87I	CYCGRG	1 cut(s)	497
AoxI	GGCC	5 cut(s)	121, 892, 1194, 1306, 1578
ApeKI	GCWGC	6 cut(s)	20, 357, 781, 969, 972, 1112
ApoI	RAATTY	6 cut(s)	213, 248, 740, 825, 1007, 1359
ArsI	GACNNNNNNTTYG	2 cut(s)	1488, 1520
AspA2I	CCTAGG	1 cut(s)	530
AspS9I	GGNCC	6 cut(s)	534, 803, 892, 1023, 1214, 1285
AsuHPI	GGTGA	1 cut(s)	1003
AvaI	CYCGRG	1 cut(s)	497
AvaII	GGWCC	5 cut(s)	534, 803, 1023, 1214, 1285
AvrII	CCTAGG	1 cut(s)	530
BalI	TGGCCA	1 cut(s)	1196
BanI	GGYRCC	1 cut(s)	73
Bbv12I	GWGCWC	1 cut(s)	693
BbvI	GCAGC	6 cut(s)	7, 369, 793, 956, 959, 1124
BccI	CCATC	2 cut(s)	844, 951
BciT130I	CCWGG	2 cut(s)	120, 807
BclI	TGATCA	1 cut(s)	954
BcoDI	GTCTC	2 cut(s)	98, 974
BcuI	ACTAGT	1 cut(s)	880
BfaI	CTAG	6 cut(s)	39, 132, 531, 708, 881, 1233
BfmI	CTRYAG	2 cut(s)	1110, 1333
BfuAI	ACCTGC	1 cut(s)	1106
BisI	GCNGC	6 cut(s)	21, 358, 782, 970, 973, 1113
BlnI	CCTAGG	1 cut(s)	530
BlsI	GCNGC	6 cut(s)	22, 359, 783, 971, 974, 1114
BmcAI	AGTACT	1 cut(s)	503
Bme1390I	CCNGG	2 cut(s)	120, 807
Bme18I	GGWCC	5 cut(s)	534, 803, 1023, 1214, 1285
BmeT110I	CYCGRG	1 cut(s)	497
BmgT120I	GGNCC	6 cut(s)	534, 803, 892, 1023, 1214, 1285
BmiI	GGNNCC	4 cut(s)	75, 1024, 1025, 1287
BmrFI	CCNGG	2 cut(s)	120, 807
BmsI	GCATC	6 cut(s)	76, 361, 369, 532, 806, 1109
BoxI	GACNNNNGTC	1 cut(s)	114
BpmI	CTGGAG	3 cut(s)	187, 828, 1389
BpuEI	CTTGAG	1 cut(s)	1523
BsaAI	YACGTR	2 cut(s)	688, 1617
BsaBI	GATNNNNATC	1 cut(s)	854
BsaJI	CCNNGG	4 cut(s)	364, 530, 837, 1437
BsaXI	ACNNNNNCTCC	2 cut(s)	973, 1003
Bsc4I	CCNNNNNNNGG	3 cut(s)	345, 812, 1020
Bse118I	RCCGGY	1 cut(s)	338
Bse1I	ACTGG	2 cut(s)	638, 1372
Bse3DI	GCAATG	3 cut(s)	388, 1243, 1538
Bse8I	GATNNNNATC	1 cut(s)	854
BseBI	CCWGG	2 cut(s)	120, 807
BseDI	CCNNGG	4 cut(s)	364, 530, 837, 1437
BseGI	GGATG	4 cut(s)	360, 376, 445, 821
BseJI	GATNNNNATC	1 cut(s)	854
BseLI	CCNNNNNNNGG	3 cut(s)	345, 812, 1020
BseMI	GCAATG	3 cut(s)	388, 1243, 1538
BseMII	CTCAG	2 cut(s)	223, 248
BseNI	ACTGG	2 cut(s)	638, 1372
BseRI	GAGGAG	2 cut(s)	854, 902
BseXI	GCAGC	6 cut(s)	7, 369, 793, 956, 959, 1124
BseYI	CCCAGC	1 cut(s)	16
BsgI	GTGCAG	4 cut(s)	6, 376, 1283, 1572
BshFI	GGCC	5 cut(s)	123, 894, 1196, 1308, 1580
BshNI	GGYRCC	1 cut(s)	73
BsiHKAI	GWGCWC	1 cut(s)	693
BsiHKCI	CYCGRG	1 cut(s)	497
BsiSI	CCGG	2 cut(s)	339, 1581
BslFI	GGGAC	5 cut(s)	87, 626, 1036, 1214, 1228
BslI	CCNNNNNNNGG	3 cut(s)	345, 812, 1020
BsmAI	GTCTC	2 cut(s)	98, 974
BsmBI	CGTCTC	1 cut(s)	98
BsmFI	GGGAC	5 cut(s)	87, 626, 1036, 1214, 1228
BsmI	GAATGC	2 cut(s)	190, 1132
BsnI	GGCC	5 cut(s)	123, 894, 1196, 1308, 1580
BsoBI	CYCGRG	1 cut(s)	497
Bsp1286I	GDGCHC	1 cut(s)	693
Bsp143I	GATC	7 cut(s)	12, 572, 587, 855, 954, 1410, 1441
Bsp19I	CCATGG	1 cut(s)	1437
BspACI	CCGC	1 cut(s)	153
BspANI	GGCC	5 cut(s)	123, 894, 1196, 1308, 1580
BspCNI	CTCAG	2 cut(s)	222, 249
BspHI	TCATGA	1 cut(s)	1042
BspLI	GGNNCC	4 cut(s)	75, 1024, 1025, 1287
BspMAI	CTGCAG	1 cut(s)	1114
BspMI	ACCTGC	1 cut(s)	1106
BspPI	GGATC	3 cut(s)	7, 1418, 1449
BspQI	GCTCTTC	1 cut(s)	57
BspT107I	GGYRCC	1 cut(s)	73
BsrDI	GCAATG	3 cut(s)	388, 1243, 1538
BsrFI	RCCGGY	1 cut(s)	338
BsrI	ACTGG	2 cut(s)	638, 1372
BssAI	RCCGGY	1 cut(s)	338
BssECI	CCNNGG	4 cut(s)	364, 530, 837, 1437
BssMI	GATC	7 cut(s)	12, 572, 587, 855, 954, 1410, 1441
BssT1I	CCWWGG	3 cut(s)	364, 530, 1437
Bst2UI	CCWGG	2 cut(s)	120, 807
Bst4CI	ACNGT	3 cut(s)	113, 506, 1627
Bst6I	CTCTTC	1 cut(s)	57
BstAPI	GCANNNNNTGC	2 cut(s)	86, 1121
BstBAI	YACGTR	2 cut(s)	688, 1617
BstDEI	CTNAG	3 cut(s)	209, 257, 976
BstDSI	CCRYGG	1 cut(s)	1437
BstENI	CCTNNNNNAGG	1 cut(s)	810
BstF5I	GGATG	4 cut(s)	360, 376, 445, 821
BstKTI	GATC	7 cut(s)	15, 575, 590, 858, 957, 1413, 1444
BstMAI	GTCTC	2 cut(s)	98, 974
BstMBI	GATC	7 cut(s)	12, 572, 587, 855, 954, 1410, 1441
BstMWI	GCNNNNNNNGC	6 cut(s)	86, 161, 194, 1121, 1202, 1523
BstNI	CCWGG	2 cut(s)	120, 807
BstPAI	GACNNNNGTC	1 cut(s)	114
BstSCI	CCNGG	2 cut(s)	118, 805
BstSFI	CTRYAG	2 cut(s)	1110, 1333
BstV1I	GCAGC	6 cut(s)	7, 369, 793, 956, 959, 1124
BstX2I	RGATCY	1 cut(s)	12
BstYI	RGATCY	1 cut(s)	12
BsuRI	GGCC	5 cut(s)	123, 894, 1196, 1308, 1580
BtgI	CCRYGG	1 cut(s)	1437
BtsCI	GGATG	4 cut(s)	360, 376, 445, 821
BtsIMutI	CAGTG	1 cut(s)	450
BveI	ACCTGC	1 cut(s)	1106
CaiI	CAGNNNCTG	1 cut(s)	1109
CciI	TCATGA	1 cut(s)	1042
Cfr10I	RCCGGY	1 cut(s)	338
Cfr13I	GGNCC	6 cut(s)	534, 803, 892, 1023, 1214, 1285
Csp6I	GTAC	5 cut(s)	494, 502, 685, 878, 1254
CviAII	CATG	6 cut(s)	599, 800, 1043, 1151, 1438, 1631
CviQI	GTAC	5 cut(s)	494, 502, 685, 878, 1254
DdeI	CTNAG	3 cut(s)	209, 257, 976
DpnI	GATC	7 cut(s)	14, 574, 589, 857, 956, 1412, 1443
DpnII	GATC	7 cut(s)	12, 572, 587, 855, 954, 1410, 1441
EaeI	YGGCCR	2 cut(s)	1194, 1578
Eam1104I	CTCTTC	1 cut(s)	57
EarI	CTCTTC	1 cut(s)	57
Eco130I	CCWWGG	3 cut(s)	364, 530, 1437
Eco147I	AGGCCT	1 cut(s)	123
Eco32I	GATATC	1 cut(s)	1084
Eco47I	GGWCC	5 cut(s)	534, 803, 1023, 1214, 1285
Eco57I	CTGAAG	1 cut(s)	1005
Eco88I	CYCGRG	1 cut(s)	497
EcoNI	CCTNNNNNAGG	1 cut(s)	810
EcoO109I	RGGNCCY	2 cut(s)	534, 1023
EcoRI	GAATTC	2 cut(s)	213, 1007
EcoRII	CCWGG	2 cut(s)	118, 805
EcoRV	GATATC	1 cut(s)	1084
EcoT14I	CCWWGG	3 cut(s)	364, 530, 1437
EcoT22I	ATGCAT	2 cut(s)	190, 415
ErhI	CCWWGG	3 cut(s)	364, 530, 1437
Esp3I	CGTCTC	1 cut(s)	98
FaeI	CATG	6 cut(s)	602, 803, 1046, 1154, 1441, 1634
FaqI	GGGAC	5 cut(s)	87, 626, 1036, 1214, 1228
FatI	CATG	6 cut(s)	598, 799, 1042, 1150, 1437, 1630
FauNDI	CATATG	2 cut(s)	27, 415
FbaI	TGATCA	1 cut(s)	954
FblI	GTMKAC	1 cut(s)	1332
Fnu4HI	GCNGC	6 cut(s)	21, 358, 782, 970, 973, 1113
FokI	GGATG	4 cut(s)	347, 383, 432, 828
Fsp4HI	GCNGC	6 cut(s)	21, 358, 782, 970, 973, 1113
FspBI	CTAG	6 cut(s)	39, 132, 531, 708, 881, 1233
GluI	GCNGC	6 cut(s)	21, 358, 782, 970, 973, 1113
GsaI	CCCAGC	1 cut(s)	20
GsuI	CTGGAG	3 cut(s)	187, 828, 1389
HaeIII	GGCC	5 cut(s)	123, 894, 1196, 1308, 1580
HapII	CCGG	2 cut(s)	339, 1581
Hin1II	CATG	6 cut(s)	602, 803, 1046, 1154, 1441, 1634
HincII	GTYRAC	1 cut(s)	271
HindII	GTYRAC	1 cut(s)	271
HindIII	AAGCTT	1 cut(s)	324
HinfI	GANTC	4 cut(s)	1106, 1241, 1344, 1465
HpaII	CCGG	2 cut(s)	339, 1581
HphI	GGTGA	1 cut(s)	1003
Hpy166II	GTNNAC	3 cut(s)	271, 524, 1333
Hpy188I	TCNGA	7 cut(s)	109, 212, 258, 1105, 1464, 1508, 1612
Hpy188III	TCNNGA	6 cut(s)	132, 171, 561, 591, 1043, 1283
Hpy8I	GTNNAC	3 cut(s)	271, 524, 1333
Hpy99I	CGWCG	1 cut(s)	266
HpyAV	CCTTC	5 cut(s)	64, 138, 229, 481, 547
HpyCH4III	ACNGT	3 cut(s)	113, 506, 1627
HpyCH4IV	ACGT	3 cut(s)	231, 687, 1616
HpyF10VI	GCNNNNNNNGC	6 cut(s)	86, 161, 194, 1121, 1202, 1523
HpyF3I	CTNAG	3 cut(s)	209, 257, 976
HpySE526I	ACGT	3 cut(s)	231, 687, 1616
Hsp92II	CATG	6 cut(s)	602, 803, 1046, 1154, 1441, 1634
KflI	GGGWCCC	1 cut(s)	1023
Ksp22I	TGATCA	1 cut(s)	954
Kzo9I	GATC	7 cut(s)	12, 572, 587, 855, 954, 1410, 1441
LguI	GCTCTTC	1 cut(s)	57
LmnI	GCTCC	4 cut(s)	809, 841, 994, 1477
Lsp1109I	GCAGC	6 cut(s)	7, 369, 793, 956, 959, 1124
LweI	GCATC	6 cut(s)	76, 361, 369, 532, 806, 1109
MaeI	CTAG	6 cut(s)	39, 132, 531, 708, 881, 1233
MaeII	ACGT	3 cut(s)	231, 687, 1616
MaeIII	GTNAC	4 cut(s)	506, 659, 1300, 1599
MalI	GATC	7 cut(s)	14, 574, 589, 857, 956, 1412, 1443
MboI	GATC	7 cut(s)	12, 572, 587, 855, 954, 1410, 1441
MboII	GAAGA	7 cut(s)	44, 718, 727, 1128, 1158, 1499, 1583
MfeI	CAATTG	1 cut(s)	1310
MflI	RGATCY	1 cut(s)	12
MhlI	GDGCHC	1 cut(s)	693
MlsI	TGGCCA	1 cut(s)	1196
MluCI	AATT	9 cut(s)	213, 248, 564, 740, 793, 825, 1007, 1310, 1359
MluNI	TGGCCA	1 cut(s)	1196
MlyI	GAGTC	2 cut(s)	1250, 1353
MmeI	TCCRAC	1 cut(s)	1194
Mox20I	TGGCCA	1 cut(s)	1196
Mph1103I	ATGCAT	2 cut(s)	190, 415
MscI	TGGCCA	1 cut(s)	1196
MseI	TTAA	7 cut(s)	306, 738, 1364, 1389, 1482, 1589, 1639
MslI	CAYNNNNRTG	3 cut(s)	65, 948, 1155
Msp20I	TGGCCA	1 cut(s)	1196
MspA1I	CMGCKG	1 cut(s)	20
MspI	CCGG	2 cut(s)	339, 1581
MspR9I	CCNGG	2 cut(s)	120, 807
MunI	CAATTG	1 cut(s)	1310
Mva1269I	GAATGC	2 cut(s)	190, 1132
MvaI	CCWGG	2 cut(s)	120, 807
MwoI	GCNNNNNNNGC	6 cut(s)	86, 161, 194, 1121, 1202, 1523
NcoI	CCATGG	1 cut(s)	1437
NdeI	CATATG	2 cut(s)	27, 415
NdeII	GATC	7 cut(s)	12, 572, 587, 855, 954, 1410, 1441
NlaIII	CATG	6 cut(s)	602, 803, 1046, 1154, 1441, 1634
NlaIV	GGNNCC	4 cut(s)	75, 1024, 1025, 1287
NmeAIII	GCCGAG	1 cut(s)	862
NmuCI	GTSAC	1 cut(s)	1599
NsiI	ATGCAT	2 cut(s)	190, 415
PagI	TCATGA	1 cut(s)	1042
PceI	AGGCCT	1 cut(s)	123
PciSI	GCTCTTC	1 cut(s)	57
PctI	GAATGC	2 cut(s)	190, 1132
PfeI	GAWTC	2 cut(s)	1106, 1465
PkrI	GCNGC	6 cut(s)	22, 359, 783, 971, 974, 1114
PleI	GAGTC	2 cut(s)	1249, 1352
PpsI	GAGTC	2 cut(s)	1249, 1352
Ppu21I	YACGTR	2 cut(s)	688, 1617
PpuMI	RGGWCCY	2 cut(s)	534, 1023
PshAI	GACNNNNGTC	1 cut(s)	114
PsiI	TTATAA	1 cut(s)	911
Psp5II	RGGWCCY	2 cut(s)	534, 1023
Psp6I	CCWGG	2 cut(s)	118, 805
PspFI	CCCAGC	1 cut(s)	16
PspGI	CCWGG	2 cut(s)	118, 805
PspN4I	GGNNCC	4 cut(s)	75, 1024, 1025, 1287
PspPI	GGNCC	6 cut(s)	534, 803, 892, 1023, 1214, 1285
PspPPI	RGGWCCY	2 cut(s)	534, 1023
PstI	CTGCAG	1 cut(s)	1114
PstNI	CAGNNNCTG	1 cut(s)	1109
PsuI	RGATCY	1 cut(s)	12
PvuII	CAGCTG	1 cut(s)	20
RsaI	GTAC	5 cut(s)	495, 503, 686, 879, 1255
RsaNI	GTAC	5 cut(s)	494, 502, 685, 878, 1254
RseI	CAYNNNNRTG	3 cut(s)	65, 948, 1155
SapI	GCTCTTC	1 cut(s)	57
SaqAI	TTAA	7 cut(s)	306, 738, 1364, 1389, 1482, 1589, 1639
SatI	GCNGC	6 cut(s)	21, 358, 782, 970, 973, 1113
Sau3AI	GATC	7 cut(s)	12, 572, 587, 855, 954, 1410, 1441
Sau96I	GGNCC	6 cut(s)	534, 803, 892, 1023, 1214, 1285
ScaI	AGTACT	1 cut(s)	503
SchI	GAGTC	2 cut(s)	1250, 1353
ScrFI	CCNGG	2 cut(s)	120, 807
SduI	GDGCHC	1 cut(s)	693
SfaNI	GCATC	6 cut(s)	76, 361, 369, 532, 806, 1109
SfcI	CTRYAG	2 cut(s)	1110, 1333
SinI	GGWCC	5 cut(s)	534, 803, 1023, 1214, 1285
SmiMI	CAYNNNNRTG	3 cut(s)	65, 948, 1155
SmlI	CTYRAG	1 cut(s)	1538
SmoI	CTYRAG	1 cut(s)	1538
SpeI	ACTAGT	1 cut(s)	880
Sse9I	AATT	9 cut(s)	213, 248, 564, 740, 793, 825, 1007, 1310, 1359
SseBI	AGGCCT	1 cut(s)	123
SsiI	CCGC	1 cut(s)	153
SspI	AATATT	1 cut(s)	304
SspMI	CTAG	6 cut(s)	39, 132, 531, 708, 881, 1233
StuI	AGGCCT	1 cut(s)	123
StyD4I	CCNGG	2 cut(s)	118, 805
StyI	CCWWGG	3 cut(s)	364, 530, 1437
TaaI	ACNGT	3 cut(s)	113, 506, 1627
TaiI	ACGT	3 cut(s)	234, 690, 1619
TaqI	TCGA	5 cut(s)	471, 540, 605, 900, 1032
TaqII	GACCGA	1 cut(s)	546
TasI	AATT	9 cut(s)	213, 248, 564, 740, 793, 825, 1007, 1310, 1359
TatI	WGTACW	2 cut(s)	501, 1253
TfiI	GAWTC	2 cut(s)	1106, 1465
Tru1I	TTAA	7 cut(s)	306, 738, 1364, 1389, 1482, 1589, 1639
Tru9I	TTAA	7 cut(s)	306, 738, 1364, 1389, 1482, 1589, 1639
TscAI	CASTG	1 cut(s)	457
TseFI	GTSAC	1 cut(s)	1599
TseI	GCWGC	6 cut(s)	20, 357, 781, 969, 972, 1112
Tsp45I	GTSAC	1 cut(s)	1599
TspGWI	ACGGA	1 cut(s)	1423
TspRI	CASTG	1 cut(s)	457
VpaK11BI	GGWCC	5 cut(s)	534, 803, 1023, 1214, 1285
XagI	CCTNNNNNAGG	1 cut(s)	810
XapI	RAATTY	6 cut(s)	213, 248, 740, 825, 1007, 1359
XbaI	TCTAGA	1 cut(s)	131
XmaJI	CCTAGG	1 cut(s)	530
XmiI	GTMKAC	1 cut(s)	1332
XspI	CTAG	6 cut(s)	39, 132, 531, 708, 881, 1233
ZrmI	AGTACT	1 cut(s)	503
Zsp2I	ATGCAT	2 cut(s)	190, 415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.