Rorug02G0292300

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001114
Physical Location & Seq
Forward (+)
32263861 .. 32265425
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0292300.1

Sequence Viewer

Length: 1101 bp
ATGGAGGATGAGCTCAAGAACTTCATCAAGGTATGGATCACAACCATCATATGTCTATCTTATTGCCATTACATAGTCTCCAGATTAATCCCCAAGGGCCTGCTTAGGCTCATCTCTCTCCTCCCTGTCTTCTACTTCTTCATCACCACCCCCCTAACTCTCCATTCCTTCCATCTCTGTGGACCCACAACCTTCTTCCTAGTCTGGCTTGGCATCTTCAAGCTCCTCCTCTTCTCCTTTAACCTTGGCCCTCTATCACCACCCCCACCTTCACTATTCCATTTCATCTCCATAGCTTGCCTCCCCATCAAAATCAAACAAGACCCACTTCGGAAAACACCCCCAAATCCCAAAACCACCCAAAAACCCAGAAACCCAATTGGTCAAAGTGTCACAAAGGGGCTGCCAAACAAGTCAATTGTGGTGGCTGTGAAGGCATTGCTTTTGGGTATGATCATCCATACCTACGAGTACAGATCACATTTGCACCCTTATGTGATCTTAGCCTTGTACTGTTGCCACATGTACCTGGCTCTAGAATTAGTCCTGGTCGTGAGCGCGACTCCGGCTCGAGCTCTTTTCGGATTTGAGCTCGAGCCGCAGTCCAACGAGCCTTACCTCTCCACTTCGCTTCAAGACTTTTGGGGCCGTCGATGGAATCTAATGGTCACGAACATCCTGCGCCCAACGGTATACGATCCCACCCGGCGCGTGTGTGCGCGTGTAGTTGGGAAACGGTGGGCAAGGCTGCCGGCTGTGATGGCCACCTTTGCGGTGTCCGGGTTAATGCACGAGGTTATTTATTATTATCTCACCCGCGTGCAGCCCACGTGGGAAGTGACGTGGTTTTTTGTGCTACACGGGGCTTGCGTGGTGGTTGAGGTGGAGGTCAAGAAGGCGGTTACCGGCAGGTGGAGGTTGCACCCGGTGGTTTCGGGGCCGTTGACGTTGGTGTTCTTGGCTGTGACCGGCAACTGGCTGTTCTTTCCTCAGTTGCTCAGGAATGGTGTTGATGCAAAGGCTATAGGAGAGTATGCAATTATGGTGGATTTTGTCAAGGCTAACTTGCCTCATTTAAGTTATTGGCCCAACAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

366

Amino Acids

41.79

Weight (kDa)

9.66

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 199 - 285 7.4e-20 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 900
Acc36I ACCTGC 1 cut(s) 900
AccI GTMKAC 1 cut(s) 693
AccII CGCG 4 cut(s) 560, 711, 721, 819
AciI CCGC 4 cut(s) 599, 773, 817, 899
AclWI GGATC 2 cut(s) 44, 692
AcoI YGGCCR 1 cut(s) 762
AcvI CACGTG 1 cut(s) 831
AdeI CACNNNGTG 1 cut(s) 928
AfaI GTAC 3 cut(s) 473, 512, 527
AfiI CCNNNNNNNGG 2 cut(s) 912, 975
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 2 cut(s) 220, 635
AjiI CACGTC 1 cut(s) 843
AjnI CCWGG 2 cut(s) 528, 546
AleI CACNNNNGTG 1 cut(s) 818
AluBI AGCT 6 cut(s) 13, 223, 296, 575, 592, 1098
AluI AGCT 6 cut(s) 13, 223, 296, 575, 592, 1098
Alw21I GWGCWC 3 cut(s) 15, 577, 594
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 2 cut(s) 44, 692
Ama87I CYCGRG 2 cut(s) 570, 593
AoxI GGCC 6 cut(s) 97, 247, 646, 762, 938, 1085
ApeKI GCWGC 3 cut(s) 403, 748, 823
AseI ATTAAT 1 cut(s) 86
AspLEI GCGC 4 cut(s) 560, 684, 711, 721
AspS9I GGNCC 6 cut(s) 97, 182, 248, 646, 938, 1086
AsuC2I CCSGG 3 cut(s) 706, 781, 926
AsuHPI GGTGA 3 cut(s) 136, 249, 805
AvaI CYCGRG 2 cut(s) 570, 593
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 764
BanII GRGCYC 3 cut(s) 15, 577, 594
BauI CACGAG 1 cut(s) 791
BbrPI CACGTG 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 121
Bbv12I GWGCWC 3 cut(s) 15, 577, 594
BbvI GCAGC 3 cut(s) 390, 735, 835
BccI CCATC 5 cut(s) 53, 180, 314, 648, 754
BceAI ACGGC 2 cut(s) 633, 925
BcgI CGANNNNNNTGC 2 cut(s) 661, 695
BciT130I CCWGG 2 cut(s) 530, 548
BclI TGATCA 1 cut(s) 453
BcnI CCSGG 3 cut(s) 706, 781, 926
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 2 cut(s) 200, 536
BfmI CTRYAG 1 cut(s) 1023
BfuAI ACCTGC 1 cut(s) 900
BisI GCNGC 4 cut(s) 404, 599, 749, 824
BlsI GCNGC 4 cut(s) 405, 600, 750, 825
Bme1390I CCNGG 5 cut(s) 530, 548, 706, 781, 926
Bme18I GGWCC 1 cut(s) 182
BmeT110I CYCGRG 2 cut(s) 570, 593
BmgBI CACGTC 1 cut(s) 843
BmgT120I GGNCC 6 cut(s) 97, 182, 248, 646, 938, 1086
BmiI GGNNCC 3 cut(s) 184, 647, 939
BmrFI CCNGG 5 cut(s) 530, 548, 706, 781, 926
BmsI GCATC 2 cut(s) 222, 1003
BpiI GAAGAC 1 cut(s) 121
BplI GAGNNNNNCTC 2 cut(s) 547, 579
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 2 cut(s) 104, 998
BpuMI CCSGG 3 cut(s) 706, 781, 926
BsaAI YACGTR 1 cut(s) 831
BsaJI CCNNGG 2 cut(s) 93, 244
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 2 cut(s) 912, 975
Bse118I RCCGGY 3 cut(s) 751, 905, 968
Bse1I ACTGG 1 cut(s) 980
Bse3DI GCAATG 1 cut(s) 437
BseBI CCWGG 2 cut(s) 530, 548
BseDI CCNNGG 2 cut(s) 93, 244
BseGI GGATG 3 cut(s) 13, 456, 675
BseLI CCNNNNNNNGG 2 cut(s) 912, 975
BseMI GCAATG 1 cut(s) 437
BseMII CTCAG 2 cut(s) 1004, 1012
BseNI ACTGG 1 cut(s) 980
BseRI GAGGAG 3 cut(s) 110, 215, 218
BseXI GCAGC 3 cut(s) 390, 735, 835
BsgI GTGCAG 1 cut(s) 842
Bsh1236I CGCG 4 cut(s) 560, 711, 721, 819
BshFI GGCC 6 cut(s) 99, 249, 648, 764, 940, 1087
BsiHKAI GWGCWC 3 cut(s) 15, 577, 594
BsiHKCI CYCGRG 2 cut(s) 570, 593
BsiSI CCGG 7 cut(s) 566, 706, 752, 780, 906, 926, 969
BslI CCNNNNNNNGG 2 cut(s) 912, 975
BsmAI GTCTC 1 cut(s) 82
BsnI GGCC 6 cut(s) 99, 249, 648, 764, 940, 1087
BsoBI CYCGRG 2 cut(s) 570, 593
Bsp1286I GDGCHC 3 cut(s) 15, 577, 594
Bsp143I GATC 5 cut(s) 36, 453, 476, 498, 697
BspACI CCGC 4 cut(s) 599, 773, 817, 899
BspANI GGCC 6 cut(s) 99, 249, 648, 764, 940, 1087
BspCNI CTCAG 2 cut(s) 1003, 1011
BspFNI CGCG 4 cut(s) 560, 711, 721, 819
BspLI GGNNCC 3 cut(s) 184, 647, 939
BspMI ACCTGC 1 cut(s) 900
BspPI GGATC 2 cut(s) 44, 692
BsrDI GCAATG 1 cut(s) 437
BsrFI RCCGGY 3 cut(s) 751, 905, 968
BsrI ACTGG 1 cut(s) 980
BssAI RCCGGY 3 cut(s) 751, 905, 968
BssECI CCNNGG 2 cut(s) 93, 244
BssMI GATC 5 cut(s) 36, 453, 476, 498, 697
BssNAI GTATAC 1 cut(s) 694
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 2 cut(s) 93, 244
Bst1107I GTATAC 1 cut(s) 694
Bst2BI CACGAG 1 cut(s) 791
Bst2UI CCWGG 2 cut(s) 530, 548
Bst4CI ACNGT 3 cut(s) 515, 691, 738
Bst6I CTCTTC 1 cut(s) 236
BstBAI YACGTR 1 cut(s) 831
BstC8I GCNNGC 5 cut(s) 101, 298, 753, 821, 868
BstDEI CTNAG 4 cut(s) 104, 502, 990, 998
BstEII GGTNACC 1 cut(s) 901
BstF5I GGATG 3 cut(s) 13, 456, 675
BstFNI CGCG 4 cut(s) 560, 711, 721, 819
BstHHI GCGC 4 cut(s) 560, 684, 711, 721
BstKTI GATC 5 cut(s) 39, 456, 479, 501, 700
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 5 cut(s) 36, 453, 476, 498, 697
BstMWI GCNNNNNNNGC 5 cut(s) 434, 566, 598, 761, 770
BstNI CCWGG 2 cut(s) 530, 548
BstNSI RCATGY 1 cut(s) 526
BstPI GGTNACC 1 cut(s) 901
BstSCI CCNGG 5 cut(s) 528, 546, 704, 779, 924
BstSFI CTRYAG 1 cut(s) 1023
BstUI CGCG 4 cut(s) 560, 711, 721, 819
BstV1I GCAGC 3 cut(s) 390, 735, 835
BstV2I GAAGAC 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 179
BstZ17I GTATAC 1 cut(s) 694
BsuRI GGCC 6 cut(s) 99, 249, 648, 764, 940, 1087
BtrI CACGTC 1 cut(s) 843
BtsCI GGATG 3 cut(s) 13, 456, 675
BveI ACCTGC 1 cut(s) 900
Cac8I GCNNGC 5 cut(s) 101, 298, 753, 821, 868
CfoI GCGC 4 cut(s) 560, 684, 711, 721
Cfr10I RCCGGY 3 cut(s) 751, 905, 968
Cfr13I GGNCC 6 cut(s) 97, 182, 248, 646, 938, 1086
Csp6I GTAC 3 cut(s) 472, 511, 526
CspCI CAANNNNNGTGG 4 cut(s) 405, 440, 1026, 1061
CviAII CATG 1 cut(s) 523
CviQI GTAC 3 cut(s) 472, 511, 526
DdeI CTNAG 4 cut(s) 104, 502, 990, 998
DpnI GATC 5 cut(s) 38, 455, 478, 500, 699
DpnII GATC 5 cut(s) 36, 453, 476, 498, 697
DraIII CACNNNGTG 1 cut(s) 928
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Ecl136II GAGCTC 3 cut(s) 13, 575, 592
Eco130I CCWWGG 2 cut(s) 93, 244
Eco24I GRGCYC 3 cut(s) 15, 577, 594
Eco47I GGWCC 1 cut(s) 182
Eco53kI GAGCTC 3 cut(s) 13, 575, 592
Eco72I CACGTG 1 cut(s) 831
Eco88I CYCGRG 2 cut(s) 570, 593
Eco91I GGTNACC 1 cut(s) 901
EcoICRI GAGCTC 3 cut(s) 13, 575, 592
EcoO109I RGGNCCY 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 901
EcoRII CCWGG 2 cut(s) 528, 546
EcoT14I CCWWGG 2 cut(s) 93, 244
EcoT38I GRGCYC 3 cut(s) 15, 577, 594
ErhI CCWWGG 2 cut(s) 93, 244
FaeI CATG 1 cut(s) 526
FalI AAGNNNNNCTT 4 cut(s) 312, 344, 1049, 1081
FatI CATG 1 cut(s) 522
FauI CCCGC 1 cut(s) 824
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 1 cut(s) 453
FblI GTMKAC 1 cut(s) 693
Fnu4HI GCNGC 4 cut(s) 404, 599, 749, 824
FokI GGATG 3 cut(s) 20, 443, 662
FriOI GRGCYC 3 cut(s) 15, 577, 594
Fsp4HI GCNGC 4 cut(s) 404, 599, 749, 824
FspBI CTAG 2 cut(s) 200, 536
GlaI GCGC 4 cut(s) 559, 683, 710, 720
GluI GCNGC 4 cut(s) 404, 599, 749, 824
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 6 cut(s) 99, 249, 648, 764, 940, 1087
HapII CCGG 7 cut(s) 566, 706, 752, 780, 906, 926, 969
HhaI GCGC 4 cut(s) 560, 684, 711, 721
Hin1II CATG 1 cut(s) 526
Hin6I GCGC 4 cut(s) 558, 682, 709, 719
HinP1I GCGC 4 cut(s) 558, 682, 709, 719
HincII GTYRAC 1 cut(s) 945
HindII GTYRAC 1 cut(s) 945
HinfI GANTC 2 cut(s) 562, 658
HpaII CCGG 7 cut(s) 566, 706, 752, 780, 906, 926, 969
HphI GGTGA 3 cut(s) 136, 249, 805
Hpy166II GTNNAC 3 cut(s) 182, 694, 945
Hpy188I TCNGA 2 cut(s) 333, 584
Hpy188III TCNNGA 8 cut(s) 16, 81, 536, 553, 635, 670, 892, 1000
Hpy8I GTNNAC 3 cut(s) 182, 694, 945
Hpy99I CGWCG 1 cut(s) 654
HpyAV CCTTC 5 cut(s) 178, 202, 279, 427, 889
HpyCH4III ACNGT 3 cut(s) 515, 691, 738
HpyCH4IV ACGT 3 cut(s) 830, 842, 947
HpyCH4V TGCA 6 cut(s) 487, 790, 823, 922, 1016, 1037
HpyF10VI GCNNNNNNNGC 5 cut(s) 434, 566, 598, 761, 770
HpyF3I CTNAG 4 cut(s) 104, 502, 990, 998
HpySE526I ACGT 3 cut(s) 830, 842, 947
Hsp92II CATG 1 cut(s) 526
HspAI GCGC 4 cut(s) 558, 682, 709, 719
KroI GCCGGC 1 cut(s) 751
KroNI GCCGGC 1 cut(s) 753
Ksp22I TGATCA 1 cut(s) 453
Kzo9I GATC 5 cut(s) 36, 453, 476, 498, 697
LmnI GCTCC 1 cut(s) 228
Lsp1109I GCAGC 3 cut(s) 390, 735, 835
LweI GCATC 2 cut(s) 222, 1003
MaeI CTAG 2 cut(s) 200, 536
MaeII ACGT 3 cut(s) 830, 842, 947
MaeIII GTNAC 5 cut(s) 391, 667, 838, 901, 964
MalI GATC 5 cut(s) 38, 455, 478, 500, 699
MboI GATC 5 cut(s) 36, 453, 476, 498, 697
MboII GAAGA 5 cut(s) 121, 130, 187, 208, 223
MfeI CAATTG 2 cut(s) 378, 417
MhlI GDGCHC 3 cut(s) 15, 577, 594
MlsI TGGCCA 1 cut(s) 764
MluCI AATT 4 cut(s) 378, 417, 539, 1038
MluNI TGGCCA 1 cut(s) 764
MlyI GAGTC 1 cut(s) 556
MmeI TCCRAC 1 cut(s) 630
Mox20I TGGCCA 1 cut(s) 764
MroNI GCCGGC 1 cut(s) 751
MscI TGGCCA 1 cut(s) 764
MseI TTAA 4 cut(s) 86, 240, 785, 1076
MslI CAYNNNNRTG 3 cut(s) 177, 492, 818
Msp20I TGGCCA 1 cut(s) 764
MspI CCGG 7 cut(s) 566, 706, 752, 780, 906, 926, 969
MspR9I CCNGG 5 cut(s) 530, 548, 706, 781, 926
MunI CAATTG 2 cut(s) 378, 417
MvaI CCWGG 2 cut(s) 530, 548
MvnI CGCG 4 cut(s) 560, 711, 721, 819
MwoI GCNNNNNNNGC 5 cut(s) 434, 566, 598, 761, 770
NaeI GCCGGC 1 cut(s) 753
NciI CCSGG 3 cut(s) 706, 781, 926
NdeI CATATG 1 cut(s) 50
NdeII GATC 5 cut(s) 36, 453, 476, 498, 697
NgoMIV GCCGGC 1 cut(s) 751
NlaIII CATG 1 cut(s) 526
NlaIV GGNNCC 3 cut(s) 184, 647, 939
NmuCI GTSAC 4 cut(s) 391, 667, 838, 964
NspI RCATGY 1 cut(s) 526
OliI CACNNNNGTG 1 cut(s) 818
PaeR7I CTCGAG 2 cut(s) 570, 593
PaqCI CACCTGC 1 cut(s) 900
PciI ACATGT 1 cut(s) 522
PcsI WCGNNNNNNNCGW 1 cut(s) 867
PdiI GCCGGC 1 cut(s) 753
PfeI GAWTC 1 cut(s) 658
PkrI GCNGC 4 cut(s) 405, 600, 750, 825
PleI GAGTC 1 cut(s) 556
PmaCI CACGTG 1 cut(s) 831
PmlI CACGTG 1 cut(s) 831
PpsI GAGTC 1 cut(s) 556
Ppu21I YACGTR 1 cut(s) 831
PscI ACATGT 1 cut(s) 522
PshBI ATTAAT 1 cut(s) 86
Psp124BI GAGCTC 3 cut(s) 15, 577, 594
Psp6I CCWGG 2 cut(s) 528, 546
PspCI CACGTG 1 cut(s) 831
PspEI GGTNACC 1 cut(s) 901
PspGI CCWGG 2 cut(s) 528, 546
PspN4I GGNNCC 3 cut(s) 184, 647, 939
PspPI GGNCC 6 cut(s) 97, 182, 248, 646, 938, 1086
PspXI VCTCGAGB 2 cut(s) 570, 593
RsaI GTAC 3 cut(s) 473, 512, 527
RsaNI GTAC 3 cut(s) 472, 511, 526
RseI CAYNNNNRTG 3 cut(s) 177, 492, 818
SacI GAGCTC 3 cut(s) 15, 577, 594
SaqAI TTAA 4 cut(s) 86, 240, 785, 1076
SatI GCNGC 4 cut(s) 404, 599, 749, 824
Sau3AI GATC 5 cut(s) 36, 453, 476, 498, 697
Sau96I GGNCC 6 cut(s) 97, 182, 248, 646, 938, 1086
SchI GAGTC 1 cut(s) 556
ScrFI CCNGG 5 cut(s) 530, 548, 706, 781, 926
SduI GDGCHC 3 cut(s) 15, 577, 594
SfaNI GCATC 2 cut(s) 222, 1003
SfcI CTRYAG 1 cut(s) 1023
Sfr274I CTCGAG 2 cut(s) 570, 593
SinI GGWCC 1 cut(s) 182
SlaI CTCGAG 2 cut(s) 570, 593
SmiMI CAYNNNNRTG 3 cut(s) 177, 492, 818
SmlI CTYRAG 3 cut(s) 14, 570, 593
SmoI CTYRAG 3 cut(s) 14, 570, 593
Sse9I AATT 4 cut(s) 378, 417, 539, 1038
SsiI CCGC 4 cut(s) 599, 773, 817, 899
SspMI CTAG 2 cut(s) 200, 536
SstI GAGCTC 3 cut(s) 15, 577, 594
StyD4I CCNGG 5 cut(s) 528, 546, 704, 779, 924
StyI CCWWGG 2 cut(s) 93, 244
TaaI ACNGT 3 cut(s) 515, 691, 738
TaiI ACGT 3 cut(s) 833, 845, 950
TaqI TCGA 3 cut(s) 571, 594, 652
TasI AATT 4 cut(s) 378, 417, 539, 1038
TatI WGTACW 2 cut(s) 471, 510
TauI GCSGC 1 cut(s) 601
TfiI GAWTC 1 cut(s) 658
Tru1I TTAA 4 cut(s) 86, 240, 785, 1076
Tru9I TTAA 4 cut(s) 86, 240, 785, 1076
TseFI GTSAC 4 cut(s) 391, 667, 838, 964
TseI GCWGC 3 cut(s) 403, 748, 823
Tsp45I GTSAC 4 cut(s) 391, 667, 838, 964
TspDTI ATGAA 3 cut(s) 13, 130, 274
VpaK11BI GGWCC 1 cut(s) 182
VspI ATTAAT 1 cut(s) 86
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 1 cut(s) 526
XhoI CTCGAG 2 cut(s) 570, 593
XmiI GTMKAC 1 cut(s) 693
XspI CTAG 2 cut(s) 200, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.