RchiOBHm_Chr2g0130581

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001114
Physical Location & Seq
Reverse (-)
46666593 .. 46667471
879 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50204

Sequence Viewer

Length: 879 bp
ATGAAGAAACAGAAGTTGGTTAAGCAAAAATCCAACTTCTTCCAGAAAAATGGAGGGTTATTGTTGGAGCAGCATATTAATGCTTCAACCAGAGGATGTACAACAATCTTTACATCCAAGGAGCTAGAAATGACCATCAACTGTCATAAGAAGAGTCACATATTTGGTCCAGGAGATAATGGAGTCGAGCATTTCATAAATGAGATTATCACTCTTACCAAAATCAACCATCAAAATGTGGTTAAGTTATTGGGCTGCTGTCTGGTGACTGAAGCTTCTATACTGGTCTATGAACAGGCCTCTAATGGGACCCTTTTTGACTACATTTATCATACCAATGGGAAGAGTTCATTGCCTTGGGATACCCTTCTAAAGATAGCCTCAGAATCAGCAGCTGCCCTTGCTTATCTGCATTCTGCATCAATCATCAATGAAACTGTCAAGTCTTCCAACATATTGTTAACTTATTATTTTGTGGCCAAAGTCATCGGTTTTGGACCTTCGAGGTTAGTGCCTAGTAATGAATCACAGATAAGCACATTGGTTCGGAGGACACTAGGCTATTTTCATAAAGGCCAATTGACAGGTAAGAGCGATGTTTACAGCTTTGGAATGGTTTTGTTAGAGATTGTAACAGGAGAGAAGCCAATATCATTTGGTAGACCAGAAAGTCAAAGAATCATGTCTTCCCATTTTGTATCGTCCATGGAGTTACAAAGTGGTCTGTTTGATCAGATTGTTGGGTCACAAGTCATAAAACAGGGACACAGAGAACAAGTCAGAGCAGTAGCTGAGCTTGCAAAGAGATGCCTCAGTTTGAGTAGTGCAGAAAGACCTGCTATGGAAGAAGTGGCCAGGAAGTTGAAAATGTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

292

Amino Acids

32.4

Weight (kDa)

9.27

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 59 - 285 5.5e-25 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 61 - 288 1e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 669
Acc36I ACCTGC 1 cut(s) 844
AccI GTMKAC 1 cut(s) 661
AcoI YGGCCR 2 cut(s) 477, 852
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 1 cut(s) 100
AfiI CCNNNNNNNGG 1 cut(s) 306
AgsI TTSAA 2 cut(s) 87, 865
AjnI CCWGG 2 cut(s) 169, 854
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 6 cut(s) 124, 275, 395, 606, 791, 796
AluI AGCT 6 cut(s) 124, 275, 395, 606, 791, 796
AlwNI CAGNNNCTG 2 cut(s) 395, 791
AoxI GGCC 4 cut(s) 297, 477, 574, 852
ApeKI GCWGC 4 cut(s) 70, 255, 392, 395
AseI ATTAAT 1 cut(s) 78
AspS9I GGNCC 3 cut(s) 167, 309, 497
AsuHPI GGTGA 1 cut(s) 277
AvaII GGWCC 3 cut(s) 167, 309, 497
BalI TGGCCA 2 cut(s) 479, 854
BarI GAAGNNNNNNTAC 2 cut(s) 264, 296
BbsI GAAGAC 2 cut(s) 438, 678
BbvI GCAGC 4 cut(s) 82, 242, 382, 404
BccI CCATC 2 cut(s) 143, 237
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 2 cut(s) 171, 856
BciVI GTATCC 1 cut(s) 355
BclI TGATCA 1 cut(s) 730
BfaI CTAG 3 cut(s) 125, 516, 557
BfuAI ACCTGC 1 cut(s) 844
BfuI GTATCC 1 cut(s) 355
BisI GCNGC 4 cut(s) 71, 256, 393, 396
BlpI GCTNAGC 1 cut(s) 792
BlsI GCNGC 4 cut(s) 72, 257, 394, 397
Bme1390I CCNGG 2 cut(s) 171, 856
Bme18I GGWCC 3 cut(s) 167, 309, 497
BmgT120I GGNCC 3 cut(s) 167, 309, 497
BmiI GGNNCC 2 cut(s) 310, 311
BmrFI CCNGG 2 cut(s) 171, 856
BmsI GCATC 2 cut(s) 428, 797
BpiI GAAGAC 2 cut(s) 438, 678
Bpu1102I GCTNAGC 1 cut(s) 792
BsaJI CCNNGG 3 cut(s) 117, 356, 705
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 2 cut(s) 171, 856
BseDI CCNNGG 3 cut(s) 117, 356, 705
BseGI GGATG 2 cut(s) 101, 113
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 3 cut(s) 396, 783, 826
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 4 cut(s) 82, 242, 382, 404
BsgI GTGCAG 1 cut(s) 846
BshFI GGCC 4 cut(s) 299, 479, 576, 854
BslFI GGGAC 2 cut(s) 322, 777
BslI CCNNNNNNNGG 1 cut(s) 306
BsmFI GGGAC 2 cut(s) 322, 777
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 4 cut(s) 299, 479, 576, 854
Bsp1407I TGTACA 1 cut(s) 98
Bsp143I GATC 1 cut(s) 730
Bsp1720I GCTNAGC 1 cut(s) 792
Bsp19I CCATGG 1 cut(s) 705
BspANI GGCC 4 cut(s) 299, 479, 576, 854
BspCNI CTCAG 3 cut(s) 395, 784, 825
BspLI GGNNCC 2 cut(s) 310, 311
BspMI ACCTGC 1 cut(s) 844
BsrDI GCAATG 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 98
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 3 cut(s) 117, 356, 705
BssMI GATC 1 cut(s) 730
BssT1I CCWWGG 3 cut(s) 117, 356, 705
Bst2UI CCWGG 2 cut(s) 171, 856
Bst4CI ACNGT 2 cut(s) 143, 439
Bst6I CTCTTC 2 cut(s) 146, 338
BstAUI TGTACA 1 cut(s) 98
BstC8I GCNNGC 1 cut(s) 798
BstDEI CTNAG 3 cut(s) 382, 792, 812
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 2 cut(s) 101, 113
BstKTI GATC 1 cut(s) 733
BstMBI GATC 1 cut(s) 730
BstMWI GCNNNNNNNGC 2 cut(s) 401, 797
BstNI CCWGG 2 cut(s) 171, 856
BstSCI CCNGG 2 cut(s) 169, 854
BstV1I GCAGC 4 cut(s) 82, 242, 382, 404
BstV2I GAAGAC 2 cut(s) 438, 678
BstXI CCANNNNNNTGG 1 cut(s) 50
BsuI GTATCC 1 cut(s) 355
BsuRI GGCC 4 cut(s) 299, 479, 576, 854
BtgI CCRYGG 1 cut(s) 705
BtgZI GCGATG 1 cut(s) 609
BtsCI GGATG 2 cut(s) 101, 113
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 1 cut(s) 798
CaiI CAGNNNCTG 2 cut(s) 395, 791
Cfr13I GGNCC 3 cut(s) 167, 309, 497
Csp6I GTAC 1 cut(s) 99
CviAII CATG 2 cut(s) 682, 706
CviQI GTAC 1 cut(s) 99
DdeI CTNAG 3 cut(s) 382, 792, 812
DpnI GATC 1 cut(s) 732
DpnII GATC 1 cut(s) 730
DrdI GACNNNNNNGTC 1 cut(s) 669
DseDI GACNNNNNNGTC 1 cut(s) 669
EaeI YGGCCR 2 cut(s) 477, 852
Eam1104I CTCTTC 2 cut(s) 146, 338
EarI CTCTTC 2 cut(s) 146, 338
Eco130I CCWWGG 3 cut(s) 117, 356, 705
Eco147I AGGCCT 1 cut(s) 299
Eco47I GGWCC 3 cut(s) 167, 309, 497
Eco57I CTGAAG 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 169, 854
EcoT14I CCWWGG 3 cut(s) 117, 356, 705
ErhI CCWWGG 3 cut(s) 117, 356, 705
FaeI CATG 2 cut(s) 685, 709
FaqI GGGAC 2 cut(s) 322, 777
FatI CATG 2 cut(s) 681, 705
FbaI TGATCA 1 cut(s) 730
FblI GTMKAC 1 cut(s) 661
Fnu4HI GCNGC 4 cut(s) 71, 256, 393, 396
FokI GGATG 2 cut(s) 100, 108
Fsp4HI GCNGC 4 cut(s) 71, 256, 393, 396
FspBI CTAG 3 cut(s) 125, 516, 557
GluI GCNGC 4 cut(s) 71, 256, 393, 396
HaeIII GGCC 4 cut(s) 299, 479, 576, 854
Hin1II CATG 2 cut(s) 685, 709
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HindIII AAGCTT 1 cut(s) 273
HinfI GANTC 5 cut(s) 154, 183, 386, 524, 678
HpaI GTTAAC 1 cut(s) 462
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 3 cut(s) 462, 601, 662
Hpy188I TCNGA 4 cut(s) 385, 549, 735, 782
Hpy188III TCNNGA 1 cut(s) 43
Hpy8I GTNNAC 3 cut(s) 462, 601, 662
HpyAV CCTTC 2 cut(s) 377, 510
HpyCH4III ACNGT 2 cut(s) 143, 439
HpyCH4V TGCA 4 cut(s) 412, 419, 800, 827
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 797
HpyF3I CTNAG 3 cut(s) 382, 792, 812
Hsp92II CATG 2 cut(s) 685, 709
KflI GGGWCCC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 730
KspAI GTTAAC 1 cut(s) 462
Kzo9I GATC 1 cut(s) 730
LmnI GCTCC 2 cut(s) 67, 121
Lsp1109I GCAGC 4 cut(s) 82, 242, 382, 404
LweI GCATC 2 cut(s) 428, 797
MaeI CTAG 3 cut(s) 125, 516, 557
MaeIII GTNAC 5 cut(s) 155, 265, 631, 711, 744
MalI GATC 1 cut(s) 732
MboI GATC 1 cut(s) 730
MboII GAAGA 7 cut(s) 16, 31, 163, 355, 438, 678, 857
MfeI CAATTG 1 cut(s) 578
MlsI TGGCCA 2 cut(s) 479, 854
MluCI AATT 1 cut(s) 578
MluNI TGGCCA 2 cut(s) 479, 854
MlyI GAGTC 2 cut(s) 163, 192
MmeI TCCRAC 3 cut(s) 45, 57, 474
MnlI CCTC 7 cut(s) 47, 86, 310, 391, 498, 543, 821
Mox20I TGGCCA 2 cut(s) 479, 854
MscI TGGCCA 2 cut(s) 479, 854
MseI TTAA 4 cut(s) 21, 78, 243, 461
MslI CAYNNNNRTG 3 cut(s) 78, 234, 336
Msp20I TGGCCA 2 cut(s) 479, 854
MspA1I CMGCKG 1 cut(s) 395
MspR9I CCNGG 2 cut(s) 171, 856
MunI CAATTG 1 cut(s) 578
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 2 cut(s) 171, 856
MwoI GCNNNNNNNGC 2 cut(s) 401, 797
NcoI CCATGG 1 cut(s) 705
NdeII GATC 1 cut(s) 730
NlaIII CATG 2 cut(s) 685, 709
NlaIV GGNNCC 2 cut(s) 310, 311
NmuCI GTSAC 3 cut(s) 155, 265, 744
PceI AGGCCT 1 cut(s) 299
PctI GAATGC 1 cut(s) 412
PfeI GAWTC 3 cut(s) 386, 524, 678
PfoI TCCNGGA 1 cut(s) 169
PkrI GCNGC 4 cut(s) 72, 257, 394, 397
PleI GAGTC 2 cut(s) 162, 191
PpsI GAGTC 2 cut(s) 162, 191
PpuMI RGGWCCY 1 cut(s) 309
PshBI ATTAAT 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 309
Psp6I CCWGG 2 cut(s) 169, 854
PspGI CCWGG 2 cut(s) 169, 854
PspN4I GGNNCC 2 cut(s) 310, 311
PspPI GGNCC 3 cut(s) 167, 309, 497
PspPPI RGGWCCY 1 cut(s) 309
PstNI CAGNNNCTG 2 cut(s) 395, 791
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 1 cut(s) 100
RsaNI GTAC 1 cut(s) 99
RseI CAYNNNNRTG 3 cut(s) 78, 234, 336
SaqAI TTAA 4 cut(s) 21, 78, 243, 461
SatI GCNGC 4 cut(s) 71, 256, 393, 396
Sau3AI GATC 1 cut(s) 730
Sau96I GGNCC 3 cut(s) 167, 309, 497
SchI GAGTC 2 cut(s) 163, 192
ScrFI CCNGG 2 cut(s) 171, 856
SfaNI GCATC 2 cut(s) 428, 797
SinI GGWCC 3 cut(s) 167, 309, 497
SmiMI CAYNNNNRTG 3 cut(s) 78, 234, 336
Sse9I AATT 1 cut(s) 578
SseBI AGGCCT 1 cut(s) 299
SspMI CTAG 3 cut(s) 125, 516, 557
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 169, 854
StyI CCWWGG 3 cut(s) 117, 356, 705
TaaI ACNGT 2 cut(s) 143, 439
TaqI TCGA 2 cut(s) 186, 503
TasI AATT 1 cut(s) 578
TatI WGTACW 1 cut(s) 98
TfiI GAWTC 3 cut(s) 386, 524, 678
Tru1I TTAA 4 cut(s) 21, 78, 243, 461
Tru9I TTAA 4 cut(s) 21, 78, 243, 461
TseFI GTSAC 3 cut(s) 155, 265, 744
TseI GCWGC 4 cut(s) 70, 255, 392, 395
Tsp45I GTSAC 3 cut(s) 155, 265, 744
TspDTI ATGAA 7 cut(s) 17, 184, 306, 339, 447, 537, 557
VpaK11BI GGWCC 3 cut(s) 167, 309, 497
VspI ATTAAT 1 cut(s) 78
XmiI GTMKAC 1 cut(s) 661
XspI CTAG 3 cut(s) 125, 516, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.